Raman spectroscopy. Mr/ Abd El mo’ez Ahmed Mohammed “3th group” “Neutron physics and...
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Transcript of Raman spectroscopy. Mr/ Abd El mo’ez Ahmed Mohammed “3th group” “Neutron physics and...
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Raman spectroscopy Raman spectroscopy
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Mr/ Abd El mo’ez Ahmed Mohammed “3th group”
“Neutron physics and application in material science and nuclear
application”physics departmentFaculty of ScienceSohag University
Egypt
Faculty of Science
Physics Department
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JINR’s group
Supervisor : prof . S.I. Tutunnikov.
Technician : Mr. Kovalev Yura.
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Why we use Raman spectroscopy?
Raman spectroscopy is used to
determine the molecular motions,
especially the vibrational one .
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Now what is the application?Now what is the application?
We can use Raman spectroscopy in:-We can use Raman spectroscopy in:- Tissue characterization.Tissue characterization.
Tissue Imaging.Tissue Imaging.
Live Cell Imaging.Live Cell Imaging.
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Tissue characterizationTissue characterization
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Tissue Imaging
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Live Cell ImagingAll the sexiness, without the dyes!!All the sexiness, without the dyes!!
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h
h(-+) 1)
h 3
2
1
0 S1
3
2
1
0 S0
Ene
rgy
Virtual Level
Rayleigh Raman (inelastic)(elastic) Scattering Scattering
Inelastic Scattering
difference in energy
• Energy transferred from incident light to molecular vibrations
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Raman Spectrometer Main view of SOLAR-TII setup
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Raman spectrometer’s mechanism.
The laser beam falls on the filter and then it passes to the objective and collides with the Sample.The light will be scattered in all directions and will be reflected to the objective again. After the reflected light reaches the objective it falls down on the spectrometer system and finally to theCCD or PMT detectors.
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Principle of Confocal Microscopy
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Experimental data of a ceramic material.
400 450 500 550 600 650 700 750
-200
0
200
400
600
Inte
nsity
Wave lenght (nm)
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Acknowledgement
Finally I’d like to thank
1- Mr/ Kovalev Yura.
2- Mr/ Valantine.
for their help and efforts.