PRODUCTS MATERIALS - Sciencescience.sciencemag.org/content/sci/273/5280/1415.full.pdfcations include...
3
Biomolecule Purification and Analysis The VISION Workstation chromatography system offers simultaneous biomolecule puri- fication and analysis in a single instrument. Designed to reduce the delays caused by anal- ysis in the development laboratory, the Work- station combines proprietary Perfusion Chro- matography technology with novel sample handling robotics to accelerate the entire biomolecule purification process. The unit allows the user to perform separations of bio- molecules, then automatically re-inject the collected fractions for analysis by a variety of analytical techniques. PerSeptive Biosys- tems. Circle 139. Image Analysis Software GelBase/GelBlot Pro Image Analysis Soft- ware is designed for analysis of nucleic acid gels, autoradiographs, protein gels, spots, and blots. It is designed for one-dimensional use with ethidium bromide gels, Coomassie blue- or silver-stained gels, and autoradio- graphs. Images can be enhanced, zoomed, and printed. Features include a built-in stan- dard library for molecular weight determi- nations, automatic or manual background subtraction, automatic or manual peak iden- tifications, automatic histogram generation, automatic or manual area and volume calcu- lations, and optical density calculations with calibration standards for polymerase chain reaction work. Results tables include peak number, molecular weight calculations, area, height, position, volume, and amount of sam- ple. Data can be exported to most desktop processing programs. IBM-compatible and Macintosh versions are available. UVP. Circle 140. Chromosome Probe The Chromosome X/Y Cocktail Probe com- bines in one vial an X alpha satellite and a Y classical satellite probe. The cocktail probe can be used on a variety of samples including peripheral blood, bone marrow, tissue sec- tions, buccal smears, and amniocytes. Appli- cations include sex-mismatch transplant re- search, sex chromosome counting, and cen- tromere analysis. Results can be obtained in less than an hour. Oncor. Circle 141. Biocompatible Sample Extraction Column BioTrap 500 C18 is a biocompatible sample extraction column enabling repeated direct injections of plasma, serum, milk, supema- tants of cell cultures, fermentation broth, and other complex matrices into a high- performance liquid chromatography system without clean-up procedures other than a simple centrifugation. Repeated injections of up to 500 ,ul plasma can be made directly onto the BioTrap 500 C18 column. Proteins and other macromolecules can be totally removed from the sample before analysis of the drug molecules on the analytical column. Detection methods such as ultraviolet, fluo- rescence, electrochemical detection and mass spectrometry can be used. Advanced Separa- tion Technologies. Circle 142. U Vacuum Concentrator The Eppendorf Model 5301 Vacuum Concen- trator is for concentration of DNA/RNA, nu- cleotides, proteins, and other samples. It has a narrow width (less than 13 inches) and two stackable rotors for up to 96 x 1.5-ml to 2.0-ml -0 0* NEW! Endothelial NOS Products cDNA Probe, Specific Polyclonal Antibodies Electrophoresis Standards Inducible NOS and Neuronal NOS enzymes have been carefully puri- fied (>95%) to exclude all unrelated proteins and denatured to create our new electrophoresis standards. Active enzymes, along with specific antibodies for all NOS isoforms are also available from Cayman. Coming Soon! Improved Nitrate/Nitrite Assay Ultra sensitive fluorometric detec- tion of total NO. The fluorescent version of our popular colorimetric Nitrate/Nitrite Kit extends the lower limit of detection by 50 fold. In addition to these new products Cayman supplies a wide range of NO Inhibitors, Donors and Peroxynitrite. AYYMAN \ CHEMICAL 690 KMS Place, Ann Arbor, MI 48108 (800) 364-9897 * Fax: (313) 662-6896 E-mail: [email protected] Visit our website at www. CaymanChem. com Circle No. 11 on Readers' Service Card SCIENCE * VOL. 273 * 6 SEPTEMBER 1996 PRODUCTS & MATERIAL S Uw Newly offered instrumentation, apparatus, and laboratory materials of interest to researchers in all disciplines in academic, industrial, and government organizations are featured in this space. Emphasis is given to purpose, chief characteristics, and availability of products and materials. Endorsement by Science or AAAS is not implied. Addi- tional information may be obtained from the manufacturers or suppliers named by circling the appropriate number on the Readers' Service Card and placing it in a mailbox. Postage is free. 1415 0 0 on February 5, 2020 http://science.sciencemag.org/ Downloaded from
Transcript of PRODUCTS MATERIALS - Sciencescience.sciencemag.org/content/sci/273/5280/1415.full.pdfcations include...
