Protein Crystallisation Strategies, optimisation and non-standard methods.
-
Upload
marian-wilkins -
Category
Documents
-
view
221 -
download
0
Transcript of Protein Crystallisation Strategies, optimisation and non-standard methods.
Protein Crystallisation
Strategies, optimisation and non-standard
methods
Overview
First steps in crystallising proteinsAvailable screensScreening strategiesConsidering the leadsOptimisation experiment designsNon-standard methodsPractical methods
– Simple non-standard techniques– Microbatch gels
First steps in crystallising proteins
Finding crystallising conditions for your protein
– It is necessary to screen a broad range of conditions to determine the solubility of your protein
– At this stage insoluble protein is observed as amorphous precipitate
– In the second optimisation stage potential conditions are explored
Examination of plates
– Record the appearance of each well on a regular basis
– Observe immediately after setting up and again on the following day again after 2 or 3days and then once a week
– Use a stereomicroscope to make the observations
– Be careful not to shake or jolt plates when moving them
What you should look for
– Crystals are transparent and have definite form recognisable by the planar faces
– Precipitate is irregular in shape without defined edges, opaque and often forms clumps
– Phase separation in the form of bubbles which may be disperse or quite dense.
Identifying salt crystals
• Protein dye
• Crushing
• Dehydration
• Ultimately the x-ray beam
Overview
–First steps in crystallising proteins
–Available screens
–Screening strategies
–Considering the leads
–Optimisation experiment designs
–Non-standard methods
Available screens
• Principles behind screening – to determine solubility and for optimisation.
• Types of screen available– Sparse matrix screens– Clear strategy– PEG ion– Detergent screens– Additive screens
Screening strategies
Since it is impossible to predict the conditions for nucleation, screening is a good way of determining the crystallising conditions.Random screens
– Trial and error sparse matrix approach
Systematic screens– Selected variation of two parameters
Sparse matrix screens
• Sparse matrix screens are composed of a collection of conditions which have been used successfully for crystallisation of other proteins
• Within the screens the following parameters are varied: – pH, precipitating agent, type of buffer and
salt components
Overview
–First steps in crystallising proteins
–Available screens
–Screening strategies
–Interpreting results
–Optimisation experiment designs
–Non-standard methods
Interpreting results
Skills in crystallisation are:– Describing your observations - hampton score sheet– Interpreting the results of an experiment– Deciding what to do next
Identifying and following the leads– Precipitation - types of precipitate, granular,
microcrystalline– Phase separation - phase separation bubbles, gel
precipitate– Micro crystals - is it protein or salt?
What the leads might mean
Amorphous or granular precipitate– May/may not be the ideal crystallisation condition,
concentration of protein or precipitant too high
Phase separation and phase gel– If all observations are phase separation related
select those that have a gelatinous appearance
Microcrystalline precipitate and crystals– Likely to be the correct conditions concentration of
protein or precipitant too high
Using the leads to gain understanding
• Gathering information from the screens to gain an understanding of the solubility of your macromolecule– Make a note of the pH of the screen condition, are
there any trends regarding pH?– Any trends regarding salts? Difference in results
with high salt and low salt– Hofmeister series – ranking of ions in order of their
ability to precipitate proteins– Any common appearances e.g. lots of precipitate
or only phase separation
Hofmeister series
• Cations: Li+ > Na+>K+>NH+4 >Mg2+
• Anions: sulphate 2- > phosphate2- > acetate- > citrate3- > tartrate2- > bicarbonate- > chromate2- > chloride-
> nitrate- >> chlorate - > thiocyanate-
Optimisation experiment designs
After completing an initial screen you may have one of the following results:
1. crystals with one or more conditions2. amorphous precipitates or precrystalline
aggregates with one or more conditions3. no crystals, precipitate or aggregates with
any of the conditions in the screen
If you obtained results 1 or 2 you may want to fine tune your screen.
