1

PROGRAM BOOK

INTERNATIONAL SEMINAR AND

WORKSHOP ON ADVANCED CELLULAR

AND MOLECULAR BIOLOGY TECHNIQUES

6 - 7 JULY 2017

INTEGRATED BIOMEDICAL LABORATORY

FACULTY OF MEDICINE

UDAYANA UNIVERSITY

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FOREWORD

Dear colleagues

Welcome to the Faculty of Medicine Udayana University

Welcome to the International Seminar and Workshop on Advanced Cellular and Molecular

Biology Techniques

As we all know, cellular and molecular biology have an important theoretical and practical

contribution in the field of science, particularly health and medicine. Cellular and molecular

techniques had been developed very quickly in the field of agricultural biology, farm biology,

pharmacy, veterinary and medicine. Both of them have a great contribution in explaining the

basis of life on earth and how to use that knowledge for human wellbeing.

This seminar held as continuation of our understanding about important role of biomedical

science. We hope there will be a great discussion about recent advances in biological science

and its applications related with health science and disease. Highly reputable foreign and

native speakers are invited to share their knowledge about the advancement in biomolecular

science and techniques. There are free papers as well as poster presentations from many

researchers in Indonesia that have a great contribution on the ongoing discussion on

molecular and cellular biology topics.

The seminar is commenced with a workshop on theme “Detection of gene

polymorphism” and “Choosing a Proper ELISA Method” as a part to learn methods that are

commonly used in molecular and cellular biology areas.

This book program provides you schedule of the event, abstract and biography of invited

speakers and abstract of free papers and posters.

We would like to express our gratitude to all sponsors. Those sponsors are an invaluable part

of our work. Their support helps us generate the palpable energy of the seminar and

workshop.

We hope you have a fruitful discussion and an enjoyable seminar and workshop!

Ni Nyoman Ayu Dewi

Chair

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TABLE OF CONTENT

Page

Foreword 2

Table of Content………………………………………………………………. 3

Schedule ……………………………………………………………………..

Workshop 4

Seminar 5

Paralel Oral Presentation 6

Poster Presentation 7

Abstract of Invited Speaker

Cellular and Molecular Biology Techniques:

From basic to advanced methods (Sangkot Marzuki) 9

Over-expression of Proteins in E.coli (Wen-Chien Lee 10

Hepatitis B Drug Resistance: Molecular Technique for

Detection (I Dewa Nyoman Wibawa) 11

Novel Understanding of Cellular and Molecular Basis of

Sepsis Pathophysiology: A Proposal of New Treatment Strategy

(Masahiro Nishibori) 13

Next Generation Sequencing Application in Medicine

(Joanne Ngeow) 14

Novel Pathogen Identification by Matrix Assisted

Laser Desorption/Ionization-Time of Flight

(Marut Tangwattanachuleeporn) 15

Stem Cell Therapy as an Alternative in Anti-Aging and

Regenerative Medicine (Indra Bachtiar) 16

Curriculum Vitae of Invited Speaker 17

Abstract of Oral Presentation 25

Abstract of Poster Presentation 51

Committee 67

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SCHEDULE

WORKSHOP

Thursday, 6th July 2017

Time Activity Speaker/PIC

8.00-8.30 Registration Committee

8.30-9.15 Polymorphism and PCR Ayu Dewi/Taufik

9.15–9.30 BREAK

9.30-10.30 Hands on DNA purification Team

10.00-10.30 ELISA Step 2 Team

10.30-11.00

Hands on PCR (Polymorphism

Detection)

Team

10.30-11.00 Hands on DNA concentration

measurement

Taufik

11.00-11.45 ELISA Wande

12.00-12.15 ELISA Step 3

12.15-13.00 LUNCH BREAK

13.15-13.30 ELISA Step 4 Team

14.00-14.15 ELISA Step 5 Team

14.15-15.15 Electrophoresis Team

15.00-15.15 ELISA reading Team

15.15-15.30 BREAK

15.30-16.15 Sequencing analysis Ayu Dewi

16.15-selesai Discussion and closing Team

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SCHEDULE

SEMINAR

Friday, 7th July 2017

Time Venue Person In

Charge

07.30-08.30 Registration Secretariat

Division

08.30-09.00 Pembukaan

- Welcome Dance

- Welcome speak from Chair

- Welcome speak and opening by Rector

Event/Seminar

Division

09.00-10.00 Keynote speaker

Prof. Dr. Sangkot Marzuki (Cellular and

Molecular Biology Techniques : From basic to

advanced methods)

Moderator ( dr.

Mariadi, Sp.PD)

10.00-10.30 Coffee break

10.30-11.00 Dr. Joanne Ngeow Yuen Yie, MBBS,M.Med,

MRCP,FAMS,MPH (Next Generation

Sequencing Application in Medicine)

11.00-11.30 Prof. Dr.dr I Dewa Nyoman Wibawa, SpPD-

KGEH (Molecular biology techniques in

clinical setting)

11.30-12.00 Indra Bachtiar, Ph.D (Stem Cell Technology)

12.00-12.30 Panel Discussion

12.30-14.00 ISHOMA

14.00-14.30 Prof. Wen-Chien Lee, PhD (Over- Expression

of Proteins in E.coli)

Moderator (dr.

Komang Januarta

14.30-15.00 Prof. Masahiro Nishibori, M.D, PhD (Novel

Understanding of Cellular and Molecular basis

of Sepsis Pathophysiology: a proposal of new

treatment strategy

15.00-15.30 Asst. Prof Marut Tangwattanachuleeporn, Dr.

rer.nat (Novel pathogen Identification by matrix

assisted laser decorption/ionization-time of

flight

15.30-16.00 Panel Discussion

16.00-17.00 Parallel Discussion

18.00-finish Closing

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PARALLEL ORAL PRESENTATION

Time and Place Speaker Person in Charge/Moderator

Time :16.00-17.00

Place : Ruang Sidang FK

(Health)

1. AA Mas Putrawati

Triningrat

2. Bagus Komang

Satriayasa

3. Yuliana Heri Suselo

4. Dordia Anindita

Rotinsulu

5. Silvi Dwi Anggraini

Moderator : dr. I Kadek

Swastika, M.Kes

Committee : I Made

Suardana

Time : 16.00-17.00

Place : Ruang Histologi

(Health)

1. Mahadewa Tjokorda BG

2. Made Kurnia Widyastuti

Giri

3. I Nyoman Suarsana

4. Elysanti Dwi Martadiani

5. Desak Made Wihandani

Moderator : Dr. dr I

Nyoman Wande, Sp.PK

Committee : I Gede

Wiranatha

Time : 16.00-17.00

Place : Ruang Bahasa

(Health)

1. Luh Putu Iin Indrayani

Maker

2. I WP Sutirta Yasa

3. I Made Jawi

4. I Kadek Swastika

5. Ni Wayan Tianing

Moderator : dr. I Wayan

Surudarma, M.Si

Committee : I Dewa Ketut

Alit Sumarsana

Time : 16.00-17.00

Place : Ruang kelas 401

Agriculture, Nutrion, And

Environment

1. Sheila Simanjuntak

2. I Ketut Berata

3. Ida Bagus Made

Artadana

4. Lili Anggaini

5. I Gusti Ngurah Gede

Putra

6. Sang Gede Purnama

7. Vabera Maslami

Moderator : dr. I Gusti

Kamasan Nyoman Arijana,

M.Si, Med

Committee : I Made Arjana

Note :

- Time for presentation is @ 8 min (presentation + discussion)

- Please give your presentation to the committee when you register at the registration

desk

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POSTER PRESENTATION

No. Abstract Title First Author

1

THE EFFECT OF CACAO BEANS

EXTRACTS ADMINISTRATION ON

SOD AND OX-LDL CONCENTRATION IN OXIDATIVE STRESS

CONDITIONED RAT

Dewi

Wiryanthini IA

2

ANALYSIS OF FOOD USAGE AND NUTRITIONAL STATUS IN RURAL

AREA IN BALI PROVINCE,

INDONESIA

Kadek Tresna

Adhi

3

THE RISK OF CERVICAL

ADENOCARCINOMA IN PATIENTS

INFECTED WITH HUMAN

PAPILLOMAVIRUS TYPE-18

I Gusti Ayu Sri

Mahendra Dewi

4

THE PROFILE OF EPIDERMAL GROWTH FACTOR RECEPTOR

MUTATION DETECTED FROM

CYTOLOGIC SLIDES OF LUNG ADENO CARCINOMA PATIENTS IN

SANGLAH HOSPITAL, BALI

Ni Wayan

Winarti

5

COMBINATION OF HIGHER DOSE

METFORMIN AND LOWER DOSE CISPLATIN INCREASES APOPTOSIS

IN CERVICAL CANCER CELLS LINE

Ratih Dewi Yudhani

6

S GENE MUTATIONS OF HEPATITIS

B VIRUS IN HEPATITIS B CHRONIC PATIENTS AFTER 12 WEEKS OF

TELBIVUDINE TREATMENT

Yenti Purnamasari

7

ETHANOLIC EXTRACT OF THE

POWDER OF RED EARTHWORM (Lumbricus rubellus) OBTAINED FROM

SEVERAL ORGANIC FARMLANDS IN

BALI, INDONESIA: ANALYSIS OF TOTAL PHENOLIC CONTENT AND

ANTIOXIDANT CAPACITY

Ni Wayan Sucindra Dewi

8

Pharmaco-ethological and Hippocampal

CA1 Region Neurohistological Study of Sertraline Effects on Single-prolonged

Stress-induced Rodent Model of PTSD

Agung Nova Mahendra

9

The anticancer activity of (e)-1-(4’-aminophenyl)-3-phenylprop-2-en-1-on

against DMBA-induced mammary cancer

in Sprague Dawley rat through the regulation of microRNA-21 expression.

Ida Ayu Ika Wahyuniari

10

Preparation and in vitro characterization of self-nano emulsifying system of C-

phenylcalix-[4]-resorcinaryl octacinnamate

and C-methylcalix-[4]-resorcinaryl octabenzoate as ultraviolet absorbers

Indrayani Agung Wiwiek

11

Supplementation 2000mg and 1000mg of Linoleic Acid and Alfa Linolenic Acid

Delayed Prediabetic State in Balinese

Young Obese Women: a Randomised Clinical Trial

I W Weta

No Abstract Title First Author

12

CYP2E1 GENOTYPE AND

TRANSAMINASE LEVEL OF TUBERCULOSIS PATIENTS

RECEIVING FIXED DOSE

COMBINATION OF ANTITUBERCULOSIS

I Gusti Ayu

Artini

13

Determinants of Post Traumatic

Stress Disorder (PTSD) Among Traffic Accident Victims in Bali -

Indonesia

Putu Aryani

14

Detection of Citrus Vein Phloem

Degeneration (CVPD) Disease and the Quality of Healthy Fruits in

Nutrient Deficiency of Citrus

I Gede Putu Wirawan

15 Mutation Identification of RpoB Gene from MDR-TB Isolates at

Sanglah Hospital Denpasar, Bali

Dwija, Ida Bagus Nyoman

Putra

16

Hypoglycaemic effects of

zyzygum polyanthum leaves extract on Alloxan-Induced

Through Decrease Of Blood

Glucose And 8-Hydroxy-2 Deoxyguanosine (8-OHdG) In

Hyperglycemic Wistar Rats

Sri Wahjuni

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ABSTRACT OF INVITED

SPEAKERS

9

CELLULAR AND MOLECULAR BIOLOGY TECHNIQUES: FROM BASIC TO

ADVANCED METHODS

Prof. Sangkot Marzuki

Akademi Ilmu Pengetahuan Indonesia (AIPI)

Indonesian Academy of Sciences

ABSTRACT

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OVER-EXPRESSION OF PROTEINS IN E. COLI

Wen-Chien Lee

Department of Chemical Engineering, National Chung Cheng University, Minhsiung 621,

Taiwan

ABSTRACT

The techniques for producing recombinant proteins at a high level in Escherichia coli are

well established. Normally, over-expression is achieved by the integration of the nucleotide

sequence that encodes a protein of interest into a high copy-number plasmid containing the

strong and regulatable promoter and then activated by the addition of an inducer. However, as

a recombinant protein is over-expressed in E. coli, aggregation of polypeptide chains to form

insoluble inclusion bodies (IBs) has been frequently observed. In this presentation, previous

studies on the mechanism of IBs formation and possible sources of cytoplasmic IBs are

reviewed, and the methods to improve the solubility of the over-expressed proteins are

discussed. IBs are conventionally believed to be formed by the irretrievable deposition of

partially folded or improperly folded intermediates and they are thus biologically inactive.

However, biologically active IBs have been observed for many enzymes including

galactosidases, reductases, oxidases, kinases, phosphorylases, and aldolases when over-

expressed in E. coli. The IBs containing proteins with higher conformational quality and

enzymatic activity are thus of great interest. The strategies that can create favorable cellular

conditions for producing active IBs for using as biocatalysts will be thoroughly discussed.

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HEPATITIS B DRUG RESISTANCE:

MOLECULAR TECHNIQUE FOR DETECTION I Dewa Nyoman Wibawa

Gastroentero-hepatology Div., Dept.of Internal Medicine,

Udayana Univ., School of Medicine/

Sanglah General Hospital.

ABSTRACT

In the treatment of chronic hepatitis B in the past, substantial advances have been made. Until

recently, approved treatments for chronic hepatitis B include 2 formulations of interferon and

4 nucleos(t)ide analogues (NAs). Sustained viral suppression is rarely achieved after

withdrawal of a 48-week course of NA therapy, necessitating long, and in many cases,

indefinite treatment with increasing risk of development of drug resistance. Antiviral

resistance and poor adherence are the most important factors in treatment failure of hepatitis

B.

The occurrence of antiviral drug resistance is depends on, what we call as the 4 golden rules

of drug resistance, viral mutation frequency, the intrinsic mutability of the antiviral target

site, the selective pressure exerted by that drug, and the magnitude and rate of virus

replication.

The particular principles of antiviral drug resistance are: single and double mutants preexist;

this is because viral replication generates diversity. Resistance may fade, but not disappear;

that is, when you stop therapy, the resistant virus does not disappear, it is just replaced into a

minor compartment or a minor quasi species; then, when that therapy is reinstigated, it

reappears. So it may fade, but it will not disappear. Partial suppression promotes resistance,

no replication equals no resistance. The polymerase is structurally flexible. Patient

compliance is important.

Monitoring the emergence of antiviral resistance becomes increasingly important especially

with the expanding repertoire of nucleoside/nucleotide analogs for anti-HBV therapy. Severe

hepatitis exacerbations associated with resistance mutations further complicate the picture of

HBV infection. HBV replicates via an RNA intermediate. The lack of proofreading ability of

the RT (HBV polymerase) is attributed to the increased mutational rates in HBV. The

emergence of mutants resistant to antiviral therapy is associated with reduced treatment

efficacy, treatment failure or virological breakthrough.

Genotypic resistance refers to the detection of mutations in HBV DNA that correspond to

changes in amino acid sequence in the reverse transcriptase region of the HBV genome that

have been shown to confer resistance using phenotypic assays, while phenotypic resistance

refers to the decreased susceptibility of HBV polymerase to an antiviral agent in vitro.

Genotypic and phenotypic assay is use for determining HBV drug resistance.

