2000

«51

Joint Protein Meeting Tokyo 200051st Forum for Protein Structure

12th Annual Meeting of the Protein Engineering Society of Japan7th Workshop on Principles of Protein Architecture

Program and Abstracts

UB/TIB Hannover 89121 321 827

Joint Protein Meeting (Tokyo 2000)

Program

June 7 (Wed)

Opening remark 12:55—13:00 in the Main Hall on the 1 st floor

Workshopi: Principles of protein architecture 13:00—18:30

W-01 Estimate of stabilization factors of proteins

Katsuhide Yutani (Osaka University)

W-02 Protein architecture revealed by molecular dissection: G-peptide composed of only 16 amino acids folds/unfolds as a

fashion of two-state phase transition

Shinya Honda (National Institute of Bioscience and Human-Technology)

W-03 Folding study from a point of view of a sequence space searching: Systematic circular permutation analysis and

topological mutation

Masahiro Iwakura (National Institute of Bioscience and Human-Technology)

W-04 Bacteriophage-encoded molecular chaperone and its role in the assembly of the virion

Fumio Arisaka (Tokyo Institute of Technology)

W-05 Crystal structure and function of a homing endonuclease encoded by the gene of a hyperthermophilic archaeon

Kenji Ichiyanagi, Yoshizumi Ishino, Mariko Ariyoshi, Kayoko Komori, Kosuke Morikawa (Biomolecular Engineering Research

Institute)

W-06 Proteasomes: Structure and biology

Keiji Tanaka (Tokyo Metropolitan Institute of Medical Science)

W-07 Dynamic aspect of protein structures revealed by single molecule detection

Yoshiharu Ishii, Tetsuichi Wazawa, Hiroaki Yokota, Takashi Funatsu, Yuji Goto, Toshio Yanagida (Japan Science and

Technology Corporation, Waseda University, Osaka University)

W-08 Structural changes of myosin motor domain during force generation

Kazuo Sutoh, Naoya Sasaki, Yoshikazu Suzuki, Reiko Ohkura (University of Tokyo)

W-09 Molecular mechanisms of self-assembly and polymorphic switching of the bacterial flagellum

Keiichi Namba (Japan Science and Technology Corporation, Matsushita Electric Industrial Co.)

W-10 Conformational dynamics of native protein

Nobuhiro Go (Kyoto University)

June 8 (Thu)

930 11 00 in the Main Hall on the 1 st floor

O-01 The origins of repetitiousness in proteome

Kiyotaka Shiba (Cancer Institute)

O-02 Molecular evolution of three-dimensional structure of vertebrate hemoglobins and their functions

Yukie Naoi, Khoon Tee Chong, Gentaro Miyazaki, Sam Yong Park, Hideki Morimoto (Osaka University, RIKEN)

O-03 De novo design of globular proteinsYasuhiro Isogai, Motonori Ota, Anna Ishii, Tetsuro Fujisawa, Ken Nishikawa (RIKEN, National Institute of Genetics,

Gakushuin University)

(—11:00 The posters from P2-01 to P2-92 should be put up in the Poster Session Room on the 3rd floor.)

11 00—14:00 Poster session (P2-01 — P 1 -92) in the Poster Session Room on the 3rd floor

Workshop2: S t ruc tu ra l and func t iona l studies on prote in molecules approach ing t o e luc idate physiological

phenomena and causes of diseases 14:00—19:00 in the Main Hall on the 1 st floor

W-11 X-ray structure and reaction mechanism of cytochrome c oxidase

Shinya Yoshikawa (Himeji Institute of Technology)

W-12 Solution structure and function of adaptor protein Grb2

Satoru Yuzawa, Masashi Yokochi, Hideki Hatanaka, Kenji Ogura, Mikio Kataoka, Kin-ichiro Miura, Valsan Mandiyan, Joseph

Schlessinger, Fuyuhiko Inagaki (Hokkaido University, Gakushuin University, Japan Science and Technology Corporation,

Tokyo Metropolitan Institute of Medical Science, Nara Institute of Science and Technology, New York University Medical

Center)

W-13 Molecular anatomy of transcription apparatus

Akira Ishihama, Nobuyuki Fujita, Tasuku Nomura, Akira Katayama, Olga N. Ozoline, Jeff Owens, Fred Colland, S. Ramesh

Wigneshweraraj, Sujatha Sitraman, A. Azam Talkuder, Hiroto Maeda, Shizuko Endo, Katsunori Yata, Dipankar Chatterji,

Kan Tanaka, Miki Jishage, Katsuhiko Murakami, Dipak Dasgupta (National Institute of Genetics, Institute of Cell

Biophysics, University of California, Institut Pasteur, Imperial College of Science, Technology and Medicine, Kagoshima

University, Indian Institute of Science, University of Tokyo, Saha Institute of Nuclear Physics)

W-14 Structure and function of the ribosomal proteins at ribosome functional domain

Isao Tanaka (Hokkaido University)

W-15 Ribosomal acidic protein complex as a part of the " translational engine"

Toshio Uchiumi, Sachiko Honma, Takaomi Nomura, Akira Hachimori (Shinshu University)

W-16 Molecular structures controlling morphological transitions of viruses

Hideki Hatanaka, Elaine Hendry, Elizabeth Fry, Michael Smyth, John Tate, Oleg Iourin, Zihe Rao, Karl Harlos,

Alan Kingsman, Glyn Stanway, Juhana Santti, Marita Maaronen, Timo Hyypia, David Stuart (University of Oxford,

Tokyo Metropolitan Institute of Medical Science, University of Essex, University of Turku, University of Helsinki)

W-17 Prion protein and prion diseases

Motohiro Horiuchi (Obihiro University of Agriculture and Veterinary Medicine)

W-18 Mechanism of conversion of xanthine dehydrogenase to xanthine oxidase and role of postrischemic reperfusion injury

Takeshi Nishino, Ken Okamoto, Toshio Iwasaki, Hiroyuki Hori, Tomoko Nishino, Cristofer Enroth, Bryan T. Eger, Emil F. Pai

(Nippon Medical School, Yokohama Crty University, The University of Toronto)

W-19 How can structure^unction relationship of calpain elucidate molecular mechanisms of muscular dystrophies ?

Hiroyuki Sorimachi, Yasuko Ono, Shoji Hata, Eiichi Kimura, Kazuhiko Nakagawa, Yukiko Katsui, Kazumi Kakinuma,

Kazuhiko Tagawa, Hajime Masumoto, Akihiro Irie, Koichi Suzuki (University of Tokyo, Japan Science and Technology,

National Institute of Neuroscience, RIKEN, Tokyo Metropolitan Institute of Gerontology)

( ~ 19:30 The posters from P2-01 to P2-92 should be removed.)

