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Glen Mmanagthe CenBloedelfor the Vfaculty British Cmicrosc

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nd ProtectL. Corke1,3, DS, 2Dept. of versity of W

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Dr. WarrCornell Biophysnationaoptical multiphimagingWatt WincludinendoscSpectro OptimizWarren Dept. of Multipholiving spemodality traffickingparameterlevel of instill limitetalk will pdevelopm

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NeuroaTigriopuDavid RNeurosc

Arthropodiversitysuccess osalient enwork on and whicthat onlycharacterconcept tmajor arhowever,systems fsuch taximportangenotype1mm epoaddition,associateelaboratisuggest protocereprojectioTigriopuanatomic NeurontranspopituitaryJeremiaLowry2, 1 Arizon

Organic transpormany orgmonoamduring aregulatinhypothalstress re

anatomicaus californiR. Andrew cience, Un

ods are the my of morphoof arthropodnvironmentaarthropod

ch differentiy a subset ofrs are the bto arthropodthropod gro have been for a variety

xon, the marnt taxon e/phenotypeoxy sections we used c

ed neuropil. ons in the bevolutionaryebral bridge

ons from thes, and by excal complexi

al and gliaorter 3 (OCy-adrenal ah MolinarKenneth Ra State Un

cation trater whose sugans, includine transporn acute stre

ng the hylamus (DMHsponse and

Stud

l characteicus, a minand Nicho

niversity of

most specieological formds is due, in al stimuli annervous sysiated arthrof arthropod

basis for prod nervous syoups. Certaioverlooked

y of reasons.rine copepod

for stude interactions to produceconfocal mic

Despite thrain of Tigry affiliationse, the presense glomerul

xtension othity and sugg

al localizatT3) in hiera(HPA) axis

ro1, Luke MRenner3 niversity, 2U

nsporter 3 ubstrates inc

ding the brairter inhibiteess responseypothalamic-H), to increthereby reg

dent aerization ofnute crustaolas J. StraArizona

s-rich groupm, behaviorpart, to thei

nd tailor behstems revealopods from ods possessedoposing evoystems has in evolutionaas candidate. This studyd Tigriopus ies in p

n, and a cande 3D reconscroscopy to heir diminutriopus compas. These elnce of deutli to a centerher copepodsgests an anci

tion of corarchical sys in rats.

Mumaw1, S

University o

(OCT3) isclude norepin. OCT3-med by physioe. We hypoth-pituitary-adease extracegulate HPA a

abstraf the marinacean withusfeld

p of Metazor, and ecoloir nervous syhavioral respled certain aother metaz. The field lutionary reprovided insarily and ecoes for neuroy examines t

californicus, population didate for whstructions oreveal stru

tive size (~arable to muaborations i

terocerebral r in the laters, to malacoent origin o

rticosteronystems reg

ean Barto

of Colorado,

s a corticoinephrine, se

mediated tranological levehesize that Odrenal (HPellular monoaxis activity

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acts ne harpacth an elabo

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of cladisticelationships sights into tologically imoanatomical the neuroana a species tgenetics,

hole-genomeof the nervouctural detai1 mm at much larger minclude a ceglomeruli,

ral protocereostracan cruf these trait

e-sensitivegulating th

n1, Miles O

, 3University

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cation alamic-

Christoph

Dakota

tive, polyspand is locat

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ogram

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7  

insensitiv(PMAT) terminalprevious brain, reused duaand confthe HPAependymfunctionais currenin real-tislice prep Immuno1a (GHSS.E. DeaUniversi It is welmetabolighrelin htypically low), we Indeed, sin the prreproducdespite twithin bGHS-R1isthmus, immediatsimultanin 10% foimmunohthe reproof the recell whilevidence dairy cator indireenergy b

ve polyspecand OCT1

s, especially work has

search charaal immunoflufocal microscA axis in amal cells as al role of th

ntly unclear. me, OCT3-mparations wi

olocalizatiS-R1a) in thaver, P.B. Hty of Arizo

ll known thsm impacts

has been idenexperience postulate th

studies in roesence of gh

ctive tract ofthis initial covine reproa proteins wuterine bod

tely followeously as po

formalin for histochemicaoductive tisseproductive le GHS-R1afor the pre

ttle. Such wiect actions obalance that

ific transpoand OCT2 in regions

described thacterizing thuorescence ocopy to idendult male Swell as pre

his CORT-seAdditionally

mediated traith confocal

on of Ghrehe ReprodHoyer, S.Mna, Tucson

hat energy sreproductiv

ntified as a pa period of

hat ghrelin idents have dhrelin. Ghref several spcharacterizaoductive tisswithin the redy, corpus l

wing exsangositive contr24 hrs then

al techniquesues. Positivtract and tha was local

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rters such in regions owith few sphe regional he specific cof OCT3 andntify cell typSprague-Dasumed non-

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elin and thductive Tra. Dial, R.J. n, AZ

status influeve success apotent regulnegative enes involved indemonstrateelin and its aecies includi

ation, ghrelinsues. The aieproductive luteum, andguination. rols for ghren embedded es were usedve staining fhe control tislized to thehrelin and Gistribution inte or early eexperience d

as the plasof the brain

pecific presyndistribution

cell types thd markers opes that expwley rats. W-monoamine-monoaminedeveloped a nhe fluorescen

e Growth ct of DairyCollier and

ences reprodare not well lator of eneergy balancen the metaboed potent inhactive recepting humans,n and GHSm of this etract of dai

d follicle) wDuodenum

elin and GHin paraffin

d to identifyfor ghrelin assues. Ghrele plasma mGHS-R1a prndicates thaembryo; parduring early

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press OCT3We found

ergic neuronergic neuronnovel technint substrate

Hormone y Cattle. d M.L. Rho

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S-R1a proteexperiment iry cattle. Re

were harvestand hyp

HS-R1a, respfor microsc

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ane monoamnnervated byptake transp3 mRNA anOCT3 has ba (GFAP) or in brain rethat OCT3

nal soma innal clearanceique to obsee 4-di-2 ASP

Secretago

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t the mechad. The orexstasis. Givenrly lactation ion of reproearly embryo1a have beenp, and dairyins have nowas to loca

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nd GHS-R1a1a was evid

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ould affect feuring the pe

eting 2011 ‐ pr

mine transpy monoaminporters. Althnd protein inbeen limited.r neurons (Negions regul is localize the DMH.e of monoamerve and quaP (ASP+) in

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NeuN) lating ed on . The mines antify, acute

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R. Stowers, wZinsmaier. Tcausing supeutations, weon of a mutbes of these

antibodies t

fication of an ObjectivM. Hicks1, WArizona Coersity – Ariz

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chemopreectral dom Amber Lu

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the typicae of the and various asstissue morphsion using oT endoscopy olution, we h-points on smine if tre adenoma

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