QuantStudio 3D AnalysisSuite Software · QuantStudio™ 3D AnalysisSuite™ Software Help Welcome...

HELP

For Research Use Only. Not for use in diagnostic procedures.

QuantStudio™ 3D AnalysisSuite™ Softwarefor use with QuantStudio™ 3D Digital PCR System and QuantStudio™ 3DAnalysisSuite™ Server System

Publication Number MAN0009634

Revision A.0

For Research Use Only. Not for use in diagnostic procedures.

The information in this guide is subject to change without notice.

DISCLAIMER

LIFE TECHNOLOGIES CORPORATION AND/OR ITS AFFILIATE(S) DISCLAIM ALL WARRANTIES WITH RESPECT TO THIS DOCUMENT, EXPRESSED ORIMPLIED, INCLUDING BUT NOT LIMITED TO THOSE OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, OR NON-INFRINGEMENT. TOTHE EXTENT ALLOWED BY LAW, IN NO EVENT SHALL LIFE TECHNOLOGIES AND/OR ITS AFFILIATE(S) BE LIABLE, WHETHER IN CONTRACT, TORT,WARRANTY, OR UNDER ANY STATUTE OR ON ANY OTHER BASIS FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE OR CONSEQUENTIALDAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING BUT NOT LIMITED TO THE USE THEREOF.

Trademarks

Microsoft, Windows, and Internet Explorer are registered trademarks of Microsoft Corporation. TaqMan is a registered trademark of Roche MolecularSystems, Inc., used under permission and license. Google is a registered trademark and Chrome is a trademark of Google, Inc. Adobe and Reader areregistered trademarks of Adobe Systems, Inc. RedHat is a registered trademark of Red Hat, Inc. Linux is a registered trademark of Linus Torvalds.Dell and PowerEdge are trademarks of Dell, Inc. Mozilla and Firefox are registered trademarks of the Mozilla Foundation. All other trademarks arethe property of Thermo Fisher Scientific and its subsidiaries.

Life Technologies is a Thermo Fisher Scientific brand. ©2014 Thermo Fisher Scientific Inc. All rights reserved.

Contents

QuantStudio™ 3D AnalysisSuite™ Software Help . . . . . . . . . . . . . . . . . . . . . . . . 5

■ About this Help system .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

Revision history . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

Purpose . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

Prerequisites . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

Obtain information from the Help system . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

■ CHAPTER 1 About the system .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

About the QuantStudio™ 3D Digital PCR System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

■ CHAPTER 2 About digital PCR experiments . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

What is a digital PCR experiment? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

Elements of dPCR experiments . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

Digital PCR experiment setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

■ CHAPTER 3 About the QuantStudio™ 3D AnalysisSuite™ Software. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

Computer requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

Compatible instruments . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11QuantStudio™ 3D System data flow . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11About the data files . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

About the analysis modules . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

General analysis workflow . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

Manage users in the AnalysisSuite™ Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12Create new users . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12Update existing users . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

Log into the software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

Manage your profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14Edit your profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14Log out of the software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

Select an analysis module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

QuantStudio™ 3D AnalysisSuite™ Software Help 3

■ Documentation and support . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

Related documentation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

Obtaining support . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

Limited product warranty . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

Contents

4 QuantStudio™ 3D AnalysisSuite™ Software Help

QuantStudio™ 3D AnalysisSuite™ SoftwareHelp

Welcome to the QuantStudio™ 3D AnalysisSuite™ Software Help.

The server-based AnalysisSuite™ Software is a secure, online tool providing nextgeneration digital PCR (dPCR) data analysis within several application-specificanalysis modules. The AnalysisSuite™ Software analysis modules integrate dPCR datafrom QuantStudio™ 3D Digital PCR 20K Chips run on the QuantStudio™ 3D DigitalPCR Instrument with bioinformatics to provide simple, easy to interpret results forapplications such as pathogen detection, viral load, and copy number variation. Youcan then view, print, and download graphs and tables of the results from eachanalysis module.

To start, select an analysis module from the list of available modules.

Use the Contents in the left navigation pane to find your topic of interest, or enteryour search term(s) in the Search field above (see “Obtain information from the Helpsystem” on page 6).