BiomoleculePurification and Analysis
The VISION Workstation chromatographysystem offers simultaneous biomolecule puri-fication and analysis in a single instrument.Designed to reduce the delays caused by anal-ysis in the development laboratory, the Work-station combines proprietary Perfusion Chro-matography technology with novel sample
handling robotics to accelerate the entirebiomolecule purification process. The unitallows the user to perform separations of bio-molecules, then automatically re-inject thecollected fractions for analysis by a varietyof analytical techniques. PerSeptive Biosys-tems. Circle 139.
Image Analysis SoftwareGelBase/GelBlot Pro Image Analysis Soft-ware is designed for analysis of nucleic acidgels, autoradiographs, protein gels, spots,and blots. It is designed for one-dimensionaluse with ethidium bromide gels, Coomassieblue- or silver-stained gels, and autoradio-graphs. Images can be enhanced, zoomed,and printed. Features include a built-in stan-dard library for molecular weight determi-nations, automatic or manual backgroundsubtraction, automatic or manual peak iden-tifications, automatic histogram generation,automatic or manual area and volume calcu-lations, and optical density calculations withcalibration standards for polymerase chainreaction work. Results tables include peaknumber, molecular weight calculations, area,
height, position, volume, and amount of sam-ple. Data can be exported to most desktopprocessing programs. IBM-compatible andMacintosh versions are available. UVP.Circle 140.
Chromosome ProbeThe Chromosome X/Y Cocktail Probe com-bines in one vial an X alpha satellite and a Yclassical satellite probe. The cocktail probecan be used on a variety of samples includingperipheral blood, bone marrow, tissue sec-tions, buccal smears, and amniocytes. Appli-cations include sex-mismatch transplant re-search, sex chromosome counting, and cen-tromere analysis. Results can be obtained inless than an hour. Oncor. Circle 141.
Biocompatible SampleExtraction Column
BioTrap 500 C18 is a biocompatible sampleextraction column enabling repeated directinjections of plasma, serum, milk, supema-tants of cell cultures, fermentation broth,and other complex matrices into a high-performance liquid chromatography systemwithout clean-up procedures other than asimple centrifugation. Repeated injectionsof up to 500 ,ul plasma can be made directlyonto the BioTrap 500 C18 column. Proteinsand other macromolecules can be totallyremoved from the sample before analysis ofthe drug molecules on the analytical column.Detection methods such as ultraviolet, fluo-rescence, electrochemical detection and massspectrometry can be used. Advanced Separa-tion Technologies. Circle 142.
U
Vacuum ConcentratorThe EppendorfModel 5301 Vacuum Concen-trator is for concentration ofDNA/RNA, nu-cleotides, proteins, and other samples. It hasa narrow width (less than 13 inches) and twostackable rotors for up to 96 x 1.5-ml to 2.0-ml
-0
0*
NEW!Endothelial NOS ProductscDNA Probe, Specific PolyclonalAntibodies
Electrophoresis StandardsInducible NOS and Neuronal NOSenzymes have been carefully puri-fied (>95%) to exclude all unrelatedproteins and denatured to createour new electrophoresis standards.Active enzymes, along with specificantibodies for all NOS isoforms are
also available from Cayman.
Coming Soon!Improved Nitrate/Nitrite Assay
Ultra sensitive fluorometric detec-tion of total NO. The fluorescentversion of our popular colorimetricNitrate/Nitrite Kit extends the lowerlimit of detection by 50 fold.
In addition to these new productsCayman supplies a wide range of NOInhibitors, Donors and Peroxynitrite.
AYYMAN
\ CHEMICAL
690 KMS Place, Ann Arbor, MI 48108(800) 364-9897 * Fax: (313) 662-6896E-mail: [email protected]
Visit our website at
www. CaymanChem. com
Circle No. 11 on Readers' Service Card
SCIENCE * VOL. 273 * 6 SEPTEMBER 1996
PRODUCTS &MATERIALS
Uw
Newly offered instrumentation, apparatus, and laboratory materials of interest to researchers in all disciplines inacademic, industrial, and government organizations are featured in this space. Emphasis is given to purpose, chiefcharacteristics, and availability of products and materials. Endorsement by Science or AAAS is not implied. Addi-tional information may be obtained from the manufacturers or suppliers named by circling the appropriate numberon the Readers' Service Card and placing it in a mailbox. Postage is free.
1415
0
0
on February 5, 2020
http://science.sciencem
ag.org/D
ownloaded from
KINETICCITY-
The CoolestKids' Showon Radio,Now on
Tape!
The Kinetic City SuperCrew is a group of curiouskids that travel the worldin their express train to
solve brain-teasingscience mysteries!
