Quantitative Analysis of Tannins in Plant

Extracts used as Diabetes Adjuvants

Wanda M. Figueroa-CuilanJannette Gavillán-Suárez, Ph.D.

Aknowledgements

Dr. Jannette Gavillán-Suárez

UPR Cayey’s Phytochemical and Pharmacognocy Research Lab

Sr. Juan Carlos Rodriguez

Syzygium Jambos Plant Provider

Tannins y diabetes

• They were reported to possess multiple biological activities including anticancer, antidiabetes, antioxidant and antimicrobial activities.

• Tannins are polyphenolic compounds found in foods such as legumes, vegetables, fruits, and beverages.

Previous WorkJournal: “The American Society for Nutritional Sciences”

TA is an Example of an antidiabetic Tannin

Qualitative Analysis

Objective

• Quantify the tannin content in plant extracts from Tapeinochiilus ananassae, Syzygium jambos, Costus speciosus, and Tradescantia spathacea.

• To correlate the scavenging and antioxidant activity of plant aqueous extracts with the quantity of tannins.

Hypothesis

• The Tannins content, will explain the scavenging and antioxidant activity.

• The more quantity of tannin the more

antioxidant the plant will it be.

Relevance of this Research

• This is the first time that the scavenging and antioxidant activity of the plant extracts studied in our research group is correlated with the Tannin content.

• “Most antidiabetic drugs promote weight gain” (Lui and Kim, 2005). Therefore, if the Tannin content is the cause of the scavenging and antioxidant activities, then may be useful to develop a new type of adjuvants for diabetes Type II and hyperglicemia.

Methodology: Calibration Curve

The calibration curve will be done

in 5 concentrations of

Tannic Acid:

•75µg/mL•100µg/m•125µg/mL•150µg/mL•175µg/mL

Add 500µL:0.1M FeCl38.0E-03 M K4Fe(CN)6

*3H2O

Spectrophotometer at 395nm

GraphAbs395Vs. [Tannic

Acid µg/mL]Beer Lambert Law

Methodology: Plant Test

Wash Grind leaves

Weight 1.0g

Add 50mL H2O Beaker 250mL Add grinded leaves

1hr/25C

Filtration

Filtrate

Pipette6.5mL

triplicado

Add 500µL:0.1M FeCl38.0E-03 M K4Fe(CN)6

*3H2O

Spectrophotometer at 395nm

Methodology: Plant Test

GraphAbs395Vs. [Tannic

Acid µg/mL]Beer Lambert Law

The abs is proportional to

the concentration

Results

Tapeinochilus Ananassae

Plant Abs395 Average: 1.23

x= 196.49µg/mL

Syzygium jambos

Plant Abs395 Average: 0.3790

x= 67.22µg/mL

Costus speciosus

Plant Abs395 Average: 0.0647

x= 30.32µg/mL

Tradescantia spathacea

Plant Abs395 Average:

0.183

x= 27.9µg/mL

Histogram Comparison Analysis

Plant Con of Tannins (µg/mL) ± SD

T.spathacea 27.95±0.22

C.speciosus 30.32±0.29

S.jambos 67.22±0.22

T.annassae 196.4±0.34

Tannin Content Scavenging Activity IC50

Plant IC50

T.spathacea 1132.57±275.77

C.speciosus 822.2±93.51

S.jambos 37.65±15.51

T.annassae 188.02±66.21

Future Work

• In the future a quantitative analysis of Terpenoids must be performed with the goal of test the synergy between the phytochemicals.

Summary

• Qualitative Tannin content results showed a correlation with the qualitative phenolic analysis.

• There was no correlation found between the superoxide radical scavenging and antioxidant activity and the Tannin content.

• The hypothesis that involve a particular phytochemical vs. a biological activity has to be rejected.

• We strongly believe in the synergy principle. The interaction of the phytochemicals in the plant extracts makes them show the antiabetic activities.

References

• Xueqing Liu, Jae-kyung Kim, Yunsheng Li, Jing Li, Fang Liu, and Xiaozhuo Chen. Tannic Acid Stimulates Glucose Transport and Inhibits Adipocyte Differentiation in 3T3-L1 Cells, J. Nutr. 2005 135: 2 165-171

• Kaur, L.; Han, K.S.; Bains, K.; Singh, H. Indian culinary plants enhance glucose-induced insulin secretion and glucose consumption in INS-1 b-cells and 3T3-L1 adipocytes. Food Chem. [Online] 2011, 29, 1120-1125. Science Direct. http://www. sciencedirect.com

• Aslan, M.; Deliorman Orhan, D.; Orhan, N.; Sezik, E.; Yesilada, E. In vivo antidiabetic and antioxidant potential of H elichrysum plicatumssp. plicatum capitulums in streptozotocin-induced- diabetic rats. J. Ethnopharmacol. [Online] 2007, 109, 54-59. Science Direct. http://www. sciencedirect.com

Quantitative Analysis of Tannins in Plant

Extracts used as Diabetes Adjuvants

Wanda M. Figueroa-CuilanJannette Gavillán-Suárez, Ph.D.