P r o m e g a

STR Analysis

DNA Isolation

Automation

Successful DNA typing depends on the quality and nature of the sample and purified DNA. The ideal DNA purification protocol separates DNA from the solid support, other biological material and potential PCR inhibitors and is amenable to automation for increased throughput.

Specialized allele-calling software eliminates the need for visual comparisons to assign alleles, making the DNA-typing process faster, easier and more accu-rate. However, analysis of STR data is more than allele calling. Expert systems software increases the efficiency or effectiveness of genotyping by automatically interpreting and reviewing STR data. Expert systems also can objectively evalu-ate DNA mixtures in seconds, flag potential contamination, sum marize data and generate reports.

Genetic Identity Promega Corporation is a global leader in the development of products for genetic iden-tity, including products for forensics, databasing and paternity testing. Many of these products, such as the DNA IQ™ System, can be automated to increase sample through-put. Click on the links below to learn more about sample preparation and quantitation, normalization and PCR setup.

DNA purification methods cannot differentiate between human DNA and contaminating bacterial and fungal DNA. Therefore, if the sample is not pristine and you want to de-termine the human genomic DNA concentration, you will need a human-specific DNA quantitation system. The most common systems are based on real-time PCR.

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DNA Quantitation

Isolation and Growth

Differentiation and Maintenance

Stem cellsThe landscape changes rapidly in the evolv-ing field of stem cell research. We will keep you connected to the stem cell community and help you navigate this evolution with confidence with our robust range of proven technology

Promega offers many monoclonal and polyclonal antibodies for cell regulation, signal transduction and neurobiology. In addition a number of key growth fac-tors for growth and differentiation.

Assays for multiplexing cell viability and cytotoxic-ity, for apoptosis and ADME/TOX studies. For genetic analysis, reliable, robust reagents and systems whatever your amplification needs—from routine PCR to real-time quantitative systems.

Cell authentication is critical component of your data integrity. With leading products in genetic analysis, including the StemElite ID system to detect contamina-tion of mouse feeder cells, you can have confidence in your results.

Functional Profiling

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Drug DiscoveryIn a recent report, researchers at the NCGC describe use of P450-Glo™ Assays to monitor activity of small molecule library compounds stored at room-tempera-ture in DMSO or DMSO:water. They assayed CYP1A2 & 2D6 isoforms in a quantitative HTS format. The homo-geneous P450-Glo assay was faster and more efficient t han the traditional LC-MS method, and gave similar results.

Homogeneous bioluminescent assay. Measures activity of deubiquitinating (DUB), deSUMOylating (SENP) and deneddylating (NEDP) proteases.

Live-Cell Biosensor Technology

for GPCR Studies The new GloSensor™ cAMP Assay is a ‘zero step’

assay for studies on cAMP kinetics.

Ideal for GPCRs that signal through cAMP

Nonlytic, live-cell assay

Gives more biologically relevant data directly from living cells

DUB-Glo™ Protease: An Easy Assay for DUB, SENP or NEDP Activity

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Changing the World on a LARGER SCALE