Epistasis and quantitative traits: using model organisms ...
Quantitative traits of host-pathogen interaction, and ... › fileadmin ›...
Transcript of Quantitative traits of host-pathogen interaction, and ... › fileadmin ›...
![Page 1: Quantitative traits of host-pathogen interaction, and ... › fileadmin › 11_Henriette_Goyeau_pp.pdf · Quantitative traits of host-pathogen interaction, and their contribution](https://reader034.fdocuments.us/reader034/viewer/2022042322/5f0bfc2c7e708231d43330d0/html5/thumbnails/1.jpg)
Quantitative traits of host-pathogen
interaction, and their contribution to
epidemic development in the wheat leaf
rust pathosystem.
H. Goyeau, G. Azzimonti, J. Papaïx, C. Lannou
![Page 2: Quantitative traits of host-pathogen interaction, and ... › fileadmin › 11_Henriette_Goyeau_pp.pdf · Quantitative traits of host-pathogen interaction, and their contribution](https://reader034.fdocuments.us/reader034/viewer/2022042322/5f0bfc2c7e708231d43330d0/html5/thumbnails/2.jpg)
Resistance components Aggressiveness components
Disease
HOST PATHOGEN
Quantitative traits of the host-pathogen
interaction
![Page 3: Quantitative traits of host-pathogen interaction, and ... › fileadmin › 11_Henriette_Goyeau_pp.pdf · Quantitative traits of host-pathogen interaction, and their contribution](https://reader034.fdocuments.us/reader034/viewer/2022042322/5f0bfc2c7e708231d43330d0/html5/thumbnails/3.jpg)
Assessment of quantitative traits
Growth Sporulation Infection
…In the glasshouse
A B
LP Latent Period SPL
SPS
LS
Sporulation per lesion
Per unit area
Lesion Size
40x
IE Infection Efficiency
![Page 4: Quantitative traits of host-pathogen interaction, and ... › fileadmin › 11_Henriette_Goyeau_pp.pdf · Quantitative traits of host-pathogen interaction, and their contribution](https://reader034.fdocuments.us/reader034/viewer/2022042322/5f0bfc2c7e708231d43330d0/html5/thumbnails/4.jpg)
Resistance components Aggressiveness components
HOST PATHOGEN
Disease
Quantitative traits of the host-pathogen
interaction
Questions :
• Relationships between different traits • Relationships between traits and field disease severity?
![Page 5: Quantitative traits of host-pathogen interaction, and ... › fileadmin › 11_Henriette_Goyeau_pp.pdf · Quantitative traits of host-pathogen interaction, and their contribution](https://reader034.fdocuments.us/reader034/viewer/2022042322/5f0bfc2c7e708231d43330d0/html5/thumbnails/5.jpg)
0
10
20
30
40
50
1999 2000 2001 2002 2003 2004 2005 2006 2007 2008
P3 P4 P5
Fréquence(%)
SID
CIE
CAMINS
BUS
FRA
LD7
AND
TRE
BAL
PBI
ECR
SOI
APA
0
0.2
0.4
0.6
0.8
1
RA
UD
PC
SID
CIE
CAMINS
BUS
FRA
LD7
AND
TRE
BAL
PBI
ECR
SOI
APA
0
0.2
0.4
0.6
0.8
1
RA
UD
PC
X
Experimental settings
13 Cultivars 3 PATHOTYPES
P3 P4 P5
2009 2010 2011
FIELD
3 years, 3 assessments per year
GLASSHOUSE
Quantitaive Traits (IE,
Latency, Sporulation)
![Page 6: Quantitative traits of host-pathogen interaction, and ... › fileadmin › 11_Henriette_Goyeau_pp.pdf · Quantitative traits of host-pathogen interaction, and their contribution](https://reader034.fdocuments.us/reader034/viewer/2022042322/5f0bfc2c7e708231d43330d0/html5/thumbnails/6.jpg)
Data Analysis
Distribution of variables
Bayesian Modelling -> Posterior Distribution
Regression to the estimated values
• Between quantitative traits • Between quantitative traits and field disease severity
• Quantitative traits (glasshouse) • Disease severity (field)