June 9 (Fri)

9:30-11:00 in the Main Hall on the 1 st floor

0 -04 Thermostabilization of a psychrophilic subtilisin by directed evolution

Kentaro Miyazaki (National Institute of Bioscience and Human-Technology)

O-05 Structural basis for the thermostability of DNA binding protein HU from Bacillus steamthermophilus

Shunsuke Kawamura, Makoto Kimura (Kyushu University)

0 -06 Crystal structures of 3-isopropylmalate dehydrogenases with mutations at the C-temninus: Crystallographic analyses of

structure-stability relationships

Zeiry Nurachman, Satoshi Akanuma, Takao Sato, Nobuo Tanaka, Tairo Oshima (Tokyo Institute of Technology, Tokyo

University of Pharmacy and Life Science)

O-07 Functional alteration of an antibody with the desired specificity and the crystallographic analysis of engineered

antibody-target antigen complex

Yoshiyuki Nishimiya, Kouhei Tsumoto, Mitsunori Shiroishi, Hidemasa Kondo, Izumi Kumagai (Tohoku University, Hokkaido

National Industrial Research Institute)

(—11:00 The posters from P3-01 to P3-90 should be put up in the Poster Session Room at the 3rd floor.)

11:00—14:00 Poster session ( P 3 - 0 1 - P 3 - 9 0 ) in the Poster Session Room on the 3rd floor

14:00—17:30 in the Main Hall on the 1 st floor

O-08 Mode coupling in protein molecules

Osamu Miyashita, Kei Moritsugu, Akinori Kidera (Kyoto University)

O-09 Volume—A new dimension for structural biology

Kazuyuki Akasaka, Kyoko Inoue, Ryo Kitahara, Hua Li, Hiroaki Yamada (Kobe University)

O-10 Folding of bovine /3 -lactoglobulin analyzed by NMR

Masaru Hoshino, Vincent Forge, Kazuo Kuwata, Carl A. Batt, Yuji Goto (Osaka University, Gifu University, Cornell

University)

O-11 A structural study of disulfide regeneration-intermediates of hen lysozyme by NMR

Shirr-ichi Segawa, Atsushi Yokota, Takeshi Tominaga, Yoshiaki Tanisaka, Nobuyuki Takagi, Yasuo Noda, Hideki Tachibana

(Kwansei Gakuin University, Kobe University)

0 -12 Canine milk lysozyme: As a model protein for studying protein folding

Takumi Koshiba, Yoshihiro Kobashigawa, Kunihiro Kuwajima, Katsutoshi Nitta (Hokkaido University, University of Tokyo)

0 -13 Direct observation of the chain condensation process during cytochrome c folding using time-resolved circular dichroism

and small angle X-ray scattering techniques

Shuji Akiyama, Satoshi Takahashi, Koichiro Ishimori, Isao Morishima, Yukihiro Nishikawa, Tetsuro Fujisawa (Kyoto

University, Japan Science and Technology Corporation, RIKEN)

0 -14 Useful concepts and key technological items induced from successful refolding of several globular proteins

Tomoya Sakai, Daisuke Nohara, Eiji Kurimoto (Nagoya City University)

( ~ 18:00 The posters from P3-01 to P3-90 should be removed.)

•\ 8;00 2ftO0 Party in the Small Hall on the 3rd floor

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June KXSat)

9:00—12:30 i n the Main Hall on the 1 st floor

0-15 Water structural changes involved in the activation process of photoactive yellow protein

Hideki Kandori, Tetsuya Iwata, Johnny Hendriks, Akio Maeda, Klaas J. Hellingwerf (Kyoto University, University of

Amsterdam)

0-16 Crystal structure of ADP-dependent glucokinase from a hyperthermophilic archaeon Thermococcus litoralis

Sohei Ito, Shinya Fushinobu, Issei Yoshioka, Shinji Koga, Hiroshi Matsuzawa, Takayoshi Wakagi (University of Tokyo,

Asahi Chemical Industry, Aomori University)

O-17 Crystal structure and dimerization of a soluble form of B7-1

Shinji Ikemizu, E. Yvonne Jones, David L Stuart, Simon J. Davis (University of Oxford)

0-18 Crystal structure of nucleotide excision repair protein, UvrB

Noriko Nakagawa, Mitsuaki Sugahara, Ryoji Masui, Ryuichi Kato, Keiichi Fukuyama, Seiki Kuramitsu (Osaka University,

RIKEN)

0-19 Fourier—transform infrared studies on interaction of parvalbumin with metal ions

Masayuki Nara, Masaru Tanokura, Mitsuo Tasumi (Tokyo Medical and Dental University, University of Tokyo, Sartama

University)

O-20 Noncooperative nucleotide binding and the ATP-induced cooperative transition of the chaperonin GroEL

Tomonao Inobe, Tadashi Makio, Etsuko Takasu-Ishikawa, Tomoki P. Terada, Munehito Arai, Kazuki Ito, Masaharu Nakao,

Hiroshi Kihara, Yoshiyuki Amemiya, Kunihiro Kuwajima (University of Tokyo, University of Tsukuba, Kansai Medical

University)

0-21 Structural stability of oligomeric chaperonin GroES and formation of amyloid fibril

Takashi Higurashi, Kaoru Ichimura, Yuzuai Hiragi, Tomohiro Mizobata, Jun Nagai, Yasushi Kawata (Tottori University,

Dokkyo University School of Medicine, Kyoto University)

Symposium : Proteome 13:30—16:30 in the Main Hall on the 1 st floor

Organizer Yasutsugu Shimonishi, Akira Tsugita

S-1 Peptide-mass fingerprinting of glycoproteins

Yoshinori Satomi, Toshrfumi Takao, Masami Matsubae, Yasutsugu Shimonishi (Osaka University)

S-2 Development of new target plate for matrix-assisted laser desorption ionization (MALDI)

Hiroyuki Fukuda, Toshrfumi Takao, Yasutsugu Shimonishi (PE Biosystems Japan, Osaka University)

S-3 Novel method for the sequence analysis of protein

Mitsuo Takayama, Akira Tsugita (Toho University, Proteomics Research Laboratory)

S-4 A novel approach to the peptide sequence analysis with fluorescent modification using posirsource decay fragment of

MALDHMS

Tohru Yamagaki, Masatoshi Nakagawa, Hiroshi Nakanishi (University of Tokyo, National Institute of Bioscience and

Human-Technology, Science University of Tokyo)

