PROFORMA FOR SUBMISSION OF RESEARCH PROJECTS · 203 Priority Area Forage production in problematic...
Transcript of PROFORMA FOR SUBMISSION OF RESEARCH PROJECTS · 203 Priority Area Forage production in problematic...
RPF-I
PROFORMA FOR SUBMISSION OF RESEARCH PROJECTS
PART -I: GENERAL INFORMATION
200 Project code
2001 Institute Code No CP.3.1.2
2002 ICAR Code No:
201 Name of Institute and Division:
2011 Name & Address of Institute:
2012 Name of Division/ Section:
2013 Location of Project:
202 Project Title
Indian Grassland and Fodder Research
Institute (IGFRI), Jhansi 284 003
Division of Crop Production
CP Division, IGFRI, Jhansi
Development of Phosphate Solubilizing
Biofertilizers Suitable for Fodder Crops of Problem Soils
203 Priority Area Forage production in problematic soils
2031 Research Approach:
Applied res Basic Res Process/Techno logy Transfer ofDevelopment Technology
obi 02-../ 03 04204 Specific areas Development of Phosphate Solubilizing
Biofertilizers Suitable for Fodder Crops of acid and salt affected soils
2041 Previous projects in this specific area:NONE
205 Duration: Five years
2051 Date of Start July, 2013
2052 Likely date of completion June, 2018
206 Total cost of the project: Rs. 93.65 lakhs
2061 Foreign Exchange Component (if any): Nil
207 Project profile Summary:
Out of the 157 Mha of the cultivated area 49 mha is acidic, of which 26 mha is
having pH <5.6 and the rest vary from 5.6 to 6.5 and area under salt-affected
soils is 6.73 M ha (Sodic - 3.77 Mha & Saline - 2.96 Mha). All the acid and salt
affected soils are constrained by deficient supply of soluble P. The application of
2
phosphatic fertilizers In acid soils form insoluble phosphate with AI3+, Fe3+,
while in calcareous soils, form insoluble calcium phosphate compounds.
Microbial solubilization of insoluble phosphates and RP is an environmentally
mild approach. The establishment and performance of PSMs is severely affected
by environmental factors especially under stressed conditions such as high salt,
pH and temperature prevalent in degraded ecosystems like alkaline soils.
Application of phosphate solubilizing microbial (PSM) biofertilizers prepared
out of strains isolated from normal soils do not perform well in problem soils due
to stress conditions. Very little information is available on the MPS
microorganisms of problem soils especially under acid and salt-affected soils.
Hence, it is highly necessary to study the MPS microorganisms of salt-affected
and acid soils to develop mineral phosphate solubilizing biofertilizers suitable
for fodder production in acid and salt affected soils.
208 Key words:
Acid soils, salt affected soils, forage crop, Phosphate solubilizers, biofertilizers,
Mineral phosphate solubilization.
Part -II: Investigator Profile
210 Principal Investigator
2101 Name : Dr Srinivasan R
: Senior Scientist
: Division of Crop Production
: IGFRI, Jhansi
: Indian Grassland and Fodder Research Institute, Jhansi
2102 Designation
2103 Division/ Section
2104 Location
2105 Institute Address
211 Co- Investigator
2111 Name
2112 Designation
2113 Division/Section
2114 Location
2115 Institute Address
212 Co-Investigator
2121 Name
: Mr. Manoj Chaudhary
: Scientist
: Division of Crop Production
: IGFRI, Jhansi
: Indian Grassland & Fodder Research Institute, Jhansi.
: Mr. Radhakrishna A
3
/ J.•..
Application of PSB had increased seed set (8.2 - 20.0%), seed filling efficiency
(1.3 - 1.9%), kernel yield (37.3 - 67.0%) and quality by reducing hull % (15.2 -
24.3) and by improving nutrient concentrations of sunflower seeds. Besides, PSB
application caused a substantial increase in all macro- and micro-nutrients in
seeds (Ekin, 2011). Inoculating thermo-tolerant PSMs into agricultural and
animal wastes represents a practical strategy for preparing multifunctional
Biofertilizer (Chang and Yang, 2009). Identification and phylogenetic analysis of
36 PSB isolates was done by 16S rDNA sequencing. four strains viz.,
Arthrobacter ureafaciens, Phyllobacterium myrsinacearum, Rhodococcus
erythropolis and Delftia sp. were reported for the first time as PSB (Chen et al.,
2006).
