Program will begin shortly… “Inner Life of the Cell”, Harvard University © 2008 Up Next …...
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Transcript of Program will begin shortly… “Inner Life of the Cell”, Harvard University © 2008 Up Next …...
Program will begin shortly… “Inner Life of the Cell”, Harvard University © 2008
Up Next …
Making Molecular Movies with Up Next …
Making Molecular Movies with
Insert video: InnerLifeTrimmed.m4vInsert video: InnerLifeTrimmed.m4v
Making Molecular Movies with
Making Molecular Movies with
Your Name, Institution
The Team The Team
Peter KnerPeter KnerMicroscopistMicroscopist
Jessica WinterJessica Winter Chemical EngineerChemical Engineer
Ge YangGe Yang BioengineerBioengineer
Beth BrainerdBeth BrainerdBiologistBiologist
University of Georgia
qstorm.orgVisit us online!
qstorm.orgVisit us online!
Time is ticking. Can these folks make a major breakthrough in
biological imaging?
48 hours in 40 seconds
Credit: www.broad.com; The Ark Hotel
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10 microns
Credit: Michael W. Davidson and Florida State University. Courtesy of the Hamamatsu Learning Center
10 microns
Credit: Michael W. Davidson and Florida State University. Courtesy of the Hamamatsu Learning Center
Insert video: CellDivision-Short.m4vInsert video: CellDivision-Short.m4v
10 microns
Credit: Michael W. Davidson and Florida State University. Courtesy of the Hamamatsu Learning Center
Insert video: CellDivision-Short.m4vInsert video: CellDivision-Short.m4v
10 microns
Credit: Michael W. Davidson and Florida State University. Courtesy of the Hamamatsu Learning Center
Insert video: CellDivision-Short.m4vInsert video: CellDivision-Short.m4v
10 microns
Credit: Michael W. Davidson and Florida State University. Courtesy of the Hamamatsu Learning Center
Insert video: CellDivision-Short.m4vInsert video: CellDivision-Short.m4v
The Team The Team
Peter KnerPeter KnerMicroscopistMicroscopist
Jessica WinterJessica Winter Chemical EngineerChemical Engineer
Ge YangGe Yang BioengineerBioengineer
Beth BrainerdBeth BrainerdBiologistBiologist
University of Georgia
Beth BrainerdBiologist
(Muscle Cells)
Zebrafish Muscle Cells
Credit: Mezzotint
Insert video: loop-and-slow-motion-female-sp_prores.movInsert video: loop-and-slow-motion-female-sp_prores.mov
Ge YangBioengineer (Nerve Cells)
Credit: “Inside the Brain”, NIH National Institute on Aging
Nerve Cells
Insert video: InnerLifeTrimmed.m4vInsert video: InnerLifeTrimmed.m4v
“Inner Life of the Cell”, Harvard University © 2008
“Inner Life of the Cell”, Harvard University © 2008
≈ 100 nanometers
“Inner Life of the Cell”, Harvard University © 2008
≈ 100 nanometers
Motor Protein
Much smaller than a wavelength of visible light
30 nm
Point light source
30 nm
Optical Limit: 200 nanometers
200 nm
Overlapping Blurs
200 nm
The Team The Team
Peter KnerPeter KnerMicroscopistMicroscopist
Jessica WinterJessica Winter Chemical EngineerChemical Engineer
Ge YangGe Yang BioengineerBioengineer
Beth BrainerdBeth BrainerdBiologistBiologist
University of Georgia
Peter KnerMicroscopist
STORM
Credit: MovingImages
Insert video: massive-electrical-storm-light_prores.m4vInsert sound: Perfect Thunder Storm-Sound.mp3
Insert video: massive-electrical-storm-light_prores.m4vInsert sound: Perfect Thunder Storm-Sound.mp3
STORMIMAGING
STORM
STochastic
Optical
Reconstruction
Microscopy
STORM
Xiaowei Zhuang 2006
Credit: FredField.com
Standard optical microscope image
Credit: Bates et al. Science 317, 1749-1753 (2007)
3 μm
Loss of resolution when zoomed into the nanoscale
Credit: Bates et al. Science 317, 1749-1753 (2007)
Credit: Bates et al. Science 317, 1749-1753 (2007)
Standard Microscope Image Diffraction-Limited
STORM ImageSuper Resolution
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Localization
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Insert video: Particle Detection.m4vInsert video: Particle Detection.m4v
Greetings from
Insert video: Reconstructed Image.m4vInsert video: Reconstructed Image.m4v
Credit: Ricardo Henriques, Institut Pasteur
STORM Imaging at Work
Stochastic (Random)Blinking
Reconstruction
Insert video: Particle Detection.m4v (on left side) and Reconstructed Image.m4v (on right side)
Insert video: Particle Detection.m4v (on left side) and Reconstructed Image.m4v (on right side)
Insert video: Particle Detection.m4v (on left side) and Reconstructed Image.m4v (on right side)
Insert video: Particle Detection.m4v (on left side) and Reconstructed Image.m4v (on right side)
Credit: Ricardo Henriques, Institut Pasteur
STORM Imaging at Work
Particle Detectionand Localization
Reconstruction
STORM Imaging of Cell Structures
Credit: Rust et al. Nature Methods 3, 793-795 (2006) and Bates et al. Science 317, 1749-1753 (2007)
Insert video: Zhuang_storm_short_slow.aviInsert video: Zhuang_storm_short_slow.avi
Credit: Bates et al. Science 317, 1749-1753 (2007)
STORM – Depends on bright light sources
Credit: Bates et al. Science 317, 1749-1753 (2007)
Less than 30 nm !
STORM – Depends on bright light sources
Credit: Bates et al. Science 317, 1749-1753 (2007)
STORM – How to Get Even Greater Resolution?
Less than 30 nm !
We need more photons!
The Team The Team
Peter KnerPeter KnerMicroscopistMicroscopist
Jessica WinterJessica Winter Chemical EngineerChemical Engineer
Ge YangGe Yang BioengineerBioengineer
Beth BrainerdBeth BrainerdBiologistBiologist
University of Georgia
Jessica WinterChemical Engineer
Quantum Dots in Solution
Credit: Felice Frankel
Nano Crystals of Different Sizes
Jessica WinterChemical Engineer
Credit: Ricardo Henriques
Insert video: Particle Detection.m4vInsert video: Particle Detection.m4v
10 nm
Linker
SuperHeroes
Insert video: vim-NEiA_Fall 2010_MoS_QSTORM_Final.mov
Or download or play from: http://vimeo.com/18462954
Insert video: vim-NEiA_Fall 2010_MoS_QSTORM_Final.mov
Or download or play from: http://vimeo.com/18462954
qstorm.orgVisit us online!
An MOS Strategic Projects Productionby C.L. Alpert and K. Thatewith assistance from J. Antilland the QSTORM Research Collaborative
Production of this project was supported by the National Science Foundation, MCB-1052733. Dissemination of this project was supported by the NISE Network through the National Science Foundation under Award No. 0940143.Any opinions, findings, and conclusions or recommendations are those of the author(s) and do not necessarily reflect the views of the Foundation.
Published under a Creative Commons Attribution-Noncommercial-ShareAlike license: http://creativecommons.org/licenses/by-nc-sa/3.0/us
Join us at www.QSTORM.org
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