Proposal writing. What is your question Why is your study important How are you going to do it Key...
-
Upload
rodger-walker -
Category
Documents
-
view
214 -
download
0
Transcript of Proposal writing. What is your question Why is your study important How are you going to do it Key...
![Page 1: Proposal writing. What is your question Why is your study important How are you going to do it Key Points of Your Proposal Who’s the boss.](https://reader035.fdocuments.us/reader035/viewer/2022062519/5697bfe91a28abf838cb6cfd/html5/thumbnails/1.jpg)
Proposal writing
![Page 2: Proposal writing. What is your question Why is your study important How are you going to do it Key Points of Your Proposal Who’s the boss.](https://reader035.fdocuments.us/reader035/viewer/2022062519/5697bfe91a28abf838cb6cfd/html5/thumbnails/2.jpg)
What is your question
Why is your study important
How are you going to do it
Key Points of Your Proposal
Who’s the boss
![Page 3: Proposal writing. What is your question Why is your study important How are you going to do it Key Points of Your Proposal Who’s the boss.](https://reader035.fdocuments.us/reader035/viewer/2022062519/5697bfe91a28abf838cb6cfd/html5/thumbnails/3.jpg)
![Page 4: Proposal writing. What is your question Why is your study important How are you going to do it Key Points of Your Proposal Who’s the boss.](https://reader035.fdocuments.us/reader035/viewer/2022062519/5697bfe91a28abf838cb6cfd/html5/thumbnails/4.jpg)
1. You can write in either English or Chinese for this proposal. However, both Chinese and English titles and abstracts are required.
2. For terms not universally known, spell out the term the first time it is used followed by the appropriate abbreviation in parentheses; the abbreviation may be used thereafter.
3. Type the proposal in single spaced, 12 point, Times New Roman.
4. You must include at least one figure following the rules instructed in the class
General guideline
![Page 5: Proposal writing. What is your question Why is your study important How are you going to do it Key Points of Your Proposal Who’s the boss.](https://reader035.fdocuments.us/reader035/viewer/2022062519/5697bfe91a28abf838cb6cfd/html5/thumbnails/5.jpg)
5. The Research Plan of your project should consist of in the order of all the following components:
Chinese and English title and abstracts Hypothesis and Specific aims Background and significance Preliminary data Research design and methods Anticipated results References.
The maximum number of pages is 10 pages including reference.
![Page 6: Proposal writing. What is your question Why is your study important How are you going to do it Key Points of Your Proposal Who’s the boss.](https://reader035.fdocuments.us/reader035/viewer/2022062519/5697bfe91a28abf838cb6cfd/html5/thumbnails/6.jpg)
Title and Abstracts (1 page maximum)
name &lab中文標題
摘要
English titleAbstract
name &lab中文標題
摘要
English titleAbstract
10-1
Put both Chinese and English titles and abstracts in the first page of your proposal.
Describe concisely the research design and methods for achieving these goals (250 words maximum).
![Page 7: Proposal writing. What is your question Why is your study important How are you going to do it Key Points of Your Proposal Who’s the boss.](https://reader035.fdocuments.us/reader035/viewer/2022062519/5697bfe91a28abf838cb6cfd/html5/thumbnails/7.jpg)
Title
• Title must reflect the central theme: selling point
• Attractive and easy to understand• Concise and Informative: avoid non-informative
words like “ A study of---”• Avoid acronyms• English and Chinese titles should be consistent
but not word-to-word translation
![Page 8: Proposal writing. What is your question Why is your study important How are you going to do it Key Points of Your Proposal Who’s the boss.](https://reader035.fdocuments.us/reader035/viewer/2022062519/5697bfe91a28abf838cb6cfd/html5/thumbnails/8.jpg)
Abstract
• First impression to the reviewers
• Concisely describe every part of your paper
• Write it last, following the logic of the proposal
![Page 9: Proposal writing. What is your question Why is your study important How are you going to do it Key Points of Your Proposal Who’s the boss.](https://reader035.fdocuments.us/reader035/viewer/2022062519/5697bfe91a28abf838cb6cfd/html5/thumbnails/9.jpg)
.
Early onset breast cancer has been the unique and major health problem in Taiwan with annual increase-----
The etiology and mechanisms of breast cancer in young women is unknown. It has been suggested that carcinogenesis in utero may account for the early-onset of this cancer-------------
To test this hypothesis, we have used fed pregnant mice with fatty diet and found increase in incidence of mammary tumors in the new born mice.----------------------
In this proposal, we will examine the alteration of mammary gland differentiation pathway in the mammary tumors derived from the new born mice using microarray, SAGE (serial analysis of gene expression) as well real-time PCR techniques -------------
This study of alterations in mammary gland differentiation in the mammary tumor of new born mice will allow us to identify the genetic and molecular mechanisms involved in the early-onset breast cancer.
