PROTEC GILL - SkrettingProtec Gill 3 weeks feeding Protec Gill 6 weeks feeding Protec Gill Before...
Transcript of PROTEC GILL - SkrettingProtec Gill 3 weeks feeding Protec Gill 6 weeks feeding Protec Gill Before...
PROTEC GILL
GILLS: INCREASES NON-SPECIFIC
IMMUNE RESPONSE
REINFORCES THE IMMUNE
SYSTEM
FAST RECOVERY FROM GILL INJURY
ADDITIONAL ATTRIBUTES OF PROTEC GILL
PROTEC
ATTRIBUTES
IMPROVES INTESTINAL HEALTH
STABILISES AND PROMOTES INTESTINAL
MICROFLORA
STRENGTHENS SKIN AND SCALES
SUPPORTS THE CRITICAL PROCESSES OF THE IMMUNE SYSTEM
INCREASES ANTIOXIDANT
CAPACITY
IMPROVES VACCINEEFFICACY
REDUCES PATHOGEN
LOADING IN FISH
IMPROVES GILL FUNCTION
The
info
rmat
ion
pres
ente
d in
thi
s do
cum
ent
orig
inat
es f
rom
fiel
d tr
ials
und
erta
ken
by S
kret
ting
Chi
le i
nto
the
role
nut
riti
on c
an p
lay
in t
he m
anag
emen
t of
gill
hea
lth.
Protec Protec Gill Control
Lyso
zym
es in
mu
cu
s (
U/m
l)
1000
900
800
700
600
500
400
300
200
100
0Protec Gill
component 1 Protec Gill
component 2 Negative
control
Gro
wth
pe
rce
nta
ge
97
96
95
94
93
92
91
90
89
88
87
EFFECTS OF THE COMPONENTS OF PROTEC GILL ON THE GROWTH OF AMOEBA
FEEDING PROTEC GILL FACILITATES THE RAPID RECOVERY FROM GILL INJURY
Me
dia
n g
ill s
co
re
Control
Treated group fed control diet
Treated group fed Protec Gill
Control
Treated group fed control diet
Treated group fed Protec Gill
0.50
1.00
1.50
2.00
PROTEC GILL INCREASES ANTI-BACTERIAL POTENTIAL OF GILL MUCUS
FISH FED PROTEC GILL HAVE IMPROVED GILL IMMUNITY
Diet Control
0.0
0.5
1.0
1.5
Re
lati
ve
exp
ressio
n o
f g
en
es c
om
pa
red
to
th
e
co
ntr
ol g
rou
p a
t e
ac
h s
am
plin
g p
oin
t
Inmunoglobulin T
Lysozyme
MACROSCOPIC GILL SCORE
Score 0
Photos- Richard Taylor, CSIRO
Ref: Taylor, R.S., Muller, W.J., Cook, M.T.,Kube, P.D., Elliott, N.G., 2009. Aquaculture 290, 1-8.
Infection Level Gill Score Gross Description
0 No sign of infection and healthy red colour
Very Light 1 1 white spot, light scarring or undefined necrotic streaking
2 2-3 spots/small mucus patch
Moderate 3 Attached thickened mucus patch or spot groupings up to 20% of gill area
Advanced 4 Lesions covering up to 50% of gill area
5 Extensive lesions covering most of the gill surface
GILL SCORE CARD PREPARED SAMPLES
Score 1
Score 2
Score 1 Score 2 Score 3
GILL SCORE CARD PREPARED SAMPLES
, P.D., Elliott, N.G., 2009. Aquaculture 290, 1-8.
Gross Description
No sign of infection and healthy red colour
1 white spot, light scarring or undefined necrotic streaking
2-3 spots/small mucus patch
Attached thickened mucus patch or spot groupings up to 20% of gill area
Lesions covering up to 50% of gill area
Extensive lesions covering most of the gill surface
Score 1 Score 2 Score 3 Score 4 Score 5
GILL SCORE CARD PREPARED SAMPLES
Score 3
Score 4
Score 5
RESOLUTION OF INJURIES IN FISH FED PROTEC GILL
1.30
0.80
0.30H
isto
sc
ore
Before using Protec Gill
3 weeks feedingProtec Gill
6 weeks feedingProtec Gill Before using
Protec Gill3 weeks feeding
Protec Gill6 weeks feeding
Protec Gill
Before using Protec Gill
3 weeks feedingProtec Gill
6 weeks feedingProtec Gill
Comparison of the relative expression of the genes involved in the innate or non-specific immune response
and the specific or humoral immune response (IgT) in fish fed Protec Gill versus a control diet
**
*
*
*** P. perurans amoeba
exposed to the mucus of
Protec Gill fed salmon;
differences measured
after 72 hours
The presence of the
cytokine lysozyme in
the mucus indicates a
local immune
response and a higher
anti-bacterial potential
Histo score
classification is a
semiquantitative
measurement of gill
damage
A better quality
mucus generates a
more effective barrier
to gill pathogens
Protec Protec Gill Control
2.5
2.0
1.5
1.0
0.5
0.0
******
PROTEC GILL INCREASES THE VISCOSITY OF GILL MUCUS
42% RELATIVE PERCENTAGE SURVIVAL (RPS) COMPARED TO CONTROL
FED FISH FOLLOWING AGD CHALLENGE TRIALS
Protec Gill increases GILL CAPACITY
WHEN TO USE PROTEC GILL?
RECOMMENDATION FOR PROTEC GILL USE: 6 WEEKS
• Before and During gill health challenges
Feeding Protec Gill 6 weeks ahead of known risk periods and/or at the commencement of gill disease outbreaks
until its resolution will provide additional mitigation against the effects of the challenge
• Before and After Bath Treatments
Feeding Protec Gill for 3 weeks before and 3 weeks after Hydrogen Peroxide (H2O2) treatments will support the
regeneration of gill structure and function
The
info
rmat
ion
pres
ente
d in
thi
s do
cum
ent
orig
inat
es f
rom
fiel
d tr
ials
und
erta
ken
by S
kret
ting
Chi
le i
nto
the
role
nut
riti
on c
an p
lay
in t
he m
anag
emen
t of
gill
hea
lth.
**
***
FISH FED PROTEC GILL HAD A
PROTEC GILL FED FISH ARE BETTER ABLE TO REGULATE BLOOD SODIUM AND AMMONIUM LEVELS INDICATING AN IMPROVED OSMORAGULATORY ABILITY
Asterisks signify statistically significant differences
between diets
Asterisks signify statistically significant differences
between diets
Asterisks signify statistically significant differences
between diets
Vis
cosi
ty o
f m
ucus
(lysozyme)