Protein-DNA Interactions · Transcription of DNA sequence into RNA sequence RNA 1 RNA 2 RNA 3...

28
Protein-DNA Interactions

Transcript of Protein-DNA Interactions · Transcription of DNA sequence into RNA sequence RNA 1 RNA 2 RNA 3...

Page 1: Protein-DNA Interactions · Transcription of DNA sequence into RNA sequence RNA 1 RNA 2 RNA 3 Translation (on the ribosome) of RNA sequence into protein sequence and folding of protein

Protein-DNA Interactions

Page 2: Protein-DNA Interactions · Transcription of DNA sequence into RNA sequence RNA 1 RNA 2 RNA 3 Translation (on the ribosome) of RNA sequence into protein sequence and folding of protein

The central dogma of biology

Page 3: Protein-DNA Interactions · Transcription of DNA sequence into RNA sequence RNA 1 RNA 2 RNA 3 Translation (on the ribosome) of RNA sequence into protein sequence and folding of protein

Genomic DNA

Page 4: Protein-DNA Interactions · Transcription of DNA sequence into RNA sequence RNA 1 RNA 2 RNA 3 Translation (on the ribosome) of RNA sequence into protein sequence and folding of protein

DNA packaging

Page 5: Protein-DNA Interactions · Transcription of DNA sequence into RNA sequence RNA 1 RNA 2 RNA 3 Translation (on the ribosome) of RNA sequence into protein sequence and folding of protein

DNA replication

Page 6: Protein-DNA Interactions · Transcription of DNA sequence into RNA sequence RNA 1 RNA 2 RNA 3 Translation (on the ribosome) of RNA sequence into protein sequence and folding of protein

DNA replication

Page 7: Protein-DNA Interactions · Transcription of DNA sequence into RNA sequence RNA 1 RNA 2 RNA 3 Translation (on the ribosome) of RNA sequence into protein sequence and folding of protein

The genetic code

Page 8: Protein-DNA Interactions · Transcription of DNA sequence into RNA sequence RNA 1 RNA 2 RNA 3 Translation (on the ribosome) of RNA sequence into protein sequence and folding of protein

Transcription of DNA

Page 9: Protein-DNA Interactions · Transcription of DNA sequence into RNA sequence RNA 1 RNA 2 RNA 3 Translation (on the ribosome) of RNA sequence into protein sequence and folding of protein

Translation of mRNA - Protein synthesis

Page 10: Protein-DNA Interactions · Transcription of DNA sequence into RNA sequence RNA 1 RNA 2 RNA 3 Translation (on the ribosome) of RNA sequence into protein sequence and folding of protein

DNA structure

Page 11: Protein-DNA Interactions · Transcription of DNA sequence into RNA sequence RNA 1 RNA 2 RNA 3 Translation (on the ribosome) of RNA sequence into protein sequence and folding of protein

DNA structure

Page 12: Protein-DNA Interactions · Transcription of DNA sequence into RNA sequence RNA 1 RNA 2 RNA 3 Translation (on the ribosome) of RNA sequence into protein sequence and folding of protein

DNA structure

Page 13: Protein-DNA Interactions · Transcription of DNA sequence into RNA sequence RNA 1 RNA 2 RNA 3 Translation (on the ribosome) of RNA sequence into protein sequence and folding of protein

Protein-DNA interactionsMinor & Major grooves- Recognition patterns

Page 14: Protein-DNA Interactions · Transcription of DNA sequence into RNA sequence RNA 1 RNA 2 RNA 3 Translation (on the ribosome) of RNA sequence into protein sequence and folding of protein

Protein-DNA interactionsMinor & Major grooves- Recognition patterns

Page 15: Protein-DNA Interactions · Transcription of DNA sequence into RNA sequence RNA 1 RNA 2 RNA 3 Translation (on the ribosome) of RNA sequence into protein sequence and folding of protein

Protein-DNA interactionsMinor & Major grooves- Recognition patterns

Page 16: Protein-DNA Interactions · Transcription of DNA sequence into RNA sequence RNA 1 RNA 2 RNA 3 Translation (on the ribosome) of RNA sequence into protein sequence and folding of protein

