Program in NHP Neuroimaging
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Transcript of Program in NHP Neuroimaging
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Program in NHP Program in NHP NeuroimagingNeuroimaging
Calum AvisonCalum Avison
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OverviewOverview RationaleRationale
BiophysicsBiophysics Biology / behaviorBiology / behavior
Facilities / CapabilitiesFacilities / Capabilities ImagingImaging Support EquipmentSupport Equipment StaffStaff
ProjectsProjects DTI Tractography ValidationDTI Tractography Validation Rhesus (3T) - neurodevelopmental consequences of cocaine exposure Rhesus (3T) - neurodevelopmental consequences of cocaine exposure in in
uteroutero Squirrel monkey (9.4T) - neural correlates of tactile illusion / correlation Squirrel monkey (9.4T) - neural correlates of tactile illusion / correlation
with optical imaging and electrophysiologywith optical imaging and electrophysiology
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NHP Rationale - BiologyNHP Rationale - Biology
Structural Homology?Structural Homology? Functional Homology?Functional Homology?
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Project 1 - Prenatal Cocaine Project 1 - Prenatal Cocaine Exposure (PNCE)Exposure (PNCE)
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Prenatal Cocaine Exposure in RhesusPrenatal Cocaine Exposure in Rhesus
Developmental neurobiologyDevelopmental neurobiology PNCE PNCE behavioral/cognitive impairment? behavioral/cognitive impairment? StructureStructure Functional circuitryFunctional circuitry Pre-/post-synaptic defects in neurotransmissionPre-/post-synaptic defects in neurotransmission
MethodologyMethodology PET mapping of receptors, neurotransmitter release PET mapping of receptors, neurotransmitter release High resolution structural MRI + VBMHigh resolution structural MRI + VBM High resolution DTIHigh resolution DTI fMRI responses to pre-/post-synaptic agentsfMRI responses to pre-/post-synaptic agents Co-registrationCo-registration
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Co-registered (ABA) PET/MR images Co-registered (ABA) PET/MR images of Rhesus Monkeyof Rhesus Monkey
T1-weighted MR image acquiredon a human 3T scanner
Summed image from a 3-hracquisition after 4.9 mCi injection of the D2 receptorligand [F-18]fallypride
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HARDI / FORECAST HARDI / FORECAST - NHP (92 directions)- NHP (92 directions)
p_2_17p_2_22p_2_27p_2_31
1.5 mm isotropic2ch FLEX-M coilSENSE, R=1.5
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DTI TractographyDTI Tractography
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HARDI / FORECAST HARDI / FORECAST - NHP (92 directions)- NHP (92 directions)
p_2_17p_2_22p_2_27p_2_31
0.8 mm isotropic2ch FLEX-M coilSENSE
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Implementation on Intera Achieva Implementation on Intera Achieva 3T 3T
Humans - 2 mm isotropic; 32, 92 directionsHumans - 2 mm isotropic; 32, 92 directions Neonatal developmentNeonatal development
Normal and abnormalNormal and abnormal Developmental abnormalitiesDevelopmental abnormalities Correlation with ss-fMRI (structure begets function)Correlation with ss-fMRI (structure begets function)
NHPs - 0.8 mm isotropic; 92 directionsNHPs - 0.8 mm isotropic; 92 directions Validation of HAR diffusion methodsValidation of HAR diffusion methods Structural homology (y/n?) with humansStructural homology (y/n?) with humans
y y Model of human brain Model of human brain n n Brain evolution Brain evolution
Effects of prenatal cocaine exposureEffects of prenatal cocaine exposure
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HARDI / FORECAST HARDI / FORECAST - Human (92 directions)- Human (92 directions)
h_03
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HARDI / FORECAST HARDI / FORECAST Human vs NHP (92 directions)Human vs NHP (92 directions)
h_03 p_2_17
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HARDI / FORECAST HARDI / FORECAST Human vs NHP (92 directions)Human vs NHP (92 directions)
h_09 p_2_22
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HARDI / FORECAST HARDI / FORECAST Human vs NHP (92 directions) Human vs NHP (92 directions)
h_10 p_2_25
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NHP at 3TNHP at 3T
•2ch FLEX-M coil
•SENSE
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NHP at 3TNHP at 3T
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NHP at 3TNHP at 3T
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NHP at 3TNHP at 3T
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Coregistration
(Overlay)
functional 2D anatomic
Draw Volumes-of-interest
- Look at timecourse information for each VOI
- Statistics: GLM, ROI analysis
Coregistration using gyri information to match 2D and 3D
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Volumes-of-interest
Putamen(Divided into RH and LH)
Caudate head(Divided into RH and LH)
supragenual anterior cingulate cortex (ACC1)
Subgenual anterior cingulate cortex (ACC2)
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BrainVoyager Capability
Graph and Table of beta weight values
General Linear Model
Can apply statistics and obtain timecourse of only significantly activated voxels. (above: TC of significantly activated voxels of all six determined volumes-of-interest. 1.0 mg Cocaine administered at volume 200.)