The highest records of all tested parameters were recorded with rock phosphate
inoculated with Paenibacillus polymyxa plus Bacillus megaterium treatment (El-
Yazeid and Abou Aly, 2011). Seed inoculation by PSMs and different levels of
phosphorus chemical fertilizer on forage and grain quality of barley had
decreased neutral detergent fibers (NDF) (57.4%) in barley forage (Mehrvarz
and Chaichi, 2008).
Biofertilizer named BioPower has exerted a positive influence by increasing the
accumulation of K+and Ca2+ ions for minimizing the deteriorating effect ofNaCl
salinity (Chugtai et al., 2003). Another Biofertilizer, Biogene, mixed to the soil,
improved the growth, performance and chemical constituents of both salt-
stressed and unstressed soyabean plants (Moftah, 2000).
221 Project Technical profile:
2211 Organization of work elements (for objective and participating investigator
giving man-months involved):
S. Name of the % time Work to be done
No. Scientist (man
months)
8
Dr. Srinivasan R 30 (4) Overall responsibility of project planning,
implementation of technical programme and
reporting. Microbiological aspects of
complete programme.
2. Mr. Manoj 20 (2.5)
Chaudhary
Soil analytical aspects of soil samples, Pot
and field experiment and analysis of soil and
plants sample for the mentioned
observations.
Molecular characterization for identification3. Mr. 20 (2.5)
Radhakrishna A of PSB and PSF; and Elucidation of possible
mechanism of MPS activity and stress
tolerance of the selected isolate
2212 Methodology:
Different salt affected and acid soils will be collected from various parts of India and
isolation of phosphate solubilizing microorganisms will be isolated. The isolates will be
screened for their phosphate solubilizing ability by using various P sources. About 20
PSB and PSF isolates will be selected for screening for various stress tolerance abilities
like acid tolerance, salt tolerance, drought tolerance, temperature stress tolerance. The
selected isolates will be also screened for any additional plant growth promoting.
substances production potential. Selected isolates will be characterized and identified by
morphological, biochemical and molecular based methods. Attempt will also be made to
elucidate possible mechanism of MPS activity and stress tolerance of the selected
isolate. 5 efficient salt and acid tolerant PSB and PSF isolates will be selected and tested
for its effect on growth of Fodder crop in salt affected soil (Barley) and in acid soil
(Oats) under Pot culture condition.
2213 Plan of action
2214 Time schedule of Activities (Milestones):
9
SI. Activities Responsible Years*Milestones
No organization 1st 2nd 3rd 4th 5th
1 Isolation of MPS micro Literature search and project out line Crop ~
organisms from the acid ProductionCollection of soil samples and soil ~ ~
and salt affected .soils Division 0
characterization and isolation ofPSMsIGFRIfrom different regions
~ Screening the efficiency Screening for mineral phosphate ~ ~
of the isolated strains for solubilizing activity
MPS activity, stress Screening of MPS isolates for various stress ~ ~ ~
olerance and other olerancebeneficial traits Screening selected isolates for possession of ~ ~
additional PGPR traits
~ Characterization and Characterization and identification of ~ ~ ~
identification of selected selected isolates
isolates by Elucidation of possible mechanism of MPS ~ ~ ~ ~.morphological, activity and stress tolerance of the selectedbiochemical and isolatemolecular methods
4 Testing the performance Effect on plant growth ~ ~
of selected efficient
strains for productivity of iAnalysis of soil and plant samples ~ ~
forage crops In in-vitro Field experiment ~ ~
conditionAnalysis of soil and plant samples ~ ~
Reporting the results ~
* In case of the unforeseen reasons if some of the activities are left duration may be
extended to the 6th year.
2215 Annual target of each activity:
Year wise plan of activity is given below and as mentioned above
10
First year:
Isolation of MPS microorganisms
Collection of soil samples for isolation of PSM
• Salt affected soils from Haryana (Kamal), Rajasthan / M. P. (Indore), T.
N. (Coastal areas, Chennai) - about 10 samples from each location
Acid soils from Kerala / Karnataka (Western Ghats), Himachal/Greater
Himalayas, West Bengal (Sundarbans) / NEHR.