Protein-DNA interactionsSequence specific vs. non-specific recognition

Page 17: Protein-DNA Interactions · Transcription of DNA sequence into RNA sequence RNA 1 RNA 2 RNA 3 Translation (on the ribosome) of RNA sequence into protein sequence and folding of protein

The RNA polymerase

Page 18: Protein-DNA Interactions · Transcription of DNA sequence into RNA sequence RNA 1 RNA 2 RNA 3 Translation (on the ribosome) of RNA sequence into protein sequence and folding of protein

The RNA polymerase

Page 19: Protein-DNA Interactions · Transcription of DNA sequence into RNA sequence RNA 1 RNA 2 RNA 3 Translation (on the ribosome) of RNA sequence into protein sequence and folding of protein

The RNA polymeraseRNA synthesis begins at promoter sites

Page 20: Protein-DNA Interactions · Transcription of DNA sequence into RNA sequence RNA 1 RNA 2 RNA 3 Translation (on the ribosome) of RNA sequence into protein sequence and folding of protein

The RNA polymeraseRNA synthesis begins at promoter sites

Page 21: Protein-DNA Interactions · Transcription of DNA sequence into RNA sequence RNA 1 RNA 2 RNA 3 Translation (on the ribosome) of RNA sequence into protein sequence and folding of protein

Gene regulationNegative regulation

Page 22: Protein-DNA Interactions · Transcription of DNA sequence into RNA sequence RNA 1 RNA 2 RNA 3 Translation (on the ribosome) of RNA sequence into protein sequence and folding of protein

Gene regulationNegative regulation

Lactose repressor

Page 23: Protein-DNA Interactions · Transcription of DNA sequence into RNA sequence RNA 1 RNA 2 RNA 3 Translation (on the ribosome) of RNA sequence into protein sequence and folding of protein

Gene regulationNegative regulation

Lactose repressor

Page 24: Protein-DNA Interactions · Transcription of DNA sequence into RNA sequence RNA 1 RNA 2 RNA 3 Translation (on the ribosome) of RNA sequence into protein sequence and folding of protein

Gene regulationPositive regulation

Page 25: Protein-DNA Interactions · Transcription of DNA sequence into RNA sequence RNA 1 RNA 2 RNA 3 Translation (on the ribosome) of RNA sequence into protein sequence and folding of protein

Gene regulationPositive regulation

cAMP Receptor Protein (CRP)

Page 26: Protein-DNA Interactions · Transcription of DNA sequence into RNA sequence RNA 1 RNA 2 RNA 3 Translation (on the ribosome) of RNA sequence into protein sequence and folding of protein

Gene regulationPositive regulation

Page 27: Protein-DNA Interactions · Transcription of DNA sequence into RNA sequence RNA 1 RNA 2 RNA 3 Translation (on the ribosome) of RNA sequence into protein sequence and folding of protein

Gene regulationPositive regulation

Page 28: Protein-DNA Interactions · Transcription of DNA sequence into RNA sequence RNA 1 RNA 2 RNA 3 Translation (on the ribosome) of RNA sequence into protein sequence and folding of protein

Gene regulationPositive regulation


RNA & Protein Synthesis

RNA & Protein Synthesis

. Class 2: Basic Sequence Alignment. Sequence Comparison Much of bioinformatics involves sequences u DNA sequences u RNA sequences u Protein sequences.

. Class 2: Basic Sequence Alignment. Sequence Comparison Much of bioinformatics involves sequences u DNA sequences u RNA sequences u Protein sequences.

Computer Aided Drug Design ——Protein structure predictioncbb.sjtu.edu.cn/~qinxu/files/lecture3.pdf · 2014. 9. 24. · prediction ? • Sequence – From DNA/RNA sequence –

Computer Aided Drug Design ——Protein structure predictioncbb.sjtu.edu.cn/~qinxu/files/lecture3.pdf · 2014. 9. 24. · prediction ? • Sequence – From DNA/RNA sequence –

TRANSLATION: information transfer from RNA to protein the nucleotide sequence of the mRNA strand is translated into an amino acid sequence. This is accomplished.

