PRODUCT CATALOGUE4.imimg.com/data4/IB/JP/MY-9824300/cl_orangeindia.pdf · 2020. 1. 16. · 2011 is...

PRODUCT CATALOGUE

Overview

ORANGE is an emerging organization with new innovations manufacturing Biotech Instruments both standard and customized products ,Teaching kits, Research kits, Biochemical and buffers, Plasmid and DNA , Markers (DNA & Protein) etc.

Projects and trainings are regulated by our quali�ed faculties with 100% safe and secure manner.Motto of the Organization is We Reach More…..

VisionInspiration from Customer Satisfaction

Pro�leBackground & Development2011 is the year when ORANGE had step in to this tremendous life science �eld. Astonishingly we have com-pleted these Four years af�ned by many Success and Learning Experiences. A Mutual relationship with customers had been building through eminent Products and Services.

We started with few representatives with their backbreaking work our organization has reached among several Colleges and Research Institutes. With art of manufacturing capability we have developed our Laboratory Instruments and Innovative techniques in teaching kits.

DepartmentsOur Entity is articulated by different Departments are, • Administration • Production• Instrumentation• Research and Development • Workshop , Trainings & Projects Development• Marketing • Other Service

Role• Manufacturer of Biotech Instruments, Teaching kits, Buffers and Reagents and provider of Technical Support.• Provider of Standard and customized products.

ABOUT USOverview

ORANGE is an emerging organization with new innovations manufacturing Biotech Instruments both standard and customized products ,Teaching kits, Research kits, Biochemical and buffers, Plasmid and DNA , Markers (DNA & Protein) etc.

Projects and trainings are regulated by our quali�ed faculties with 100% safe and secure manner.Motto of the Organization is We Reach More…..

VisionInspiration from Customer Satisfaction

Pro�leBackground & Development2011 is the year when ORANGE had step in to this tremendous life science �eld. Astonishingly we have com-pleted these Four years af�ned by many Success and Learning Experiences. A Mutual relationship with customers had been building through eminent Products and Services.

We started with few representatives with their backbreaking work our organization has reached among several Colleges and Research Institutes. With art of manufacturing capability we have developed our Laboratory Instruments and Innovative techniques in teaching kits.

DepartmentsOur Entity is articulated by different Departments are, • Administration • Production• Instrumentation• Research and Development • Workshop , Trainings & Projects Development• Marketing • Other Service

Role• Manufacturer of Biotech Instruments, Teaching kits, Buffers and Reagents and provider of Technical Support.• Provider of Standard and customized products.

ABOUT US

PRODUCT INDEX

Electrophoresis Units & Accessories

Horizontal Electrophoresis 2

Vertical Electrophoresis 11

Blotting Apparatus 17

Immuno Electrophoresis 28

Paper Gel Electrophoresis 30

Tube Gel Unit 31

2-D PAGE Gel Unit 32

Power Supply & Transilluminators

Power Supplies 34

UV Transilluminators 40

Portable UV Lamps 45

Other Lab Instruments

PCR work hood 47

TLC viewer 48

Gel Rockers 49

Table Top pH meter 50

Colony counter 51

Gel Doc System 52

New Arrivals 161

Lab Chemicals & Kits Extraction Kits 53

Teaching Kits 61

Molecular Biology

Genomics Techniques 64

Proteomics Techniques 84

Transcriptomics Techniques 93

Immunology

Agglutination Techniques 96

Chromatography Techniques 99

ELISA Techniques 101

Hematology Techniques 103

Precipitation Techniques 107

Microbiology

Isolation & Identi�cation of Microbes 110

Growth Kinetic Techniques 111

Staining Techniques 113

Tissue Culture Techniques 116

Apoptosis 117

DNA , Plasmids & Vectors 118

DNA & Protein Markers 119

Enzymes 120

Buffers 123

Reagents 130

Biochemicals 144

Strains & Immunocomponents 152

Lab Wares 153

Academic Training Module 155

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ELECTROPHORESIS APPARATUS

Horizontal Immersed Gel Electrophoresis Unit & Accessories

Standard Model Horizontal Electrophoresis Unit

The most ef�cient way to separate DNA fragments in Agarose Gels is by "Submarine" electrophoresis. In this technique, the entire gel is submerged in the buffer.

We offer all different type of models for different applications like Junior,Mini, Midi, Maxi & Customized Electrophoresis etc.,

Speci�cation Junior Horizontal Mini Horizontal Midi Horizontal Maxi Horizontal Maxi Horizontal WC*

Cat.No

Dimensions (LxBxH)cm

Buffer Volume

No.of Samples

Gel Size in cm

Price (INR)

HJS0005

14.5x6.5x5.5

150ml

5

7x4

2530.00

HMS0008

18.5x9.5x7

300ml

8

7x5,10x7

4350.00

HDS0036

25.5x15.5x7

450ml

36

7x5,13x6,13x10

7810.00

HXS0060

25x23x7

900ml

60

20x15,20x20

9950.00

HWS0061

45x25x10

900ml

60

20x15,20x20

11990.00

Selection Guide - Standard Model Horizontal Electrophoresis Unit

Speci�cation Mini Horizontal Mini Super Horizontal Midi Horizontal Midi Super Horizontal Maxi Horizontal

Cat.No

Dimensions (WxLxH)cm

Buffer Volume

No.of Samples

Gel Size (WxL) in cm

Price (INR)

HMA0015

13x24 x6.5cm

325ml

15

10x7

5440.00

HSA0023

16.5x23 x6.5cm

400ml

23

10 x 11.5cm

6250.00

HDA0052

19.5 x 28.5 x 6.5cm

600ml

52

7x5,13x6,13x10

9760.00

HSA0086

21.5 x 33.5 x 7cm

1200ml

86

20x10,20x20cm

13430.00

HXA0162

33 x 56 x 9cm

2200ml

162

25 x 30cm

17470.00

Selection Guide - ACE Series Model Horizontal Electrophoresis Unit

Junior Horizontal Immersed Gel Electrophoresis Unit

Cat.No Qty Price/Pack (`)

1 2530.00HJS0005

Speci�cation:

WC*-Water Circulation

Make : ORANGEModel : Standard- Junior HorizontalCat.No : HJS0005Dimension : 14.5cm(L)x6.5cm(B)x5.5cm(H)Number of Samples : 5Buffer Volume : 150mlNumber of casting Tray : 7x4cm (1 No.)Number of Combs : 5-wells 1.5mm thickness comb (Well Width-5mm,Interspace-3mm) (2 Nos.) 4-wells 1.5mm thickness comb (Well Width-6mm,Interspace-4mm) (1 No.)Comb Capacity (5mm Deep Well) : 37μl,44μlConnecting Cord : Red and Black (Each One)No. of Platinum electrodes : Positive and Negative (Each One)Buffer Chamber with Safety Lid : 1 No.Gel Caster : Optional* (Extra Cost)Instruction Manual : 1 No.Warranty Card : 1 No.

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Accessories for Junior Horizontal

Cat.No Description Qty Price/Pack (`)

220.00

430.00

275.00

320.00

320.00

375.00

590.00

450.00

550.00

550.00

250.00

HJS0051 Casting Tray 7x4cm 1

HJS0052 Casting Tray 7x4cm (UVT) 1

HJS0053 4-wells 1mm thickness comb 1

HJS0054 4-wells 1.5mm thickness comb 1

HJS0055 5-wells 1mm thickness comb 1

HJS0056 5-wells 1.5mm thickness comb 1

HJS0057 Gel Caster for Junior 1

HJS0058 Gel Scoop Ordinary for Junior Gel 1

HJS0059 Gel Scoop UVT for Junior Gel 1

HJS0510 Platinum Electrode for Junior Single Side 1

HJS0511 Connecting Cord 1

Accessories for Mini Horizontal


290.00

570.00

450.00

890.00

390.00

450.00

650.00

720.00

850.00

250.00

290.00

820.00

250.00

1

1

1

1

1

1

1

1

1

1

1

1

1

HMS0081 Casting Tray 7x5cm

HMS0082 Casting Tray 7x5cm (UVT)



HMS0085 8-wells 1mm thickness comb

HMS0086 8-wells 1.5mm thickness comb

HMS0087 Gel Caster for Mini

HMS0088 Gel Scoop Ordinary for Mini Gel

HMS0089 Gel Scoop UVT for Mini Gel



HMS0812 Platinum Electrode for Mini Single Side

HJS0511 Connecting Cord

Mini Horizontal Immersed Gel Electrophoresis Unit


1 4350.00HMS0008

Speci�cation:

Make : ORANGE Model : Standard-Mini Horizontal Cat.No : HMS0008 Dimension : 18.5cm(L)x9.5cm(B)x7cm(H) Number of Samples : 8 Buffer Volume : 300ml Number of casting Tray : 7x5cm,10x7cm(1 No.Each) Number of Combs : 8-wells 1.5mm thickness comb (Well Width-5mm,Interspace-3mm) (2 Nos.) 3-wells 1.5mm thickness comb (Well Width-18mm,Interspace-4mm) (1 No.)Comb Capacity (5mm Deep Well) : 37μl,132μl Connecting Cord : Red and Black (Each One) No. of Platinum electrodes : Positive and Negative (Each One) Buffer Chamber with Safety Lid : 1 No. Gel Caster : Optional* (Extra Cost) Instruction Manual : 1 No. Warranty Card : 1 No.

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Accessories for Midi Horizontal


590.00

890.00

850.00

1250.00

590.00

650.00

790.00

850.00

1150.00

1020.00

1800.00

250.00

290.00

1650.00

250.00

290.00

570.00

450.00

890.00

390.00

450.00

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

HDS0391 Casting Tray 13x6cm

HDS0392 Casting Tray 13x6cm (UVT)

HDS0393 Casting Tray 13x10cm

HDS0394 Casting Tray 13x10cm (UVT)

HDS0395 13-wells 1mm thickness comb

HDS0396 13-wells 1.5mm thickness comb

HDS0397 18-wells 1mm thickness comb

HDS0398 18-wells 1.5mm thickness comb

HDS0399 Gel Caster for Midi

HDS3910 Gel Scoop Ordinary for Midi Gel

HDS3911 Gel Scoop UVT for Midi Gel



HDS3914 Platinum Electrode for Midi Single Side








Midi Horizontal Immersed Gel Electrophoresis Unit


1 7810.00HDS0036

Speci�cation:

Make : ORANGE

Model : Standard-Midi Horizontal

Cat.No : HDS0036

Dimension : 25.5cm(L)x15.5cm(B)x7cm(H)

Number of Samples : Upto 36

Buffer Volume : 450ml

Number of casting Tray : 7x5cm,13x6cm,13x10cm (1 No. Each)

Number of Combs : 8-wells 1.5mm thickness comb (Well Width-5mm,Interspace-3mm) (2 Nos.)

3-wells 1.5mm thickness comb (Well Width-18mm,Interspace-4mm) (1 No.)

13-wells 1.5mm thickness comb (Well Width-5mm,Interspace-5mm) (2 Nos.)


Comb Capacity (5mm Deep Well) : 37μl,132μl,30μl

Connecting Cord : Red and Black (Each One)

No. of Platinum electrodes : Positive and Negative (Each One)

Buffer Chamber with Safety Lid : 1 No.

Gel Caster : Optional* (Extra Cost)

Instruction Manual : 1 No.

Warranty Card : 1 No.

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Maxi Horizontal Immersed Gel Electrophoresis Unit


1 9950.00HXS0060

Speci�cation:

Make : ORANGE Model : Standard-Maxi Horizontal Cat.No : HXS0060 Dimension : 25cms(L)x23cms(B)x7cms(H) Number of Samples : Upto 60 Buffer Volume : 750ml Number of casting Tray : 20x15cm,20x20cm (1 No. Each) Number of Combs : 26-wells 1.5mm thickness comb (Well Width-4mm,Interspace-3mm) (2 Nos.) 30-wells 1.5mm thickness comb (Well Width-3mm,Interspace-3mm) (1 No.) Comb Capacity (5mm Deep Well) : 30μl,22μl Connecting Cord : Red and Black (Each One) No. of Platinum electrodes : Positive and Negative (Each One) Buffer Chamber with Safety Lid : 1 No. Gel Caster : Optional* (Extra Cost) Instruction Manual : 1 No. Warranty Card : 1 No.

Maxi Horizontal Immersed Gel Electrophoresis with Water Circulation Unit


1 11990.00HWS0061

Speci�cation:

Make : ORANGE Model : Standard-Maxi Horizontal Cat.No : HWS0061 Dimension : 45cms(L)x25cms(B)x10cms(H) Number of Samples : Upto 60 Buffer Volume : 750ml Number of casting Tray : 20x15cm,20x20cm (1 No. Each) Number of Combs : 26-wells 1.5mm thickness comb (Well Width-4mm,Interspace-3mm) (2 Nos.) 30-wells 1.5mm thickness comb (Well Width-3mm,Interspace-3mm) (1 No.) Comb Capacity (5mm Deep Well) : 30μl,22μl Connecting Cord : Red and Black (Each One) No. of Platinum electrodes : Positive and Negative (Each One) Buffer Chamber with Safety Lid : 1 No. Water Circulation Port : Provided Gel Caster : Optional* (Extra Cost) Instruction Manual : 1 No. Warranty Card : 1 No.

Accessories for Maxi Horizontal


1150.00

2200.00

1300.00

3900.00

1100.00

1150.00

1390.00

1450.00

1750.00

1750.00

2580.00

2680.00

250.00

1

1

1

1

1

1

1

1

1

1

1

1

1

HXS0601 Casting Tray 20x15cm

HXS0602 Casting Tray 20x15cm (UVT)

HXS0603 Casting Tray 20x20cm

HXS0604 Casting Tray 20x20cm (UVT)

HXS0605 26-wells 1mm thickness comb

HXS0606 26-wells 1.5mm thickness comb

HXS0607 30-wells 1mm thickness comb

HXS0608 30-wells 1.5mm thickness comb

HXS0609 Gel Caster for Maxi

HXS6010 Gel Scoop Ordinary for Maxi Gel

HXS6011 Gel Scoop UVT for Maxi Gel

HXS6012 Platinum Electrode for Maxi Single Side


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ACE Series Model Horizontal Electrophoresis Unit

ACE Series Mini Horizontal Immersed Gel Electrophoresis Unit


1 5010.00HMA0015

Speci�cation:

Make : ORANGE

Model : ACE Series-Mini Horizontal

Cat.No : HMA0015

Dimension : 13cm(L)x24cm(B) x6.5cm(H)

Number of Samples : 15


Number of casting Tray :7x5cm,10x7cm(1 No.Each)

Number of Combs : Multichannel Pipette Compatible Combs

Height Adjustable Combs

15-wells 1.5mm thickness comb (Well Width-3mm,Interspace-1.5mm) (1 No.)




Handle : Provided in the Unit




Gel Caster : 1 No.



Accessories for ACE Mini Horizontal


350.00

620.00

490.00

940.00

750.00

820.00

590.00

680.00

350.00

350.00

120.00

890.00

650.00

250.00

1

1

1

1

1

1

1

1

1

1

6/Pack

1

1

1

HMA0151 Casting Tray 7x5cm

HMA0152 Casting Tray 7x5cm (UVT)



HMA0155 15-wells 1mm thickness Height Adjustable comb

HMA0156 15-wells 1.5mm thickness Height Adjustable comb





HMA1511 Comb Screws

HMA1512 Platinum Electrode for ACE Mini Single Side

HMA1513 Gel Caster ACE Mini


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ACE Series Mini Super Horizontal Immersed Gel Electrophoresis Unit


1 6250.00HSA0023

Speci�cation:

Make : ORANGE

Model : ACE Series-Mini Super Horizontal

Cat.No : HSA0023

Dimension : 16.5cm(L)x23cm(B)x6.5cm(H)



Number of casting Tray :7x5cm,10x11.5cm (1 No.Each)



23-wells 1.5mm thickness comb (Well Width-3mm,Interspace-1.5mm) (1 No.)








Gel Caster : 1 No.



Accessories for ACE Mini Super Horizontal


350.00

620.00

490.00

940.00

750.00

820.00

590.00

680.00

350.00

350.00

120.00

890.00

650.00

250.00

1

1

1

1

1

1

1

1

1

1

6/Pack

1

1

1



HSA2301 Casting Tray 10x11.5cm

HSA2302 Casting Tray 10x11.5cm (UVT)

HSA2303 23-wells 1mm thickness Height Adjustable comb

HSA2304 23-wells 1.5mm thickness Height Adjustable comb





HMA1511 Comb Screws

HSA2305 Platinum Electrode for ACE Mini Super Single Side

HSA2306 Gel Caster ACE Mini Super


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ACE Series Midi Horizontal Immersed Gel Electrophoresis Unit


1 9760.00HDA0052

Speci�cation:

Make : ORANGE

Model : ACE Series-Midi Horizontal

Cat.No : HDA0052

Dimension : 19.5cm(L)x28.5cm(B)x6.5cm(H)



Number of casting Tray :7x5cm,13x6cm,13x10cm (1 No.Each)



26-wells 1.5mm thickness comb (Well Width-3mm,Interspace-1.5mm) (2 Nos.)





Comb Capacity (5mm Deep Well) : 22μl,37μl,30μl,132μl





Gel Caster : 1 No.



Accessories for ACE Midi Horizontal


350.00

620.00

690.00

990.00

950.00

1390.00

750.00

820.00

690.00

770.00

890.00

960.00

590.00

680.00

350.00

350.00

120.00

950.00

1190.00

250.00

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

6/Pack

1

1

1

HDA05251 Casting Tray 7x5cm

HDA05252 Casting Tray 7x5cm (UVT)





HDA05257 26-wells 1mm thickness Height Adjustable comb

HDA05258 26-wells 1.5mm thickness Height Adjustable comb









HMA1511 Comb Screws

HDA5213 Platinum Electrode for ACE Midi Single Side

HDA5214 Gel Caster ACE Midi


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ACE Series Midi Super Horizontal Immersed Gel Electrophoresis Unit


1 13430.00HSA0086

Speci�cation:

Make : ORANGE

Model : ACE Series-Midi Super Horizontal

Cat.No : HSA0086




Number of casting Tray : 20x10cm,20x20cm (1 No.Each) with Two slots






Comb Capacity (5mm Deep Well) : 22μl,30μl,





Gel Caster : 1 No.



Accessories for ACE Midi Super Horizontal


1150.00

2200.00

1300.00

3900.00

1450.00

1590.00

1390.00

1450.00

1100.00

1150.00

120.00

2750.00

1790.00

250.00

1

1

1

1

1

1

1

1

1

1

6/Pack

1

1

1

HSA0861 Casting Tray 20x10cm

HSA0862 Casting Tray 20x10cm (UVT)

HSA0863 Casting Tray 20x15cm

HSA0864 Casting Tray 20x15cm (UVT)







HMA1511 Comb Screws

HSA8611 Platinum Electrode for ACE Midi Single Side

HSA8612 Gel Caster ACE Midi


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Accessories for ACE Maxi Horizontal


1550.00

1890.00

1590.00

1650.00

1390.00

1450.00

1100.00

1150.00

120.00

2950.00

1980.00

250.00

1

1

1

1

1

1

1

1

6/Pack

1

1

1

HXA1621 Casting Tray 25x30cm

HXA1622 Casting Tray 25x30cm (UVT)

HXA1623 54-wells 1mm thickness Height Adjustable comb

HXA1624 54-wells 1.5mm thickness Height Adjustable comb





HMA1511 Comb Screws

HXA1629 Platinum Electrode for ACE Maxi Single Side

HXA6210 Gel Caster ACE Maxi


ACE Series Maxi Horizontal Immersed Gel Electrophoresis Unit


1 17470.00HXA0162

Speci�cation:

Make : ORANGE

Model : ACE Series-Maxi Horizontal

Cat.No : HXA0162

Dimension : 33cm(L)x56cm(B)x9cm(H)



Number of casting Tray : 25x30cm (1 No.) with Three Slots






Comb Capacity (5mm Deep Well) : 22μl,30μl,





Gel Caster : 1 No.



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Mini Vertical Gel Electrophoresis Unit (SDS - PAGE Apparatus)


1 6160.00VMR0007

Speci�cation:

Make : ORANGE

Model : Regular- Mini Vertical

Cat.No : VMR0007



Buffer Volume : 100+150ml

Gel Size : 8x7 (1 Gel)

Glass Plate : 10x10cm Notched and Square Plates (2 Sets)

Number of Combs : 7-wells 1mm thickness comb (Well Width-7mm,Interspace-3mm) (1 No.)


Spacers : 1mm and 1.5mm Spacers (3 Nos. Each)

Gasket : Fixed

Comb Capacity : 35μl,52μl

Clamp and Screws : 1 Set




Gel Casting System : 1 No.



Vertical ElectrophoresisVertical Gel Electrophoresis Systems & Accessories

Regular Model Vertical Gel Electrophoresis System( SDS - PAGE Apparatus)

ORANGE offers a complete range of vertical gel electrophoresis systems, suitable for a wide variety of modern electrophoretic techniques.The MINI model answers the small, conventional electrophoresis unit for fast, high resolution protein separations in 8 x 7 cm gel. The MIDI model is used for electrophoresis in 16 x 14 cm gel. The DUAL models are versatile ones for running two gels, under similar conditions simultaneously. Gels can be cast in two different thickness with the GEL CASTING UNIT, available for both MINI or MIDI,MAXI,MAXI Broad and DUAL models.

Speci�cation MINI Vertical MINI Dual Vertical MIDI Vertical MIDI Dual Vertical MAXI Vertical MAXI Broad Vertical

Cat.No


Buffer Volume

No.of Samples

Gel Size

Price (INR)

VMR0007

12x10x11

100+150ml

7

8x7cm (1 Gel)

6160.00

VTR0014

12x15x11

150+200ml

14

8x7cm (2 Gel)

9350.00

VDR0013

23x18x20

150+200ml

13

16x14cm (1 Gel)

10340.00

VDR0026

18x21x20

250+500ml

26

16x14cm (2 Gel)

13370.00

VXR0020

29x20x21

300+600ml

20

20x16cm (1 Gel)

16710.00

VBR0080

55x20x24

600+750ml

80

41x20cm (1 Gel)

21720.00

Selection Guide - Regular Model Vertical Gel Electrophoresis System

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450.00

420.00

490.00

550.00

450.00

480.00

540.00

470.00

2270.00

350.00

420.00

890.00

2900.00

250.00

Set

1

1

1

6/Pack

6/Pack

6/Pack

1

Set

1

1

1

Pair

1

VMR0071 Glass Plate 10x10cm (8x7cm Gel)

VMR0072 7-wells 0.5mm thickness comb

VMR0073 7-wells 1mm thickness comb

VMR0074 7-wells 1.5mm thickness comb

VMR0075 Spacers 0.5mm thickenss

VMR0076 Spacers 1mm thickenss

VMR0077 Spacers 1.5mm thickenss

VMR0078 Gel Caster Mini

VMR0079 Glass Plate,Comb and Spacers Combo

VMR0710 1mm thickness Acrylic Spacer

VMR0710 1.5mm thickness Acrylic Spacer

VMR0711 Gel Staininig Tray Mini

VMR0712 Platinum Electrode for Mini Vertical (Upper+Lower Tank)


Accessories for Mini and Dual Vertical (SDS - PAGE)

Mini Dual Vertical Gel Electrophoresis Unit (SDS - PAGE Apparatus)


1 9350.00VTR0014

Speci�cation:

Make : ORANGE

Model : Regular- Mini Dual Vertical

Cat.No : VTR0014






Number of Combs : 7-wells 1mm thickness comb (Well Width-7mm,Interspace-3mm)(2 Nos.)



Gasket : Fixed


Clamp and Screws : 2 Sets




Gel Casting System : 2 Nos.



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Midi Vertical Gel Electrophoresis Unit (SDS - PAGE Apparatus)


1 9700.00VTR0013

Speci�cation:

Make : ORANGE

Model : Regular- Midi Vertical

Cat.No : VTR0013









Gasket : Fixed









Midi Dual Vertical Gel Electrophoresis Unit (SDS - PAGE Apparatus)


1 12650.00VDR0026

Speci�cation:

Make : ORANGE

Model : Regular- Midi Dual Vertical

Cat.No : VDR0026




Gel Size : 16x14cm (2 Gel)


Number of Combs : 13-wells 1mm thickness comb (Well Width-6mm,Interspace-4mm) (2 Nos.)

13-wells 1.5mm thickness comb (Well Width-6mm,Interspace-4mm)(2 Nos.)


Gasket : Fixed


Clamp and Screws : 2 Sets




Gel Casting System : 2 Nos.



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690.00

750.00

790.00

850.00

750.00

780.00

795.00

690.00

3900.00

490.00

680.00

1450.00

4800.00

250.00

Set

1

1

1

6/Pack

6/Pack

6/Pack

1

Set

1

1

1

Pair

1

VDR0131 Glass Plate 18x18cm (16x14cm Gel)

VDR0132 13-wells 0.5mm thickness comb

VDR0133 13-wells 1mm thickness comb

VDR0134 13-wells 1.5mm thickness comb

VDR0135 Spacers 0.5mm thickenss

VDR0136 Spacers 1mm thickenss

VDR0137 Spacers 1.5mm thickenss

VDR0138 Gel Caster Midi

VDR0139 Glass Plate,Comb and Spacers Combo

VDR1310 1mm thickness Acrylic Spacer

VDR1311 1.5mm thickness Acrylic Spacer

VDR1312 Gel Staininig Tray Midi

VDR1313 Platinum Electrode for Midi Vertical (Upper+Lower Tank)


Accessories for Midi and Dual Vertical (SDS - PAGE)

Maxi Vertical Gel Electrophoresis Unit (SDS - PAGE Apparatus)


1 16710.00VXR0020

Speci�cation:

Make : ORANGE

Model : Regular- Maxi Vertical

Cat.No : VXR0020









Gasket : Fixed









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780.00

765.00

810.00

880.00

830.00

885.00

930.00

840.00

4800.00

580.00

760.00

1750.00

5600.00

250.00

Set

1

1

1

6/Pack

6/Pack

6/Pack

1

Set

1

1

1

Pair

1

VXR0201 Glass Plate (16x20cm Gel)

VXR0202 20-wells 0.5mm thickness comb

VXR0203 20-wells 1mm thickness comb

VXR0204 20-wells 1.5mm thickness comb

VXR0205 Spacers 0.5mm thickenss

VXR0206 Spacers 1mm thickenss

VXR0207 Spacers 1.5mm thickenss

VXR0208 Gel Caster Maxi

VXR0209 Glass Plate,Comb and Spacers Combo

VXR2010 1mm thickness Acrylic Spacer

VXR2011 1.5mm thickness Acrylic Spacer

VXR2012 Gel Staininig Tray Maxi

VXR2013 Platinum Electrode for Maxi Vertical (Upper+Lower Tank)


Accessories for Maxi Vertical (SDS - PAGE)

Maxi Broad Vertical Gel Electrophoresis Unit (SDS - PAGE Apparatus)


1 21720.00VBR0080

Speci�cation:

Make : ORANGE

Model : Regular- Maxi Broad Vertical

Cat.No : VBR0080

Dimension : 55(L)x20(B)x24(H)





Number of Combs : 80-wells 1mm thickness Sharktooth comb (Well Width-3mm,Interspace-2.1mm) (1 No.)

80-wells 1.5mm thickness Sharktooth comb (Well Width-3mm,Interspace-2.1mm) (1 No.)

40-wells 1mm thickness Sharktooth comb (Well Width-7mm,Interspace-3mm) (1 No.)

40-wells 1.5mm thickness Sharktooth comb (Well Width-7mm,Interspace-3mm) (1 No.)


Gasket : Fixed

Comb Capacity (5mm Deep Well : 15μl,22μl,35μl,52μl








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1650.00

1490.00

1650.00

1730.00

1050.00

1190.00

1240.00

1490.00

1680.00

1770.00

1780.00

7850.00

780.00

980.00

2240.00

9980.00

250.00

Set

1

1

1

1

1

1

6/Pack

6/Pack

6/Pack

1

Set

1

1

1

Pair

1

VBR0801 Glass Plate (40x21cm Gel)

VBR0802 80-wells 0.5mm thickness Sharktooth comb

VBR0803 80-wells 1mm thickness Sharktooth comb


VBR0805 40-wells 0.5mm thickness comb

VBR0806 40-wells 1mm thickness comb


VBR0808 Spacers 0.5mm thickenss

VBR0809 Spacers 1mm thickenss

VBR8010 Spacers 1.5mm thickenss

VBR8011 Gel Caster Maxi Broad

VBR8012 Glass Plate,Comb and Spacers Combo

VBR8013 1mm thickness Acrylic Spacer

VBR8014 1.5mm thickness Acrylic Spacer

VBR8015 Gel Staininig Tray Maxi Broad

VBR8016 Platinum Electrode for Maxi Vertical (Upper+Lower Tank)


Accessories for Maxi Broad Vertical (SDS - PAGE)

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Blotting ApparatusWet Blotting Apparatus & Accessories

ORANGE offers a complete range of Western or Immuno Blotting is the transfer of separated Proteins in a gel to the surface of a thin support membrane matrix. The proteins are bound and immobilized on the membrane. Southern and northern blotting involves the transfer of separated DNA and RNA, respectively, from a gel to a membrane. To perform a transfer, the gel is layered next to a membrane and placed in a voltage gradient perpendicular to the gel. Negatively charged molecules will migrate out from the gel, move towards the positive electrode, and get deposited on the membrane. We offer all different type of models for different applications like Mini,Mini Dual,Midi,Midi Dual & Customized Wet Blots etc.,

Advantages of Blotting Apparatus1. Easy access of proteins or nucleic acids on a blot for further analysis

2. Faster analysis of proteins on the blots as protein diffusion is minimized

Speci�cationProtwins Mini

Wet BlotProtwins MiniDual Wet Blot

Protwins MiniTrio Wet Blot

Protwins MidiWet Blot

Protwins MidiDual Wet Blot

Protwins MidiTrio Wet Blot

Cat.No


Buffer Volume

No.of Gel

Gel Size

Electrode Material

Cooling Unit

Price (INR)

WBP0001

10.5cmx5cmx12cm

400ml

1

8x7cm

Platinum

No

12450.00

WBP0002

15cmx11.5cmx12cm

650ml

2

8x7cm

Platinum

Yes

14900.00

WBP0003

19cmx11.5cmx12cm

700ml

3

8x7cm

Platinum

Yes

17850.00

WDP0001

18cmx7cmx22cm

850ml

1

16x14cm

Platinum

No

16440.00

WDP0002

17.5cmx15cmx22cm

1100ml

2

16x14cm

Platinum

Yes

20670.00

WDP0003

22cmx15cmx22cm

1400ml

3

16x14cm

Platinum

Yes

23590.00

Selection Guide - Protwins Model Wet Blot Apparatus

Speci�cationRapid MiniWet Blot

Rapid MiniDual Wet Blot

Rapid MiniTrio Wet Blot

Rapid MidiWet Blot

Rapid MidiDual Wet Blot

Rapid MidiTrio Wet Blot

Cat.No


Buffer Volume

No.of Gel

Gel Size

Electrode Material

Cooling Unit

Price (INR)

WBR0001

10.5cmx5cmx12cm

400ml

1

8x7cm

Platinum CoatedTitanium Anode

Stainless-Steel Cathode

No

16670.00

WBR0002

15cmx11.5cmx12cm

650ml

2

8x7cm



Yes

18980.00

WBR0003

19cmx11.5cmx12cm

700ml

3

8x7cm



Yes

21890.00

WDR0001

18cmx7cmx22cm

850ml

1

16x14cm



Yes

22440.00

WDR0002

17.5cmx15cmx22cm

1100ml

2

16x14cm



Yes

26690.00

WDR0003

22cmx15cmx22cm

1400ml

3

16x14cm



Yes

29540.00

Selection Guide - Rapid Model Wet Blot Apparatus

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Protwins Mini Wet Blot Apparatus


1 12450.00WBP0001

Speci�cation:

Make : ORANGE

Model : Protwins Mini Wet Blot

Cat.No : WBP0001

Dimension : 10.5cm(L)x5cm(B)x12cm(H)

Gel Holder Cassette : 1 No.


Gel Size : 8x7 cm(1 Gel)



Fiber Pad : 6 Nos.

Foam Pad : 6 Nos.




Protwins Mini Dual Wet Blot Apparatus


1 14900.00WBP0002

Speci�cation:

Make : ORANGE

Model : Protwins Mini Dual Wet Blot

Cat.No : WBP0002

Dimension : 15cm(L)x11.5cm(B)x12cm(H)

Gel Holder Cassette : 2 Nos.


Gel Size : 8x7 cm (2 Gels)



Fiber Pad : 12 Nos.

Foam Pad : 12 Nos.

Cooling Unit Mini : 1 No.




Protwins Model Wet Blot Apparatus

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Protwins Mini Trio Wet Blot Apparatus


1 17850.00WBP0003

Speci�cation:

Make : ORANGE

Model : Protwins Mini Trio Wet Blot

Cat.No : WBP0003








Fiber Pad : 18 Nos.

Foam Pad : 18 Nos.




Protwins Midi Wet Blot Apparatus


1 16440.00WDP0001

Speci�cation:

Make : ORANGE

Model : Protwins Midi Wet Blot

Cat.No : WDP0001




Gel Size : 16x14 cm (1 Gel)



Fiber Pad : 6 Nos.

Foam Pad : 6 Nos.




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Protwins Midi Dual Wet Blot Apparatus


1 20670.00WDP0002

Speci�cation:

Make : ORANGE

Model : Protwins Midi Dual Wet Blot

Cat.No : WDP0002







Fiber Pad : 12 Nos.

Foam Pad : 12 Nos.





Protwins Midi Trio Wet Blot Apparatus


1 23590.00WDP0003

Speci�cation:

Make : ORANGE

Model : Protwins Midi Trio Wet Blot

Cat.No : WDP0003







Fiber Pad : 18 Nos.

Foam Pad : 18 Nos.





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Rapid Mini Wet Blot Apparatus


1 16670.00WBR0001

Speci�cation:

Make : ORANGE

Model : Rapid Mini Wet Blot

Cat.No : WBR0001




Gel Size : 8x7 cm(1 Gel)


No. of Electrodes : Anode and Cathode (Each One)

Fiber Pad : 6 Nos.

Foam Pad : 6 Nos.




Rapid Mini Dual Wet Blot Apparatus


1 18980.00WBR0002

Speci�cation:

Make : ORANGE

Model : Rapid Mini Dual Wet Blot

Cat.No : WBR0002







Fiber Pad : 12 Nos.

Foam Pad : 12 Nos.





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Rapid Mini Trio Wet Blot Apparatus


1 21890.00WBR0003

Speci�cation:

Make : ORANGE

Model : Rapid Mini Trio Wet Blot

Cat.No : WBR0003








Fiber Pad : 18 Nos.

Foam Pad : 18 Nos.




Rapid Midi Wet Blot Apparatus


1 22440.00WDR0001

Speci�cation:

Make : ORANGE

Model : Rapid Midi Wet Blot

Cat.No : WDR0001




Gel Size : 16x14 cm (1 Gel)



Fiber Pad : 6 Nos.

Foam Pad : 6 Nos.




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Rapid Midi Dual Wet Blot Apparatus


1 26690.00WDR0002

Speci�cation:

Make : ORANGE

Model : Rapid Midi Dual Wet Blot

Cat.No : WDR0002







Fiber Pad : 12 Nos.

Foam Pad : 12 Nos.





Rapid Midi Trio Wet Blot Apparatus


1 29540.00WDR0003

Speci�cation:

Make : ORANGE

Model : Rapid Midi Trio Wet Blot

Cat.No : WDR0003







Fiber Pad : 18 Nos.

Foam Pad : 18 Nos.





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890.00

3600.00

490.00

370.00

790.00

6950.00

1440.00

7150.00

790.00

680.00

1170.00

12980.00

250.00

1

1

6/Pack

6/Pack

1

Set

1

1

6/Pack

6/Pack

1

Set

1

WBP0011 Gel Holder Cassette Mini

WBP0012 Platinum Electrode for Mini Single Side

WBP0013 Fiber Pad Mini

WBP0014 Foam Pad Mini

WBP0015 Cooling Unit Mini

WBR0011 Platinum Coated Titanium Anode,Stainless-Steel Cathode for Mini

WDP0011 Gel Holder Cassette Midi

WDP0012 Platinum Electrode for Midi Positive or Negative

WDP0013 Fiber Pad Midi

WDP0014 Foam Pad Midi

WDP0015 Cooling Unit Midi

WDR0011 Platinum Coated Titanium Anode,Stainless-Steel Cathode for Midi


Accessories for Wet Blotting Apparatus

Regular SemiDry Trans Blot ApparatusRegular Mini SemiDry Trans Blot Apparatus


1 9880.00BMR0011

Speci�cation:

Make : ORANGE

Model : Regular Mini SemiDry Blot

Cat.No : BMR0011


Buffer Volume : 50ml (SemiDry)

Active Transfer Area : 10x10 cm (WxL)

Sample Throughput : One 10 x 10cm mini-gel


No. of Electrodes : Graphite Anode and Graphite Cathode (Each One)

Foam Pad : 6 Nos.



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Regular Midi SemiDry Trans Blot Apparatus


1 15450.00BDR0022

Speci�cation:

Make : ORANGE

Model : Regular Midi SemiDry Blot

Cat.No : BDR0022




Sample Throughput : One 20 x 20cm midi-gel


No. of Electrodes : Graphite Anode and Graphite Cathode (Each One)

Foam Pad : 6 Nos.



Ease Advanced Mini SemiDry Trans Blot Apparatus


1 21800.00SME1100

Speci�cation:

Make : ORANGE

Model : Ease Advanced Mini SemiDry Blot

Cat.No : SME1100






No. of Electrodes : Platinum Coated Titanium Anode and Stainless - Steel Cathode (Each One)

Foam Pad : 6 Nos.



Ease Advanced SemiDry Trans Blot ApparatusORANGE newly introduced Ease Advanced SemiDry Blot System 10 x 10cm and 20 x 20cm are ideal for fast transfer of proteins and nucleic

acids without the need for costly accessories such as gel cassettes and tanks. Corrosion-free platinum-coated titanium and stainless steel plate electrodes, corresponding to the anode and cathode, maximise the active area of transfer so that full electrical contact is made between the gel and the membrane, allowing transfer to be completed within an hour with the minimum of heat dissipation, buffer consumption and mess in the laboratory, normally associated with wet blotting techniques.

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Ease Advanced Midi SemiDry Trans Blot Apparatus


1 37500.00SDE2200

Speci�cation:

Make : ORANGE

Model : Ease Advanced Midi SemiDry Blot

Cat.No : SDE2200






No. of Electrodes : Platinum Coated Titanium Anode and Stainless-Steel Cathode (Each One)

Foam Pad : 6 Nos.




3950.00

7470.00

450.00

740.00

13950.00

4480.00

22460.00

8290.00

250.00

1

1

6/Pack

6/Pack

1

1

1

1

1

BMR1101 Graphite Electrode for Single Side

BDR1102 Graphite Electrode for Single Side

BMR1103 Foam Pad Mini (10x10cm)

BMR1104 Foam Pad Midi (20x20cm)

SME1101 Platinum Coated Titanium Anode for Mini Blot

SME1102 Stainless-Steel Cathode for Mini Blot

SDE2201 Platinum Coated Titanium Anode for Midi Blot

SDE2202 Stainless-Steel Cathode for Midi Blot


Accessories for SemiDry Blot Apparatus

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Mini Southern Blot Apparatus


1 3640.00SBM0013

Speci�cation:

Make : ORANGE

Model : Mini Southern Blot

Cat.No : SBM0013




Sample Throughput : One 13 x 13cm gel

Weight Balance : 1 No.




Midi Southern Blot Apparatus


1 5850.00SBD0020

Speci�cation:

Make : ORANGE

Model : Midi Southern Blot

Cat.No : SBD0020




Sample Throughput : One 20 x 20cm gel

Weight Balance : 1 No.




Southern Blot ApparatusSouthern Blot method for the transfer of DNA restriction fragments from agarose gels to cellulose nitrate (nitrocellulose) membranes and

then hybridization of the resulting blot-immobilized DNA to radioactively labeled DNA probes. Since then, the term "Southern blot" has been used to describe any hybridization of DNA on blots to DNA probes, regardless of the type of membrane used.

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Mini Immuno Gel Electrophoresis Unit


1 3490.00IEM0002

Speci�cation:

Make : ORANGE

Model : Mini Immuno Gel Unit

Cat.No : IEM0002



No.of Slides : 2 (7.5cmx2.5cm)

Templates : Counter Current Template (1 No.)

Rocket Template (1 No.)

Immuno Electrophoresis Template (1 No.)

Gel Punch : Single Well

Gel Cutting Tool : Trough Cutter (1 No.)





Immuno ElectrophoresisImmuno Gel Electrophoresis Unit & Accessories

Immuno Gel Electrophoresis Unit

Immunoelectrophoresis is a general name for a number of biochemical methods for separation and characterization of proteins based on electrophoresis and reaction with antibodies. All variants of immunoelectrophoresis require immunoglobulins, also known as antibodies reacting with the proteins to be separated or characterized.

Rocket immunoelectrophoresis (also referred to as electroimmunoassay) is a simple, quick, and reproducible method for determining the concentration of a speci�c protein in a protein mixture. The method, originally introduced by Laurell involves a comparison of the sample of unknown concentration with a series of dilutions of a known concentration of the protein, and requires a monospeci�c antiserum against the protein under investigation. The samples to be compared are loaded side-by-side in small circular wells along the edge of an agarose gel that contains the monospeci�c antibody. These samples (antigen) are then electrophoresed into the agarose gel where interaction between antigen and antibody takes place. In the presence of excess antigen,antibody complex is soluble, but as the antigen moves further into the gel, more antigen combines with antibody until a point of equivalence is reached. At this stage the antigen,antibody complex is insoluble. The end result is a precipitation 'rocket' spreading out from the loading well.