S-5 Peptidome : Comprehensive fact-database for endogenous peptides

Naoto Minamino (National Cardiovascular Center Research Institute)

S-6 Proteome analysis of Cyanobacteria, Synechocystis sp. strain PCC6803 and Anabaenasp. strain PCC7120

Takashi Sazuka, Jun Yamazaki, Takakazu Kaneko, Satoshi Tabata, Osamu Ohara (Kazusa DNA Research Institute, National

Institute of Technology and Evaluation)

S-7 Proteomic analysis of tumor suppressor related proteins in brain

Norie Araki, Hideyuki Saya, Hiroshi Tokuo, Shunji Yunoue, Tadaomi Morimasa, Kenji Miyazaki, Akira Tsugita (Kumamoto

University, Okayama University, Proteomics Research Laboratory)

S-8 Analysis of post-translational modification in protein

Akira Tsugita, Kenji Miyazaki, Takuji Nabetani (Proteomics Research Laboratory)

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Poster session

Structural information analysis of proteins

P2-01 Construction of database for electrostatic-surface of Functional site (eF-site)

K. Kinoshita, J. Furui, H. Nakamura (RIKEN, Osaka University)

P2-02 Protein function prediction by comparing molecular surfaces based on geometric hashing

K. Matsuura, N. Shoji, T. Ohkawa, H. Nakamura (Osaka University)

P2-03 A method of classifying active sites of antibodies based on region-division-matching of surface Characteristicpictures

K. Ikeda, T. Tanaka, T. Ohkawa, H. Nakamura (Osaka University)

P2-04 Development of domain identification method

M. Shionyu, M. Go (Nagoya University)

P2-05 Domain linker prediction by neural network

S. Miyazaki, Y. Kuroda, S. Yokoyama (University of Tokyo, RIKEN)

P2-06 Analysis of structural motifs in proteins

J. An, H. Wako, A. Sarai (RIKEN, Waseda University)

P2-07 The comparison method COSMAT of protein structures based on rough information of secondary structure

T. Nakagawa, N. Shoji, T. Ohkawa, H. Nakamura (Osaka University)

P2-08 Protein structure comparison by mean field approximation

R. Koike, A. Kidera (Kyoto University)

P2-09 A method of local structural alignment which can treat circular permutation

T. Hiroike, H. Toh (Biomolecular Engineering Research Institute)

P2-10 Sequence analysis of Holliday junction resolvase Hjc derived from archaebacteria

H. Toh, H. Daiyasu, T. Hiroike, K. Komori, Y. Ishino (Biomolecular Engineering Research Institute)

P2-11 Multiple structure matching for proteins based on 3D positions of secondary structure elements

A. Yamaguchi, K. Tomii, H. Toh (Biomolecular Engineering Research Institute)

P2-12 Variety and common aspects of phosphate binding site in proteins

K. Kinoshita, A. Kidera, N. Go (RIKEN, Osaka University, Kyoto University)

P2-13 Fold recognition of sequences in the HUNT full length cDNA database

H.T. Yudate, M. Suwa, Y. Masuho, K. Nagai (Helix Research Institute)

P2-14 Genome analysis: Assigning protein coding regions to 3D structures

M. Suwa, H.T. Yudate, A.A. Salamov, C.A. Orengo, M.B. Swindells (Helix Research Institute, The Sanger

Centre, University College London, Inpharmatica Ltd.)

P2-15 Different module organization and substrate specificity in 4/7 superfamily of glycoside hydrolases having TIM barrel

structure

Y. Sugiura, M. Shionyu, M. Go (Nagoya University)

P2-16 Classification of metaHiganding module

K. Yura, K. Iida, N. Matsui, M. Go (Nagoya University)

P2-17 Structural basis of presequence recognition by the mitochondrial protein import receptor Tom20

T. Muto, T. Shodai, Y. Abe, T. Endo, D. Kohda (Biomolecular Engineering Research Institute, Nagoya University)

P2-18 The development of signal peptide discrimination system

M. Gomi, S. Mitaku (Tokyo University of Agriculture and Technology)

P2-19 Functional classification of 7 transmembrane helix receptors

M. Suwa, H.T. Yudate, Y. Masuho, S. Mitaku (Helix Research Institute, Tokyo University of Agriculture and

Technology)

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P2-20 Graphic tool for the prediction of ft -sheet type membrane proteins

T. Hayashibe, S. Mitaku (Tokyo University of Agriculture and Technology)

P2-21 Secondary structure prediction and folding of protein—Structure prediction of ferredoxin

Y. Kobayashi, N. Saito (Soka University, Waseda University)

P2-22

P2-23

P2-24

P2-25 Development of protein-ligand interaction database

H. Kubodera, H. Ueno, M. Kamizono, M. Okamoto, J. An, S. Selvaraj, P. Prabakaran, A. Sarai (Mitsubishi-Tokyo

Pharmaceuticals, RIKEN)

P2-26 Full-atom molecular orbital study of RNase T1

K. Ohno, N. Kamiya, N. Asakawa, Y. Inoue, M. Sakurai (Tokyo Institute of Technology)

P2-27 SCF-LMO calculations for the elastic properties of alpha helix

F. Pichierri, A. Sarai (RIKEN)

P2-28 Forcefield dependency of the peptide structures 2

M. Kuroda, S. Ono, J. Higo, H. Nakamura (Biomolecular Engineering Research Institute, Tanabe Seiyaku, Osaka

University)

P2-29 Ab initio molecular orbital calculations of Ramachandran-type single amino acid potentials in water

M. Iwaoka, M. Okada, S. Tomoda (University of Tokyo)

P2-30 New homology modeling method and its evaluation

A. Hijikata, A. Yamaguchi, M. Go (Nagoya University)

P2-31 Homology modeling of human leptin/leptin receptor complex

T. Hiroike, J. Higo, H. Jingami, H. Toh (Biomolecular Engineering Research Institute)

P2-32 Molecular evolution of gene products of human long cDNAs

R. Kikuno, T. Nagase, 0. Ohara (Kazusa DNA Research Institute)

P2-33 Mathematical analysis of protein evolvability

H. Suzuki (ATR Human Information Processing Research Laboratories)

P2-34 Origin of protein module structures form the view of poly-tRNA hypothesis

K. Ohnishi, H. Shutou, S. Hokari (Niigata University)

Protein folding

P2-35 Multistep denaturation mechanism of outer surface protein A (OspA)

T. Nakagawa, H. Shimizu, A. Tamura, K. Akasaka, X. Huang, K. Link, A. Koide, S. Koide (Kobe University, University

of Rochester Medical Center)