This information could be useful for diagnosis/treatment of early-onset breast cancer in Taiwan---------------------------
Importance
Outstanding problems
Hypothesis
Experimental approaches
Summary
Answers
Significance
Background and preliminary data
![Page 10: Proposal writing. What is your question Why is your study important How are you going to do it Key Points of Your Proposal Who’s the boss.](https://reader035.fdocuments.us/reader035/viewer/2022062519/5697bfe91a28abf838cb6cfd/html5/thumbnails/10.jpg)
Hypothesis and Specific Aims
Hypothesis and Specific AimsObjective, rationale, and hypothesis
Specific aim 1
Specific aim 2
Specific aim 3
Hypothesis and Specific AimsObjective, rationale, and hypothesis
Specific aim 1
Specific aim 2
Specific aim 3
List the rationales and objectives of your proposal, and hypotheses to be tested. (Optional: use a overview figure to explain your hypothesis and aims)
Describe concisely and realistically what the specific research is intended to answer the hypothesis.
![Page 11: Proposal writing. What is your question Why is your study important How are you going to do it Key Points of Your Proposal Who’s the boss.](https://reader035.fdocuments.us/reader035/viewer/2022062519/5697bfe91a28abf838cb6cfd/html5/thumbnails/11.jpg)
Hypothesis and Specific Aims
• Begin with a statement of long term overall goal: overview, significance, central hypothesis
• List the specific aims one by one concisely• Not too many aims; 2-4 best• Be specific, no general and ambiguous statements• Be realistic, no unachievable aims; best if supported
by preliminary results• Aims should be logically linked and arranged
accordingly; testing your hypothesis• Link aims to Experimental Design
![Page 12: Proposal writing. What is your question Why is your study important How are you going to do it Key Points of Your Proposal Who’s the boss.](https://reader035.fdocuments.us/reader035/viewer/2022062519/5697bfe91a28abf838cb6cfd/html5/thumbnails/12.jpg)
Hypothesis
• State your hypothesis clearly in the abstract and specific aims.
• Hypothesis should be based on experimental data; Hypothesis non fingo!
• Use relevant literatures and preliminary data to help build the case to support the hypothesis --- no reinventing the wheel
• Seeing is believing: Use schematic figures or diagrams to help reviewers understand your thinking.
![Page 13: Proposal writing. What is your question Why is your study important How are you going to do it Key Points of Your Proposal Who’s the boss.](https://reader035.fdocuments.us/reader035/viewer/2022062519/5697bfe91a28abf838cb6cfd/html5/thumbnails/13.jpg)
Examples for overview figure for hypothesis
![Page 14: Proposal writing. What is your question Why is your study important How are you going to do it Key Points of Your Proposal Who’s the boss.](https://reader035.fdocuments.us/reader035/viewer/2022062519/5697bfe91a28abf838cb6cfd/html5/thumbnails/14.jpg)
Pictures say more than a thousand words!
A good figure can improve proposal clarity. Drawing process clarifies your thinking. If you don’t understand the process, you can’t draw it. Make cartoons of:•A scientific equipment that you are using and an explanation of how it works to solve your problem•a novel physical, chemical or mathematical equations •a flow chart showing the steps in a process and the possible steps, feedback loops, causes and consequences.