Counter Current Immunoelectrophoresis is a modi�cation of immunoelectrophoresis in which antigen and antibody move in opposite directions and form precipitates in the area between the cells where they meet in concentrations of optimal proportions.

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Midi Immuno Gel Electrophoresis Unit


1 6170.00IED0005

Speci�cation:

Make : ORANGE

Model : Midi Immuno Gel Unit

Cat.No : IED0005



No.of Slides : 5 (7.5cmx2.5cm)

Templates : Counter Current Template (1 No.)

Rocket Template (1 No.)

Immuno Electrophoresis Template (1 No.)

Gel Punch : Single Well

Gel Cutting Tool : Trough Cutter (1 No.)






210.00

380.00

330.00

390.00

480.00

340.00

340.00

340.00

340.00

340.00

1150.00

850.00

1690.00

250.00

1

1

1

10/Pack

10/Pack

2/Pack

2/Pack

2/Pack

2/Pack

2/Pack

1

1

1

1

IEM0201 Single Well Gel Punch

IEM0202 Seven Well Gel Punch

IEM0203 Trough Cutter

IEM0204 Glass Slides (4x4cm 2mm Thickness)

IEM0205 Glass Slides (7.5x2.5cm 2mm Thickness)

IEM0206 Rocket Templates

IEM0207 SRID Templates

IEM0208 Counter Current Templates

IEM0209 Immuno Electrophoresis Templates

IEM0210 ODD Pattern Templates

IEM0211 Levelling Table (25x15cm)

IEM02012 Platinum Electrode for Mini Immuno Single Side

IEM02013 Platinum Electrode for Midi Immuno Single Side


Accessories for Immuno Gel Electrophoresis

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Mini Vertical Paper Gel Electrophoresis Unit


1 6640.00VPM0002

Speci�cation:

Make : ORANGE

Model : Mini Vertical Paper Gel Unit

Cat.No : VPM0002



No.of Strips : 2 Nos.

Paper Supporting Rod : 2 Nos.






Midi Vertical Paper Gel Electrophoresis Unit


1 8650.00VPD0003

Speci�cation:

Make : ORANGE

Model : Midi Vertical Paper Gel Unit

Cat.No : VPD0003




Paper Supporting Rod : 3 Nos.






Paper Gel Electrophoresis

Paper Gel Electrophoresis Unit

Paper gel electrophoresis, a piece of �lter paper is used as the electrophoretic medium to separate the protein components. This technique simply involves application of a small volume of serum as a narrow line across a �lter paper saturated with buffer. The two ends of the paper are in contact with anode and cathode. Proteins start migrating when the electrical �eld is provided. Migrated proteins are examined after staining.

This is widely used in clinical Medicine to separate serum samples in diagnosis and for college level teaching purpose.

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Horizontal Paper Gel Electrophoresis Unit


1 7590.00HPD0006

Speci�cation:

Make : ORANGE

Model : Horizontal Paper Gel Unit

Cat.No : HPD0006

Dimension : 18.5cm(L)x28cm(B)x6.5cm(H)



Paper Supporting Rod : 1 No.






Mini Tube Gel Unit


1 6990.00TGM0075

Speci�cation:

Make : ORANGE

Model : Mini Tube Gel Unit

Cat.No : TGM0075

Dimension : 20cm(L)x13.5m(B)x17.5cm(H)

Glass Tube : 7.5cm (L)x0.2cm (ID) (6 Nos)

Rubber Grommets : 6 Nos (with Hole)

Rubber Grommets : 5 Nos (without Hole)


Glass Tube Fixing Stand : 1 No.





Tube Gel and 2-D Page Gel Unit & Accessories

Tube Gel Unit

Two-dimensional (2-D) gel electrophoresis is an established technique considered to be the best option for high-resolution pro�ling of low-abundance proteins. The analysis of complex protein samples can be tedious, time-consuming, and expensive. Recent advancements in sample fractionation and 2-D electrophoresis enable researchers to overcome these problems in identifying low-abundance proteins in complex biological samples.

2-D electrophoresis begins with 1-D electrophoresis but then separates the molecules by a second property in a direction 90 degrees from the �rst. In 1-D electrophoresis, proteins (or other molecules) are separated in one dimension, so that all the proteins/molecules will lie along a lane but that the molecules are spread out across a 2-D gel.

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Midi Tube Gel Unit


1 9790.00TGM0014

Speci�cation:

Make : ORANGE

Model : Midi Tube Gel Unit

Cat.No : TGM0014


Glass Tube : 14cm (L)x0.2cm (ID) (6 Nos)









Mini 2-D PAGE Gel Unit


1 13850.00TDM7500

Speci�cation:

Make : ORANGE

Model : Mini 2-D PAGE Gel Unit

Cat.No : TDM7500


Glass Tube : 7.5cm (L)x0.2cm (ID) (6 Nos)




Vertical Gel Apparatus : Mini Model (1 set of Unit with all Accessories)






2-D PAGE Gel Unit

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Midi 2-D PAGE Gel Unit


1 19140.00TDD1400

Speci�cation:

Make : ORANGE

Model : Midi 2-D PAGE Gel Unit

Cat.No : TDD1400


Glass Tube : 14cm (L)x0.2cm (ID) (6 Nos)




Vertical Gel Apparatus : Midi Model (1 set of Unit with all Accessories)







790.00

440.00

490.00

490.00

670.00

4780.00

5950.00

250.00

6/Pack

6/Pack

6/Pack

6/Pack

1

1

1

1

TDD1401 Glass Tube (14cm (L)x0.2cm (ID))

TDM7501 Glass Tube (7.5cm (L)x0.2cm (ID))

TDD1402 Rubber Grommets with Hole

TDD1403 Rubber Grommets without Hole

TDD1404 Glass Tube Fixing Stand

TGM7501 Platinum Electrode for Tube Gel Unit (Upper+Lower Tank)

TDM7505 Platinum Electrode for 2D PAGE Gel Unit (Upper+Lower Tank)


Accessories for Tube Gel and 2-D PAGE Unit

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Programmable Power Supply 300V/100mA

Selection Guide - Programmable Power Supplies


1 19000.00PPS3001

Speci�cation:

Make : ORANGE

Model : Programmable Power Supply

Cat.No : PPS3001

Output range : (programmable) 0–300 V, fully adjustable in 1 V steps , 100 mA, fully adjustable in 1 mA steps

Type of output : Constant voltage or constant current with automatic crossover

Output terminals : 3 pair recessed banana jacks in parallel

Timer : 1 min–999 min , fully adjustable

Start /Stop Function : Yes

Display : LCD

Operating conditions : 0–40°C, 0–90% humidity in the absence of condensation

Safety features : No-load detection, sudden load change detection, overload/short-circuit detection,

Input protection : Fuse on both hot and neutral

Input power (actual) : 220 VAC/50hz





1 24500.00PPS3002

Speci�cation:

Make : ORANGE


Cat.No : PPS3002

Output range : (programmable) 0–300 V, fully adjustable in 1 V steps , 200 mA, fully adjustable in 1 mA steps





Display : LCD







POWER SUPPLIESElectrophoresis Power Supplies

Programmable Power Supplies

Programmable Power SuppliesParameters

PPS3001

300V

100mA

3 Parallel

Programmable

19000.00

PPS3002

300V

200mA

3 Parallel

Programmable

24500.00

PPS5005

500V

500mA

4 Parallel

Programmable

38500.00

Cat.No

Voltage

Current

Outputs

Source

Price in INR

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1 38500.00PPS5005

Speci�cation:

Make : ORANGE


Cat.No : PPS5005

Output range : (programmable) 0–500 V, fully adjustable in 1 V steps

500 mA, fully adjustable in 1 mA steps





Display : LCD







Digital Power Supplies

Selection Guide - Digital Power Supplies

Digital Power SuppliesParameters

DPS1003

300V

100mA

4 Parallel

Digital

16500.00

DPS2003

300V

200mA

4 Parallel

Digital

19000.00

PPS4003

300V

400mA

4 Parallel

Programmable

28000.00

DPS5105

500V

500mA

4 Parallel

Digital

35000.00

Cat.No

Voltage

Current

Outputs

Source

Price in INR

Digital Power Supply 300V/100mA


1 16500.00DPS1003

Speci�cation:

Make : ORANGE

Model : Digital Power Supply

Cat.No : DPS1003

Output range : 10–300 V, fully adjustable in 1 V steps,1-100 mA

fully adjustable in 1 mA steps

Type of output : Constant voltage or constant current




Display : 3-digit LED







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LOG

UE



1 19000.00DPS2003

Speci�cation:

Make : ORANGE


Cat.No : DPS2003

Output range : 10–300 V, fully adjustable in 1 V steps,1-200 mA,















1 28000.00PPS4003

Speci�cation:

Make : ORANGE


Cat.No : PPS4003

Output range : (Programmable) 10–300 V, fully adjustable in

1 V steps,1-400 mA, fully adjustable in 1 mA steps





Display : LCD







37

POW

ER S

UPPL

IES

PR

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UC

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LOG

UE



1 35000.00DPS5105

Speci�cation:

Make : ORANGE


Cat.No : DPS5105

Output range : 10–500 V, fully adjustable in 1 V steps,1-500 mA



Output terminals\ : 4 pair recessed banana jacks in parallel










Selection Guide - Regular Electrophoresis Power Supplies

Regular Electrophoresis Power SupplyParameters

APS2501

50,100,150,200,250

100mA

Single

Fixed Voltage

2950.00

APS2502

50,100,150,200,250

200mA

Dual

Fixed Voltage

4900.00

APS2510

50,100,150,200,250

1000mA

4 Parallel

Fixed Voltage

15000.00

APS3150

3000

150mA

Dual

Variable/Fixed

95000.00

APS3060

60

3000mA

Dual

Variable

16000.00

Cat.No

Voltage

Current

Outputs

Source

Price in INR

Power Supply Basic


1 2950.00APS2501

Speci�cation:

Make : ORANGE

Model : Power Supply Basic

Cat.No : APS2501

Output range : 50,100,150,200,250V/100mA

Type of output : Fixed voltage

Display : LED Indications for Output Fixed Voltages

Output terminals : Pair of recessed banana jacks

Enclosures : Mild Steel with Powder Coated


Control : Knob Control to set the Voltages

Input protection : Fuse controlled




Regular Electrophoresis Power Supplies

38

POW

ER S

UPPL

IES

PR

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LOG

UE

Power Supply Basic Dual


1 4900.00APS2502

Speci�cation:

Make : ORANGE

Model : Power Supply Basic Dual

Cat.No : APS2502

Output range : 50,100,150,200,250V/200mA



Output terminals : 2 Pair of recessed banana jacks in parallel








Power Supply 250V/1000mA


1 15000.00APS2510

Speci�cation:

Make : ORANGE

Model : Power Supply 250V/1000mA

Cat.No : APS2510

Output range : 50,100,150,200,250V/1000mA







Input protection : Fuse Controlled




39

POW

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UPPL

IES

PR

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LOG

UE

High Voltage Power Supply


1 95000.00APS3150

Speci�cation:

Make : ORANGE

Model : High Voltage Power Supply

Cat.No : APS3150

Output range : 3000V/150mA

Type of output : Variable

Display : Separate individual LED displays for Volts & Current




Control : Knob Control to set the Voltages and Current





High Current Power Supply


1 16000.00APS3060

Speci�cation:

Make : ORANGE

Model : High Current Power Supply

Cat.No : APS3060

Output range : 60V/3000mA

Type of output : Variable

Display : Separate individual LED displays for Volts & Current




Control : Knob Control to set the Voltages and Current





40

UV T

RANS

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ATOR

PR

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LOG

UE

Selection Guide - UV Transilluminator

UV TransilluminatorUV Transilluminators & UV Portable Lamps

UV -BLB Basic UV -BLB UV-BLB Research UV -Vista UV-VIZ Basic UV-VIZ UltraParameters

UVG3651

365nm

4

14 X 14 CM

12,000.00

UVG3654

365nm

4

24 X 14 CM

18,500.00

UVG3652

365nm

10

28 X 15 CM

40,000.00

UVG3653

365nm

8

20 X 20 CM

35,000.00

UVG3657

365nm/Visible Light

4/4

20 X14 / 20 X 14 CM

25,000.00

UVG3655

365nm/Visible Light

8/8

20 X 20 / 20 X 20 CM

38,500.00

Cat.No

UV Source

No of Tubes

Viewing Area

Price

UV-BI Wavelength UV VIVID UVC Ultra UV Ultra Dual UV Trio White IlluminatorParameters

UVG3625

254nm/365nm

4/4

20 X 20 CM

46,000.00

UVG3121

312nm

8

20 X 20 CM

69,000.00

UVG2541

254nm

8

20 X 20 CM

42,000.00

UVG3631

365nm/312nm

4/4

20 X 20 CM

73,000.00

UVG5161

254/312/365nm

6/6/6

20 *20/ 20*20/20*20 CM

115,000.00

UVG7001

Visible Light

6

20 X 20 CM

8,000.00

Cat.No

UV Source

No of Tubes

Viewing Area

Price

Overview :

All UV transilluminators are equipped with a UV blocking cover, adjustable to different angles for access to the work surface. All ORANGE transilluminators carry a One year warranty.

Application:

Longwave UV - 365nm :

Bacterial Identi�cation * Specimen Staining * Gel Electrophoresis * Chlorination * Fluorochemistry * Pesticide Analysis * Fluorescence Photography * Titration Processes * TLC * Nucleic Acid Visualization * Genetic Experiments * Virology Sanitation: A�atoxin Detection * Rodent Contamination and Lice Detection * Detection of Food Contamination * Milkstone Inspection * E.coli Water Testing * Culture Fluorescence Medical: Medical Diagnosis * Dermatology * Cosmetology

Midrange UV - 312nm :

Gel Electrophoresis * Gel Viewing * Optical Lab Measurements

Shortwave UV - 254nm :

Fluorochemistry * Mercury Detectors * Optical Alignment * Pesticide Analysis * Polymer Curing * Sterilization * DNA Analysis * Biochemical

WARNING : Transilluminators are powerful sources of ultraviolet radiation which can

cause serious damage to unprotected eyes and skinUV

41

UV T

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ATOR

PR

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LOG

UE

UV Transilluminator BLB Basic


1 12000.00UVG3651

Speci�cation:

Make : ORANGE

Model : UV Transilluminator BLB Basic

Cat.No : UVG3651

UV source : 365nm Wave Length

Viewing area : 14x14cm

UV Tube : 4x8 watts UV Tube

Housing : Anti Corrosion treated with Powder coated Mild Steel

UV Safty Shield : Clear Transparent lid for excellent clarity

Input : 220V AC,50-60HZ

Fuse : 1Amp

Anti Slip rubber foot provided for base



UV Transilluminator BLB


1 18500.00UVG3654

Speci�cation:

Make : ORANGE

Model : UV Transilluminator BLB

Cat.No : UVG3654







Fuse : 1Amp




UV Transilluminator BLB Research


1 40000.00UVG3652

Speci�cation:

Make : ORANGE

Model : UV Transilluminator BLB Research

Cat.No : UVG3652







Fuse : 2.5Amp




UV Transilluminators

42

UV T

RANS

ILLU

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ATOR

PR

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LOG

UE

UV Transilluminator Vista


1 35000.00UVG3653

Speci�cation:

Make : ORANGE

Model : UV Transilluminator Vista

Cat.No : UVG3653







Fuse : 2.5Amp




UV-VIZ Basic Transilluminator


1 25000.00UVG3657

Speci�cation:

Make : ORANGE

Model : UV-VIZ Basic Transilluminator

Cat.No : UVG3657

UV source : 365nm Wave Length & Visible Light

Viewing area : 20x14/20x14cm





Fuse : 2.5Amp




UV-VIZ Ultra Transilluminator


1 38500.00UVG3655

Speci�cation:

Make : ORANGE

Model : UV-VIZ Ultra Transilluminator

Cat.No : UVG3655

UV source : 365nm Wave Length & Visible Light






Fuse : 2.5Amp




43

UV T

RANS

ILLU

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ATOR

PR

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LOG

UE

UV-BI Wavelength Transilluminator


1 46000.00UVG3625

Speci�cation:

Make : ORANGE

Model : UV-BI Wavelength Transilluminator

Cat.No : UVG3625

UV source : 254/365nm


UV Tube : 4x8/4x8 watts UV Tube

Housing : Anti Corrosion treated with Powder coated

Mild Steel



Fuse : 2.5Amp




UV VIVID Transilluminator


1 69000.00UVG3121

Speci�cation:

Make : ORANGE

Model : UV VIVID Transilluminator

Cat.No : UVG3121

UV source : 312nm






Fuse : 1Amp




UV Ultra Transilluminator


1 42000.00UVG2541

Speci�cation:

Make : ORANGE

Model : UV Ultra Transilluminator

Cat.No : UVG2541

UV source : 254nm






Fuse : 2.5Amp




44

UV T

RANS

ILLU

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ATOR

PR

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LOG

UE

UV Ultra Dual Transilluminator


1 73000.00UVG3631

Speci�cation:

Make : ORANGE

Model : UV Ultra Dual Transilluminator

Cat.No : UVG3631

UV source : 365/312nm


UV Tube : 4x8/4x8 watts UV Tube




Fuse : 2.5Amp




UV TRIO Transilluminator


1 115000.00UVG5161

Speci�cation:

Make : ORANGE

Model : UV TRIO Transilluminator

Cat.No : UVG5161

UV source : 254/312/365nm

Viewing area : 20x20cm/ 20 X 20 Cms / 20 X 20Cms

UV Tube : 6x8/6x8/6x8 watts UV Tube




Fuse : 2.5Amp




White illuminator


1 8000.00UVG7001

Speci�cation:

Make : ORANGE

Model : White illuminator

Cat.No : UVG7001

UV source : Visible Light






Fuse : 2.5Amp




45

UV T

RANS

ILLU

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ATOR

PR

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LOG

UE

UVL Portable Lamps

UVL Portable Lamp


1 8500.00PVU365

Speci�cation:

Make : ORANGE

Model : UVL Portable Lamp

Cat.No : PVU365

UV : 365nm Long Wavelength

No.of Tubes : 2x8 watts UV Tube

UV Outlet : 200mmX75mm

UV Control : Push to "ON" Switch or IRS1 ON/OFF Switch

Housing : Thermo Plastic


Fuse : 1Amp



UVS Portable Lamp


1 9500.00PVU254

Speci�cation:

Make : ORANGE

Model : UVS Portable Lamp

Cat.No : PVU254

UV : 254nm Short Wavelength






Fuse : 1Amp



UVM Portable Lamp


1 16000.00PVU312

Speci�cation:

Make : ORANGE

Model : UVM Portable Lamp

Cat.No : PVU312

UV : 312nm Mid Wavelength






Fuse : 1Amp



46

UV T

RANS

ILLU

MIN

ATOR

PR

OD

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ATA

LOG

UE

UVS Ultra Portable Lamp


1 22000.00UVC162

Speci�cation:

Make : ORANGE

Model : UVS Ultra Portable Lamp

Cat.No : UVC162

UV : 254nm Short Wavelength

No.of Tubes : 2x15 watts UV Tube (2 Ft)



Housing : MS Cabinet with Thermal Coating / Thermo Plastic


Fuse : 2.5Amp

Ventillation : Cooling Fan Cum Exhaust



UVHL Portable Lamp


1 6500.00PVUS365

Speci�cation:

Make : ORANGE

Model : UVHL Portable Lamp

Cat.No : PVUS365







Fuse : 1Amp



UVFL Portable Lamp With UV Glass Filter


1 10000.00PFL365

Speci�cation:

Make : ORANGE

Model : UVFL Portable Lamp With UV Glass Filter

Cat.No : PFL365



UV Filter Size : 200mmX75mm




Fuse : 1Amp



47

PCR

WOR

K HO

OD

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Selection Guide - PCR Work Hood

PCR Work Hood


1

1

42500.00

35000.00

WST6671

WST5462

Speci�cation:

Make : ORANGE

Model : PCR Work Hood

• Inactivates nucleic acids in 5 to 30 minutes.

• UV Sterilisation of reagent, pipette tips, pipettes etc.

• Protect the sample from external contamination and the user from biohazardous sample.

• Made of 8mm*acrylic glass, hence suitable for work.

• Door with safety cut-off switches, in order to protect the user from UV radiation.

• Four powerfull, timer controlled UV tubes.

• Combined with special re�ectors for ef�cient irradition of the entire work surface

white light illumination for routine work.

• Very easy to clean.

• Work station with bench tray.

• 4 x 8 Watt 254nm (Germicidal Lamp), 2x8 Watt White Light.

• Input Voltage : 220/240 V, 50Hz, Fuse : 2A.

Other Lab InstrumentsPCR Work Hood

Dimension UV Tubes Visible Light Working Tray Timer for UV PriceCat.No

60X60X70 cm

50X40X60 cm

4x8 Watts

2x8 Watts

2x8 Watts

1x8 Watts

SS Surgical Grade

SS Surgical Grade

999 minutes

999 minutes

42500.00

35000.00

WST6671

WST5462

Overview :

The UV Sterilizing PCR Work Hood creates an ideal environment for preparing PCR and other samples by reducing the chance of contamina-tion. The built-in high intensity UV provides a source for inactivation of DNA between experiments. Additional contamination control is provided by the uniquely coated stainless steel design that maintains antimicrobial ef�cacy. The surface resists growth of destructive bacteria,molds and fungi.

WARNING: The unit includes shortwave UV, which is a powerful source of UV radiation

that will cause damage to unprotected eyes and skin. Before operating any unit,

ensure all personnel in the area are properly protected and that instructions for use of

this equipment are followed.

UV

48

TLC

VIEW

ERP

RO

DU

CT

CAT

ALO

GU

E

TLC Viewer


1 42500.00TLC2536

Speci�cation:

Make : ORANGE

Model : TLC Viewer

Cat. No. : TLC2536

EPI-UV Source : 254nm 8 Watts X 2 Tubes, 365nm 8 Watts X 2 Tubes

EPI-VIS : Fluorecent 8 watts X 2 Tubes

Speed Controller : Knob Control (Analog)

Input Voltage : 230V 50Hz AC Supply

Fuse : 2 Amp



• UV Darkhood Metal Cabinet With Powder Coated

• Nitrile Sheet for Anti Corrosion and to Visualize Strips

Overview :

How UV Inspection UV Cabinet works

The front of the box is closed with a door with proper alignment , which can be easy for users to use as required for inserting or marking objects. A glass �lter in the viewing window protects the eyes against re�ected short-wave UV light.

Great care has been taken to ensure the correct distances between UV lamp, object and the observer’s eye in the interest of good illumina-tion and untroubled viewing.

Important Notice :

Hardly any TLC laboratory can be without the use of UV light for inspecting chromatograms. Two types of ultra violet light are required for inspecting Thin-Layer chromatogram

Long-wave UV light 365 nm :

Under long-wave UV light substances that can be excited to �uoresence appear as bright spots, often differently colored, on a dark background. The sensitivity of this detection method increases with the intensity of the UV light and also as more visible light is eliminated.

Short-wave UV light 254nm :

Under 254 nm UV substances absorbing at that wavelength become visible, provided the TLC layer contains a �uorescent indicator, e.g. F254. These substances appear as dark spots on a bright background.

Key features :

• Inspection of Thin-Layer chromatograms and other objects in an undarkened room.

• A glass �lter in the viewing window protects the eyes against re�ected short-wave UV light.

• Made from shock-resistant plastic.

TLC Viewer

49

GEL

ROCK

ERS

PR

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UC

T C

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LOG

UE

Overview :

A rocker is a device used in laboratories for molecular and biological mixing applications.Rockers are commonly used for staining and de-staining gels after electrophoresis, hybridization, washing, blotting, Cell culture and gentle mixing.

Typical features of laboratory rockers include variable speeds. Rockers are often designed to accept stackable platforms or may be out�tted with multiple tiers to increase capacity without increasing the footprint.Platform surfaces are usually covered with rubber pads to prevent objects from slipping during operation.

Gel Rockers

Gel Rocker Without Timer


1 19950.00GRN2525

Speci�cation:

Make : ORANGE

Model : Gel Rocker without Timer

Cat. No. : GRN2525

Motor : DC

Maximum RPM : 60 RPM


Platform Size : 25X25cm

Weight : 4 kg (Approx.)

No. of Platform : Single

Rocking Method : See Saw


Fuse : 1 Amp



Gel Rocker With Electronic Timer


1 23750.00GRT2501

Speci�cation:

Make : ORANGE

Model : Gel Rocker with Electronic Timer

Cat. No. : GRT2501

Motor : DC


Timer : Digital

Timer Range : 0-999 minutes




No. of Platform : Single



Fuse : 1 Amp



ORANGEwe reach more

TM

50

TABL

E TO

P pH

MET

ERP

RO

DU

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CAT

ALO

GU

E

Overview :

A pH meter is an electronic device used for measuring the pH which is either the concentration of Hydrogen ions in an aqueous solution or the activity of the Hydrogen ions in an aqueous solution. The pH will indicate if the solution is acidic or basic, but is not a measure of acidity or alkalinity. pH meters work in liquids though special probes are sometimes used to measure the pH of semi-solid substances. A typical pH meter consists of a special measuring probe (a glass electrode) connected to an electronic meter that measures and displays the pH reading

Table Top pH Meter

Dual Tier Gel Rocker


1 27950.00DTG2502

Speci�cation:

Make : ORANGE

Model : Dual Tier Gel Rocker

Cat. No. : DTG2502

Motor : DC


Timer : Digital

Timer Range : 0-999 minutes




No. of Platform : Dual



Fuse : 1 Amp



Table Top pH Meter


1 7200.00TTP1401

Speci�cation:

Make : ORANGE

Model : Table Top pH Meter

Cat. No. : TTP1401

Range : 0 to 14 pH

Resolution : 0.01pH

Temperature : 0 to 150°C

Electrode : Combined Electrode with BNC Attachment

Input Connection : BNC Socket

Accuracy : ± 0.01 pH ± 1 digit

Display : 3 Digit 0.5” Seven Segment LED Display

Accessories : Electrode stand set, Electrode clip


Fuse : 1 Amp

Weight : 1.7 kg (Approx.)



51

COLO

NY C

OUNT

ERP

RO

DU

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CAT

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GU

E

Overview :

The most of the time-consuming part of carrying out a microbial count is the actual counting process on petri dishes. Colony counters facilitate this task and as a result are essential in every bacteriological laboratory. Their particular advantages are the easy, rapid and reliable counting of bacterial colonies with very simple handling.ORANGE colony counter is easy to use and allows fatigue-free and safe workthanks to its problem-free counting mechanism: Marking the colonies on the petri dish with the marker pen activates the counter.objects from slipping during operation.

Colony Counter

Colony Counter


1 9500.00TCC1499

Speci�cation:

Make : ORANGE

Model : Colony Counter

Cat. No. :TCC1499

Range : 0 – 9999 counts

Resolution : 1 count

Disk Size : 110 mm

Magni�cation : 1.7X

Count Reset : Provided on Front Panel

Operating Environment : 0 – 50ºC / 5 to 95 % RH Non Condensing

Accessories : Magni�er, Electronic Marker Pen Magni�er stand set


Fuse : 1 Amp

Weight : 2.5 kg (Approx.)



52

A gel doc, also known as a gel documentation system, gel image system or gel imager, is equipment widely used in molecular biology laboratories for

the imaging and documentation of nucleic acid and protein suspended within polyacrylamide or agarose gels. These gels are typically stained with

ethidium bromide or other �uorophores such as SYBR Green. Generally, a gel doc includes anultraviolet (UV) light transilluminator, a hood or a darkroom

to shield external light sources and protect the user from UV exposure, and a CCD camera for image capturing.

GEL DOC SYSTEM

Speci�cation:

Hood

• Hood consist of UV Transilluminator ,white light ,Epi UV and

Epi Fluorescent light

• UV Cut off when door is open

• Dark chamber

• 8W re�ectant light

• Door safety switch

• Zoom Lens 8-48mm/f1.0

• Etbr �lter

• Lens dark box

• Built in with 5MP Camera

• Resolution H: 2,592 pixel, V: 1,944 pixel

• Pixel size H: 2.2 µm, V: 2.2 µm

• Lens mount C/CS INTERFACE (ELECTRICAL)

• Interface USB 3.0

• Supply voltage 4.5 VDC to 5.5 VDC

• Current consumption approx 250 mA at 5 VDC INTERFACE (MECHANICAL)

• ADJUSTMENTS (MANUAL) Shutter / s to / s Gain 0 dB to 12 dB

(AUTOMATIC) Shutters to / s Gain 0 dB to 12 dB

• ENVIRONMENTAL Temperature (operation) -5°C to 45°C

1D Gel Analysis Software

• Import common image types up to 48bit, 16 Bit Grey levels

• Interface to control scanner and cameras

• Image pre-processing, rotate, crop, scale, image �lters

• Automatic lane and band detection

• Manual lane and band detection

• Manual correction for lanes and bands

• 6 methods for background noise reduction (automatic and manual)

• Rf correction to correct distorted gels

• MW Calibration using MW standards, Standard editor

• Quanti�cation for bands and lanes

• Normalisation using reference bands detailed list of 5 analysis values

• Reporting, create reports using templates, Export and Print

• Data export to MS Excel, Open Of�ce

• Documentation of gels and export Update via Internet or CD

CONT

ENTS

DNA & RNA Extraction By Spin Coloumn Method Pro NuEx-Ultrapure DNA Extraction from Soil sample 54

Pro NuEx-Ultrapure DNA Gel Extraction Kit 54

Pro NuEx-Ultrapure Genomic DNA Extraction from Bacteria 54

Pro NuEx-Ultrapure Genomic DNA Extraction from Blood 55

Pro NuEx-Ultrapure Genomic DNA Extraction from Bone 55

Pro NuEx-Ultrapure Genomic DNA Extraction from Frozen Tissue 55

Pro NuEx-Ultrapure Genomic DNA Extraction from Hair 56

Pro NuEx-Ultrapure Genomic DNA Extraction from Nail 56

Pro NuEx-Ultrapure Genomic DNA Extraction from Plant 56

Pro NuEx-Ultrapure Genomic DNA Extraction from Plant (CTAB Method) 57

Pro NuEx-Ultrapure Genomic DNA Extraction from Tissue 57

Pro NuEx-mRNA Puri�cation Kit 57

Pro NuEx-PCR Cleanup Extraction Kit 58

Pro NuEx-PCR Products Puri�cation Kit 58

Pro NuEx-Ultrapure Plasmid DNA Extraction Kit 58

Pro NuEx-Total RNA extraction Kit 59

DNA & RNA Extraction By Reagent Pro NuEx-Bacterial Cell Lysis Reagent Kit 59

Pro NuEx-Bacterial Genomic DNA Extraction Kit (Quick one Step Method) 59

Pro NuEx-Bacterial Protein Extraction Kit 59

Pro NuEx-Mammalian Protein Extraction Kit 60

Pro NuEx-Plant DNA Extraction Kit (For PCR Ampli�cation) 60

Pro NuEx-Single Step Ultra-competetent cell preparation Kit 60

Pro NuEx-Total RNA Extraction Kit ( By Trizol) 60

Pro NuEx-Whole Blood Genomic DNA Extraction Kit (From Fresh and Frozen Blood) 60

EXTRACTION KITS

54

EXTR

ACTI

ON K

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DNA & RNA Extraction By Spin Column Method

DNA Extraction Kit provides all the reagents necessary to recover PCR ready DNA from a variety of environmental samples. The kit utilizes a hot detergent lysis process.

Cat.No Product Extraction Price (`)

Pro NuEx-Ultrapure DNA Extraction from Soil Sample 50 Ext

100 Ext

P.O.R*

P.O.R*

OP-3001-50Ex

OP-3001-100Ex Pro NuEx-Ultrapure DNA Extraction from Soil Sample

Components 50 Extraction 100 Extraction

Digestion SolutionElution BufferSoil Lysis SolutionProteinase KSolution ASpin Column & Collection TubePellet Wash SolutionProtocol

50ml5ml

100ml150µl50ml

50 Nos12mlOne

100ml10ml

200ml300µl100ml

100 Nos22mlOne

Pro NuEx-Ultrapure DNA Extraction from Soil Sample

The kit provide a simple and ef�cient method for DNA extraction from agarose gel. The sample is mixed with Binding Buffer and in case of a cut-out gel band, it is heated to dissolve the agarose. In the presence of chaotropic salt, the DNA is bound to the silica membrane of Spin column. Contaminations are removed by simple washing steps with ethanolic Wash Buffer. Finally, the pure DNA is eluted under low salt conditions with slightly alkaline Elution Buffer (2 mMTris/HCl, pH 8.5)


Pro NuEx-Ultrapure DNA Gel Extraction Kit 50 Ext

100 Ext

P.O.R*

P.O.R*

OP-3002-50Ex

OP-3002-100Ex Pro NuEx-Ultrapure DNA Gel Extraction Kit


Binding BufferElution bufferSpin Column & Collection TubeWash BufferProtocol

30ml5ml

50 Nos12mlOne

60ml10ml

100 Nos24mlOne

Excise gel slice withDNA fragment

Lyse Bind Prewash Wash x 2 Elute

Pro NuEx-Ultrapure DNA Gel Extraction Kit

Genomic DNA Extraction Kit (for Bacteria) is spin type product to extract almost all of Gram (-)/(+) Bacteria. Simple & Fast: It takes only 30 ~ 40 minutes to extract genomic DNA Spin Column type: Extract genomic DNA without organic extraction or ethanol precipitation. High purity & yield of genomic DNA can be up to 10µg.


Pro NuEx-Ultrapure Genomic DNA Extraction from Bacteria 50 Ext

100 Ext

P.O.R*

P.O.R*

OP-3003-50Ex

OP-3003-100Ex Pro NuEx-Ultrapure Genomic DNA Extraction from Bacteria


Digestion SolutionElution bufferProteinase KWash BufferSpin Column & Collection TubeProtocol

20ml5ml

150µl12ml

50 NosOne

40ml10ml300µl24ml

100 NosOne

Pro NuEx-Ultrapure Genomic DNA Extraction from Bacteria

Soil Lyse Bind Wash Elute

Pure DNA

Bacteria Lyse Bind Wash Elute

Pure DNA

*Price on Request

55

EXTR

ACTI

ON K

ITS

PR

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The kit used for fast extraction and puri�cation of genomic DNA from clinical samples such as fresh or frozen whole blood,serum and other body �uids. 300µl of sample per spin column is enough to obtain up to 10µg of pure genomic DNA.


Pro NuEx-Ultrapure Genomic DNA Extraction from Blood 50 Ext

100 Ext

P.O.R*

P.O.R*

OP-3004-50Ex

OP-3004-100Ex Pro NuEx-Ultrapure Genomic DNA Extraction from Blood


B1 BufferB2 BufferElution BufferProteinase KTE BufferWash SolutionSpin Column & Collection TubeProtocol

120ml25ml5ml

150µl15ml12ml

50 NosOne

2x120ml50ml10ml300µl30ml14ml

100 NosOne

Pro NuEx-Ultrapure Genomic DNA Extraction from Blood

The kit is designed for fast extraction and puri�cation of DNA from bone. The spin column provided have the ability to bind up to 5-10µg of DNA only.


Pro NuEx-Ultrapure Genomic DNA Extraction from Bone 50 Ext

100 Ext

P.O.R*

P.O.R*

OP-3005-50Ex

OP-3005-100Ex Pro NuEx-Ultrapure Genomic DNA Extraction from Bone


Binding BufferElution BufferExtraction BufferProteinase KWash BufferSpin Column & Collection TubeProtocol

15ml5ml

40ml250µl

12.5ml50 Nos

One

30ml10ml80ml500µl25ml

100 NosOne

Pro NuEx-Ultrapure Genomic DNA Extraction from Bone

The kit is designed for fast extraction and puri�cation of DNA from tissue sample. The kit is provided with spin column can bind up to 10µg of puri�ed DNA. Other impurities like proteins,salts can not bind to the column.


Pro NuEx-Ultrapure Genomic DNA Extraction from Frozen Tissue 50 Ext

100 Ext

P.O.R*

P.O.R*

OP-3006-50Ex

OP-3006-100Ex Pro NuEx-Ultrapure Genomic DNA Extraction from Frozen Tissue


AB solutionATL SolutionElution BufferPBSProteinase Kwash SolutionSpin Column & Collection TubeProtocol

20ml20ml5ml

20ml1ml

12ml50 Nos

One

40ml40ml10ml40ml2ml

24ml100 Nos

One

Pro NuEx-Ultrapure Genomic DNA Extraction from Frozen Tissue

Blood Lyse Bind Wash Elute

Pure DNA

Bone Lyse Bind Wash Elute

Pure DNA

Tissue Lyse Bind Wash Elute

Pure DNA

*Price on Request

56

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Minimum quantity of hair sample is enough to perform the extraction. The kit is designed for fast extraction and puri�cation of DNA from hair sample. The kit is provided with spin column can bind up to 10µg of puri�ed DNA. Other impurities like proteins,salts can not bind to the column.


Pro NuEx-Ultrapure Genomic DNA Extraction from Hair 50 Ext

100 Ext

P.O.R*

P.O.R*

OP-3007-50Ex

OP-3007-100Ex Pro NuEx-Ultrapure Genomic DNA Extraction from Hair


Binding BufferDTTElution BufferExtraction BufferProteinase KWash BufferSpin Column & Collection TubeProtocol

12.5ml500µl5ml

10ml150µl

12.5ml50 Nos

One

25ml1ml

10ml20ml300µl25ml

100 NosOne

Pro NuEx-Ultrapure Genomic DNA Extraction from Hair

The kit is designed for fast extraction and puri�cation of DNA from Nail sample. The kit is provided with spin column can bind up to 10µg of puri�ed DNA.


Pro NuEx-Ultrapure Genomic DNA Extraction from Nail 50 Ext

100 Ext

P.O.R*

P.O.R*

OP-3008-50Ex

OP-3008-100Ex Pro NuEx-Ultrapure Genomic DNA Extraction from Nail


Binding BufferDTTElution BufferExtraction BufferProteinase KWash BufferSpin Column & Collection TubeProtocol

12.5ml500µl5ml

10ml150µl

12.5ml50 Nos

One

25ml1ml

10ml20ml300µl25ml

100 NosOne

Pro NuEx-Ultrapure Genomic DNA Extraction from Nail

The kit is designed for fast extraction and puri�cation of DNA from plant tissue sample. The kit is provided with spin column can bind up to 10µg of puri�ed DNA. Other impurities like proteins,salts can not bind to the column.


Pro NuEx-Ultrapure Genomic DNA Extraction from Plant 50 Ext

100 Ext

P.O.R*

P.O.R*

OP-3009-50Ex

OP-3009-100Ex Pro NuEx-Ultrapure Genomic DNA Extraction from Plant


Elution BufferRNase ASolution A1Solution A2Solution A3Wash SolutionSpin Column & Collection TubeProtocol

5ml150µl15ml2ml

20ml12ml

50 NosOne

10ml300µl30ml4ml

40ml24ml

100 NosOne

Pro NuEx-Ultrapure Genomic DNA Extraction from Plant

Hair Lyse Bind Wash Elute

Pure DNA

Nail Lyse Bind Wash Elute

Pure DNA

Plant Lyse Bind Wash Elute

Pure DNA

*Price on Request

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Homogenised plant tissue is suspended in genomic DNA extraction buffer containing CTAB. Cells are lysed by osmotic shock and cell debris is precipitated and removed by using phenol chloroform and followed by ethanol precipitation of DNA.


Pro NuEx-Ultrapure Genomic DNA Extraction from Plant (CTAB Method) 10 Ext

50 Ext

P.O.R*

P.O.R*

OP-3010-10Ex

OP-3010-50Ex Pro NuEx-Ultrapure Genomic DNA Extraction from Plant (CTAB Method)


Solution ISolution IISolution IIIPrecipitation SolutionProteinase KRNase ATE BufferProtocol

5ml5ml

10ml5ml50µl50µl1mlOne

25ml25ml50ml25ml250µl250µl5mlOne

Pro NuEx-Ultrapure Genomic DNA Extraction from Plant (CTAB Method)

Plant Lyse Bind Wash Elute

Pure DNA

The kit is designed for fast extraction and puri�cation of DNA from tissue sample. The kit is provided with spin column can bind up to 10µg of puri�ed DNA.


Pro NuEx-Ultrapure Genomic DNA Extraction from Tissue 50 Ext

100 Ext

P.O.R*

P.O.R*

OP-3011-50Ex

OP-3011-100Ex Pro NuEx-Ultrapure Genomic DNA Extraction from Tissue


ACL SolutionAB solutionPBSProteinase KElution Bufferwash SolutionSpin Column & Collection TubeProtocol

20ml20ml20ml1ml5ml

12ml50 Nos

One

40ml40ml40ml2ml

10ml24ml

100 NosOne

Pro NuEx-Ultrapure Genomic DNA Extraction from Tissue

Tissue Lyse Bind Wash Elute

Pure DNA

mRNA Isolation Kit is designed to isolate intact poly(A)+ RNA from cells and tissue without requiring phenol or other organic solvents. the kit contain guanidine-thiocyanate based buffers for ef�cient cell and tissue lysis,protein denaturation and Rnase inactivation to keep the mRNA intact. The technology is based on the coupling of Oligo d(T)25 to 1 μm paramagnetic beads which is then used as the solid support for the direct binding of poly(A)+ RNA.