P2-36 Incompletely denatured structure of a -lactalbumin at alkaline pH

K. Imai, H. Sakai, M. Sonoyama, S. Mitaku (Tokyo University of Agriculture and Technology)

P2-37 Effects of topological mutation on the native state and folding of dihydrofolate reductase

K. Maki, M. Iwakura (National Institute of Bioscience and Human-Technology)

P2-38 Further analysis of the fluorescence equilibrium unfolding mechanism of E coli 3-isopropylmalate dehydrogenase

using tryptophan mutants

C. Motono, K. Isshiki, T. Oshima, A. Yamagishi (Tokyo University of Pharmacy and Life Science)

P2-39 Alkaline-denatured structure of pepsin

T. Konno, Y. Kamatari, N. Tanaka, C. Dobson, K. Nagayama (National Institute for Physiological Sciences, Oxford

University, Kyoto Institute of Technology)

P2-40 Pressure induced dissociation of aggregated (S-S) deficient lysozyme

T.N. Niraula, H. Li, H. Yamada, T. Konno, H. Tachibana, K. Akasaka (Kobe University)

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P2-41 Mechanical unfolding of a single actin-binding protein ABP-280

S. Furuike, T. Ito, M. Yamazaki (Shizuoka University, Kyoto University)

P2-42 Protein unfolding studied by temperature-jump NMR

M. Kawakami, K. Akasaka (Kobe University)

P2-43 Pressure unfolding of a molten globule. A high pressure NMR study of all-Ala a -lactalbumin

M.W. Lassalle, H. Li, H. Yamada, K. Akasaka (Kobe University)

P2-44 The in vivo folding and in vitro refolding of green fluorescent protein

H. Fukuda, M. Arai, K. Kuwajima (University of Tokyo)

P2-45 Analysis of a folding reaction of Of-lactalbumin by stopped-flow solution X-ray scattering

M. Arai, K. Ito, T. Inobe, K. Maki, M. Nakao, H. Kihara, Y. Amemiya, K. Kuwajima (University of Tokyo, University of

Tsukuba, Kansai Medical University)

P2-46 Refolding of subtilisin by a protease inhibitor which is homologous to the propeptide of subtilisin

H. Yanai, K. Miura, S. Kojima (Gakushuin University)

P2-47 Elucidation of the process of protein folding using tandem apomyoglobin

M. Kondo, S. Akiyama, S. Takahashi, Y. Harada, K. Ishimori, I. Morishima (Kyoto University, Japan Science and

Technology Corporation, Shimadzu Corp.)

P2-48 Analysis of the folding intermediate of reduced mutant hen lysozyme where Trp62 is mutated to Gly in vitro

T. Ohkuri, T. Ueda, T. Imoto (Kyushu University)

P2-49 Characterization of the refolding and reassembly of Escherichia co/i outer membrane protein OmpF

Y. Watanabe, Y. Inoko (National Food Research Institute, Osaka University)

P2-50 Movement of the position of the transition state on the folding pathway of a small protein, POIA1

S. Tanaka, 0. Kambara, A. Tamura, K. Akasaka, Y. Harada, S. Kojima, K. Miura (Kobe University, Shimadzu Corp.,

Gakushuin University)

P2-51 Dependence of the hydration structure of proteins on their conformation

K. Soda, Y. Seki, S. Kidokoro (Nagaoka University of Technology)

P2-52 Helix-forming tendencies of oligopeptides by a generalized-ensemble algorithm

A. Mitsutake, Y. Okamoto (Institute for Molecular Science, The Graduate University for Advanced Studies)

P2-53 The role of folding intermediates in a —* ft transition—A lattice model study

G. Chikenji, M. Kikuchi (Osaka University)

P2-54 Solvent effect on protein thermal unfolding studied with MD simulations

T. Takahashi, K. Nagayama (National Institute for Physiological Sciences)

P2-55 Imprinted folding habit with nascent proteins

T. Sakai (National Institute of Bioscience and Human-Technology)

P2-56 Analysis of the differences among the folding mechanisms of fatty-acid binding protein, cellular retinoic binding

protein I and II based on the predicted contact maps using the statistics of average distances between residues

T. Ichimaru, T. Kikuchi (Kurashiki University of Science and the Arts)

P2-57

P2-58 Capacity of the folding cavity formed by molecular chaperons GroEL and GroES

T. Miyazaki, M. Kawagoe, T. Mizobata, J. Nagai, Y. Kawata (Tottori University)

P2-59 Analysis of the solution X-ray scattering profile of chaperonin GroEL

Y. Seki, Y. Hiragi, K. Ichimaru, K. Soda (Nagaoka University of Technology, Kyoto University, Dokkyo University

School of Medicine)

P2-60 Role of N- and C-terminal ft -strands in the chaperonin GroES structural stability

C. Matsumoto, T. Higurashi, T. Mizobata, J. Nagai, Y. Kawata (Tottori University)

P2-61 Molecular mechanism of chaperonin GroEL in the presence of the nucleotides

T. Makio, E. Takasu, K. Kuwajima (University of Tokyo)

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Protein dynamics

P2-62 Structure refinement of the scTCR by NMR and JAM model

A. Kitao, B. Hare, G. Wagner (Kyoto University, Harvard Medical School)

P2-63 Normal mode refinement Refinement of protein dynamic structure against X-ray data at atomic resolution

Y. Jochi, M. Nakasako, A. Kidera (Kyoto University, Japan Science and Technology Corporation & University of

Tokyo)

P2-64 Normal mode analysis of a gp120(HIV-1)-CD4 complex

C. Ohuchi, Y. Kurihara, M. Takeda-Shitaka, K. Kamiya, H. Umeyama (Kitasato University)

P2-65 Molecular dynamics simulations of energy transfer among normal modes in proteins

S. Endo, H. Wako (Kitasato University, Waseda University)

P2-66 Vibrational energy transfer in a protein molecule

K. Moritsugu, 0. Miyashita, A. Kidera (Kyoto University)

P2-67 Chemical bonding effects in electron scattering by molecules

H. Yamashita, A. Kidera (Kyoto University)

P2-68 Reorganization energy of protein electron transfer reaction

O. Miyashita, N. Go (Kyoto University)

P2-69 Quantum chemical study of pKa shifts in photoactive yellow protein

M. Sakurai, M. Yoda, Y. Inoue (Tokyo Institute of Technology)

P2-70 Molecular dynamics simulation on the pB intermediate of photoactive yellow protein