Phenotypic assays for the detection of antiviral resistance are based on the demonstration of

decreased susceptibility of a replication mutant clone to the antiviral agent in vitro as

compared with the wild-type strain. Phenotypic assays offer the advantage of directly

measuring drug susceptibility as well as the cumulative effect of multiple mutations. The

concentration of the antiviral agent required to reduce the replication of the HBV clone

studied to 50% replication is referred to as the IC50. An increase in the concentration of the

antiviral agent required as determined by the IC50 is expressed as 'fold change'.

Genotyping and phenotyping of clinical isolates of HBV is routinely done in many

laboratories worldwide and the results can be expected to aid in design of therapeutic

strategies that maximize the benefit from new antiviral drugs. Resistance testing will also

make an increasing contribution to patient management, especially for individuals who have

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already failed treatment and those who have known risk factors associated with more rapid

emergence of drug resistance.

The availability of advances in methods for HBV DNA quantitation and the early

identification of drug resistance in HBV have been particularly useful in the management of

chronic HBV infection. The decision of switching to appropriate alternative therapies

following early identification of drug resistance will improve the outcome of therapeutic

interventions. The need to better understand the clinical outcome and to minimize the

morbidity in patients with chronic HBV will drive the development of newer molecular

methods.

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NOVEL UNDERSTANDING OF CELLULAR AND MOLECULAR BASIS OF

SEPSIS PATHOPHYSIOLOGY: A PROPOSAL OF NEW TREATMENT

STRATEGY

Masahiro Nishibori

Department of Pharmacology

Okayama University Graduate School of Medicine, Dentistry

and Pharmaceutical Sciences

http://www.okayama-u.ac.jp/user/pharmaco/

ABSTRACT

Sepsis is a systemic inflammatory response syndrome (SIRS) associated with infection. The

pathogenesis of sepsis includes the disturbance of blood-vascular homeostasis, which may

cause multiple organ failure, circulatory shock, and disseminated intravascular coagulation,

leading to high mortality. The proinflammatory cytokine response in the acute phase may be

triggered by the constituents of invading pathogens and tissue damage-associated molecular

patterns, accompanied by the activation of vascular endothelial cells, a pivotal step for

inducing the migration of leukocytes into inflammatory sites with pathogen invasion. A

recent study suggested that neutrophil adhesion on vascular endothelial cells may trigger

platelet aggregation and immunothrombus formation in septic acute respiratory distress

syndrome (ARDS). Thus, circulating neutrophils may play important roles in the

pathogenesis of septic conditions in addition to infiltrating neutrophils. However, the

uncontrolled activation of neutrophils has not been examined in detail due to methodological

limitations.

Recently, we found a novel mechanism of activation of circulating neutrophils and vascular

endothelial cells in septic condition that has shed light on our understanding of septic

pathogenesis. We identified a plasma protein that maintains the circulating neutrophils and

vascular endothelial cells quiescent and decreases markedly during sepsis. Based on these

findings, I will propose a novel therapy for sepsis and multiple organ failure in clinic.

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NEXT GENERATION SEQUENCING APPLICATION IN MEDICINE

Joanne Ngeow

Head, Cancer Genetics Service

Division of Medical Oncology

National Cancer Centre Singapore

From the vantage point of 2017, it is difficult to imagine a time when cancer was not widely

accepted as a genetic disease, in the most basic sense of being caused by alterations in the

structure and function of genes. Indeed, it was not until the second half of the 20th century

that the heritable nature of common cancers started to be widely accepted. Over the decades

since then, genealogic and epidemiologic observations set the stage for a burst of scientific

activity in the 1990s, when developments in positional cloning facilitated the discovery of

genes responsible for the most common cancer predisposition syndromes, and for several less

common ones. These discoveries provided invaluable insights in the biology of not only

inherited cancer but also sporadic disease and led the way for cancer genomics research and

gene-directed management and treatment. Currently, over 400 hereditary cancer

susceptibility syndromes have been described, the majority of which are inherited in an

autosomal dominant manner. Although many of these are rare syndromes, they are thought to

account for at least 5–10% of all cancer, amounting to a substantial burden of morbidity and

mortality in the human population. It is increasingly important that clinicians from all fields

stay abreast of the cancer and non-cancer manifestations of these syndromes. We will review

how next-generation sequencing has revolutionized medical practice allowing us to tailor our

treatments and management according to the genomic profile of patients. We will review the

promise as well as the challenges associated with next generation sequencing in clinical

practice.

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NOVEL PATHOGEN IDENTIFICATION BY MATRIX ASSISTED LASER

DESORPTION/IONIZATION-TIME OF FLIGHT

Marut Tangwattanachuleeporn

Medical Technology Unit, Faculty of Allied Health Sciences,

Burapha University, Chon Buri, Thailand.

ABSTRACT

In few years ago, matrix assisted laser desorption/ionization-time of flight (MALDI-TOF) has

emerged as a fantastic tool for microorganism identification. The principle of this tool is the

analysis of mass spectrometry in ribosomal proteins, which are commonly abundant in the

microbial cell. Then, the mass spectra of ribosomal proteins are analyzed by software and

compared with profile databases of microorganisms. Indeed, this technique has become a

standard method in clinical diagnostic microbiology. Moreover, this technique can be applied

for direct detection of pathogen from clinical specimens (e.g. blood, urine and cerebrospinal

fluid), discovery of antibiotic-resistant pathogens without drug susceptibility test, and

bacterial and fungal typing in epidemiology including generation of phyloproteomic trees.

Microbial identification by MALDI-TOF mass spectrometry is rapid, sensitive, robust,

automatable, and economical in terms of both labor and money. Ultimately, this presentation

will focus in comparison with conventional routine methods and share an idea about its

application in the future.

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STEM CELL THERAPY AS AN ALTERNATIVE IN ANTI-AGING AND

REGENERATIVE MEDICINE

Indra Bachtiar

Stemcell and Cancer Institute (SCI), PT Kalbe Farma Tbk

Jl Ahmad Yani no 2, Pulomas, Jakarta 13210, INDONESIA

ABSTRACT

Aging is an inevitable physiological change. It is a multifactorial process characterized by a

progressive loss of physiological integrity that leads to impaired function and increased

vulnerability to death. Molecular inflammation is an important biological component of

aging, and monitoring the molecules that mediate inflammation may be useful to assess the

aging processes. Specifically, monitoring blood levels of pro-inflammatory cytokines such as

tumor necrosis factor alpha (TNF-α) and interleukin-6 (IL-6) may give us the means to better

understand the aging process. Furthermore, hallmarks of senescent cells include cellular

DNA damage, mitochondrial dysfunction leading to increased reactive oxygen species (ROS)

and decreased ATP, the production of proinflammatory cytokines, telomere shortening, the

trigger of stem cell depletion and cell senescence. Currently, anti-aging therapy with stem

cells as a regenerative medical treatment is proposed as the most effective way to delay

senescence. Stem cell transplantation is generally considered as a highly promising candidate

method for regenerative applications because stem cells possess a high proliferative capacity

and have the potential to differentiate into other cell types. Moreover, previous studies

suggest that accumulated metabolic stress and impaired function of adult stem cells in vivo

are critical for the initiation and development of aging and aging-associated degenerative

diseases. However, understanding the process of stemcell is crucial for efficient expansion

protocols to generate a large number of cells based on traditional culture technic that is

necessary for successful treatment. The aim of this presentation is to take a deep insight into

significant aspects of stemcell-based therapies and to highlight practical limitations that

might impair a safe and efficient clinical application.

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CURICCULUM VITAE OF

INVITED SPEAKERS

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Sangkot Marzuki

Professor Sangkot Marzuki is President of the Indonesian Academy of Sciences, and was

Director of the Eijkman Institute for Molecular Biology in Jakarta from 1992-2014. His

scientific interest has been in the biogenesis of energy transducing membranes, and related

human genetic disorders; an interest he developed during his 17 years as medical faculty

member of Monash University in Australia, before moving to Indonesia in 1992 to rebuild

the Eijkman Institute, where he extended this interest to encompass human genome diversity

and infectious diseases. For his scientific contribution, he received a higher doctorate from

Monash University (1998), and awarded doctor honoris causa from Utrecht University

(2006), and doctor of science honoris causa from the University of Queensland (2010).

Professor Marzuki graduated as a medical doctor from the University of Indonesia in 1968,

obtained his M.Sc. from Mahidol University in Bangkok (1971), and Ph.D. from Monash

University (1975). He has held honorary appointments as a Professor of Medicine at Monash

University (Melbourne), Professor of Medical Sciences at the University of Indonesia in

Jakarta, Professor of Biochemistry and Molecular Biology at the University of Queensland,

and Professor of Medicine at Sydney University. He served in the Indonesian National

Research Council (1994-2004) and Higher Education Council (1996-2000; 2003-2008), as

Governing Council Member of Asia-Pacific International Molecular Biology Network (1998-

2008), and chairman of the Asia Pacific Network of Human Geneticists (1995-2005).

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Wen-Chien Lee

Dr. Wen-Chien Lee is professor in Department of Chemical Engineering and Director of

Systems Biology and Tissue Engineering Research Center at National Chung Cheng

University. He received his B.S. and M.S. in chemical engineering from National Taiwan

Institute of Technology and Ph.D. from Purdue University (1989). He has served as a

visiting professor in Cornell University (USA, 2001) and Kasetsart University (Thailand,

2014) and chairman of Department of Chemical Engineering at National Chung Cheng

University. In past three years, he was the President of the Biotechnology and Biochemical

Engineering Society of Taiwan (BEST) and Deputy Secretary General of the Asian

Federation of Biotechnology (AFOB). Now he is serving as the Honorable President of

BEST to continue his service to biotechnology community. He has published 100

international journal papers and book chapters. His current research interests are proteomics,

biomagnetic particles, production of proteins and biomass-based chemicals.

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I Dewa Nyoman Wibawa

First Name : Prof. Dr. dr. I Dewa Nyoman Last Name: Wibawa, Sp.PD-KGEH

(with degrees)

Date of Birth : November 17th 1952 Married to: dr. Ni Ketut Candra Wiratmi, Sp.S

Office/Institution : Gastroentero-Hepatology Division, Department of Internal Medicine.

Medical Faculty Udayana University/Sanglah General Hospital

Office Address : Jl. Diponegoro, Denpasar Bali

Telephone : 0361-244177 Fax: 0361-244177

E-mail Address : dnwib@dps.centrin.net.id

agusbobwibawa@yahoo.com

Home Address : Jl. Sekar Tunjung II/1 Denpasar Bali

Current Position : Chief of Gastroentero-Hepatology Division, Department of Internal

Medicine. Medical Faculty Udayana University/Sanglah General Hospital

Academic Appointment and Activities:

- Medical Doctor, Udayana University (1979)

- Specialty in Internal Medicine, Diponegoro University (1986)

- Consultant in Gastroentero-Hepatology, Indonesia Association of Internal Medicine

(1997)

- PhD, University of Airlangga (2000)

Professional Activities:

- Doctor in Sanglah General Hospital

- Lecturer in Medical Faculty Udayana University

- Head of Study Program of Internal Medicine, Medical Faculty, Udayana Universitiy

Awards and Qualifications:

- Medika Award (1999)

Society Memberships:

- Indonesian Association of Medical Doctor

- Indonesian Association of Internal Medicine.

- Indonesian Association for the Study of the Liver Diseases.

- Indonesian Association of Gastroenterology.

- Indonesian Association of Digestive Endoscopy

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Masahiro Nishibori

Sex : Male

Birthday : March 16, 1956

Nationality : Japanese

Current Position: Professor, Department of Pharmacology, Okayama University Graduate

School of Medicine, Dentistry and Pharmaceutical Sciences

Address : 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, Japan

Communication: Phone/Fax: +81-86-235-7140

E-mail: mbori@md.okayama-u.ac.jp

Education:

1971-1974 Hikone-higashi High School in Shiga Prefecture

1974-1980 Okayama University Medical School

1985 PhD (Okayama University Graduate School of Medicine)

Employment

1980-1988 Research Associate of Okayama University Medical School

1988-1995 Assistant Professor of Okayama University Medical School

1990-1992 Visiting Scientist of Manitoba Institute of Cell Biology, Manitoba, Canada

1995-2001 Associate Professor of Okayama University Medical School

2001- Professor of Okayama University Graduate School of Medicine, Dentistry, and

Pharmaceutical Sciences

Awards

1983 Yuki Memorial Prize of Okayama Medical Society

1990 Uehara Memorial Research Fellowship (The Uehara Memorial Foundation)

2009 21st Century Invention Award (Japan Institute of Invention and Innovation)

2010 Okayama Prefectural Culture Prize (Academic Category) (Okayama Prefecture)

2012 Bio Business Japan Award 2012 (Saito-Award) (Osaka Prefecture)

2012 Sanyo Newspaper Award (Academic field)

Research Fields: Pharmacology, Drug Development

Academic Activities:

Editor-in-Chief of Journal of Okayama Medical Association (2009-2015)

Associate Editor of Journal of Pharmacological Sciences (2006-2008; 2015 - )

Representative of Forum on Drug Research in Okayama (FDRO) (2004 - )

Member of Japanese Pharmacological Society, Japanese Neuroscience Society,

Japanese Immunology Society, and Society for Neuroscience (USA)

Invited Lectures:

2010 Special Invited Lecture in Annual Meeting of Japanese Pharmaceutical Society

2010 The Fourth International HMGB1 Symposium

2012 Japanese Pharmaceutical Society, Drug development symposium

2012 The 22th Brain Blood Vessel Symposium

2011 Special Invited Lecture in Japanese Neurosurgery Meeting

2014 Special Invited Lecture in Japanese Anesthesiology Meeting

2012 The 22th Brain Blood Vessel Symposium

Publications: 150 peer-reviewed papers

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Joanne Ngeow

Head, Cancer Genetics Service

Division of Medical Oncology

National Cancer Centre Singapore

11 Hospital Drive

Singapore 169610

Dr Joanne Ngeow, MBBS, MRCP, MPH is Senior Consultant,

Division of Medical Oncology at the National Cancer Centre

Singapore and Assistant Professor with the Oncology

Academic Clinical Program, Duke-NUS Graduate Medical

School. Dr Ngeow currently heads the Cancer Genetics Service at the National Cancer Centre

Singapore with an academic interest in hereditary cancer syndromes and translational clinical

cancer genetics.

She was awarded consecutive fellowships by the National Medical Research Council and the

Ambrose Monell Foundation to complete formal clinical and bench training in Cancer

Genomic Medicine at the Genomic Medicine Institute, Cleveland Clinic, Ohio. Dr Ngeow’s

research focuses on understanding how gene-environmental interactions predisposes to

cancer initiation and progression.

23

Marut Tangwattanachuleeporn Burapha University, Division of Microbiology, Department of Medical

Technology, Faculty of Allied Healt Sciences, 169 Longhadbangsaen

Road, Saensuk District, Maung, Chonburi, 20131, Thailand

Marut Tangwattanachuleeporn is a microbiologist currently holding an

Assistant professorship for Microbiology at Burapha University, Chon

Buri, Thailand. He got master degree in medical microbiology with the

highest GPA in the class as 3.94 from Chulalongkorn University,

Thailand and earned Ph.D. degree in Biology from Goettingen University, Germany. In 2011

– 2013, he was a representative for Southeast Asia of the Young ISHAM (the International

Society for Human and Animal Mycology, one of the biggest fungal society). His current

research focus on the prevalence of pathogenic fungi such as Aspergillus spec.,

dermatophytes, or Cryptococcus spec. in the environment as potential sources of infection as

well as monitoring of their antimycotic drug resistance. He had 12 international publications

and 7 national publications (in Thai). Now, he has an administrative position in Burapha

University as Assistant president for research affairs.