Cat.No Product Preparation Price (`)

Pro NuEx-mRNA Puri�cation Kit 10 Prep

20 Prep

P.O.R*

P.O.R*

OP-3012-10Pr

OP-3012-20Pr Pro NuEx-mRNA Puri�cation Kit

Components 10 Preparation 20 Preparation

Digestion SolutionOligo dT CelluloseR-Elution BufferRNA binding bufferSodium acetate pH4Spin Column & Collection TubeProtocol

10ml10X0.5mg

15ml15ml2ml

10 NosOne

20ml20X0.5mg

30ml30ml4ml

20 NosOne

Pro NuEx-mRNA Puri�cation Kit

Lyse, Homogenizeand Dilute

Cells andTissues

HybridizemRNA to Oligotex

WashmRNA:Oligotex

Complexes

Elute mRNAfrom Oligotex

CollectmRNA:Oligotex

Complexes

mRNA

*Price on Request

58

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The puri�cation method used in these protocols does not require use of phenol, chloroform,or CsCl. The DNA is puri�ed without an additional step of ethanol precipitation.


Pro NuEx-PCR Products Puri�cation Kit 50 Prep

100 Prep

P.O.R*

P.O.R*

OP-3014-50Pr

OP-3014-100Pr Pro NuEx-PCR Products Puri�cation Kit


Binding Buffer IElution BufferWash SolutionSpin Column & Collection TubeProtocol

20ml5ml

12ml50 Nos

One

40ml10ml24ml

100 NosOne

PCR ReactionProduct

Bind Wash 1 Wash 2 ElutionPure DNAfragment

Pro NuEx-PCR Products Puri�cation Kit

The Spin Column Kits provide a simple and ef�cient method for extraction of DNA from agarose gels, and puri�cation of DNA from enzymatic reactions such as PCR or restriction enzyme digestions. The DNA is selectively adsorbed in silica gel-based column and other components are washed away. The DNA is then eluted off the column and can be used for any downstream applications.


Pro NuEx-PCR Cleanup Extraction Kit 50 Prep

100 Prep

P.O.R*

P.O.R*

OP-3013-50Pr

OP-3013-100Pr Pro NuEx-PCR Cleanup Extraction Kit


Cleanup SolutionElution BufferWash Solution Spin Column & Collection TubeProtocol

20ml5ml

12ml50 Nos

One

40ml10ml24ml

100 NosOne

PCRReaction

Components

Prepare ColumnAdd Solution

And SpinBind Wash Elute Ready-to-use DNA

Pro NuEx-PCR Cleanup Extraction Kit

Plasmid DNA Mini Preparation provides a simple, fast and cost-effective method to purify plasmid DNA without phenol/ chloroform extraction. It is based on binding of DNA to silica-based membranes in a chaotropic salt. An average yield of 1-10 ug plasmid DNA can be expected from 1-3 ml overnight bacterial culture.


Pro NuEx-Ultrapure Plasmid DNA Extraction Kit 50 Prep

100 Prep

P.O.R*

P.O.R*

OP-3015-50Pr

OP-3015-100Pr Pro NuEx-Ultrapure Plasmid DNA Extraction Kit


Elution BufferRNAse ASolution ISolution IISolution IIIWash SolutionSpin Column & Colletion TubeProtocol

5ml120µl6ml

12ml25ml12ml

50 NosOne

10ml240µl12ml24ml50ml24ml

100 NosOne

Pro NuEx-Ultrapure Plasmid DNA Extraction Kit

Cell Harvesting DNA Binding Wash ElutionResuspension

LysisNeutralization

*Price on Request

59

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RNA Extraction System provides an method to purify total RNA from various samples such as cultured cells, tissues, bacteria, and yeast. A simple silica-membrane spin-column method can isolate total RNA without phenol/ chloroform extraction and ethanol precipitation. Total RNA with a molecular size greater than 200 nucleotides.


Pro NuEx-Total RNA Extraction Kit 10 Prep

20 Prep

P.O.R*

P.O.R*

OP-3016-10Pr

OP-3016-20Pr Pro NuEx-Total RNA Extraction Kit


Solution G1Solution G2Solution G3DEPC Treated WaterSpin Column & ColletionTube (DEPC treated)Protocol

7ml6ml

2.5ml0.5ml

10 Nos

One

14ml12ml5ml1ml

20 Nos

One

Lyse Sample Puri�ed Total RNA

Bind Wash Elute

Pro NuEx-Total RNA extraction Kit

Quick one step method enables rapid isolation of Genomic DNA from Bacteria .


Pro NuEx-Bacterial Genomic DNA Extraction Kit 1ml

1ml x 5 Nos

P.O.R*

P.O.R*

OP-3018-1ml

OP-3018-5ml Pro NuEx-Bacterial Genomic DNA Extraction Kit

Pro NuEx-Bacterial Genomic DNA Extraction Kit (Quick one Step Method)

Cell lysis solutions are detergent-based, buffers and reagent sets that have been optimized for particular cell lysis applications. Effective cell lysis and protein extraction for different species of organisms and different cell and tissue types require different buffer formulations.


Pro NuEx-Bacterial Cell lysis reagent Kit 1ml

1ml x 5 Nos

P.O.R*

P.O.R*

OP-3017-1ml

OP-3017-5ml Pro NuEx-Bacterial Cell lysis reagent Kit

Pro NuEx-Bacterial Cell Lysis Reagent Kit

DNA & RNA Extraction By Reagent

Bacterial protein extraction kit is designed for the lysis of bacterial cells for the puri�cation of recombinent type proteins. It is useful for the preparation of high inclusion bodies. This method does not require special equipments such as sonicater or french press.


Pro NuEx-Bacterial Protein Extraction Kit For 1LtrCulture medium

For 5LtrCulture medium

P.O.R*

P.O.R*

OP-3019-1L

OP-3019-5L Pro NuEx-Bacterial Protein Extraction Kit

Pro NuEx-Bacterial Protein Extraction Kit

ComponentsFor 1Ltr

Culture mediumFor 5Ltr

Culture medium

10X cell lysis buffer2X SDS Gel loading bufferDNAse & RNaseLysozymePMSFProtocol

5ml0.2ml

200µl each1ml

0.5mlOne

25ml1ml

1ml each5ml

2.5mlOne

60

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This kit contain SSCS solution, enables research to prepare competent E.coli cells in a single step and to transform cells with out heat-shock treatment.


Pro NuEx-Single Step Ultra-competetent cell preparation Kit 25ml

50ml

P.O.R*

P.O.R*

OP-3022-25ml

OP-3022-50ml Pro NuEx-Single Step Ultra-competetent cell preparation Kit

Components 25ml 50ml

SSCS solutionProtocol

25mlOne

50mlOne

Pro NuEx-Single Step Ultra-competetent cell preparation Kit

This kit is designed for quick one step method for DNA extraction from plant leaf. This isolated DNA can be directly used to perform PCR.


Pro NuEx-Plant DNA Extraction Kit (For PCR Ampli�cation) 100 Ext P.O.R*OP-3021-100 Ex

Components 100 Extraction

DNA extraction Solution Protocol

100mlOne

Pro NuEx-Plant DNA Extraction Kit (For PCR Ampli�cation)

It is a proprietary detergent solution designed for ef�cient whole-cell protein extraction from cultured mammalian cells. It enables ef�cient and rapid cell lysis and solubilization of proteins for both suspension and adherent cells. Lysates can be used in many downstream applications.


Pro NuEx-Mammalian Protein Extraction Kit 10 Prep P.O.R*OP-3020-10 Pr

Pro NuEx-Mammalian Protein Extraction Kit

Components 10 Preparation

Extraction BufferPMSFProtease inhibitorProtocol

100ml0.5ml0.5mlOne

This kit is used for rapid isolation of genomic DNA from fresh and frozen blood sample.


Pro NuEx-Whole Blood Genomic DNA Extraction Kit (From Fresh and Frozen Blood) 50 Ext P.O.R*OP-3024-50Ex

Pro NuEx-Whole Blood Genomic DNA Extraction Kit (From Fresh and Frozen Blood)

Components 50 Extraction

Cell Lysis BufferDNA extraction BufferEthanolTE BufferProtocol

125ml15ml30ml1mlOne

Blood Lyse Bind Wash Elute

Pure DNA

Total RNA Extraction (Trizol) is ready to use reagent for the isolation of total RNA from cells and tissues.


Pro NuEx-Total RNA Extraction Kit ( By Trizol) 25ml

50ml

P.O.R*

P.O.R*

OP-3023-25ml

OP-3023-50ml Pro NuEx-Total RNA Extraction Kit ( By Trizol)

Components 25ml 50ml

Total RNA Extraction (Trizol)Protocol

25mlOne

50mlOne

Pro NuEx-Total RNA Extraction Kit (By Trizol)

LyseSample in

Trizol Reagent

Add Chloroform

Transfer Aqueousphase containing RNA

Add ethanoland mix

Add sample tocolumn

WashElute RNA with

RNase-free water

*Price on Request

Teaching KitsPRODUCT CATALOGUE

62

CONT

ENTS

MOLECULARBIOLOGY

GENOMICS Pro TechEx- Agarose Gel Electrophoresis Teaching Kit 64

Pro TechEx- AMES Teaching Kit 64

Pro TechEx- Bacterial Conjugation Teaching Kit 64

Pro TechEx- Bacterial Gene Expression Teaching Kit 65

Pro TechEx- Chloroplast DNA Isolation Teaching Kit 65

Pro TechEx- Chromosomal DNA Extraction from Bacteria Teaching Kit 6

Pro TechEx- Competent Cell Preparation Teaching Kit 65

Pro TechEx- Denaturing Gel Electrophoresis for DNA Sequencing Teaching Kit 66

Pro TechEx- Determination of DNA by Colorimetric method Teaching Kit 66

Pro TechEx- Determination of Tm Value Teaching Kit 66

Pro TechEx- DNA Estimation Teaching Kit 66

Pro TechEx- DNA Estimation by DPA Method Teaching Kit 67

Pro TechEx- DNA Fingerprinting Teaching Kit (Using AFLP Technique) 67

Pro TechEx- DNA Fingerprinting Teaching Kit (Using RAPD Technique) 67

Pro TechEx- DNA Fingerprinting Teaching Kit (Using RFLP Technique) 67

Pro TechEx- DNA Molecular Weight Determination Teaching Kit 68

Pro TechEx- Gel Extraction Teaching Kit 68

Pro TechEx- Gene Cloning Teaching Kit 68

Pro TechEx- Genomic DNA Extraction from Animal Tissue Teaching Kit 69

Pro TechEx- Genomic DNA Extraction from Animal Tissue (High Salt Method) Teaching Kit 69

Pro TechEx- Genomic DNA Extraction from Bacteria Teaching Kit 69

Pro TechEx- Genomic DNA Extraction from Fungi Teaching Kit 70

Pro TechEx- Genomic DNA Extraction from Plant Teaching Kit 70

Pro TechEx- Genomic DNA Extraction from Plant using CTAB Method Teaching Kit 70

Pro TechEx- Genomic DNA Extraction from Whole Blood Teaching Kit 71

Pro TechEx- Genomic DNA Extraction from Yeast Teaching Kit 71

Pro TechEx- Genomic DNA Pur�cation By Using DNA Binding Membrane Teaching Kit 71

Pro TechEx- GFP Cloning Teaching Kit 71

Pro TechEx- Isolation and Characterization of Petite mutant of Yeast Teaching Kit 72

Pro TechEx- Isolation of Antibiotic Resistant and Auxotrophic Mutant of yeast Teaching Kit 72

Pro TechEx- Isolation and Identi�cation of DNA and RNA Teaching Kit 72

Pro TechEx- Lambda Phage DNA Isolation Teaching Kit 73

Pro TechEx- Ligation Teaching Kit 73

Pro TechEx- Long PCR Teaching Kit 73

Pro TechEx- Mitochondrial DNA Isolation Teaching Kit 74

Pro TechEx- Multiplex PCR Teaching Kit 74

Pro TechEx- Mutation Detection and Analysis (Mutagenesis) Teaching Kit 74

Pro TechEx- Nested PCR Teaching Kit 75

Pro TechEx- Nucleosome Mapping by Micrococcal Nulease (Mnase) Digestion Teaching Kit 75

Pro TechEx- Oligonucleotide Puri�cation Teaching Kit 75

Pro TechEx- Optimization of time of Inducer Concentration on E.coli Recombinant Protein

Expression Teaching Kit 76

Pro TechEx- PCR Ampli�cation of β-Galactosidase Gene Teaching Kit 76

Pro TechEx- PCR Application Teaching Kit 76

Pro TechEx- PCR Teaching Kit 76

Pro TechEx- Phage Titration Teaching Kit 77

Pro TechEx- Plasmid Curing Teaching Kit 77

Pro TechEx- Plasmid DNA Isolation Teaching Kit 77

Pro TechEx- Plasmid Puri�cation by DNA Binding Membrane Teaching Kit 78

Pro TechEx- Protoplast Fusion Teaching Kit 78

Pro TechEx- Rapid Plasmid Screening Teaching Kit 78

Pro TechEx- Reporter Gene Assay Teaching Kit 79

Pro TechEx- Restriction Analysis of Plasmid (pBR322 & pUC18) Teaching Kit 79

Pro TechEx- Restriction Digestion Teaching Kit 79

Pro TechEx- Restriction Mapping Teaching Kit 80

Pro TechEx- RT-PCR Teaching Kit 80

Pro TechEx- Single Nucleotide Polymorphisms (SNP) Teaching Kit 80

Pro TechEx- Site Directed Mutagenesis by PCR Teaching Kit 80

Pro TechEx- Southern Blotting Teaching Kit 81

Pro TechEx- Southern Hybridization Teaching Kit 81

Pro TechEx- Study of DNA Methylation Teaching Kit 81

Pro TechEx- Study of DNA Repair Teaching Kit 82

Pro TechEx- Study of Phyical and Chemical Mutagens on The Survival of E.coli Growth

Teaching Kit 82

Pro TechEx- Transduction Teaching Kit 82

Pro TechEx- Transformation (Blue-White Screening ) Teaching Kit 82

Pro TechEx- Transformation of Yeast Cell Using Lithium Acetate Teaching Kit 83

Pro TechEx- Yeast Plasmid DNA Extraction Teaching Kit 83

PROTEOMICS Pro TechEx- Analysis of Protein Structure Teaching Kit 84

Pro TechEx- Antibody Biotin Conjugation Teaching Kit 84

Pro TechEx- Antibody FITC Conjugation Teaching Kit 84

Pro TechEx- Antibody HRP Conjugation Teaching Kit 85

Pro TechEx- Bacterial Alkaline Phosphatase Activity Teaching Kit 85

Pro TechEx- Cellulose Acetate Electrophoresis Teaching Kit 85

Pro TechEx- Clone Gene Analysis by SDS PAGE Teaching Kit 86

Pro TechEx- Enzyme Isolation from Bacteria Teaching Kit 86

Pro TechEx- Enzyme Kinetics Teaching Kit 86

Pro TechEx- Histone Isolation From Nuclei Teaching Kit 86

Pro TechEx- IgY Puri�cation and analysis by SDS-PAGE Teaching Kit 87

Pro TechEx- Immunohistochemistry Teaching Kit 87

Pro TechEx- Isoelectrofocusing and 2D gel Electrophoresis (Tube gel) Teaching Kit 87

Pro TechEx- NATIVE -PAGE Teaching Kit 88

Pro TechEx- Preparation of Biotinylated Probes by Nick Translation Teaching Kit 88

Pro TechEx- Protein Agarose Gel Electrophoresis Teaching Kit 88

Pro TechEx- Protein Degradation Teaching Kit 88

Pro TechEx- Protein Estimation Teaching Kit 89

Pro TechEx- Protein Folding Teaching Kit 89

Pro TechEx- SDS- PAGE Teaching Kit 89

Pro TechEx- Serum Protein Electrophoresis Teaching Kit 90

Pro TechEx- Silver Staining Teaching Kit 90

Pro TechEx- Study of Calf Intestine Alkaline Phosphatase Activity (Dephosporylation of

DNA)Teaching Kit 90

Pro TechEx- Study of Cellular Fractionation Teaching Kit 91

Pro TechEx- Study of Physical Properties of Protein Teaching Kit 91

Pro TechEx- Study of Protein Fingerprinting Teaching Kit 91

Pro TechEx- Western Blotting Teaching Kit 92

TRANSCRIPTOMICS

Pro TechEx- Estimation of RNA by Orcinol Method Teaching Kit 93

Pro TechEx- Invitro Transcription Teaching Kit 93

Pro TechEx- Northern Blotting Teaching Kit 93

Pro TechEx- Northern Hybridization Teaching Kit 93

Pro TechEx- Plant RNA Isolation Teaching Kit 94

Pro TechEx- Preparation of Poly(A) mRNA From Yeast Teaching Kit 94

Pro TechEx- Total RNA Extraction and RT-PCR Teaching Kit 94

Pro TechEx- Total RNA Extraction from Bacteria Teaching Kit 95

Pro TechEx- Total RNA Extraction From Yeast by Trizol Method Teaching Kit 95

Pro TechEx- Total RNA Extraction From Yeast Teaching Kit 95

IMMUNOLOGY

AGGLUTINATION TECHNIQUES Pro TechEx- ABO Blood Grouping With Rh-Factor Teaching Kit 96

Pro TechEx- ASO Latex Agglutination Teaching Kit 96

Pro TechEx- Complement Fixation Test Teaching Kit 96

Pro TechEx- C-Reactive Protein (CRP) Latex Teaching Kit 97

Pro TechEx- Haemagglutination Teaching Kit 97

Pro TechEx- Latex Agglutination Teaching Kit 97

Pro TechEx- RA (Rheumatoid Arthritis) Test Teaching Kit 97

Pro TechEx- RPR Test Teaching kit 98

Pro TechEx- VDRL Test Teaching Kit 98

Pro TechEx- Widal Slide Test Teaching Kit 98

Pro TechEx- Widal Tube Test Teaching Kit 98

63

CONT

ENTS

CHROMATOGRAPHY TECHNIQUES Pro TechEx- Af�nity Chromatography Teaching Kit 99

Pro TechEx- Column Chromotography Teaching Kit 99

Pro TechEx- Gel Filtration Chromatography Teaching Kit 99

Pro TechEx- IgG Puri�cation Teaching Kit 100

Pro TechEx- Immobolized Metal Ion Af�nity Chromatography Teaching Kit 100

Pro TechEx- Immunoglobulin Puri�cation Teaching Kit 100

Pro TechEx- Ion Exchange Chromatography (Antibody Puri�cation ) Teaching Kit 100

Pro TechEx- Ion Exchange Chromatography (Lysozyme Puri�cation) Teaching Kit 101

Pro TechEx- Paper Chromatography Teaching Kit 101

Pro TechEx- Thin Layer Chromotography Teaching Kit 101

ELISA TECHNIQUES Pro TechEx- Antibody Capture ELISA Teaching Kit 101

Pro TechEx- Antigen Capture ELISA Teaching Kit 102

Pro TechEx- Competitive ELISA Teaching Kit 102

Pro TechEx- Dot ELISA Teaching Kit 102

Pro TechEx- Sandwich ELISA Teaching Kit 102

HAEMATOLOGY Pro TechEx- Analysis of blood components Teaching Kit 103

Pro TechEx- Enumeration Of RBC with Hemocytometer Teaching Kit 103

Pro TechEx- Enumeration Of RBC without Hemocytometer Teaching Kit 103

Pro TechEx- Enumeration Of WBC with Hemocytometer Teaching Kit 104

Pro TechEx- Enumeration Of WBC without Hemocytometer Teaching Kit 104

Pro TechEx- GOD/POD Teaching Kit 104

Pro TechEx- Haemoglobin (Hb) Estimation By Cyanmethaemoglobin Method Teaching Kit 104

Pro TechEx- Isolation of Lymphocytes Teaching Kit 105

Pro TechEx- Isolation of Monocytes Teaching Kit 105

Pro TechEx- Isolation of Rosette Cells Teaching kit 105

Pro TechEx- Pregnancy Test (Card Method)Teaching Kit 105

Pro TechEx- Pregnancy Test (Slide Method)Teaching Kit 106

Pro TechEx- SGOT Teaching Kit 106

Pro TechEx- SGPT Teaching Kit 106

PRECIPITATION TECHNIQUES

Pro TechEx- Counter Current Immunoelectrophoresis Teaching Kit 107

Pro TechEx- Immunoeletrophoresis Teaching Kit 107

Pro TechEx- Immunoprecipitation Teaching Kit 107

Pro TechEx- Ouchterlony Double Immuno Diffusion (Pattern) Teaching Kit 108

Pro TechEx- Ouchterlony Double Immuno Diffusion (Titration) Teaching Kit 108

Pro TechEx- Quantitative Preciptin Assay Teaching Kit 108

Pro TechEx- Rocket Immunoelectrophoresis Teaching Kit 109

Pro TechEx- Single Radial Immunodiffusion Teaching Kit 109

MICROBIOLOGY

ISOLATION & IDENTIFICATION OF MICROBES Pro TechEx- Isolation And Identi�cation Of Antibiotic Producing Microorangism Teaching Kit 110

Pro TechEx- Isolation And Identi�cation Of Enzyme Producing Microorganism Teaching Kit 110

Pro TechEx- Isolation And Identi�cation Of Rhizobium From Root Nodules Teaching Kit 110

Pro TechEx- Isolation And Identi�cation Of Soil Bacteria Teaching Kit 111

Pro TechEx- Isolation And Identi�cation Of Yeast From Curd Or Fruit Juice Teaching Kit 111

Pro TechEx- Isolation Of Molds From Soil Teaching Kit 111

GROWTH KINETICS TECHNIQUES Pro TechEx- Antibiotic Sensitivity Test Teaching Kit 111

Pro TechEx- Bacterial Growth Curve Teaching Kit 112

Pro TechEx- Effect Of Different pH On Bacterial Growth Teaching Kit 112

Pro TechEx- Effect Of Different Salt Concentration On Bacterial Growth Teaching Kit 112

Pro TechEx- Effect Of Different Temperature On Bacterial Growth Teaching Kit 112

STAINING TECHNIQUES Pro TechEx- Acid Fast Staining Teaching Kit 113

Pro TechEx- Capsular Staining Teaching Kit 113

Pro TechEx- Endospore Staining Teaching Kit 113

Pro TechEx- Flagella Staining Teaching Kit 114

Pro TechEx- Gram Staining Teaching Kit 114

Pro TechEx- Lactophenol Cotton Blue Staining Teaching Kit 114

Pro TechEx- Metachromatic Granules Staining Teaching Kit 114

Pro TechEx- Monochrome Staining Teaching Kit 115

Pro TechEx- Negative Staining Teaching Kit 115

Pro TechEx- Simple Staining Method For Bacteria In Milk Teaching Kit 115

Pro TechEx- Simple Staining Teaching Kit 116

TISSUE CULTURE TECHNIQUES Pro TechEx- Plant Tissue Culture Teaching Kit 116

ANIMAL TISSUE CULTURE Pro TechEx- Culture Of HeLa S3 Cells In Suspension ( without CO2 System)

Teaching Kit 116

Pro TechEx- Preparation Of Chicken Embryo Fibroblast (In CO2 Incubator)

Teaching Kit 116

APOPTOSIS

Pro TechEx- MTT Assay Teaching Kit 117

Pro TechEx- Determination Of Apoptosis By Staining Method Teaching Kit 117

64

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MOLECULAR BIOLOGY

Agarose gel electrophoresis is a method used in biochemistry, molecular biology and clinical chemistry to separate a mixed population of DNA or proteins in a matrix of agarose. The proteins may be separated by charge and/or size (IEF agarose, essentially size independent), and the DNA and RNA fragments by length. Biomolecules are separated by applying an electric �eld to move the charged molecules through an agarose matrix, and the biomolecules are separated by size in the agarose gel matrix

Cat.No Product Experiments Price (`)

Pro TechEx -Agarose Gel Electrophoresis Teaching Kit 5 Exps

20 Exps

1700.00

3300.00

OP-3101-5xp

OP-3101-20xp Pro TechEx -Agarose Gel Electrophoresis Teaching Kit


Pro TechEx - Bacterial Conjugation Teaching Kit 5 Exps

15 Exps

3900.00

8000.00

OP-3103-5xp

OP-3103-15xp Pro TechEx - Bacterial Conjugation Teaching Kit

COMPONENTS

Agarose (RT)50X TAE (RT)Ethidium Bromide (RT)Gel Loading buffer (RT)0.5ml tubes (RT)DNA Sample (-20°C)Ladder (-20°C)Protocol

COMPONENTS

Donor Strain A (4°C)Recipient Strain B (4°C)Antibiotic (4°C)Agar (RT)LB Broth (RT)Protocol

Pro TechEx - Agarose Gel Electrophoresis Teaching Kit

A test to determine whether a chemical is a mutagen.This method uses bacteria to test whether a given chemical can cause mutations in the DNA of the test organism. More formally, it is a biological assay to assess the mutagenic potential of chemical compounds.A positive test indicates that the chemical is mutagenic and therefore may act as a carcinogen, because cancer is often linked to mutation. The test serves as a quick and convenient assay to estimate the carcinogenic potential of a compound.


Pro TechEx - AMES Teaching Kit 5 Exps 4500.00OP-3102-5xp

Pro TechEx - AMES Teaching Kit

Bacterial conjugation is the transfer of genetic material (plasmid) between bacterial cells by direct cell-to-cell contact or by a bridge-like connection between two cells.

Pro TechEx - Bacterial Conjugation Teaching Kit

COMPONENTS

K2HPO

4 (4°C)

KH2PO

4 (4°C)

Ammonium Sulphate (RT)Tri-Sodium Citrate (RT)MgSO

4 (4°C)

Agar (RT)

Glucose (RT)E.coli (AB1157) ( 4°C)Arginine (RT)Proline (RT)Leucine (RT)Thrieonine (RT)

Vit. B12

(4°C)LB Broth (RT)Chemical Mutagen ( NH

2OH) (RT)

NaOH Solution 4M (RT)Microfuge Tubes (RT)

GENOMICS

65

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Pro TechEx -Competent Cell Preparation Teaching Kit 10 Exps

20 Exps

4500.00

7800.00

OP-3107-10xp

OP-3107-20xp Pro TechEx -Competent Cell Preparation Teaching Kit

COMPONENTS

STE Buffer (4°C)BSA (4°C)BME (4°C)ST Buffer (4°C)TE Saline Buffer (4°C)Isopropanol (RT)

Potassium acetate (RT)SDS (RT)EDTA (RT)Ethanol I (RT)Ethanol II (RT)Protocol

Tris saturated phenol (4°C)Phenol/Chloroform (4°C)Chloroform /Isoamyl alcohol (4°C)Magnesium acetate (RT)Ammonium acetate (RT)Dnase I (-20°C)

COMPONENTS

E.coli Host (4°C)Solution A (CaCl

2) (4°C)

Agar (RT)LB Broth (RT)

Glycerol (RT)Protocol

Bacterial Gene expression is the process by which information from a gene is used to the synthesis a functional gene product.


Pro TechEx - Bacterial Gene Expression Teaching Kit 5 Exps 5300.00OP-3104-5xp

Pro TechEx - Bacterial Gene Expression Teaching Kit

In all land plants and algae, chloroplast DNA (cpDNA) has been found to exist as a single circular molecules ranging from 80 to 300 kb. The chloroplast genome is densely packed with genes. Chloroplast gene products identi�es function either in photosynthesis or as components of the chloroplast protein synthesizing system.In this procedure, sucrose step gradient procedure is used to isolate chloroplast and cpDNA.


Pro TechEx - Chloroplast DNA Isolation Teaching Kit 10 Exps 9500.00OP-3105-10xp


Pro Tech Ex- Chromosomal DNA Extraction from Bacteria Teaching Kit 10 Exps 3500.00OP-3106-10xp

Pro TechEx -Chloroplast DNA Isolation Teaching Kit

Many different methods and technologies are available for the isolation of chromosomal DNA. In general, all methods involve disruption and lysis of the starting material followed by the removal of proteins and other contaminants and �nally recovery of the DNA. Removal of proteins is typically achieved by digestion with proteinase K, followed by salting-out, organic extraction, or binding of the DNA to a solid-phase support (either anion-exchange or silica technology). DNA is usually recovered by precipitation using ethanol or isopropanol.

Pro Tech Ex- Chromosomal DNA Extraction from Bacteria Teaching Kit

There are several ways to prepare competent cells for plasmid DNA transformation. This is the chemical method(CaCl2). Advantages are that

it’s simple to complete, requires no special equipment and gives good transformation ef�ciencies

Pro TechEx -Competent Cell Preparation Teaching Kit

COMPONENTS

Recombinant bacterialculture (4°C)Inducer(IPTG) (4°C)Ampicillin (4°C)LB broth powder (RT)Separating gel mix (4°C)Stacking gel mix (4°C)

APS (RT)Sample Solubilizing buffer (4°C)Running gel buffer mix (RT)Stainer (RT)Destainer (RT)Protocol

COMPONENTS

E.coli strain (4°C)50X TAE (RT)LB media (RT)Phenol/Chloroform (25:24) (RT)Proteinase K (4°C)SDS (RT)TE Buffer (RT)

3M Sodium acetate pH 5.2 (RT)Agarose (RT)DNA Marker (-20°C)Ethanol (70%) (RT)Ethidium Bromide (RT)Gel loading dye (RT)Isopropanol (100%) (RT)Protocol

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Thin polyacrylamide gels that contain a high concentration of urea as a denaturant are capable of resolving short (<500 nucleotides) single-stranded fragments of DNA or RNA that differ in length by as little as one nucleotide. Such gels are uniquely suited for nucleic acid sequence analysis, which is required, for instance, for all footprinting protocols. Thicker gels are often used to purify oligonucleotides. This appendix describes the pouring, running, and processing of a typical sequencing gel, which is 40 cm long with a uniform thickness of 0.4 mm, containing 7 M urea and 4% to 8% acrylamide.


Pro TechEx - Denaturing Gel Electrophoresis for DNA Sequencing Teaching Kit 5 Exps

10 Exps

4800.00

8000.00

OP-3108-5xp

OP-3108-10xp Pro TechEx - Denaturing Gel Electrophoresis for DNA Sequencing Teaching Kit


Pro TechEx -Determination of Tm Value Teaching Kit 5 Exps

10 Exps

4000.00

6000.00

OP-3110-5xp

OP-3110-10xp Pro TechEx -Determination of Tm Value Teaching Kit


Pro TechEx -DNA Estimation Teaching Kit 10 Exps

20 Exps

3900.00

6000.00

OP-3111-10xp

OP-3111-20xp Pro TechEx -DNA Estimation Teaching Kit


Pro TechEx - Determination of DNA by Colorimetric method Teaching Kit 5 Exps 1500.00OP-3109-5xp

Pro TechEx - Denaturing Gel Electrophoresis for DNA Sequencing Teaching Kit

The method depends on the production of a pink color by the reaction of cysteine and sulfuric acid with DNA. Since pentoses do not yield a color reaction with cysteine and sulfuric acid, yeast nucleic acid is unreactive. Therefore, despite the presence of ribonucleic acid (RNA), a direct estimation of deoxyribonucleic acid can be made.

Pro TechEx - Determination of DNA by Colorimetric method Teaching Kit

Note that ‘melting’ in this sense is not a change of aggregate state, but simply the dissociation of the two molecules of the DNA double helix.If a homogenous solution of identical double-stranded DNA molecules is heated, the strands dissociate increasingly at higher tempera-tures can be interpreted from standard curve.

Pro TechEx - Determination of Tm Value Teaching Kit

Estimation of DNA is based on the Ethidium Bromide Fluorecent Staining of DNA. Ethidium Bromide is a Fluorescent dye, Which intercalates between the stacked bases. The �uorescent yield of the dye - DNA complax is much graeter than the unbound dye. UV irradiation at 254nm is absorbed by the DNA and transmitted to the dye and the bounded dye itself absorbs radiation at 302nm and 366nm

Pro TechEx -DNA Estimation Teaching Kit

COMPONENTS

40 % Acrylamide (4°C)APS (RT)TEMED (4°C)Stacking Buffer (4°C)Separating Buffer (4°C)Urea (RT)Oligonucleotides (-20°C)Sample Solubilizing buffer (RT)

Wash buffer (RT)TE Buffer (RT)Gel loading dye (RT)Ethidium bromide (RT)50X TAE (RT)TLC plate (with Fluorescence indicator) RTProtocol

COMPONENTS

DNA sample-I (-20°C)DNA sample-II (-20°C)DNA sample-III (-20°C)Standard DNA Solution (-20°C)Agarose (RT)

Ethidium Bromide (RT)Gel loading dye (RT)50X TAE buffer (RT)Blank solution (RT)Protocol

COMPONENTS

Cysteine Hcl (4°C)Indicator solution (RT)Crude DNA mix (4°C)Protocol

COMPONENTS

DNA sample I (-20°C)DNA sample II (-20°C)DNA diluent buffer (10X) (RT)Microfuge tubes (RT)Protocol

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When DNA is treated with diphenylamine under the acidic condition a bluish green colored complex is formed which has an absorption peak at 595nm.This reaction is given by 2 -deoxypentose in general. In acidic solution deoxypentose are converted into a highly reactive β- hydroxyl leavulinic aldehyde which reacts with diphenylamine gives bluish green colored complex. The colour intensity was measured using a red �lter at 595nm.

Pro TechEx -DNA Estimation by DPA Method Teaching Kit

Ampli�ed Fragment Length Polymorphisms (AFLPs) are differences in restriction fragment lengths caused by SNPs or INDELs that create or abolish restriction endonuclease recognition sites.The AFLP technique is based on the selective PCR ampli�cation of restriction fragments from a total digest of genomic DNA. After �nal Ampli�cation, selectively ampli�ed fragments are separated by gel electrophoresis and visualized.

Pro TechEx-DNA Finger printing Teaching kit (Using AFLP Technique)

Methods for identifying isolates of various pathogenic bacteria by DNA �ngerprinting with random primers (RAPD) have been described recently. In these methods many primers are screened and the primers that generate the most informative DNA pattern are selected. A new strategy that simpli�es the primer selection process for RAPD �ngerprinting has been developed in our laboratory. In this approach, one or more degenerate nucleotides is introduced into the core RAPD primer sequence at various nucleotide positions. Results show that a single degener-ate nucleotide in the primer sequence can signi�cantly change the DNA pro�le obtained for the same template.

Pro TechEx-DNA Fingerprinting Teaching Kit (Using RAPD Technique)

In molecular biology, restriction fragment length polymorphism, or RFLP (commonly pronounced “rif-lip”), is a technique that exploits variations in homologous DNA sequences. It refers to a difference between samples of homologous DNA molecules that come from differing locations of restriction enzyme sites, and to a related laboratory technique by which these segments can be illustrated. In RFLP analysis, the DNA sample is broken into pieces (digested) by restriction enzymes and the resulting restriction fragments are separated according to their lengths by gel electrophoresis.

Pro TechEx-DNA Fingerprinting Teaching Kit (Using RFLP Technique)

COMPONENTS

Standard DNA (-20°C)Test DNA (-20°C)Diphenylamine solution (RT)Protocol


Pro TechEx - DNA Estimation by DPA Method Teaching Kit 5 Exps

10 Exps

2000.00

3000.00

OP-3112-5xp

OP-3112-10xp Pro TechEx - DNA Estimation by DPA Method Teaching Kit


Pro TechEx-DNA Fingerprinting Teaching Kit (Using RAPD Technique) 5 Exps

20 Exps

5300.00

19000.00

OP-3114-5xp

OP-3114-20xp Pro TechEx-DNA Fingerprinting Teaching Kit (Using RAPD Technique)


Pro TechEx-DNA Finger printing Teaching kit (Using AFLP Technique) 5 Exps 7100.00OP-3113-5xp

COMPONENTS

Agarose (RT)50XTAE (RT)Gel loading dye (RT)Ethidium Bromide (RT)EcoRI (-20°C)Restriction buffer (-20°C)

T4 Ligase (-20°C)Ligation buffer (-20°C)Templet DNA (-20°C)Taq polymerase (-20°C)Taq buffer (-20°C)dNTP 10mM (-20°C)

MgCl2 (RT)

EcoRI Primer (-20°C)EcoRI adaptors (F-R) (-20°C)PstI (-20°C)PstI adaptors (-20°C)Protocol

COMPONENTS

Control DNA I (-20°C)Control DNA II (-20°C)Test DNA (-20°C)10mM dNTP (-20°C)MgCl

2 (-20°C)

Taq BufferTaq polymerase (-20°C)

Random primer (-20°C)1Kb DNA ladder (-20°C)Agarose (RT)Ethidium bromide (RT)Gel loading Dye (RT)50X TAE (RT)Protocol

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Pro TechEx-DNA Fingerprinting Teaching Kit (Using RFLP Technique) 5 Exps

20 Exps

5200.00

18000.00

OP-3115-5xp

OP-3115-20xp Pro TechEx-DNA Fingerprinting Teaching Kit (Using RFLP Technique)


Pro TechEx-DNA Molecular Weight Determination Teaching Kit 10 Exps 5800.00OP-3116-10xp


Pro TechEx-Gel Extraction Teaching Kit 5 Exps

20 Exps

3500.0

7000.00

OP-3117-5xp

OP-3117-20xp Pro TechEx-Gel Extraction Teaching Kit

COMPONENTS

DNA Sample I (-20°C)DNA Sample II (-20°C)Control DNA (-20°C)DNA Ladder (-20°C)Hind III enzyme (-20°C)Enzyme Buffer (-20°C)Sterile water (RT)

Agarose (RT)50X TAE (RT)Gel Loading Dye (RT)Ethidium Bromide (RT)0.2ml tubes (RT)Protocol

Agarose gel electrophoresis method is used to measure the molecular size of DNA and RNA molecules. For separation of very large DNA molecules. The migration rates of DNA molecule depend upon their respective molecular weight. This simple and fast method is frequently used to determine the size of a linear DNA fragment by comparing its mobility to a DNA fragment marker of known size.

Pro TechEx-DNA Molecular Weight Determination Teaching Kit

In molecular biology, gel extraction or gel isolation is a technique used to isolate a desired fragment of intact DNA from an agarose gel follow-ing agarose gel electrophoresis. After extraction, fragments of interest can be mixed, precipitated, and enzymatically ligated together in several simple steps. This process, usually performed on plasmids.After DNA samples are run on an agarose gel, extraction involves four basic steps: identifying the fragments of interest, isolating the corresponding bands, isolating the DNA from those bands, and removing the accompanying salts and stain.

Pro TechEx-Gel Extraction Teaching Kit

Gene cloning is the process in which a gene of interest is located and copied (cloned) out of DNA extracted from an organism. When DNA is extracted from an organism, all of its genes are extracted at one time. This DNA, which contains thousands of different genes.

Pro TechEx-Gene Cloning Teaching Kit

COMPONENTS

50X TAE (RT)DNA sample I (4°C)DNA sample II (4°C)DNA marker (4°C)Agarose (RT)Gel loading Dye (RT)Ethidium Bromide (RT)Protocol

COMPONENTS

50X TAE (RT)Ethidium bromide (RT)Gel loading dye (RT)Silica powder (RT)Solubilizing buffer (RT)TE Buffer (RT)Wash buffer (RT)

DNA sample (-20°)DNA marker (-20°)Agarose (RT)Protocol

Excise gel slice withDNA fragment

Lyse Bind Prewash Wash x 2 Elute

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Pro TechEx-Gene Cloning Teaching Kit 5 Exps 4700.00OP-3118-5xp

COMPONENTS

Transformed culture(Control) (-20°C)T4 DNA Ligase (-20°C)10X Ligase buffer (-20°C)Insert DNA (-20°C)Vector DNA (-20°C)ATP (-20°C)Sterile water (-20°C)

Solution-I (4°C)Ampicillin (4°C)E.coli Host (4°C)LB broth (RT)Agar (RT)1.5 ml Microfuge tubes (RT)Protocol (RT)

COMPONENTS

50X TAE (RT)Agarose (RT)Chloroform (RT)Ethidium Bromide (RT)Gel loading Dye (RT)Guanidine thiocyanate (RT)

70% Ethanol (RT)Isopropanol (RT)RNase A (-20°C)TE buffer (RT)DNA Marker (-20°C)Protocol

COMPONENTS

50X TAE (RT)Agarose (RT)100% Ethanol (RT)Ethidium Bromide (RT)Gel loading Dye (RT)TNES Buffer (RT)

70% Ethanol (RT)Tris EDTA (RT)DNA Marker (-20°C)Protocol

A simple technique for the isolation of very high molecular weight genomic DNA from animal tissues and cells is described. The method involves rapid isolation of nuclei and their embedding in agarose beads followed by extraction of lipids and proteins with SDS. The protocol does not require proteolytic digestion and the whole procedure can be completed in 1 day. The isolated DNA is digestible by restriction enzymes and free of ligase inhibitors.

Pro TechEx-Genomic DNA Extraction From Animal Tissue Teaching Kit

A simple technique for the isolation of very high molecular weight genomic DNA from animal tissues and cells is described. The method involves rapid isolation of nuclei and their embedding in agarose beads followed by extraction of lipids and proteins with SDS. The protocol does not require proteolytic digestion and the whole procedure can be completed in 1 day. The isolated DNA is digestible by restriction enzymes and free of ligase inhibitors.

