Proximate analysis, antimicrobial and antioxidant activities of aloe vera.

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Presented By, ASHIADEY SELASI KOJO Supervised by, DR. NATHANIEL OWUSU BOADI 1

Transcript of Proximate analysis, antimicrobial and antioxidant activities of aloe vera.

Page 1: Proximate analysis, antimicrobial and antioxidant activities of aloe vera.

Presented By,

ASHIADEY SELASI KOJO

Supervised by,

DR. NATHANIEL OWUSU BOADI

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Page 2: Proximate analysis, antimicrobial and antioxidant activities of aloe vera.

OUTLINE INTRODUCTION

OBJECTIVES

METHODOLOGY

RESULTS AND DISCUSSION

CONCLUSION

REFERENCES

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INTRODUCTIONProximate analysis is the quantitative analysis of

food to determine the percentage of fat, fibre, protein,

carbohydrate, moisture and ash. (Merriam Webster

Dictionary)

An antimicrobial is an agent that kills

microorganisms or inhibits their growth. (Merriam

Webster Dictionary)

An antioxidant is a molecule capable of inhibiting

the oxidation of other molecules. (Sies, 1997) Eg

gallic acid and ferulic acid3

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INTRODUCTION

ALOE VERA

What is aloe vera?

Where does it originate from?

Parts of aloe vera leaf

Healing properties and general uses

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INTRODUCTION

SOME HEALTH BENEFITS OF ALOE VERA It is a skin moisturizer

Lowers high blood pressure

Lowers high cholesterol

Stabilizes blood sugar

Acts as a detoxifier

Lubricates the digestive tract

(Mike Adams, 2007)

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AIMS AND OBJECTIVES To estimate the determination of fat, fibre, protein,

moisture, carbohydrate and ash in aloe vera.

To determine the antimicrobial effect of aloe vera

extract using the agar well diffusion method.

To determine the antioxidant activity of aloe vera

extract using the DPPH free radical scavenging

method.

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METHODOLOGY

EXTRACTION OF THE ALOE VERA GEL

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1 2 3

4 5

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METHODOLOGY

Proximate analysis of aloe vera MOISTURE

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%MOISTURE by

oven drying method

%PROTEIN by

Kjeldahl method

%N x 6.25

DIGESTION

using H2SO4

DISTILLATION

of mixture with

CONC NaOH

TITRATION

with 0.1N HCl%LIPIDS by soxhlet

method

%FIBRE by acid and

alkaline digestion of

sample with 1.25%

H2SO4 and 1.25%

NaOH

%ASH by dry

ashing method

%CARBOHYDRATE

by 100 - % ash,

%fibre, %protein and

% fat

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METHODOLOGY

AGAR WELL DIFFUSION METHOD

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INJECT

BACTERIA

INTO AGAR

PLATE, PURE

NUTRIENT

AGAR

CREAT WELLS IN

AGAR PIPETTE

EXTRACT IN

WELLS +

CONTROL

INCUBATION

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METHODOLOGYANTIOXIDANT ACTIVITY OF ALOE VERA

8 serial dilutions of the extract and ascorbic acid(control)

each were prepared

2ml of DPPH reagent was added to each of the serial

dilutions

They were incubated in the dark for 30 minutes

Absorbance was measured at 517nm

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Results for proximate analysisNUTRIENTS RESULTS(Amount of nutrients in %)

Carbohydrate 0.68%

Moisture 99.2%

Ash 0.02%

Fibre 0.06%

Fat 0.01%

Protein 0.027%

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RESULTS FOR ANTIMICROBIAL ANALYSIS

(ZONE OF INHIBITIONS IN MM)

Organisms 20mg/ml 15mg/ml 13mg/ml 10mg/ml

Staphylococcus

aureus

- - - -

Streptococcus

pyogenes

- - - -

Escherichia

Coli

19mm - - -

Pseudomonas

aeroginosa

- - - -

Candida

albicans

4mm - - -

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RESULTS FOR ANTIOXIDANT ACTIVITY

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0

10

20

30

40

50

60

70

0.08 0.04 0.02 0.01 0.005 0.002 0.001 0.0006

% i

nh

ibit

ion

Concentration in mg/ml

A bar chart of % inhibition against the concentration of the extract in mg/ml

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CONCLUSION The Proximate analysis of aloe vera gel shows a high level

of carbohydrate and moisture with a little bit of protein,

mineral content and fat. Therefore it can be suitable for

human consumption.

Aloe vera has antimicrobial activity and can be used to

heal some diseases.

Aloe vera has antioxidant activity and can be essential to

human health.

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REFERENCES Mohammad, A. (2003). Aloe Vera. Textbook of

Pharmacognosy. 111-115.

Atherton, P. (1998), ‘Aloe vera: magic or medicine?’, Nursing Standard, July 1- 7, 41, 12.

Park Y, Lee S(2006). New Perspectives on Aloe. New York: Springer Verlag

Payne, J.M. (1970). Tissue response to Aloe vera gel following periodontal surgery. Master's thesis. (accessed October 5, 2009)

Adams, M. (2005). The aloe vera miracle: A natural medicine for cancer, cholesterol, diabetes, inflammation, IBS and other health conditions.

Sies, H. (1997). "Oxidative stress: Oxidants and antioxidants". Experimental physiology 82 (2): 291–5

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THANK

YOU16