Order a copy of the SuperCrew's latest adventure"Concrete Jungle or TheCase of the City Safari"and discover the wild
side of the city.To order, call toll-free1-888-GET-CREW
$8.95plus $4 s&h (per order)
Check out our Website for yourlocal KCSC station, and forother cool Super Crew stuff.http://www.aaas.org/EHR/KC
super.html
Kinetic City Super Creuw is a production of the AmericanAssociation for the Advancement of Science.Funding is provided by the National Science
Foundation and support from other contributors.
or 144 x 0.5-ml microcentrifuge tubes. Anautomatic vacuum release prevents sample lossdue to bumping and foaming. A braking fea-ture allows quick sample access. Aeration modemaximizes evaporation efficiency and pro-
longs pump life-span by periodically aeratingthe vacuum line and preventing condensa-tion buildup. The user has a choice of fourheating levels (300C, 450C, 600C, and ambi-ent). Brinkmann Instruments. Circle 143.
Sequence Softwareand Sequence Database
GeneJockey II is a program for editing, ma-
nipulating, and analyzing nucleic acid andprotein sequences. It can rapidly scan nu-
cleic acid sequences to select all possiblepairs of oligonucleotides suitable for use as
primers to direct DNA amplification by poly-merase chain reaction. Version 2 of the pro-
gram includes fast search routines of the Se-quence Database. Searches can be performedby browse sequence file, locus or accessionnumber, author, keyword and title indices,and more. The Sequence Database is a sepa-
rate product that incorporates all the infor-mation currently found in the flatfile formatof GenBank compressed onto a single CD-ROM. Biosoft. Circle 144.
Total Protein StainingISS Gold-Blot is a colloidal gold reagent fortotal protein staining on nitrocellulose or
polyvinylidine fluoride membranes with sen-
sitivity equal to or exceeding silver-stain sen-
sitivity in polyacrylamide gels. ISS Gold-Blotforms complexes with proteins to yield red-purple bands in only 30 min. It includes a
detergent blocking agent for minimal back-ground and ready-to-use stain reagent. Inte-grated Separation Systems. Circle 145.
Hybridizationand Detection Systems
The MaxSense line of products for specificmembrane hybridization and detection hasbeen optimized to provide the best hybridiza-tion and washing conditions for background-free, highly sensitive results with nonradio-active probes labeled with biotin, digoxigenin,or fluorescein haptens. The MaxSense Bio-Probe Hybridization System is designed foruse with DNA and RNA probes prepared bynick translation, random primed, or in vitroRNA transcript labeling procedures. TheMaxSense OligoProbe Hybridization Systemis for use with oligonucleotide probes pre-
pared by terminal labeling procedures. Thedetection systems offer either alkaline phos-
phatase- or horseradish peroxidase-linked de-tection reagents for biotin, digoxigenin, andfluorescein haptens. This unification of hy-bridization and detection reagents makes itsimple and straightforward to do multipledetections on a single blot. Enzo Diagnos-tics. Circle 146.
Electroporation SystemsThe EasyjecT Electroporation Systems pro-
vide improved set-up and programming ca-
pabilities for precise optimization and repro-
ducibility of transformations. The systems
are remote-control operated and equippedwith "Smart Card" reader and recorder mem-ory for simple and safe storage and recall ofall programs. The EasyjecT BASIC Systemprovides a completely programmed and opti-mized system for the transformation of Es-cherichia coli and other Gram-negative bacte-ria. Owl Scientific. Circle 147.
LiteratureThe Water Purification Primer provides a basicguide to the types of contaminants found inwater and the types of technologies used topurify water used in the laboratory. Tech-nologies described include distillation, ionexchange, carbon adsorption, microporousmembrane filtration, reverse osmosis, ultrafil-tration, ultraviolet radiation, and the patentedElectrodeionization. Millipore. Circle 148.
Wako for Your Life's Research features re-
search and analytical products including an-
tibodies and enzymes, cell culture and signaltransduction reagents, molecular biology andmicrobial detection kits, mammalian trans-fection products, fluorescent probes and la-beling reagents, analytical standards, stains,and other biochemicals. Wako BioProducts.Circle 149.
Harlan Sprague Dawley Product Guide offers240 stocks and strains of laboratory animals,including rats, mice, hamsters, guinea pigs,gerbils, cats, beagles, hounds, and miniatureswine. Harlan Sprague Dawley. Circle 150.
SCIENCE * VOL. 273 * 6 SEPTEMBER 1996
Li
1416
on February 5, 2020
http://science.sciencem
ag.org/D
ownloaded from
Products & Materials
DOI: 10.1126/science.273.5280.1415 (5280), 1415-1416.273Science
ARTICLE TOOLS http://science.sciencemag.org/content/273/5280/1415.citation
PERMISSIONS http://www.sciencemag.org/help/reprints-and-permissions
Terms of ServiceUse of this article is subject to the
is a registered trademark of AAAS.ScienceScience, 1200 New York Avenue NW, Washington, DC 20005. The title (print ISSN 0036-8075; online ISSN 1095-9203) is published by the American Association for the Advancement ofScience
© 1996 American Association for the Advancement of Science
on February 5, 2020
http://science.sciencem
ag.org/D
ownloaded from