24

Indra Bachtiar

EDUCATION

1. Postdoctoral at Laboratorium of Biophysical Chemistry, National Heart, Lung, and Blood Institute (NHLBI), National Institute of Health (NIH), Bethesda, Maryland, USA (2002-2005)

2. Ph.D., Department of Pharmaceutical Molecular Biology, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba, Aramaki, Aoba-ku, Sendai 980-8578, Japan (1998-2002)

PROFESSIONAL POSITIONS Head of Stemcell R&D/Production and Commercialization, REGENIC Lab, PT

Bifarma Adiluhung, Kalbe Farma TbK Principal Investigator at Stem Cell and Cancer Institute Indonesia -SCI-

Indonesia Reviewer at Elsevier Publisher, Bentham Science Publisher, Springer and

BMC-Proteome Science PATENT AND AWARDS 1. MONBUSHO Scholarship, Japan, 1998. 2. Intramural Research Training Award, IRTA, National of Heart, lung and Blood

Institute, NIH, Bethesda, USA, 2003 3. International Stemcell Research Society (ISSCR) 2015 4. Combination of Alpha-1-Acid Glycoprotein and Alpha-Fetoprotein as an

Improved Diagnostic Tool for Hepatocellular Carcinoma”, Patent Registered in USA and Australia.

RECENT JOURNALS/TEXTBOOK CHAPTER (2016 only) TEXTBOOK 1. INTECH Open Science Textbook Chapter, Hypoxia in Mesenchymal Stem

Cell, Chapter 5, Thomson Reuters, ISBN 978-953-51-2896-0. 2016 ARTICLES 2016 1. Wahyu W, Laura W, Dwi A, Harry M, Nurul F, Sutiman B. S, M. Aris W and

Indra B; Biomarkers and Genomic Medicine, In Press, Available online 9 March 2016

2. Mahanani, Erlina Sih, Bachtiar, Indra, Ana, Ika Dewi; Key Engineering Materials, Vol. 696, p205-211. 7p, 2016

25

ORAL PRESENTATION

26

THE COMPARISON BETWEEN LECTURING AND CADRE RECRUITMENT

METHOD TO INCREASE KNOWLEDGE, BEHAVIOR AND ATTITUDE IN

CEMPAGA VILLAGER ABOUT CATARACT BLINDNESS

AA Mas Putrawati Triningrat, AAA Sukartini Djelantik, Ni Made Ari Suryathi

Ophthalmology Department, Faculty of Medicine, University of Udayana

ABSTRACT

Introduction and Objective: Cataract is the highest cause of blindness in Bali. Cempaga

village which located in Singaraja Bali island is original village that have been surveyed for

cataract blindness previously and the number is almost 50%. According to the survey, the

most barrier of cataract surgery is less of knowledge about cataract. The purpose of this study

is to compare the two methods, lecturing and cadre recruitment to increase knowledge,

behaviour and attitude. Methods: This is cross sectional study, and using questionnaire

before and after intervention of two methods Result: Lecturing method statistically

significant to increase knowledge in respondent (p<0,001). Attitude and behaviour seems not

significant increased. In this study, cadre recruitment method not significant to increased

knowledge, attitude and behaviour. Conclusion: In this study we found only lecturing

method can increase significantly knowledge about cataract blindness. We suggest for a long

term study and a regular training for cadre in population to give a good services in order to

improve knowledge, behaviour, attitude concerning eye health especially cataract blindness.

Keywords: lecturing method, cadre recruitment, knowledge, attitude, behaviour, cataract

blindness

27

PURPLE SWEET POTATO TUBER ETHANOLIC EXTRACT DECREASES VCAM

EXPRESSION IN HIGH-CHOLESTEROL DIET-FED RABBITS

Bagus Komang Satriyasa

Department of Pharmacology and Therapy,

Faculty of Medicine, Udayana University

ABSTRACT

Coronary heart disease (CHD) is commonly caused by atherosclerosis. CHD is one of the

most common death-causing diseases globally, especially in developed countries.

Atherosclerosis can be caused by increased expression of proinflammatory cytokines such as

IL-1, IL-6, IL-8, monocyte chemoattractant, VCAM and ICAM, and also enzymes such as

COX-2 and matrix metalloproteinases (MMPs). The aim of this research was to evaluate the

effect of anthocyanin-containing purple sweet potato tuber ethanolic extract in inhibiting

atherosclerosis in rabbits. This study was experimental laboratory study, with randomized

control group post-test-only design, using 16 local Balinese rabbits (4-5 months-old) obtained

from Animal Unit Laboratory. This study found that mean VCAM expressions are 63.3750

and 6.1250, for control (first) and treatment (second) group, respectively. Statistical analysis

showed significant difference in VCAM expression (p<0.05). Therefore, anthocyanin-

containing purple sweet potato tuber ethanolic extract can inhibit atherosclerosis via the

inhibition of VCAM expression.

Keywords: Purple sweet potato ethanolic extract, VCAM, rabbits

28

ANTIOXIDANT POTENTIAL AND HYPOLIPIDEMIC EFFECTS OF COMBINED

PURPLE SWEET POTATO TUBER EXTRACT WITH HONEY IN RATS GIVEN

HIGH CHOLESTEROL FEED

I W P Sutirta Yasa1, I Made Jawi

2*

1.Department of Clinical Pathology, Faculty of Medicine, Udayana University

2.Department of Pharmacology Faculty of Medicine, Udayana University

Denpasar Bali Indonesia.

Corresponding: E-mail: made_jawi@yahoo.co.id

ABSTRACT

Flavonoids prevents cholesterol rising in blood and has antioxidant effects. The purpose of

this study is to prove hypolipidemic and antioxidant effect of purple sweet potato tuber

extract with natural honey combination, which contains flavonoids. This is a true

experimental study using randomized pre-post-test control group design. With 28 adult male

wistar rats, were divided into 4 groups. Before treatment, lipid profiles, MDA, and SOD were

examined as a pre-test data. During the 4 weeks of treatment, Group 1 were given high-

cholesterol feed. Group 2 were given high-cholesterol feed and water extract of purple sweet

potato tubers 200 mg / day. Group 3 were given high-cholesterol feed and natural honey 3 cc

/ day. Group 4 were given high-cholesterol feed and water extract combination of purple

sweet potato tuber with natural honey. After the treatment, the blood were examined again as

the pre-test data. The obtained data were tested using t-test and ANOVA. The results showed

a significant increase of total cholesterol, LDL, and MDA in blood (p <0.05), and

significantly decrease HDL in group 1 (p <0.05). In group 2, 3, and 4 there were no

significant difference of these parameters (p> 0.05), but significantly difference with group 1

(p <0.05). The conclusion is the combination of water extract of purple sweet potato tuber

with honey, can maintain lipid profile and MDA in the normal range, at rats with high-

cholesterol feed.

Keywords: Purple sweet potato, honey, lipid profile, SOD, rat.

29

ENDOGENOUS ANTIOXIDANTS AMONG PROPORTIONAL EXERCISE

Made Kurnia Widiastuti Giri

Ganesha Educational University

Email: drnia82@gmail.com

ABSTRACT Exercise has been declared as one of the natural anti-aging therapies. Studies on the anti-

aging effects of exercise did through many expertise,such one of them is through

immunological studies. The purpose of this study was to analyze the differences in the levels

of specific endogenous antioxidant produced by the mitochondria that was Superoxide

Dismutase (SOD). Methods: This study was a randomized control trial on two group, with a

power of 80%, α = 5% used 20 experimental rats were simple randomly divided into two

groups: sedentary and exercise group proportional (moderate exerciseintensity, 10 minutes a

day, 3 times a week). Treatment was given for 6 weeks and at the end of treatment, blood

examination by using Enzyme Linked Immunosorbent Assay (ELISA) on serum of both

groups. Data were analyzed using parametric statistical tests paired sample t test to test the

difference in results between the dependent variable and the initial observation period after

treatment, while the differences between groups is done with the Chi square test. Results :In

comparison with sedentary groups, proportionated exercise resulted increases performance,

reduction in MDA and increases of SOD (performance 0.6± 5.7 vs 5.2 ± 5.9 vs., p < 0.01;

MDA −5.1 ± 5.9 vs. 3.9± 5.5, p < 0.02; SOD 0.7 ± 2.2 vs 5,6± 6,8. =, p = 0.02). Conclusion:

Among rats in proportioned exercise, we found that participation in an 6-week intervention

was feasible and resulted SOD increases in blood. Thus would be recommended for future

research wtih biomolecular technique analyzing exercise proportinally combined with other

antiaging therapies.

Keywords: exercise, anti-aging, SOD

30

MOLECULAR DETECTION OF AVIAN INFLUENZA VIRUS FROM BIRDS SOLD

IN A MULTISPECIES ANIMAL MARKET JAKARTA

Dordia Anindita Rotinsulu1*

, Surachmi Setiyaningsih1, Abdulgani Amri Siregar

1

1Faculty of Veterinary Medicine, Bogor Agricultural University, Bogor, Indonesia

*corresponding author: dordia.anindita@gmail.com

ABSTRACT Avian Influenza (AI) is a zoonotic disease caused by influenza virus A of the family

Orthomyxoviridae. The Avian Influenza virus infects various birds and mammals, including

human. Multispecies animal market, as a meeting place for human, birds, mammals, and

reptiles can potentially transmit AI virus among animal to human. The purpose of this study

was to detect the presence of Avian Influenza virus (Matrix gene and H5 gene) from various

bird species sold in a multispecies animal market in Jakarta using real-time Reverse

Transcriptase-Polymerase Chain Reaction (rRT-PCR). A number of 862 field samples from

28 families and 96 bird species were collected using cross-sectional sampling technique in a

multispecies animal market, Jakarta. These field samples consisted of 649 fecal samples, 114

cloacal swab samples, and 99 oropharyngeal swab samples. The samples were pooled based

on the type of sample and kiosk where the sample was collected. To detect the presence of AI

virus, molecular technique by using real-time RT-PCR was preformed targeting the matrix

gene of AI virus. Samples which were positive for Matrix gene were further assessed using

real-time RT-PCR targeting the H5 gene for AI virus subtyping. As many as 7 of 649 fecal

samples (1.08%) were positive for Matrix Gene AI Virus. However, none cloacal or

oropharyngeal swab were positive for AI. Positive fecal samples were detected in four bird

species, which are three magpie-robins (Copsychus saularis, family Turdidae), two white-

eyes (Zosterops palpebrosus, family Zostropidae), a yellow-vented bulbul (Pycnonotus

goiavier, family Pycnotidae), and a robin (Leiothrix lutea, family Sylviidae). Based on

molecular detection using real-time RT-PCR, none of the samples were positive for H5 gene

of AI virus.

Keywords: Avian Influenza, birds, matrix gene, real-time RT-PCR.

31

K-RAS MUTATION PROFILE IN COLORECTAL CARCINOMA PATIENTS IN

SANGLAH HOSPITAL

Luh Putu Iin Indrayani M, Ni Putu Sriwidyani

Anatomical Pathology Department, Faculty of Medicine Udayana University/Sanglah

General Hospital

ABSTRACT

Introduction: Today is an era of personalized medicine where treatment is tailored

personalized to each patient status. K-ras mutation is one of molecular examination in

colorectal carcinoma and the result will direct treatment decision to patient. Patient with K-

ras mutation should not to be treated with anti-EGFR. This study was a preliminary study to

determined K-ras mutation profile in colorectal carcinoma in Bali. Methods: Formalin-fixed

paraffin embedded tissue of 23 colorectal carcinoma patients in Sanglah Hospital were

collected in Anatomical Pathology Department and sent to Dr. Cipto Mangunkusumo

Hospital for K-ras examination in the year 2016. All of H-E slides were examined and the

spesimen with maximum of well preserved tumor cells were selected. Mutation of K-ras gene

was performed by PCR. Result: From 23 cases, 70% was male and 30% was female, with

mean age was 48.3 year. Fourteen cases (60.9%) had K-ras mutation (9 cases with codon 12

mutation, 4 cases with codon 13 mutation, 1 case with codon 59 mutation and 1 case with

codon 117 mutation). Conclusion: It is found that K-ras mutation rate was 60.9% and the

two most common mutations observed in codon 12 and 13.

Key words: K-ras, mutation, colorectal cancer, Bali

32

THE EFFECT OF CACAO BEANS EXTRACTS ADMINISTRATION ON SOD AND

OX-LDL CONCENTRATION IN OXIDATIVE STRESS CONDITIONED RAT

Dewi Wiryanthini IA1, Dewi Ratnayanti IGA

2, Sutadarma IWG

1

1Biochemistry Department,

2Histology Department Faculty of medicine Udayana University

Email : dew_wiryanthini@yahoo.com

ABSTRACT

A study in adult Wistar white rats on the effect of extract of cacao beans on the SOD and ox-

LDL level in oxidative stress induce by psychosocial stress was carried out. As many as 20

adult Wistar white rats with oxidative stress induced by psychosocial stress in this study were

divided into 4 groups with 5 rats in each group in Pretest-Postest Control Group Design as

Control group or placebo (P0), intervention group by 70 mg extracts of cacao beans (P1), 140

mg (P2), and 280 mg (P3) for 14 days. All groups were examined for blood SOD and ox-

LDL concentration before and after intervention. This study revealed increase significantly

with p=0,000 on SOD concentration in oxidative stress white rat induced by psychosocial

stress after administration of cacao beans extract in group P1 P1 (535,59 vs 554,24), P2

(540,68 vs 632,2) dan P3 (545,76 vs 696,61) with not significantly decrease of ox-LDL

(p=0,957). This study showed that cacao beans extract can inhibit oxidative stress caused by

psychosocial stress with increased SOD concentration although not effective decreased ox-

LDL.

Keywords: Psychosocial stress, oxidative stress, cacao beans extract, SOD, ox-LDL

33

THE DIFFERENCE IN MOTOR IMPROVEMENTS RELATED TO COMBINATION

OF OMEGA-3 POLYUNSATURATED FATTY ACID AND ALPHA-

TOCOPHEROLSUPPLEMENTATIONSDIET OFWEIGHT-DROPPED INDUCED

SPINAL CORD INJURY IN RATS

Mahadewa Tjokorda GB, Wisnu A. Wardana, Wisnu Wardhana

Neurosurgery Department Udayana University-Sanglah Hospital, Bali, Indonesia

Address correspondence to:TjokordaGB Mahadewa, MD.Ph.D., Associate Professor of

Neurosurgery, University of Udayana, Division of Neurospine, Sanglah General Hospital,

KesehatanStreet 1, Denpasar, Bali, Indonesia 80000. Phone: (62361) 249988, Fax: (62361)

244322, e-mail: tjokmahadewa@hotmail.com; tjokmahadewa@unud.ac.id

ABSTRACT

Spinal cord injury (SCI) is the cause of severe neurological disability, and no satisfactory

treatment is currently available. Evidence suggests that omega-3polyunsaturated fatty acid

(PUFA) could target some of the pathological mechanisms that underlie damage after SCI. In

parallel, promising effect of alpha-tocopherol(vitamin E) also shown as a potent antioxidant

to prevent lipid peroxidation on SCI. Therefore, we aim to investigate the combination effect

of omega-3 PUFA and vitamin E supplementation in improving motor function of weight-

dropped induced SCI in rats.