Pro TechEx-Genomic DNA Extraction From Animal Tissue (High Salt Method) Teaching Kit

Many different methods and technologies are available for the isolation of genomic DNA. In general, all methods involve disruption and lysis of the starting material followed by the removal of proteins and other contaminants and �nally recovery of the DNA. Removal of proteins is typically achieved by digestion with proteinase K, followed by salting-out, organic extraction, or binding of the DNA to a solid-phase support (either anion-exchange or silica technology). DNA is usually recovered by precipitation using ethanol or isopropanol.

Pro TechEx-Genomic DNA Extraction From Bacteria Teaching Kit


Pro TechEx-Genomic DNA Extraction From Animal Tissue Teaching Kit 10 Exps

20 Exps

3200.00

4750.00

OP-3119-10xp

OP-3119-20xp Pro TechEx-Genomic DNA Extraction From Animal Tissue Teaching Kit


Pro TechEx-Genomic DNA Extraction From Animal Tissue (High Salt Method) Teaching Kit 10 Exps

20 Exps

3200.00

4750.00

OP-3120-10xp

OP-3120-20xp Pro TechEx-Genomic DNA Extraction From Animal Tissue (High Salt Method) Teaching Kit


Pro TechEx-Genomic DNA Extraction From Bacteria Teaching Kit 10 Exps

20 Exps

2800.00

4100.00

OP-3121-10xp

OP-3121-20xp Pro TechEx-Genomic DNA Extraction From Bacteria Teaching Kit

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COMPONENTS

E.coli strain (4°C)50X TAE (RT)LB media (RT)Phenol/Chloroform (25:24) (RT)Proteinase K (4°C)SDS (RT)TE Buffer (RT)3M Sodium acetate pH 5.2 (RT)

Agarose (RT)DNA Marker (-20°C)Ethanol (70%) (RT)Ethidium Bromide (RT)Gel loading dye (RT)Isopropanol (100%) (RT)Protocol

COMPONENTS

Extraction buffer (4°C)CTAB (RT)5M NaCl (RT)Choloroform/ Isoamyl alcohol (RT)70% Ethanol (RT)

TE buffer (RT)Rnase (-20°C)Isopropanol (RT)Protocol

COMPONENTS

3M Sodium acetate (RT)70%Ethanol (RT)Chloroform/isoamyl alcohol (24:1) (RT)Extraction buffer (RT)Isopropanol (RT)TE Buffer (RT)β mercapto ethanol (RT)

50X TAE (RT)Agarose (RT)Ethidium Bromide (RT)Gel loading dye (RT)RNase A (-20°C)Protocol

COMPONENTS

Agarose (RT)50X TAE (RT)Ethidium bromide (RT)Gel loading dye (RT)SDS (RT)Potassium acetate (RT)70% Ethanol (RT)

TE Buffer (RT)3M sodium acetate (pH 5.2) (RT)EBA(Extraction buffer A) (4°C)EBB(Extraction buffer B) (4°C)Isopropanol (4°C)DNA Marker (4°C)Protocol

We have developed a new, simple and effective method for extraction of fungal genomic DNA. The initial steps involved suspension of freeze-dried mycelium in buffer containing sodium dodecyl sulphate, detachment of DNA from polysaccharides by mild shearing, NaCl precipi-tation of polysaccharides and protein, chloroform extraction and ethanol precipitation. The ethanol precipitate was then subjected to a second round of mild shearing, NaCl precipitation, chloroform extraction and ethanol precipitation. The procedure required approximately 1 h to perform. The method yielded 8-32 microg of high molecular weight DNA per 30 mg of freeze-dried mycelium when tested on six fungal species.

Pro TechEx- Genomic DNA Extraction From Fungi Teaching Kit

First lysing the cell and solubilizing the DNA, which is followed by enzymatic or chemical method to remove contaminating proteins, RNA and other macromolecules. The rigid cell wall of the plant cell is weakened before prceeding with further steps.

Pro TechEx-Genomic DNA Extraction From Plant Teaching Kit

DNA isolation techniques widely employed by plant molecular biologists use a CTAB (hexadecyltrimethyl ammonium bromide) extraction buffer coupled with reusable tissue homogenization systems such as a mortar and pestle followed by phenol chloroform treatment.

Pro TechEx-Genomic DNA Extraction From Plant Using CTAB Method Teaching Kit


Pro TechEx- Genomic DNA Extraction From Fungi Teaching Kit 10 Exps

20 Exps

2600.00

7500.00

OP-3122-10xp

OP-3122-20xp Pro TechEx- Genomic DNA Extraction From Fungi Teaching Kit


Pro TechEx-Genomic DNA Extraction From Plant Teaching Kit 5 Exps

10 Exps

2100.00

3150.00

OP-3123-5xp

OP-3123-10xp Pro TechEx-Genomic DNA Extraction From Plant Teaching Kit


Pro TechEx-Genomic DNA Extraction From Plant Using CTAB Method Teaching Kit 5 Exps

10 Exps

2700.00

3700.00

OP-3124-5xp

OP-3124-10xp Pro TechEx-Genomic DNA Extraction From Plant Using CTAB Method Teaching Kit

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COMPONENTS

RCLB (RT)100% Ethanol (RT)50X TAE (RT)70% Ethanol (RT)Agarose (RT)Ethidium Bromide (RT)WCLB (RT)

Gel Loading Dye (RT)DNA Marker (-20°C)Microfuge Tubes (RT)Saturation solution (RT)TE Buffer (RT)Protocol

COMPONENTS

Harju-Buffer(4°C)Chloroform(4°C)Precipitation solution(4°C)Wash solution (RT)RNase A (-20°C)Yeast culture (4°C)50X TAE (RT)

Agarose (RT)Ethidium bromide (RT)Gel loading dye (RT)TE buffer (RT)YPD (RT)Protocol

COMPONENTS

50X TAE (RT)Agarose (RT)column with 2ml collectiontube (RT)Elution buffer (4°C)Ethidium bromide (RT)

Gel loading dye (RT)Lysis buffer (4°C)TE Buffer (RT)Wash buffer (RT)Protocol

The Genomic DNA is Isolated from Whole Blood by using RBCL solution which lysis the RBC and WBC left intact. The white blood cells and their nuclear membrane are lysis by WBCL solution then the proteins and other cellular components are removed by saturation solution. Finally the high molecular weight Genomic DNA is precipitated by using absolute alcohol. The obtained Genomic DNA is resolved by using Agarose gel electrophoresis.

Pro TechEx-Genomic DNA Extraction From Whole Blood Teaching Kit

The Yeast Genomic DNA is isolated by Chloroform method. The cell wall is lysed by using lysis solution together with glass beads. It is followed by treatment with Phenol-Chloroform. Finally the DNA is precipitated by using ethanol. The obtained genomic DNA is resolved by using horizontal agarose gel electrophoresis.

Pro TechEx-Genomic DNA Extraction From Yeast Teaching Kit

The kit contains a membrane embedded in a spin column for binding up to 1-5ug of genomic DNA. Nucleotides, proteins, salts, and other impurities do not bind to the Column. Puri�ed genomic DNA can be applied in most molecular biology experiments including restriction digestion, PCR and other molecular technique.

Por TechEx-Genomic DNA Puri�cation By Using DNA Binding Membrane Teaching Kit

The green �uorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green �uorescence when exposed to light in the blue to ultraviolet range. The GFP from A.victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. The GFP gene can be introduced into organisms and maintained in their genome through breeding, injection with a viral vector, or cell transformation.

Por TechEx-GFP Cloning Teahing Kit


Pro TechEx-Genomic DNA Extraction From Whole Blood Teaching Kit 10 Exps

20 Exps

3250.00

4400.00

OP-3125-10xp

OP-3125-20xp Pro TechEx-Genomic DNA Extraction From Whole Blood Teaching Kit


Pro TechEx-Genomic DNA Extraction From Yeast Teaching Kit 10 Exps

20 Exps

3560.00

5200.00

OP-3126-10xp

OP-3126-20xp Pro TechEx-Genomic DNA Extraction From Yeast Teaching Kit




10 Exps

20 Exps

3900.00

7300.00

OP-3127-10xp

OP-3127-20xp

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COMPONENTS

Transformed culture (Control) (-20°C)T4 DNA Ligase (-20°C)10X Ligase buffer (-20°C)Insert DNA (-20°C)Vector DNA (-20°C)ATP (-20°C)Sterile water (-20°C)

Solution-I (4°C)Ampicillin (4°C)E.coli Host (4°C)LB broth (RT)Agar (RT)1.5 ml Microfuge tubes (RT)Protocol (RT)

COMPONENTS

K2HPO

4 (RT)

KH2PO

4 (RT)

Ammonium sulphate (RT)Tri-sodium citrate (RT)Agar (RT)

Glucose (RT)MgSO

4 (RT)

LB Broth (RT)E.coli host (4°C)Streptomycin (4°C)




5 Exps

10 Exps

5550.00

10200.00

OP-3128-5xp

OP-3128-10xp

In this method the colonies grown on glucose medium are overlay with 2, 3, 5 Triphenyl Tetrazolium chloride (TTC). After incubation the petite colonies remains white whereas the wild type colonies turned to red because of the reduction of tetrazolium chloride resulting formazan compound.

Pro TechEx-Isolation and characterization of petite mutant of Yeast Teaching Kit

Mutations often inactivate a biosynthetic pathway of the microorganism, and frequently make a microorganism unable to grow on a medium lacking an adequate supply of the pathway’s end product. Based on this principle microorganism are classi�ed as Prototrophic and Auxotrophic. Prototrophic organisms (wild type) have the same nutritional requirements as that of their ancestors. They need only inorganic salts, an organic energy source such as sugar, fat, protein and water to survive and grow. That is, the Prototroph’s need only "Minimal medium" for their growth and survival. Auxotrophic mutants are unable to grow without one or more essential nutrients. Auxotrophs are mutant for particular nutrient synthesis pathway enzymes. Such an error is known as an inborn error of metabolism, whether it occurs in a bacterium or a eukaryote. An auxotroph can be grown only on an enriched medium that provides the particular nutrient that the mutant cannot metabolize on its own.

Pro TechEx-Isolation of Antibiotic Resistant and Auxotrophic Mutant of Yeast Teaching Kit

Every gene manipulation procedure requires genetic material like DNA and RNA. Nucleic acids occur naturally in association with proteins and lipoprotein organelles. The dissociation of a nucleoprotein into nucleic acid and protein moieties and their subsequent separation, are the essential steps in the isolation of all species of nucleic acids. Isolation of nucleic acids is followed by quantitation of nucleic acids generally done by either spectrophotometric or by using �uorescent dyes (Ethidium Bromide)to determine the average concentrations and purity of DNA or RNA present in a mixture. Isolating the genetic material (DNA) from cells (bacterial, viral, plant or animal) involves three basic steps-

• Rupturing of cell membrane to release the cellular components and DNA• Separation of the nucleic acids from other cellular components• Puri�cation of nucleic acid

Pro Tech Ex- Isolation and Identi�cation of DNA and RNA Teaching Kit


Pro TechEx-Isolation and characterization of petite mutant of Yeast Teaching Kit 10 Exps 4200.00OP-3129-10xp

COMPONENTS

YPD agar (RT)Saccharomyces cerevisiae (4°C)Tetrazoilum Chloride (4°C)Protocol


Pro TechEx-Isolation of Antibiotic Resistant and Auxotrophic Mutant of Yeast Teaching Kit 5 Exps 4000.00OP-3130-5xp

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COMPONENTS

E.coli Strain (4°C)Standard DNA (4°C)Standard RNA (4°C)Trizol (4°C)Agarose (RT)Isopropanol(RT)Chloroform (RT)70%Ethanol (RT)

TE Buffer (RT)Gel Loading Dye (RT)Ethidium Bromide (RT)LB Broth (RT)50X TAE (RT)1.5ml Microfuge tubes (RT)Protocol

COMPONENTS

Agarose (RT)50X TAE (RT)Ethidium bromide (RT)Gel loading dye (RT)Lambda EcoR I digest (-20°C)

Ligase Buffer (-20°C)Ligase Enzyme (-20°C)Sterile water (RT)0.5ml tubes (RT)Protocol

COMPONENTS

50X TAE (RT)Agarose (RT)Ethidium Bromide (RT)Gel Loading Dye (RT)

Primer(F+R) (-20°C)Taq Polymerase (-20°C)Template DNA (-20°C)10 mM dNTP mix (-20°C)

10X Taq polymerase Buffer (-20°C)Distilled water (RT)Marker DNA (-20°C)PCR Tubes (RT)Protocol

COMPONENTS

0.5M EDTA (RT)10% SDS (RT)Ethyl alcohol (RT)Phenol (RT)TE Buffer (RT)

0.01M MgSO4 (RT)

Chloroform (RT)DNase I (-20°C)Phenol: Chloroform (4°C)RNAse (-20°C)

Maltose (20%) (4°C)SM buffer (4°C)NZY-amine medium (4°C)Protocol


Pro Tech Ex- Isolation and Identi�cation of DNA and RNA Teaching Kit 5 Exps

10 Exps

2950.00

4500.00

OP-3131-5xp

OP-3131-10xp Pro Tech Ex- Isolation and Identi�cation of DNA and RNA Teaching Kit


Pro TechEx-Ligation Teaching Kit 5 Exps

20 Exps

2300.00

6000.00

OP-3133-5xp

OP-3133-20xp Pro TechEx-Ligation Teaching Kit


Pro TechEx-Long PCR Teaching Kit 5 Exps

20 Exps

5350.00

17000.00

OP-3134-5xp

OP-3134-20xp Pro TechEx-Long PCR Teaching Kit

Viruses that infect prokaryotes are known as bacteriophages,or phages, because when they were �rst discovered,they appeared to eat bacterial cells, generating a clearing, or plaque, on a lawn of susceptible bacteria.In reality, the bacteria are killed by lysis as newly produced phages are released from the damaged cells

Pro TechEx-Lambda Phage DNA Isolation Teaching Kit

Joining linear DNA fragments together with covalent bonds is called ligation. More speci�cally, DNA ligation involves creating a phosphodi-ester bond between the 3' hydroxyl of one nucleotide and the 5' phosphate of another.The enzyme used to ligate DNA fragments is T4 DNA ligase, which originates from the T4 bacteriophage. This enzyme will ligate DNA fragments having overhanging,cohesive ends that are annealed together.

Pro TechEx-Ligation Teaching Kit

Long PCR Ampli�cation of extremely long PCR products (up to 40 kb DNA)Long Range PCR refers to the ampli�cation of DNA lengths that cannot typically be ampli�ed using routine PCR methods or reagents. For simple DNA templates, polymerases optimized for Long Range PCR can amplify up to 30 kb and beyond.

Pro TechEx-Long PCR Teaching Kit


Pro TechEx-Lambda Phage DNA Isolation Teaching Kit 10 Exps 4900.00OP-3132-10xp

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Mitochondrial DNA is only a small portion of the DNA in a eukaryotic cell; most of the DNA can be found in the cell nucleus, and in plants, the chloroplast as well. In humans, mitochondrial DNA can be assessed as the smallest chromosome coding for 37 genes and containing approximately 16,600 base pairs.

Mitochondrial DNA (mtDNA or mDNA) is the DNA located in mitochondria; cellular organelles within eukaryotic cells that convert chemical energy from food into a form that cells can use, adenosine triphosphate (ATP). Mitochondrial DNA is only a small portion of the DNA in a eukary-otic cell; most of the DNA can be found in the cell nucleus, and in plants, the chloroplast as well.

Pro TechEx-Mitochondrial DNA Isolation Teaching Kit

Multiplex PCR uses several pairs of primers annealing to different target sequences. This permits the simultaneous analysis of multiple targets in a single sample. For example, in testing for genetic mutations, six or more ampli�cations might be combined. In the standard protocol for DNA Fingerprinting, the targets assayed are often ampli�ed in groups of 3 or 4. Multiplex Ligation-dependent Probe Ampli�cation (or MLPA) permits multiple targets to be ampli�ed using only a single pair of primers, avoiding the resolution limitations of multiplex PCR. Multiplex PCR has also been used for analysis of microsatellites and SNPs.

Pro TechEx-Multiplex PCR Teaching Kit

Mutation occur due to deletion, insertion or duplication of bases. Mutation Can be detected by restiction enzyme mapping. Insertion, deletion or duplication of a base may create a new restiction enzyme site.

Pro TechEx-Mutation Detection and Analysis (Mutagenesis) Teaching Kit


Pro TechEx-Mitochondrial DNA Isolation Teaching Kit 10 Exps 9500.00OP-3135-10xp

COMPONENTS

BME(4°C)BSA(4°C)Isopropanol (RT)ST Buffer (4°C)STE Buffer (4°C)TE Saline Buffer (4°C)

Ammonium acetate (RT)Chloroform /Isoamyl alcohol (4°C)DNase I (-20°C)Magnesium acetate (RT)Phenol/Chloroform (4°C)Tris saturated phenol (4°C)

Ethanol II (RT)EDTA (RT)Ethanol I (RT)SDS (RT)Potassium acetate (RT)Protocol


Pro TechEx-Multiplex PCR Teaching Kit 5 Exps

25 Exps

4500.00

18100.00

OP-3136-5xp

OP-3136-25xp Pro TechEx-Multiplex PCR Teaching Kit


Pro TechEx-Mutation Detection and Analysis (Mutagenesis) Teaching Kit 5 Exps

10 Exps

3500.00

6000.00

OP-3137-5xp

OP-3137-10xp Pro TechEx-Mutation Detection and Analysis (Mutagenesis) Teaching Kit

COMPONENTS

Agarose (RT)50X TAE (RT)Ethidium Bromide (RT)Gel Loading Dye (RT)Template DNA (-20°C)Multiplex Primer(F+R) (-20°C)Taq Polymerase (-20°C)

10 mM dNTP mix (-20°C)10X Taq polymerase Buffer (-20°C)Distilled water (RT)Marker DNA (-20°C)PCR Tubes (RT)Protocol

COMPONENTS

Agarose (RT)Control DNA (-20°C)DNA (Mutated) (-20°C)Ethidium Bromide (RT)Restriction Enzyme (-20°C)

50X TAE (RT)Gel loading Dye (RT)Restriction Buffer (-20°C)Protocol

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Nested PCR used to increase the speci�city of DNA ampli�cation. Two sets of primers are used in two successive reactions. In the �rst PCR, one pair of primers is used to generate DNA products, which may contain products ampli�ed from non-target areas. The products from the �rst PCR are then used as template in a second PCR, using one ('hemi-nesting') or two different primers whose binding sites are located (nested) within the �rst set, thus increasing speci�city. Nested PCR is often more successful in speci�cally amplifying long DNA products than conven-tional PCR, but it requires more detailed knowledge of the Sequence of the target.

Pro TechEx-Nested PCR Teaching Kit

The basic repeating unit of chromatin, the nucleosome, is known to play a critical role in regulating the process of gene transcription. The positioning of nucleosomes on a promoter is a signi�cant determinant in its responsiveness to gene-inducing signals. For example, positioning and subsequent mobilization of nucleosomes can regulate the access of various DNA factors to underlying DNA templates. Several mecha-nisms have been proposed to direct the process of nucleosome displacement such as chemical histone modi�cations, ATP-dependent remod-elling, and the incorporation of histone variants. Thus, rather than being an inert molecular structure, chromatin is highly dynamic in response to the transcription process. In this section, we describe two methodologies that allow the determination of exact nucleosome positioning within speci�c gene regions.

Pro TechEx-Nucleosome Mapping by Micrococcal Nulease (MNase) Digestion Teaching Kit

Oligonucleotide synthesis involves a large number of individual reactions, which leads inevitably to the accumulation of impurities such as truncated oligonucleotides and sequences containing chemically-modi�ed bases. These impurities must be removed after synthesis

Pro TechEx-Oligonucleotide Puri�cation Teaching Kit


Pro TechEx-Nested PCR Teaching Kit 5 Exps

25 Exps

5200.00

19000.00

OP-3138-5xp

OP-3138-25xp Pro TechEx-Nested PCR Teaching Kit

COMPONENTS

Agarose (RT)50X TAE (RT)Ethidium Bromide (RT)Gel Loading Dye (RT)Template DNA (-20°C)Primer(F+R) (-20°C)Taq Polymerase (-20°C)

10mM dNTP mix (-20°C)10X Taq polymerase Buffer (-20°C)Distilled water (RT)Marker DNA (-20°C)PCR Tubes (RT)Protocol




5 Exps

20 Exps

5500.00

15500.00

OP-3139-5xp

OP-3139-20xp




5 Exps

10 Exps

6700.00

11000.00

OP-3140-5xp

OP-3140-10xp

COMPONENTS

2X TNSEK solution (RT)Nuclear Buffer I (-20°C)Nuclear Buffer II (-20°C)Nuclear Buffer III (-20°C)PBS (RT)

0.1M CaCl2 (4°C)

MNase (-20°C)NaOH 1M (RT)Protocol

COMPONENTS40% polyacrylamide (4°C)TEMED (4°C)APS (4°C)Oligonucleotide I& II (-20°C)Urea (RT)Butanol (RT)Phenol :chloroform (4°C)Loading buffer (4°C)

TE buffer (4°C)Ethanol I (RT)Ethanol II (RT)TLC plate (RT)10X TEB (RT)UV wrap (RT)Protocol

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The factors (inducer) which in�uence the expression of foreign proteins in Escherichia coli under the transcriptional control of the lac and tac promoters, and discusses conditions for maximizing the production of a foreign protein using this system. Speci�cally, the in�uence of an inducer IPTG (isopropyl-β-D-thiogalactoside) concentration, temperature, composition of the growth medium, the point in the growth curve at which cells are induced with either IPTG or lactose, and the duration of the induction phase are discussed.

Pro TechEx-Optimization of time of Inducer Concentration on E.coli Recombinant Protein Expression Teaching Kit

The size of pUC19 will be increased to 5755bp with the addition of lacZ gene, which encodes β-galactosidase. The lacZ gene was ampli�ed by PCR method. The ampli�ed sequence was transformed to E.coli host .The production of β-galactosidase enzyme was analysed by SDS - PAGE method.

Pro TechEx-PCR Ampli�cation of β-Galactosidase Gene Teaching Kit

The polymerase chain reaction (PCR) has found widespread application in many areas of genetic analysis. For most, if not all of these applications, alternative methods of DNA (or RNA) ampli�cation, can be substituted.The �rst application of PCR was for genetic testing, where a sample of DNA is analyzed for the presence of genetic disease mutations. Prospective parents can be tested for being genetic carriers, or their children might be tested for actually being affected by a disease. DNA samples for prenatal testing can be obtained by amniocentesis, chorionic villus sampling, or even by the analysis of rare fetal cells circulating in the mother's bloodstream. PCR analysis is also essential to preimplantation genetic diagnosis, where individual cells of a developing embryo are tested for mutations.

Pro TechEx-PCR Application Teaching Kit

PCR is a revolutionary method developed by Kary Mullis in the 1980s. PCR is based on using the ability of DNA polymerase to synthesize new strand of DNA complementary to the offered template strand. Because DNA polymerase can add a nucleotide only onto a preexisting 3'-OH group, it needs a primer to which it can add the �rst nucleotide. This requirement makes it possible to delineate a speci�c region of template sequence that the researcher wants to amplify. At the end of the PCR reaction, the speci�c sequence will be accumulated in billions of copies (amplicons).

Pro TechEx-PCR Teaching Kit


Pro TechEx-Optimization of time of Inducer Concentration on E.coli Recombinant Protein Expression Teaching Kit 5 Exps 6000.00OP-3141-5xp


Pro TechEx-PCR Ampli�cation of β-Galactosidase Gene Teaching Kit 5 Exps 4350.00OP-3142-5xp

COMPONENTS

ONPG (4°C)Buffer A (RT)E.coli (4°C)Inducer (20mg/ml) (-20°C)Lysis Buffer (RT)

1.5ml Microfuge tube (RT)LB broth (RT)Stop Solution (RT)β-Mercaptoethanol (RT)Protocol

COMPONENTS

Agarose (RT)50X TAE (RT)Ethidium Bromide (RT)Gel Loading Dye (RT)Template DNA (-20°C)Primer (-20°C)Taq Polymerase (-20°C)


COMPONENTS

Control DNA I (-20°C)Control DNA II (-20°C)Master mix vials (-20°C)Random primer (-20°C)Taq polymerase (-20°C)Test DNA (-20°C)

1Kb DNA ladder (-20°C)50X TAE (RT)Agarose (RT)Ethidium bromide (RT)Gel loading Dye (RT)Protocol


Pro TechEx-PCR Application Teaching Kit 5 Exps

20 Exps

4800.00

17800.00

OP-3143-5xp

OP-3143-20xp Pro TechEx-PCR Application Teaching Kit

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Pro TechEx-PCR Teaching Kit 10 Exps

20 Exps

4300.00

6500.00

OP-3144-10xp

OP-3144-20xp Pro TechEx-PCR Teaching Kit

COMPONENTS

Agarose (RT)50X TAE (RT)Ethidium Bromide (RT)Gel Loading Dye (RT)Template DNA (-20°C)Primer(F+R) (-20°C)Taq Polymerase (-20°C)


The number of phage particles contained in the original stock phage culture is determined by counting the number of plaques formed on the seeded agar plate and multiplying this by the dilution factor. For a valid phage count, the number of plaques per plate should not exceed 300 nor be less than 30. Plates showing greater than 300 PFUs are too numerous to count (TNTC); plates showing fewer than 30 PFUs are too few to count (TFTC).

Pro TechEx-Phage Titration Teaching Kit

Bacterial plasmids are known to harbor genes for: (i) resistances to antibiotics and metals, (ii) catabolic pathways such as lactose utilization and degradation of hydrocarbons (iii) biosynthesis of certain antibiotics, etc. In many cases the characteristics of the host organism conferred by the plasmids remain elusive, and such cryptic plasmids are abundant in nature. Curing of this cryptic plasmid from a bacterial strain is a method to substantiate the relationship between a genetic trait and carriage of that speci�c trait in the plasmid. Various methods involving chemical and physical agents have been developed to eliminate plasmids. Protocols for curing plasmids consist frequently of exposure of a culture to sub-inhibitory concentrations of some chemical agents, e.g. acridine orange, acri�avine, and sodium dodecyl sulfate or to a super-op-timal temperature followed by selection of cured derivatives.

Pro TechEx-Plasmid Curing Teaching Kit

Plasmid is a double stranded, circular extra chromosomal DNA of bacterium. It is used in recombinant DNA experiments to clone genes from other organisms and make large quantities of their DNA. Plasmid can be transferred between same species or between different species. Size of plasmids range from 1-1000 kilo base pairs. Plasmids are part of mobilomes (total of all mobile genetic elements in a genome) like transpos-ons or prophages and are associated with conjugation. Even the largest plasmids are considerably smaller than the chromosomal DNA of the bacterium, which can contain several million base pairs.

Pro TechEx- Plasmid DNA Isolation Teaching Kit

COMPONENTS

10X SM Buffer (RT)20% Maltose solution (RT)Agar (1.5 g /100 ml) (RT)Sodium Chloride (RT)Tryptone (RT)

20% Magnesium Chloride (RT)E.coli Host (4°C)LB broth (RT)Phage lysate (4°C)Protocol


Pro TechEx-Phage Titration Teaching Kit 5 Exps

20 Exps

3600.00

9500.00

OP-3145-5xp

OP-3145-20xp Pro TechEx-Phage Titration Teaching Kit

COMPONENTS

E.coli host (4°C)50X TAE (RT)6X Gel loading dye (RT)Agar (RT)Agarose (RT)LB broth (RT)

Ampicillin (4°C)Chloroform (4°C)Ethidium Bromide (RT)Microfuge Tubes (RT)Phenol (4°C)Protocol


Pro TechEx-Plasmid Curing Teaching Kit 10 Exps

20 Exps

2800.00

4100.00

OP-3146-10xp

OP-3146-20xp Pro TechEx-Plasmid Curing Teaching Kit

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COMPONENTS

Alkaline lysis solution I (RT)Alkaline lysis solution II (RT)Alkaline lysis solution III (RT)Control Plasmid DNA (-20°C)Isopropanol (RT)E.coli Culture (-20°C)TE (pH 8.0)

RNase A (-20°C)50X TAE (RT)70% Ethanol (RT)Agarose (RT)Ethidium Bromide (RT)Gel loading buffer (RT)Protocol

COMPONENTS

2ml Collection Vial (RT)Column (RT)Elution Buffer (RT)Gel loading dye (RT)Solution I (RT)Solution II (RT)Solution III (RT)Wash Solution (RT)

50X TAE (RT)Agarose (RT)Ampicillin (4°C)DNA Marker (-20°C)E.coli Host (4°C)Ethidium Bromide (RT)RNase A (-20°C)Protocol

COMPONENTS

70% ethanol (RT)Holding solution (RT)Enzyme digest solution (4°C)Dilution solution I (RT)Dilution solution II (RT)

Agglutination solution (RT)Glass slide (RT)Cover slips (RT)1.5ml centrifuge tubes (RT)Protocol


Pro TechEx- Plasmid DNA Isolation Teaching Kit 5 Exps

20 Exps

3200.00

4500.00

OP-3147-5xp

OP-3147-20xp Pro TechEx- Plasmid DNA Isolation Teaching Kit


Pro TechEx- Plasmid Puri�cation By DNA Binding Membrane Teaching Kit 10 Exps 2400.00OP-3148-10xp


Protech Ex - Protoplast fusion Teaching kit 5 Exps 1500.00OP-3149-5xp

After harvesting and resuspension, the bacterial cells are lysed in NaOH-SDS in the presence of RNase A. SDS solubilizes the phospholipid and protein components of the cell membrane, leading to lysis and release of the cell contents. NaOH denatures the chromosomal and plasmid DNAs, as well as proteins. The optimized lysis time allows maximum release of plasmid DNA from the cell without release of cell wall-bound chromosomal DNA, while minimizing the exposure of the plasmid to denaturing conditions. Long exposure to alkaline conditions may cause the plasmid to become irreversibly denatured. This denatured form of the plasmid runs faster on agarose gels and is resistant to restriction enzyme digestion. The lysate is neutralized by the addition of acidic potassium acetate. The high salt concentration causes KDS* to precipitate, and the denatured proteins, chromosomal DNA, and cellular debris become trapped in salt–detergent complexes.

Pro TechEx- Plasmid Puri�cation By DNA Binding Membrane Teaching Kit

An improved procedure has been developed for the fusion of plant protoplasts based on the use of polyethylene glycol possessing a low carbonyl content. Different commercial preparations of PEG were analysed using a sensitive carbonyl assay procedure and only those with a very low carbonyl content (< 5 × 10−4M) were used. Sterilisation by membrane �ltration was employed, since autoclaving markedly increased the carbonyl content with associated deleterious effects on fusion frequency and heterokaryon viability. Elution of PEG was carried out using a hypotonic mannitol solution at pH 5.8, without added Ca2+, immediately followed by 9 % w/v mannitol (pH 5.8), again without added Ca2+. This improved PEG fusion procedure has been found to be applicable to a range of protoplast combinations. Its use results in a high fusion frequency with good resultant heterokaryon viability and a low level of clumping.

Protech Ex - Protoplast fusion Teaching kit

A procedure for extracting plasmid DNA from bacterial cells is described. The method is simple enough to permit the analysis by gel electro-phoresis.

Pro TechEx-Rapid Plasmid Screening Teaching Kit

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COMPONENTS

E.coli host (4°C)50X TAE (RT)6X gel loading dye (RT)Agar (RT)Agarose (RT)LB-Broth (RT)

Ampicillin (4°C)Ethidium Bromide (RT)Microfuge tube (RT)Solution I (4°C)Solution II (4°C)Protocol

COMPONENTS

ONPG (4°C)Buffer A (RT)E.coli (4°C)Lysis Buffer (RT)Mercaptoethanol (RT)

1.5ml Microfuge tube (RT)LB broth (RT)Stop Solution (RT)Protocol




5 Exps 1800.00

20 Exps 4400.00

OP-3150-5xp

OP-3150-20xp


Pro TechEx-Reporter Gene Assay Teaching Kit 5 Exps 8000.00OP-3151-5xp

COMPONENTS

Agarose (RT)50X TAE (RT)Gel loading Dye (RT)Ethidium Bromide (RT)0.5ml Microfuge tubes (RT)pBR322 (-20°C)

pUC18 (-20°C)HindIII (-20°C)Restriction Buffer (-20°C)Distilled Water (-20°C)Protocol

COMPONENTS

50X TAE (RT)Agarose (RT)Ethidium bromide (RT)Gel loading dye (RT)Template DNA (-20°C)

0.5ml tubes (RT)Enzyme Buffer (-20°C)Hind III (-20°C)Sterile water (RT)Protocol


Pro TechEx-Restriction Analysis of Plasmid (pBR322 & pUC18) Teaching Kit 5 Exps 6700.00OP-3152-5xp

Genetic reporters are used as indicators to study gene expression and cellular events coupled to gene expression. Following transfer, the cells are assayed for the presence of the reporter by directly measuring the reporter protein itself or the enzymatic activity of the reporter protein.

Pro TechEx-Reporter Gene Assay Teaching Kit

Restriction enzymes (Hind III) are Nucleases which can cleave the sugar-phosphate backbone of pBR322 & pUC18, found in bacteria. As they cut within the molecule, they are commonly called restriction endonucleases. They speci�cally cleave the nucleic acids at speci�c nucleotide sequence called Restriction sites to generate a set of smaller fragments.

Pro TechEx-Restriction Analysis of Plasmid (pBR322 & pUC18) Teaching Kit

Restriction enzymes are Nucleases which can cleave the sugar-phosphate backbone of DNA, found in bacteria. As they cut within the molecule, they are commonly called restriction endonucleases. They speci�cally cleave the nucleic acids at speci�c nucleotide sequence called Restriction sites to generate a set of smaller fragments.

Pro TechEx-Restriction Digestion Teaching Kit


Pro TechEx-Restriction Digestion Teaching Kit 5 Exps

20 Exps

2600.00

5600.00

OP-3153-5xp

OP-3153-20xp Pro TechEx-Restriction Digestion Teaching Kit

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Pro TechEx-RT-PCR Teaching Kit 5 Exps 7100.00OP-3155-5xp

Restriction mapping is the process of obtaining structural information on a piece of DNA by the use of restriction enzymes. Generating such a map is usually the �rst step in characterizing an unknown DNA, and a prerequisite to manipulating it for other purposes. Typically, restriction enzymes that cleave DNA infrequently (e.g. those with 6bp recognition sites) and are relatively inexpensive are used to produce at a map.

Pro TechEx-Restriction Mapping Teaching Kit

Reverse transcription polymerase chain reaction (RT-PCR) is one of many variants of polymerase chain reaction (PCR). This technique is commonly used in molecular biology to detect RNA expression. RT-PCR is often confused with real-time polymerase chain reaction (qPCR) by students and scientists alike. However, they are separate and distinct techniques. While RT-PCR is used to qualitatively detect gene expression through creation of complementary DNA (cDNA) transcripts from RNA, qPCR is used to quantitatively measure the ampli�cation of DNA .

Pro TechEx-RT-PCR Teaching Kit

Genetic disorders and other related diseases are often associated with Single Nucleotide Polymorphism (SNP) occurring at precise positions on genes/ genomes. Development of SNP-speci�c primers that amplify a speci�c fragment in PCR when a speci�c SNP is present helps in disease detection.SNP detection where two DNA templates, one wild-type and the other template with SNP are used for PCR ampli�cation individually along with 2 pairs (4 numbers) of primers, one pair speci�c to wild-type and the other, speci�c to SNP-type primer.

Pro TechEx-Single Nucleotide Polymorphisms (SNP) Teaching Kit

Site-directed mutagenesis is a molecular biology method that is used to make speci�c and intentional changes to the DNA sequence of a gene and any gene products. Also called site-speci�c mutagenesis or oligonucleotide-directed mutagenesis, it is used for investigating the structure and biological activity of DNA, RNA, and protein molecules, and for protein engineering. Site-directed mutagenesis is one of the most important techniques in laboratory for introducing mutation into a DNA sequence. However, with decreasing costs of oligonucleotide synthesis, arti�cial gene synthesis is now occasionally used as an alternative to site-directed mutagenesis.

Pro TechEx-Site Directed Mutagenesis by PCR Teaching Kit


Pro TechEx-Restriction Mapping Teaching Kit 5 Exps

20 Exps

3250.00

6400.00

OP-3154-5xp

OP-3154-20xp Pro TechEx-Restriction Mapping Teaching Kit

COMPONENTS

50X TAE Buffer (RT)Agarose (RT)Distilled Water (RT)Ethidium Bromide (RT)Gel loading dye (RT)

DNA (-20°C)ECoR I (-20°C)Hind III (-20°C)Restriction Buffer (-20°C)Protocol


Pro TechEx-Single Nucleotide Polymorphisms (SNP) Teaching Kit 5 Exps

20 Exps

5400.00

17800.00

OP-3156-5xp

OP-3156-20xp Pro TechEx-Single Nucleotide Polymorphisms (SNP) Teaching Kit

COMPONENTS

Control DNA (-20°C)DNA Marker (-20°C)dNTP Mix (-20°C)Primer Mix* (-20°C)SNP DNA (-20°C)Taq DNA Polymerase(-20°C)Taq DNA PolymeraseBuffer 10X (-20°C)

0.2ml PCR Tube (RT)50X TAE (RT)Agarose (RT)Distilled water (RT)Ethidium Bromide (RT)Gel loading Dye (RT)Protocol

COMPONENTS

RNA Template (-20°C)0.2ml PCR tubes (RT)10mM DTT (-20°C)50X TAE (RT)Agarose (RT)Ethidium Bromide (RT)Gel Loading dye (RT)

DNA Marker (-20°C) Primer F (-20°C) Primer R (-20°C)Random Primer (-20°C)Rnase Free Water (-20°C)Taq Polymerase (-20°C)10mM dNTP (-20°C)

5x Reverse Transcriptase Buffer (-20°C)M-MuLV Reverse transcriptase (-20°C)Ribonuclease Inhibitor (-20°C)Taq Polymerase Buffer (-20°C)Protocol

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COMPONENTS

dNTP mix (-20°C)Primer (MF+R) (-20°C)Restriction enzyme (-20°C)Strile water (-20°C)Taq polymerase (-20°C)Taq polymerase buffer (-20°C)Template DNA (-20°C)

Ampicillin (4°C)CaCl

2 (4°C)

E.coli Host (4°C)IPTG (-20°C)Restriction buffer (-20°C)X-gal (-20°C)LB broth (RT)

0.2ml PCR Tubes (RT)Agar (RT)Protocol


Pro TechEx-Site Directed Mutagenesis by PCR Teaching Kit 5 Exps 4900.00OP-3157-5xp


Pro TechEx-Southern Hybridization Teaching Kit 5 Exps 7500.00OP-3159-5xp


Pro TechEx-Study of DNA Methylation Teaching Kit 5 Exps 4800.00OP-3160-5xp

COMPONENTS

EcoRI Methylase Enzyme (-20°C)10X Restriction Buffer (-20°C)Lambda DNA (-20°C)Methylase Buffer (10X) (-20°C)Restriction enzyme (EcoRI) (-20°C)S-Adenosyl methionine (SAM) (-20°C)50X TAE (RT)

Agarose (RT)Distilled Water(-20°C)Ethidium Bromide (RT)Gel loading Dye (RT)Microfuge Tubes (RT)Protocol

COMPONENTS

10X Safe Blue DNA Stain (-20°C)50X TAE (RT)Agarose (RT)Control DNA (RT)DNA Sample I (RT)DNA Sample II (RT)EthidiumBromide(RT)10X Substrate (RT)

5X Wash Buffer-A (RT)5X Wash Buffer-B (RT)Biotinylated Probe (RT)HRP-Conjugate (RT)Hybridization Buffer (RT)Prehybridization Buffer (RT)50X Electrotransfer Buffer (RT)5X Blocking Buffer (RT)

5X Wash Buffer-C (RT)Blocking Powder (RT)Casein (RT)Control Nylon Membrane (RT)Nylon Membrane (RT)Protocol

Southern blotting was named after Edward M. Southern who developed this procedure at Edinburgh University in the 1975. It allows investi-gators to determine the molecular weight of a restriction fragment, to measure relative amounts in different samples and to locate a particular sequence of DNA within a complex mixture. DNA (genomic or other source) is digested with a restriction enzyme and separated by gel electro-phoresis and transferred from an agarose gel onto a membrane.

Pro TechEx-Southern Blotting Teaching Kit

Hybridization involves several steps. First, the probe and sample DNA are allowed to hybridize under the appropriate conditions. The correct temperature, incubation times, and buffer conditions must be used. Next, the sample DNA is washed using conditions that will remove unhybridized probe but not the hybridized probe. Finally, the sample DNA is tested for the presence of the hybridized probe. The probe is labeled with a radioactive molecular tag (or some other tag) that allows it to be detected following hybridization.

Pro TechEx-Southern Hybridization Teaching Kit

DNA methylation is a biochemical process where a methyl group is added to the cytosine or adenine DNA nucleotides.

Pro TechEx-Study of DNA Methylation Teaching Kit


Pro TechEx-Southern Blotting Teaching Kit 5 Exps

20 Exps

5200.00

17300.00

OP-3158-5xp

OP-3158-20xp Pro TechEx-Southern Blotting Teaching Kit

COMPONENTS

50X TAE (RT)Agarose (RT)DNA sample (-20°C)Ethidium Bromide (RT)Gel loading dye (RT)

20X SSC (RT)Nitrocellulose Membrane (RT)Protocol

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DNA repair is a collection of processes by which a cell identi�es and corrects damage to the DNA molecules that encode its genome.DNA in the living cell is subject to many chemical alterations. If the genetic information encoded in the DNA is to remain uncorrupted, any chemical changes must be corrected. A failure to repair DNA produces a mutation.

