Putting the puzzle together: Connecting the HyperSAS with BreveBuster absorbance spectra,...
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![Page 1: Putting the puzzle together: Connecting the HyperSAS with BreveBuster absorbance spectra, fluorometer, extracted chlorophyll and MODIS data. Blake A. Schaeffer,](https://reader035.fdocuments.us/reader035/viewer/2022070401/56649f1b5503460f94c3085b/html5/thumbnails/1.jpg)
Putting the puzzle together: Connecting the HyperSAS with BreveBuster absorbance spectra, fluorometer, extracted chlorophyll and MODIS data. Blake A. Schaeffer, D.
Kamykowski, J. M. Morrison, S. Banks, A. McCulloch, and W. V. Sweet
![Page 2: Putting the puzzle together: Connecting the HyperSAS with BreveBuster absorbance spectra, fluorometer, extracted chlorophyll and MODIS data. Blake A. Schaeffer,](https://reader035.fdocuments.us/reader035/viewer/2022070401/56649f1b5503460f94c3085b/html5/thumbnails/2.jpg)
![Page 3: Putting the puzzle together: Connecting the HyperSAS with BreveBuster absorbance spectra, fluorometer, extracted chlorophyll and MODIS data. Blake A. Schaeffer,](https://reader035.fdocuments.us/reader035/viewer/2022070401/56649f1b5503460f94c3085b/html5/thumbnails/3.jpg)
Introduction
• The Galapagos harbors such great diversity because of its positioning in a complex transition zone between tropical, subtropical and upwelled waters.
• Only place in the world where hammerhead sharks, penguins, and sealions co-exist on the same sub-tidal reef.
![Page 4: Putting the puzzle together: Connecting the HyperSAS with BreveBuster absorbance spectra, fluorometer, extracted chlorophyll and MODIS data. Blake A. Schaeffer,](https://reader035.fdocuments.us/reader035/viewer/2022070401/56649f1b5503460f94c3085b/html5/thumbnails/4.jpg)
Introduction
• Three major current systems influence the Galapagos.
• Cool southern currents from the Humboldt (Peru) system influencing the southern islands.
• Warmer Panama currents that influence the northern islands.
• Strong, large-scale Equatorial Undercurrent (EUC) upwelling in the western archipelago.
![Page 5: Putting the puzzle together: Connecting the HyperSAS with BreveBuster absorbance spectra, fluorometer, extracted chlorophyll and MODIS data. Blake A. Schaeffer,](https://reader035.fdocuments.us/reader035/viewer/2022070401/56649f1b5503460f94c3085b/html5/thumbnails/5.jpg)
Focus
• The EUC is the major oceanographic feature affecting production in the Galápagos, and is strongly associated with local biogeography of marine species.
• Galápagos biogeography/marine ecosystem heterogeneity is a function of oceanographic patterning over small spatial, seasonal and inter-annual scales.
![Page 6: Putting the puzzle together: Connecting the HyperSAS with BreveBuster absorbance spectra, fluorometer, extracted chlorophyll and MODIS data. Blake A. Schaeffer,](https://reader035.fdocuments.us/reader035/viewer/2022070401/56649f1b5503460f94c3085b/html5/thumbnails/6.jpg)
Goals
• Hyperspectral information combined with BreveBuster absorbance spectra, fluorometer, extracted chlorophyll and MODIS data will identify variability of phytoplankton biomass throughout the Galapagos.
• Hyperspectral information will elucidate phytoplankton taxonomy of the Galapagos with the support of HPLC pigment analysis, particulate absorption spectra and BreveBuster data.
![Page 7: Putting the puzzle together: Connecting the HyperSAS with BreveBuster absorbance spectra, fluorometer, extracted chlorophyll and MODIS data. Blake A. Schaeffer,](https://reader035.fdocuments.us/reader035/viewer/2022070401/56649f1b5503460f94c3085b/html5/thumbnails/7.jpg)
Methods
• Sierra Negra• 70 hydrographic
stations• 12 days
![Page 8: Putting the puzzle together: Connecting the HyperSAS with BreveBuster absorbance spectra, fluorometer, extracted chlorophyll and MODIS data. Blake A. Schaeffer,](https://reader035.fdocuments.us/reader035/viewer/2022070401/56649f1b5503460f94c3085b/html5/thumbnails/8.jpg)
Methods
• Phytoplankton – 20 m net– 20 meters depth
• Filtration– Surface only– Extracted chlorophyll a– Particulate absorption
spectra
• Seapoint FL– Top 2 meters bin average
Seapoint Fluorometer
Phytoplankton tows
Filtration
![Page 9: Putting the puzzle together: Connecting the HyperSAS with BreveBuster absorbance spectra, fluorometer, extracted chlorophyll and MODIS data. Blake A. Schaeffer,](https://reader035.fdocuments.us/reader035/viewer/2022070401/56649f1b5503460f94c3085b/html5/thumbnails/9.jpg)
HyperSAS
Methods• MODIS
– Chlorophyll a– SeaDAS v 4.9
• BreveBuster– In situ spectrometer– Phytoplankton class
spectral libraries– Day and night travel
• HyperSAS– 166-channel MiniSpec
radiance and irradiance sensors
– Day travel only
BreveBuster – Mote Marine Aquarium
![Page 10: Putting the puzzle together: Connecting the HyperSAS with BreveBuster absorbance spectra, fluorometer, extracted chlorophyll and MODIS data. Blake A. Schaeffer,](https://reader035.fdocuments.us/reader035/viewer/2022070401/56649f1b5503460f94c3085b/html5/thumbnails/10.jpg)
March 2005Chlorophyll a
(g/L)
084 089
Wet season
Panama current
dominates.
