Quality Control: Molecular Diagnostics
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Transcript of Quality Control: Molecular Diagnostics
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Quality Control: Molecular Diagnostics
Molecular Diagnostics Quality Control - Module 8 22
Overview Sample Collection, Transport, and Processing
maintain Nucleic acid integrity through these processes
Contamination Control establish a unidirectional work flow from a DNA-
free area for reagent preparation, to a sample processing area, to area where amplification and detection occur
Positive and Negative Controls Reference: CLSI MM3-A (Molecular Diagnostic
Methods for Infectious Diseases)
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Quality Control: Molecular Diagnostics
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Real Time PCRSAMPLE
PRIMER
Taq polymerase Amplified Target NA
SYBR GreenQuantitative Fluorescence
+ - In
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Amplification Reactions
Amplification / Time
POSITIVE
NO AMPLIFICATION
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Real Time RT-PCR
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A/H3
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A/H5
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Risks with PCR most RT-PCR is still done as “in-house”
or “home-brew”primers are not necessarily optimized to
testpositive controls are “in-house”negative controls are “in-house”
interpretation of tests need to be done with caution