Pro TechEx-Study of DNA Repair Teaching Kit

UV light which has a lower energy content than ionizing radiations, are capable of producing lethal effects in cells exposed to low penetrat-ing wavelengths in range of 210 to 300nm. Cellular components capable of absorbiong UV light are nucleic acids with the DNA as the primary site of damage.this results in the inabilityy to form viable cells after exposure to UV radiation. Hydroxylamine is used to mutagenize DNA in Vitro when used in vitro , Hydroxylamine reacts with cytosine , converting it to a modi�ed base that pairs with adenine.This has two consequences that is Hydroxylamine only produces G:C to A:T transitions , and mutations induced by hydroxylamine cannot be reverte

Pro TechEx-Study of Physical and Chemical Mutagens on The Survival of E.coli Growth Teaching Kit

Pro TechEx-Transduction Teaching Kit

In molecular biology, transformation is the genetic alteration of a cell resulting from the direct uptake and incorporation of exogenous genetic material from its surroundings and taken up through the cell membrane. Transformation occurs naturally in some species of bacteria, but it can also be affected by arti�cial means in other cells. For transformation to happen, bacteria must be in a state of competence, which might occur as a time-limited response to environmental conditions such as starvation and cell density. "Transformation" may also be used to describe the insertion of new genetic material into nonbacterial cells, including animal and plant cells; however, because "transformation" has a special meaning in relation to animal cells, indicating progression to a cancerous state, the term should be avoided for animal cells when describing introduction of exogenous genetic material.

Pro TechEx-Transformation (Blue - White Screening) Teaching Kit

COMPONENTS

0.2ml PCR Tubes (RT)50X TAE (RT)Agarose (RT)EtBr (RT)Gel loading dye (RT)Taq Polymerase (-20°C)UV Damaged Lambda DNA (-20°C)dNTP (-20°C)

Double distilled water (-20°C)Lambda DNA (-20°C)NAD+ (-20°C)Primer(F+R) (-20°C)Repair Enzyme Mix (-20°C)Thermopolymerase buffer (-20°C)Protocol


Pro TechEx-Study of DNA Repair Teaching Kit 5 Exps 5800.00OP-3161-5xp


Pro TechEx-Study of Physical and Chemical Mutagens on The Survival of E.coli Growth Teaching Kit 5 Exps 4300.00OP-3162-5xp

COMPONENTS

Agar (RT)E.coli host (4°C)Hydroxylamine (RT)LB broth (RT)

Saline (RT)sodium hydroxide (RT)Protocol

COMPONENTS

Ampicillin (4°C)Donar strain (-20°C)Host strain (-20°C)Kanamycin (4°C)Phage lysate (-20°C)Recipient strain (-20°C)

10% Dextrose (4°C)1M Calcium chloride (RT)1M Magnesium chloride (RT)Agar (RT)LB Broth (RT)Protocol


Pro TechEx-Transduction Teaching Kit 5 Exps

20 Exps

4850.00

8500.00

OP-3163-5xp

OP-3163-20xp Pro TechEx-Transduction Teaching Kit

Transduction is the process by which DNA is transferred from one bacterium to another by a virus. It also refers to the process whereby foreign DNA is introduced into another cell via a viral vector. Transduction does not require physical contact between the cell donating the DNA and the cell receiving the DNA (which occurs in conjugation), and it is DNase resistant (transformation is susceptible to DNase). Transduction is a common tool used by molecular biologists to stably introduce a foreign gene into a host cell's genome.

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Pro TechEx-Transformation (Blue - White Screening) Teaching Kit 5 Exps 3600.00OP-3164-5xp


Pro TechEx-Transformation of Yeast Cell Using lithium acetate Teaching Kit 10 Exps

20 Exps

5100.00

7800.00

OP-3165-10xp

OP-3165-20xp Pro TechEx-Transformation of Yeast Cell Using lithium acetate Teaching Kit


Pro TechEx-Yeast Plasmid DNA Extraction Teaching Kit 5 Exps

20 Exps

2750.00

3800.00

OP-3166-5xp

OP-3166-20xp Pro TechEx-Yeast Plasmid DNA Extraction Teaching Kit

COMPONENTS

Control TransformedBacteria culture (-20°C)Host Bacteria (-20°C)LB Agar (RT)LB broth (RT)Plasmid DNA (-20°C)Solution- A (4°C)

1.5ml Microfuge tubes (RT)Ampicillin (4°C)Glycerol(Sterile) (4°C)IPTG (-20°C)X-gal (-20°C)Protocol

COMPONENTS

Ampicillin (4°C)Yeast culture (4°C)pUC19 (Plasmid) (-20°C)Transformation buffer (4°C)Glycerol+CaCl

2 (4°C)

YPD (RT)X-Gal (-20°C)IPTG (-20°C)Protocol

COMPONENTS

Ampicillin (4°C)E.coli Host (4°C)Cacl

2 (4°C)

Solution I (RT)Solution II (RT)Yeast Culture (4°C)

Agar (RT)Glass Beads (RT)LB Broth (RT)Microfuge tubes (RT)YPD Broth (RT)Protocol

The genetic transformation of target cells is a key tool in modern biological research, as well as in many gene therapy and biotechnology applications. Here we describe a new method for delivery of DNA into several industrially important species of yeast, including Saccharomyces cerevisiae.

Pro TechEx-Transformation of Yeast Cell Using lithium acetate Teaching Kit

A procedure for the rapid isolation of DNA from the yeast Saccharomyces cerevisiae is described. To release plasmid DNA for the transfor-mation of Escherichia coli , cells are subjected to vortex mixing in the presence of acid-washed glass beads, Triton X-100, sodium dodecyl sulfate, phenol and chloroform. Centrifugation of this mixture separates the DNA from cellular debris. E. coli can be ef�ciently transformed with plasmid present in the aqueous layer without further puri�cation of the plasmid DNA. This procedure also releases chromosome DNA. Following two ethanol precipitations, the chromosomal DNA can be digested by restriction endonucleases poly A adenylation to mRNA.

Pro TechEx-Yeast Plasmid DNA Extraction Teaching Kit

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This kit is designed to understand the structure of proteins by following technique listed below:

1. Study the role of disulphide bonds in maintaining protein structure.2. Role of heat denaturation on protein structure.3. Use of electrophoresis for characterization of proteins.4. Use of SDS-PAGE for analysis of Protein structure.

Labeling antibodies by covalent coupling of a biotinyl group is simple and normally does not have any adverse effect on the antibody. Most biotinylations are performed using ester of biotin. The reagent reacts with primary amines of the lysine residues or the amino terminus on the antibody to form amide bonds. A water soluble analog of N-hydroxylsuccinimide biotin also used in these procedures.

Pro TechEx-Analysis of Protein Structure Teaching Kit

Pro TechEx-Antibody Biotin Conjugation Teaching Kit

FITC is most commonly-used �uorescent dye for FACS analysis. FITC is a small organic molecule, and is typically conjugated to proteins via primary amines (i.e., lysines). Usually, between 3 and 6 FITC molecules are conjugated to each antibody; higher conjugations can result in solubility problems as well as internal quenching (and reduced brightness). Thus, an antibody will usually be conjugated in several parallel reactions to different amounts of FITC, and the resulting reagents will be compared for brightness (and background stickiness) to choose the optimal conjugation ratio. Fluorescein is typically excited by the 488 nm, and emission is collected at 530 nm

Pro TechEx-Antibody FITC Conjugation Teaching Kit

COMPONENTS

10% SDS (RT)Destainer (RT)Running buffer (RT)Separating Buffer(RT)Stacking buffer (RT)Stainer (RT)

Acrylamide (4°C)APS (4°C)Microfuge tubes(RT)Reducing agent (4°C)Sample solubilizing buffer (4°C)TEMED (4°C)

Protein I (-20°C)Protein II(-20°C)Protein marker I(-20°C)Protein marker II(-20°C)Protein marker III(-20°C)Protocol

COMPONENTS

Antibody (-20°C)Antigen Spotted Strip(4°C)Biotin (-20°C)Coupling buffer I (4°C)Coupling buffer II (4°C)Streptavidin ALP(-20°C)

10X PBS (RT)BCIP/NBT (4°C)Dialysis Bag (RT)Dialysis Clip (RT)Washing Buffer (RT)Protocol

COMPONENTS

Antibody (-20°C)Antigen Spotted Strip(4°C)FITC(-20°C)Mounting buffer (4°C)Glass Slides (RT)Substrate(4°C)

10X PBS (RT)BCIP/NBT (4°C)Dialysis Bag (RT)Dialysis Clip (RT)Washing Buffer (RT)Protocol


Pro TechEx-Analysis of Protein Structure Teaching Kit 5 Exps

15 Exps

3300.00

6200.00

OP-3201-5xp

OP-3201-15xp Pro TechEx-Analysis of Protein Structure Teaching Kit


Pro TechEx-Antibody Biotin Conjugation Teaching Kit 5 Exps 5600.00OP-3202-5xp


Pro TechEx-Antibody FITC Conjugation Teaching Kit 5 Exps 3400.00OP-3203-5xp

PROTEOMICS

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COMPONENTS

10X PBS (RT)Antibody(-20°C)Blocker(RT)Horse radishperoxidase(HRP)(-20°C)Hydrogen peroxide (H2O2)(RT)Pre-coated strips(4°C)Substrate(4°C)

96-Well Plate(RT)Dialysis bag(RT)Dialysis clip(RT)Glutaraldehyde(RT)Stopping solution(RT)Washing buffer(RT)Protocol

COMPONENTS

1.5ml Microfuge Tubes (RT)LB Broth (RT)Lysis Buffer (RT)Reaction Buffer (RT)Stopping Solution (RT)

E.coli host (4°C)Inducer (-20°C)Substrate (-20°C)Protocol

COMPONENTS

Stainer(RT)Destainer(RT)Protein sample(RT)Running buffer(RT)Cellulose acetate strip(RT)Protocol

Antibody HRP Conjugate Kit contains a horseradish peroxidase-conjugated Monoclonal Antibody that is useful as a single detection reagent, thus eliminating cross-reactivity associated with secondary antibody reagents. It recognizes histidine residues regardless of the surrounding amino acid content. The puri�ed antibody conjugate produces a strong signal at a 1:1000-1:2000 dilution on Western blots and dot blots. Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make them useful for detection, puri�cation or sorting applications.

Pro TechEx-Antibody HRP Conjugation Teaching Kit

Alkaline phosphatase (ALP) catalyzes the hydrolysis of phosphate esters in alkaline buffer and produces an organic radical and inorganic phosphate. Changes in alkaline phosphatase level and activity are associated with various disease states in the liver and bone. Kit uses p-ni-trophenyl phosphate (pNPP) as a phosphatase substrate which turns yellow (λmax= 405 nm) when dephosphorylated by ALP. The Kit can detect 10-250 µU ALP in samples.

Bacterial Alkaline Phosphatase (BAP) is a phosphomonoesterase that hydrolyzes 3’ and 5’ phosphates from DNA and RNA. It is suitable for removing 5, phosphates prior to end-labeling and for dephosphorylating vectors prior to insert ligation. BAP is active at 65°C for at least 1 h. It is sensitive to inhibition by micromolar amounts of inorganic phosphate. The enzyme is puri�ed from E. coli antibody HRP conjugate .Horse-radish Peroxidase is coupled to IgG antibody in a two-step procedure. In the �rst step monosaccharide residues in the enzyme are oxidized with periodate to produce aldehyde groups. Then, in the second step, the aldehyde groups are allowed to react with amino groups in the IgG antibody. The Schiff bases formed are reduced and the conjugate is puri�ed by dialysis.

Pro TechEx-Bacterial Alkaline Phosphatase Activity Teaching Kit

Cellulose acetate is an acetate salt of cellulose produced by treating cotton with acetic acid using sulphuric acid as a catalyst. Migration takes place on the buffer �lm on the surface of the cellulose acetate plate or membrane. Separation of the proteins is primarily by charge. Cellulose acetate electrophoresis may be used for qualitative identi�cation of variants, but also with elution for quantitation of the haemoglobins, A2, A, S, D, Lepore, α-chain variants, Hb H and Hb Bart's.

Pro TechEx-Cellulose Acetate Electrophoresis Teaching Kit


Pro TechEx-Antibody HRP Conjugation Teaching Kit 5 Exps 6100.00OP-3204-5xp


Pro TechEx-Cellulose Acetate Electrophoresis Teaching Kit 10 Exps 2700.00OP-3206-10xp


Pro TechEx-Bacterial Alkaline Phosphatase Activity Teaching Kit 5 Exps

10 Exps

3100.00

5400.00

OP-3205-5xp

OP-3205-10xp Pro TechEx-Bacterial Alkaline Phosphatase Activity Teaching Kit

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Expression of a recombinant protein can be approached in general by constructing a plasmid that encodes the desired protein, introducing the plasmid into the required host cell, growing the host cells and inducing protein expression, and then lysing the cells, purifying the protein, and performing SDS-PAGE analysis to verify the presence of the protein .Protein expression can be induced by a rising of the incubation temperature or by the addition of an inducing chemical such as isopropyl-b-D-thiogalactoside (IPTG) to the culture medium.

Pro TechEx-Clone Gene Analysis by SDS - PAGE Teaching Kit

Lactate dehydrogenase (L-lactate:NAD oxidoreductase (LDH) is a tetrameric protein catalyzing the reversible conversion of pyruvate to lactate. The enzyme uses NAD /NADH as cofactor and exists in six isoforms, �ve depending on the combination of the two subunits A (muscle) and B (heart), each with a molecular mass of 36 kDa and an additional homotetrameric LDH-C (testis)-isoform.

Pro Tech Ex- Enzyme Isolation from Bacteria Teaching Kit

Enzyme kinetics is the study of the chemical reactions that are catalysed by enzymes. In enzyme kinetics, the reaction rate is measured and the effects of varying the conditions of the reaction are investigated.

Pro TechEx-Enzyme Kinetics Teaching Kit

1. A new method has been developed for the speci�c extraction of histone fraction f2 (a) from calf thymus deoxyribonucleoprotein at pH7 by using a mixture of ethanol and guanidinium chloride.2. Fraction f2 (a) has been separated into the sub fractions f2(a)1 and f2(a)2 by acetone precipitation from acid solution, and at pH7. 3. Modi�cations of existing electrophoretic methods are described that enable these fractions to be more easily characterized

Pro TechEx-Histone Isolation From Nuclei Teaching Kit

COMPONENTS

Ampicillin (4°C)Inducer (IPTG)(4°C)LB broth powder(RT)Non Recombinant bacterial culture (4°C)Recombinant bacterial culture (4°C)Separating gel mix(4°C)TEMED(4°C)

APS (ammonium per sulphate)(4°C)Destainer(RT)Running gel buffer mix (powder) (RT)Sample solubilizing buffer(4°C)Stacking gel mix(4°C)Stainer (RT)Protocol

COMPONENTS

20XPBS (RT)Ammonium Sulphate (RT)APS (RT)Extraction buffer (4°C)SDS 10% (RT)Separating gel mix (4°C)Stacking gel mix (4°C)TEMED (RT)

Running buffer (RT)Control Protein (4°C)Destainer (RT)Dialysis membrane with Rubber (RT)Sample solublising buffer (RT)Stainer (RT)Protocol

COMPONENTS

Reaction buffer (RT)Reaction buffer pH 6.0 (RT)Reaction buffer pH 7.0 (RT)Reaction buffer pH 8.0 (RT)Reaction buffer pH 9.0 (RT)Reaction buffer pH 10.0 (RT)

Substrate (4°C)Substrate * (4°C)Inhibitor(NaOH) (RT)Enzyme (-20°C)Protocol


Pro TechEx-Clone Gene Analysis by SDS - PAGE Teaching Kit 5 Exps 5300.00OP-3207-5xp


Pro Tech Ex- Enzyme Isolation from Bacteria Teaching Kit 5 Exps 3717.00OP-3208-5xp


Pro TechEx-Enzyme Kinetics Teaching Kit 5 Exps 3700.00OP-3209-5xp

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COMPONENTS

Chromatin Sample (4°C)Denaturation buffer (4°C)Ethanol (RT)Extraction buffer (4°C)Solubilizing Buffer (RT)SSC Buffer (4°C)APS(4°C)

Running buffer (RT)Sample solublizing buffer(4°C)SDS 10% (RT)Separating gel mix (4°C)Stacking gel mix(4°C)TEMED(4°C)Destainer (RT)

Protein I(4°C)Protein II(4°C)Protein III(4°C)Protein Marker(4°C)Stainer(RT)Protocol

COMPONENTS

Precipitation solution I (RT)Precipitation solution II (RT)Phosphate buffer saline (RT)Syringe (RT)Sample solublising buffer(4°C)Protocol

APS(4°C)Running buffer (RT)SDS 10% (RT)Separating gel mix (4°C)Stacking gel mix(4°C)TEMED(4°C)

Destainer (RT)Protein I(4°C)Protein II(4°C)Protein III(4°C)Protein Marker(4°C)Stainer(RT)


Pro TechEx-Histone Isolation From Nuclei Teaching Kit 5 Exps 3700.00OP-3210-5xp

COMPONENTS

4X Separating gel buffer (4°C)4X Stacking gel buffer (4°C)Acrylamide monomer (4°C)Equilibrium Buffer (4°C)Gel Monomer Solution (4°C)Protein Sample (4°C)Sample Overlay Solution(4°C)Protocol

Ampholytes (4°C)APS (4°C)IEF Sample Buffer (4°C)NaOH (RT)Phosphoric acid (RT)TEMED (4°C)Urea (RT)20% Nonidet P-40 (NP–40) (4°C)

Destainer (RT)Glycine (RT)Para�lm (RT)SDS (RT)Stainer (RT)Syringe (RT)Tris (RT)


Pro TechEx-Isoelectrofocusing and 2-D Gel Electrophoresis (Tube Gel ) Teaching Kit 10 Exps 6100.00OP-3213-10xp


Pro TechEx-Immunohistochemistry Teaching Kit 5 Exps 6200.00OP-3212-5xp

The method involves two important steps. The �rst one is the removal of lipids and the second is the precipitation of total IgY from the supernatant of step one. After dialysis against a buffer (normally PBS) the IgY-extract can be stored at -20°C for more than a year. The purity of the extract is around 80%, the total IgY per egg varies from 40-80 mg.

Pro TechEx-IgY Puri�cation and analysis by SDS -PAGE Teaching Kit

Immunohistochemistry is the localization of antigens or proteins in tissue sections by the use of labeled antibodies as speci�c reagents through antigen-antibody interactions that are visualized by a marker such as enzyme.

Pro TechEx-Immunohistochemistry Teaching Kit

Isoelectric focusing methods are widely applied for the separation of proteins, peptides and enzymes. In a pH gradient the sample compo-nents migrate towards the anode or the cathode to the pH values, where their net charges are zero is called isoelectric point (pI).

Pro TechEx-Isoelectrofocusing and 2-D Gel Electrophoresis (Tube Gel ) Teaching Kit


Pro TechEx-IgY Puri�cation and analysis by SDS -PAGE Teaching Kit 5 Exps

10 Exps

2930.00

5100.00

OP-3211-5xp

OP-3211-10xp Pro TechEx-IgY Puri�cation and analysis by SDS -PAGE Teaching Kit

COMPONENTS

Primary antibody (-20°C)Secondary antibody HRP conjugate (-20°C)Blocking Solution (-20°C)DAB Reagent (-20°C)Ethanol 100% (RT)Hematoxylin (RT)Solution I (RT)Solution II (RT)

Hydrogen Peroxide (RT)Paraf�n tissue slide (+ve) (RT)Paraf�n tissue slide (–ve) (RT)Wash buffer (RT)DPX mountant (RT)10X PBS (RT)Coverslips(RT)Protocol

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Native PAGE uses the same discontinuous chloride and glycine ion fronts as SDS-PAGE to form moving boundaries that stack and then separate polypeptides by charge to mass ratio. Proteins are prepared in a non-reducing non-denaturing sample buffer, which maintains the protein’s secondary structure and native charge density. Therefore you can easily see multiple bands from the camshot of your native PAGE gel if your target protein has polymerized forms in your sample. In native PAGE electrophoresis most proteins have an acidic or slightly basic pl (isoelectric point) (~3–8) and migrate towards the negative polar.


Pro TechEx-NATIVE - PAGE Teaching Kit 10 Exps

20 Exps

3500.00

6200.00

OP-3214-10xp

OP-3214-20xp Pro TechEx-NATIVE - PAGE Teaching Kit

Pro TechEx-NATIVE - PAGE Teaching Kit

This method utilizes a combination of DNase and DNA Polymerase to nick one strand of the DNA helix, then incorporate labeled nucleotides as the polymerase examines, or “proofreads” the nicked site. Large plasmids, cosmids, and PCR fragments are all regularly used with this method, and the DNA can be linearized or supercoiled. Individual templates produce consistent results in the standard 90-minutes reaction, and result in an average probe length of 200 bp up to 500 bp.

Pro TechEx-Preparation of Biotinylated Probes by Nick Translation Teaching Kit

Protein electrophoresis in agarose gel is an alternative approach to using polyacrylamide gels and provides several bene�ts. Gels can be run using a vertical system or a horizontal system and unlike polyacrylamide gels,agarose gels can be used effectively to separate proteins larger than 600,000 Da.

Pro TechEx-Protein Agarose Gel Electrophoresis Teaching Kit

This experiment is designed to teach the students how the protein is degraded by protease enzyme.This kit can be used to study,1. How Protein fragments are degraded,2. Study the action of protease in degradation of proteins into peptides.3. Protein electrophoresis is used to demonstrate the effect of protease on protein.

Pro TechEx-Protein Degradation Teaching Kit

COMPONENTS

APS(4°C)Running buffer (RT)Sample solublising buffer(4°C)Separating gel mix (4°C)Stacking gel mix(4°C)TEMED(4°C)Destainer (RT)

Protein I(4°C)Protein II(4°C)Protein III(4°C)Protein Marker(4°C)Stainer(RT)Protocol

COMPONENTS

2X Destainer (RT)5X Running Buffer (RT)Agarose(RT)Protein Sample(4°C)Sample Buffer(4°C)Stainer(RT)Protocol


Pro TechEx-Preparation of Biotinylated Probes by Nick Translation Teaching Kit 5 Exps 5900.00OP-3215-5xp


Pro TechEx-Protein Agarose Gel Electrophoresis Teaching Kit 10 Exps 2800.00OP-3216-10xp

COMPONENTS

Biotin-16-dUTP (-20°C)Bovine serum albumin (BSA) (-20°C)Dig-11-dUTP (-20°C)DNase I from bovine pancreas (-20°C)dNTP Mix (-20°C) 0.5 M EDTA (RT)

2M Magnesium chloride(RT)b-Mercaptoethanol, 99%(RT)Glycerol (RT)Lambda HindIII marker (-20°C)Polymerase (-20°C)1 M NaCl (RT)

1M Tris-HCl (RT)Sterile Water (RT)Protocol

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Pro TechEx-Protein Degradation Teaching Kit 5 Exps 2600.00OP-3217-5xp

COMPONENTS

Acrylamide (4°C)APS (4°C)Running Gel Buffer (RT)SDS (RT)Separating buffer (RT) Stacking buffer(RT) TEMED (4°C)

70% Isopropanol(RT)Microfuge tubes (RT)Protein Sample(-20°C)Proteinase K(-20°C)Sterile water (RT)Stop Solution (RT)Protocol


Pro TechEx-Protein Estimation Teaching Kit 3 x 5 Exps 2700.00OP-3218-5xp

COMPONENTS

Protein sample (BSA) (4°C)Unknown sample(4°C)Reagent A (RT)Reagent B (RT)Reagent C (RT)

Bradford reagent (4°C)Biuret reagent (RT)Folin reagent (4°C)Protocol

COMPONENTS

Separating buffer (RT)Stacking buffer(RT)Running Gel Buffer (RT)SDS (RT)Acrylamide (4°C)Sample solubilizing buffer (RT)Protocol

APS (RT)70% Isopropanol (RT)TEMED (4°C)Protein Lysate (4°C)Protein Marker (4°C)Microfuge tubes (RT)

Protein Estimation Teaching Kit (Quantitative) enables the determination of the concentration of unknown proteins based on various quantita-tive tests. The Kit is designed for rapid and accurate determination of proteins by three most commonly used techniques, each having features that suit it to a particular use. It enables the determination of the concentration of proteins which is frequently required in biochemical work. Most protein assays take advantage of a reaction between a reagent dye and the protein of interest that will shift or increase the absorbance of a particular wavelength. The choice of the method depends on the nature of the protein, the nature of the other components in the protein sample, desired speed, accuracy and sensitivity of the assay.

Pro TechEx-Protein Estimation Teaching Kit

Protein folding is the process by which a protein structure assumes its functional shape or conformation. It is the physical process by which a polypeptide folds into its characteristic and functional three-dimensional structure from random coil.

Pro TechEx-Protein Folding Teaching Kit


Pro TechEx-Protein Folding Teaching Kit 5 Exps

15 Exps

2700.00

6000.00

OP-3219-5xp

OP-3219-15xp Pro TechEx-Protein Folding Teaching Kit

SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis, is a technique used in biochemistry, genetics and molecular biology to separate proteins according to their molecular weight. The electrophoretic mobility of proteins depends upon their size. As proteins are amphoteric compounds, their net charge can therefore be determined by the pH of the medium in which they are suspended. Therefore, at a given pH and under non-denaturing conditions, the electrophoretic separation of proteins is determined by both size and charge of molecules. As proteins are high molecular weight molecules, it needs porous gels to get separated. Polyacrylamide gels are those which provide a means of separating proteins by size as they are porous.

Pro TechEx-SDS - PAGE Teaching Kit

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Pro TechEx-SDS- PAGE Teaching Kit 10 Exps

20 Exps

3500.00

6200.00

OP-3220-10xp

OP-3220-20xp Pro TechEx-SDS- PAGE Teaching Kit

COMPONENTS

APS(4°C)Running buffer (RT)Sample solublising buffer(4°C)SDS 10% (RT)Separating gel mix (4°C)Stacking gel mix(4°C)TEMED(4°C)

Destainer (RT)Protein I(4°C)Protein II(4°C)Protein III(4°C)Protein Marker(4°C)Stainer(RT)Protocol


Pro TechEx- Serum Protein Electrophoresis Teaching Kit 10 Exps 2900.00OP-3221-10xp


Pro TechEx-Silver Staining Teaching Kit 10 Exps 3900.00OP-3222-10xp

The serum protein electrophoresis (SPEP) test measures speci�c proteins in the blood to help identify some diseases. Proteins are substanc-es made up of smaller building blocks called amino acids. Proteins carry a positive or a negative electrical charge, and they move in �uid when placed in an electrical �eld. Serum protein electrophoresis uses an electrical �eld to separate the proteins in the blood serum into groups of similar size, shape, and charge.

Blood serum contains two major protein groups: albumin and globulin. Both albumin and globulin carry substances through the bloodstream. Using protein electrophoresis, these two groups can be separated into �ve smaller groups (fractions)

Pro TechEx- Serum Protein Electrophoresis Teaching Kit

Silver staining is used to detect proteins after electrophoretic separation on polyacrylamide gels. It combines excellent sensitivity (in the low nanogram range) . The rationale of silver staining is quite simple. Proteins bind silver ions, which can be reduced under appropriate conditions to build up a visible image made of �nely divided silver metal.

Basic steps involved in silver stainingi) Fixation to get rid of interfering compounds.ii) Sensitization and rinses to increase the sensitivity and contrast of the staining.iii) Silver impregnation with either a silver nitrate solution or a silver-ammonia complex solution.iv) Rinses and development to build up the silver metal image v) stop and rinse to end development prior to excessive background formation and to remove excess silver ion and other chemicals prior to further processing.

Pro TechEx-Silver Staining Teaching Kit

Calf intestinal alkaline phosphatase (CIAP) is a phosphomonoesterase puri�ed from calf intestinal mucosa that hydrolyzes 5'-phosphate groups from DNA, RNA, and nucleotides. CIAP is used to dephosphorylate linearized vector DNA prior to insert ligation and to remove 5'-phos-phate groups prior to 5'-end labeling of nucleic acids with T4 polynucleotide kinase.

Pro TechEx-Study of Calf Intestine Alkaline Phosphatase Activity (Dephosporylation of DNA) Teaching Kit

COMPONENTS

Agarose (RT)Electrophoresis buffer (RT)Sample buffer (4°C)Protein sample (4°C)Ponceau’s stain (RT)Protocol

COMPONENTS

Cross linking Solution 5X (RT)Developing Solution 5X (RT)Fixing Solution 5X (RT)

Formaldehyde (RT)Protein Sample (4°C)Reducing Agent 5X (RT)

Sample Solublizing Buffer (4°C)Silver Nitrate Solution 5X (RT)Protocol

charged R-groups

hydrophobic areas

BEFORE SDS

AFTER SDS

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Pro TechEx-Study of Calf Intestine Alkaline Phosphatase Activity( Dephosporylation of DNA) Teaching Kit 5 Exps 5400.00OP-3223-5xp

COMPONENTS

3M Sodium Acetate (RT)Calf Intestine Alkaline Phosphatase Solution(-20°C)Chloroform:Isoamyl Alcohol(24:1) (4°C)DNA(-20°C)Enzyme buffer (-20°C)Restriction Enzyme (-20°C)TE Buffer(RT)Tris saturated phenol (4°C)Protocol

COMPONENTS

Buffer A (4°C)S1 (4°C)S2 (4°C)S3 (4°C)RIPA buffer (4°C)Protocol


Pro TechEx-Study of cellular Fractionation Teaching Kit 5 Exps 6800.00OP-3224-5xp

This protocol has been adapted to fractionate a variety of different cell types into cytoplasm, nucleoplasm and nucleoli. It uses a hypotonic buffer to swell the cells prior to dounce homogenization to break them open.

Pro TechEx-Study of cellular Fractionation Teaching Kit

The experiment in this kit demonstrates the effect of an acid, salt, organic solubility of provided protein samples and how an anionic detergent has the effect of these parameters on protein precipitation.

The results shall encourage students to seek answers as to how proteins in aqueous solution react with acid, alkali, salt, detergent and heat. How these factors affect protein structure in solubility.

The results should help students to understand how altered physical properties can be exploited for protein Solubilization, puri�cation and characterization studies.

Pro TechEx-Study of Physical Properties of Protein Teaching Kit

Study of protein composition of various organs of an organism shall provide insight into the extent of protein diversity. The electrophoretic separation of proteins on a SDS-PAGE results in Protein �nger printing.

The objective of this Kit is: Familiarize students with diversity of Proteins from different organs of the same organism.1. Make students understand the diversity of proteins leads to diversity of organ functioning.2. Identi�cation of sample by Protein �nger printing (SDS - PAGE).

Pro TechEx-Study of Protein Fingerprinting Teaching Kit


Pro TechEx-Study of Physical Properties of Protein Teaching Kit 5 Exps

15 Exps

2400.00

5900.00

OP-3225-5xp

OP-3225-15xp Pro TechEx-Study of Physical Properties of Protein Teaching Kit

COMPONENTS

Acid precipitant (RT)Protein solution A (4°C)Protein solution B (4°C)Protein solution C (4°C)Tissue extraction buffer (-20°C)

1.5ml Microfuge tubes (RT)Detergent solution (RT)Organic Precipitant (RT)Salt solution (RT)Protocol

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Pro TechEx-Study of Protein Fingerprinting Teaching Kit 5 Exps

15 Exps

2800.00

6300.00

OP-3226-5xp

OP-3226-15xp Pro TechEx-Study of Protein Fingerprinting Teaching Kit


Pro TechEx-Western Blotting Teaching Kit 5 Exps

20 Exps

5100.00

15900.00

OP-3227-5xp

OP-3227-20xp Pro TechEx-Western Blotting Teaching Kit

COMPONENTS

10%SDS(RT)Destainer(RT)Microfuge tubes(RT)Running gel buffer(RT)Separating buffer (RT)Stacking buffer(RT)Stainer(RT)Protocol

40%Acrylamide(4˚C)APS (0.1g/ml)(4˚C)Protein Extract-Heart (-20˚C)Protein Extract-Kidney (-20˚C)Protein Extract-Liver(-20˚C)Protein Extract-Lung (-20˚C)Sample solubilizing buffer (4˚C)TEMED (4˚C)

COMPONENTS

APS (4°C)Running buffer (RT)SDS Sample buffer (4°C)Separating gel mix (4°C)Stacking gel mix (4°C)TEMED (4°C)Transfer buffer (RT)

10X PBST (RT)DAB (-20°C)Destainer(RT)Nitrocellulose membrane (RT)Primary antibody (-20°C)Secondary antibody HRP-conjugate (-20°C)Stainer (RT)

10% SDS (RT)Blocker (4°C)H

2O

2(4°C)

Methanol (RT)Protein sample(-20°C)Substrate buffer (4°C)Protocol

Western blotting is an analytical method wherein a protein sample is electrophoresed on an SDS-PAGE and electrotransferred onto nitrocel-lulose membrane. The transferred protein is detected using speci�c primary antibody and secondary enzyme labeled antibody and substrate. A protein sample is subjected to polyacrylamide gel electrophoresis. After this the gel is placed over a sheet of nitrocellulose and the protein in the gel is electrophoretically transferred to the nitrocellulose. The nitrocellulose is then incubated with the speci�c antibody for the protein of interest. The nitrocellulose is then incubated with a second antibody, which is speci�c for the �rst antibody.

Pro TechEx-Western Blotting Teaching Kit

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COMPONENTS

20X Diluent buffer (RT)6% Orcinol Reagent (RT)Ferric chloride, anhydrous (RT)Standard RNA(1mg/ml) (-20°C)Test RNA Solution (-20°C)Protocol


Pro TechEx-Estimation of RNA by Orcinol Method Teaching Kit 5 Exps 2200.00OP-3301-5xp


Pro TechEx-Invitro transcription Teahing Kit 5 Exps 6800.00OP-3302-5xp


Pro TechEx-Northern Blotting Teaching Kit 5 Exps 7100.00OP-3303-5xp

This is a general reaction for pentoses and depends on the formation of furfural when the pentose is heated with concentrated hydrochloric acid. Orcinol reacts with the furfural in the presence of ferric chloride as a catalyst to give a green colour, which can be measured at 665 nm.

Pro TechEx-Estimation of RNA by Orcinol Method Teaching Kit

In vitro transcription is a simple procedure that allows for template-directed synthesis of RNA molecules of any sequence from short oligonu-cleotides to those of several kilobases in μg to mg quantities. It is based on the engineering of a template that includes a bacteriophage promot-er sequence (e.g. from the T7 coliphage) upstream of the sequence of interest followed by transcription using the corresponding RNA polymer-ase. In vitro transcripts are used in analytical techniques .

Pro TechEx-Invitro transcription Teahing Kit

The term 'northern blot' actually refers speci�cally to the capillary transfer of RNA from the electrophoresis gel to the blotting membrane. However, the entire process is commonly referred to as northern blotting. The northern blot technique was developed in 1977 by James Alwine, David Kemp, and George Stark at Stanford University. Northern blotting takes its name from its similarity to the �rst blotting technique, the Southern blot, named for biologist Edwin Southern. The major difference is that RNA, rather than DNA, is analyzed in the northern blot.

Pro TechEx-Northern Blotting Teaching Kit

Northern hybridization is a widely used technique in molecular biology to determine the molecular weight of mRNA and to measure relative amounts of mRNA present in different samples and for identifying alternatively spliced transcripts and multigene family members. Northern Blotting involves separation of RNA samples according to size by agarose gel electrophoresis and detection with hybridization probe comple-mentary to part of or the entire target sequence. Northern Blot refers to capillary transfer of RNA from the electrophoresis gel to the blotting membranes.

Pro TechEx-Northern Hybridization Teaching Kit

COMPONENTS

DEPC treated water (-20°C)Murine RNase Inhibitor (-20°C)Ribonucleotide Solution Mix (-20°C) SP6 RNA Polymerase (-20°C)T7 RNA Polymerase Buffer (10X)(-20°C)Template DNA (-20°C)

1.5ml Microfuge Tubes (RT)70% Ethanol (RT)Gel loading dye (RT)Isopropanol (RT)RNase H(-20°C)Sodium acetate (3M)(RT)

Agarose (RT)Ethidium Bormide (RT)Phenol (RT)T7 RNA Polymerase (-20°C)TEB 10X (RT)Protocol

COMPONENTS

10X Running buffer (RT)Agarose (RT)Formaldehyde (RT)RNA Sample buffer (RT)

20X SSC (RT)Gel loading dye (RT)RNA Sample (-20°C)Protocol

TRANSCRIPTOMICS

Gel Filter

RNAtransferred

to filter

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COMPONENTS

Agarose (RT)Ethidium Bromide (RT)Formaldehyde(RT)Gel Loading dye (RT)MOPS 10 X (RT)RNA sample (-20°C)Transfer buffer (RT)Transfer membrane (RT)

Blocker (4°C)Conjugate (4°C)Prehybridization Buffer (4°C)Probe (biotinylated) (-20°C)Substrate (4°C)Wash Buffer (RT)Protocol

COMPONENTS

10X MOPS buffer (RT)Agarose (RT)Chloroform Solution (4°C)Ethidium Bromide (RT)Extraction buffer (4°C)Gel loadingDye (RT)Phenol:Chloroform Solution (4°C)

Sodium acetate pH 5.2 (RT)70% ethanol (RT)Absolute ethanol (4°C)DEPC-treated water (RT)Formaldehyde (RT)Marker (RT)Protocol

COMPONENTS

10X MOPS (-20°C)Binding buffer (RT)Elution buffer (RT)Ethanol (RT)Oligo dT Cellulose (-20°C)Sodium acetate (RT)Total RNA (-20°C)

70% ethanol (RT)Agarose (RT)DEPC water (RT)Ethidium Bromide (RT)Formaldehyde (RT) Gel loading Dye (RT)Protocol


Pro TechEx-Northern Hybridization Teaching Kit 5 Exps 9300.00OP-3304-5xp


Pro TechEx-Total RNA Extraction and RT-PCR Teaching Kit 10 Exps 10100.00OP-3307-10xp

Isolation of pure, under graded total RNA from plant tissue can be dif�cult because of high levels of ribonucleases, pectins and polyphenols that are present in plant material. Another major problem is the presence and removal of proteins. This problem is commonly solved by lysing the plant cells in the presence of phenol/chloroform.

Pro TechEx-Plant RNA Isolation Teaching Kit


Pro TechEx - Plant RNA Isolation Teaching Kit 5 Exps

10 Exps

3300.00

5800.00

OP-3305-5xp

OP-3305-10xp Pro TechEx - Plant RNA Isolation Teaching Kit

To isolate mRNA from total RNA sample, cellulose resin with a poly deoxythimidine oligomer attached (oligo-dT cellulose) is used to purify transcript with a poly-A tail. This puri�cation should be done on the same day as the total RNA puri�cation limit degradation. The total RNA and oligo-dT cellulose are incubated at 65°C and eluted by using elution buffer. Puri�ed mRNA can be used for Reverse transcription process.

Pro TechEx-Preparation of Poly(A) mRNA From Yeast Teaching Kit

Reverse transcription polymerase chain reaction (RT-PCR) is one of many variants of polymerase chain reaction (PCR). This technique is commonly used in molecular biology to detect RNA expression. In RT-PCR, the RNA template is �rst converted into a complementary DNA (cDNA) using a reverse transcriptase. The cDNA is then used as a template for exponential ampli�cation using PCR. RT-PCR is currently the most sensitive method of RNA detection available.RT-PCR is used to qualitatively detect gene expression through creation of complementary DNA (cDNA) transcripts from RNA.

Pro TechEx-Total RNA Extraction and RT-PCR Teaching Kit


Pro TechEx-Preparation of Poly(A) mRNA From Yeast Teaching Kit 5 Exps

10 Exps

4800.00

7700.00

OP-3306-5xp

OP-3306-10xp Pro TechEx-Preparation of Poly(A) mRNA From Yeast Teaching Kit

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COMPONENTS

1.5ml Microfuge tubes (RT)10mM dNTP (-20°C)10X MOPS (RT)Agarose (RT)Ethidium Bromide (RT)Gel Loading dye (RT)Primer F (-20°C)Yeast Culture (4°C)

0.2ml PCR tubes (RT)Primer R (-20°C)RNase free Water (4°C)Solution I (4°C)Solution II (4°C)Solution III (4°C)Solution IV (4°C)Yeast broth (RT)

DNA Marker (-20°C)M-MuLV RT Buffer 5X (-20°C)Random primer (-20°C)RNase inhibitor (-20°C)Taq polymerase (-20°C)Taq polymerase Buffer 10X (-20°C)Enzyme (-20°C)Protocol

Mid-exponential phase bacteria grown in liquid media (50–100µl pellet volume) were harvested by centrifugation at 13500 rpm for 20s in a Microfuge tube, washed in detergent solution. Repelleted and resuspended in RNase free distilled water. Re-extracted with water saturated phenol. Isolated RNA was visualized by Ethidium Bromide Stained Denaturing Formaldehyde gel electrophoresis.

Pro TechEx-Total RNA Extraction from Bacteria Teaching Kit


Pro TechEx-Total RNA Extraction from Bacteria Teaching Kit 10 Exps

20 Exps

3800.00

6500.00

OP-3308-10xp

OP-3308-20xp Pro TechEx-Total RNA Extraction from Bacteria Teaching Kit

This protocol uses Trizol (also known as TRI REAGENT) for the isolation of total RNA. Trizol is a mixture of guanidine thioacyanate and phenol, which effectively dissolves DNA, RNA and protein on homogenization or lysis of tissue sample. After adding chloroform and centrifuging, the mixture separates into 3 phases with the upper clear aqueous phase containing the RNA. The next steps in the extraction are washes and precipitation of the RNA.