![Page 15: Proposal writing. What is your question Why is your study important How are you going to do it Key Points of Your Proposal Who’s the boss.](https://reader035.fdocuments.us/reader035/viewer/2022062519/5697bfe91a28abf838cb6cfd/html5/thumbnails/15.jpg)
Specific aim 1
Specific aim 2
Specific aim n
Subaim 1a
Subaim 1b
Subaim 1n
Paragraph
logic
logic
Specific aims
Sections
Proposal
tests
![Page 16: Proposal writing. What is your question Why is your study important How are you going to do it Key Points of Your Proposal Who’s the boss.](https://reader035.fdocuments.us/reader035/viewer/2022062519/5697bfe91a28abf838cb6cfd/html5/thumbnails/16.jpg)
When you divide a section into subsections, all the pieces should be of the same pie
![Page 17: Proposal writing. What is your question Why is your study important How are you going to do it Key Points of Your Proposal Who’s the boss.](https://reader035.fdocuments.us/reader035/viewer/2022062519/5697bfe91a28abf838cb6cfd/html5/thumbnails/17.jpg)
Specific Aims
Specific Aim #1. Identification of downstream genes involved in ----. This purpose of this aim will test the hypothesis that-------. This study will be able to allow us to identify ----- Subaim 1a: Yeast two-hybrid technique will be used to ----- Subaim 1b: Deletion analysis will be used to -----
Title
Hypothesis
Approaches
Importance
![Page 18: Proposal writing. What is your question Why is your study important How are you going to do it Key Points of Your Proposal Who’s the boss.](https://reader035.fdocuments.us/reader035/viewer/2022062519/5697bfe91a28abf838cb6cfd/html5/thumbnails/18.jpg)
Specific Aim 1. To determine the role of nucleosomes in the regulation of Igk locus rearrangement. Our preliminary results showed that the V(D)J recombinase could not recognize RSS targets if they were arranged into a nucleosome structure. We propose experiments to extend these observations by 1) determining what fraction of the Jk gene segments are in the nucleosomal structure in cells undergoing rearrangement as compared with non-lymphoid cells, 2) determining if nucleosomes are phased across the Jk locus, 3) determining whether nucleosome remodeling complex can alter the accessibility of the Jk cluster in native or reconstituted chromatin. This analysis will give us insight of the mechanism of Igk gene rearrangement.
Rationale and hypothesis
Significance
Approaches
Title
![Page 19: Proposal writing. What is your question Why is your study important How are you going to do it Key Points of Your Proposal Who’s the boss.](https://reader035.fdocuments.us/reader035/viewer/2022062519/5697bfe91a28abf838cb6cfd/html5/thumbnails/19.jpg)
Background and Significance Briefly sketch the background of the present proposal, critically evaluate existing knowledge, and specifically identify the gaps which the project is intended to fill.
State concisely the importance of the research, such as health relevance, scientific contribution, uniqueness and originality.
![Page 20: Proposal writing. What is your question Why is your study important How are you going to do it Key Points of Your Proposal Who’s the boss.](https://reader035.fdocuments.us/reader035/viewer/2022062519/5697bfe91a28abf838cb6cfd/html5/thumbnails/20.jpg)
Background
• Information needed for people to understand your proposal.
• Important and interesting problems still standing.
• Hypothesis ( if need detailed explanation)
![Page 21: Proposal writing. What is your question Why is your study important How are you going to do it Key Points of Your Proposal Who’s the boss.](https://reader035.fdocuments.us/reader035/viewer/2022062519/5697bfe91a28abf838cb6cfd/html5/thumbnails/21.jpg)
Funnel approachChecklist approach
How to organize background information
![Page 22: Proposal writing. What is your question Why is your study important How are you going to do it Key Points of Your Proposal Who’s the boss.](https://reader035.fdocuments.us/reader035/viewer/2022062519/5697bfe91a28abf838cb6cfd/html5/thumbnails/22.jpg)
Significance
• State concisely and clearly the importance of your study.
• Emphasize the novelty or innovation.– So what?– I’m the best person to do it
• Convey the significance of your research to– 1) scientific contribution – 2) improving public health – 3) make big money
![Page 23: Proposal writing. What is your question Why is your study important How are you going to do it Key Points of Your Proposal Who’s the boss.](https://reader035.fdocuments.us/reader035/viewer/2022062519/5697bfe91a28abf838cb6cfd/html5/thumbnails/23.jpg)
Preliminary Results
• Show only the relevant experiments supporting your hypothesis
• The results should not be ambiguous• Figure and table legends should be clearly
written; figure numbers should correspond to the text; be sure to label the figures
• Do not show published results in this section
![Page 24: Proposal writing. What is your question Why is your study important How are you going to do it Key Points of Your Proposal Who’s the boss.](https://reader035.fdocuments.us/reader035/viewer/2022062519/5697bfe91a28abf838cb6cfd/html5/thumbnails/24.jpg)
Research Design and Methods
List as specific aims
Aim1 experiment1Aim2 experiment2
Use if you separate aims by different approaches.
List as specific aims
Aim1 experiment1Aim2 experiment2
Use if you separate aims by different approaches.
List as general methods
Experiment1Experiment2
Use when you apply similar approach to multiple aims.
List as general methods
Experiment1Experiment2
Use when you apply similar approach to multiple aims.
