Quantification and Spatial Relationship Karsten Rodenacker, Neuherberg Martina Hausner, München...
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![Page 1: Quantification and Spatial Relationship Karsten Rodenacker, Neuherberg Martina Hausner, München Anna A. Gorbushina, Oldenburg Forschungszentrum g.](https://reader035.fdocuments.us/reader035/viewer/2022062511/551b71fe550346ae7a8b637e/html5/thumbnails/1.jpg)
Quantification and Spatial Relationship
Karsten Rodenacker,
NeuherbergMartina Hausner, München
Anna A. Gorbushina, Oldenburg
Forschungszentrumg
![Page 2: Quantification and Spatial Relationship Karsten Rodenacker, Neuherberg Martina Hausner, München Anna A. Gorbushina, Oldenburg Forschungszentrum g.](https://reader035.fdocuments.us/reader035/viewer/2022062511/551b71fe550346ae7a8b637e/html5/thumbnails/2.jpg)
Content Introduction
from perception to image analysis Measurement
objects, groups of objectsinter- and intra-relationships
Examples Conclusion
![Page 3: Quantification and Spatial Relationship Karsten Rodenacker, Neuherberg Martina Hausner, München Anna A. Gorbushina, Oldenburg Forschungszentrum g.](https://reader035.fdocuments.us/reader035/viewer/2022062511/551b71fe550346ae7a8b637e/html5/thumbnails/3.jpg)
Introduction Perception – recognition –
differentiation – description Quantification Relation of qualitative and
quantitative terms
![Page 4: Quantification and Spatial Relationship Karsten Rodenacker, Neuherberg Martina Hausner, München Anna A. Gorbushina, Oldenburg Forschungszentrum g.](https://reader035.fdocuments.us/reader035/viewer/2022062511/551b71fe550346ae7a8b637e/html5/thumbnails/4.jpg)
Introduction The difficulty NOT
to see something
![Page 5: Quantification and Spatial Relationship Karsten Rodenacker, Neuherberg Martina Hausner, München Anna A. Gorbushina, Oldenburg Forschungszentrum g.](https://reader035.fdocuments.us/reader035/viewer/2022062511/551b71fe550346ae7a8b637e/html5/thumbnails/5.jpg)
Introduction The difficulty
to see anything
![Page 6: Quantification and Spatial Relationship Karsten Rodenacker, Neuherberg Martina Hausner, München Anna A. Gorbushina, Oldenburg Forschungszentrum g.](https://reader035.fdocuments.us/reader035/viewer/2022062511/551b71fe550346ae7a8b637e/html5/thumbnails/6.jpg)
Introduction The ease to see the impossible
![Page 7: Quantification and Spatial Relationship Karsten Rodenacker, Neuherberg Martina Hausner, München Anna A. Gorbushina, Oldenburg Forschungszentrum g.](https://reader035.fdocuments.us/reader035/viewer/2022062511/551b71fe550346ae7a8b637e/html5/thumbnails/7.jpg)
Introduction Quantitative terms
![Page 8: Quantification and Spatial Relationship Karsten Rodenacker, Neuherberg Martina Hausner, München Anna A. Gorbushina, Oldenburg Forschungszentrum g.](https://reader035.fdocuments.us/reader035/viewer/2022062511/551b71fe550346ae7a8b637e/html5/thumbnails/8.jpg)
Introduction How to relate
qualitative and quantitative terms?
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Introduction Digitisation Segmentation
![Page 10: Quantification and Spatial Relationship Karsten Rodenacker, Neuherberg Martina Hausner, München Anna A. Gorbushina, Oldenburg Forschungszentrum g.](https://reader035.fdocuments.us/reader035/viewer/2022062511/551b71fe550346ae7a8b637e/html5/thumbnails/10.jpg)
Introduction Sub sectioning and change of scale
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Introduction
Extension Size Shape Structure
Measurement (of one object)
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Introduction
Arrangement Relation Neighbourhoo
d
Measurement (of several objects)
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Examples of measurements,objects and groups of objects
Spatial relationships
Measurement
![Page 14: Quantification and Spatial Relationship Karsten Rodenacker, Neuherberg Martina Hausner, München Anna A. Gorbushina, Oldenburg Forschungszentrum g.](https://reader035.fdocuments.us/reader035/viewer/2022062511/551b71fe550346ae7a8b637e/html5/thumbnails/14.jpg)
Measurement Area Perimeter Extension
![Page 15: Quantification and Spatial Relationship Karsten Rodenacker, Neuherberg Martina Hausner, München Anna A. Gorbushina, Oldenburg Forschungszentrum g.](https://reader035.fdocuments.us/reader035/viewer/2022062511/551b71fe550346ae7a8b637e/html5/thumbnails/15.jpg)
Measurement Shape Growth shape Density,
intensity
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Measurement Extensions
Length (skeleton)=1621 pxmean thickness=2.27 px
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Measurement Neighbourhood
closing onfilaments
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Measurement Spatial
RelationshipDelaunay triangulationnearest neighbours
minimum spanning treeconvex hull
Skeleton neighbourhood
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Measurement Spatial
RelationshipExample frompathology
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Measurement Spatial relationship (objects of different
type)
Distances to the red phaseDouble marked sludge flocks
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Measurement Measurement
continuum Measurement
hierarchy
pixel contentlocation properties
object
object
object
object
objectcontent
locationexternal properties
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Example Bacterial growth in flow chambers
Differentiation of wild and mutant bacteria pseudomonas aeruginosa by CLSM imaging
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Example Wild (PA) and
mutant (MW) bacteria
Growth overtime (slice # = depth)
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Example Substrate coverage (closing)
Wild type bacteria Mutant bacteria
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Example Bacterial growth in flow chambers
Conjugativegenetic transfer in bacterialbiofilm
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Example Quantification of colonies of micro
colonial fungi from sub aerial biofilms coniosporium sp. and sarcinomyces sp. under soil (b), sand (s) coverage and in air (l)
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Example Colonies of micro colonial fungi
![Page 28: Quantification and Spatial Relationship Karsten Rodenacker, Neuherberg Martina Hausner, München Anna A. Gorbushina, Oldenburg Forschungszentrum g.](https://reader035.fdocuments.us/reader035/viewer/2022062511/551b71fe550346ae7a8b637e/html5/thumbnails/28.jpg)
Example Colonies of micro colonial fungi
![Page 29: Quantification and Spatial Relationship Karsten Rodenacker, Neuherberg Martina Hausner, München Anna A. Gorbushina, Oldenburg Forschungszentrum g.](https://reader035.fdocuments.us/reader035/viewer/2022062511/551b71fe550346ae7a8b637e/html5/thumbnails/29.jpg)
Conclusion Perception, description
and measurement of objects and object groups in images
Exclusions (e.g. texture, filtering, fractals, etc.)
Faith and (apparent) truth