Pro TechEx-Total RNA isolation from Yeast by Trizol method Teaching Kit

RNA isolation is dif�cult procedure .Get rid of RNases is an essential step with the help of DEPC water. The objective is to isolate a large quantity (perhaps 100 whole micrograms) of high molecular weight, undegraded total RNA. In order to further characterize the mating reaction will be isolating total RNA from culture contains yeast cells in logarithmic growth phase grown in YPD.

Pro TechEx-Total RNA Extraction From Yeast Teaching Kit


Pro TechEx-Total RNA isolation from Yeast by Trizol method Teaching Kit 5 Exps

10 Exps

4800.00

8100.00

OP-3309-5xp

OP-3309-10xp Pro TechEx-Total RNA isolation from Yeast by Trizol method Teaching Kit


Pro TechEx-Total RNA Extraction From Yeast Teaching Kit 5 Exps

10 Exps

3500.00

6200.00

OP-3310-5xp

OP-3310-10xp Pro TechEx-Total RNA Extraction From Yeast Teaching Kit

COMPONENTS

Microfuge tubes (RT)10X MOPS (RT)70% Ethanol (RT)Agarose (RT)E.coli Host (4°C)Ethidium bromide (RT)Gel loading dye (RT)Agarose (RT)

DEPC water (RT)LB medium (RT)Solution I (4°C)Solution II (4°C)Solution III (4°C)Solution IV (4°C)Protocol

COMPONENTS

Chloroform (RT)TE buffer (RT)Trizol (4°C)Wash solution (RT)Yeast culture (4°C)10X MOPS (RT)

Agarose (RT)Ethidium bromide (RT)Formaldehyde (RT)Gel loading dye (RT)Protocol

COMPONENTS

Ethanol I (RT)Ethanol II (RT)Isopropanol (RT)Phenol/chloroform (4°C)

RNA buffer (RT)RNA buffer +SDS (RT)Yeast culture (4°C)YPD broth (RT)

10X MOPS (RT)Agarose (RT)Ethidium bromide (RT)Formaldehyde (RT)

Gel loading dye (RT)TE buffer (RT)Protocol

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Test is based on the heamagglutination reaction. Human RBCs possessing A or B antigen will agglutinate with the corresponding anti-body. Agglutination of RBCs with Anti-A monoclonal, Anti-B monoclonal or Anti-A, B monoclonal is a positive test result and indicates the presence of the corresponding Antigen. Absence of agglutination of RBCs with Anti-A monoclonal, Anti-B monoclonal or Anti-AB monoclonal is a negative test result and indicates the absence of the corresponding antigen. Anti-B is especially useful in detecting the weak variance of A and B which are occasionally missed by the routinely used monoclonal Anti-A and Anti-B Anti-sera.

Pro TechEx - ABO Blood Grouping With Rh-Factor Teaching Kit

ASO latex test is based on principle of agglutination. When latex reagent (Polystyrene Latex particles coated with Streptolysin O) is mixed with a test serum containing antibodies to streptolysin-O higher than 200IU/ml gives visible agglutination reaction. If the concentration of ASO is lesser than 200IU/ml, no agglutination will be observed.

Pro TechEx - ASO Latex Agglutination Teaching Kit

Complement �xation referred as the binding and activation of complement to Ag-Ab complex. Normally complements bind to Ag-Ab complex, i.e. antigen-antibody reactions lead to immune complex formation which produces complement �xation via the classical pathway and this may be exploited to determine the amount of antigen or antibody present. Complement �xation can detect antibody level of less than one microgram per ml.

Pro TechEx - Complement Fixation Test Teaching Kit

CRP latex test is based on principle of agglutination. When latex reagent (polystyrene Latex particles coated with anti CRP) is mixed with a test serum containing antibodies to CRP higher than 0.8mg/dl gives visible agglutination reaction. If the concentration of CRP is lesser than 0.8mg/dl, no agglutination will be observed.

Pro TechEx - C-Reactive Protein (CRP) Latex Teaching Kit

COMPONENTS

Anti-A (4°C)Anti-B (4°C)Anti-D (4°C)Ethanol (RT)

Applicator sticks (RT)Glass Slides (RT)Lancetts (RT)Protocol

COMPONENTS

ASO latex Reagent (4°C)ASO positive control (4°C) ASO Negative control (4°C)Disposable mixing sticks (RT)Test slide (RT)Protocol

COMPONENTS

Buffer (4°C)Complement with antigen (4°C)Negative serum (4°C)Positive serum (4°C)Protocol

IMMUNOLOGY


Pro TechEx - ABO Blood Grouping With Rh-Factor Teaching Kit 20 Exps 1450.00OP-3401-20xp


Pro TechEx - ASO Latex Agglutination Teaching Kit 20 Exps 1200.00OP-3402-20xp


Pro TechEx - Complement Fixation Test Teaching Kit 5 Exps 5700.00OP-3403-5xp

AGGLUTINATION TECHNIQUES

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Pro TechEx - C-Reactive Protein (CRP) Latex Teaching Kit 20 Exps 1200.00OP-3404-20xp


Pro TechEx - Latex Agglutination Teaching Kit 10 Exps 2400.00OP-3406-10xp


Pro TechEx - RA (Rheumatoid Arthritis) Test Teaching Kit 20 Exps 1100.00OP-3407-20xp

COMPONENTS

CRP latex Reagent (4°C)CRP Positive control (4°C)CRP Negative control (4°C)Disposable mixing sticks (RT)Test slide (RT)Protocol

COMPONENTS

10X PBS (RT)Alsever’s solution (4°C)ELISA Module (RT)RBC storage solution (4°C)Viral sample (4°C)Protocol

COMPONENTS

Antigen (-20°C)Disposable Mixing Sticks (RT)Negative control (-20°C)Positive control (-20°C)Template (RT)Protocol

The Hemagglutination assay is a method for tittering in�uenza viruses based on their ability to attach to molecules present on the surface of red blood cells. A viral suspension may agglutinate the red blood cells, thus preventing them from settling out of suspension. By serially diluting a virus in a 96-well plate and adding a consistent amount of red blood cells, an estimation of the amount of virus present can be made.

Pro TechEx - Haemagglutination Teaching Kit

Latex agglutination is observed when a sample containing the speci�c antigen (or antibody) is mixed with an antibody (or antigen) which is coated on the surface of latex particles.

Pro TechEx - Latex Agglutination Teaching Kit

Rheumatoid arthritis is a disease that affects the joints. It causes pain, swelling, and stiffness. If one knee or hand has rheumatoid arthritis, usually the other does too. This disease often occurs in more than one joint and can affect any joint in the body. People with this disease may feel sick and tired, and they sometimes get fevers.

Pro TechEx - RA (Rheumatoid Arthritis) Test Teaching Kit


Pro TechEx - Haemagglutination Teaching Kit 10 Exps 3500.00OP-3405-10xp

COMPONENTS

Antigen (-20°C)Antibody (-20°C)Latex Beads (4°C)Coating Buffer (4°C)PBS (RT)

Mixing sticks (RT)Microfuge tubes (RT)Test Slide (RT)Protocol

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The rapid plasma reagin (RPR) refers to a type of rapid diagnostic test that looks for non-speci�c antibodies in the blood of the patient that may indicate that the organism (Treponema pallidum) that causes syphilis is present. The term "reagin" means that this test does not look for antibodies against the actual bacterium, but rather for antibodies against substances released by cells when they are damaged by T. pallidum.

In addition to screening for syphilis, an RPR level (also called a "titer") can be used to track the progress of the disease over time and its response to therapy.

Pro TechEx - RPR Test Teaching kit

The venereal disease research laboratory (VDRL) test is designed to assess whether or not you have syphilis, a sexually transmitted infection. Instead of testing for the bacteria that causes syphilis, the VDRL test checks for antibodies to this bacteria. Immune system produces a speci�c kind of antibody (a type of protein) when it defends body from syphilis.

Pro TechEx - VDRL Test Teaching Kit

Enteric fever speci�c agglutinins (antibodies) are detected in patients after 15 days of fever. BCG vaccinated patient’s serum may show elevated titre of all three ‘H’ agglutinins. Stained salmonella antigens are used to detect and identify speci�c antibodies in serum samples from patients suffering from enteric fever.Bacterial suspension which carry antigen will agglutinate on exposure to antibodies to salmonella organ-isms.

Pro TechEx - Widal Slide Test Teaching Kit

Enteric fever occurs when pathogenic microorganisms like S. typhi, S. paratyphi A, S. paratyphi B infect the human body. During the course of disease, the body responds to this antigenic stimulus by producing antibodies whose titre rises slowly in early stages, to a maxima and then slowly falls till it is undetectable. Antibodies to salmonella organisms may be detected in the patient serum from the second week after onset of infection. Information regarding the titres and whether or not they are rising or falling can be obtained by performing serological tests using widal antigen suspensions. Usually tube titres of 1:80 and above are taken as diagnostically signi�cant, however for endemic areas higher cut-offs may need to be established .

Pro TechEx - Widal Tube Test Teaching Kit


Pro TechEx - RPR Test Teaching kit 20 Exps 1100.00OP-3408-20xp

COMPONENTS

Positive control serum (-20°C) Negative control serum (-20°C)RPR antigen (-20°C) Mixing stick (RT)RPR slide (RT)Protocol


Pro TechEx - VDRL Test Teaching Kit 10 Exps 1000.00OP-3409-10xp


Pro TechEx - Widal Slide Test Teaching Kit 20 Exps 1100.00OP-3410-20xp

COMPONENTS

Positive control serum (-20°C) Negative control serum (-20°C) VDRL antigen (-20°C) Mixing stick (RT)VDRL slide (RT)Protocol

COMPONENTS

Salmonella typhi ‘O’ (4°C)Salmonella typhi ‘H’ (4°C)Salmonella paratyphi ‘AH’ (4°C)Salmonella paratyphi ‘BH’ (4°C)Positive control serum (PC) (4°C)Negative control serum (NC) (4°C)Widal slide (RT)Applicator sticks (RT)Protocol

Figure 1 Figure 2

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Pro TechEx - Widal Tube Test Teaching Kit 10 Exps 2000.00OP-3411-10xp

COMPONENTSNormal Saline (RT)Salmonella paratyphi ‘AH’ (4°C)Salmonella paratyphi ‘BH’ (4°C)Salmonella typhi ‘H’ (4°C)Salmonella typhi ‘O’ (4°C)Test Serum (4°C)Protocol

CHROMATOGRAPHY

Af�nity chromatography is a method of separating biochemical mixtures based on a highly speci�c interaction such as that between antigen and antibody, enzyme and substrate, or receptor and ligand.

Pro TechEx-Af�nity Chromatography Teaching Kit

Column chromatography is one of the most useful methods for the separation and puri�cation of both solids and liquids. This is a solid - liquid technique in which the stationary phase is a solid & mobile phase is a liquid. The principle of column chromatography is based on differential adsorption of substance by the adsorbent.

Pro TechEx-Column Chromatography Teaching Kit


Pro TechEx-Af�nity Chromatography Teaching Kit 5 Exps 3570.00OP-3501-5xp


Pro TechEx-Column Chromatography Teaching Kit 10 Exps 3080.00OP-3502-10xp

Gel �ltration chromatography (sometimes referred to as molecular sieve chromatography) is a method that separates molecules according to their size and shape. The separation of the components in the sample mixture, with some exceptions, correlates with their molecular weights. In these cases, gel �ltration can be used as an analytical method to determine the molecular weight of an uncharacterized molecule. Gel �ltration is also an important preparative technique since it is often a chromatographic step in the puri�cation of proteins, polysaccharides and nucleic acids.

Pro TechEx-Gel Filtration Chromatography Teaching Kit


Pro TechEx-Gel Filtration Chromatography Teaching Kit 5 Exps 3525.00OP-3503-5xp

COMPONENTS

Anti Rabbit IgG (antibody) (-20°C)Elution buffer (4°C)Equilibration buffer (4°C)Protein A sepharose (4°C)Serum Sample (4°C)Washing buffer (RT)

Agarose (RT)Chromatography column (RT)Microfuge tubes (RT)Normal saline (RT)Protocol

COMPONENTS

Alumina (RT)Benzene (RT)Column (RT)Methanol (RT)Sodium sulphate (RT)Protocol

COMPONENTS

10X Equilibration Buffer (4°C)Column (4°C)Protein Mixture (-20°C)Protocol

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Af�nity chromatography is a particularly powerful procedure, which can be used to purify Immunoglobulin, sub populations of IgG, or the antigen binding fraction of IgG present in serum/ascitic �uid/hybridoma culture supernatent. This technique requires the production of the solid matrix to which a ligand having either af�nity for the revelent IgG or Vice Versa has been bound.

Pro TechEx-IgG Puri�cation Teaching Kit

IMAC is based on the interaction between molecules in solution and metal ions immobilized on a solid support. The molecules are separated according to their af�nity for the chelated metal ions, which depends on the coordination between the chelated metal ion and electron donor groups from the ligand. The ligands are assumed to bind to the metal through ligand exchange. Two characteristics of the metal–ligand bond can be used for the successful separation of different ligands.

Pro TechEx-Immobilized Metal Ion Af�nity Chromatography Teaching Kit

Af�nity chromatography is a particularly powerful procedure, which can be used to purify Immunoglobulin, sub populations of Ig, or the antigen binding fraction of Ig present in serum/ascitic �uid/hybridoma culture supernatent. This technique requires the production of the solid matrix to which a ligand having either af�nity for the revelent Immunoglobulin has been bound.

Pro TechEx-Immunoglobulin Puri�cation Teaching Kit

Ion Exchange chromatography is a powerful procedure, which can be used to purify IgG(antibody), sub populations of IgG, or the antigen binding fraction of IgG present in serum/ascitic �uid/hybridoma culture supernatent. This technique requires the production of the solid matrix (DEAE Cellulose)to which a ligand having either af�nity for the revelent IgG or Vice Versa has been bound.

Pro TechEx-Ion Exchange Chromatography (Antibody Puri�cation ) Teaching Kit


Pro TechEx-IgG Puri�cation Teaching Kit 5 Exps 4200.00OP-3504-5xp


Pro TechEx-Immobilized Metal Ion Af�nity Chromatography Teaching Kit 5 Exps 12500.00OP-3505-5xp

COMPONENTS

Anti Rabbit IgG (antibody) (4°C)Elution buffer (4°C)Equilibration buffer (4°C)Protein A sepharose (4°C)Serum Sample (4°C) Washing buffer (RT)

Agarose (RT)Chromatography column (RT)Normal saline (RT)Microfuge tubes (RT)Protocol

COMPONENTS

Crude enzyme sample (-20°C)DEAE cellulose (-20°C)Elution buffer (4°C)Equilibration buffer (4°C)Substrate (-20°C)Wash buffer (RT)

Column (RT)CuSo

4 solution (RT)

Microfuge tubes (RT)NaOH solution (RT)Reaction buffer (RT)Protocol


Pro TechEx-Immunoglobulin Puri�cation Teaching Kit 5 Exps 4780.00OP-3506-5xp

COMPONENTS

Anti Rabbit IgG (antibody) (4°C)Elution buffer (4°C)Equilibration buffer (4°C)Protein A sepharose (4°C)Serum Sample (4°C)Washing buffer (RT)

Agarose (RT) Chromatography column (RT)Normal saline (RT)Microfuge tubes (RT)Protocol


Pro TechEx-Ion Exchange Chromatography (Antibody Puri�cation ) Teaching Kit 5 Exps 3500.00OP-3507-5xp

COMPONENTS

10X Equlibration buffer (4°C)10X wash buffer (4°C)5X Elution buffer (4°C)DEAE cellulose (4°C)Anti bovine IgG (4°C)

Crude sample (4°C)Agarose (RT)Normal saline (RT)Column (RT)Protocol

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Ion exchange is probably the most frequently used chromatographic technique for the separation and puri�cation of proteins, polypeptides, nucleic acids, polynucleotides, and other charged biomolecules . The reasons for the success of ion exchange are its widespread applicability, its high resolving power, its high capacity, and the simplicity and controllability of the method.

Pro TechEx-Ion Exchange Chromatography (Lysozyme Puri�cation) Teaching Kit

Paper Chromatography is a separation technique that is used to separate and identify the components of a mixture . Paper chromatography is used to identify colouring agents.

Pro TechEx-Paper Chromatography Teaching Kit

Thin-layer chromatography or TLC, is a solid-liquid form of chromatography where the stationary phase is normally a polar absorbent and the mobile phase can be a single solvent or combination of solvents. TLC is a quick, inexpensive microscale technique that can be used to:

• Determine the number of components in a mixture• Verify a substance’s identity

Pro TechEx-Thin Layer Chromatography Teaching Kit

Antibody capture ELISA is a sophisticated, complex test that has proved useful in the diagnosis of many infectious diseases (e.g. rubella, measles, and toxoplasmosis). Antibody capture ELISA are particularly sensitive in demonstrating IgM responses early in illness. Reasons for this increase in sensitivity are: speci�c antibody isotypes in the patient's specimen are bound to the solid phase by the capture antibody allowing the speci�c antibody-antigen reaction to occur in the absence of competing isotypes; soluble antigen is added in excess (allowing the antigen and antibody to react in antigen excess); and the antibody capture ELISA measures the proportion of speci�c to total antibody of a given isotype (which may be detectable earlier than simply measuring speci�c antibodies as in an ELISA test).

Pro TechEx -Antibody Capture ELISA Teaching Kit


Pro TechEx-Ion Exchange Chromatography (Lysozyme Puri�cation) Teaching Kit 5 Exps 3500.00OP-3508-5xp

COMPONENTS

CM-Cellulose (4°C)Column (RT)Solution I (4°C)Solution II (4°C)1M NaCl (RT)

Micrococcus luteus (4°C)Standard lysozyme(4°C)Phosphate Buffer (RT)Protocol


Pro TechEx-Paper Chromatography Teaching Kit 10 Exps 3100.00OP-3509-10xp

Cat.No ExperimentsProduct Price (`)

Pro TechEx-Thin Layer Chromatography Teaching Kit 10 Exps 3700.00OP-3510-10xp


Pro TechEx -Antibody Capture ELISA Teaching Kit 5 Exps 2600.00OP-3601-5xp

COMPONENTS

Chromatography Paper (RT)Proline (4°C)Histidine (4°C)Iso- Leucine (4°C)

Solvents (BAW) (RT)Ninhydrin Reagent (RT)Rf value standard chart sheet (RT)Protocol

COMPONENTS

TLC Plate (RT)Proline (4°C)Histidine (4°C)Leucine (4°C)

Solvents (BAW) (RT)Ninhydrin Reagent (RT)Rf value standard chart sheet (RT)Protocol

ELISA TECHNIQUES

COMPONENTS

Antigen (-20°C)Blocking buffer (4°C)Test Antibody (-20°C)

Antibody (Stock) (-20°C)Secondary Antibody( HRP- Conjugate) (-20°C)Coating Buffer (4°C)

10X PBS (RT)96 well plate (RT)Stop solution (RT)

Substrate (4°C)Wash buffer (RT)Protocol

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Antigen capture ELISA method is the most useful Immunosorbent assay for detecting antigen, since it is 2-5 folds more sensitive than those assay in which antigen is directly bound onto the solid phase. To utilize this assay, one antibody (the "capture" antibody) is puri�ed and bound to a solid phase.Antigen is then added and allowed to complex with the bound antibody. Unbound products are then removed with a wash, and a labeled second antibody (the "detection" antibody) is allowed to bind to the antigen, thus completing the "sandwich". The assay is then quantitated by measuring the amount of labeled second antibody bound to the matrix, through the use of a colorimetric substrate.

Pro TechEx -Antigen Capture ELISA Teaching Kit

In this technique, antibody is �rst incubated in solution with a sample containing antigen. The antigen-antibody mixture is then added to an antigen coated microtiter well. The more antigen present in the sample, the less free antibody will be available to bind to the antigen-coated well. Addition of an enzyme-conjugated secondary antibody (Ab2) speci�c for the isotype of the primary antibody can be used to determine the amount of primary antibody bound to the well as in an indirect ELISA. In the competitive assay, however, the higher the concentration of antigen in the original sample, the lower the absorbance.

Pro TechEx - Competitive ELISA Teaching Kit

In Dot ELISA,the antigen is sandwiched directly between two antibodies which react with two different epitopes on the same antigen. Antigen in the test sample �rst reacts with the immobilized antibody and then with the enzyme-linked secondary antibody. The amount of enzyme linked antibody bound is assayed by incubating the strip with an appropriate chromogenic substrate, which is converted to a coloured, insoluble product. The latter precipitates onto the strip in the area of enzyme activity, hence the name Dot-ELISA. The enzyme activity is indicated by intensity of the spot, which is directly proportional to the antigen concentration.

Pro TechEx - Dot ELISA Teaching Kit

The Sandwich ELISA measures the amount of antigen between two layers of antibodies (i.e. capture and detection antibody). The antigen to be measured must contain at least two antigenic sites capable of binding to antibody, since at least two antibodies act in the sandwich.

Either monoclonal or polyclonal antibodies can be used as the capture and detection antibodies in Sandwich ELISA systems. Monoclonal antibodies recognize a single epitope that allows �ne detection and quanti�cation of small differences in antigen. A polyclonal is often used as the capture antibody to pull down as much of the antigen as possible.

Pro TechEx -Sandwich ELISA Teaching Kit


Pro TechEx -Antigen Capture ELISA Teaching Kit 5 Exps 2600.00OP-3602-5xp


Pro TechEx -Competitive ELISA Teaching Kit 5 Exps 2600.00OP-3603-5xp


Pro TechEx -Dot ELISA Teaching Kit 15 Exps 2600.00OP-3604-15xp

COMPONENTS

Antibody (-20°C)Antigen (1mg/ml stock) (-20°C)Blocking buffer (4°C)Coating Buffer (4°C)Secondary Antibody( HRP- Conjugate) (-20°C)Test Antigen (-20°C)

10X PBS (RT)96 well plate (RT)Stop solution (RT)Substrate (4°C)Wash buffer (RT)Protocol

COMPONENTS

Antibody (-20°C)Antigen (1mg/ml stock) (-20°C)Blocking buffer (4°C)Coating Buffer (4°C)Secondary Antibody( HRP- Conjugate) (-20°C)Test Antigen (-20°C)

10X PBS (RT)96 well plate (RT)Stop solution (RT)Substrate (4°C)Wash buffer (RT)Protocol

COMPONENTS

Dot ELISA Strips (RT)Protein-1 (4°C)Protein-2 (4°C)Blocker (4°C)10X Assay Buffer (RT)Test Serum Sample-A (4°C)Test Serum Sample-B (4°C)

Test Serum Sample-C (4°C)20X Antibody-HRP Conjugate (4°C)Substrate buffer (4°C)DAB(Chromogen) (4°C)H

2O

2(Substrate) (4°C)

10X Wash Buffer (RT)Protocol

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Pro TechEx -Sandwich ELISA Teaching Kit 5 Exps 2600.00OP-3605-5xp

COMPONENTS

Antigen (-20°C)Antibody (4°C)Coating buffer (4°C)Blocker (4°C)96 well plate (RT)1X PBST (RT)

10X Wash Buffer (RT)Antibody-HRP Conjugate (4°C)Stop solution (4°C)DAB(Chromogen) (4°C)H

2O

2(Substrate) (4°C)

Protocol

HAEMATOLOGY

The important components in blood are red cells, white cells, platelets and plasma.Red cells are relatively large microscopic cells without nuclei and transport oxygen from the lungs to all of the living tissues of the body and carry away carbon dioxide. The red cells are produced continuously in our bone marrow from stem cells at a rate of about 2-3 million cells per second. White cells, or leukocytes occur elsewhere in the body as well, most notably in the spleen, liver, and lymph glands. Platelets or thrombocytes are cell fragments without nuclei that work with blood clotting chemicals at the site of wounds. They do this by adhering to the walls of blood vessels, thereby plugging the rupture in the vascular wall. They also can release coagulating chemicals which cause clots to form in the blood that can plug up narrowed blood vessels.Plasma is the relatively clear, yellow tinted water (92+%), sugar, fat, protein and salt solution which carries the red cells, white cells, and platelets.

Pro TechEx - Analysis of Blood components Teaching Kit

The accurate, reproducible, and timely reporting of nucleated red blood cells (NRBC) is an important function of the clinical hematology laboratory. The number of RBC in a known volume of diluted blood is counted and the number of cells in one cmm of undiluted blood is calculat-ed from this.

Pro TechEx - Enumeration Of RBC with Hemocytometer Teaching Kit

The accurate, reproducible, and timely reporting of nucleated red blood cells (NRBC) is an important function of the clinical hematology laboratory. The number of RBC in a known volume of diluted blood is counted and the number of cells in one cmm of undiluted blood is calculat-ed from this.

Pro TechEx - Enumeration Of RBC without Hemocytometer Teaching Kit


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COMPONENTS

Lancette (RT)Glass Slide( RT)Leishman's stain (RT)Buffer (RT)Pasteur pipette (RT)Protocol


Pro TechEx - Enumeration Of RBC with Hemocytometer Teaching Kit 10 Exps 2300.00OP-3702-10xp

COMPONENTS

RBC Diluting �uid (RT)Hemocytometer (RT)Protocol


Pro TechEx - Enumeration Of RBC without Hemocytometer Teaching Kit 10 Exps 1000.00OP-3703-10xp

COMPONENTS

RBC diluting �uid (RT)Protocol

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A white blood cell count estimate the total number of white cells in a cubic millimeter (mm3) of blood. It is important in the diagnosis of disease especially when accompanied by a differential white cell count.

Pro TechEx - Enumeration Of WBC with Hemocytometer Teaching Kit

A white blood cell count estimate the total number of white cells in a cubic millimeter (mm3) of blood. It is important in the diagnosis of disease especially when accompanied by a differential white cell count.

Pro TechEx - Enumeration Of WBC without Hemocytometer Teaching Kit

Measurement of D-glucose is extremely important in biochemistry and clinical analysis, as well as in food analysis; it is mostly determined along with other carbohydrates.The amount of NADPH formed through the combined action of hexokinase (HK) and glucose-6-P dehydroge-nase (G6PDH), measured at 340 nm, is stoichiometric with the amount of D-glucose in sample volume.

Pro TechEx - GOD/POD Teaching Kit

This is formed by the action of a chemical called cyanide (for example. Potassium cyanide, KCN). The combination is reversible. Hemiglobin-cyanide is the methemoglobin bonded to cyanide ions. To measure accurately the total Hb in the blood, it is essential to prepare a stable deriva-tive that will contain all the a Hb forms (complexes) that are present in the blood. All forms of circulating hemoglobin are readily converted to hemoglobin-cyanide (cyanmethemoglobin), except for sulfhemoglobin which is normally not present in the blood. Therefore, the cyanmethemo-globin method is the most accurate method for the determination of hemoglobin.

Pro TechEx -Haemoglobin (Hb) Estimation By Cyanmethaemoglobin Method Teaching Kit

Employing a mixture of Ficoll one can obtain gradients of speci�ed density and osmolarity suited to the separation of cell types based on buoyant density. The speci�c density of the gradient that is needed for successful cell separation depends on the species. Human peripheral blood can be puri�ed from diluted whole blood using Ficoll Medium.

Pro TechEx -Isolation Of Lymphocytes Teaching Kit


Pro TechEx - Enumeration Of WBC with Hemocytometer Teaching Kit 10 Exps 2300.00OP-3704-10xp


Pro TechEx - Enumeration Of WBC without Hemocytometer Teaching Kit 10 Exps 1000.00OP-3705-10xp

COMPONENTS

WBC diluting �uid(RT)Hemocytometer (RT)Protocol

COMPONENTS

WBC Diluting �uid (RT)Protocol


Pro TechEx - GOD/POD Teaching Kit 20 Exps 1000.00OP-3706-20xp

COMPONENTS

Glucose reagent (4°C)Glucose standard (10mg/dL) (4°C)Microfuge Tubes (RT)Protocol

COMPONENTS

Drabkin's solution (-20°C)Cyanmethaemoglobin (-20°C)Protocol

D-Glucose + H2O+O2 D-Glucose acid+H2O2

GOD

H2O+4-aminoantipyrine+phenol Quinoneiminedye+H2OPOD


Pro TechEx -Haemoglobin (Hb) Estimation By Cyanmethaemoglobin Method Teaching Kit 5 Exps 2100.00OP-3707-5xp

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Pro TechEx - Isolation Of Lymphocytes Teaching Kit 5 Exps 1800.00OP-3708-5xp

COMPONENTS

Lymphocyte separation medium(LSM) (4°C)Falcons Tubes (RT)HBSS (RT)Leishman’s stain (RT)Pasteur pipette (RT)Protocol

In this method monocytes isolated by �coll method,further The Monocyte Attachment Medium(DMEM) allows for the ef�cient adherence selection of monocytes from freshly isolated human mononuclear cells while maintaining optimal cell health

Pro TechEx -Isolation Of Monocytes Teaching Kit

Erythrocyte rosetting or E-rosetting is a phenomenon seen through a microscope where red blood cells (erythrocytes) are arranged around a central cell to form a cluster that looks like a �ower. The red blood cells surrounding the cell form the petal, while the central cell forms the stigma of the �ower shape. This formation occurs due to an immunological reaction between an epitope on the central cells surface and a receptor or antibody on a red blood cell. The presence of E-rosetting can be used as a test for T cells although more modern tests such as Immunohistochemistry are available. May be caused by Plasmodium in malaria.

Pro TechEx -Isolation of Rosette Cells Teaching kit

This pregnancy test kit contains everything you need to simply and accurately �nd out wheather your sample contains hCG antigen. Put 3-4 drops of urine(sample collected from pregnant women) in the sample well of the Slide and wait for �ve (5)minutes. The test result in the form of red to magenta (reddish purple) lines or bars will appear in the Result Area of the slidewhere antibodies are coated.

Pro TechEx -Pregnancy Test (Card Method) Teaching Kit


Pro TechEx -Isolation Of Monocytes Teaching Kit 5 Exps 1800.00OP-3709-5xp


Pro TechEx -Isolation of Rosette Cells Teaching kit 10 Exps 3400.00OP-3710-10xp


Pro TechEx -Pregnancy Test (Card Method) Teaching Kit 5 Exps 600.00OP-3711-5xp

COMPONENTS

Monocyte separation medium(MSM) (4°C)Falcons Tubes (RT)HBSS (RT)Leishman’s stain (RT)Pasteur pipette (RT)Protocol

COMPONENTS

Falcons tube(15ml) (RT)Leishman’s stain (RT)Lymphosep (4°C)Microfuge tubes (RT)Microscopic slide (RT)Pasteur’s pipette (RT)PBS buffer (4°C)Protocol

COMPONENTS

Disposable dropper (RT) Card( 4°C)Protocol

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Slide test for pregnancy utilizes the principle of latex agglutination inhibition. The urine specimen to be tested is �rst mixed with the antibody reagent containing antibodies directed against the beta subunit of hCG. Then hCG coupled latex reagent is added and the mixture is allowed to react. When the urine specimen is from a non-pregnant woman and does not contain hCG, the anti-beta hCG monoclonal antibodies will be free to react with latex coupled hCG causing agglutination. When the urine is from a pregnant woman and contains at least 0.3 IU/ml of hCG, the anti-beta hCG monoclonal antibodies will be neutralized and will not react with the latex coupled hCG antigen. Hence no agglutination will be observed.

Pro TechEx -Pregnancy Test (Slide Method) Teaching Kit

The aminotransferases catalyze the chemical reactions involving the amino acids, where an amino group is transferred from the donor amino acid to the recipient molecule. Aminotransferases are also referred to as transaminases. Another name for AST is serum glutamic oxaloacetic transaminase (SGOT)

Pro TechEx -SGOT Teaching Kit

The aminotransferases catalyze the chemical reactions involving the amino acids, where an amino group is transferred from the donor amino acid to the recipient molecule. Aminotransferases are also referred to as transaminases. Another name for ALT is serum glutamic pyruvic transaminase (SGPT).

Pro TechEx -SGPT Teaching Kit


Pro TechEx -Pregnancy Test (Slide Method) Teaching Kit 10 Exps 950.00OP-3712-10xp

COMPONENTS

β-hCG antibody/antisera (4°C)hCG agglutinating antigen (4°C)Glass slide (RT)Disposable mixing sticks (RT)Protocol


Pro TechEx -SGOT Teaching Kit 20 Exps 1300.00OP-3713-20xp


Pro TechEx -SGPT Teaching Kit 20 Exps 1300.00OP-3714-20xp

COMPONENTS

DNPH Reagent (4°C)NaOH 4N (4°C)Pyruvate standard (4°C)Substrate reagent (4°C)Protocol

COMPONENTS

DNPH Reagent (4°C)NaOH 4N (4°C)Pyruvate standard (4°C)Substrate reagent (4°C)Protocol

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PRECIPITATION TECHNIQUES

Immunoelectrophoresis is the electrophoresis of a determined antigen mixture in an agarose gel that allows the separation of different antigens along the gel slide, and then the lateral diffusion of an antibody in the gel. Counter Current Immuno electrophoresis is a modi�cation of immune electrophoresis in which antigen and antibody move in opposite directions and form precipitates in the area between the cells where they meet in concentrations of optimal proportions.

Pro TechEx - Counter Current Immunoelectrophoresis Teaching Kit

In Immuno electrophoresis, the antigen mixture is �rst electrophoresis to separate its constituents by charge. The antiserum containing the antibodies added into the troughs diffuses with a plane front to react with the antigens. Due to diffusion, density gradient of the antigen and antibody are obtained and at a speci�c antigen/antibody ratio (equivalence point) huge macromolecules are formed. They form a visible white complex which precipitates as arcs in the gel. The arc is closer to the trough at the point where the antigen is in highest concentration. The method is very speci�c and highly sensitive because distinct zones are formed. In this method it is important that the ratio between the quanti-ties of antigen and antibody should be proper (antibody titer).

Pro TechEx - Immunoelectrophoresis Teaching Kit

Immunoprecipitation is a technique that uses antibodies speci�c to a protein to remove those proteins from solution. The antibody-protein complexes are precipitated out of solution with the addition of an insoluble form of antibody binding proteins. Examples include Protein A, Protein G, Zysorbin, Immunoprecipitin, or the addition of a second antibody to the solution.

Pro TechEx - Immunoprecipitation Teaching Kit


Pro TechEx - Counter Current Immunoelectrophoresis Teaching Kit 10 Exps

20 Exps

2300.00

3600.00

OP-3801-10xp

OP-3801-20xp Pro TechEx - Counter Current Immunoelectrophoresis Teaching Kit


Pro TechEx - Immunoelectrophoresis Teaching Kit 5 Exps

10 Exps

2650.00

4700.00

OP-3802-5xp

OP-3802-10xp Pro TechEx - Immunoelectrophoresis Teaching Kit


Pro TechEx - Immunoprecipitation Teaching Kit 5 Exps 5500.00OP-3803-5xp

COMPONENTS

Agarose (RT)20X Electro Buffer (RT)Glass slides (RT)Antigen (-20°C)Test antiserum (-20°C)

Positive antiserum (-20°C)Template (RT)Gel punch (RT)Protocol

COMPONENTS

Agarose (RT)20X Electro Buffer (RT)Glass slides (RT)Trough cutter (RT)Antigen (-20°C)

Antiserum A (-20°C)Antiserum B (-20°C)Template (RT)Gel punch (RT)Protocol

COMPONENTS

Antibody (-20°C)APS (4°C)Cell lysate (-20°C)Protein A-Slurry (4°C)Separating gel mix (4°C)Stacking buffer (4°C)Washing buffer (RT)

TEMED (4°C)10%SDS (4°C)Destainer (RT)Running buffer (RT)Sample solubilizing buffer (RT)Stainer (RT)Protocol

Ag Ab- +

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To assess the degree of similarity of the antigens. In this test, different antigenic preparations, each containing single antigenic species are allowed to diffuse from separate wells against the antiserum. Depending on the similarity between the antigens, different geometrical pattern is produced between the antigen and antiserum wells.The pattern of lines that form can be interpreted to determine whether the antigens are Pattern of Identity, Pattern of partial Identity and Pattern of Non-Identity.

Pro TechEx - Ouchterlony Double Immuno Diffusion (Pattern) Teaching Kit

Ouchterlony double diffusion (ODD) or immunodiffusion technique is one of the simplest techniques extensively used to check antisera for the presence of antibodies for a particular Ag and to determine its titer value. In ODD assays, solutions of Ag and Ab are placed in adjacent wells cut in agarose gel and are allowed to diffuse radialy. The Ag and Ab concentration are relatively higher near their respective wells. As they diffuse farther from the wells, their concentration decreases. An antigen will react with its speci�c antibody to form an Ag-Ab complex. At one point their concentrations become equivalent and the Ag-Ab complex precipitates to form a precipitin line.

Pro TechEx - Ouchterlony Double Immuno Diffusion (Titration) Teaching Kit

Quantitative precipitin Assay is based on the principle of precipitation reaction between antigen-antibody interactions. Increasing amounts of antigen are added to a constant amount of antibody and the weight of precipitate formed in each tube is determined. Originally precipitin was thought to result simply from the build up of a large three dimensional lattice formed by combining site cross linking of epitopes on different antigen molecule. It is now also known to depend upon the presence of an intact Fc region in the antibody. Precipitation is far less ef�cient with (ab) fragments of antibody, even though they are divalent.

Pro TechEx - Quantitative Preciptin Assay Teaching Kit


Pro TechEx - Ouchterlony Double Immuno Diffusion (Pattern) Teaching Kit 10 Exps

20 Exps

2300.00

4000.00

OP-3804-10xp

OP-3804-20xp Pro TechEx - Ouchterlony Double Immuno Diffusion (Pattern) Teaching Kit

COMPONENTS

Anti-serum-A (-20°C)Anti-serum-B (-20°C)Anti-serum-C (-20°C)Antigen 1 (-20°C)Antigen 2 (-20°C)Antigen 3 (-20°C)Antigen 4 (-20°C)Antigen 5 (-20°C)

Antigen 6 (-20°C)10X Assay Buffer (RT)Agarose (RT)Gel punch (RT)Glass slides (RT)Template (RT)Protocol


Pro TechEx - Ouchterlony Double Immuno Diffusion (Titration) Teaching Kit 10 Exps

20 Exps

1700.00

3000.00

OP-3805-10xp

OP-3805-20xp Pro TechEx - Ouchterlony Double Immuno Diffusion (Titration) Teaching Kit

COMPONENTS

10X Assay buffer (RT)Agarose (RT)Glass slides (RT)Template (RT)

Antigen (-20°C)Antiserum (-20°C)Gel punch (RT)Protocol


Pro TechEx - Quantitative Preciptin Assay Teaching Kit 10 Exps 2300.00OP-3806-10xp

COMPONENTS

0.1M NaOH (RT)Antibody (-20°C)Antigen (-20°C)Microfuge tubes (RT)PBS (RT)Protocol

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In this procedure, appropriately diluted samples are applied to small circular wells cut into an agarose gel which has a speci�c antibody already incorporated in its matrix. When migrating in an electric �eld, the protein of interest will eventually reach a critical point of antigen-anti-body concentrations resulting in the local formation large precipitating complexes. The agarose gel can then be stained and the rocket-shaped precipitate becomes apparent . The position of this peak is directly related to the concentration of the protein of interest.

The antiserum to the antigen to be quanti�ed is incorporated in agarose and gelled on the glass slide. The antigen, in increasing concentra-tions, is placed in wells punched in the set gel. The antigen is then electrophoresed into the antibody containing agarose. The pattern of immu-noprecipitation resembles a rocket and hence the name.

Pro TechEx - Rocket Immunoelectrophoresis Teaching Kit

Single radial immunodiffusion (RID) is used extensively for the quantitative estimation of antigens. The antigen-antibody precipitation is made more sensitive by the incorporation of antiserum in the agarose. Antigen (Ag) is then allowed to diffuse from wells cut in the gel in which the antiserum is uniformly distributed. Initially, as the antigen diffuses out of the well, its concentration is relatively high and soluble antigen-anti-body adducts are formed. However, as Ag diffuses farther from the well, the Ag-Ab complex reacts with more amount of antibody resulting in a lattice that precipitates to form a precipitin ring.

Pro TechEx - Single Radial Immunodiffusion Teaching Kit


Pro TechEx - Rocket Immunoelectrophoresis Teaching Kit 5 Exps 3800.00OP-3807-5xp

COMPONENTS

20X Electro buffer (RT)Agarose (RT)Antigen (-20°C)Antiserum (-20°C)Glass slide (RT)Test antigen (-20°C)

Destainer (RT)Gel punch (RT)Stainer (RT)Template (RT)Protocol


Pro TechEx - Single Radial Immunodiffusion Teaching Kit 10 Exps

20 Exps

2500.00

4700.00

OP-3808-10xp

OP-3808-20xp Pro TechEx - Single Radial Immunodiffusion Teaching Kit

COMPONENTS

10X Normal saline (RT)Agarose (RT)Antibody (10mg /ml) (-20°C)Antigen (10mg /ml) (-20°C)Test Antigen (-20°C)

Gel punch (RT)Glass slides (RT)Template (RT)Protocol

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A sample of organisms from soil or some other source is diluted in water, then spread onto Petri dishes containing agar gel rich in the nutrients the bacteria will need to grow. Scientists select plates that have a large number of colonies, then look for microorganisms that have inhibited the growth of other microorganisms in their vicinity. These microbes are possibly secreting some kind of compound that is killing or inhibiting their neighbors. The isolation of antibiotic and identi�cation of microbes by this method is known as crowded plate technique.