Twenty five rats were randomly divided into the five following groups: control group,

omega-3PUFA group, vitamin E group, combination treatment (omega-3 PUFAand vitamin

E) group and sham operated group. SCI was induced in the rats by using a weight-dropped

model around T9-T11 vertebra level. Omega-3PUFA and vitamin E was administered orally

on each day for two weeks of treatment. The sham group underwent laminectomy without

spinal cord compression and treatment. Motor function was measured using Basso, Beattie

and Bresnahan (BBB) locomotor scaleat the 1st, 7

th and 14

th day.We found significant

difference of motoric function between omega-3 PUFAgroup and combination treatment

group compared to the control (p<0,05). The highest BBB score on the first week was found

in combination treatment group (mean 16±2,3) but no significant difference was found

compared to the omega-3 PUFA group. On the subsequent week, combination treatment was

showing significant improvement compared to the omega-3 PUFAgroup (p<0,05).

These finding had demonstrated the effect of omega-3PUFA as neuroprotective agent to

prevent the secondary injury in SCI induced rats. Combination of omega-3 PUFAwith

vitamin E improves its efficacy to become a promising novel therapy for SCI.

Keywords: Omega-3 PUFA, vitamin E, improves motor function.

34

ULTRASOUND EVALUATION OF NEPHRITIS, HEPATIC STEATOSIS AND

KNEE JOINT EFFUSION IN BALINESE WOMEN WITH SYSTEMIC LUPUS

ERITHEMATOSUS

Elysanti Dwi Martadiani1, Andi Manaek Hatimbulan Panjaitan

2, Widyanto Pangarso

Adhi2, I Gde Raka Widiana

2, Dewa Ayu Ratna Dewi

1

1Department of Radiology, Faculty of Medicine, Udayana University, Sanglah Hospital,

Denpasar, Bali, Indonesia 2Department of Internal Medicine, Faculty of Medicine,Udayana University, Sanglah

Hospital,

Denpasar, Bali, Indonesia

ABSTRACT Introduction: Organ involvement such as nephritis, hepatic steatosis and knee joint effusion

may influence the severity of systemic lupus erithematosus (SLE). Ultrasound (US) can

detect morphological changes of those organs in SLE patients, but there is lack of data about

US-detected lupus organs involvement in Balinese women. Objective of this study is to

investigate the US findings of nephritis, hepatic steatosis and knee joint effusion in Balinese

women with SLE. Method: This study was an analytical cross sectional study. Inclusion

criteria were Balinese women, age more than 12 years, who had been diagnosed as SLE.

Exclusion criteria were heavy-drinking of alcohol, end-stage renal disease, acute knee

infection, and during US evaluation reveals hydronephrosis, renal abscess, emphysematous

pyelonephritis, kidney stone, or kidney tumor. Anamnesis, physical, laboratory and US

examination were done. The prevalence of US-detected nephritis, hepatic steatosis, and knee

joint effusion was determined. Association between several clinical and laboratory results

with US findings were tested using Spearman’s correlation and binary logistic regression

tests (α = 0.05). Result: From 57 subjects, US demonstrated nephritis in 19 (33%) subjects,

hepatic steatosis in 31 (54.5%) subjects, right knee joint effusion in 12 (21.1%) subjects and

left knee joint effusion in 14 (24.6%) subjects. No significant correlation between

dyslipidemia and hepatic steatosis (p>0.05), but there were significant correlations between

knee pain and knee joint effusion (p<0.001), also between decreased of renal function with

right and left nephritis (p<0.05). After adjustment with several laboratory indicators, only

right nephritis had significant association with decreased of renal function (p<0.05).

Conclusion: Nephritis, hepatic steatosis and knee joint effusion were identified in Balinese

women SLE patients using US. Some clinical and laboratory indicators had significant

correlation with knee joint effusion and nephritis, but most of laboratory parameters

demonstrated no significant correlation with US-detected nephritis and hepatic steatosis.

Keyword: systemic lupus erythematosus, nephritis, hepatic steatosis, knee joint effusion,

ultrasound

35

HEPCIDIN AND MATRIPTASE-2 BECOME POTENTIAL BIOMARKER FOR

RESPONSIVENESS TO ORAL IRON SUPPLEMENTATION IN FEMALE

ADOLESCENTS WITH IRON DEFICIENCY ANEMIA

Yuliana Heri Suselo, Sri Wulandari, Amelya Augusthina Ayusari, Dono Indarto

Research Group of Human Proteomics, Faculty of Medicine,

Universitas Sebelas Maret Surakarta

ABSTRACT Background. Iron deficiency anemia (IDA) is one of nutritional problems for all ages in

Indonesia. Iron fortification and supplementation has become national program for treatment

of IDA in pregnant women. The prevalence of IDA in female adolescents is also high, but

there is no program for IDA treatment. Higher levels of hepcidin are related to non-

responsiveness to oral iron supplementation. Matriptase-2 is a major enzyme which regulates

hepcidin secretion. Thus, the aim of this study was to investigate whether or not female

adolescents were responsive to oral iron supplementation. Methods. This was a quasi-

experimental study with pre- and post-test group design. Research subjects were187 female

adolescents who studied in year 10 and 11 of secondary schools in Boyolali regency.

Hemoglobin (Hb) levels were measured using the cyanmethemoglobin method and ferritin

serum used ELISA. Female adolescents with IDA were given 1 tablet ferro-fumarate per day

for 1 month. After treatment, Hb levels were determined using the same method and serum

levels of hepcidin and matriptase-2 were detected by ELISA. All data were analyzed using

independent t-test with 95% significance level. Results. Around fifty percent female

adolescents had IDA and 38.2% was responsive to iron supplementation (ΔHb ≥ 1 g/dL)

while 61.8% was non-responsive (ΔHb < 1 g/dL). Hepcidin levels of non-responsive

subjects (6.8 ng/mL) were higher than that of responsive subjects (6.4 ng/mL) but it did not

reach significant difference (p=0.30). Lower levels of matriptase-2 were significantly

observed in non-responsive subjects (666.3 pg/mL), compared with responsive subjects

(1133.0 pg/mL) (p=0.05). Conclusion. More than 50% of female adolescents is not

responsive to oral iron supplementation with higher levels of hepcidin and lower levels of

matriptase-2. Both proteins are a potential biomarker for detection of responsiveness to oral

iron supplementation.

Key words : Hepcidin, IDA, Matriptase-2, Responsiveness, Oral iron supplementation

36

THE DIFFERENCE EXPRESSION OF GLUT4 AND GLYCOGEN LEVELS ON

CELLS OF LIVER AND MUSCLE TISSUES IN HYPERGLYCEMIC AND

NORMOGLYCEMIC CONDITIONS

I Nyoman Suarsana1*

, Iwan Harjono Utama1, I Putu Gde Yudhi Arjentinia

2,

I Made Kardena3, Anak Agung Ayu Mirah Adi

3

1Laboratory of Biochemistry, Faculty of Veterinary Medicine, Udayana University, Jln. P.B.

Sudirman Denpasar, Bali, Indonesia 80233 2Laboratory of Internal Disease Veteriner, Faculty of Veterinary Medicine, Udayana

University, Jln. P.B. Sudirman Denpasar, Bali, Indonesia 80233 3Laboratory of Phatology Veteriner, Faculty of Veterinary Medicine, Udayana University,

Jln. P.B. Sudirman Denpasar, Bali, Indonesia 80233 *Correspondence to: I Nyoman Suarsana,

Laboratory of Biochemistry, Faculty of Veterinary Medicine,

Udayana University, Bali-Indonesia : suarsana65@yahoo.com; suarsana65@unud.ac.id

ABSTRACT

Background: Under normal physiological conditions, the glucose uptake into the cell is

regulated by glucose transporter (GLUT4) which is stimulated by insulin to be afterward

metabolized into energy and or stored into glycogen. The research aimed to determine the

expression of glucose transporter (GLUT4), glycogen levels on cells liver and muscle tissues

in hyperglycemic and normoglycemic conditions on rats. Method: A total of 20 rats of

Spraque Dawlly strain were grouped into 2 treatment groups. The normal group and the

hyperglycemia group. The observations were performed on: blood glucose level by glucose

oxidase biosensor method by using the Blood Glucose Test Meter of GlucoDr, liver and

muscle glycogen levels by spectrophotometry and GLUT4 analysis on cells of liver and

muscles tissues by using immunohistochemical technique. Results: The results showed that

80% of sucrose administration has caused hyperglycaemic rats with the blood glucose levels

of 131.2 g/dl. In hyperglycemic conditions, the levels of liver glycogen (9.4 g/mg) and

muscle (8.68 g/ mg) were significantly higher (P <0.05) than in the liver (8.54 g / mg) and

muscle (7.87 g /mg) in normoglycemic. Meanwhile, the liver glycogen levels were

significantly higher (P <0.05) when compared with the muscle glycogen levels in both

hyperglycemic and normoglycemic conditions. The expression of GLUT4 in hyperglycemic

was significantly higher (P <0.05) in both liver (64.05%) and muscle (56.15%) when

compared with the liver (53.21%) and the muscle (48.67% ) in normoglycemic. Between the

liver and muscle tissues, the expression of GLUT4 was significantly higher (P <0.05) in the

liver when compared with muscle tissue in both hyperglycemic and normoglycemic

conditions. Conclusion: Expression of GLUT4 and levels of glycogen in hyperglycemic liver

is higher compared to the muscles.

Keywords: glucose, glycogen, hyperglycemic, GLUT4

37

COMPARISON OF ANTIOXIDANT POTENTIAL OF ETHANOL EXTRACT WITH

AQUEOUS EXTRACT OF PURPLE SWEET POTATO TUBER IN HUMAN

VASCULAR ENDOTHELIAL CELLS EXPOSED TO H2O2

I Made Jawi1, I G K Arijana2

1.Department of Pharmacology, 2. Department of Histology,

Faculty of Medicine, Udayana University, Jl. PB. Sudirman Denpasar

Bali Indonesia.

*Corresponding author’s email :made_jawi@yahoo.co.id

ABSTRACT Oxidative stress causes endothelial dysfunction. Water extract of purple sweet potato tubers

have antioxidant potential in animal and human vascular endothelial, through increased

superoxide dismutase (SOD). This study aims to prove the comparison between ethanol

extract and aqueous extract of purple sweet potato tubers increasesing the expression of

SOD-2, SOD-3 and eNOS in human vascular endothelium. This study was an experimental

study of the vascular endothelial cells which undergo oxidative stress in vitro, which is

protected by ethanol extract or aqueous extract of purple sweet potato tuber at various

concentrations. Observance of the SOD-2, SOD-3 and eNOS in endothelial cells is done

using the method immunohyschemestry with monoclonal antibodies. The results showed an

increase in SOD-2, SOD-3 and eNOS was significantly (p <0.05) in endothelial protected by

ethanol extract or water extract of purple sweet potato tubers. The ethanol extract only

showed its effect at concentration of 1.5625 µg/ml for SOD-2 and SOD-3. The effect of

aqueous extract were at a concentration of 1.5625 to 3.125µg/ml. From these results it can

be concluded that the ethanol extract or aqueous extract of purple sweet potato tubers can

protect endothelial cells from oxidative stress through increased expression of SOD-2 and

SOD-3 .Ethanol extract requires much lower concentrations to show its effect compared with

aqueous extracts.

Keywords: Aqueous extract or ethanol extract of purple sweet potato, SOD, HUVEC.

38

THE PREVALENCE OF BACTERIAL VAGINOSIS (BV) AMONG

REPRODUCTIVE WOMEN IN BALI

Septarini NW1)

, Sutarga IM1)

, Suariyani LP1)

1)

School of Public Health, Faculty of Medicine, Udayana University, Denpasar, Bali

*Correcponding Author: septarini@unud.ac.id

ABSTRACT Bacterial Vaginosis (BV) is one of the most common vaginal infections among women in

reproductive age. This infection occurs due to the reduction of number of lactobacillus

bacteria and the massive increase of anaerobic bacteria on vagina. Gardnerella vaginalis is

one of the agents of disease. If it is left under-diagnosed, it will course serious complication

to the women, including uterus infection, pelvic inflammatory disease, sterility as well as

outside reproductive organ complication. It is also increase the chance of HIV infection. In

Bali, there was no research on the prevalence and determinants of BV. This was a cross

sectional study conducted in two STIs clinics in Bali. The samples were women in

reproductive age that visited those clinics. Besides collecting vaginal secret from the subjects

to be tested using gram stain methods to find clue cells, the subjects were also asked about

BV’s determinants. Ethical clearance was obtained from Medical Faculty/Sanglah Hospital,

Udayana University. Subjects were given informed consent prior to sample collection. The

total subjects joined this study were 100 women. The prevalence of Bacterial vaginosis

among subjects was 67%. No statistically significant relationship was found between

determinants including age, contraceptive use, parity, and condom use with the increase risk

of getting BV infection. However, the high prevalence of this disease suggest that there is a

need to screen this disease is one of the prevention matter at the general community level in

order to give prompt treatment and prevent the spread and complication.

Keywords : Bacterial vaginosis, determinants, reproductive women

39

LOW PREVALENCE OF CAVEOLIN-1 ONCOGENIC POLYMORPHISM G14713A

AND T29107A AMONG BREAST CANCER PATIENT IN SANGLAH GENERAL

HOSPITAL

Desak Made Wihandani1, Putu Anda Tusta Adiputra

2, I Gede Putu

Supadmanaba1,1Biochemistry Department Faculty of Medicine Udayana University

2Oncology Surgery Division Faculty of Medicine Udayana University/Sanglah General

Hospital

Corresponding Author: Desak Made Wihandani, Biochemistry Department Faculty of

Medicine Udayana University. Email: dmwihandani@yahoo.com

ABSTRACT

Background: Currently, breast cancer is one of the most common cancers among women,

surpassing only by cervical cancer. Despite the improvement in terms of survivability as well

as prognosis, most patients are coming with advanced disease at the time of diagnosis. Unlike

early disease, determining the outcome of advanced disease is still challenging. Initial

investigation showed that polymorphism within caveolin-1 gene could also contribute in

determining risk as well as prognosis. In our initial study, we investigated the prevalence of

two oncogenic polymorphism of Caveolin-1: SNP CAV1 G14713A and T29107A. Method:

The samples were obtained by consecutive sampling in Sanglah Hospital from January 2016

to December 2016. The blood samples were collected in EDTA tube and the DNA was

isolated using Promega Kyt. Subsequent PCR was conducted to amplify the gene which then

sequenced in Genetika Science. Result: We enrolled 43 samples with complete medical

records in our analysis. The mean age in our sample was 49.11±10.33 years and mostly

stadium III cancer (39.7%) and poorly differentiated. 68% of our sample were luminal types

and the rests were HER2 and TNBC with comparable proportion. We only detect one

heterozygous sample for G14713A polymorphism in which the patient had Luminal A type

but with high Ki67 and poorly differentiated. For T29107A, we detect two heterozygous

individuals and one homozygous individual, all with the same characteristics with G14713A.

Conclusion: The prevalence of SNP CAV1 G14713A and T29107A were considerably low in

breast cancer population in Bali. However, the characteristic of the samples with the

polymorphism suggest that further investigation is needed to confirm their effect in brest

cancer morphology.

Keywords: Breast cancer, Caveolin-1, Polymorphism, G14713A and T29107A.