About this Help system

Revision history

Revision Date Description Pub. no.

A.0 February2014

New Product- help documentationfor use and navigation of the server-based QuantStudio™ 3DAnalysisSuite™ Software designed toanalyze digital PCR experiments.

MAN0009634

Purpose

This Help system describes the server-based QuantStudio™ 3D AnalysisSuite™

Software and provides procedures for common administrative tasks.

Prerequisites

This Help system assumes that you have access to and are familiar with theprocedures provided in the QuantStudio™ 3D Digital PCR System User Guide (see “Related documentation” on page 15).

This Help system also assumes that you have a general understanding of:• Data storage, file transfers, and copying and pasting.• The Red Hat® Enterprise Linux® and Microsoft® Windows® operating systems,

the Internet, and Internet-based browsers.

Obtain information from the Help system

The QuantStudio™ 3D AnalysisSuite™ Software features a Help system that describeshow to use each feature of the user interface. You can use the Help system to findtopics of interest by:

• Reviewing the table of Contents in the sidebar.• Searching for a specific topic – Enter your search term in the Search field in the

upper right, then click Enter. The search results will appear under the Search tabin the sidebar.

To access the Help system click from any screen.


About the system

About the QuantStudio™ 3D Digital PCR System

The QuantStudio™ 3D Digital PCR System uses fluorescent-labeled, probe-basedquantitative polymerase chain reaction (PCR) reagents to provide quantitative andqualitative research detection of target nucleic acid sequences (targets) using post-PCR (endpoint) analysis. The QuantStudio™ 3D Instrument performs imaging andpreliminary analysis of QuantStudio™ 3D Digital PCR 20K Chips that have beenloaded with TaqMan® assays and thermal cycled using a Dual Flat Block GeneAmp®

PCR System 9700. Subsequent analysis and post-processing is performed by theQuantStudio™ 3D AnalysisSuite™ Software to yield relative or absolute quantificationresults from the raw imaging data.

The following figure lists the components of the QuantStudio™ 3D System.

F1 F2 F3 F4

1 2 3

4 5 6

7 8 9

ENTER

STOP

0 CE

F5

GeneAmp ®

PCR System 9700

POWER

1

35

67

4 8

9 10

2 12

11

1 QuantStudio™ 3D Digital PCR ThermalPads

2 QuantStudio™ 3D Digital PCR ChipCarriers

3 Dual Flat Block GeneAmp® PCR System9700

4 QuantStudio™ 3D Digital PCR Instrument5 QuantStudio™ 3D Digital PCR Chip Case

Lid6 QuantStudio™ 3D Digital PCR Sample

Loading Blade7 QuantStudio™ 3D Digital PCR 20K Chip

8 (Optional) QuantStudio™ 3D Digital PCRChip Loader

9 UV-Activated Chip Sealant Syringe10 QuantStudio™ 12K Flex OpenArray®

Immersion Fluid11 QuantStudio™ 3D Digital PCR Master Mix12 TaqMan® Assays13 (Not shown) QuantStudio™ 3D Tilt Base

for Dual Flat Block GeneAmp® PCRSystem 9700

14 (Not shown) QuantStudio™ 3DAnalysisSuite™ Software

1


About digital PCR experiments

What is a digital PCR experiment?

Digital PCR (dPCR) is a molecular biology technique used to quantify the number ofstarting copies of a target nucleic acid sequence in a genomic or complementary DNAsample without the use of a standard. Digital PCR analysis requires that at least somePCR reactions within the sample replicate group have zero copies (individualreactions will contain either zero, one, or a few target molecules). Amplification isdetected in reactions receiving at least one molecule and classified as positive whileno amplification is detectable in reactions not receiving target and is converselyclassified as negative. Following the PCR, the total number of negative reactions is fitto a Poisson distribution to estimate the absolute copies of template molecules presentin the sample volume.

For concentrated samples, dilution to the single-molecule limit may be required. Fordetection of rare targets (mutant sequences, pathogens, transgene content) dilution isgenerally not required.

Applications of digital PCR include, but are not limited to, quantification of low-levelpathogens, detection of rare sequences, gene expression in single cells, and low-foldcopy number discrimination of genes/targets.