Pro TechEx -Isolation And Identi�cation Of Antibiotic Producing Microorganism Teaching Kit

After the incubation, the presence or absence of the starch in the inoculated medium determines the ability of microorganisms to excrete hydrolytic extra cellular enzymes capable of degrading the starch. The detection based on the hydrolytic activity of the organism, starch in the presence of iodine will impart a blue-black colour to the medium, indicating the absence of starch split by the enzymes and represent a negative reaction. If the starch has been hydrolyzed a clear zone of hydrolysis will surround the growth of the organism, representing a positive reaction.

Pro TechEx -Isolation And Identi�cation Of Enzyme Producing Microorganism Teaching Kit

Bacteria of genus Rhizobium are fast growing Gram-negative rods that are normally involved in nitrogen �xation for the atmosphere. Rhizo-bia are commonly found in the root nodules of legumes;Yeast mannitol agar (YMA) has been recommended for the isolation of Rhizobium, so we used this media for our protocol. YMA contains yeast extract, which provides the bacteria with a nitrogen source, a necessary element for natural Rhizobium function. YMA also contains mannitol, a major one of the many carbohydrates that Rhizobium can utilize for metabolism.On the majority of the plates, white, mucilaginous, raised, semi-translucent colonies con�rms the result for Rhizobium. This �ts the Bergey's manual description of genus Rhizobium as forming "copious extracellular polysaccharide slime."

Pro TechEx-Isolation And Identi�cation Of Rhizobium From Root Nodules Teaching Kit

Isolation of bacteria was performed by making serial dilution of the taken samples and the dilution used for studies were 10-2 and 10-4 . The dilution was spread in Nutrient agar medium plates and media was weighed out and prepared according to the manufacture’s speci�cation, with respect to the given instructions and directions. The plates were incubated at 37°C for 48 hrs. The Pure cultures were obtained based on the color of colony and the pure cultures of the bacterial isolates were subjected to various morphological and biochemical characterization tests to determine the identity of the bacteria and identi�cation done by gram staining method.

Pro TechEx-Isolation And Identi�cation Of Soil Bacteria Teaching Kit

COMPONENTS

Culture (4°C)Toothpicks (RT)Nutrient agar (RT)Protocol

MICROBIOLOGY


Pro TechEx -Isolation And Identi�cation Of Antibiotic Producing Microorganism Teaching Kit 5 Exps 1950.00OP-3901-5xp

COMPONENTS

Starch agar (RT)Gram's Iodine (RT)Protocol

COMPONENTS

YEMA Medium (3.2gm/100ml) (RT)70%Ethanol (RT)0.1% mercuric chloride (RT)Carbol fuchsin (RT)Protocol


Pro TechEx -Isolation And Identi�cation Of Enzyme Producing Microorganism Teaching Kit 5 Exps 1950.00OP-3902-5xp


Pro TechEx- Isolation And Identi�cation Of Rhizobium From Root Nodules Teaching Kit 5 Exps 1950.00OP-3903-5xp

ISOLATION OF MICROBES

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Yeasts are unicellular eukaryotes ranging from 8-15µm in width. They can be isolated from many sources such as curd and fruit juices etc., using suitable media like sabouraud dextrose agar, yeast malt agar etc. One of the industrially important strains of the yeast is Saccharomyces cerevisiae which is used in the production of alcohol, bakery items, food stuffs, animal feed and biochemicals.

Pro TechEx-Isolation And Identi�cation Of Yeast From Curd Or Fruit Juice Teaching Kit

Great variability and type organism present in soil, it is impossible to isolate all microorganisms by single method. Therefore it is necessary to use different media for growth of organisms. To support the growth of fungi different media (for instance: Sabouraud Dextrose Agar- SDA) are employed to isolate different kind of moulds from the soil samples. The fungal colonies are obtained from the soil sample after 3-4 days of incubation. Genera of the isolated colonies can be identi�ed by lactophenol cotton blue staining.

Pro TechEx-Isolation Of Molds From Soil Teaching Kit


Pro TechEx-Isolation And Identi�cation Of Soil Bacteria Teaching Kit 5 Exps 2750.00OP-3904-5xp


Pro TechEx-Isolation Of Molds From Soil Teaching Kit 5 Exps 2750.00OP-3906-5xp

COMPONENTS

YPD (RT)Agar (RT)Streptomycin (4°C)Protocol

COMPONENTS

Sabouraud Dextrose agar (RT)Normal Saline (RT)lactophenol cotton blue (RT)Protocol

Antibiotic sensitivity is the susceptibility of bacteria to antibiotics. Antibiotic susceptibility testing (AST) is usually carried out to determine which antibiotic will be most successful in treating a bacterial infection in vivo. Testing for antibiotic sensitivity is often done by the Kirby-Bauer method. Small wafers containing antibiotics are placed onto a plate upon which bacteria are growing. If the bacteria are sensitive to the antibiot-ic, a clear ring, or zone of inhibition, is seen around the wafer indicating poor growth.

Pro TechEx-Antibiotic Sensitivity Test Teaching Kit


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COMPONENTS

LB broth (RT)Agar (RT)Antibiotic 1 (4°C)Antibiotic 2 (4°C)Protocol


Pro TechEx-Isolation And Identi�cation Of Yeast From Curd Or Fruit Juice Teaching Kit 5 Exps 2750.00OP-3905-5xp

COMPONENTS

Soil Extract Agar (RT)Nutrient broth (RT)Agar ) (RT)Crystal violet (RT)Grams iodine (RT)

Ethyl alcohol (RT)Safranin (RT)Glass slide (RT)Protocol

GROWTH KINETICS TECHNIQUES

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Bacterial growth is the asexual reproduction, or cell division, of a bacterium into two daughter cells, in a process called binary �ssion. Provid-ing no mutational event occurs the resulting daughter cells are genetically identical to the original cell. Hence, "local doubling" of the bacterial population occurs. Both daughter cells from the division do not necessarily survive. However, if the number surviving exceeds unity on average, the bacterial population undergoes exponential growth. The measurement of an exponential bacterial growth curve in batch culture was traditionally a part of the training of all microbiologists; the basic means requires bacterial enumeration (cell counting) by direct and individual can estimate by calorimetric method.

Pro TechEx-Bacterial Growth Curve Teaching Kit

LB broth were buffered with acetic acid at pH 4 and 5, with MES at pH 6 and with HEPES at pH 7 and 8. Each sample of medium was transferred to a 250 ml �ask and 0.5 ml of a saturated overnight culture of E. coli was added to each �ask. A sample was taken from each �ask at times 0, 0.5, 1, 2, 3, and 4 hours. The amount of bacteria in each sample was measured using a spectrophotometer at 595nm. The OD(595nm) and time data were plotted to show growth curves at each pH tested.

Pro TechEx-Effect Of Different pH On Bacterial Growth Teaching Kit

The effect to which salt concentration causes changes in bacterial growth depends on the osmotic balance required for such growth. Some bacteria require an astonishingly high level of salt to begin growth, whereas other bacteria would be immediately killed in high levels of salt.

Pro TechEx-Effect Of Different Salt Concentration On Bacterial Growth Teaching Kit

Bacteria and fungi can grow across a large spectrum of environmental conditions. Even though the bacterium may grow well in the human body at 37°C at pH 7 conditions, it may also be able to withstand out-of-the-ordinary pH, temperature, and osmotic pressure. Basically, there are 3 large groups of microorganisms with respect to their temperature preference -mesophiles, thermophiles, and psychrophiles. Animal pathogens are mesophilic, growing well in the range of 20-45°C. Psychrophiles have an optimal range below mesophiles (even below freezing), while thermophiles grow at temperatures over 45°C.

Pro TechEx- Effect Of Different Temperature On Bacterial Growth Teaching Kit


Pro TechEx-Bacterial Growth Curve Teaching Kit 5 Exps

10 Exps

2700.00

4000.00

OP-4002-5xp

OP-4002-10xp Pro TechEx-Bacterial Growth Curve Teaching Kit

COMPONENTS

Agar (RT)LB broth (RT)Bacterial culture (4°C)Protocol

COMPONENTS

Bacterial culture (4°C)Alkali Buffer (RT)LB Broth (RT)Agar (RT)Protocol


Pro TechEx-Effect Of different pH On Bacterial Growth Teaching Kit 5 Exps 2950.00OP-4003-5xp


Pro TechEx- Effect Of Different Temperature On Bacterial Growth Teaching Kit 5 Exps 2950.00OP-4005-5xp

COMPONENTS

Bacterial Culture (4°C)LB Broth (RT)Agar(RT)NaCl(RT)Protocol


Pro TechEx-Effect Of Different Salt Concentration On Bacterial Growth Teaching Kit 5 Exps 2950.00OP-4004-5xp

COMPONENTS

Bacterial Culture (4°C)LB Broth (RT)

Agar(RT)Protocol

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The acid-fast stain is a laboratory test that determines if a sample of tissue, blood, or other body substance is infected with the bacteria that causes tuberculosis. The lipoid capsule of the acid – fast organism takes up carbolfuchsin and resists decolorization with a dilute acid rinse. The lipoid capsule of the mycobacteria is of such high molecular weight that is waxy at room temperatureand successful penetration by the aqueous based staining solutions (such as gram’s) is prevented .

Pro TechEx-Acid Fast Staining Teaching Kit

Many bacteria have a slimy layer surrounding them,which is usually referred to as a capsule. The capsule's composition, as well as its thickness, varies with individual bacterial species. Polysaccharides, polypeptides, and glycoproteins have all been found in capsules. Often, a pathogenic bacterium with a thick capsule will be more virulent than a strain with little or no capsule since the capsule protects the bacterium against the phagocytic activity of the host's phagocytic cells. The capsule stain is of some importance in clinical microbiology (e.g., in the diagnosis of bacterial pneumonia and the fungus Cryptococcus neoformans)

Anthony's procedure employs two reagents. The primary stain is crystal violet, which gives the bacterial cell and its capsular material a dark purple color.Unlike the cell, the capsule is nonionic and the primary stain cannot adhere. Copper sulfate is the decolorizing agent. It removes excess primary stain as well as color from the capsule. At the same time, the copper sulfate acts as a counterstain by being absorbed into the capsule and turning it a light blue. In this procedure, smears should not be heat-�xed since shrinkage is likely to occur and create a clear zone around the bacterium, which can be mistaken for a capsule.

Pro TechEx- Capsular Staining Teaching Kit

Endospore staining is a technique used in bacteriology to identify the presence of endospores in a bacterial sample, which can be useful for classifying bacteria. Within bacteria, endospores are quite protective structures used to survive extreme conditions, but this protective nature makes them dif�cult to stain using normal techniques. Special techniques for endospore staining include the Schaeffer–Fulton stain and the Moeller stain. A good stain to use for spore staining is malachite green. It takes a long time for the spores to stain due to their density, so time acts as the mordant when doing this differential stain; the slide with the bacterium should be soaked in malachite green for at least 30 minutes. Water acts as the decolorizer. A counterstain to differentiate the vegetative cells is 0.5% safranin. Types of endospores that could be identi�ed are free endospores, central endospores, central and swollen endospores, and subterminal endospores.

Pro TechEx-Endospore Staining Teaching Kit


Pro TechEx-Acid Fast Staining Teaching Kit 25 Exps 3600.00OP-4101-25xp


Pro TechEx-Capsular Staining Teaching Kit 25 Exps 3200.00OP-4102-25xp

COMPONENTS

Sample culture (4°C)Primary stain (RT)Decolorizer (RT)Counter stain (RT)Glass slides (RT)Absorbent paper (RT)Protocol

COMPONENTS

Culture (4°C)Crystal violet stain (RT)Copper sulphate solution (20%).(RT)Glass Slide (RT)Filter paper (RT)Protocol


Pro TechEx-Endospore Staining Teaching Kit 25 Exps 3500.00OP-4103-25xp

COMPONENTS

Primary stain (RT)Counter stain (RT)Glass slide (RT)Bacillus culture (4°C)Filter paper (RT)Protocol

STAINING TECHNIQUES

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Bacterial �agella are �ne, thread like organelles for locomotion. They are slender (about 10 to 30nm in diameter) and can only be seen directly using the electron microscope. In order to observe them with the light microscope, the thickness of the �agella are increased by coating them with mordants like tannic acid and potassium alum, and staining them with basic fuchsin (Gray method), pararosaniline (Leifson method), silver nitrate (West method) or crystal violet (Difco's method). Although �agella staining procedures are dif�cult to carry out, they often provide information about the presence and location of �agella, which is of great value in bacterial identi�cation.

Pro TechEx-Flagella Staining Teaching Kit

Gram staining, also called Gram's Method, is a method of differentiating bacterial species into two large groups (gram-positive and gram-negative). The name comes from its inventor, Hans Christian Gram.

Gram staining consists of four components: • Primary stain (Crystal violet, methyl violet or Gentian violet) • Mordant (Gram's Iodine) • Decolourizer (ethyl alcohol, acetone or 1:1 ethanol-acetone mixture) • Counterstain (Dilute carbol fuchsin, safranin or neutral red)

Pro TechEx-Gram Staining Teaching Kit

Fungi are very dif�cult to visualize without the aid of special Staining procedure. Lactic acid act as a preservative for fungi. The phenol portion kills the fungi. The cotton blue stains the fungal elements that results Fungal elements are stained a deep blue; background is pale blue. Do not overstain.

Pro TechEx-Lactophenol Cotton Blue Staining Teaching Kit

Albert's staining technique is a type of special staining technique since it is used to demonstrate a special structure in bacteria. It is chie�y used to demonstrate metachromatic granules found in Corynebacterium diphtheriae. The storage granules in this bacterium is called metachromatic granules because it exhibits the property of metachromasia, wherein the granules appear in a colour other than the colour used for staining. When stained with polychrome methylene blue, the granules appear violet while the rest of the bacillus appears blue.

Green coloured, rod shaped bacteria that are arranged at angles to each other resembling English letter 'L', 'V' or Chinese letter pattern along with bluish black metachromatic granules at the poles seen.

Pro TechEx-Metachromatic Granules Staining Teaching Kit


Pro TechEx-Flagella Staining Teaching Kit 25 Exps 3300.00OP-4104-25xp


Pro TechEx-Gram Staining Teaching Kit 25 Exps 3330.00OP-4105-25xp

COMPONENTS

Solution A (RT)Solution B (RT)Filter paper (RT)Glass slide (RT)Protocol

COMPONENTS

Primary Stain (RT)Mordant (RT) Decolourizer (RT)Secondary Stain (RT)Glass slides (RT)Filter paper (RT)Protocol


Pro TechEx-Lactophenol Cotton Blue Staining Teaching Kit 25 Exps 2300.00OP-4106-25xp

COMPONENTS

Fungal culture (4°C)lactophenol stain (RT)Glass slide (RT)Protocol

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The use of single stain to colour the bacteria is commonly called as monochrome staining. (Mono-single, Chrome-color)The method of staining utilizes the knowledge that bacteria possess slight negative charge. The negative charged group of bacterial cell surface produces attraction between basic stains. The stains used in this method include methylene blue, crystal violet,etc .

Pro TechEx-Monochrome Staining Teaching Kit

Negative staining is an established method, often used in diagnostic microscopy, for contrasting a thin specimen with an optically opaque �uid. In this technique, the background is stained, leaving the actual specimen untouched, and thus visible. This contrast with 'positive staining', in which the actual specimen is stained. Negative stains are even simpler than simple stains because you do not have to make a smear. A drop of cells is spread on a slide and viewed without �xation. The stain is a suspension of carbon, found in India ink or nigrosin. The carbon particles are negatively-charged, as is the cell membrane. The background looks black or sepia colored and the cells remain clear, since they repel the dye. Some positively charged inclusion bodies such as sulfur may stain. This stain gives accurate information on cell morphology and capsule presence because the cells are not �xed.

Pro TechEx-Negative Staining Teaching Kit

Milk is fermented by inoculating appropriate bacteria. The bacteria that developed can be stained by simple staining method.Basic stains with a positively charged chromogen are preferred because bacterial nucleic acids and certain cell wall components carry a negative charge that strongly attracts and binds to the cationic chromogen. The purpose of simple staining is to elucidate the morphology and arrangements of bacterial cells in milk. The commonly used basic stains are Methylene Blue, Crystal Violet, and Carbol Fuchsin.

Pro TechEx-Simple Staining Method For Bacteria In Milk Teaching Kit


Pro TechEx-Monochrome Staining Teaching Kit 25 Exps 2625.00OP-4108-25xp


Pro TechEx-Metachromatic Granules Staining Teaching Kit 25 Exps 2625.00OP-4107-25xp

COMPONENTS

Bacterial culture (4°C)Glass slide (RT)Albert stain (RT)Absorbent paper (RT)Protocol

COMPONENTS

Bacterial strain (4°C)Methylene blue stain (RT)Glass slide (RT)Absorbent paper (RT)Protocol


Pro TechEx-Negative Staining Teaching Kit 25 Exps 2450.00OP-4109-25xp

COMPONENTS

Bacterial strain (4°C)Nigrosin (RT)Glass Slide (RT)Protocol


Pro TechEx-Simple Staining Method For Bacteria In Milk Teaching Kit 25 Exps 2400.00OP-4110-25xp

COMPONENTS

Methylene Blue (RT)Crystal violet (RT)Carbol fuchsin (RT)Protocol

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The purpose of simple staining is to elucidate the morphology and arrangements of bacterial cells in milk. The commonly used basic stains are Methylene Blue, Crystal Violet, and Carbol Fuchsin.

Pro TechEx-Simple Staining Teaching Kit


Pro TechEx-Simple Staining Teaching Kit 25 Exps 2600.00OP-4111-25xp

COMPONENTS

Methylene blue (RT)Glass slides (RT)Bacterial Culture (4°C)Absorbent papers (RT)Protocol

Plant tissue culture is a collection of techniques used to maintain or grow plant cells, tissues or organs under sterile conditions on a nutrient culture medium of known composition. Plant tissue culture is widely used to produce clones of a plant in a method known as micropropagation.

Pro TechEx-Plant Tissue Culture Teaching Kit


Pro TechEx-Plant Tissue Culture Teaching Kit 5 Exps 5250.00OP-4201-5xp

COMPONENTS

Callus induction media (4°C)Hormones 1 (4°C)Shoot induction media (4°C)Hormones 2 (4°C)Root induction media (4°C)Hormones 3 (4°C)10% Bleach sterilizing solution (4°C)70% alcohol (4°C)

TISSUE CULTURE TECHNIQUES

Hela cells can be grown to the appropriate density usually 70% in a humidi�ed chamber at 37°C. HeLa cells in many labs are cultured in 75 cm2 �asks (or medium �asks). HeLa cells are grown in Dulbecco's Modi�ed Eagle Medium with10% fetal bovine serum (FCS) and 1% MEM non-essential amino acids with penicillin-streptomycin at 1% is added to the culture media.

Pro TechEx- Culture Of HeLa S3 Cells In Suspension ( without CO2 System) Teaching Kit

Chicken eggs were used for preparation of cell culture at 11,12, or 13 days of incubation. The disinfected egg’s air-sac region were cut and the embryo was removed from the shell. The embryo’s �broblast region was processed with trypsinization.

Pro TechEx-Preparation Of Chicken Embryo Fibroblast (In CO2 Incubator) Teaching Kit


Pro TechEx- Culture Of HeLa S3 Cells In Suspension ( without CO2 System) Teaching Kit 5 Exps 8200.00OP-4301-5xp

COMPONENTS

MTT (4°C)Cell line (4°C)DMEM (4°C)Tryphan blue (RT)Protocol


Pro TechEx-Preparation Of Chicken Embryo Fibroblast (In CO2 Incubator) Teaching Kit 5 Exps 7800.00OP-4302-5xp

ANIMAL TISSUE CULTURE

COMPONENTS

MTT (4°C)Cell line (4°C)DMEM (4°C)Trypsin (4°C)

FBSA(4°C)Tryphan blue(RT)Protocol

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The MTT assay is a colorimetric assay for assessing cell viability. NAD(P)H-dependent cellular oxidoreductase enzymes may, under de�ned conditions, re�ect the number of viable cells present. These enzymes are capable of reducing the tetrazolium dye MTT 3-(4,5-dimethylthi-azol-2-yl)-2,5-diphenyltetrazolium bromide to its insoluble formazan, which has a purple colort. Tetrazolium dye assays can also be used to measure cytotoxicity (loss of viable cells) or cytostatic activity (shift from proliferation to quiescence) of potential medicinal agents and toxic materials. MTT assays are usually done in the dark since the MTT reagent is sensitive to light.

Pro TechEx-MTT Assay Teaching Kit

Determination of apoptosis By Staining Dye is primary method. Two types of dyes are used to study apoptosis are Hematoxylin Staining and Giemsa Staining.

Pro TechEx-Determination Of Apoptosis By Staining Method Teaching Kit


Pro TechEx-MTT Assay Teaching Kit 5 Exps 3600.00OP-4401-5xp

COMPONENTS

MTT (4°C)Cell line (4°C)DMEM (4°C)Tryphan blue(RT)Protocol


Pro TechEx-Determination Of Apoptosis By Staining Method Teaching Kit 5 Exps 8500.00OP-4402-5xp

COMPONENTS

Cell line (4°C)DMEM (4°C)Hematoxylin (4°C)Giemsa stain (RT)Protocol

APOPTOSIS

118

CONT

ENTS

DNA , Plasmid & Vectors Genomic DNA From Bacillus subtilis 119

Genomic DNA From Escherichia coli 119

Genomic DNA from Human Blood 119

Genomic DNA from Plant 119

Genomic DNA from Pseudomonas aeuroginosa 119

Genomic DNA from Yeast 119

Lambda DNA 119

pBR 322 119

pUC 18 DNA 119

PUC 19 DNA 119

DNA Markers 100bp DNA Ladder 120

1Kb DNA Ladder 120

Lambda DNA + ECORI Marker 120

Lambda Hind III + ECORI Marker 120

Lambda Hind III Marker 120

Protein molecular weight Marker Low Range 120

Medium Range 120

Broad Range 120

High range 120

ENZYMES Modifying Enzymes

Alkaline Phosphatase 121

DNase I 121

Horse raddish Peroxidase 121

Lysozyme 121

Proteinase K 121

RNase A 121

RNase Inhibitor 121

T4 DNA Ligase 121

Restriction Enzymes Alu I 122

Bam H I 122

ECOR I 122

ECOR V 122

Hind III 122

Pst I 122

Sal I 122

Sma I 122

Xba I 122

DNA, Markers & Enzyme

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DNADNA, Plasmids & Vectors

Cat.No Product Volume Price (`)

Pro CheMol- Genomic DNA From Bacillus subtilis

950.00

3800.00

7000.00

950.00

3800.00

7000.00

950.00

3800.00

7000.00

950.00

3800.00

7000.00

950.00

3800.00

7000.00

950.00

3800.00

7000.00

1200.00

2000.00

3400.00

1200.00

4800.00

7600.00

1200.00

4800.00

7600.00

1200.00

4800.00

7600.00

OP-4501-10µg

OP-4501- 50µg

OP-4501-100µg

10µg

50µg

100µg

Pro CheMol- Genomic DNA From Escherichia coli

OP-4502-10µg

OP-4502- 50µg

OP-4502-100µg

10µg

50µg

100µg

Pro CheMol- Genomic DNA from Human Blood

OP-4503-10µg

OP-4503- 50µg

OP-4503-100µg

10µg

50µg

100µg

Pro CheMol- Genomic DNA from Plant

OP-4504-10µg

OP-4504- 50µg

OP-4504-100µg

10µg

50µg

100µg

Pro CheMol- Genomic DNA from Pseudomonas aeuroginosa

OP-4505-10µg

OP-4505- 50µg

OP-4505-100µg

10µg

50µg

100µg

Pro CheMol- Genomic DNA from Yeast

OP-4506-10µg

OP-4506- 50µg

OP-4506-100µg

10µg

50µg

100µg

Pro CheMol- Lambda DNA

OP-4507-50µg

OP-4507-100µg

OP-4507-200µg

50µg

100µg

200µg

Pro CheMol- pBR 322

OP-4508-10µg

OP-4508- 50µg

OP-4508-100µg

10µg

50µg

100µg

Pro CheMol- pUC 18 DNA

OP-4509-10µg

OP-4509- 50µg

OP-4509-100µg

10µg

50µg

100µg

Pro CheMol- pUC 19 DNA

OP-4510-10µg

OP-4510- 50µg

OP-4510-100µg

10µg

50µg

100µg

120

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Pro CheMol- 100bp DNA Ladder

P.O.R*

P.O.R*

P.O.R*

OP-4601-20Rx

OP-4601-100Rx

OP-4601-250Rx

10µg(20Rx)

50µg(100Rx)

125µg(250Rx)

Pro CheMol- 1Kb DNA Ladder

P.O.R*

P.O.R*

P.O.R*

OP-4602-20Rx

OP-4602-100Rx

OP-4602-250Rx

10µg(20Rx)

50µg(100Rx)

125µg(250Rx)

Pro CheMol- Lambda DNA + ECORI Marker

P.O.R*

P.O.R*

P.O.R*

OP-4603-20Rx

OP-4603-100Rx

OP-4603-250Rx

10µg(20Rx)

50µg(100Rx)

125µg(250Rx)

Pro CheMol- Lambda Hind III + ECORI Marker

P.O.R*

P.O.R*

P.O.R*

OP-4604-20Rx

OP-4604-100Rx

OP-4604-250Rx

10µg(20Rx)

50µg(100Rx)

125µg(250Rx)

Pro CheMol- Lambda Hind III Marker

P.O.R*

P.O.R*

P.O.R*

OP-4605-20Rx

OP-4605-100Rx

OP-4605-250Rx

10µg(20Rx)

50µg(100Rx)

125µg(250Rx)

DNA Markers


Pro CheMol - Protein Marker - Low Range

P.O.R*

P.O.R*

P.O.R*

OP-4701-20Rx

OP-4701-100Rx

OP-4701-250Rx

10µg(20Rx)

50µg(100Rx)

125µg(250Rx)

Pro CheMol - Protein Marker - Medium Range

P.O.R*

P.O.R*

P.O.R*

OP-4702-20Rx

OP-4702-100Rx

OP-4702-250Rx

10µg(20Rx)

50µg(100Rx)

125µg(250Rx)

Pro CheMol - Protein Marker - Broad Range

P.O.R*

P.O.R*

P.O.R*

OP-4703-20Rx

OP-4703-100Rx

OP-4703-250Rx

10µg(20Rx)

50µg(100Rx)

125µg(250Rx)

Pro CheMol - Protein Marker -High RangeP.O.R*

P.O.R*

OP-4704-40Rx

OP-4704-100Rx

20µg(40Rx)

50µg(100Rx)

Protein Molecular Weight Marker

*Price on Request

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ENZYMESModifying Enzymes


Alkaline Phosphatase

950.00

1600.00

2500.00

1200.00

2100.00

4050.00

700.00

1300.00

2500.00

520.00

950.00

4650.00

300.00

475.00

1150.00

1200.00

2650.00

5800.00

750.00

1400.00

2800.00

1200.00

2100.00

4000.00

OP-4801-5mg

OP-4801-10mg

OP-4801-25mg

5mg

10mg

25mg

DNase I

OP-4802-250U

OP-4802-500U

OP-4802-1KU

250U

500U

1000U

Horse Radish Peroxidase

OP-4803-500U

OP-4803-1KU

OP-4803-2KU

500U

1000U

2000U

Lysozyme

OP-4804-500mg

OP-4804-1gm

OP-4804-5gm

500mg

1gm

5gm

Proteinase K

OP-4805-5mg

OP-4805-10mg

OP-4805-25mg

5mg

10mg

25mg

RNase A

OP-4806-10mg

OP-4806-25mg

OP-4806-50mg

10mg

25mg

50mg

RNase Inhibitor

OP-4807-500U

OP-4807-1KU

OP-4807-2KU

500U

1000U

2000U

T4 DNA Ligase

OP-4808-500U

OP-4808-1KU

OP-4808-2KU

500U

1000U

2000U

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Restriction Enzymes


Alu I 1100.00

1950.00

3840.00

430.00

760.00

1520.00

240.00

430.00

860.00

1410.00

2510.00

4900.00

220.00

400.00

775.00

440.00

810.00

1610.00

580.00

910.00

1770.00

700.00

1050.00

2100.00

820.00

1050.00

2100.00

OP-4901-250U

OP-4901-500U

OP-4901-1KU

250U

500U

1000U

OP-4902-250U

OP-4902-500U

OP-4902-1KU

250U

500U

1000U

OP-4903-250U

OP-4903-500U

OP-4903-1KU

250U

500U

1000U

OP-4904-250U

OP-4904-500U

OP-4904-1KU

250U

500U

1000U

OP-4905-250U

OP-4905-500U

OP-4905-1KU

250U

500U

1000U

OP-4906-250U

OP-4906-500U

OP-4906-1KU

250U

500U

1000U

OP-4907-250U

OP-4907-500U

OP-4907-1KU

250U

500U

1000U

OP-4908-250U

OP-4908-500U

OP-4908-1KU

250U

500U

1000U

OP-4909-250U

OP-4909-500U

OP-4909-1KU

250U

500U

1000U

5’ ... A G C T ... 3’ 3’ ... T C G A ... 5’

Source : An E. coli strain that carries the cloned AluI gene from Arthrobacter luteus (ATCC 21606).

Bam HI

Source : Bacillus amyloliquefaciens

5’ ... G G A T C C ... 3’3’ ... C C T A G G ... 5’

EcoR I

Source : EcoRI (pronounced, "eco R one") is an Endonuclease enzyme isolated from strains of E. coli

5’ ... G A A T T C ... 3’3’ ... C T T A A G ... 5’

EcoR V

Source : EcoRV (pronounced, "eco R �ve") is an Endonuclease enzyme isolated from strains of E. coli

5’ ... G A T A T C ... 3’3’ ... C T A T A G ... 5’

Hind III

Source : An E. coli strain that carries the HindIII gene from Haemophilus in�uenzae (ATCC 51907).

5’ ... A A G C T T ... 3’3’ ... T T C G A A ... 5’

Pst 1

Source : An E. coli strain that carries the PstI gene from Providencia stuartii 164 (ATCC 49762).

5’ ... C T G C A G ... 3’3’ ... G A C G T C ... 5’

Sal I

Source : An E. coli strain that carries the SalI gene from Streptomyces albus (ATCC 49789).

5’ ... G T C G A C ... 3’3’ ... C A G C T G ... 5’

Sma I

Source : An E. coli strain that carries the SmaI gene from Serratia marcescens (ATCC 49779).

5’ ... C C C G G G ... 3’3’ ... G G G C C C ... 5’

Xba I

Source : An E. coli strain that carries the XbaI gene from Xanthomonas badrii

5’ ... T C T A G A ...3’3’ ... A G A T C T ...5’

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CONT

ENTS

LABORATORY BUFFERS Pro CheMol - ACK lysis Buffer

Pro CheMol - Assay buffer (10X)

Pro CheMol - FACs buffer

Pro CheMol - GTE Buffer (50mM)

Pro CheMol - HBSS buffer

Pro CheMol - Phosphate Buffer Saline (PBS)10X

Pro CheMol - Phosphate buffer saline Tween-20 (10X) (PBST)

Pro CheMol - RBC lysis Buffer

Pro CheMol - RIPA buffer

Pro CheMol - SM Buffer

Pro CheMol - Sodium Citrate Buffer

Pro CheMol - SSC buffer

Pro CheMol - STET Lysis Buffer

Pro CheMol - TE Buffer (10X)

Pro CheMol - Tris Buffer pH 6.8

Pro CheMol - Tris Buffer pH 8.8

Pro CheMol - Tris buffer saline (TBS) (10X)

Pro CheMol - Tris buffer saline Tween-20 (TBST) (10X)

Pro CheMol - WBC lysis Buffer

Pro CheMol - Whole blood DNA lysis buffer

ELECTROPHORESIS BUFFERSPro CheMol - Blotting Buffer

Pro CheMol - Immuno Buffer (20X)

Pro CheMol - MOPS Buffer (10X)

Pro CheMol - SSPE Buffer (20X)

Pro CheMol - TAE(10X )

Pro CheMol - TAE(50X )

Pro CheMol - TEB(10X)

Pro CheMol - Tris - Glycine Buffer for NATIVE -PAGE (10X)

Pro CheMol - Tris - Glycine -SDS Buffer for SDS-PAGE (10X)

Pro CheMol - Tris-tricine Buffer (10X)

124

124

124

124

124

125

125

125

125

125

126

126

126

126

127

127

127

127

127

128

128

128

128

128

129

129

129

129

129

129

BUFFERS

124

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BUFFERSLABORATORY BUFFERS

The ACK lysis buffer is used to lyse the Red Blood cells.

Pro CheMol -ACK Lysis Buffer


Pro CheMol- ACK lysis Buffer



100ml

250ml

500ml

600.00

1400.00

3000.00

OP-5001-100ml

OP-5001-250ml

OP-5001-500ml

Storage : Room Temperature

Assay Buffer is used in immunotechniques by converting 10X into 1X.

Pro CheMol -Assay Buffer (10X)


Pro CheMol- Assay Buffer (10X)



100ml

250ml

500ml

800.00

1800.00

3200.00

OP-5002-100ml

OP-5002-250ml

OP-5002-500ml


This Buffer reduces the chance of heterophilic antibody interference. Heterophilic antibody interference causes both false positives and false negatives. Presence of the FBS keeps the cells a bit happier during prolonged staining times. Harvest, wash the cells (single cell suspension) and adjust cell number to a concentration of 1-5x10-6 cells/ml in ice cold FACS Buffer.

Pro CheMol -FACs Buffer





100ml

250ml

500ml

3200.00

7150.00

14200.00

OP-5003-100ml

OP-5003-250ml

OP-5003-500ml

Storage : 4°C

GTE is used to resuspend bacterial cell pellets prior to lysing (breaking open) the cells and harvesting the plasmid DNA inside. Lysozyme, which softens the cell membranes, is often added along with the GTE buffer. Achieving a homogenous suspension of whole cells during this step so that the subsequently added lysis solution can get to all of the cells is key to getting good DNA yields. GTE is designed to do this while also providing a stable environment for the DNA.

Pro CheMol -GTE Buffer (50mM)





100ml

250ml

500ml

400.00

790.00

1750.00

OP-5004-100ml

OP-5004-250ml

OP-5004-500ml

Storage : RT

A balanced salt solution (BSS) is a solution made to a physiological pH and salt concentration. Solutions most commonly include sodium, potassium, calcium, magnesium, and chloride. Balanced salt solutions are used for washing tissues and cells and are usually combined with other agents to treat the tissues and cells. They provide the cells with water and inorganic ions, while maintaining a physiological pH and osmot-ic pressure. Sometimes glucose is added as an energy source.

Pro CheMol -HBSS Buffer


125

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Phosphate-buffered saline (abbreviated PBS) is a buffer solution commonly used in biological research. It is a water-based salt solution containing sodium phosphate, sodium chloride and, in some formulations, potassium chloride and potassium phosphate. The osmolarity and ion concentrations of the solutions match those of the human body (isotonic).

Pro CheMol -Phosphate Buffer Saline (PBS) 10X


Pro CheMol -Phosphate Buffer Saline (PBS)10X



100ml

250ml

500ml

720.00

1100.00

2090.00

OP-5006-100ml

OP-5006-250ml

OP-5006-500ml


It is used as washing buffers in ELISA, blotting techniques.

Pro CheMol -Phosphate buffer saline Tween-20 (10X) (PBST)





100ml

250ml

500ml

750.00

1390.00

2350.00

OP-5007-100ml

OP-5007-250ml

OP-5007-500ml


Our RBC Lysis Buffer is designed for the optimal lysis of erythrocytes, while having minimal effect of lymphocytes, this buffer has been used directely. The use of RBC Lysis Buffer allows for the preferential lysis of red blood cells from whole blood and as these are the majority of cells in whole blood permits the concentration of the nucleated white blood cells. This buffer is ideal for the isolation of DNA and RNA from blood.

Pro CheMol -RBC lysis Buffer





100ml

250ml

500ml

750.00

1620.00

3420.00

OP-5008-100ml

OP-5008-250ml

OP-5008-500ml


RIPA lysis buffer (short for radio immunoprecipitation assay buffer) is commonly used to cell lysis prior to immunoprecipitation. It is very useful for the extraction of cellular proteins, but is not appropriate for co-immunoprecipitation studies, as the SDS may disrupt many protein-protein interactions.

Pro CheMol- RIPA buffer


Pro CheMol -RIPA buffer



20ml

50ml

100ml

3090.00

6490.00

12990.00

OP-5009-20ml

OP-5009-50ml

OP-5009-100ml

Storage : 4°C

SM buffer or SM phage diluents which contains gelatin, Chloroform and Mg2+ are used for routine manipulation of phage stocks. The gelatin used in SM buffer helps to stabilize the phage particles while storage. Chloroform maintains the sterility of phage stock by hindering bacterial growth without causing any harm to phage.

Pro CheMol- SM Buffer



Pro CheMol - HBSS Buffer



100ml

250ml

500ml

620.00

1440.00

3050.00

OP-5005-100ml

OP-5005-250ml

OP-5005-500ml

126

BUFF

ERS

PR

OD

UC

T C

ATA

LOG

UE

Buffered Sodium Citrate is used in clinical laboratories and blood banks as an effective anti-coagulant, usually in a ratio of 1:9 sodium citrate/blood. The citrate ion chelates calcium ions in the blood by forming calcium citrate complexes and disrupting the blood clotting mecha-nism. Sodium citrate buffer, or citric acid buffer, is used for RNA isolation because it minimizes base hydrolysis of the RNA strands. This makes it an important step in the mRNA puri�cation, during genomic research or for studying transcription. Citrate-based buffers can also aid the detection of antigens in �xed tissue preparations, because they break the cross-links formed between the antigens and the �xation media.

Pro CheMol- Sodium Citrate Buffer





100ml

250ml

500ml

890.00

1470.00

2750.00

OP-5011-100ml

OP-5011-250ml

OP-5011-500ml


STET Lysis Solution is a commonly used buffer for the lysis of bacterial cells such as E. coli for applications including plasmid DNA mini-prep or RNA isolations. STET Lysis Solution is a ready-to-use solution designed for rapid and ef�cient bacterial lysis.

Pro CheMol- STET Lysis Buffer





100ml

250ml

500ml

790.00

1250.00

2190.00

OP-5013-100ml

OP-5013-250ml

OP-5013-500ml


It used as a Elution buffer for RNA and DNA Extraction.

Pro CheMol- TE Buffer (10X)





100ml

250ml

500ml

1590.00

2790.00

4780.00

OP-5014-100ml

OP-5014-250ml

OP-5014-500ml


• Convenient ready-to-use liquid• Multi-purpose use in hybridizations

Pro CheMol- SSC Buffer (20X)





100ml

250ml

500ml

890.00

1470.00

2750.00

OP-5012-100ml

OP-5012-250ml

OP-5012-500ml






100ml

250ml

500ml

1050.00

2090.00

3950.00

OP-5010-100ml

OP-5010-250ml

OP-5010-500ml

127

BUFF

ERS

PR

OD

UC

T C

ATA

LOG

UE

It is used in stacking gel mix for SDS-PAGE.

Pro CheMol- Tris Buffer pH 6.8





100ml

250ml

500ml

990.00

1800.00

3450.00

OP-5015-100ml

OP-5015-250ml

OP-5015-500ml


It is used in separating gel mix for SDS- PAGE.






100ml

250ml

500ml

990.00

1800.00

3450.00

OP-5016-100ml

OP-5016-250ml

OP-5016-500ml


It used as a wash buffer and antibody diluent for ELISA, Western blotting and other immunoassays.

Pro CheMol - Tris buffer saline (10X) (TBS)





100ml

250ml

500ml

490.00

1200.00

1990.00

OP-5017-100ml

OP-5017-250ml

OP-5017-500ml


It used as a wash buffer and antibody diluent for ELISA, Western blotting and other immunoassays.

Pro CheMol - Tris buffer saline Tween-20 (10X ) (TBST)





100ml

250ml

500ml

550.00

1300.00

2490.00

OP-5018-100ml

OP-5018-250ml

OP-5018-500ml


WBC lysis buffer used in whole blood DNA isolation ,followed by RBC lysis buffer, to increase the nucleic acid yield.

Pro CheMol - WBC Lysis Buffer




Pro CheMol a- WBC Lysis Buffer

100ml

250ml

500ml

690.00

1590.00

2900.00

OP-5019-100ml

OP-5019-250ml

OP-5019-500ml


Our Blood DNA Lysis Buffer is designed for the optimal lysis of erythrocytes, while having minimal effect of lymphocytes, when used as directed. The use of Lysis Buffer allows for the preferential lysis of red blood cells from whole blood and as these are the majority of cells in whole blood permits the concentration of the nucleated white blood cells. This buffer is ideal for the isolation of DNA and RNA from blood.

Pro CheMol- Whole blood DNA lysis buffer


128

BUFF

ERS

PR

OD

UC

T C

ATA

LOG

UE

It used in Blotting techniques to transfer the Bands from gel to membrane.

Pro CheMol- Blotting Buffer





100ml

250ml

500ml

600.00

990.00

1300.00

OP-5101 -100ml

OP-5101 -250ml

OP-5101 -500ml


It is used as tank buffer for immune electrophoresis techniques.