M. Shiozawa, M. Yoda, N. Kamiya, N. Asakawa, Y. Inoue, M. Sakurai (Tokyo Institute of Technology)

P2-71 Opening and closing anti-correlation motions of two domains of a-helix groups in the peptide-binding cleft of

human HLA-A2 class I MHC proteins

Y. Kurihara, M. Takeda-Shitaka, K. Tsuda, H. Nojima, S. Yoneda, K. Kamiya, H. Umeyama (Kitasato University)

P2-72 Application of Replica-exchange Monte Carlo method for isobaric-isothermal ensemble

T. Nishikawa, Y. Sugita, M. Mikami, Y. Okamoto (Institute for Molecular Science, National Institute of Materials and

Chemical Research, The Graduate University for Advanced Studies)

P2-73 Pressure effect on the conformational fluctuation of apomyoglobin in the native state

N. Tanaka, C. Ikeda, K. Kanaori, K. Hiraga, T. Konno (Kyoto Institute of Technology, National Institute for

Physiological Sciences)

P2-74 Spectral fluctuation of fluorophores conjugated to a protein observed by single-molecule fluorescence

spectroscopy

T. Wazawa, Y. Ishii, T. Yanagida (Japan Science and Technology Corporation, Osaka University)

Protein stability

P2-75 Energy landscape of peptides in water

J. Higo, N. Ito, S. Ono, O.V. Galzitskaya, M. Kuroda, H. Nakamura (Biomolecular Engineering Research Institute,

Tanabe Seiyaku, Osaka University)

P2-76 Free energy landscape of proline cis-trans isomerization (2)

S. Ono, J. Higo, H. Nakamura (Biomolecular Engineering Research Institute, Osaka University)

P2-77 A new method of free energy calculation by replica-exchange method

Y. Sugita, A. Kitao, Y. Okamoto (Institute for Molecular Science, Kyoto University, The Graduate University for

Advanced Studies)

P2-78 Thermodynamic database for protein-nucleic acid interactions

P. Prabakaran, J. An, M.M. Gromiha, S. Selvaraj, H. Uedaira, H. Kono, A. Sarai (RIKEN, University of Pennsylvania)

P2-79 Thermodynamic database for proteins and mutants: ProTherm Version 2.0

H. Uedaira, M.M. Gromiha, J. An, H. Kono, M. Oobatake, P. Prabakaran, S. Selvaraj, A. Sarai (RIKEN, University of

XXIV

Pennsylvania, Meijo University)

P2-80 Relationship between amino acid properties and protein stability upon surface mutations

M.M. Gromiha, M. Oobatake, H. Kono, H. Uedaira, A. Sarai (RIKEN, Meijo University, University of Pennsylvania)

P2-81 Thermodynamic characteristics of stalk region of ncd motor protein

H. Morii, M. Ito, T. Shimizu, M. Tanokura (National Institute of Bioscience and Human-Technology, University of

Tokyo)

P2-82 Effects of mutations in the hydrophobic core of Streptomyces subtilisin inhibitor on its inhibitory activity and

stability

M. Shimizu, K. Miura, S. Kojima (Gakushuin University)

P2-83 Are the parameters of various stabilization factors calculated from mutant human lysozymes compatible to other

proteins ?

J. Funahashi, K. Takano, K. Yutani (Osaka University)

P2-84 Thermodynamic analysis of heat denaturation for pyrrolidone carboxyl peptidase C142/188S from

hyperthermophile

K. Ogasawara, J.K. Kaushik, S. Kidokoro, K. Yutani (Osaka University, Nagaoka University of Technology)

P2-85 Contribution of salt bridges to the conformational stability of a protein

K. Takano, K. Tsuchimori, Y. Yamagata, K. Yutani (Osaka University)

P2-86 The effect of a mutation of glycine in a loop of Escherichia coli 3-isopropylmalate dehydrogenase which was

found by experimental evolution

Y. Miyajima, M. Tamakoshi, A. Yamagishi, T. Oshima (Tokyo University of Pharmacy and Life Science)

P2-87 Energetic contribution of Thr29 in the hydrophobic core to thermodynamic stability of goat a -lactalbumin

mutants

K. Horii, M. Saito, T. Yoda, K. Tsumoto, M. Matsushima, K. Kuwajima, I. Kumagai (Tohoku University, Hirosaki

University, University of Tokyo, Rational Drug Design Laboratory)

P2-88 Enhancement of the stability of subtilisin E by introduction of a disulfide bond

H. Takagi, K. Hirai, M. Wada, S. Nakamori (Fukui Prefectural University)

P2-89 Cooperative effect of mutation on thermostability of lactate oxidase

H. Minagawa, H. Kaneko, J. Shimada (NEC Corp.)

P2-90 Directed evolution of thermostable kanamycin-resistance gene in an extreme thermophile, Thermus thermophilus

J. Hoseki, T. Yano, Y. Koyama, S. Kuramitsu, H. Kagamiyama (Osaka University, Osaka Medical College, National

Institute of Bioscience and Human-Technology)

P2-91 Characterization of cold-denatured state of ft -lactoglobulin

H. Katou, M. Hoshino, Y. Goto (Osaka University)

P2-92 Systematic stabilization of mesophilic cytochrome c based on the three dimensional structure of a thermophilic

counterpart

J. Hasegawa, S. Uchiyama, Y. Tanimoto, M. Mizutani, Y. Kobayashi, Y. Sambongi, Y. Igarashi (University of Tokyo,

Osaka University)

Expression and preparation of proteins

P3-01 Expression of an erythropoietin receptor (the extracellular domain) in Escherichia coli and characterization of the

expressed protein

T. Koshiba, H. Yamamoto, K. Nitta, R. Kuroki (Hokkaido University, Kirin Brewery Co.)

P3-O2

P3-O3 Expression and characterization of human chemokine receptor, CXCR1, in baculovirus-infected insect cells

Y. Maeda, R. Kuroki, H. Michel, H. Reilaender (Kirin Brewery Co., Max-Planck-Institute for Biophysics)

XXV

P3-04 Construction of refolding system of proteins with immunoglobulin-fold prepared from inclusion bodies

K. Hata, T. Kitamura, K. Tsumoto, S. Misawa, I. Kumagai (Tohoku University, Japan Energy Corp.)

Protein engineering

P3-05 Design of mini-protein by removing a module from barnase and its conformational measurements

K. Takahashi, T. Noguti, H. Hojo, K. Yamauchi, M. Kinoshita, S. Aimoto, T. Ohkubo, M. Go (Nagoya University,

Fukuoka Institute of Technology, Tokai University, Osaka City University, Osaka University)

