ProStar 325 Detector Petro van Poppel – Field Support Specialist Friday, 9 October 2015.
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Transcript of ProStar 325 Detector Petro van Poppel – Field Support Specialist Friday, 9 October 2015.
![Page 1: ProStar 325 Detector Petro van Poppel – Field Support Specialist Friday, 9 October 2015.](https://reader031.fdocuments.us/reader031/viewer/2022020320/56649ea15503460f94ba464f/html5/thumbnails/1.jpg)
ProStar 325 Detector
Petro van Poppel – Field Support Specialist Friday, 21 April 2023
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Overview
Introduction to PS 325 Detector
• Dual Wavelength Option• LC Verify Software• Flow cells• CommunicationsLC Verify
• Production Tests with 0x0 flow cell• Wavescan Test (Mono Options)• Diagnostics Mode (Main Tab)• Electronics Test (Input/Output)• System Details (EEPROM)• Communications (COMM)PS 335 Shipping Test
Problems
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325 Optics Module Light Path
D2
Vis
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325 Optics Ray Beam
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Dual Wavelength Option
Dual wavelength is a firmware change that allows you to view 2 wavelengths “simultaneously”
Galaxie Software
LC Diagnostics Software
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325/335 Back Panel
I/O adaptor board for the sync signals
P/N 02-101865-00
P/N 02-101875-00
Relay Adaptor board
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Flow Cell Ray Beam
General design of the 9x0 flow cell General design of the 9x1 flow cell
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Flow Cell Specs
Flow Cell Prep
(9 x 1)
Super Prep
(4 x 0.15)
Analytical
(9 x 0)
Micro
(4 x 0 )
Pressure rating
1000 psi 1000 psi 1000 psi 1000 psi
Flow Cell Volume
30 µl 145 µl 15 µl 1.5 µl
Material (non optical)
Ti & PEEK Ti & PEEK Ti & PEEK Ti & PEEK
Noise Performance
200 ml/min
10-500ml
1x10-4AU
800 ml/min
0.80-10 L
1x10-4AU
40 ml/min
0.1 µl-100 ml
5x10-3AU
1 ml/min
0-20 ml
7.5x10-3AU
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Communications
TCP/IP communications for Detector:
•Static or Dynamic
•Networked or standalone
•Firewall
•Access on Network, OS or PC
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LC Verify side
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LC Diagnostics Side
Ctrl-Shift-F10 to enable Diagnostics sideCtrl-Shift-F9 to hide Diagnostics side
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PS 325 Test Certificate
Test Certificate provided by factory
Parameters tested:1) Noise2) Wavelength Accuracy3) Wavelength Reproducibility4) Spectral Bandwidth
Accuracy5) AutoZero Precision Test6) Detector Linearity Test
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LC Verify use in Final Testing
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PS 325 Noise Test (FT)
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PS 325 Wavelength Accuracy Test (FT)
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PS 325 Wavelength Repeatability Test (FT)
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PS 325 Spectral Bandwidth Test (FT)
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PS 325 Auto Precision Test (FT)
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PS 325 Detector Linearity Test (FT)
pump water for at least 10 minutes at a pump head pressure of at least 100 atm using a back pressure restrictor of 40psi.
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PS 325 LCVerify Wavescan
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PS 325 Wavescan results
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PS 325 Contributions from each Lamp
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PS 325 LCVerify Diagnostics Mode
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PS 325 LCVerify Input/Output Test
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PS 325 Main PWB Power Supply LED’s
A: + 24 V power SupplyB: + 9V and – 9V Power SupplyC: + 5 V Power Supply
BA
C
Front of the Unit
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PS 325 LCVerify System Details
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PS 325 LCVerify Communications
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Caffeine Shipping Test
PS 325 Caffeine Shipping Test:
Test made up of 1 components:
• Loop injection using 25 µg/ml caffeine
Test is conducted using Galaxie Software
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PS 325 Lamp Intensity Test
Test 1: Lamp Intensities
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PS 325/335 Caffeine Loop Injection Test
Test Calculates:Peak HeightPeak AreaNoiseS/N
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Common Problems
•High Noise
•Lamp on, not ready
•SIG_CC_ZERO_ORDER_PEAK_FIND_FAILED
•SIG_CC_SIGNAL_OVERRANGE_REPREFORM_ LAMP_CAL
•COM_CC_CIRCULAR_BUFFER_OVERRUN _OCCURED
•9555 LMP_CC_D2_FILAMENT_FAILURE
•PROSTAR 325 DRIVER: Collection not started - Acquisition time too short for the method.
•Foggy Optics
PS 325 Error Messages Table can be found:
• PS 325 Service Manual• Dealernet
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Foggy Optics
What is Foggy Optics?
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What is Foggy Optics?
Three types of Foggy optics
• White powdery coating
• Rust/brown spot burn
• Speckled mirror (delamination)
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Foggy Optics
What is Foggy Optics?
Diagnosing Foggy Optics
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Diagnosing Foggy Optics
Run a “wavescan” test,
When bad
Open the optics and inspect.
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Wavescan test results
Intensity for the 254 nm lineCurrently is 10 – 50 K counts
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False positive
New D2 vs Aged D2 Intensities
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Foggy Optics
What is Foggy Optics?
Diagnosing Foggy Optics
Treating Foggy optics
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0603 Project (Foggy Optics)
Proposed Fixes and their status:
White powder fogging: due to leaky flow cells and customer sample depositing on the mirrors
New Flow cells & fittings available for all cells except 4 x 0.15
Brown powder fogging: due to UV attack on some of the foam parts close to the UV source.
Eliminating as much of the foam as possible and shielding the rest.
Mirror delamination: due to UV attack on the thin Al coating of the mirrors
Eliminated by putting a NiCr coating on the mirrors to strengthen the Al coating.
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Opening and cleaning the optics
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Cleaning 325 optics
Clean area in circle
Do not adjust!!
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Replacing Optics
Wavelength calibration data must be entered into the 335 EEPROM
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Summary
•Single and Dual Wavelength, UV and UV/vis
•Optical path is open
•Flow cell is dual path
•Diagnostics Tests, like wavescan
•Shipping Test
•Main Problems (Foggy Optics)
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