Elements of dPCR experiments

Digital PCR experiments include:

• Sample – The genomic or complementary DNA sample that contains anunknown number of copies of the target nucleic acid sequence. In a digital PCRexperiment samples are diluted down to a limiting quantity, such that onaverage, individual PCR reactions contain a single target molecule.

• QuantStudio™ 3D Digital PCR Master Mix – An optimized mixture of dNTPs,salt, buffer, DNA polymerase, and ROX™passive reference dye designed for usewith TaqMan® assays and the QuantStudio™ 3D Digital PCR System.

• TaqMan® assay – A mixture that includes forward and reverse primers and oneor more specific fluorescent dye-labeled probes for the target nucleic acidsequence.

• (Optional) Technical replicates – Chips that contain identical sample/assay/reaction mix combinations and volumes. Each QuantStudio™ 3D Digital PCR 20KChip contains up to 20,000 reaction wells (resulting from the loading of a singlePCR reaction/sample).

2


• (Optional) Negative controls (NTCs) – Samples that should not amplify.– No template controls – Samples that contain water or buffer instead of

template.– No target controls – Samples that contain genetic material but not the target

template.

IMPORTANT! You can identify a negative control in the QuantStudio™ 3DAnalysisSuite™ Software by naming a Sample "NTC" or something similar. TheSoftware ‘treats’ the NTC-named samples in the same way as the other samples.You need to look out for any amplification in the NTC-named samples as thesoftware does not produce a flag if amplification occurs in those reaction wells.

Digital PCR experiment setup

In a digital PCR experiment performed on the QuantStudio™ 3D Digital PCR System,dilutions of each gDNA or cDNA sample are loaded into the wells of a QuantStudio™

3D Digital PCR 20K Chip that contains QuantStudio™ 3D Digital PCR Master Mix andTaqMan® assay appropriate for your experiment. If the target concentration is high,samples must be diluted down to a limiting quantity prior to assembly of the reactionmix in the Digital PCR 20K Chip, such that a portion of the individual PCR reactionsreceive no target molecules.

Chapter 2 About digital PCR experimentsDigital PCR experiment setup 2


About the QuantStudio™ 3DAnalysisSuite™ Software

■ Computer requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

■ Compatible instruments . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

■ About the analysis modules . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

■ General analysis workflow . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

■ Manage users in the AnalysisSuite™ Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

■ Log into the software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

■ Manage your profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

■ Select an analysis module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

The QuantStudio™ 3D AnalysisSuite™ Software performs statistical analysis of digitalPCR experiments and can be used to calculate the absolute or relative number oftarget nucleic acid sequences in a biological sample.

Computer requirements

When deployed in server configuration, the QuantStudio™ 3D AnalysisSuite™

Software is accessed as a web-based application that can be run using any Internetbrowser that meets the hardware and operating system requirements in the followingtable:

Hardware Operatingsystem

Browser

Google®

Chrome™[1]

Microsoft®

InternetExplorer®

Mozilla®

Firefox®

Client computer Microsoft®

Windows® 7v21 or later v10.0 or later None[2]

Servercomputer (Dell™

PowerEdge™

T110 II server)

Red Hat®

EnterpriseLinux®

v21 or later None[3] v10.0.12 or later

[1] Recommended browser for all platforms[2] Browser not supported for this platform[3] Browser not available for this platform

3


Compatible instruments

AnalysisSuite™ Software can be used to analyze the results of digital PCR experimentsrun on the QuantStudio™ 3D Instrument that have been exported as experiment data(.eds) files.

Note: Refer to the QuantStudio™ 3D Digital PCR System User Guide (Pub. no.MAN0007720) for detailed information on the instrument operational and dataworkflow.

For each imaged Digital PCR 20K Chip, the QuantStudio™ 3D Instrument generatesand saves a single experiment (.eds) file that contains the processed imaging data andthe results from the preliminary analysis on the instrument. Depending on the chosendata destination, the instrument transfers the saved imaging data directly to theAnalysisSuite™ Server System or Life Technologies™ cloud data storage service forimmediate access by the AnalysisSuite™ Software, or to a network file server or otherexternal storage device such as a USB drive for later manual import.