TRANSLATION: information transfer from RNA to protein the nucleotide sequence of the mRNA strand is translated into an amino acid sequence. This is accomplished.

DNA => RNA => Protein

DNA => RNA => Protein

Rna protein-synthesis

Rna protein-synthesis

RNA Sequence Assembly

RNA Sequence Assembly

Molecular Basis for Relationship between Genotype and Phenotype DNA RNA protein genotype function organism phenotype DNA sequence amino acid sequence transcription.

Molecular Basis for Relationship between Genotype and Phenotype DNA RNA protein genotype function organism phenotype DNA sequence amino acid sequence transcription.

Sequence Alignment. 2 Sequence Comparison Much of bioinformatics involves sequences u DNA sequences u RNA sequences u Protein sequences We can think of.

Sequence Alignment. 2 Sequence Comparison Much of bioinformatics involves sequences u DNA sequences u RNA sequences u Protein sequences We can think of.

RNA and Transcription DNA RNA PROTEIN. RNA and Transcription.

RNA and Transcription DNA RNA PROTEIN. RNA and Transcription.

12-3 The Structure of RNA The Central Dogma21-30Key).pdf · 12-3 RNA and Protein Synthesis 12-3 The Structure of RNA A gene is a SEQUENCE of DNA that codes for a protein (or functional

12-3 The Structure of RNA The Central Dogma21-30Key).pdf · 12-3 RNA and Protein Synthesis 12-3 The Structure of RNA A gene is a SEQUENCE of DNA that codes for a protein (or functional

RNA Sequence

RNA Sequence

18. The Reference Sequence (RefSeq) Project€¦ · The Reference Sequence (RefSeq) database provides a biologically non-redundant collection of DNA, RNA, and protein sequences. Each

18. The Reference Sequence (RefSeq) Project€¦ · The Reference Sequence (RefSeq) database provides a biologically non-redundant collection of DNA, RNA, and protein sequences. Each

Example Of Dna Sequence To Rna Sequence

Example Of Dna Sequence To Rna Sequence

RNA multiple sequence alignment

RNA multiple sequence alignment

CSE182-L16 - University of California, San Diego · CSE182-L16 Non-coding RNA. Biol. Data analysis: Review Protein Sequence Analysis Sequence Analysis Gene Finding ... •Abstract

CSE182-L16 - University of California, San Diego · CSE182-L16 Non-coding RNA. Biol. Data analysis: Review Protein Sequence Analysis Sequence Analysis Gene Finding ... •Abstract

PROTEIN SYNTHESIS TRANSCRIPTION: DNA m RNA TRANSLATION: m RNA Protein.

PROTEIN SYNTHESIS TRANSCRIPTION: DNA m RNA TRANSLATION: m RNA Protein.

RNA PROCESSING DNA MATURE RNA PROTEIN PRECURSOR RNAs pre-rRNA pre-tRNA pre-mRNA Cleavage Nucleotide addition Nucleotide insertion Nucleotide removal Sequence.

RNA PROCESSING DNA MATURE RNA PROTEIN PRECURSOR RNAs pre-rRNA pre-tRNA pre-mRNA Cleavage Nucleotide addition Nucleotide insertion Nucleotide removal Sequence.

a) Sequence b) Sequence-structureresearch.cs.queensu.ca/home/cisc857/CISC875_old/Fortier.pdf · DNA, RNA, and protein sequences and ... h for protein structure motifs b egins with

a) Sequence b) Sequence-structureresearch.cs.queensu.ca/home/cisc857/CISC875_old/Fortier.pdf · DNA, RNA, and protein sequences and ... h for protein structure motifs b egins with

Database search. Overview ： 1. FastA ： is suitable for protein sequence searching 2. BLAST ： is suitable for DNA, RNA, protein sequence searching.

Database search. Overview ： 1. FastA ： is suitable for protein sequence searching 2. BLAST ： is suitable for DNA, RNA, protein sequence searching.