40

TAENIA SPECIES IDENTIFICATION BY MPCR ON TAENIASIS PATIENT WHO

VISITED PARASITOLOGY DEPARTMENT FACULTY OF MEDICINE UDAYANA

UNIVERSITY FROM 2014 TO 2016

Kadek Swastika, Made Sudarmaja, Luh Ariwati

Department Of Parasitology Faculty of Medicine Udayana University

Corresponding Author: Kadek Swastika; Department Of Parasitology Faculty of Medicine

Udayana University. Email: kadek_swastika@unud.ac.id

ABSTRACT

Taeniasis/Cysticercosis is a zoonosis belonging to the neglected diseases. Now is still a health

problem in some developing countries. Taeniasis/cysticercosis is one of health problem in

Indonesia, Bali is one of the three endemic areas beside Papua and Nort Sumatera. Three

human Taenia species which mature in the human intestine are known in Asia. Taenia

solium, T. saginata and T. asiatica. The aim of this study was to indentify the species of

taenia by multiplex PCR and to know the prevalence of the cysticercosis among taeniasis

carriers. The progglotids/segments of the worm from the Taeniasis patient who came to

Department of Parasitology Faculty of Medicine Udayana University from 2014-2016 were

collected. Patients were treated with Yomesan® 2 gr and the proglotids/worm were collected.

These proglotids were Identified by multiplex PCR. From the taeniasis carrier also collect the

serum and confirmed by ELISA the if there were cysticercosis cases. There were total 23

worm/proglotids were collected from 23 taeniasis carrier. Patients came from four regencies

of nine regencies in Bali were : Gianyar, Badung, Denpasar and Karangasem. A total of

69.6% (16/23) patients came from Gianyar regency . There were 69,6 % patients were male

and 30,4% were female. All of proglotids were confirmed as Taenia saginata by multiplex

PCR. There were no cysticersosis cases were confirmed by ELISA among the taeniasis

carriers. The integrated health education and sustainable program to detected and deworming

carriers are important to prevent and control this disease

Keywords : Taeniasis, Bali, Cysticercosis

41

C-1562T POLYMORPHISM OF MATRIX METALLOPROTEINASE-9 (MMP-9)

GENE ASSOCIATED WITH ELEVATED LEVEL OF PLASMA MMP-9

CONCENTRATION IN PATIENT WITH ACUTE MYOCARDIAL INFRACTION

(AMI) IN DENPASAR, BALI

1Tianing Ni Wayan.,

2Bagus Ari Pradnyana Dwi.P., 1D.M Wihandani

1Department of Biochemistry Faculty of Medicine, Udayana University, Bali-Indonesia

2Faculty of Medicine Udayana-University/Sanglah Hospital, Bali Indonesia

ABSTRACT

Background: Acute Myocardial Infarction (AMI) is emergency medical condition which

still has high mortality and morbidity. In recent decades, it prevalence has a tendency to

increase, parallel with other chronic diseases. One of suspected contributing factor in AMI is

the presence of C1562T polymorphism on MMP-9 gene which increase plasma MMP-9

concentration and destabilize the plaque. However, this notion needs further confirmatory

studies, as there are several contradictive reports regarding their association. This Study

aimed to determine the relationship between C1562T polymorphism with the increase of

MMP-9 concentration in AMI. Method: A cross-sectional was conducted in Cardiovascular

Centre of Sanglah General Hospital. The peripheral blood samples were obtained from

subjects and the DNA and blood plasma was isolated. The polymorphism was detected using

PCR and RFLP while MMP-9 concentration was determined by ELISA. Results: The

average concentration of plasma MMP-9 was found to be at 8.33 ng/mL which ranged from

0.74 ng/mL until 31.93ng/mL. The proportion of MMP-9 C1562T polymorphism was 20%

from all subjects. The average concentration of MMP-9 was higher than cut-off standard

which is 0.6 ng/mL. Analysis within the group revealed that CT-genotype had significantly

higher average MMP-9 compared with CC phenotype (11.27 ng/mL vs 7.65 ng/mL).

Conclusion: C1562T polymorphism appears to be significantly associated with AMI by

increasing the concentration of MMP-9 in blood plasma.

Keywords: C1562T Polymorphism, MMP-9, Acute Myocardial Infarction

42

DETECTION OF THEA3243G MTDNA MUTATION BY PCR ALLELE SPECIFIC

AMPLIFICATION (PASA) FOR DIABETES MELLITUS TYPE 2 AT SANGLAH

HOSPITAL BALI

Surudarma W, Wihandani DM

Biochemistry Department, Medical Faculty of Udayana University

Jl. PB. Sudirman, Denpasar, Bali, Indonesia

surudarma@gmail.com

ABSTRACT

Genetic factors play a role as a cause of type 2 diabetes mellitus. Genes in mitochondrial

DNA (mtDNA) can be the cause of type 2 diabetes mellitus because oxidative

phosphorylation that produce ATP in mitochondria plays an important role in insulin

secretion by pancreatic β cells. The dysfunctionof insulin secretion is associated with the

mutation of A to G at the 3243th nucleotide position of the tRNAleu gene of mitochondrial

DNA.This mutation has been shown as a causal mutation in maternal-derived diabetes

mellitus accompanied by deafness, Maternally Inheridited Diabetes and Deafness (MIDD).

Characteristics of mutations A3243G mtDNA is a heteroplasmic mutation with a relatively

low number of mutant DNA.Several studies in Indonesia have reported the difficulty of

detecting such low frequency mtDNA heteroplasmic mutations. Therefore a precise, accurate

and relatively inexpensive method is needed. The method chosen in this study was PCR

Allele Specific Amplification (PASA)that performed on two tubes using 3 primary types,

universal primer 5'-AAC GTT GGG GCC TTT GCG TA-3 '(D1),normal primer 5'-GGG TTT

GTT AAG ATG GCA GA-3' (DN), and mutant primer 5'- GGG TTT GTT AAG ATG GCA

GG-3' (DMt). Samples that containing A3243G mtDNA mutations will produce a PCR

product with a 200 pb band on both tubesbecause heteroplasmicmutation contain of mutant

mtDNA and normal mtDNA.In this study found heteroplasmic mutation A3243G mtDNA in

21 samples of 96 patients with type 2 diabetes mellitus.

Keyword: mutation, diabetes mellitus, PASA, heteroplasmic.

43

Potential Role Of Snakes In Salmonella enteritidis And Arizona

hinshawii Transmission To Human: Zoonosis

Silvi Dwi Anggraini1 and Yos Adi Prakoso

2,*)

1Asa Animals Clinic, Kediri, Indonesia, 64117.

2Faculty of Veterinary Medicine, Gadjah Mada University, Yogyakarta, Indonesia, 55281.

*)author e-mail: yos.vet.docter@gmail.com

ABSTRACT

Salmonella enteritidis and Arizona hinshawii are common bacteria in reptiles. The bacteria

has a potency of zoonosis by human-reptiles contact, especially snakes. The aim of this study

is to isolate S. enteritidis and A. hinshawii from domesticated snakes. During 1 months of this

study a total of 37 cloacal swabs of snakes (Python reticulatus) were collected from reptiles

owner in Surabaya. The cloacal swabs were streaked on MacConkey Agar (MCA), Blood

Agar (BA) and Xylose Lysine Deoxycholate Agar (XLD) and subsequently incubated at 37o

C for 24 hours for primary isolation. Bacterial identification used an endoenzyme test

(catalase and oxidase) and Micro-ID test (Voges Proskauer, nitrate broth, phenylalanine

diaminase, H2S producing, indole, decarboxylase ornithine, decarboxylase lysine, malonate,

urease, esculin, ONPG, arabinose, adonitol, inositol, and sorbitol. The result showed that

Salmonella enteritidis (29,72%) and Arizona hinshawii (40,45%) were isolated and identified

from the collected samples. Current study supports the finding that snakes can be regarded as

critical source of S. enteritidis and A. hinshawii human infection.

Keywords: snakes, S. enteritidis, A. hinshawii, zoonosis

44

VARIOUS SYMPTOM OF CITRUS VEIN PHLOEM DEGENERATION DISEASE

(HUANGLONGBING) CAUSED BY DIFFERENT NUTRIENT DEFICIENCY AS

THE EFFECT OF Liberobacter asiaticum INFECTION

Sheila Simanjuntak, I Gede Putu Wirawan*, and Ni Made Sritamin

Laboratory of Genetic Resources and Molecular Biology

Udayana University

*Corresponding author , E-mail: putuwirawan@unud.ac.id and igpwirawan@yahoo.com

ABSTRACT Citrus vein phloem degeneration (CVPD) disease or Huanglongbing caused by a Gram

negative bacteria, L. asiaticum is the main disease of citrus plant. The symptom of the

disease very similar to the virus infection and that caused in the beginning was said as virus

infection. Recently, in 1996, it was confirmed that the disease was due to bacterial infection.

This study was done to confirm the nutrient deficiency of the citrus plants was caused by L.

asiaticum infection. Different nutrient deficiency showed different symptom of the disease

and all of those phenomena were caused by different transport protein that affect by the

infection of L. asiaticum.

Keywords: Citrus vein phloem degeneration (CVPD), L. asiaticum, deficiency.

45

LEAD DETECTION IN BLOOD OF THE BALI CATTLE THAT WERE

SLAUGHTERED IN THE TRADITIONAL SLAUGHTERHOUSES IN

DENPASAR

I Ketut Berata

1, I Made Kardena

1, Ni Nyoman Werdi Susari

2,

and I Wayan Sudira3

1Pathology Laboratory of Veterinary Medicine Faculty of Udayana University

2Anatomy Laboratory of Veterinary Medicine Faculty of Udayana University

3Farmacology & Toxicology Lab of Veterinary Medicine Faculty of Udayana University

Jl.PB Sudirman Denpasar – Bali – Indonesia

Corresponding author: berata_iketut@unud.ac.id

ABSTRACT

Contamination of lead in the body of the cattle were thought initially to be coming from the

feed and the environment in which the cattle was maintained. When a human consumes more

than 2.0 ppm of lead-contaminated beef, it could cause damage to various organs, and also

lower cognitive abilities. This study aims to determine the amount of heavy metal lead in the

blood of bali cattle which were slaughtered in a traditional slaughterhouse in Denpasar. Blood

plasma samples of 20 bali cattle were taken randomly and used for this study. The blood

stored in 10ml tubes which were filled with 0.5% Ethylene diamine tetraacetic acid (EDTA)

previously. Lead content measurement were done by using atomic absorption

spectrophotometer (AAS) method. From the measurement result, it was obtained that the lead

content average was 7.35±4.33 ppm. There was a blood sample that did not contain any lead.

The lead content of this result is higher than in the cattle at Suwung Landfill Denpasar, which

was 6.60±1.85 ppm, as the result of our research previously. This results shows that lead

contamination is not only originated from the place in which the cattle was maintained. There

were other risk factors that can contribute to it, which needs to be determined. It can be

concluded that lead contamination in blood plasma of bali cattle which were slaughtered in a

traditional slaughterhouse is 7.35±4.33 ppm. There is the need to do a further study about the

risk factors of lead contamination in the blood of bali cattle.

Keywords: lead, bali cattle, blood plasma.

46

WHOLE PLANT REGENERATION OF BALINESE RED RICE (ORYZA SATIVA

CV. BARAK CENANA) FROM MATURE EMBRYO-DERIVED CALLUS.

Ida Bagus Made Artadana1*

, Gilang Bintang Fajar Suhono1, Popy Hartatie Hardjo

1,

Maria Goretti Marianti Purwanto1, Yoko Brigitte Wang

1

1Faculty of Biotechnology, University of Surabaya, Raya Kalirungkut, 60291, Surabaya,

Indonesia. Tel.: +62-31-2981399. Fax: +61-31-2981278. *corresponding author. Email: arta@staff.ubaya.ac.id.

Abstract

An efficient regeneration is considered to be important in rice characteristic improvement

through soma clonal mutation or transformation. In this research, we aimed to optimize the

whole plant regeneration step of Oryza sativa Cv. Barak Cenana, a high value local Balinese

red rice, from previously proliferated callus.

Mature embryo-derived callus of Barak Cenana was developed into whole plants by indirect

organogenesis where the roots were firstly regenerated and then followed by shoots

formation. The optimization of Barak Cenana callus regeneration was conducted by culturing

in Murashige and Skoog (MS) basic media, supplemented with various combination of plant

growth regulators. We observed that the fastest green spot formation was resulted in MS

media containing Benzyl Adeno Purine (BAP), a cytokinine.

The highest number of regenerated plants was obtained from MS media + 5 mg/L BAP (i.e.

41 plants from 30 callus) among other concentrations of BAP used (1, 3, 5 and 6 mg/L). In

addition, we also found one albino plant derived from callus in MS media + BAP 1 mg/L

during regeneration. This character was persistent even after three times subcultures, shown

that a soma clonal mutation has been occurred.

In conclusion, we found that whole plant regeneration of Barak Cenana could be achieved by

culturing mature embryo-derived callus in MS media containing 5 mg/L BAP. Moreover, the

improvement of Barak Cenana characteristics may potentially be achieved through soma

clonal variation.

Keywords: Barak Cenana, Callus, Regeneration, BAP

47

THE POTENTIAL OF IKAN BUDU AS A LACTID ACID BACTERIA SOURCE

PRODUCING γ-AMINOBUTYRIC ACID (GABA) TO INDUCED HEAT STRESS IN

BROILER

Lili Anggraini1)

, Yetti Marlida2)

, Wizna2)

, Jamsari3)

dan Mirzah2)

1) Graduate Program Andalas University

2) Department of Nutrition and Feed Technology, Faculty of Animal Science, Andalas University

3) Department of Plant Breeding, Faculty of Agriculture, Andalas University

ABSTRACT

Ikan budu is a traditional food product made of marine fish fermented with the addition of

salt. Lactic acid bacteria (LAB) is a type of bacteria that has a critical role in the fermentation

process. The purpose of this study is to get LAB isolates that have the ability to produce γ-

Amino Butyric Acid (GABA). The study was conducted in several stages, the first was the

isolation of LAB from ikan budu based on the establishment of clear zones on MRSA media

added 2% CaCO3. Second, screening of LAB that has the capability to produce GABA by

using MRB media added 50 mM L-glutame as inducer. Third, the morphological

characterization of isolates. The result of the research showed that 9 of 17 isolates were able

to produce GABA detected through testing with TLC (Thin Layer Chromatography) use the

standards of GABA and iluen consisting of n-Butanol: acetic acid: H2O (v/v/v). Nine isolates

were subsequently named B1, B2, B3, B4, B5, B6, B7, B8 and B9. Quantitative measurement

of GABA production used a spectrophotometer with a color reagent ninhydrin and

absorbance was measured at 570 nm, with the highest yield in isolate B2 (15.365 mg / ml)

and the lowest in isolate B1 (3.765 mg / ml). The observation of morphological and

biochemical activity isolate B2 obtained that the isolates belonged to the gram-positive

bacteria, basil cell and aerobic. Based on catalase and oxidase test that showed negative

results, the isolate B2 can be classified based on the type of Lactobacillus sp. From the results

of this study concluded that LAB sourced from ikan budu has potential as a producer of

GABA which can be used as a feed additive anti heat stress for livestock, where the highest

result found in isolate B2 with production of 15.365 mg / ml.