P3-06 Design of linker to separate domains of bi-fijnctional chimeric protein

R. Arai, H. Ueda, A. Kitayama, N. Kamiya, T. Nagamune (University of Tokyo)

P3-07 Design of a new myoglobin by introducing a metal binding loop

A. Nishiyama, M. Ihara, K. Wakasugi, S. Takahashi, K. Ishimori, I. Morishima (Kyoto University)

P3-08 Generation of globin-like function with electron transport protein by the alteration of a heme axial ligand

N. Kamiya, H. Morii, T. Noguchi, T. Nagamune (University of Tokyo, National Institute of Bioscience and

Human-Technology, RIKEN)

P3-09 Structural and functional properties of designed globins

A. Ishii, M. Ishida, Y. Isogai, T. Fujisawa, M. Ota, T. Iizuka, K. Nishikawa (Gakushuin University, Hosei University,

RIKEN, National Institute of Genetics)

P3-10 The axial cytochrome 4 mutants having an unusual high O2 affinities : Novel stabilization mechanism for O2-heme

iron complex

M. Ihara, M. Shintaku, S. Takahashi, K. Ishimori, I. Morishima (Kyoto University)

P3-11 Fragment-condensation of peptides using subtilisin which is chemically modified at the catalytic residue

H. Yuminaga, S. Ogawa, T. Nakazawa (Nara Women's University)

P3-12 Mapping of cleavage and duplication sites of tryptophan synthase a subunit

K. Hiraga, A. Yamagishi, T. Oshima (Tokyo University of Pharmacy and Life Science)

P3-13 Analysis of cold-adapted mutants of 3-isopropylmalate dehydrogenase from an extreme thermophile Thermus

thermophilus

M. Yasugi, T. Suzuki, M. Amino, E. Munekata, A. Yamagishi, T. Oshima (University of Tsukuba, Tokyo University of

Pharmacy and Life Science)

P3-14 Construction and characterization of linked-dimeric water soluble PQQ glucose dehydrogenase

K. Sode, Y. Kohiki, M. Shirahane, H. Yoshida (Tokyo University of Agriculture and Technology)

P3-15 Making specificity of protease inhibitor by random mutation

S. Morimoto, N. Hamamatsu, Y. Shibanaka (Novartis Pharma K.K.)

P3-16 Stabilization of human ribonuclease 1 dimer by increased inter-subunit interactions

S. Ishikami, H. Tada, J. Futami, A. Takidani, T. Hirata, M. Kosaka, M. Seno, H. Yamada (Okayama University)

P3-17 Construction of AAB-type helical bundle by hydrophobic core engineering

A. Kashiwada, H. Hiroaki, D. Kohda, T. Tanaka (Biomolecular Engineering Research Institute)

P3-18

Analysis of interaction

P3-19 Mechanism of conformational and functional change in a ,-acid glycoprotein by interaction with membranes

K. Nishi, T. Maruyama, M. Otagiri (Kumamoto University)

P3-20 Alteration of antigen recognition through affinity and avidity changes during affinity maturation

M. Oda, T. Azuma (Science University of Tokyo)

P3-21 The alteration of affinity toward glucose by introducing site directed mutations into loop regions of water soluble

PQQ glucose dehydrogenase

K. Sode, M. Fujikawa, A. Morimoto, S. Igarashi, H. Yoshida (Tokyo University of Agriculture and Technology)

YYVI

P3-22 Side-chain orientation of binding surface of ribonuclease inhibitor

N. Takahashi, Y. Komeiji, T. Ohyanagi, N. Matsushima (Hokkaido University of Education, National Institute for

Advanced Interdisciplinary Research, Sapporo Medical University)

P3-23 A structural analysis of sugar binding sites in proteins

K. Sadanami, K. Kinoshita, A. Kidera, N. Go (Kyoto University, Osaka University)

P3-24 Identification of the binding domain of apo-calmodulin with IQ-motif peptide

M. Mori, T. Konno, K. Imoto, K. Nagayama (National Institute for Physiological Sciences)

P3-25 Investigation of the structural basis for the substrate specificity of ribonuclease isolated from bitter gourd seeds

T. Numata, M. Kimura, N. Yamasaki, A. Suzuki, I. Tanaka (Kyushu University, Hokkaido University)

P3-26 Structural modification of hevein domains and the effect on the affinity for chitin

M. Muraki, H. Morii, K. Harata (National Institute of Bioscience and Human-Technology)

P3-27 Synergism of polar and apolar interactions in the carbohydrate-ligand recognition of human lysozyme

M. Muraki, K. Harata, N. Sugita, K. Sato (National Institute of Bioscience and Human-Technology, Kanagawa

University)

P3-28 An extension of hemoglobin allosteric model under a wide range of allosteric constraints

K. Imai, S.I. Park, A. Tsuneshige, T. Yonetani (Osaka University, University of Pennsylvania Medical Center)

P3-29 Interaction between rubredoxin and desulfoferrodoxin

T. Nakanishi, H. Inoue, M. Kitamura (Osaka City Universuty)

P3-30 Study of medical polymer-protein interaction by infrared spectroscopy

H. Maeda, S. Suzuki, Y. Yokoyama, R. Ishiguro, K. Kameyama, K. Hiramatsu (Gifu University)

P3-31 N-terminal myristoylation-dependent protein-protein interaction

N. Hayashi, Y. Ito, A. Takasaki, T. Shibata, Y. Izumi, N. Matsushima, K. Titani (Fujita Health University, RIKEN,

Yamagata University, Sapporo Medical University)

P3-32 Investigation of the interaction between thrombopoietin and neutralizing monoclonal antibody TNI by isothermal

titration calorimetry

T. Tamada, Y. Matsukura, R. Kuroki (Kirin Brewery Co.)

P3-33 Analysis of molecular interactions between NS3 protease of HCV and NS3 aptamers using BIACORE

H. Fauzi, J. Hwang, K. Fukuda, S. Sekiya, I. Kusakabe, N. Kakiuchi, S. Nishikawa (National Institute of Bioscience and

Human-Technology, University of Tsukuba)

P3-34 Subunit assembly and association equilibrium of a molecular chaperone-like protein gp57A of bacteriophage T4

N. Iwabuchi, F. Arisaka (Tokyo Institute of Technology)

Nucleic acid binding proteins

P3-35 DNA binding of a new basic leucine-zipper protein with a novel DNA contacting domain

T. Morii, S. Sato, K. Makino (Kyoto University)

P3-36 Thermodynamic stability of point mutants at core region of c-Myb DNA-binding domain

H. Morii, M. Saito, H. Kono, K. Ogata, M. Oda, H. Uedaira, A. Sarai (National Institute of Bioscience and Human-

Technology, Hirosaki University, University of Pennsylvania, Kanagawa Academy of Science and Technology,

Science University of Tokyo, RIKEN)