![Page 25: Proposal writing. What is your question Why is your study important How are you going to do it Key Points of Your Proposal Who’s the boss.](https://reader035.fdocuments.us/reader035/viewer/2022062519/5697bfe91a28abf838cb6cfd/html5/thumbnails/25.jpg)
Research Design(1)
• Do NOT write as protocol or operation manual• Design the experiments to solve the problems
posed in the specific aims in logical order• Be realistic, do not plan too many experiments
or out of your expertise; manageable; focus! • Be logical; step-by-step leading to your goal• Provide information on statistical analysis
whenever applicable
![Page 26: Proposal writing. What is your question Why is your study important How are you going to do it Key Points of Your Proposal Who’s the boss.](https://reader035.fdocuments.us/reader035/viewer/2022062519/5697bfe91a28abf838cb6cfd/html5/thumbnails/26.jpg)
Research Design(2)
• Updated technologies. • Competence in using techniques proposed• Evaluate the design critically; alternative
approaches, pros and cons• Control! Control! Control!• A schematic diagram will help the reviewers
to understand complicate experimental design
![Page 27: Proposal writing. What is your question Why is your study important How are you going to do it Key Points of Your Proposal Who’s the boss.](https://reader035.fdocuments.us/reader035/viewer/2022062519/5697bfe91a28abf838cb6cfd/html5/thumbnails/27.jpg)
![Page 28: Proposal writing. What is your question Why is your study important How are you going to do it Key Points of Your Proposal Who’s the boss.](https://reader035.fdocuments.us/reader035/viewer/2022062519/5697bfe91a28abf838cb6cfd/html5/thumbnails/28.jpg)
Anticipated Results
• Why good results will be expected • Reasonable time table for achieving results• Alternative Interpretations critically evaluated• How the results will support your hypothesis• Potential difficulties and limitations of the experiment,
and alternative approaches to achieve the aims.• How your results would be important for a group
project
![Page 29: Proposal writing. What is your question Why is your study important How are you going to do it Key Points of Your Proposal Who’s the boss.](https://reader035.fdocuments.us/reader035/viewer/2022062519/5697bfe91a28abf838cb6cfd/html5/thumbnails/29.jpg)
Reference (2 page maximum)
Please choose “Author-date” style in your Endnote:In text: (Almeida et al. 2006)Almeida, C. G., R. H. Takahashi and G. K. Gouras (2006). "-
amyloid accumulation impairs multivesicular body sorting by inhibiting the ubiquitin-proteasome system." J. Neurosci. 26(16): 4277–4288.
• Should correspond to the text• Updated; don’t miss the most relevant ones• Consistent format
![Page 30: Proposal writing. What is your question Why is your study important How are you going to do it Key Points of Your Proposal Who’s the boss.](https://reader035.fdocuments.us/reader035/viewer/2022062519/5697bfe91a28abf838cb6cfd/html5/thumbnails/30.jpg)
What is your question
Hypothesis
Why is your study important
Significance
How are you going to do it
Research design
Key Points of Your Proposal
![Page 31: Proposal writing. What is your question Why is your study important How are you going to do it Key Points of Your Proposal Who’s the boss.](https://reader035.fdocuments.us/reader035/viewer/2022062519/5697bfe91a28abf838cb6cfd/html5/thumbnails/31.jpg)
Benefits of Writing Proposal
• Allow you to review and critically evaluate your current research program
• Convince yourself and others that your research is worth doing
• Keep you focused on your research program• Develop novel ideas during writing proposal
![Page 32: Proposal writing. What is your question Why is your study important How are you going to do it Key Points of Your Proposal Who’s the boss.](https://reader035.fdocuments.us/reader035/viewer/2022062519/5697bfe91a28abf838cb6cfd/html5/thumbnails/32.jpg)
Writing Stages
2. Writing the First Draft2. Writing the First Draft
4. Finishing4. Finishing3. Revising, Revising, Revising3. Revising, Revising, Revising
1. Getting in the Mood1. Getting in the Mood
Save multiple copies of your draft
![Page 33: Proposal writing. What is your question Why is your study important How are you going to do it Key Points of Your Proposal Who’s the boss.](https://reader035.fdocuments.us/reader035/viewer/2022062519/5697bfe91a28abf838cb6cfd/html5/thumbnails/33.jpg)
RO1 grant writing timeline
![Page 34: Proposal writing. What is your question Why is your study important How are you going to do it Key Points of Your Proposal Who’s the boss.](https://reader035.fdocuments.us/reader035/viewer/2022062519/5697bfe91a28abf838cb6cfd/html5/thumbnails/34.jpg)
Proposal deadline
5 月 7 日
![Page 35: Proposal writing. What is your question Why is your study important How are you going to do it Key Points of Your Proposal Who’s the boss.](https://reader035.fdocuments.us/reader035/viewer/2022062519/5697bfe91a28abf838cb6cfd/html5/thumbnails/35.jpg)
How to start?