The instrument names each experiment file uniquely by combining the ID of the chipand the date/time of the run according to the following formula:

BO498N_130531_195817.eds

2 31

1 Chip ID - The label applied to theQuantStudio™ 3D Digital PCR Chip CaseLid that uniquely identifies the DigitalPCR 20K Chip to which it is applied.

X0300036 Chip ID

IMPORTANT! If the instrument cannotread the ID of a loaded chip, then it usesthe instrument name instead to namethe file.

2 Date stamp – The date at which theDigital PCR 20K Chip was run, expressedin the "yymmdd" format where yy is thelast two digits of the year, mm is thenumerical order of the month, and dd isthe day of the run.

3 Time stamp – The time at which theDigital PCR 20K Chip was run, expressedin the "hhmmss" format where hh is thehour (in 24-hour format), mm is theminutes, and ss is the seconds.

Note: In the example shown above, the file name indicates that the file contains datafor chip BO498N which was run at 7:58 pm on May 31, 2013.

About the analysis modules

The Absolute Quantification and Relative Quantification analysis modules of theQuantStudio™ 3D AnalysisSuite™ generate calls for the reaction wells on each chipthat contain viable PCR products. Using the call data, the software calculates copynumber values for the sample present on the chip and generates confidence intervals.The copy number values are generated using the maximum-likelihood estimator forquantity in the Poisson distribution and the confidence interval is given by theFazekas de St. Groth algorithm (Fazekas de St. Groth, S., 1982). When combining

QuantStudio™ 3DSystem data flow

About the datafiles

About the QuantStudio™ 3D AnalysisSuite™ SoftwareCompatible instruments 3


replicates and dilution series, the software calculates a single quantification result andgenerates a confidence interval for that result.

General analysis workflow

The following figure shows the general workflow for analyzing digital PCRexperiments using the AnalysisSuite™ Software. Both Absolute Quantification (singlereporter) and Relative Quantification (dual reporter) experiments follow the samegeneral analysis procedure.

START

q

Create a project

q

Import chip data

q

Define chip settings (optional)

q

Review chip quality and calls (optional)

q

Review chip results and project results

q

Export project data

q

FINISH

Manage users in the AnalysisSuite™ Software

Only users with an Administrator account in the AnalysisSuite™ Software can createand enable new user accounts.

An Administrator must fill out the information in the Manage Users form and create aUser Name and Password for each user that will use the server-based AnalysisSuite™

Software. The User Name and Password will give each user secure access to all theprojects they create in the software. The projects in the private account for each userwill only be accessible using their particular User Name and Password.

1. Log into the software as an Administrator.

2. Click the administrator User Name at the top of the main window, then selectManage User.

Create new users

About the QuantStudio™ 3D AnalysisSuite™ SoftwareGeneral analysis workflow3


3. Click Create new user, then complete the required user profile information:• User Name (email address) – This email address must be unique to this

particular account; the same email address cannot be used for more than oneaccount. This address will be used to contact the user with importantinformation about their account. In the event of a lost password, this addresswill be used to notify the user of the new password.

• Password – This must be at least eight characters in length and should be acombination of letters and at least one number for maximum security.

• (Optional) First Name and Last Name – Names can be any combination ofletters and numbers.

• User Type – Select one of the available user roles:– Admin – In addition to the End-user tasks, this role can manage (create

and update) other users, and maintain the server system (backup andrestore) and AnalysisSuite™ Software (install and uninstall).

– End-user (default) – This role can use the AnalysisSuite™ Software toanalyze chips (import data, create and analyze projects, and exportdata).

• Enabled (Yes/No) – Select the checkbox to give this account secure accessto the software. This checkbox is set to No by default.

Note: For optimal performance, access to the software should be limited toa maximum of 35 concurrent users. To block user access to the software,deselect this checkbox.

4. Click Update to save the user profile or click Cancel to discard the changes.

5. To return to the homepage at any time, click QuantStudio™ 3D AnalysisSuite™

in the Life Technologies™ banner.

1. Log into the software as an Administrator.

2. Click the administrator User Name at the top of the main window, then selectManage User.

3. For a particular user profile in the Manage Users screen:

• Edit the user profile – Click Edit, make the desired changes (see “Create newusers” on page 12), then click Update to save the changes or click Cancel todiscard the changes.