Characterization of soil samples by standard analytical methods
pH,EC,ESP
• Isolation ofPSM using Pikovskaya's (1948) medium (PSB and PSF)
Second-third year:
Continuation of first year activity
Screening for MPS activity of isolates
• In Pikovskaya's broth medium modified with different P sources following
standard method (Jackson, 1973)
• TCP
• Al P04
FeP04
• Rock Phosphate (URP)
Select a total of about 20 PSB and 20 PSF having (High, Medium and low activities)
Screening for various stress tolerance of selected PSM isolates by testing the growth in
broth medium amended with salt or acid or PEG6000
• Salt tolerance range (Low, Medium, High)
• Acid tolerance range (Low, Medium, High)
• Drought tolerance (Low, Medium, High)
• Temperature tolerance (Low, Medium, High)
Characterization and identification of selected isolates
• Morphological (colony morphology, cell morphology (Gram staining and otherstaining methods)
• Biochemical (Different sugar utilization, and standard biochemical methods asper Bergey's manual and/or FAME analysis for PSB)
11
• Molecular methods (16S rDNA gene sequencing/ ARDRA for PSB and 18SrDNA gene sequencing! ARDRA / ITS gene based identification for PSF)
Screening of selected isolates for possession of additional PGPR traits if any,
Production of plant growth promoting substances like IAA, GA production using
standard methods
Elucidation of possible mechanism of MPS activity and stress tolerance of the selected
isolate
Biochemical (Chromatography / protein profiling) and/or
Molecular methods (based on identity of isolate, and its reported possible
expressed gene sequences, by PCR based method)
Fourth/fifth Year
••
Effect on plant growth
• Selection of 5 efficient salt tolerant PSB and PSF isolates for Pot cultureexperiment to test for effect on growth of Fodder crop in salt affected soil(Sorghum/Barley)
• Selection of 5 efficient acid tolerant PSB and PSF isolates for Pot cultureexperiment to test for effect on growth of Fodder crop in acid soil(Sorghum/Oats)
Treatments
Testing the performance of selected efficient strain(s) for P uptake and growth of
selected fodder crop under in vitro/in vivo condition.
In Salt affected soil - fodder crop (SorghumlBarley)
PSB-5
PSF -5
Reference culture - 2
VIC Control- 3 (RP, SSP, Absolute control)
In Acid soil - fodder crop (Sorghum/Oats)
PSB ., 5
PSF-5
Reference culture - 2
VIC Control- 3 (RP, SSP, Absolute control)
Observations
12
J
v
• Soil parameters to be studied - pH, EC, CEC, ESP, Ca, Mg, Na, K, organic C,moisture content, bulk density, available and total P
• Microbial population dynamics of soil
• Plant parameters - plant height, weight, shoot length, root length, girth, drymatter, plant P
Reporting the results
2216 Estimated Man months
man months
a. Scientific 9
b. Technical 4
c. Supporting 4
Proposed Research Details
Importance of the proposed project (Gaps in Knowledge / products/ process
technology) to the Institute mandate:
Proposed project will address the issues related to performance of PSM
biofertilizers in forage production under the problem soils, an important component of
the Institute mandate. Forage production under acid and salt affected soil. conditions are
constrained by low P availability, due to high P fixation. Such factors led to high
application of costly fertilizers to get desired level of production. In the proposed project
man months
man months
attempt will be made to develop the PSM biofertilizer to increase the bioavailability of
the native soil P reserve and to exploit the use of rock phosphate as P fertilizer along
with PSM biofertilizer.
Questions Attempted to be answered:
• How to increase the bioavailability of the native soil Preserve?
• How the efficiency of P sources can be increased in forage based cropping
system?
• How to address the problem of less/non performance of Biofertilizer of normal
soil origin in problem soil?
• What is the possible mechanism of P solubilization and stress tolerance in such
organisms?
• What are the possibilities of utilizing indigenous P sources or rock phosphate to
maintain desirable P balance in soil?
13
rl~
I
,,-.
PART - V: DECLARATION
This is to certify that
the research work proposed in the scheme / project does not in any way duplicate
the work already done or being carried out in the Institute on the subject.
the same project has been / has not been submitted to any other agency (ies) for
financial support.
the investigator/ Co investigators have been fully consulted in the development
of project and have fully undertaken the responsibility to carry out the
programme as per the technical programme.
~~'~ l' \~. . \~Srinivasan R)
Principal Investigato
( adhakrishna A)Co - Investigators
~~'1\\~(Manoj Choudhary) .
Co - Investigators
Signature & Comments of theJoint Director (Research)
~~~c:\,~~\~ t
Signature & Comments of theDirector