Quiz#10 LC71010/27/10name___________
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Quiz#10 LC710 10/27/10 name_________ Given the following transgenes co-existing in the same animal. CMVp rtta I.R.E.S GFP tetO RFP Cellular Fluorescence observed prior to addition of Doxycycline is: Red Green Both Neither 1 0.5 pt (circle one) : Q2 0.5 pt (circle one) : TATA rtta binds DNA if Dox is present t What does tta do in the absence of Dox?________________________ What does tta do in the presence of Dox?________________________ Cellular Fluorescence observed after addition of Doxycycline is: Red Green Both Neither
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Quiz#10 LC71010/27/10name___________. Given the following transgenes co-existing in the same animal. rtta binds DNA if Dox is present. CMVp. rtta I.R.E.S GFP. tetO. TATA. RFP. Q1 0.5 pt (circle one) :. Q2 0.5 pt (circle one) :. Cellular Fluorescence observed - PowerPoint PPT Presentation
Transcript of Quiz#10 LC71010/27/10name___________
Quiz#10 LC710 10/27/10 name___________
Given the following transgenes co-existing in the same animal.
CMVp
rtta I.R.E.S GFP
tetO
RFP
Cellular Fluorescence observedprior to addition of Doxycycline is:
RedGreenBothNeither
Q1 0.5 pt (circle one) : Q2 0.5 pt (circle one) :
TATA
rtta binds DNA if Dox is present
Q3: 1pt What does tta do in the absence of Dox?________________________ What does tta do in the presence of Dox?________________________
Cellular Fluorescence observedafter addition of Doxycycline is:
RedGreenBothNeither
Pax6p
CreERt2 I.R.E.S GFP
loxP-RFP-loxP-TOXIN
ATAACTTCGTATAATGTATGCTATACGAAGTTAT = loxP sequence
Hox1p
Prior to addition of TAM, which Cells are:
1
2
3
4
5
6
7
8
9
10
11
12
Red only:Green only:Both:Neither:
Above are WT cells inthe brain expressing Pax6and/or Hox1Hox1.
After TAM addition, which Cells are:
If expressed, TOXIN will Kill cells (disappear)
Red only:Green only:Both:Neither:
Given the following transgenes co-existing in the same animal.
Q4 (2pts) Q5 (2pts)
Q6 (2pts) : in the above transgene, loxP sites are in this orientation:
This experiment is actually poorly designed and the loxP sites need to oriented as follows:
Hox1p
Why?
TAM= TAMOXIFEN
