ProTec® PRP:

Our system is a closed-system that is easily processed in 5 easy steps. It includes a 9mL blood draw and 6-minute centrifuge spin that results in ~5 mL of Pure PRP ready for sterile injection. Some of the unique benefits of our system:

- A separating gel that effectively results in no RBC contamination and minimal to no WBC traces - so literally you get the most out of Pure PRP and takes out the guesswork common with other systems - Chemically balanced mix of anti-coagulant (sodium citrate) and coagulant (calcium chloride) helps form a fibrin matrix that protects platelets and releases healing growth factors over 7 days (unlike other systems that release in just hours and are inconsistent with healing time) - Equine System: PRP processing can be done stall side!!! - Cost-effective: pays for itself Cost of the system is $2,250 (centrifuge, mobile centrifuge case w/ wheels, 5-kits and shipping) Cost for small animal vets/others not needing the mobile centrifuge case would be $1,680. Kits, thereafter, are sold in 5-packs and pricing is $995 plus $35 shipping (so $199 a kit)

For videos and more information, visit www.ProTecPRP.com

PROTEC® PRP PROCESSING – QUICK ACCESS TO ESSENTIAL STEPS

See complete Instructions for Use in Kit for more detailed

step-by-step visuals

STEP 1

Draw 9 mL of blood into yellow

tube using 21-gauge needle and

invert 7 times to mix with anti-

coagulant (sodium citrate)

STEP 2

Place yellow tube in centrifuge with red holders and utilize counter balance tube weight on opposite side.

Note: Yellow caps can be put on top of red holder to secure plasma tube

during spinning

Yellow start button will begin a 6-minute spin. If the patient is a dog, please note that blood may vary in certain dogs so an

additional spin for complete separation may be needed. Note: As timing is important, prep the animal during spinning so you are prepared

for final steps and to inject

Following centrifuge spin, invert the tube 7 times to optimize the

mix of platelets and plasma

STEP 3 - CRUCIAL STEP TO ENSURE VACUUM/TRANSFER

Remove the blue cap from vial and place vial on a flat surface.

Hold firmly in place with one hand. Using your dominant hand to

hold transfer device, evenly align and center the transfer device

to the top of the vial without the two touching or puncturing yet.

Then press device down with one forceful push (you will hear it

snap if it was aligned correctly). This will help ensure an adequate

vacuum for transfer.

STEP 4

Invert yellow PRP tube and place into plastic transfer

device where it will puncture so that PRP

can begin to transfer into the vial.

Following complete transfer of PRP, remove plasma tube

and invert the vial with transfer cap on

7 times to mix with coagulant (calcium chloride). Note: Plasma will begin to coagulate in 10-15 minutes following this inversion so

immediately prepare for injection

STEP 5

Screw off top portion of transfer device and attach

syringe. Invert vial and withdraw PRP. Twist the vial

off of the syringe and twist on 21-gauge needle to the

syringe, remove safety cap, and inject.

Get the most out of Pure PRPProTec ® PRP – Simple, consistent preparation meets effective healingPlatelet-rich plasma (PRP) involves getting the most out of viable platelets within plasma. These platelets thrive long enough to distribute healing growth factors to an injury to stimulate recovery.

Preparation that’s easy and consistent• Blood-separating technology ensures pure PRP – separating gel with anti-

coagulant (sodium citrate) physically separates plasma and platelets from red and white blood cells - takes the guess work out of PRP separation

• Only 9 mL of blood and 6-minute centrifuge spin to achieve consistent plasma and platelet separation every time

• Calcium chloride included in vial counterbalances anti-coagulant for effective formation of a fibrin matrix that protects platelets and healing growth factors

• Closed-system preparation results in 4-5 mL of PRP ready for sterile injection into tendon/ligaments, soft-tissue and wounds

ProTec PRP: pure, protected platelet technology – effective healing with minimal start-up costs

Delivering protected platelets• Simple and effective preparation results in platelets that are 95% viable and are not

prematurely activated prior to injection• Vital growth factors within platelets are protected until activation • Controlled release of healing growth factors is gradual – over 7

days1 – consistent with healing times• The protective fibrin matrix fills the void within an injury and

allows for healing cells to migrate, proliferate, and deposit tissue for remodeling

5-STEP

PRP

MADE EASY

Official PRP Supplier of the USEF

The Healing Power of Pure,Protected PlateletsOptimizing platelet activation and preservation

• Platelet concentration is based on the amount of initial blood drawn and the remaining plasma content when red and white blood cells are separated from it

• ProTec has a concentration of 2x (based on a hematocrit of 50%) – a sufficient concentration for beneficial healing

• 2x concentration does not compromise the additional benefits/proteins plasma may offer. Attempting to achieve higher concentrations by removing more plasma may be done so at the expense of other vital components

• Coagulant helps to optimize the platelets and form a fibrin matrix inside the body post-injection – so platelets remain at the injection site and growth factors release over time 1

• No added external proteins or thrombin necessary - Alternate coagulants may saturate platelets and result in a fast, premature

release of growth factors inconsistent with healing times - The addition of bovine thrombin has been shown to release the majority of the

growth factors in 8 hours. TGF beta (TGF-β – Transforming Growth Factor Beta) and bFGF (Basic Fibroblast Growth Factor), the most common growth factors linked to early wound repair, were almost fully released in just 2 hours 2

• To date, no studies have identified the “ideal” concentration level for greatest efficacy, as many factors in healing with PRP are patient dependent. However, it has been shown that an excessive concentration may inhibit cellular response and healing³

Portable PRP processing and economically smart • Portable, light-weight centrifuge in a ready-to-go case – adaptable for in-clinic or remote treatments• Minimal start-up costs: low-cost centrifuge and sterile kits

For more information, visit PureProTec.com1 Carroll RJ, et al. Characterization of autologous growth factors in Cascade platelet rich fibrin matrix (PRFM). Musculoskeletal Transplant Foundation. Edison, NJ 2005.2 Swift MJ, et al. Characterization of growth factors in platelet rich plasma. Proc. Ortho. Res. Soc. 2005, Wash D.C.3 Giusti I, et al. Identification of an optimal concentration of platelet gel for promoting angiogenesis in human endothelial cells. Transfusion. 2009;49:771-778.

©2013 Pulse Veterinary Technologies, LLC

PlateletsGrowth Factors

Fibrin

Tissue / Collagen

Optimizing Platelet Release

Fibrinogen in plasma forms fibrin fibrin is released following injection and adheres to collagen fibers in an

overlapping matrix formation growth factors released from platelets

are held in matrix and gradually released to stimulate healing