P3-37 Anatomy of specific interactions between Cro repressor and operator DNA

M. Oobatake, Y. Wang, H. Kono, A. Sarai (Meijo University, RIKEN, University of Pennsylvania)

P3-38 Free-energy landscape of base-amino acid interactions: Specificity in the protein-DNA recognition

A. Sarai, H. Kono, M. Gromiha, K. Sayano, F. Pichierri, M. Aida (RIKEN, University of Pennsylvania, Electrotechnical

Laboratory, Hiroshima University)

P3-39 Structure-specificity relationship in protein-DNA recognition studied by structure-based potentials

S. Selvaraj, P. Prabakaran, H. Kono, A. Sarai (RIKEN, University of Pennsylvania)

xxvo

P3-40 Nuclear localization signal of transcription factor Sp1: Zinc finger

J. Kuwahara, M. Azumano, Y. Watanabe, K. Itoh, M. Niwa (Tokushima University)

P3-41 NMR structural study of the transcriptional control ATF-2

A. Nagadoi, S. Hanaoka, H. Okamura, T. Maekawa, S. Ishii, Y. Nishimura (Yokohama City University, RIKEN)

P3-42 Molecular dynamics study of mononucleotide binding proteins

M. Ikeguchi, S. Nakamura, K. Shimizu (University of Tokyo)

Membrane proteins

P3-43 Mechanism of proton transport in bacteriorhodopsin—The importance of the water molecule

K. Murata, Y. Fujii, M. Hata, T. Hoshino, M. Tsuda (Chiba University)

P3-44 Cotranslational protein topogenesis of multispan membrane proteins on the endoplasmic reticulum membrane

A. Kitamura, N. Hamasaki, K. Mihara, M. Sakaguchi (Kyushu University)

P3-45 Induction of turn-structure is critical for translocation of N-domain of type I signal-anchor protein across

membrane

Y. Kida, K. Mihara, M. Sakaguchi (Kyushu University)

P3-46 Stability of membrane/ Oi -lactalbumin intermediates binary system

H. Sakai, K. Imai, M. Sonoyama, S. Mitaku (Tokyo University of Agriculture and Technology)

P3-47 Light-induced denaturation of bacteriorhodopsin during the thermal denaturation processes

Y. Yokoyama, M. Sonoyama, S. Mitaku (Tokyo University of Agriculture and Technology)

P3-48 Interaction between Influenza-hemagglutinin fusion peptide and SDS micelle

K. Chiba-Kamoshida, H. Nakanishi (National Institute of Bioscience and Human-Technology)

Molecular assembly of proteins

P3-49 Effect of the terminal extension with glycine residues on the fibrous association of an amphipathic helix-forming

polypeptide

T. Takei, A. Okonogi, K. Tateno, K. Yazaki, S. Kojima, K. Miura (Gakushuin University, Tokyo Metropolitan Institute

of Medical Science)

P3-50 Atomic structure of the F41 fragment of flagellin: A clue to the switching mechanism

F.A. Samatey, K. Imada, S. Nagashima, T. Kumasaka, M. Yamamoto, K. Namba (Japan Science and Technology

Corporation, Matsushita Electric Industrial Co., RIEKN)

P3-51 Molecular mechanism of self-assembly and polymorphic supercoiling of the bacterial flagellar filament based on the

atomic structure

K. Imada, F.A. Samatey, K. Hasegawa, S. Maki, K. Yonekura, I. Yamashita, K. Namba (Japan Science and Technology

Corporation, Matsushita Electric Industrial Co.)

P3-52 Conformational transition and amyloid formation of human calcitonin

T. Tomizawa, A. Nosaka, S. Kidokoro, K. Soda (Nagaoka University of Technology, Nagaoka National College of

Technology)

Structural analysis

P3-53 Complex electron microscopy II—Quantification of the contrast and resolution enhancement

R.S. Danev, S. Sugitani, H. Okawara, K. Murata, K. Nagayama (National Institute for Physiological Sciences)

P3-54 Prototype development of an automatic structure analysis system for protein crystals using molecular replacement

M. Kusunoki, K. Kinoshita, H. Sugawara, G. Kurisu, R. Hirose, N. Tanaka (Osaka University, RIKEN, Japan Science

and Technology Corporation, Tokyo Institute of Technology)

P3-55 Structural analysis by partial isotope labeling with modified inteins

N. Ito, T. Yamazaki, H. Nakamura (Biomolecular Engineering Research Institute, Osaka University)

XXI

P3-56 Segmental isotope labeling of protein

T. Otomo, T. Yamazaki (Osaka University)

P3-57 Structural analysis of thiol modified ft -lactoglobulin by using NMR

H. Kashiwai, K. Sakai, K. Sakurai, M. Hoshino, Y. Goto (Osaka University)

P3-58 Structural analysis of the cytokine LECT2

M. Ito, K. Nagata, Y. Kato, S. Yamagoe, K. Suzuki, M. Tanokura (University of Tokyo, National Institute of Infectious

Diseases)

P3-59 Solution structure of a protease inhibitor POIA1

S. Yoshinaga, H. Sasagawa, A. Tamura, K. Akasaka, S. Kojima, K. Miura (Kobe University, Gakushuin University)

P3-60 Ca2*-induced structural change of striated adductor muscle troponin C from Akazara scallop

F. Yumoto, K. Nagata, M. Nara, W. Iwasaki, K. Adachi, H. Kagi, A. Konno, N. Nemoto, K. Nishita, T. Ojima, M. Tanokura

(University of Tokyo, Tokyo Medical and Dental University, Marine Biotechnology Institute, Varian Technologies

Japan, Hokkaido University)

P3-61 Three-dimensional structure of a NifS homologue: CsdB protein from Escherichia coli

T. Fujii, H. Mihara, T. Kurihara, N. Esaki, Y. Hata (Kyoto University)

P3-62 Active site hinge motion of folate-bound Escherichia coli dihydrofolate reductase revealed by high pressure NMR

R. Kitahara, H. Yamada, E. Ohmae, K. Gekko, K. Akasaka (Kobe University, Hiroshima University)

P3-63 X-ray crystallographic study of nitroreductase NfsB from £ coli

W.C. Lee, M.E.P. Murphy, W. Iwasaki, H. Hirai, S. Kimura, H. Sasaki, S. Zenno, K. Saigo, M. Tanokura (University of

Tokyo, University of British Columbia, Ooita Medical University)

P3-64 Structure analysis of Bombyx mori lysozyme by X-ray crystallography

A. Matsuura, T. Aizawa, M. Yao, N. Koganesawa, K. Masaki, M. Demura, H. Bando, L Tanaka, K. Kawano, K. Nitta

(Hokkaido University, Toyama Medical and Pharmaceutical University)