How to start?How to start: how to get ideas
Goal/Specific problems
How to solve problems
Preliminary data Literature search
RationaleRationale
Hypothesis
Get ideas Get more ideas
![Page 36: Proposal writing. What is your question Why is your study important How are you going to do it Key Points of Your Proposal Who’s the boss.](https://reader035.fdocuments.us/reader035/viewer/2022062519/5697bfe91a28abf838cb6cfd/html5/thumbnails/36.jpg)
Writing a Proposal
• Ideas, Ideas, Ideas! Make a folder and enter thoughts anytime. Also put in papers or any related info.
• Based on facts ; no speculations• Checking in literatures for similar ideas• Evolution of ideas Hypothesis• Obtain preliminary results to support your hypothesis
Initial Planning: start early!
![Page 37: Proposal writing. What is your question Why is your study important How are you going to do it Key Points of Your Proposal Who’s the boss.](https://reader035.fdocuments.us/reader035/viewer/2022062519/5697bfe91a28abf838cb6cfd/html5/thumbnails/37.jpg)
Order of Writing a Proposal
• Title(8)• Abstract(7)• Background and Rationale(2)• Overall Goal and Specific Aims(1)• Significance(3)• Preliminary Results (relevant ones)(4)• Experimental Design(5)• Expected Results(6)• Budget(9)• Check list
![Page 38: Proposal writing. What is your question Why is your study important How are you going to do it Key Points of Your Proposal Who’s the boss.](https://reader035.fdocuments.us/reader035/viewer/2022062519/5697bfe91a28abf838cb6cfd/html5/thumbnails/38.jpg)
When you write
Keep the big picture in mind!!!
Remember who’s the boss !!!
![Page 39: Proposal writing. What is your question Why is your study important How are you going to do it Key Points of Your Proposal Who’s the boss.](https://reader035.fdocuments.us/reader035/viewer/2022062519/5697bfe91a28abf838cb6cfd/html5/thumbnails/39.jpg)
Keep in mind when you write
Overall Goal and Hypothesis: do I know what I’m doing?
why your proposal is important/interesting that deserves to be supported: Extensive literature search. Convince yourself the significance and contribution of your proposal
Specific Aims and experiment design:
How to solve problems I posed .
Do my experiments meet the accept standard?
How will I record the work as it proceeds?
![Page 40: Proposal writing. What is your question Why is your study important How are you going to do it Key Points of Your Proposal Who’s the boss.](https://reader035.fdocuments.us/reader035/viewer/2022062519/5697bfe91a28abf838cb6cfd/html5/thumbnails/40.jpg)
After first draft….
Have others read and critiqueCan they understand the rationale described, its significance, and the procedure proposed? Do they share your view of the study, if not, why?Revise proposal
Double check before turning in: SpellingFormatCitation requirement
![Page 41: Proposal writing. What is your question Why is your study important How are you going to do it Key Points of Your Proposal Who’s the boss.](https://reader035.fdocuments.us/reader035/viewer/2022062519/5697bfe91a28abf838cb6cfd/html5/thumbnails/41.jpg)
What is your question
Why is your study important
How are you going to do it
Key Points of Your Proposal
![Page 42: Proposal writing. What is your question Why is your study important How are you going to do it Key Points of Your Proposal Who’s the boss.](https://reader035.fdocuments.us/reader035/viewer/2022062519/5697bfe91a28abf838cb6cfd/html5/thumbnails/42.jpg)
Reasons Why Proposal is Rejected
• Importance and rationale not clearly stated• The problem is outmoded or trivial• No specific aims! Aims too diffuse or general• Similar experiments have already been done• No evidence for competence to carry out the proposal• Not realistic; overly ambitious plan
![Page 43: Proposal writing. What is your question Why is your study important How are you going to do it Key Points of Your Proposal Who’s the boss.](https://reader035.fdocuments.us/reader035/viewer/2022062519/5697bfe91a28abf838cb6cfd/html5/thumbnails/43.jpg)
Reasons Why Proposal is Rejected
• No experimental design! Or no correlation with the specific aims; poor design
• Too many factors or variables that will give ambiguous interpretations
• No hypothesis; fishing expedition; technique looking for problem
• No Controls• Proposed study will not give useful information or
new contributions• Proposal depends on a key experiment with uncertain
outcome
![Page 44: Proposal writing. What is your question Why is your study important How are you going to do it Key Points of Your Proposal Who’s the boss.](https://reader035.fdocuments.us/reader035/viewer/2022062519/5697bfe91a28abf838cb6cfd/html5/thumbnails/44.jpg)