Keywords: : LAB, GABA, ikan budu, heat stress

48

MEDICAL WASTE MANAGEMENT STRATEGY IN INPATIENT PRIMARY

HEALTH CENTER WITH SYSTEM APPROACH: A SWOT ANALYSIS

I Gusti Ngurah Gede Putra

I Gusti Ngurah Gede Putra1*

, Sang Putu Kaler Surata2, Gusti Ayu Ari Agung

2

1Master Student of Regional Planning and Environmental Management Post Graduate

University Mahasaraswati Denpasar, Indonesia 2Staf Lecturer Science Program of Regional Planning and Environmental Management Post

Graduate University Mahasaraswati Denpasar, Indonesia *Author’s email: putragusti@gmail.com

ABSTRACT Introduction: Primary health care center with inpatient services should have a proper

management of medical waste. However, this program has not been implemented properly by

any inpatient health care centers and, thus, an analysis of waste management starting from the

input, process, and output is necessary. The purpose of this study was to evaluate and analyze

the strategies of medical waste management in health care centers primarily which have an

inpatient service. Methods: A descriptive, explanatory research was conducted by using

respondents from all parties who directly involved in the management of medical waste.

Observation, questionnaires, and interviews were used to obtain the data and analysis

conducted by focus group discussions using Strength Weakness Opportunity Threats

(SWOT). Results: The Strength we found in this study include implemented medical waste

management in health care centers, sufficient funds, and adequate infrastructures. Identified

weaknesses including untrained personnel, poor planning, and poor discipline in each unit.

Opportunities that identified in our study include written regulations on an environmental

health program, the institution shaped in Public Service Board, regular training programs by

health authorities and the third parties that were available to cooperate in managing medical

waste. Several threats were identified namely environmental pollution, high demands of the

local community and the bottleneck problem in the incinerator operating licenses.

Conclusion: It is possible to implement medical waste management primary health care

center especially which have inpatient services. However, proper training, good planning,

regulation and supervision are required to ensure it continuation and maintaining the quality.

Keywords: medical waste, management, system, inpatient health care center

49

TRANSOVARIAL TRANSMISSION DENGUE VIRUS AND LARVA

DENSITY IN BALI

Sang Gede Purnama

1, Pasek Kardiwinata

1, Tri Baskoro

2

Email : sang_gede@yahoo.co.id

1. Public health department, Medicine Faculty, Udayana University

2. Parasitology department, Medicine Faculty, Gajah Mada University

ABSTRACT

Dengue hemorrhagic fever (DHF) is still a public health problem in Indonesia and it endemic in

most area of Indonesia. Previous studies were only collected the data about density of larvae

whereas rarely studies have been done on the virus virulence. This data is very important to

know the presence and types of dengue virus. Usually virus was identified by PCR analysis

which was highly cost and that caused very rare identification. Imunocytochemistry method is

cheaper than RT-PCR and also could prove their transovarian transmission from mosquito to

eggs. This study was an observational analytic with cross sectional design. Random sampling

technique was used to select 600 respondents from three regencies in Bali including 200

households in Tabanan, 200 households in Gianyar and 200 households in Denpasar. Mosquito

density was measured through a survey of entomology and Maya Index. Aedes aegypti eggs

from ovitrap was installed in the field. Mosquitoes were cultured to identify dengue virus with

immunocytochemistry methods, imunoperoksidase streptavidin-biotin complex (IISBC) on the

head squash and confirmed by RT-PCR.

Results of larva density named house index (HI), container index (CI) and breteau index (BI) in

Denpasar (11.5%; 2.6%; 13.5%), Gianyar Regency (57.5%, 10.9%; 61%), and Tabanan Regency

(33.1%; 10.4%; 42%). This results showed the evidence of transovarial transmission of dengue

virus in eggs. Transovarial index in Denpasar was 11.11%; Gianyar regency was 7.14% and

Tabanan regency was 7.14%. The positive dengue virus serotypes founded were Den-2.

There is evidence transovarial transmission of dengue virus serotypes Den-2. The density of

mosquitoes in areas outside Denpasar were high but the level of virulence of the virus in

Denpasar was the highest. It could became a determinant factor that increases the number of

dengue cases.

Keywords: transovarial, dengue, Bali

50

THE POTENTIAL OF TAPE SINGKONG AS LACTIC ACID BACTERIA PRODUCING

GLUTAMATE TO INCREASE QUALITY OF CARCASS BROILER

Vabera Maslami1)

, Yetti Marlida2)

, Mirnawati2)

, Jamsari3)

and YuliatyShafan Nur2)

1) Undergraduate Program,Andalas University

2) Department of Animal Nutrition and Feed Technology, Faculty of Animal science, Andalas University

3) Department of Plant Breeding, Faculty of Agriculture, Andalas University

ABSTRACT Glutamate acid is the amino acid building blocks of other amino acids, neurotransmitters and

forming the image of taste. Lactic Acid Bacteria (LAB) is one of the glutamate acid-producing

microorganisms are widely found in fermented products. Tape singkong is one of the traditional

foods produced from the fermentation of cassava with the activity of several types of

microorganisms. The study consisted of three stages. Stage 1; isolation LAB Tape singkong from

Padang and Bukit Tinggi . Stage 2; Qualitative and quantitative selection of LAB which

producing glutamate acid. Stage 3; Characterization the best LAB in producing acid glutamate.

The results was obtained 68 isolates of LAB from Padang and 54 Isolates of LAB from Bukit

Tinggi were then selected qualitatively using TLC (then layer chromatographic) were immersed

in a solvent mixture of n-buthanol, acetic acid and water (4: 1: 1 v / v) that 60 isolates of LAB

from Padang and 26 isolates of LAB from Bukit Tinggi producing glutamate acid. After the

selection the performed qualitative quantitative selection with spectrophotometer was measured

at 570nm. Highest Glutamate acid production is isolates from Padang TS.P 15 22.58 mg / ml

and isolates from Bukit Tinggi TS.B 16 16.97 mg/ml. the Two isolates which producing the

most high Glutamate acid Characterization according the organisms by reference to Bergey’s

manual of systemic bacteriology. The characterization of two isolates (TS.P 15 and TS.B 15)

was bacill, convex surface, white milk, and was a gram positive bacteria and aerobic. Based on

catalase test and oxidase test showed that isolate TS.P 15 and TS.B 15 was negative catalase and

oxidase, but for the glucose, sucrose and mannitol test the two isolates were positives and

negatives to lactose test. Based on the characterization, the two isolates were Lactobacillus sp.

The conclusion from this study is there isolates Isolates of LAB TS.P16 (Lactobacillus. Sp)

which potential to produce glutamate acid with the production of 22.58 mg / ml.

Keywords: Glutamate acid, LAB, Tape Singkong

51

POSTER PRESENTATION

52

ANALYSIS OF FOOD USAGE AND NUTRITIONAL STATUS IN RURAL AREA IN

BALI PROVINCE, INDONESIA

Kadek Tresna Adhi1, a)

, Ni Wayan Arya Utami1, b)

, I Made Suka Adnyana1, c)

, Ni Wayan

Septarini1, d)

, Ni Putu Ayu Wulan Noviyanti1, e)

1Fakultas Kedokteran, Universitas Udayana

a)

ktresnaadhi@unud.ac.id b)

arya_utami@yahoo.com c)

madesukaadnyana@yahoo.com d)

septa_rn@yahoo.com e)

wulann98@gmail.com

ABSTRAK

Report of Food and Nutritional Awareness System in 2012 revealed that the condition of food

usage in Bali Province of Indonesia was classified as vulnerable especially in the poor area. This

research aimed to understand the detail condition of food usage and nutritional status in the poor

area of Bali Province. The design of this research was a descriptive cross-sectional observational

study with the qualitative and quantitative approach. The sample of quantitative research was

targeted family. Meanwhile, qualitative research evaluates the opinion from health care

providers, community leaders, and cadres from selected village in Karangasem, Klungkung,

Bangli, and Buleleng district. The data was analyzed descriptively to obtain the proportion of

food usage and nutritional status. The result revealed that there was 75.5% family with

vulnerable food status (VFS), 11.1% with prone food status (PFS) and 1.4% with deficient food

status (DFS). Furthermore, there were 16.4%. To conclude, the region from which the samples

were obtained was in the state of food vulnerability which resulted in the emergence of various

nutritional problem among the children.The active role of government and local community are

needed to optimize the utilization of local food sources to improve the nutritional status of the

local community especially toddlers and children.

Keywords: food utilization, nutritional status, food vulnerability, toddler, and poor area

53

THE RISK OF CERVICAL ADENOCARCINOMA IN PATIENTS INFECTED

WITH HUMAN PAPILLOMAVIRUS TYPE-18

I Gusti Ayu Sri Mahendra Dewi

Department of Pathology Anatomy

Faculty of Medicine Udayana University/ Sanglah Hospital Denpasar Bali

ABSTRACT

Introduction: cancer is still a health problem worldwide. Cervical cancer is the second most

common cancer in women in developing countries. High-risk Human Papillomavirus (HPV) is

well recognized as the causative agent of cervical cancer. Squamous cell carcinoma and

adenocarcinoma are the most and the second most common histopathological type. HPV

genotypes probably correlate to histopathological type. This study aim to prove that infection by

HPV-18 give a greater risk for the occurrence of cervical adenocarcinoma compared with

infection by other high risk HPV types. Methods: This study was case control study.

Adenocarcinoma with HPV-18 positive as the case group and adenocarcinoma positive with

other high risk HPV types as the control group. Histopathological diagnosis performed on H & E

stain. Genotyping of HPV used SPF-10 and specific E7-primer by LiPA. Bivariate analysis was

employed to calculate odds ratios (ORs) and Pearson’s X2 test was applied. Result: Total

number of adenocarcinoma observed in this study were 42 patients, consisted of 12 cases and 30

controls. The most prevalence adenocarcinoma occurred in the age group 41-50 years. The risk

of adenocarcinoma in patients with HPV-18 infection was 2.88 fold compared with infection by

other high risk HPV types (95% CI = 1.12-7.38; p = 0.024). Conclusion: HPV-18 infection

caused cervical adenocarcinoma is 3.0 more than other high risk HPV types

Keywords: cervical adenocarcinoma, HPV-18, histopathological type

54

THE PROFILE OF EPIDERMAL GROWTH FACTOR RECEPTOR MUTATION

DETECTED FROM CYTOLOGIC SLIDES OF LUNG ADENO CARCINOMA

PATIENTS IN SANGLAH HOSPITAL, BALI

Ni Wayan Winarti, Herman Saputra

Department of Pathology Anatomy, Faculty of Medicine,

Udayana University/Sanglah General Hospital, Bali, Indonesia

ABSTRACT

Introduction: Epidermal Growth Factor Receptor (EGFR), KRAS and ALK are prototypical

driver mutation which play fundamental roles in lung tumorigenesis. Improper activation of

EGFR due to somatic EGFR gene mutation, may result in increased cell survival, proliferation,

invasion and metastasis in lung adeno carcinoma. Here in we determined the profile of EGFR

mutation in lung adeno carcinoma using cytologic slides, that may serve useful information in

the management of lung cancer by targeting approach of anti EGFR. Methods: This is a cross

sectional study included all lung adeno carcinoma cases in Sanglah Hospital, Denpasar, who

underwent EGFR mutation examination during the year 2013-2016. The diagnosis and EGFR

mutation examination both using cytologic slides. The frequency and types of EGFR mutation

were collected and compared between age and sex. The result then showed descriptively and

analyzed with chi square test. Result: One hundred and nine cases were included in this study,

consisted of 56% (61 cases) male and 44% (48 cases) female, ranging from 32 until 91 year old.

The peak ages were 61-70 year old (30.3%) and 51-60 year old (29.4%). EGFR examination

revealed 51.4% (56 cases) showed mutation and 48.6% (53 cases) were wild type. Three most

common types of mutation with highest sensitivity to anti EGFR were obtained in this study.

They were Ins/Del exon 19 (25.7%), point mutation of Exon 21 L858R (16.5%) and Exon 21

L861Q (5.5%). The remainder, mutation of Exon 20 T790M (0.9%) and mixed mutation (2.8%),

were considered resistent to anti EGFR. The mutation was more frequent in female (66.7%) than

male (39.3%), but not significanly different between age ≤ 50 (54.8%) and > 50 year old (50%).

Conclusion: In order to improve the management of patients with lung adeno carcinoma, the

detection of EGFR mutation from cytologic slide is valuable.

Keywords: lung adeno carcinoma, cytology, EGFR mutation

55

COMBINATION OF HIGHER DOSE METFORMIN AND LOWER DOSE CISPLATIN

INCREASES APOPTOSIS IN CERVICAL CANCER CELLS LINE

Ratih Dewi Yudhani1, Muthmainah

2, Dono Indarto

3

1 Departement of Pharmacology, Medical Faculty, Universitas Sebelas Maret, Surakarta,

Indonesia 2

Departement of Anatomy, Medical Faculty, Universitas Sebelas Maret, Surakarta,

Indonesia 3

Departementof Physiology, Medical Faculty, Universitas Sebelas Maret, Surakarta,

Indonesia

ABSTRACT

Introduction:Cervical cancer is one of the highest gynecologycal malignancy worldwide

including Indonesia which leads to high morbidity and mortality rate. Cisplatin is the first line

chemotherapy for treatment of cervical cancer but its use is limited because of serious side

effects. Our previous study has also showed that metformin enhances the anti-proliferative effect

of cisplatin in cervical cancer cell line (HeLa). This study aimed to investigate whether or not

HeLa cells treated with metformin and cisplatin enhances cell apoptosis. Methods:2 x 105

HeLa

cells were cultured with RPMI medium supplemented with 10% fetal bovine serum, 2%

penicillin-streptomycin and 0.5% fungizone for 24 hours. HeLa cells were then treated with 60

mM metformin, 40 µM cisplatin or combination of 30 mM metformin and 6.25 µM cisplatin or

7.5 mM metformin and 12.5 µM cisplatin for 48 hours. Apoptotic and necrotic cells were

measured using flow cytometer. All data were statistically analyzed using Kruskall-Wallis

testand the significant value was set up < 0.05. Results:HeLa cells which were treated with 30

mM metformin and 6.25 µM cisplatin had higher apoptosis (10.26 ± 7.72 %) than HeLa cells

without treatment (2.72 ± 2.30 %) but it was not significantly difference (p = 0.26). Mean while,

7.5 mM metformin and 12.5 µM cisplatin administered to HeLa cells decreased apoptosis (4.73

± 1.32%), compared with apoptosis in the earlier combined dose. Unfortunately, apoptosis

induction of these drug combination was much lower than HeLa cells treated with 60 mM

metformin (30.32 ± 22.54%) or 40 µM cisplatin (14.80 ± 3.61%) alone. However, the different

percentage of apoptosis did not reach significance (p = 0.26). Conclusion: Administration of 30

mM metformin and 6.25 µM cisplatin in HeLa cells induces higher apoptosis than that of 7.5

mM metformin and 12.5 µM cisplatin.