• Delete the user – Click Delete, then click Delete to confirm the action.

IMPORTANT! Deleting the user will also delete all of the projects created bythis user in any of the available AnalysisSuite™ Software analysis modules.Click Cancel, then navigate to and export any projects by this user before youcontinue. Refer to the QuantStudio™ 3D AnalysisSuite™ Software Help for ananalysis module for instructions on how to export projects from the software.

Note: You cannot delete the default administrator user profile.



Update existingusers

About the QuantStudio™ 3D AnalysisSuite™ SoftwareManage users in the AnalysisSuite™ Software 3


Log into the software

Follow the instructions in the QuantStudio™ 3D AnalysisSuite™ Server System QuickReference to log into the AnalysisSuite™ Software using the user name and passwordyou received from your server administrator.

Manage your profile

To change the information in your user profile, including your User Name andPassword:

1. Click your User Name at the top of the main window, then select Your Profile.

2. Make the desired changes, then click Save.



To log out of the AnalysisSuite™ Software:

1. Click your User Name at the top of the main window.

2. Select Sign Out.

Select an analysis module

1. Follow the instructions in the QuantStudio™ 3D AnalysisSuite™ Server System QuickReference to log into the QuantStudio™ 3D AnalysisSuite™ using the user nameand password you received from your server administrator.

2. Log into the software (see “Log into the software” on page 14).

3. Select an analysis module from the QuantStudio™ 3D AnalysisSuite™ Softwarehomepage.

Note: When prompted upon first login to an analysis module, click to Acceptthe terms of the End User License Agreement.

Note: The software opens to the Project Listing screen for the selected analysismodule. To select a different analysis module, click QuantStudio™ 3DAnalysisSuite™ at the top of any screen in the software.

Edit your profile

Log out of thesoftware

About the QuantStudio™ 3D AnalysisSuite™ SoftwareLog into the software3


Documentation and support

Related documentation

Document Publicationnumber

Description

QuantStudio™ 3D Digital PCRSystem User Guide

MAN0007720 Describes the QuantStudio™ 3DDigital PCR System hardware andsoftware and provides informationon preparing, maintaining, andtroubleshooting the system.

QuantStudio™ 3D Digital PCRSystem Quick Reference

MAN0008159 Describes the QuantStudio™ 3DDigital PCR System hardware andsoftware and provides brief, step-by-step procedures for commontasks.

QuantStudio™ 3D AnalysisSuite™

Server System Quick Reference4486248 Describes the AnalysisSuite™

Server System hardware andsoftware and provides informationon installing the system and brief,step-by-step procedures forcommon tasks.

Portable document format (PDF) versions of related documentation are available at http://www.lifetechnologies.com/quantstudio3d.

Note: To open the user documentation, use the Adobe® Reader® software availablefrom www.adobe.com

Note: For additional documentation or if you cannot access the user documentation,see “Obtaining support” on page 15.

Obtaining support

For the latest services and support information for all locations, go to:

www.lifetechnologies.com/support

At the website, you can:• Access worldwide telephone and fax numbers to contact Technical Support and

Sales facilities• Search through frequently asked questions (FAQs)


http://www.lifetechnologies.com/quantstudio3d

http://www.adobe.com

http://www.lifetechnologies.com/support

• Submit a question directly to Technical Support ([email protected])• Search for user documents, SDSs, vector maps and sequences, application notes,

formulations, handbooks, certificates of analysis, citations, and other productsupport documents

• Obtain information about customer training• Download software updates and patches

Limited product warranty

Life Technologies Corporation and/or its affiliate(s) warrant their products as set forthin the Life Technologies' General Terms and Conditions of Sale found on LifeTechnologies' website at www.lifetechnologies.com/termsandconditions. If you haveany questions, please contact Life Technologies at www.lifetechnologies.com/support.

Documentation and supportLimited product warranty


mailto:[email protected]

http://www.lifetechnologies.com/termsandconditions



Headquarters5791 Van Allen Way | Carlsbad, CA 92008 USA | Phone +1 760 603 7200 | Toll Free in USA 800 955 6288For support visit lifetechnologies.com/support or email [email protected]

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19 February 2014

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