Quality Control of Culture media Dr Mohammad Rahbar Iranian Reference Health Laboratory.
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Transcript of Quality Control of Culture media Dr Mohammad Rahbar Iranian Reference Health Laboratory.
Quality Control of Culture media
Dr Mohammad RahbarIranian Reference Health Laboratory
Anaerobic sheep blood and laked blood agar
• Incubation Atmosphere Anaerobic• Length, and Temperature• 24-48 h, 35 °C• Control Organisms(ATCC ) Expected Reults• B. fragilis (25285) :Growth• C. perfringens (13124) : Growth beta
hemolysis• F. nucleatum (25586) : Growth• P. anaerobius (27337): Growth• P. melaninogenica (25845) : Growth
Blood agar—nonselective sheep blood agarmedia
• Incubation Atmosphere,• Aerobic or CO2,• Length, and Temperature; 18-24 h , 35 °C• Control Organisms (ATCC® No.) Expected Results
• S. pyogenes (19615) : Growth beta hemolysis• S. pneumoniae (6305) : Growth alpha hemolysis• S. aureus (25923) : growth• E. coli (25922) : Growth
Blood agar-CAMP test (trypticase soy agar[TSA] with sheep blood only)
• Incubation Atmosphere :Anaerobi• Length, and Temperature :24-48 h, 35 °C• Control Organisms ATCC• S. aureus (33862) or (25923):Growth• S. agalactiae (12386) :Positive reaction
(Arrowhead area of clearing)• S. pyogenes (19615) :Negative reaction (No
arrowhead formation)
Blood agar—Selective sheep blood agar media
(Columbia [CNA] agar,
• Incubation Atmosphere : CO2°• Length, and Temperature :24-48 h, 35 °C• Control Organisms : Expected Results• S. pyogenes (19615) : Growth mbetahemolyric• S. pneumoniae (6305) : Growth alpha
hemolytic• S. aureus (25923) Growth• P. mirabilis (12453) : Growth
Blood agar—Selective sheep blood agar media
(Columbia [CNA] agar,
• Incubation Atmosphere : CO2°• Length, and Temperature :24-48 h, 35 °C• Control Organisms : Expected Results• S. pyogenes (19615) : Growth mbetahemolyric• S. aureus (25923) Growth• P. mirabilis (12453): Inhibition (partial)
Blood culture media.(TSI and BHI)
• Incubation Atmosphere : Anaerobic and aerobic
• Length, and Temperature : 5 days, 35 °C• Control Organisms : Expected Results• B. fragilis (25285): Growth• S. pneumoniae (6305) : Growth
Campylobacter agar (user quality control
required)
• Incubation Atmosphere :Reduced O2 with CO2,
• Length, and Temperature : 48 h, 42 °C• Control Organisms : Expected Results
• C. jejuni (33291) : Growth• E. coli (25922): Inhibition (partial)
Chocolate agar
• Incubation Atmosphere : CO2• Length, and Temperature :24-48 h, 35 °C• Control Organisms ATCC• N. gonorrhoeae (43069) : Growth• H. influenzae (10211) : gROWTH
Cefsulodin irgasan novobiocin (CIN)agar
• Incubation Atmosphere : Aerobic• Length, and Temperature : 24-48 h, 25 oC• Control Organisms ATCC:• Y. enterocolitica (9610) :Growth; deep red center transparent
border (bull’seye)
• E. coli (25922) :Inhibition (partial to complete)
• P. aeruginosa (27853) Inhibition (partial to complete)
• E. faecalis (29212) Inhibition (partial to complete)
•
Buffered charcoal yeast extract(BCYE) (CYE/BCYE) agar
• Incubation Atmosphere,:Aerobic• Length, and Temperature :48-72 h, 35 °C• Control Organisms• L. pneumophila (33152) :Growth; yellow-green
fluorescence under long-wave• UV light• L. bozemanii (33217) :Growth; blue-white
fluorescence• L. micdadei (33204 :Growth
Enrichment broths for enterics(gram-negative [GN] broth, selenite
broths)
• Incubation Atmosphere,:Aerobic• Length, and Temperature :18-24h, 35 °C• Control Organisms• S. typhimurium (14028) : Growth on subculture• S. sonnei (9290) : Growth on subculture (may be
inhibited by selenite• media)• E. coli (25922): Inhibition (partial to complete) on
subculture Growth on subculture from GN broth
Eosin methylene blue media(Levine EMB agar; EMB agar,
modified)