P3-65 Crystallization and preliminary X-ray analysis of 4- a -glucanotransferase from Thermococcus litoralis

H. Imamura, S. Fushinobu, M. Yamamoto, T. Kumasaka, B.S. Jeon, T. Wakagi, H. Matsuzawa (University of Tokyo,

RIKEN, Aomori University)

P3-66 X-ray crystal structure of DsrD from Desulfovibrio vulgaris Hildenborough

N. Mizuno, D.S. Hittel, S. Adachi, K. Miki G. Voordouw, Y. Higuchi (Kyoto University, The University of Calgary,

RIKEN)

P3-67 X-ray crystallography of xenobiotics degradation enzyme CumD

T. Saku, S. Fushinobu, M. Hidaka, H. Nojiri, H. Yamane, T. Omori, T. Wakagi (University of Tokyo)

P3-68 Crystal structure of a novel c/s-prenylchain elongation enzyme, undecaprenyl diphosphate synthase

M. Fujihashi, K. Fujikura, Y. Higuchi, Y.W. Zhang, T. Koyama, K. Miki (Kyoto University, Tohoku University, RIKEN)

P3-69 The three-dimensional structure of an oxidative-DNA repair enzyme, MutM, from extreme thermophile

M. Sugahara, T. Mikawa, T. Kumasaka, M. Yamamoto, R. Kato, K. Fukuyama, Y. Inoue, S. Kuramitsu (Osaka

University, RIKEN)

P3-70 Crystal structure of blasticidin S deaminase from Aspergillus terreus and its ES complex

M. Furuichi, T. Kumasaka, M. Kimura, M. Yamamoto, I. Yamaguchi (RIKEN)

P3-71 Crystal structure of the restriction endoribonuclease complexed with its substrate analogue

S. Yajima, T. Ogawa, S. Inoue, T. Nonaka, Y. Mitsui, H. Masaki, K. Osawa (Tokyo University of Agriculture, University

of Tokyo, Nagaoka University of Technology)

Structure-function relationship I —enzymes

P3-72 Analysis of reaction mechanism of extradiol type dioxygenase

Y. Takahashi, Y. Uragami, K. Sugimoto, T. Senda, Y. Mitsui (Nagaoka University of Technology)

XXIX

P3-73 Crystal structure of His49Glu mutant of NADH-cytochrome 4 reductase and its electron-transfer pathway

H. Nishida, S. Kimura, K. Miki, T. lyanagi (Hitachi Ltd., Himeji Institute of Technology, Kyoto University, RIKEN)

P3-74 Involvement of the C-terminal region of ribosomal protein L2 in 23S rRNA binding activity and peptidyl transferase

activity

T. Hayashi, A. Ohsawa, M. Kimura, N. Yamasaki (Kyushu University)

P3-75 Substrate recognition of an enzyme with broad substrate specificity

J. Ishijima, T. Yano, H. Kagamiyama, S. Kuramitsu (Osaka University, Osaka Medical College)

P3-76 Analysis of doubly glycosylated hen lysozyme

Y. Hashimoto, 0. Munemura, K. Masumoto, T. Ueda, T. Imoto (Kyushu University)

P3-77 Role of Arg-410 and Tyr-411 in human serum albumin for ligand binding and esterase-like activity

H. Watanabe, S. Tanase, T. Maruyama, M. Otagiri (Kumamoto University)

P3-78 C-terminal module-substituted chimera phospholipase A2 as a mutant for analyzing the mechanism of necrosis

T. Ogawa, K. Igarashi, K. Muramoto, M. Go, M. Ohno (Tohoku University, Nagoya University, Kumamoto Institute of

Technology)

P3-79 Microenvironmental analysis of nucleotide binding domain of F,-ATPase ft subunit

H. Yagi, T. Iwabuchi, K. Tozawa, K. Ozawa, M. Yoshida, H. Akutsu (Yokohama National University, Tokushima

University, Tokyo Institute of Technology, Osaka University)

P3-80 A new method for evaluating protease activity with fluorescence polarization measurement

H. Takahashi, M. Myouga, S. Kidokoro, K. Soda (National Institute of Bioscience and Human-Technology, Nagaoka

University of Technology)

Structure-function relationship II —other proteins

P3-81 Structure and function of human killer cell immunoglobulin (Ig)-like receptor

K. Maenaka, A. van der Merwe, T. Juji, T. Maenaka, Y. Shirakihara, D. Stuart, Y. Jones (National Institute of

Genetics, University of Oxford, Japanese Red Cross Central Blood Center)

P3-82 Observation for the redox state of human serum albumin

T. Hayashi, H. Imai, K. Nakamura, T. Negawa, M. Tomida, K. Kuwata, S. Era (Gift* University)

P3-83 Topology analysis of drug binding site (site I) on human serum albumin using site-directed mutagenesis technique

K. Nakajou, H. Watanabe, S. Tanase, T. Maruyama, M. Otagiri (Kumamoto University)

P3-84 Zinc-binding site of transition protein 2 from boar late spermatid nuclei

K. Akama, A. Sekine, S. Kojima, K. Miura, M. Nakano (Chiba University, Gakushuin University)

P3-85 Screening of amino acid residues of hemolytic lectin CEL-HI involved in carbohydrate binding

Y. Kouzuma, S. Tojo, M. Nakano, M. Kimura, N. Yamasaki (Kyushu University)

P3-86 New members of galectin LEC-7~11 in C. elegans are a novel type of metal-mediated crosslinkers

K. Hayama, J. Hirabayashi, K. Kasai (Teikyo University)

P3-87 Effects of O-glycosylation on the structure and the biological activity of calcitonin

M. Tagashira, H. lijima, Y. Isogai, M. Hori, S. Takamatsu, Y. Fujibayashi, K. Yoshizawa-Kumagaye, S. Isaka, K.

Nakajima, T. Yamamoto, T. Teshima, K. Toma (Asahi Chemical Industry, Fukui Medical University,

Peptide Institute, The Noguchi Institute)

P3-88 Determination of the location of disulfide bridges in the soluble region of thrombopoietin receptor

Y. Matsukura, T. Tamada, R. Kuroki (Kirin Brewery Co.)

P3-89 Search for and evaluation of the antibody variable region applicable to Open Sandwich ELJSA

T. Aburatani, H. Ueda, I.M. Tomlinson, T. Nagamune (University of Tokyo, MRC)

P3-90 Intracellular peptide as biochemical regulator

T. Tsuchiya (Tokyo Medical and Dental University)

XXX