Progress Report on Miniaturized Liquid Chromatography for NeSSI Scott Gilbert, CPAC Visiting Scholar...
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Transcript of Progress Report on Miniaturized Liquid Chromatography for NeSSI Scott Gilbert, CPAC Visiting Scholar...
![Page 1: Progress Report on Miniaturized Liquid Chromatography for NeSSI Scott Gilbert, CPAC Visiting Scholar Crystal Vision Microsystems LLC Atofluidic Technologies,](https://reader035.fdocuments.us/reader035/viewer/2022062801/56649e4c5503460f94b41fc7/html5/thumbnails/1.jpg)
Progress Report on Miniaturized Liquid
Chromatography for NeSSI
Scott Gilbert, CPAC Visiting ScholarCrystal Vision Microsystems LLC
Atofluidic Technologies, LLC
![Page 2: Progress Report on Miniaturized Liquid Chromatography for NeSSI Scott Gilbert, CPAC Visiting Scholar Crystal Vision Microsystems LLC Atofluidic Technologies,](https://reader035.fdocuments.us/reader035/viewer/2022062801/56649e4c5503460f94b41fc7/html5/thumbnails/2.jpg)
Lab-on-chip approach
Simplicity of operation Reduction of moving
parts – no (mechanical) pumps
Robustness Inexpensive and facile
maintenance
![Page 3: Progress Report on Miniaturized Liquid Chromatography for NeSSI Scott Gilbert, CPAC Visiting Scholar Crystal Vision Microsystems LLC Atofluidic Technologies,](https://reader035.fdocuments.us/reader035/viewer/2022062801/56649e4c5503460f94b41fc7/html5/thumbnails/3.jpg)
Chip Layout20
mm Column
Inlet lines
Detector electrodes
![Page 4: Progress Report on Miniaturized Liquid Chromatography for NeSSI Scott Gilbert, CPAC Visiting Scholar Crystal Vision Microsystems LLC Atofluidic Technologies,](https://reader035.fdocuments.us/reader035/viewer/2022062801/56649e4c5503460f94b41fc7/html5/thumbnails/4.jpg)
From Old Setup…
![Page 5: Progress Report on Miniaturized Liquid Chromatography for NeSSI Scott Gilbert, CPAC Visiting Scholar Crystal Vision Microsystems LLC Atofluidic Technologies,](https://reader035.fdocuments.us/reader035/viewer/2022062801/56649e4c5503460f94b41fc7/html5/thumbnails/5.jpg)
…To New NeSSI Mountable Design
![Page 6: Progress Report on Miniaturized Liquid Chromatography for NeSSI Scott Gilbert, CPAC Visiting Scholar Crystal Vision Microsystems LLC Atofluidic Technologies,](https://reader035.fdocuments.us/reader035/viewer/2022062801/56649e4c5503460f94b41fc7/html5/thumbnails/6.jpg)
Split flow approach to sampling
Liters per minute
microliters per minute
nanoliters per minute
![Page 7: Progress Report on Miniaturized Liquid Chromatography for NeSSI Scott Gilbert, CPAC Visiting Scholar Crystal Vision Microsystems LLC Atofluidic Technologies,](https://reader035.fdocuments.us/reader035/viewer/2022062801/56649e4c5503460f94b41fc7/html5/thumbnails/7.jpg)
Fluid Flow Strategy
![Page 8: Progress Report on Miniaturized Liquid Chromatography for NeSSI Scott Gilbert, CPAC Visiting Scholar Crystal Vision Microsystems LLC Atofluidic Technologies,](https://reader035.fdocuments.us/reader035/viewer/2022062801/56649e4c5503460f94b41fc7/html5/thumbnails/8.jpg)
Functional Integration of -fluidic LC Chip for On-line Sample Pretreatment
micromixer
diluent in
column
mobile phase in
sample in
![Page 9: Progress Report on Miniaturized Liquid Chromatography for NeSSI Scott Gilbert, CPAC Visiting Scholar Crystal Vision Microsystems LLC Atofluidic Technologies,](https://reader035.fdocuments.us/reader035/viewer/2022062801/56649e4c5503460f94b41fc7/html5/thumbnails/9.jpg)
Rapid Prototyping Using Quick Photolithographic Method
Inkjet printed photomask Finished product
![Page 10: Progress Report on Miniaturized Liquid Chromatography for NeSSI Scott Gilbert, CPAC Visiting Scholar Crystal Vision Microsystems LLC Atofluidic Technologies,](https://reader035.fdocuments.us/reader035/viewer/2022062801/56649e4c5503460f94b41fc7/html5/thumbnails/10.jpg)
Front Mounted Microfluidic Chip
![Page 11: Progress Report on Miniaturized Liquid Chromatography for NeSSI Scott Gilbert, CPAC Visiting Scholar Crystal Vision Microsystems LLC Atofluidic Technologies,](https://reader035.fdocuments.us/reader035/viewer/2022062801/56649e4c5503460f94b41fc7/html5/thumbnails/11.jpg)
Back-Mounted Valves
![Page 12: Progress Report on Miniaturized Liquid Chromatography for NeSSI Scott Gilbert, CPAC Visiting Scholar Crystal Vision Microsystems LLC Atofluidic Technologies,](https://reader035.fdocuments.us/reader035/viewer/2022062801/56649e4c5503460f94b41fc7/html5/thumbnails/12.jpg)
Quantitation of flow splitting
Secondary Flow vs. Main Flow
0.00
0.50
1.00
1.50
2.00
2.50
3.00
0 50 100 150 200 250 300
Main Flow (ml)min)
Se
co
nd
ary
Flo
w (
ml/m
in)