PROTEC GILL - SkrettingProtec Gill 3 weeks feeding Protec Gill 6 weeks feeding Protec Gill Before...

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PROTEC GILL GILLS: INCREASES NON-SPECIFIC IMMUNE RESPONSE REINFORCES THE IMMUNE SYSTEM FAST RECOVERY FROM GILL INJURY ADDITIONAL ATTRIBUTES OF PROTEC GILL PROTEC ATTRIBUTES IMPROVES INTESTINAL HEALTH STABILISES AND PROMOTES INTESTINAL MICROFLORA STRENGTHENS SKIN AND SCALES SUPPORTS THE CRITICAL PROCESSES OF THE IMMUNE SYSTEM INCREASES ANTIOXIDANT CAPACITY IMPROVES VACCINE EFFICACY REDUCES PATHOGEN LOADING IN FISH IMPROVES GILL FUNCTION

Transcript of PROTEC GILL - SkrettingProtec Gill 3 weeks feeding Protec Gill 6 weeks feeding Protec Gill Before...

PROTEC GILL

GILLS: INCREASES NON-SPECIFIC

IMMUNE RESPONSE

REINFORCES THE IMMUNE

SYSTEM

FAST RECOVERY FROM GILL INJURY

ADDITIONAL ATTRIBUTES OF PROTEC GILL

PROTEC

ATTRIBUTES

IMPROVES INTESTINAL HEALTH

STABILISES AND PROMOTES INTESTINAL

MICROFLORA

STRENGTHENS SKIN AND SCALES

SUPPORTS THE CRITICAL PROCESSES OF THE IMMUNE SYSTEM

INCREASES ANTIOXIDANT

CAPACITY

IMPROVES VACCINEEFFICACY

REDUCES PATHOGEN

LOADING IN FISH

IMPROVES GILL FUNCTION

The

info

rmat

ion

pres

ente

d in

thi

s do

cum

ent

orig

inat

es f

rom

fiel

d tr

ials

und

erta

ken

by S

kret

ting

Chi

le i

nto

the

role

nut

riti

on c

an p

lay

in t

he m

anag

emen

t of

gill

hea

lth.

Protec Protec Gill Control

Lyso

zym

es in

mu

cu

s (

U/m

l)

1000

900

800

700

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0Protec Gill

component 1 Protec Gill

component 2 Negative

control

Gro

wth

pe

rce

nta

ge

97

96

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87

EFFECTS OF THE COMPONENTS OF PROTEC GILL ON THE GROWTH OF AMOEBA

FEEDING PROTEC GILL FACILITATES THE RAPID RECOVERY FROM GILL INJURY

Me

dia

n g

ill s

co

re

Control

Treated group fed control diet

Treated group fed Protec Gill

Control

Treated group fed control diet

Treated group fed Protec Gill

0.50

1.00

1.50

2.00

PROTEC GILL INCREASES ANTI-BACTERIAL POTENTIAL OF GILL MUCUS

FISH FED PROTEC GILL HAVE IMPROVED GILL IMMUNITY

Diet Control

0.0

0.5

1.0

1.5

Re

lati

ve

exp

ressio

n o

f g

en

es c

om

pa

red

to

th

e

co

ntr

ol g

rou

p a

t e

ac

h s

am

plin

g p

oin

t

Inmunoglobulin T

Lysozyme

MACROSCOPIC GILL SCORE

Score 0

Photos- Richard Taylor, CSIRO

Ref: Taylor, R.S., Muller, W.J., Cook, M.T.,Kube, P.D., Elliott, N.G., 2009. Aquaculture 290, 1-8.

Infection Level Gill Score Gross Description

0 No sign of infection and healthy red colour

Very Light 1 1 white spot, light scarring or undefined necrotic streaking

2 2-3 spots/small mucus patch

Moderate 3 Attached thickened mucus patch or spot groupings up to 20% of gill area

Advanced 4 Lesions covering up to 50% of gill area

5 Extensive lesions covering most of the gill surface

GILL SCORE CARD PREPARED SAMPLES

Score 1

Score 2

Score 1 Score 2 Score 3

GILL SCORE CARD PREPARED SAMPLES

, P.D., Elliott, N.G., 2009. Aquaculture 290, 1-8.

Gross Description

No sign of infection and healthy red colour

1 white spot, light scarring or undefined necrotic streaking

2-3 spots/small mucus patch

Attached thickened mucus patch or spot groupings up to 20% of gill area

Lesions covering up to 50% of gill area

Extensive lesions covering most of the gill surface

Score 1 Score 2 Score 3 Score 4 Score 5

GILL SCORE CARD PREPARED SAMPLES

Score 3

Score 4

Score 5

RESOLUTION OF INJURIES IN FISH FED PROTEC GILL

1.30

0.80

0.30H

isto

sc

ore

Before using Protec Gill

3 weeks feedingProtec Gill

6 weeks feedingProtec Gill Before using

Protec Gill3 weeks feeding

Protec Gill6 weeks feeding

Protec Gill

Before using Protec Gill

3 weeks feedingProtec Gill

6 weeks feedingProtec Gill

Comparison of the relative expression of the genes involved in the innate or non-specific immune response

and the specific or humoral immune response (IgT) in fish fed Protec Gill versus a control diet

**

*

*

*** P. perurans amoeba

exposed to the mucus of

Protec Gill fed salmon;

differences measured

after 72 hours

The presence of the

cytokine lysozyme in

the mucus indicates a

local immune

response and a higher

anti-bacterial potential

Histo score

classification is a

semiquantitative

measurement of gill

damage

A better quality

mucus generates a

more effective barrier

to gill pathogens

Protec Protec Gill Control

2.5

2.0

1.5

1.0

0.5

0.0

******

PROTEC GILL INCREASES THE VISCOSITY OF GILL MUCUS

42% RELATIVE PERCENTAGE SURVIVAL (RPS) COMPARED TO CONTROL

FED FISH FOLLOWING AGD CHALLENGE TRIALS

Protec Gill increases GILL CAPACITY

WHEN TO USE PROTEC GILL?

RECOMMENDATION FOR PROTEC GILL USE: 6 WEEKS

• Before and During gill health challenges

Feeding Protec Gill 6 weeks ahead of known risk periods and/or at the commencement of gill disease outbreaks

until its resolution will provide additional mitigation against the effects of the challenge

• Before and After Bath Treatments

Feeding Protec Gill for 3 weeks before and 3 weeks after Hydrogen Peroxide (H2O2) treatments will support the

regeneration of gill structure and function

The

info

rmat

ion

pres

ente

d in

thi

s do

cum

ent

orig

inat

es f

rom

fiel

d tr

ials

und

erta

ken

by S

kret

ting

Chi

le i

nto

the

role

nut

riti

on c

an p

lay

in t

he m

anag

emen

t of

gill

hea

lth.

**

***

FISH FED PROTEC GILL HAD A

PROTEC GILL FED FISH ARE BETTER ABLE TO REGULATE BLOOD SODIUM AND AMMONIUM LEVELS INDICATING AN IMPROVED OSMORAGULATORY ABILITY

Asterisks signify statistically significant differences

between diets

Asterisks signify statistically significant differences

between diets

Asterisks signify statistically significant differences

between diets

Vis

cosi

ty o

f m

ucus

(lysozyme)