![Page 11: Putting the puzzle together: Connecting the HyperSAS with BreveBuster absorbance spectra, fluorometer, extracted chlorophyll and MODIS data. Blake A. Schaeffer,](https://reader035.fdocuments.us/reader035/viewer/2022070401/56649f1b5503460f94c3085b/html5/thumbnails/11.jpg)
November 2005Chlorophyll a
(g/L)
324 347
Transitionfrom garua
(dry) to wet
season.
Panama current replaces
Peru current.
![Page 12: Putting the puzzle together: Connecting the HyperSAS with BreveBuster absorbance spectra, fluorometer, extracted chlorophyll and MODIS data. Blake A. Schaeffer,](https://reader035.fdocuments.us/reader035/viewer/2022070401/56649f1b5503460f94c3085b/html5/thumbnails/12.jpg)
Transition from wet to garua
(dry).
June 2006Chlorophyll a
(g/L)
190181
![Page 13: Putting the puzzle together: Connecting the HyperSAS with BreveBuster absorbance spectra, fluorometer, extracted chlorophyll and MODIS data. Blake A. Schaeffer,](https://reader035.fdocuments.us/reader035/viewer/2022070401/56649f1b5503460f94c3085b/html5/thumbnails/13.jpg)
MODIS chlorophyll a shows similar trends and range to Seapoint FL chlorophyll a.
MODIS chlorophyll a show similartrends to extracted chlorophyll a, but less agreement in range.
HyperSAS identifies variability not present in MODIS chlorophyll a.
![Page 14: Putting the puzzle together: Connecting the HyperSAS with BreveBuster absorbance spectra, fluorometer, extracted chlorophyll and MODIS data. Blake A. Schaeffer,](https://reader035.fdocuments.us/reader035/viewer/2022070401/56649f1b5503460f94c3085b/html5/thumbnails/14.jpg)
Remote sensing reflectance
![Page 15: Putting the puzzle together: Connecting the HyperSAS with BreveBuster absorbance spectra, fluorometer, extracted chlorophyll and MODIS data. Blake A. Schaeffer,](https://reader035.fdocuments.us/reader035/viewer/2022070401/56649f1b5503460f94c3085b/html5/thumbnails/15.jpg)
Chlo
rophyll
a
Phyco
cyanin
Chlo
rophyll
a
Caro
tenoid
s
Particulate absorption
![Page 16: Putting the puzzle together: Connecting the HyperSAS with BreveBuster absorbance spectra, fluorometer, extracted chlorophyll and MODIS data. Blake A. Schaeffer,](https://reader035.fdocuments.us/reader035/viewer/2022070401/56649f1b5503460f94c3085b/html5/thumbnails/16.jpg)
Particulate absorption spectra
![Page 17: Putting the puzzle together: Connecting the HyperSAS with BreveBuster absorbance spectra, fluorometer, extracted chlorophyll and MODIS data. Blake A. Schaeffer,](https://reader035.fdocuments.us/reader035/viewer/2022070401/56649f1b5503460f94c3085b/html5/thumbnails/17.jpg)
BreveBuster Phytoplankton Class ID
![Page 18: Putting the puzzle together: Connecting the HyperSAS with BreveBuster absorbance spectra, fluorometer, extracted chlorophyll and MODIS data. Blake A. Schaeffer,](https://reader035.fdocuments.us/reader035/viewer/2022070401/56649f1b5503460f94c3085b/html5/thumbnails/18.jpg)
BreveBuster Phytoplankton Class ID
![Page 19: Putting the puzzle together: Connecting the HyperSAS with BreveBuster absorbance spectra, fluorometer, extracted chlorophyll and MODIS data. Blake A. Schaeffer,](https://reader035.fdocuments.us/reader035/viewer/2022070401/56649f1b5503460f94c3085b/html5/thumbnails/19.jpg)
Phytoplankton Tows
![Page 20: Putting the puzzle together: Connecting the HyperSAS with BreveBuster absorbance spectra, fluorometer, extracted chlorophyll and MODIS data. Blake A. Schaeffer,](https://reader035.fdocuments.us/reader035/viewer/2022070401/56649f1b5503460f94c3085b/html5/thumbnails/20.jpg)
Future work• HPLC pigment analysis
– Chemtax
• Particulate absorption spectral library
• Remote sensing reflectance 4th derivative analysis
• HyperSAS phytoplankton class spectral library
• Poster: 4.28-P Today 12:00 – Seasonality of
phytoplankton distributions in the Galapagos Marine Reserve
Chlo
rophyll a
?
Caro
tenoid
s?
Chlo
rophyll a
?
![Page 21: Putting the puzzle together: Connecting the HyperSAS with BreveBuster absorbance spectra, fluorometer, extracted chlorophyll and MODIS data. Blake A. Schaeffer,](https://reader035.fdocuments.us/reader035/viewer/2022070401/56649f1b5503460f94c3085b/html5/thumbnails/21.jpg)
Thank you!
Questions?