Keywords: Metformin, Cisplatin, Apoptosis, HeLa cells

56

S-GENE MUTATIONS OF HEPATITIS B VIRUS IN HEPATITIS B CHRONIC

PATIENTS AFTER 12 WEEKS OF TELBIVUDINE TREATMENT

Yenti Purnamasari1, Susan Irawaty Ie

2, Luthfi Parewangi

3, Nasrum Massi

4, David H.Muljono

2,4,5

1Medicine Faculty of Halu Oleo University, Kendari, Indonesia; 2Eijkman Institute for Molecular Biology, Jakarta, Indonesia;

3Gastrohepatology Department of WahidinSudirohusodo Hospital, Makassar, Indonesia;

4Medicine Faculty of Hasanuddin University, Makassar, Indonesia;

5Sydney Medical School, University of Sydney, Sydney, Australia

Email: yenti.purnamasari@ymail.com

ABSTRACT Background: Hepatitis B is an inflammation of liver cells caused by hepatitis B virus (HBV). In

Indonesia, the average prevalence of HBV surface antigen (HBsAg) is 9.4%, categorizing

Indonesia as an endemic area. Telbivudine antiviral therapy aims to suppress HBV replication

targeted on polymerase region, unless in the presence of drug-resistant mutations, the mutant will

replicate continuously. On the other hand, the overlapping structure of the HBV genome means

that alterations in the P gene will affect S gene as well. This study aimed to observe the

mutations in HBsAg and predict the antigenicity index changes of HBsAg mutants in chronic

hepatitis B patients after 12 weeks of Telbivudine treatment. Methods: Serum samples of 26

chronic hepatitis B subjects who have received 12 weeks of Telbivudine treatment in

Gastrohepatology Department of Wahidin Sudirohusodo Public Hospital were collected as

samples of this study. HBV DNA was extracted and amplified with nested polymerase chain

reaction (PCR) method. Positive amplification results were purified, sequenced and analyzed for

the presence of mutations. Result: Overall, there were 21 point mutations were found in HBsAg

from 13 positive samples, one of which is associated with diagnostic failure and escape mutants

of active vaccination, while 2 point mutations are associated with HBIg passive vaccination

escape mutants. Seven mutations found in the ‘a’ determinant of HBsAg, with 4 point mutations

occured within the 1st loop of 'a' determinant showing the highest antigenicity index changes,

which may cause alterations of the immunological properties of the HBsAg mutants.

Keywords: Hepatitis B Virus, S Gene, chronic hepatitis B, telbivudine treatment, week 12

57

ETHANOLIC EXTRACT OF THE POWDER OF RED EARTHWORM (Lumbricus

rubellus) OBTAINED FROM SEVERAL ORGANIC FARMLANDS IN BALI,

INDONESIA: ANALYSIS OF TOTAL PHENOLIC CONTENT AND ANTIOXIDANT

CAPACITY

Ni Wayan Sucindra Dewi,1 Agung Nova Mahendra,

1,2 Gustu Widi Kencana Putra,

3

I Made Jawi,1 Dewa Made Sukrama,

4 Ni Luh Kartini

5

Department of Pharmacology and Therapy,1 Division of Drug Development and Laboratory

Animal, Integrated Biomedical Laboratory Unit, Faculty of Medicine,2 Laboratory of Food

Analysis, Faculty of Agricultural Technology,3 Department of Microbiology, Faculty of

Medicine,4 Faculty of Agriculture,

5 Udayana University, Bali, Indonesia.

ABSTRACT

Background: Red earthworm (Lumbricus rubellus) has been used for centuries as an ingredient

of Ayurvedic therapy and traditional Chinese medicine (TCM). The worm is used in the

treatment of inflammation, hematological disorders, fever, hepatic disorders, joint pain, high

blood pressure, and several other indications. L. rubellus is also bred and developed into powder

in Bali, Indonesia to be used empirically in the treatment of diabetes, stroke, and joint pain.

These disorders are known to be associated with inflammation and oxidative stress. Phenolics are

known to act as anti-inflammatory agent. To our knowledge, there is no data regarding the total

phenolic content and antioxidant capacity of L. rubellus obtained from Bali. Methods: Ethanolic

extract of L. rubellus powder was made in The Laboratory of Division of Drug Development and

Laboratory Animal, Integrated Biomedical Unit, Faculty of Medicine, Udayana University. Total

phenolic content and antioxidant capacity were investigated in The Laboratory of Food Analysis,

Udayana University. Total phenolic content was determined using Folin-Ciocalteu method,

meanwhile antioxidant capacity was determined using DPPH method. Results: We found that

the ethanolic extract of L. rubellus powder had total phenolic content of 1016.31 mg/100 g gallic

acid equivalent (GAE) and exhibited IC 50% of 12.33 mg/mL. Conclusion: We conclude that

ethanolic extract of L. rubellus powder obtained from several organic farmlands in Bali contains

phenolic acid and shows antioxidant effect in vitro. L. rubellus powder can be potentially used as

a natural antioxidant source to treat disorders associated with inflammation and oxidative stress.

Keywords: Lumbricus rubellus powder, ethanolic extract, total phenolics, antioxidant capacity

58

CYP2E1 GENOTYPE AND TRANSAMINASE LEVEL OF TUBERCULOSIS PATIENTS

RECEIVING FIXED DOSE COMBINATION OF ANTITUBERCULOSIS

I Gusti Ayu Artini1, I Gusti Ngurah Bagus Artana

2, I Gusti Made Aman

1, Agung Nova

Mahendra1

1Pharmacology Department, Medical Faculty, Udayana University, Indonesia

2Internal Medicine Department, Medical Faculty, Udayana University, Indonesia

iga_artini@yahoo.com

ABSTRACT

Introductions: Antituberculosis drug-induced liver injury (ATLI) had become a common

serious side effect regarding antituberculosis use. Isoniazid (INH) was believed as a crucial

factor related to ATLI incidence and a genetic factor related to INH metabolism (eg. CYP2E1)

was assumed as an important contributor of ATLI. This study aimed to investigate the genotype

pattern of CYP2E1 and serum transaminase level on tuberculosis patients receiving fixed-dose

combination of antituberculosis. Methods: As many as 35 tuberculosis patients attending

Pulmonary Outpatient Clinic of Sanglah Hospital were included in this cross-sectional study.

Identification of CYP2E1 genotype was performed with PCR-RFLP assay using RsaI and DraI

restriction enzyme. Results: This study revealed the proportion of c1/c1; c1/c2; and c2/c2

genotype of CYP2E1 on 5’-flanking region were 62.9%; 34.3%; and 2.8%, respectively; whereas

the proportion of DD, CD and CC genotype of CYP2E1 on intron 6 were 60%; 28.6%; and

11.4%, respectively. The proportion of hepatotoxicity was 14.3%, while the mean value of AST

and ALT level were 23.5±13.6 IU/L and 23.3±21.1 IU/L. Conclusion: The dominant proportion

of CYP2E1 genotype on 5’-flanking region and intron 6 were c1/c1 and DD.

Keyword: antituberculosis drug-induced liver injury, isoniazid, CYP2E1 genotype

59

PHARMACO-ETHOLOGICAL AND HIPPOCAMPAL CA1 REGION

NEUROHISTOLOGICAL STUDY OF SERTRALINE EFFECTS ON SINGLE-

ROLONGED STRESS-INDUCED RODENT MODEL OF PTSD

Agung Nova Mahendra,1,2*

I Nyoman Tri Pramartha,3 Ni Luh Sari Darmayanti,

3

Ni Wayan Sucindra Dewi1

Department of Pharmacology and Therapy,1 and Division of Drug Development and Laboratory

Animal, Integrated Biomedical Laboratory Unit,2 and Undergraduate Student of Physician

Education Study Program (PSPD),3 Faculty of Medicine, Udayana University, Bali, Indonesia

*Correspondence: Agung Nova Mahendra, MD, M.Sc.

Faculty of Medicine, Udayana University, Bali, Indonesia

E-mail: novamahendra@unud.ac.id

ABSTRACT Background: Sertraline is one of several drugs approved for posttraumatic stress disorder

(PTSD), an anxiety disorder which is often accompanied by hippocampal defect. Hippocampal

CA1 region is known to be associated with anxiety. Single-prolonged stress (SPS)-treated rats, a

rodent model of PTSD, has been found to induce apoptosis of hippocampal pyramidal neurons.

To our knowledge, the effect of sertraline on anxiety-like behaviors and hippocampal CA1

neurons had not been studied in SPS-induced rats as a model of PTSD. The aim of this study was

to investigate the effect of sertraline on classical and ethological variables of anxiety-like

behavior, and histomorphological appearance of hippocampal CA1 region in SPS-induced rats.

Methods: Wistar male rats (8-10 weeks; 150-180 g [n = 35]), were randomized into 5 groups.

Group 1: Normal control. Group 2: Rats induced with SPS for 7 days + 7 days of consolidation.

Group 3: Rats induced with SPS for 7 days + 7 days of consolidation + sertraline 4.5 mg/kg/day

(orally). Group 4: Rats induced with SPS for 7 days + 7 days of consolidation + sertraline 9

mg/kg/day (orally). Group 5: Rats induced with SPS for 7 days + 7 days of consolidation +

sertraline 18 mg/kg/day (orally). Sertraline was administered for 14 days. Behavioral measures

were studied with elevated plus maze test. Hippocampus-containing coronal slices of the brains

were HE-stained and analyzed using Optilab. Results: The numbers of closed arm entries and

stretched-attend posture (SAP) were not different between groups. Sertraline decreased time

spent in closed arms, increased open arm entries, time spent in open arms, and unprotected head

dips (UHD). Lowest dose of sertraline exerted highest level of anxiety as evidenced by the

number of protected head dips (PHD), but higher doses ameliorated this effect. Spatial

arrangement of CA1 pyramidal neurons was severely impaired in all SPS-exposed groups.

Pyknotic, dark-stained, and shrinked neurons were commonly found in sertraline-treated rats,

especially at the dose of 18 mg/kg/day. Conclusions: We found that chronic administration of

sertraline exerts anxiolytic effect on single-prolonged stress-induced rats. Increasing dose of

sertraline is associated with increasing “chaotic” appearance of hippocampal CA1 region

histomorphology.

Keywords: Sertraline, pharmaco-ethological analysis, hippocampal CA1 region, single-

prolonged stress, rats

60

DETERMINANTS OF POST TRAUMATIC STRESS DISORDER (PTSD)

AMONG TRAFFIC ACCIDENT VICTIMS IN BALI – INDONESIA

Putu Aryani1, Cokorda Bagus Jaya Lesmana2, Pande Putu Januraga3,

Komang Ayu Kartika Sari1, Nyoman Sutarsa1

1Department of Community and Preventive Medicine, Faculty of Medicine, Udayana University, 2 Department of Psychiatric,

Faculty of Medicine, Udayana University, 3School of Public Health, Faculty of Medicine, Udayana University

ABSTRACT

Background: The incidence of traffic accident in Bali has increased over the last decade. The

mortality rate remains high – contributing to more than 500 deaths annually between 2011 and

2013. Studies have revealed that traffic accident victims are more vulnerable to experience post-

traumatic stress disorder (PTSD). This however is rarely been explored in the context of eastern

culture society especially among the Balinese. This study was aimed at exploring the incidence

and determinants of PTSD among traffic accident victims in Bali Province. Methods: A cross

sectional analytic study was conducted to explore incidence and severity of PTSD among traffic

accident victims in Bali Province between 2015 and 2016. A total of 153 respondents were

selected using an accidental technique. Data were collected using a structured interview guided

by structured questionnaires. The international questionnaire (PCL-C V) to screen the symptom

of PTSD for traffic accident victims was adopted. Data were analyzed by applying statistical

analyses. Results: The majority of respondents were 18-27 years (65.4%), female (62.1%),

Balinese (87.6%), Hindu (85.6%), being single (64.1%), high school graduated (50.3%),

employed (62.2%), and living with the extended family (42.5%). Prevalence of PTSD among

traffic accident victims was only 1.9%. It might be associated to the late screening time where

the majority of respondents were interviewed a month or more after the accident (71.5%). As

many as 46.2% respondents reported a mild symptom of PTSD. Higher proportion was found

among younger age, female, low educational level, severe injuries and longer hospitalization.

Conclusion: The prevalence of PTSD among traffic accident victims was very low which could

be underestimated due to the late screening process. An early screening and a prompt treatment

are required to facilitate coping strategies among traumatic victims.

61

SUPPLEMENTATION 2000MG AND 1000MG OF LINOLEIC ACID AND

ALFA LINOLENIC ACID DELAYED PREDIABETIC STATE IN

BALINESE YOUNG OBESE WOMEN: A RANDOMISED CLINICAL

TRIAL I W Weta

1, Wayan P Sutirtayasa

2, Anak Agung N Subawa

3, Safarina G Malik

4.

1 Department of Community and Preventive Medicine/Department of Clinical Nutrition, Facullty of Medecine,

Udayana University/Sanglah Hospital, Denpasar Bali Indonesia. 2

Study Program Doctor, Post Graduate Program Faculty of Medicine Udayana University, Denpasar Bali

Indonesia. 3 Department of Clinical Pathology Faculty of Medicine Udayana University Denpasar Bali Indonesia.

4 Eijkman Institute for Molecular Biology, Ministry of Research Technology and Higher Education, Republic of

Indonesia, Jakarta, Indonesia

Corresponding Author: Dr I Wayan Weta, Department of Preventive and Community Medicine/Clinical Nutrition,

Faculty of Medicine, Udayana University/Sanglah Hospital, Jl. PB Sudirman, Denpasar, Bali 80232, Indonesia.

Tel: +62 81337005360; Fax: +620361246656

Email: wy_weta@unud.ac.id

ABSTRACT

Background. Obesity fuelled epidemic of type 2 diabetes mellitus, globally. Controlling

prediabetic state in obese person is important to prevent the type 2 diabetes. PUFAs (n-6 and n-

3) have a role in controlling insulin resistance. The aim is to elucidate the effect of the linoleic

acid (LA) and alfa linolenic acid (ALA) supplementation to fasting plasma glucose in young

women obese patients. Material and Method. In totally, 45 young obese women in Denpasar

Bali, randomized into two group; 22 participants were supplemented with 2000mg and 1000mg

of LA and ALA (Intervention), and 23 participants were given placebo (Control), for 12 weeks.

Both groups were recommended to restrict their daily energy intake <1500 kcal, during the

study. The data namely; dietary, anthropomeric, blood triglyceride, plasma fasting glucose were

assessed in base-line and end-line of study. Results. Both groups resticted their energy intake

less than 1500 kcal in the endline assessment. All of body fat parameters (BMI, triglycreide,

waist circumference, conisity index, waist to height ratio, and lipid accumulation product;

p=0.003, p=0.049, p<0.001, p=0.002, p=0.001, and p= 0.001, respectively) decreased in the

Intervention group. However, only the conisity index decreased (p=0.025) in the Control group.

In the Intervention group no significant change of fasting plasma glukose, contrary it increased

(p=0.007) in the Control group. The impaired plasma glucose increased in the Control group

(OR=8, p=0.039), but not in the Intervention group (p=0.508). Conclusion. Restriction energy

intake with supplementation 2000mg and 1000mg of LA and ALA control prediabetes of young

obese women.

Key words: alfa linolenic acid, fasting plasma glukose, prediabetes, young obese women.

62

THE ANTICANCER ACTIVITY OF (E)-1-(4’-AMINOPHENYL)-3-PHENYLPROP-2-

EN-1-ON AGAINST DMBA-INDUCED MAMMARY CANCER IN SPRAGUE DAWLEY

RAT THROUGH THE REGULATION OF MICRORNA-21 EXPRESSION

Ida Ayu Ika Wahyuniari1, I G K Nyoman Arijana

1, Ni Putu Sriwidyani

2, Ida Ayu Dewi

Wiryanthini3, Mustofa

4, Muhammad Ghufron

5, Sitarina Widyarini

6, Sofia Mubarika

5

1Department of Histology, Faculty of Medicine, Udayana University, Bali,

2Department of Anatomical Pathology, Faculty of Medicine, Udayana University, Bali,

3Department of Biochemistry, Faculty of Medicine, Udayana University, Bali,

4Department of Pharmacology and Therapy, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta,

5Department of Histology and Cell Biology, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta,

6Department of Anatomical Pathology, Faculty of Veterinary Science, Universitas Gadjah Mada,

Yogyakarta, Indonesia.

