Project on Micropropagation

8/19/2019 Project on Micropropagation

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PLANT TISSUE CULTURE :

MICROPROPAGATION

TECHNIQUES

Department of biotechnology

N.C.College of Engineering,Israna(Panipat)

Work carried ot nder

Dr. !bash "a#la

!enior !cientific $fficer

Pro#ect %ide,&icropropagation

Pro#ect 't

Centre for Plant iotech.,CC!'*,isar

y

'shish "atyal


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$tline$tline

IntroductionIntroduction

NeedNeed

Stages of MicropropagationStages of MicropropagationExperimental WorkExperimental Work

Applications of Plant Tissue cultureApplications of Plant Tissue culture

Results & DiscussionsResults & Discussions


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IntrodctionIntrodction

What is Plant TissueWhat is Plant Tissue

CultureCulture

It is the technique of growing plant cells,tissues, organs, seedsor other plant parts in a sterile environmenton a nutrient medium


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Plant Tissue Culture = Plant Cloning


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W! We Need Tissue "ulture#

Quick multiplication of plants that are rare, elite orface extinction

Progen! is disease free " true to t!pe

#igh multiplication rates

$xport of high qualit! planting material


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% Totipotent Cell

&organ in '()' gave the term *Totipotent

Cell+ impl!ing that a cell is capale ofdeveloping into a whole organism$

Plant Cells are Totipotent

In '()- #aerlandt .

/ave Practical shape to

Totipotenc! Concept

%ttempted to culture cells


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0tages ) to 1

Stage 1: $xplants in initiation medium

Stage 2: 2ewl! formed ud, shootlet or callusare cultured on multiplication media

Stage 3: 0hootlets that are formed aretransferred to rooting media

Stage 4: 3ooted plantlets are hardened

0tage ). /row the parent plants in sterileconditions or glasshouse

Stages of MicropropagationStages of Micropropagation


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Tissue Culture 0tages


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/$2$3%4 P35C$673$

$xplants Collected

Inoculated in *Initial ud proliferation medium+

Transferred to &ultiplication medium

0u8cultured, rooted, hardened and planted


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&icropropagation

4arge scale multiplication of high value plantmaterial

2ormall! use meristem as explant

meristem is the growing point of a plant

consists of cells which are undifferentiated

%ll regenerated material are clones


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&icropropagation

%llows for continuous production

Permits seasonal production

%llows for long term preservation of plants

0mall space requirements

9ecause the meristem is starting material,products are virus and disease free


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Experimental WorkExperimental Work

TI007$ C74T73$ I2 07/%3C%2$

STEPS IN%'%ED(

Wasing

Explant Preparation

Media Preparation

Sterili)ation

Inoculation*ardening


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Washing


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$xplant Preparation


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&edia Preparation

C i i f &0 di


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Composition of &0 media:&urashige and 0koog, '(;-


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Macronutrients mg/1

(NH4)NO3 1,650K NO

31,900

CaCl2.2H

2O 440

MgSO4.6H

2O 370

KH2PO4 170

Iron mg/l

Na2!"# 33.6

$%SO4.7H2O 27.8


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Vitamins mg/l

n&'o&n&' a'& 0.05*+&o-&n%.HCl 0.05

&a/&n%.HCl 0.01

Cytokinin mg/l

K&n%&n 0.1


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mg/l l+'&n% 0.2

myo-&no&ol 100

## 10Su'o% 30,000

#ga (0.8 )

*H 5.7

Other components


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0terili>ation


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0terili>ed &edia 0torage


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Inoculation


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Primar! 0torage


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0econdar! 0torage


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#ardening


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#ardening 5f Tissue Culture Plants


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%pplications of Plant Tissue culture%pplications of Plant Tissue culture

#as een referred to as#as een referred to as iotechnolog! of developingiotechnolog! of developing

worldworld

4ow capital4ow capital costs relative to other technologiescosts relative to other technologies

#igh laor input#igh laor input :laor is cheap in most developing:laor is cheap in most developing

countries


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3esults


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5f Course@Challenges and

5pportunitiesare alwa!s

two sides

of the same coin


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Project on Micropropagation

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