• Incubation Atmosphere,: Aerobic• Length, and Temperature : 18-24 h, 35 °C• Control Organisms:• S. typhimurium (14028) : Growth, colorless to
amber colonies• E. coli (25922): Growth, blue-black colonies with
green metallic• sheen• E. faecalis (29212): Inhibition (partial)
Hektoen enteric (HEK) agar
• Incubation Atmosphere, :Aerobic• Length, and Temperature :18-24 h, 35 °C• Control Organism:• S. typhimurium (14028): Growth, colonies blue to
green-blue with black• centers• S. flexneri (12022) :Growth, colonies green to blue-
green• E. faecalis (29212):Inhibition (partial); colonies yellow• E. coli (25922) :Inhibition (partial to complete);
colonies yellow to salmon colored
MacConkey agar
• Incubation Atmosphere, :Aerobic• Length, and Temperature :18-24 h, 35 °C• Control Organism
E. coli (25922) : Growth, pink colonies• P. mirabilis (12453) : Growth, colorless colonies,
partial inhibition of swarming• S. typhimurium (14028): Growth, colorless
colonies• E. faecalis (29212) : Inhibition (partial)
Mannitol salt agar
• Incubation Atmosphere, :Aerobic• Length, and Temperature :24-48h, 35 °C• Control Organism;• S. aureus (25923) : Growth, colonies have
yellow zones at 48 h• S. epidermidis (12228): Growth, colonies have
red zones at 48 h• P. mirabilis (12453) : Inhibition (partial)
Salmonella-Shigella (SS) agar
• Incubation Atmosphere, :Aerobic• Length, and Temperature :24 h, 35 °C• Control Organism• S. typhimurium (14028) : Growth, colonies
colorless with or without black centers• S. flexneri (12022) : Growth, colorless colonies• E. faecalis (29212) : Inhibition (complete)• E. coli (25922) : Inhibition (partial to complete;
colonies pink to rose-red with precipitate)
Selective media for pathogenic Neisseria spp.(TM)
• Incubation Atmosphere, :Co2• Length, and Temperature :24 -48h, 35 °C• Control Organism:• N. gonorrhoeae (43069) :Growth• N. meningitidis (13090) :Growth• P. mirabilis (43071) :Inhibition (partial) use only for media• containing trimethoprim• E. coli (25922) :Inhibition (partial)• N. sicca (9913) :Inhibition (partial)• C. albicans (60193) :Inhibition (partial)• S. epidermidis (12228) :Inhibition (partial)
Selective media for enterococci, with azide
• Incubation Atmosphere, :Aerobic• Length, and Temperature :24 -48h, 35 °C• Control Organism:• E. faecalis (29212) :Growth, blackening around
colonies• S. pyogenes (19615):Inhibition (partial to
complete)• E. coli (25922):Inhibition (partial) - Colorless
colonies on bile esculin agar
Selective media for enterococci, without azide
• Incubation Atmosphere, :Aerobic• Length, and Temperature :24 -48h, 35 °C• Control Organism:• E. faecalis (29212) :Growth, blackening
around colonies• S. pyogenes (19615):Inhibition (partial to
complete)
Thioglycolate broth, with or without indicator
• Incubation Atmosphere, :Aerobic, 48 h (tightened , cap
• Length, and Temperature : 48h, 35 °C• Control Organism:• B. fragilis (25285) : Growth• S. aureus (25923): Growth
Thioglycolate broth, enriched with vitamin Kand hemin
• Incubation Atmosphere, :Aerobic, 48 h (tightened , cap
• Length, and Temperature : 48h, 35 °C• Control Organism:• P. anaerobius (27337) : Growth• B. vulgatus (8482): Growth• C. perfringens (13124) : Growth
Tubed media (brain heart infusion and tryptic
soy broth)
• Incubation Atmosphere, :Aerobic• Length, and Temperature :18-24, 35 °C• Control Organism:• E. coli (25922) : Growth• S. aureus (25923) : Growth
Xylose lysine desoxycholate (XLD) agar
• Incubation Atmosphere, :Aerobic• Length, and Temperature : 24, 35 °C• Control Organism:• S. typhimurium (14028) :Growth - colonies red
with black centers• S. flexneri (12022) :Growth - colonies red• E. faecalis (29212) :Inhibition partial• E. coli (25922) :Inhibition (partial to complete;
colonies yellow to yellow-red)