Correspondence to Ida Ayu Ika Wahyuniari, Department of Histology, Faculty of Medicine, Udayana

University, Jalan PB. Sudirman, Denpasar, Bali, 80237, Indonesia

email : ikawahyuniari@yahoo.com

ABSTRACT

Background: The new anticancer is urgently needed due to the high resistance and recurrence of

breast cancer. Previous study reported that a new chalcone derivative, (e)-1-(4’-aminophenyl)-3-

phenylprop-2-en-1-on, has a potential cytotoxicity against T47D breast cancer cell line. In this

study we investigated the anticancer activity of (e)-1-(4’-aminophenyl)-3-phenylprop-2-en-1-on

against DMBA-induced mammary cancer in Sprague Dawley rat and its effect on microRNA-21

expression. Methods: Twenty four female rat were divided into 6 groups. The first group, G1 as

negative control. The groups 2 to 6 (G2, G3, T1, T2, and T3) were induced by DMBA 20

mg/kgBW for 5 weeks. After breast nodule observed, G2 as vehicle-treated group and G3 as

positive control received tamoxifen, respectively. Whereas, T1, T2, T3 received (e)-1-(4’-

aminophenyl)-3-phenylprop-2-en-1-on with different doses, ie. 5, 15, and 45 mg/kgBW/day for

21 days, respectively. Tumor size was measured every weeks for 21 days and on day 22, plasma

and breast tissues were collected to examine the miR-21 expression by qRT-PCR and

histopathological feature, respectively. Result: The result showed that significantly decreased

tumor growth (p<0.05) and better histopathological malignant grading in T1, T2, T3 were

observed. Moreover, significantly reduced miR-21 relative expression (p<0.05) in T1 and T2

were also observed. Conclusion: (e)-1-(4’-aminophenyl)-3-phenylprop-2-en-1-on has potential

anticancer activity on DMBA-induced mammary cancer in Sprague Dawley rat through its

activity on miR-21 expression. Hence, (e)-1-(4’-aminophenyl)-3-phenylprop-2-en-1-on might be

a new anticancer candidate in the future.

Keywords: (e)-1-(4’-aminophenyl)-3-phenylprop-2-en-1-on, tumor growth, histopathology,

microRNA-21 expression, breast cancer

63

PREPARATION AND IN VITRO CHARACTERIZATION OF SELF-NANO

EMULSIFYING SYSTEM OF C- PHENYLCALIX-[4]-RESORCINARYL

OCTACINNAMATE AND C-METHYLCALIX-[4]-RESORCINARYL

OCTABENZOATE AS ULTRAVIOLET ABSORBERS

Indrayani Agung Wiwiek1, Suwaldi Martodihardjo

2, Soenardi

3, Jumina

4, I Gusti M

Ngurah Budiana5, Mustofa

6

1Department of Pharmacology, Faculty of Medicine, Udayana University, PB.Sudirman, Bali 80237, Indonesia

2Department of Pharmaceutics, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281

3Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Sekip Utara,

Yogyakarta 55281 4Department of Skin and Venerelogy, Faculty of Medicine, Universitas Gadjah Mada, Bulaksumur Yogyakarta

55281, Indonesia 5Departement of Chemistry, Faculty of Education and Teacher Training, Nusa Cendana University, Kupang 85001,

Indonesia 6Department of Pharmacology and Therapy, Faculty of Medicine, Universitas Gadjah Mada, Bulaksumur

Yogyakarta 55281, Indonesia

Address of Correspondence: Agung Wiwiek Indrayani Department of Pharmacology, Faculty of Medicine,

Universitas Udayana, Jl. PB.Sudirman Bali 80237, Indonesia Email: agungwiwiekfk@gmail.com

ABSTRACT

Background: Sunscreens are cosmetic products which are used to protect the skin from

ultraviolet (UV) rays exposure. Ultraviolet rays, especially ultraviolet B (UVB) rays can cause

skin cancer. C-phenylcalix-[4]-resorcinaryl octacinnamate (compound 1) and C-methylcalix-[4]-

resorcinaryl octabenzoate (compound 2) are newly synthesized compounds which have high

absorbance of UVB rays. Despite their potential activity, these compounds have low solubility in

water. Therefore, in order to increase the solubility, they were formulated into a nanoemulsion.

Objective: The aim of the present study was to prepare and characterize a self-nano emulsifying

system of two compounds as UV absorber. Methods: Optimum formula of nanoemulsion was

analyzed using D-optimal mixture design with Design Expert® ver. 7.1.5 by varying

concentrations of candlenut oil, tween 80, and polyethylen glycol 400 (v/v). Sun Protection

Factor (SPF), transmittance and time of emulsification were evaluated as optimization

parameters. The optimal formula was further evaluated for its distribution, droplet size, and zeta

potential. Results: A combination of two compounds was used in formulations and has SPF

value of 52.189. The composition of optimal formula was obtained at 2.5 mL of candlenut oil,

5.066 mL of tween 80, and 2.434 mL of polyethylene glycol 400. The results showed that the

optimal formula has SPF value of 81.577, % of transmittance 90.84, emulsification time of 17.42

seconds, desirability of 0.726, average droplet size of 129.9 nm with polydispersity index 0.393

and zeta potential of -15.2 mV. Conclusion: Nanoemulsion formulation using candlenut oil

could increase SPF value of sunscreen compounds and potentially be used as alternative UV

absorbers dosage forms in the future.

Keywords: self-nanoemulsifying system, formulation, C-phenylcalix-[4]-resorcinaryl

octacinnamate, C-methylcalix -[4]-resorcinaryl octabenzoate, UV absorbers, candlenut oil.

64

DETECTION OF CITRUS VEIN PHLOEM DEGENERATION (CVPD)

DISEASE AND THE QUALITY OF HEALTHY FRUITS IN NUTRIENT

DEFICIENCY OF CITRUS

I Gede Putu Wirawan*, Sheila Simanjuntak, Made Sritamin, and

Nyoman Wijaya

Graduate School of Agricultural Biotechnology, Faculty of Agriculture, Udayana University

Jalan. PB. Sudirman. Denpasar 80232 Bali. Email: igpwirawan@unud.ac.id

ABSTRACT

Background: Citrus plantation has a main disease called citrus greening disease or

Huanglongbing or Citrus Vein Phloem Degeneration (CVPD), and in Indonesia is wellknown as

CVPD disease. The disease caused by Gram negative bacteria called Liberobacter asiaticus. The

disease symptom is very similar to plant deficiency of nuitrients and can be confirmed by using

polymerase chain reaction (PCR). Objective: This study try to find the nutrients deficiency,

quality of the fruits, and to detect the present of the pathogenic bacteria, L. asiaticus. Methods:

In the study of nutrients deviciency was done by using AAS technique, while the study of the

healthy and consumable fruits was done by using AAS and HPLC techniques. Detection of L.

asiaticus we used the PCR method. Results: The results of this study showed that all citrus

plants showing the nutrients deficiency were infected by the pathogenic bacteria. Nutrient

deficiency of citrus plants were found to be deficiency in Fe, Mn, Mg, or Zn and the quality of

the fruits therefore, were physically small and lack of vitamin C and antioxidances. Conclusion:

The results indicated that there is a correlation between the citrus plant’s infected by L. asiaticus

caused nutrients deficiency and lead to the quality of the fruits that we consume.

Keywords: Citrus plants, nutrient deficiency, Liberibacter asiaticus, PCR, AAS

65

MUTATION IDENTIFICATION OF RPOB GENE FROM MDR-TB ISOLATES AT

SANGLAH HOSPITAL DENPASAR, BALI

Dwija, Ida Bagus Nyoman Putra, Ni Made Adi Tarini, Ida Ayu Kade Ratna

Sukmaningsih, Wahyu Hidayati

Microbiology Department, Faculty of Medicine Udayana University, Bali.

Corresponding author: p.dwija@Unud.ac.id

ABSTRACT

Tuberculosis (Tb) that caused by Mycobacterium tuberculosis (Mtb) bacteria is one of the

leading infections that caused high mortality rate annually. Anti-mycobacterium drugs is almost

successful to control the burden of diseases, but emergence of drug resistance bacteria such as

Multi Drug Resistance (MDR-) or Extended Drug Resistance ( XDR-) tuberculosis is an another

problem faced today. Genetic change or mutation is one of the mechanisms that bacteria can be

resistance to one or more drugs. RpoB gene is one target of most effective drugs: rifampicin,

changes within this gene will make drugs is not effective. The aim of this research is to explore

the RpoB gene from the multidrug resistance Mtb isolate which is isolated from TB suspected

patient. Mtb isolated then observe both phenotype and genetic. As much as 30 sputum specimen

was collected, all of them is examine by microscopic and further culture and DNA Extraction for

PCR using specific primer. Among 30 specimens has been collected, all of them is AFB smears

positive. Drugs susceptibility testing result is vary, but all of them is Rifampicin resistance,

followed by resistance to one or more other drugs. Sequence analysis show that mutation has

found is nucleotide change and insertion.

Keywords : MDR, RpoB, Mutation, Sanglah

66

HYPOGLYCAEMIC EFFECTS OF ZYZYGUM POLYANTHUM LEAVES EXTRACT

ON ALLOXAN-INDUCED THROUGH DECREASE OF BLOOD GLUCOSE AND 8-

HYDROXY-2 DEOXYGUANOSINE (8-OHDG) IN HYPERGLYCEMIC WISTAR RATS

Sri Wahjuni 1; I Wyn Wita

2

1 Departement of Chemistry,Faculty of Mathematic and Natural Science Udayana University Campus Bukit

Jimbaran street 80361 Bali Indonesia 2 Clinical Internal Departement, Faculty of Medicine, Udayana University, PB. Sudirman Street Denpasar Bali

Indonesia

ABSTRACT

Hyperglyemia is a condition with an increase of blood glucose fasting levels (above 109 mg/dL)

and 2-hour blood glucose post prandial (above 158 mg/dL). Hyperglycemia increases reactive

oxygen species (ROS) through the process of enzymatic reactions. This reaction includes

oxidation and phosphorylation and ADPH-Oxidase reaction and through non-enzymatic process

by forming of Gluco oxidants and Glycation . Hyperglycemia is caused by abnormalities in

insulin secretion or action of insulin disorders. The state of hyperglycemia in diabetes lead to

increase formation of free radicals, antioxidants and decrease a number of events that eventually

occurs is called oxidative stress. Hyperglycemia can induce an increase in free radicals through

glucose auto-oxidation, the formation of Advanced Glycation End Products (AGES), and incre

ase of polyol pathway activity (sorbitol). The process outooxidation non hyperglycemia and

glycation reactions result in the release of electrons. The release of the electrons will trigger the

formati on of free radicals (RB) particulary superoxide radicals (O2), and hydrogen peroxide

(H2O2) and via Haber-Weis and Feton will form hydroxyl radicals (OH.). These materials are

known as oxygen free radicals ( RBO), which scan damage cell membranes (8-OHdG) and

glucose blood are known to diabetic Wistar Rats. Zyzygum polyanthum leaves extract rich in

phytol and phytol acetat is the same chlorofil. Extract zyzygum polyanthum has an ability to

prevent hiperglicemic disease. This is caused by the present of phytol and phytol acetat in the

zyzygum polyanthum leaves extract which has been phytol-chemistry tested and analyzed by

GC-MS such as phytol and phytol acetat substance.This research aimed to study the effect of

zyzygum polyanthum leaves extract in inhibiting stress oxidative in hyperglycemic. A

randomized pre-test and posttest control group design was performed on 40Wistar rats by

analyzing blood glucose,8-Hydroxy-2 Deoxiguanosin (8OHdG). Extraction of 3 kg zyzygum

polyanthum leaves ) with etanol gave 0,730 kg crude extract. Hyperglycemic on Wistar rats was

made by inducing hyperglycemic with alloxan 2 week. The experimental was performed on five

groups of animals that were Hyperglycemic: P0 for normal rats feed standar (control positip),P1

for normal rats alloxan induced with not intake zyzygum polyanthum (control negatip), P2 for

hyperglycemia rats alloxan induced with intake zyzygum polyanthum leaves extract 0.5 mg kg-1

body weight, P3 for hyperglycemia rats allozan induced with intake zyzygum polyanthum

leaves extract 2.0 mLkg-1 body weight; P4 for hyperglycemia rats alloxan induced with intake

zyzygum polyanthum leves extract 5.0 mLkg-1 body weight. P5 for hyperglycemia rats alloxan

induced with treatment glibenclamide 0,18 mg/day/200g body weight. The result showed that

intake of zyzygum polyanthum leaves extract in 5,0 mLkg-1

body given have decreased the

activity of blood glucose 65.91 %, decreased 8-OHdG 50.20%.

Keywords: Zyzygum polyanthum leaves extract extract; blood glucose; 8-Hydroxy-2

Deoxyguanosine ( 8-OHdG)

67

COMMITTEE

Advisors

Prof. Dr. dr. Putu Astawa, SpOT (K), M.Kes

Prof. Dr. dr. I Gede Putu Adiatmika, M.Kes

Executive Committee

Chairman : Dr. rer. nat. dr. Ni Nyoman Ayu Dewi, M.Si.

Representative chairman : dr. I Wayan Surudarma, M.Si

Secretary : dr. Agung Nova Mahendra, M.Sc

Treasurer : Nyoman Sri Handayani, S.Si

Event Division

Seminar :

Dr. dr. Desak Made Wihandani, M.Kes

dr. I Kadek Swastika, M.Kes

Ni Komang Tri Widianingsih, A.Md, A.K

I.A. Kade Ratna Sukmadewi, A.Md, A.K

Molecular Biology Workshop :

I.B.N. Putra Dwija, S.Si., M.Biotech.

Ni Ketut Nanik Astuti, S.Si

Ni Putu Senshi Septiasari, S.Si., M.Si

I Gd Wayan Topan Yogi Dyana, ST.

ELISA Workshop :

Dr. dr. I Nyoman Wande, SpPK

Amy Yelly Kusmawati, SKM, MP.

I Ketut Gede Adi Santika, A.Md, A.K

Poster :

dr. I G Kamasan Nyoman Arijana, M.Si.Med

I Made Angga Baskara, A.Md, A.K

Secretariat and Public Relation :

dr. Putu Gede Supadmanaba, S.Ked.

dr. IA. Dewi Wiryanthini, M.Biomed

I Made Indrawarma, S.Kom

Consumption :

Ni Wayan Sudarni

Ni Wayan Noni

Ni Wayan Niti

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Equipment :

dr. Wayan Gede Sutadarma, SpGK

I Dewa Ketut Alit Sumarsana, Amd.

I Made Arjana, Amd.

Gede Wiranatha, S.Si.

I Dewa Gede Wira Satya Utama, S.Kom

I Made Suardana

Documentation :

I Nyoman Agus Tinajaya

A.A. Ngurah Mayun S.T

Transportation :

Drs. I Ketut Kartika

I Wayan Adnyana Wijaya, S.P