Correlations
PCA
Multiple colinearities
![Page 7: Quantitative traits of host-pathogen interaction, and ... › fileadmin › 11_Henriette_Goyeau_pp.pdf · Quantitative traits of host-pathogen interaction, and their contribution](https://reader034.fdocuments.us/reader034/viewer/2022042322/5f0bfc2c7e708231d43330d0/html5/thumbnails/7.jpg)
Simple linear Relationships between quantitative traits
LP
IE
SPS
IE
Positive rel. => increased R
LS
SPS
Negative rel. => Trade Off
SPL
SPS
NS (P=0,245)
Most of the components were correlated
![Page 8: Quantitative traits of host-pathogen interaction, and ... › fileadmin › 11_Henriette_Goyeau_pp.pdf · Quantitative traits of host-pathogen interaction, and their contribution](https://reader034.fdocuments.us/reader034/viewer/2022042322/5f0bfc2c7e708231d43330d0/html5/thumbnails/8.jpg)
Eigenvalues0
.00
.51
.01
.52
.02
.5 Multiple colinearities between quantitative traits
IE
SPS
SPL
LP
LS
PC1
PC2
![Page 9: Quantitative traits of host-pathogen interaction, and ... › fileadmin › 11_Henriette_Goyeau_pp.pdf · Quantitative traits of host-pathogen interaction, and their contribution](https://reader034.fdocuments.us/reader034/viewer/2022042322/5f0bfc2c7e708231d43330d0/html5/thumbnails/9.jpg)
Relationships between quantitative traits and field severity
IE
Date 1 Date 2 Date 3
All the quantitative traits were correlated with field disease severity, except for SPL.
P=1 P=1 P=1
Field
Disease
severity
![Page 10: Quantitative traits of host-pathogen interaction, and ... › fileadmin › 11_Henriette_Goyeau_pp.pdf · Quantitative traits of host-pathogen interaction, and their contribution](https://reader034.fdocuments.us/reader034/viewer/2022042322/5f0bfc2c7e708231d43330d0/html5/thumbnails/10.jpg)
Multiple Linear Regression
Disease severity at date i ~ PC1 * PC2
PC1 = axis 1 PCA (LP, LS, SPS and IE)
PC2 = axis 2 PCA (SPL)
Variable P value PC1 P value PC2
P value PC1*PC2
R2
Severity date 1
0.00029 ns ns 0.64
Severity date 2
0.00007 ns ns 0.72
Severity date 3
0.00005 ns ns 0.73
Infection Efficiency Latent Period Lesion Size Sporulation per unit area
Field disease severity correlated to
Multiple colinearities between quantitative traits and field severity
![Page 11: Quantitative traits of host-pathogen interaction, and ... › fileadmin › 11_Henriette_Goyeau_pp.pdf · Quantitative traits of host-pathogen interaction, and their contribution](https://reader034.fdocuments.us/reader034/viewer/2022042322/5f0bfc2c7e708231d43330d0/html5/thumbnails/11.jpg)
• Most of the quantitative traits were related (>0 or trade-off) [pleiotropic QTLs, trade-off]
• Field disease severity was correlated to quantitative traits assessed in controlled conditions [all the QTLs expressed in the glasshouse were expressed in the field]
• Assessment of quantitative traits in glasshouse experiments: – Different isolates => assessment of specificity
– Identification of diversified sources of resistance, to be combined
=> Durable Resistance
Conclusions
(Azzimonti et al., Plant Pathology, 2013)
![Page 12: Quantitative traits of host-pathogen interaction, and ... › fileadmin › 11_Henriette_Goyeau_pp.pdf · Quantitative traits of host-pathogen interaction, and their contribution](https://reader034.fdocuments.us/reader034/viewer/2022042322/5f0bfc2c7e708231d43330d0/html5/thumbnails/12.jpg)
Nicolas Lecutier
Anne-C. Zippert
Bénédicte Pariaud
Lucette Duveau
Thank you to …