Fine tuning the sparse matrix conditions
• The sparse matrix screen has yielded a number of conditions in which your protein is insoluble (crystals or precipitate)
• Design a narrow-range grid screen based on varying the pH, and the concentrations of each component systematically observing whether one or more of the variations gives good crystals
Expanding the initial screen
It is possible that none of the conditions from your first screen gave any leads to expand your screening it is worth trying other sparse matrix screens which are commercially available or a grid screening kit
Exercise to practice optimisation skills
How would you go about optimising the crystallisation to achieve the following and why:
– larger crystals– fewer crystals– improve the diffraction quality
Overview
–First steps in crystallising proteins
–Available screens
–Screening strategies
–Interpreting results
–Optimisation experiment designs
–Non-standard methods
Non-standard methods
• Microbatch crystallisation with gels
• Microbatch controlled evaporation
• Oil barrier methods
• Containerless crystallisation
• Separation of nucleation and growth
Adapting the screens
Adapting the screens– Using dilution– Using evaporation– Using oils– Screen at a different temperature– Screen at a different pH– Using gels
Microbatch crystallisation
Drops between 3l and 0.3l are dispensed under oil either by hand or by robot under oil.
Chayen and Saridakis (2002) Acta Cryst. D58, 921-927
Controlled Evaporation
Evaporation methods can be applied to both microbatch and vapour diffusion methods. In the case of microbatch, the drops are dispensed under a thin layer of oil is to allow limited evaporation. After a predetermined time the tray is filled with oil to prevent any further evaporation.
Advantages of crystallisation in microbatch Under Oil
• Some crystals will ONLY grow in oil• Hanging drops tend to spread over the surface of
siliconised cover slips • Mechanically batch is the simplest crystallisation
method which lends itself readily to HTP• Very small drop volumes down to 1nl• Crystals can be grown under controlled nucleation
conditions in three ways by:i. choosing the oil which covers the trialsii. varying the thickness of the oil layer covering
the trialsiii. applying a ‘container-less’ crystallisation set-up
Problems Associated with Microbatch Crystallisation
• Shock nucleation• Use of organic components• Stabilising / harvesting crystals
Oil barrier methods
Method to control crystallisation by altering the rate of vaporisation from the reservoir and therefore the rate at which the drop equilibrates.
Methods which utilise separation of nucleation and growth
This is achieved by transferring the cover slip with the drop from a reservoir with crystallisation agent at higher concentration to one with
a reservoir at lower concentration.
Approaches to aid crystallisation
• Trial and error– Screening and fine tuning conditions for
crystal growth
• Systematic studies– Understanding the fundamental principles
of crystal growth– Designing experiments using these
principles to produce better crystals
The systematic approach
As an example of the screening process suppose you found that condition 35 of the Hampton crystal screen (0.1M HEPES, pH 7.5; 1.6M Na/K phosphate) gave amorphous precipitate, you might set up the following grid screen:
The systematic approach
pH 7.0 pH 7.2 pH 7.4 pH 7.6 pH 7.8 pH 8.0
Na/K 0.8
Na/K 0.8
Na/K 0.8
Na/K 0.8
Na/K 0.8
Na/K 0.8
Na/K 1.0
Na/K 1.0
Na/K 1.0
Na/K 1.0
Na/K 1.0
Na/K 1.0
Na/K 1.2
Na/K 1.2
Na/K 1.2
Na/K 1.2
Na/K 1.2
Na/K 1.2
Na/K 1.4
Na/K 1.4
Na/K 1.4
Na/K 1.4
Na/K 1.4
Na/K 1.4
Designing your own screens
Have a go and use your Intuition!• Screen with salt and PEG• Grid screen with buffered ammonium
sulphate• pH screen with one precipitant
A constant temperature
crystallization room.
Inside a crystallization roomSetting up crystallization trials
Looking at crystallization trialsMany conditions are screened
Optimization of Crystallization Conditions
Here are some good crystals.
In difficult cases it is typical to try homologous proteins from several different species.
The need to grow crystals is often the limiting step in structure determination. This is an empirical process that involves much trial and error. Commercial kits are used to sample hundreds of trial conditions. When encouraging leads are found (e.g. small crystals), the initial conditions are refined.
Good crystals will be about 0.1 - 0.5 mm in diameter, and have no flaws, such as cracks, or two crystals growing together.