Pro CheMol- Immuno Buffer (20X)





100ml

250ml

500ml

2000.00

3000.00

4250.00

OP-5102 -100ml

OP-5102 -250ml

OP-5102 -500ml


MOPS buffer used as running buffer in Northern blotting. MOPS are the common name for the compound 3-(N-morpholino) propansulfonic acid, a buffer introduced by Good et al. in the 1960s. It is a structural analog to MES. Its chemical structure contains a morpholine ring. 10X MOPS Buffer is intended for use at 1X as the electrophoresis and gel running buffer during the separation of RNA on denaturing formalde-hyde/agarose gels.





50ml

100ml

1250.00

2000.00

OP-5103 -50ml

OP-5103 -100ml

Storage : 4°C

A hybridization buffer for Northern and southern blots.

Pro CheMol- SSPE buffer (20X)





100ml

250ml

500ml

500.00

750.00

1400.00

OP-5104 -100ml

OP-5104 -250ml

OP-5104 -500ml

Storage : 4°C





100ml

250ml

500ml

540.00

1200.00

2150.00

OP-5020-100ml

OP-5020-250ml

OP-5020-500ml

ELECTROPHORESIS BUFFER

129

BUFF

ERS

PR

OD

UC

T C

ATA

LOG

UE

It is used as running buffer in agarose gel electrophoresis.

Pro CheMol- TAE (10X)





100ml

250ml

500ml

420.00

600.00

1100.00

OP-5105 -100ml

OP-5105 -250ml

OP-5105 -500ml


It is used as running buffer in agarose gel electrophoresis.






100ml

250ml

500ml

1200.00

1600.00

3000.00

OP-5106 -100ml

OP-5106 -250ml

OP-5106 -500ml


TEB is an abbreviation of Tris EDTA Boric acid and it is used in Agarose Gel Electrophoresis.

Pro CheMol- TEB (10X)





100ml

250ml

500ml

600.00

1000.00

1900.00

OP-5107 -100ml

OP-5107 -250ml

OP-5107 -500ml


In NATIVE - PAGE used as Tank Buffer.

Pro CheMol - Tris - Glycine Buffer for NATIVE - PAGE (10X)





100ml

250ml

500ml

500.00

1000.00

1300.00

OP-5108 -100ml

OP-5108 -250ml

OP-5108 -500ml


In SDS - PAGE used as Running Buffer.

Pro CheMol - Tris - Glycine - SDS Buffer for SDS - PAGE (10X)





100ml

250ml

500ml

500.00

800.00

1300.00

OP-5109 -100ml

OP-5109 -250ml

OP-5109 -500ml


This buffer used to make Tris- Tricine Gel.

Pro CheMol - Tris - Tricine Buffer (10X)


Pro CheMol - Tris -Tricine Buffer (10X)



100ml

250ml

500ml

1100.00

2300.00

4000.00

OP-5110 -100ml

OP-5110 -250ml

OP-5110 -500ml


130

CONT

ENTS

Laboratory Reagents Pro CheMol-Acrylamide / Bis- Acrylamide -30% (28 : 2) 131

Pro CheMol-Acrylamide / Bis- Acrylamide -30% (29 : 1) 131

Pro CheMol-Acrylamide / Bis- Acrylamide -35% (34 : 1) 131

Pro CheMol-Amidoblock Stain 131

Pro CheMol-Andrade's Indicator 131

Pro CheMol-Baker's Softening Fluid (Softening of Animal Tissue) 132

Pro CheMol-Barfoeds reagent 132

Pro CheMol-Benedicts Qualitative Solution 132

Pro CheMol-Bovine Serum Albumin solution (10%) 132

Pro CheMol-Bradfords reagent 132

Pro CheMol-CBB-G250 Stainer (4X) 133

Pro CheMol-CBB-R250 Stainer (4X) 133

Pro CheMol-Colloidal Protein staining solution 133

Pro CheMol-Crystal violet (Gram Stain) solution 133

Pro CheMol-Crystal Violet solution 133

Pro CheMol-Cyanomethemoglobin (20mg /ml) 134

Pro CheMol-Destainer 134

Pro CheMol-Denhardt's solution (50X) 134

Pro CheMol-Diphenylamine Reagent 134

Pro CheMol-DTT -Dithiothreitol solution (1M) 134

Pro CheMol-Drabkin's Reagent 134

Pro CheMol-EDTA -0.5M 135

Pro CheMol-Eosin Stain 135

Pro CheMol-Ethidium Bromide (10mg / ml) 135

Pro CheMol-Fehling's A & B Solution 135

Pro CheMol-Folin's reagent 135

Pro CheMol-Gel Loading Dye (6X) 135

Pro CheMol-Giemsa Stain 136

Pro CheMol-Glycerol (50%) 136

Pro CheMol-Iodine solution (0.05M) 136

Pro CheMol-Iodine Solution ( Gram Stain) 136

Pro CheMol-Iodine-Potassium iodide solution (For Starch Test) 136

Pro CheMol-Kovac's Reagent 137

Pro CheMol-Malachite Green Solution 137

Pro CheMol-Neutral Red Solution 137

Pro CheMol-Normal Saline 137

Pro CheMol-Orange Gel Loading Dye (6X) 137

Pro CheMol-pH Standard 10.0 137



Pro CheMol-Phenol : Chloroform : Isoamylalcohol (25:24:1) 138

Pro CheMol-Phenol Tris Saturated 138

Pro CheMol-Phenol water Saturated 138

Pro CheMol-Phenol:Chloroform 139

Pro CheMol-Ponceau's Stain (2X) 139

Pro CheMol-Protein Standard (BSA 1mg/ml) 139

Pro CheMol-RBC Diluting Fluid 139

Pro CheMol-Ringer's Solution 139

Pro CheMol-RNA later Solution 140

Pro CheMol-5X Sample Solubilizing Buffer for NATIVE -PAGE 140

Pro CheMol-5X Sample Solubilizing Buffer for SDS -PAGE 140

Pro CheMol-SDS Solution 10% 140

Pro CheMol-Seliwanoffs reagent 140

Pro CheMol-Separating gel mix 140

Pro CheMol-Sodium Acetate (5M) 141

Pro CheMol-Stacking Gel Mix (4%) 141

Pro CheMol-Starch Standard(10mg/ml) 141

Pro CheMol-Trizol 141

Pro CheMol-Trypan Blue solution 0.4% 141

Pro CheMol-VP Reagent 142

Pro CheMol-Water (DEPC Treated) 142

Pro CheMol-WBC Diluting Fluid 142

Reagents For PCR 100mM dATP Solution 143

100mM dCTP Solution 143

100mM dGTP Solution 143

100mM dNTP Mix Solution 143

100mM dTTP Solution 143

10mM dNTP Mix Solution 143

10X PCR Buffer 143

MgCl2 (25mM) for PCR 143

M-MuLV Reverse Transcriptase 143

PCR Master Mix with Taq Polymerase 143

PCR water 143

Taq Polymerase 143

REAGENTS

131

REAG

ENTS

PR

OD

UC

T C

ATA

LOG

UE

REAGENTSLABORATORY REAGENTS

It used in SDS-PAGE

Pro CheMol -Acrylamide / Bis- Acrylamide -30% (28: 2)





100ml

250ml

500ml

1200.00

2490.00

4150.00

OP-5201-100ml

OP-5201-250ml

OP-5201-500ml

Storage : 4°C

For the sugar fermentation tests, the sugar media will be pinkish in color before inoculation. After inoculation: Under anaerobic conditions, the anaerobic organisms will break down the sugars to produce lactic acid thus changing the pH of the medium (more acidic = lesser than 7). At this point, the Andrade's indicator changes from pink to yellow, depending on how acidic it is; the more acid it is, the deeper yellow color it will present.

Pro CheMol -Andrade's Indicator





100ml

250ml

500ml

740.00

1650.00

3190.00

OP-5205-100ml

OP-5205-250ml

OP-5205-500ml

Storage : RT

It used in SDS-PAGE






100ml

250ml

500ml

1250.00

2530.00

4190.00

OP-5202-100ml

OP-5202-250ml

OP-5202-500ml

Storage : 4°C

It used in SDS-PAGE






100ml

250ml

500ml

1370.00

2690.00

4280.00

OP-5203-100ml

OP-5203-250ml

OP-5203-500ml

Storage : 4°C

It is a diazo dye used in biochemical research to stain for total protein on transferred membrane blots. It is also used in criminal investigations to detect blood present with latent �ngerprints.

Pro CheMol -Amidoblack Stain





100ml

250ml

500ml

900.00

1500.00

2950.00

OP-5204-100ml

OP-5204-250ml

OP-5204-500ml

Storage : RT

132

REAG

ENTS

PR

OD

UC

T C

ATA

LOG

UE

Softening of Animal Tissue

Pro CheMol -Baker's Softening Fluid





100ml

250ml

500ml

1750.00

4220.00

7650.00

OP-5206-100ml

OP-5206-250ml

OP-5206-500ml

Storage : RT

Barfoed's test is a chemical test used for detecting the presence of monosaccharides. It is based on the reduction of copper (II) acetate to copper (I) oxide (Cu

2O), which forms a brick-red precipitate.

Pro CheMol -Barfoed reagent





100ml

250ml

500ml

890.00

2050.00

3190.00

OP-5207-100ml

OP-5207-250ml

OP-5207-500ml

Storage : RT

Commonly used to detect presence of reducing sugar(s)

Pro CheMol -Benedicts Qualitative Solution





100ml

250ml

500ml

780.00

1820.00

2790.00

OP-5208-100ml

OP-5208-250ml

OP-5208-500ml

Storage : RT

Used to determine the concentration of proteins in solution. The procedure is based on the formation of a complex between the dye, Brilliant Blue G, and proteins in solution.

Pro CheMol -Bradford’s reagent





100ml

250ml

500ml

3390.00

6850.00

12500.00

OP-5210-100ml

OP-5210-250ml

OP-5210-500ml

Storage : RT

It used in Research

Pro CheMol - Bovine serum Albumin solution (10%)





50ml

100ml

250ml

2070.00

3750.00

8700.00

OP-5209-50ml

OP-5209-100ml

OP-5209-250ml

Storage : 4°C

133

REAG

ENTS

PR

OD

UC

T C

ATA

LOG

UE

For detection of protein bands following electrophoresis.

Pro CheMol -CBB-G250 Stainer (4X)





100ml

250ml

500ml

690.00

990.00

1500.00

OP-5211-100ml

OP-5211-250ml

OP-5211-500ml

Storage : RT

It used for the quantitative, colorimetric determination of hemoglobin concentration in whole blood at 540 nm.

Pro CheMol -Cyanomethemoglobin (20mg /ml)

Storage : 4°C

R-250 (red-tinted) to estimate protein by Bradford method.

Pro CheMol -CBB-R250 Stainer (4X)





100ml

250ml

500ml

700.00

1090.00

1600.00

OP-5212-100ml

OP-5212-250ml

OP-5212-500ml

Storage : RT

The Colloidal Blue Staining Kit allows detection of nanogram levels of protein with minimal effort and requires only water to destain.

Pro CheMol -Colloidal Protein staining solution





100ml

250ml

500ml

2700.00

6390.00

11890.00

OP-5213-100ml

OP-5213-250ml

OP-5213-500ml

Storage : RT

The dye is used as a histological stain and in Gram's method of classifying bacteria

Pro CheMol -Crystal violet (Gram Stain) solution





100ml

250ml

500ml

1150.00

2690.00

5150.00

OP-5215-100ml

OP-5215-250ml

OP-5215-500ml

Storage : RT

When conducting DNA gel electrophoresis, crystal violet can be used as a non-toxic DNA stain as an alternative to �uorescent, intercalating dyes such as ethidium bromide.

Pro CheMol -Crystal Violet solution





100ml

250ml

500ml

850.00

1890.00

3200.00

OP-5216-100ml

OP-5216-250ml

OP-5216-500ml

Storage : RT

134

REAG

ENTS

PR

OD

UC

T C

ATA

LOG

UE





100ml

250ml

500ml

1490.00

3150.00

5390.00

OP-5217-100ml

OP-5217-250ml

OP-5217-500ml

Denhardt's Solution 50X is a mixture of blocking agents used in membrane-based hybridization protocols. The solution contains Ficoll (type 400)- 2% (w/v), polyvinylpyrrolidone 2% (w/v), and bovine serum albumin 2% (w/v). Sterile �ltered. It used as a blocking agent routinely used to reduce non-speci�c binding of detection reagents to the Northern and Southern Blot membranes. This powder can be used to make a 50x concentrate that can be diluted to any concentration for use in a variety of pre-hybridization and hybridization buffers.

Pro CheMol -Denhardt's solution (50X)

Storage : RT





100ml

250ml

500ml

4850.00

9500.00

18950.00

OP-5218-100ml

OP-5218-250ml

OP-5218-500ml

It used for quantitative analysis of DNA

Pro CheMol -Diphenylamine Reagent

Storage : RT





100ml

250ml

500ml

1200.00

2300.00

3900.00

OP-5219-100ml

OP-5219-250ml

OP-5219-500ml

It used for the quantitative, colorimetric determination of hemoglobin.

Pro CheMol - Drabkin’s Reagent

Storage : RT





100ml

250ml

500ml

1450.00

3090.00

5250.00

OP-5221-100ml

OP-5221-250ml

OP-5221-500ml

Dithiothreitol is an effective reducing agent for protein analysis.

Pro CheMol -DTT-Dithiothreitol solution (1M)

Storage : 4°C




1ml

1mlX5Nos

750.00

2990.00

OP-5220-1ml

OP-5220-5ml

It is used to destain the gels

Pro CheMol – Destainer

Storage : RT





100ml

250ml

500ml

380.00

750.00

1200.00

OP-5263-100ml

OP-5263-250ml

OP-5263-500ml

135

REAG

ENTS

PR

OD

UC

T C

ATA

LOG

UE

It is widely used to dissolve limescale. Its usefulness arises because of its role as a hexadentate ("six-toothed") ligand and chelating agent, i.e., its ability to "sequester" metal ions such as Ca2+ and Fe3+.

Pro CheMol -EDTA -0.5M

Storage : RT





100ml

250ml

500ml

770.00

1590.00

2650.00

OP-5222-100ml

OP-5222-250ml

OP-5222-500ml

Eosin is a �uorescent acidic / negative compound that binds to and forms salts with basic, or eosinophilic, compounds containing positive charges and stains them dark red or pink as a result of the actions of bromine on �uorescein.

Pro CheMol -Eosin Stain

Storage : RT





100ml

250ml

500ml

1350.00

3190.00

5350.00

OP-5223-100ml

OP-5223-250ml

OP-5223-500ml

It used to differentiate between water-soluble carbohydrate and ketone functional groups, and as a test for monosaccharides.

Pro CheMol -Fehling's A & B Solution

Storage : RT





100ml

250ml

500ml

630.00

1180.00

1990.00

OP-5225-100ml

OP-5225-250ml

OP-5225-500ml

It used to measure levels of amines and amino acids. The reagent produces a bright red color in alkaline solutions and is also �uorescent.

Pro CheMol - Folin’s reagent

Storage : RT




100ml

250ml

1790.00

3990.00

OP-5226-100ml

OP-5226-250ml

Ethidium bromide is an intercalating agent commonly used as a �uorescent tag in molecular biology.

Pro CheMol -Ethidium Bromide (10mg / ml)

Storage : RT




1ml

1mlX5Nos

960.00

3650.00

OP-5224-1ml

OP-5224-5ml

Gel Loading Dye, Blue (6X) is a pre-mixed loading buffer with one tracking dye for agarose and non-denaturing polyacrylamide gel electro-phoresis

Pro CheMol -Gel Loading Dye (6X)

Storage : RT




1ml

1mlX6Nos

250.00

950.00

OP-5227-1ml

OP-5227-6ml

REAG

ENTS

136

PR

OD

UC

T C

ATA

LOG

UE

It is speci�c for the phosphate groups of DNA and attaches itself to regions of DNA where there are high amounts of adenine-thymine bonding.

Pro CheMol -Giemsa Stain

Storage : RT





100ml

250ml

500ml

590.00

1180.00

1990.00

OP-5228-100ml

OP-5228-250ml

OP-5228-500ml

It is a Storage System for Bacterial culture (Sterilized).

Pro CheMol -Glycerol (50%)

Storage : RT




50ml

100ml

1250.00

2350.00

OP-5229-50ml

OP-5229-100ml

This solution is used as an indicator test for the presence of starches in organic compounds, with which it reacts by turning a dark-blue/-black.

Pro CheMol -Iodine solution (0.05M)

Storage : RT





100ml

250ml

500ml

1170.00

2550.00

4690.00

OP-5230-100ml

OP-5230-250ml

OP-5230-500ml

As a mordant when performing a Gram stain. It is applied for 1 minute after staining with crystal violet, but before ethanol to ensure that gram positive organism’s peptidoglycan remains stained, easily identifying it as a gram positive in microscopy.

Pro CheMol -Iodine Solution ( Gram Stain)

Storage : RT





100ml

250ml

500ml

950.00

2350.00

4190.00

OP-5231-100ml

OP-5231-250ml

OP-5231-500ml

The iodine/ potassium iodide solution is used as an indicator for starch.

Pro CheMol -Iodine-Potassium iodide solution (For Starch Test)

Storage : RT





100ml

250ml

500ml

1550.00

3650.00

6900.00

OP-5232-100ml

OP-5232-250ml

OP-5232-500ml

It is used for the diagnostical indole test, to determine the ability of the organism to split indole from the amino acid tryptophan

Pro CheMol -Kovac's Reagent

Storage : RT

137

REAG

ENTS

PR

OD

UC

T C

ATA

LOG

UE





100ml

250ml

500ml

1850.00

3800.00

6290.00

OP-5233-100ml

OP-5233-250ml

OP-5233-500ml

Intended for use with Carbol Fuchsin in Acid Fast Staining, Procedure.

Pro CheMol -Malachite Green Solution

Storage : RT





100ml

250ml

500ml

450.00

790.00

1150.00

OP-5234-100ml

OP-5234-250ml

OP-5234-500ml

This dye used for staining in histology. It stains lysosomes red. It is used as a general stain in histology, as a counterstain in combination with other dyes, and for many staining methods.

Pro CheMol -Neutral Red Solution

Storage : RT





100ml

250ml

500ml

790.00

1450.00

2400.00

OP-5235-100ml

OP-5235-250ml

OP-5235-500ml

Normal saline is a sterile mixture of salt and water. It is called normal because it’s salt concentration is similar to tears, blood, and other body �uids (0.9% saline). It is also called isotonic solution.

Pro CheMol -Normal Saline (10X)

Storage : RT





100ml

250ml

500ml

250.00

400.00

590.00

OP-5236-100ml

OP-5236-250ml

OP-5236-500ml

It used for pH calibration at pH 10.00

Pro CheMol -pH Standard 10.0

Storage : RT





100ml

250ml

500ml

140.00

190.00

250.00

OP-5238-100ml

OP-5238-250ml

OP-5238-500ml

It contains two different dyes (xylene cyanol FF and orange G) for visual tracking of DNA migration during electrophoresis.

Pro CheMol -Orange Gel Loading Dye (6X)

Storage : RT




1ml

1mlX6Nos

240.00

950.00

OP-5237-1ml

OP-5237-6ml

REAG

ENTS

138

PR

OD

UC

T C

ATA

LOG

UE



Storage : RT





100ml

250ml

500ml

140.00

190.00

250.00

OP-5239-100ml

OP-5239-250ml

OP-5239-500ml



Storage : RT





100ml

250ml

500ml

140.00

190.00

250.00

OP-5240-100ml

OP-5240-250ml

OP-5240-500ml

For applications requiring a higher pH, such as the isolation of large intact genomic DNA, addition of the Equilibration Buffer is recommended.

Pro CheMol -Phenol: Chloroform: Isoamylalcohol (25:24:1)

Storage : 4°C





100ml

250ml

500ml

2650.00

5600.00

7800.00

OP-5241-100ml

OP-5241-250ml

OP-5241-500ml

It used in DNA or RNA extraction

Pro CheMol -Phenol Tris Saturated

Storage : 4°C





50ml

100ml

250ml

1090.00

1950.00

4200.00

OP-5242-50ml

OP-5242-100ml

OP-5242-250ml

Water Saturated Phenol (pH 6.6) is used in RNA and DNA extraction procedures.

Pro CheMol -Phenol Water Saturated

Storage : 4°C

It used in DNA or RNA extraction

Pro CheMol -Phenol: Chloroform

Storage : 4°C





50ml

100ml

250ml

1050.00

1890.00

4050.00

OP-5243-50ml

OP-5243-100ml

OP-5243-250ml

139

REAG

ENTS

PR

OD

UC

T C

ATA

LOG

UE





100ml

250ml

500ml

2330.00

4600.00

8000.00

OP-5244-100ml

OP-5244-250ml

OP-5244-500ml

Ponceau’s dye is used to make a stain for rapid reversible staining of protein bands on nitrocellulose or PVDF membranes (Western blots) and also for staining protein on cellulose acetate membranes.

Pro CheMol - Ponceau's Stain (2X)

Storage : RT





100ml

250ml

500ml

850.00

1900.00

3600.00

OP-5245-100ml

OP-5245-250ml

OP-5245-500ml

It used to dilute the RBC content to enhance the counting of cells.

Pro CheMol - RBC Diluting Fluid

Storage : RT





100ml

250ml

500ml

590.00

980.00

1490.00

OP-5247-100ml

OP-5247-250ml

OP-5247-500ml

Ringer's solution is frequently used in in vitro experiments on organs or tissues, such as in vitro muscle testing.

Pro CheMol - Ringer's Solution

Storage : RT





100ml

250ml

500ml

470.00

750.00

1090.00

OP-5248-100ml

OP-5248-250ml

OP-5248-500ml

RNA later is an aqueous, non-toxic tissue and cell storage reagent that stabilizes and protects cellular RNA in intact, unfrozen tissue and cell samples. RNA later eliminates the need to immediately process samples or to freeze samples in liquid nitrogen for later processing. RNA later can be used with various downstream applications including mRNA and total RNA isolation, histology and immunocytochemistry RNA later is easy to use. Simply cut tissue samples to be stored so they are less than 0.5 cm in at least one dimension and submerge in 5 volumes of RNA later. Small organs, such as rat kidney, liver or spleen can be stored in whole in RNA later. When ready to isolate the RNA, remove the tissue from RNA later and process as though just harvested. For cell storage, resuspend pelleted cells in a small amount of PBS before adding 5-10 volumes of RNA later. Before preparing RNA, pellet cells and discard supernatant.

Pro CheMol - RNA Later Solution

Storage : RT

It used as a standard for estimation of proteins.

Pro CheMol - Protein Standard (BSA 1mg/ml)

Storage : 4°C





10ml

50ml

100ml

1190.00

2290.00

4150.00

OP-5246-10ml

OP-5246-50ml

OP-5246-100ml

REAG

ENTS

140

PR

OD

UC

T C

ATA

LOG

UE





100ml

250ml

500ml

5220.00

12420.00

19670.00

OP-5249-100ml

OP-5249-250ml

OP-5249-500ml

Native protein sample buffer (without SDS) is added to solubilized protein samples prior to analysis by SDS-Polyacrylamide gel electrophore-sis (SDS-PAGE). 1 part of this 5X buffer is added to 4 Parts solubilized protein

Pro CheMol - 5X Sample Solubilizing Buffer (NATIVE - PAGE)

Storage : RT




1ml

1mlX5Nos

350.00

1350.00

OP-5250-1ml

OP-5250-5ml

SDS protein sample buffer is added to solubilized protein samples prior to analysis by SDS-polyacrylamide gel electrophoresis (SDS-PAGE). 1 part of this 5X buffer is added to 4 parts solubilized protein.

Pro CheMol - 5X Sample Solubilizing Buffer (SDS - PAGE)

Storage : RT




1ml

1mlX5Nos

370.00

1370.00

OP-5251-1ml

OP-5251-5ml

It’s a denaturant of protein sample (SDS-PAGE)

Pro CheMol – SDS Solution (10%)

Storage : RT





100ml

250ml

500ml

880.00

1500.00

2800.00

OP-5252-100ml

OP-5252-250ml

OP-5252-500ml

Seliwanoff’s test is a chemical test which distinguishes between aldose and ketose sugars.

Pro CheMol – Seliwanoff’s reagent

Storage : RT





100ml

250ml

500ml

980.00

2150.00

3970.00

OP-5253-100ml

OP-5253-250ml

OP-5253-500ml

It used for SDS-PAGE (Separating Gel formation)

Pro CheMol – Separating gel mix

Storage : 4°C





100ml

250ml

500ml

1500.00

3450.00

6050.00

OP-5254-100ml

OP-5254-250ml

OP-5254-500ml

141

REAG

ENTS

PR

OD

UC

T C

ATA

LOG

UE

It can use as a neutralization buffer in plasmid isolation.

Pro CheMol – Sodium Acetate (5M)

Storage : RT





100ml

250ml

500ml

1450.00

3400.00

6200.00

OP-5255-100ml

OP-5255-250ml

OP-5255-500ml

Used for SDS-PAGE (Stacking Gel formation)

Pro CheMol – Stacking Gel Mix (4%)

Storage : 4°C





100ml

250ml

500ml

890.00

1980.00

3490.00

OP-5256-100ml

OP-5256-250ml

OP-5256-500ml

This stain used in animail tissue culture technique.

Pro CheMol – Trypan Blue solution 0.4%

Storage : RT





100ml

250ml

500ml

450.00

890.00

1490.00

OP-5259-100ml

OP-5259-250ml

OP-5259-500ml

This is often used in chemistry as an indicator for redox titrations where tri iodide is present.

Pro CheMol – Starch Standard (10mg/ml)

Storage : 4°C





10ml

50ml

100ml

390.00

1290.00

2190.00

OP-5257-10ml

OP-5257-50ml

OP-5257-100ml

It used for isolation of RNA

Pro CheMol – Trizol

Storage : 4°C





20ml

50ml

100ml

3400.00

8300.00

16300.00

OP-5258-20ml

OP-5258-50ml

OP-5258-100ml

REAG

ENTS

142

PR

OD

UC

T C

ATA

LOG

UE


Pro CheMol – VP Reagent



100ml

250ml

500ml

1140.00

2670.00

5140.00

OP-5260-100ml

OP-5260-250ml

OP-5260-500ml

It used to inactivate RNase enzymes in water and in laboratory.

Pro CheMol – Water -DEPC Treated

Storage : RT





100ml

250ml

500ml

390.00

810.00

1550.00

OP-5261-100ml

OP-5261-250ml

OP-5261-500ml

Dilute the WBC content to enhance the counting of cells.

Pro CheMol – WBC Diluting Fluid (Turk's �uid)

Storage : RT





100ml

250ml

500ml

950.00

2130.00

3890.00

OP-5262-100ml

OP-5262-250ml

OP-5262-500ml

Reagent used to detect acetoin in a bacterial broth culture


Storage : RT

143

PCR

REAG

ENTS

PR

OD

UC

T C

ATA

LOG

UE

PCR ReagentsCat.No Product Volume Price (`)

100mM dATP Solution

1800.00

3070.00

12280.00

1800.00

3070.00

12280.00

1800.00

3070.00

12280.00

2330.00

4660.00

11650.00

1800.00

3070.00

12280.00

1200.00

2100.00

4860.00

1200.00

2000.00

4800.00

200.00

450.00

900.00

2400.00

9900.00

2200.00

9450.00

18000.00

220.00

1000.00

1700.00

P.O.R*

P.O.R*

P.O.R*

OP-5301-100µl

OP-5301-200µl

OP-5301-1ml

100µl

200µl

1ml

100mM dCTP Solution

OP-5302-100µl

OP-5302-200µl

OP-5302-1ml

100µl

200µl

1ml

100mM dGTP Solution

OP-5303-100µl

OP-5303-200µl

OP-5303-1ml

100µl

200µl

1ml

100mM dNTP Mix Solution

OP-5304-100µl

OP-5304-200µl

OP-5304-1ml

100µl

200µl

1ml

100mM dTTP Solution

OP-5305-100µl

OP-5305-200µl

OP-5305-1ml

100µl

200µl

1ml

10mM dNTP Mix Solution

OP-5306-100µl

OP-5306-200µl

OP-5306-1ml

100µl

200µl

1ml

10X PCR Buffer

OP-5307-1ml

OP-5307-2ml

OP-5307-5ml

1ml

2ml

5ml

PCR water

OP-5311-1ml

OP-5311-5ml

OP-5311-10ml

1ml

5ml

10ml

MgCl2 (25mM) For PCR

OP-5308-1ml

OP-5308-2ml

OP-5308-5ml

1ml

2ml

5ml

M-MuLV Reverse TranscriptaseOP-5309-1KU

OP-5309-5KU

1000U

5000U

PCR Master Mix with Taq polymerase

OP-5310-100µl

OP-5310-500µl

OP-5310-1ml

100µl

500µl

1ml

Taq Polymerase

OP-5312-100U

OP-5312-250U

OP-5312-500U

100U

250U

500U

*Price on Request

144

CONT

ENTS

Acrylamide 145

Agarose Low EEO 145

Agarose High EEO 145

Ampicillin(Sodium salt) 145

Bisacrylamide 145

2-b-Mercaptoethanol (BME) 146

Bromophenol Blue (BPB) 146

Bovine Serum Albumin (BSA) 146

Coomassie Brilliant Blue G-250 (CBB-G250) 146

Coomassie Brilliant Blue R-250 (CBB-R250) 147

Cetyltrimethyl Ammonium Bromide (C-TAB) 147

Diethylpyrocarbonate (DEPC) 147

Dimethyl Sulphoxide (DMSO 147

Ethylenediaminetetraacetic acid (EDTA) 148

Ethidium Bromide 148

Formamide 148

Guanidine hydrochloride 148

IPTG 148

NP-40 149

Phenol 149

Polyvinylpyrrolidone (PVP) 149

SDS 149

Sodium acetate 150

TEMED (Tetramethylethylenediamine) 150

Tris Hcl 150

Tris Buffer 150

Triton-X-100 151

X-Gal 151

Strains & Immunocomponents 152

Lab Wares 153

BIOCHEMICALS

145

BIOC

HEM

ICAL

SP

RO

DU

CT

CAT

ALO

GU

E

BIOCHEMICALS

Acrylamide

Cat.No Product Quantity Price (`)

Acrylamide

Acrylamide

Acrylamide

50g

100g

500g

1640.00

3070.00

14350.00

OP-5401-50g

OP-5401-100g

OP-5401-500g

CAS Number : 79-06-1

Molecular Formula : CH2=CHCONH

2

Molecular Weight : 71.08

Agarose LOW EEO


Agarose LOW EEO

Agarose LOW EEO

Agarose LOW EEO

10g

25g

50g

2700.00

5280.00

10000.00

OP-5402-10g

OP-5402-25g

OP-5402-50g


Form : Powder

Color : White to off-white

Agarose High EEO


Agarose High EEO

Agarose High EEO

Agarose High EEO

10g

25g

50g

2860.00

5610.00

10000.00

OP-5403-10g

OP-5403-25g

OP-5403-50g


Form : Powder

Color : White to off-white

Ampicillin (Sodium Salt)





1g

5g

10g

1175.00

4260.00

7460.00

OP-5404-1g

OP-5404-5g

OP-5404-10g

D-(−)-α-Aminobenzylpenicillin sodium salt

Empirical Formula : C16

H18

N3NaO

4S



Bisacrylamide

N,N’-Bisacryloyl-1,2-dihydroxy-1,2ethylenediamine,

Formula : [H2C=CHCONHCH(OH)-]

2



146

BIOC

HEM

ICAL

SP

RO

DU

CT

CAT

ALO

GU

ECat.No Product Quantity Price (`)

Bisacrylamide

Bisacrylamide

Bisacrylamide

10g

50g

100g

990.00

4120.00

6580.00

OP-5405-10g

OP-5405-50g

OP-5405-100g


2-β-Mercaptoethanol (BME)



50ml

100ml

250ml

1550.00

2810.00

5880.00

OP-5406-50ml

OP-5406-100ml

OP-5406-250ml

2−β−Mercaptoethanol (BME)

Chemical Formula : C2H

6SO

Molar Mass : 78.133 g mol−1

Density : 1.114 g cm−3

Melting Point : −100 °C (−148 °F; 173 K)

Boiling Point : 157 °C; 314 °F; 430 K


Bromophenol Blue (BPB)



1g

5g

10g

680.00

2090.00

3160.00

OP-5407-1g

OP-5407-5g

OP-5407-10g


Molecular Formula : C19

H10

Br4O

5S


Loss On Drying : max 1%

A 1%,1cm at 436nm,pH 3.0


Bovine serum Albumin (BSA)



1g

2g

5g

1050.00

1920.00

4670.00

OP-5408-1g

OP-5408-2g

OP-5408-5g


Number of amino acid residues : 583

Molecular Weight : 66,463 Da (= 66.5 kDa)

Isoelectric point in water at 25°C : 4.7


Coomassie Brilliant Blue G-250 (CBB-G250)



1g

5g

10g

650.00

3190.00

4780.00

OP-5409-1g

OP-5409-5g

OP-5409-10g


Chemical Formula : C47

H50

N3NaO

7S

2 (Sodium salt)

Molar Mass : 856.03 g/mol

Solubility in Water : Slightly soluble in cold, soluble in hot (bright blue)

Solubility in Ethanol : Soluble

147

BIOC

HEM

ICAL

SP

RO

DU

CT

CAT

ALO

GU

E


Coomassie Brilliant Blue R-250 (CBB-R250)



1g

5g

10g

610.00

2800.00

4180.00

OP-5410-1g

OP-5410-5g

OP-5410-10g



H44

N3NaO

7S

2 (Sodium salt)

Molar Mass : 825.97g/mol

Solubility in Water : Insoluble in cold, slightly soluble in hot (bright red blue)

Solubility in Ethanol : Slightly Soluble


Cetyltrimethyl ammonium Bromide (C-TAB)



25g

50g

100g

1210.00

1930.00

2850.00

OP-5411-25g

OP-5411-50g

OP-5411-100g



H42

BrN


Appearance : White Powder

Melting Point : 237 to 243°C (459 to 469°F)


Diethylpyrocarbonate (DEPC)



1ml

5ml

25ml

880.00

2140.00

8910.00

OP-5412-1ml

OP-5412-5ml

OP-5412-25ml


Chemical Formula : C6H

10O

5

Molar Mass : 162.14 g.mol−1

Appearance : Clear, colorless liquid

Density : 1.101 g/mL at 25 °C / 1.121 g/mL at 20 °C

Boiling Point : 93 to 94 °C (199 to 201°F; 366 to 367K) at 24 hPa


Dimethyl Sulphoxide (DMSO)



100ml

200ml

500ml

1750.00

2730.00

5580.00

OP-5413-100ml

OP-5413-200ml

OP-5413-500ml


Molecular Formula : C2H

6OS

Molar Mass : 78.13 g/mol-1

Appearance : Colourless liquid

Density : 1.1004 g cm-3

Melting point : 19 °C (66 °F; 292 K)

Boiling Point : 189 °C (372 °F; 462 K)

Solubility in water : Miscible

Solubility in diethyl ether : Very soluble

148

BIOC

HEM

ICAL

SP

RO

DU

CT

CAT

ALO

GU

E


Ethylenediaminetetraacetic acid (EDTA)



100g

200g

500g

2420.00

4450.00

8690.00

OP-5414-100g

OP-5414-200g

OP-5414-500g


Formula : C10

H16

N2O

8


IUPAC ID : 2,2',2'',2'''-(Ethane-1,2-diyldinitrilo) tetraacetic acid

Melting point : 237 °C

Density : 860.00 kg/m³

Soluble in : Water


Ethidium Bromide

Ethidium Bromide

Ethidium Bromide

10mg

50mg

100mg

2420.00

4450.00

8690.00

OP-5415-10mg

OP-5415-50mg

OP-5415-100mg

Ethidium Bromide

Formula : C21

H20BrN

3



Formamide

Formamide

Formamide

100ml

200ml

500ml

1930.00

2660.00

5580.00

OP-5416-100ml

OP-5416-200ml

OP-5416-500ml

Formamide

Boiling point : 210 °C

Formula : CH3NO

Density : 1.13 g/cm³



Guanidine hydrochloride



50g

100g

200g

2690.00

4510.00

7920.00

OP-5417-50g

OP-5417-100g

OP-5417-200g


IPTG - Isopropyl β-D-1-thiogalactopyranoside



1g

5g

10g

400.00

1800.00

3560.00

OP-5418-1g

OP-5418-5g

OP-5418-10g




Molecular Formula : CH6ClN

3

CAS NO : 50-01-1



18O

5S

Molar Mass : 238.30 g mol-1

149

BIOC

HEM

ICAL

SP

RO

DU

CT

CAT

ALO

GU

E


NP-40

NP-40

NP-40

10ml

50ml

100ml

920.00

3570.00

6050.00

OP-5419-10ml

OP-5419-50ml

OP-5419-100ml

NP-40

NP-40 : A nonionic detergent

Molecular Weight : 617


Phenol

Phenol

250g

500g

1900.00

3686.00

OP-5420-250g

OP-5420-500g

Phenol

Formula : C6H

6O



Boiling Point : 181.7 °C

Melting Point : 40.5 °C

IUPAC ID : Phenol

Soluble in : Water

Polyvinylpyrrolidone (PVP)

Chemical Formula : (C6H

9NO)

n

Molar Mass : 2.500 – 2.500.000 g.mol−1

Appearance : White to light yellow, hygroscopic, amorphous powder

Density : 1.2 g/cm3

Melting point : 150 to 180 °C (302 to 356 °F; 423 to 453 K) (glass temperature)





50g

100g

250g

1750.00

2860.00

5390.00

OP-5421-50g

OP-5421-100g

OP-5421-250g

SDS


Formula : NaC12

H25

SO4


IUPAC ID : Sodium dodecyl sulfate

Melting Point : 206 °C


SDS

SDS

SDS

100g

200g

500g

3460.00

6430.00

12750.00

OP-5422-100g

OP-5422-200g

OP-5422-500g

150

BIOC

HEM

ICAL

SP

RO

DU

CT

CAT

ALO

GU

E


Sodium acetate

Sodium acetate

Sodium acetate

100g

200g

500g

1040.00

1700.00

4340.00

OP-5423-100g

OP-5423-200g

OP-5423-500g

Sodium acetate




IUPAC ID : Sodium acetate

Formula : C2H

3NaO

2

Boiling Point : 881.4 °C

Soluble in : Water


TEMED - Tetramethylethylenediamine



1ml

5ml

25ml

320.00

1040.00

2415.00

OP-5424-1ml

OP-5424-5ml

OP-5424-25ml


Formula : C6H

16N

2

Density : 775.00 kg/m³



Tris-Buffer

Tris-Buffer

Tris-Buffer

100g

500g

1kg

1480.00

4840.00

8690.00

OP-5426-100g

OP-5426-500g

OP-5426-1kg

Tris-Buffer


Formula : C4H

11NO

3

IUPAC ID : 2-Amino-2-hydroxymethyl-propane-1,3-diol

Boiling Point : 219 °C


Soluble in : Water


Tris Hcl

Tris Hcl

Tris Hcl

100g

250g

500g

3650.00

8780.00

15950.00

OP-5425-100g

OP-5425-250g

OP-5425-500g

Tris Hcl

Chemical Names : Tris(hydroxymethyl)aminomethane hydrochloride; TRIS HCl; 2-Amino-

2-(hydroxymethyl)propane-1,3-diol hydrochloride


12ClNO

3

Molecular Weight : 157.59598 g/mol

151

BIOC

HEM

ICAL

SP

RO

DU

CT

CAT

ALO

GU

E


Triton -X-100

Triton -X-100

Triton -X-100

100ml

200ml

500ml

995.00

1570.00

4490.00

OP-5427-100ml

OP-5427-200ml

OP-5427-500ml

Triton -X-100

IUPAC ID : 4-(1,1,3,3-Tetramethylbutyl)phenyl-polyethylene glycol, t-Octylphenoxypolyethoxyethanol\ Polyethylene glycol tert-octylphenyl ether

Linear Formula : t-Oct-C6H4-(OCH2CH2)xOH, x= 9-10



X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside)



50mg

100mg

500mg

760.00

1150.00

9340.00

OP-5428-50mg

OP-5428-100mg

OP-5428-500mg


Formula : C14

H15

BrClNO6

Molar Mass : 408.629

152

STRA

INS

PR

OD

UC

T C

ATA

LOG

UE

STRAINSCat.No Bacterial Strains Volume Price (`)

Bacillus spp

E.coli - DH5α

E.coli -BL 21

E.coli -KL16

E.coli -PBR 322

E.coli -pUC 19

E.coli -pUC18

Lactobacillus casei

Lactobacillus plantarum

Micrococcus luteus

Mycobacterium smegmatis

Phage P1

Proteus mirabilis

Proteus vulgaris

Pseudomonas aeruginosa

Salmonella typhi

Staphylococcus aureus

1Vial

1Vial

1Vial

1Vial

1Vial

1Vial

1Vial

1Vial

1Vial

1Vial

1Vial

1Vial

1Vial

1Vial

1Vial

1Vial

1Vial

1500.00

1500.00

1500.00

1500.00

1500.00

1500.00

1500.00

1500.00

1500.00

1500.00

1500.00

1500.00

1500.00

1500.00

1500.00

1500.00

1500.00

OP-5501-VL

OP-5502-VL

OP-5503-VL

OP-5504-VL

OP-5505-VL

OP-5506-VL

OP-5507-VL

OP-5508-VL

OP-5509-VL

OP-5510-VL

OP-5511-VL

OP-5512-VL

OP-5513-VL

OP-5514-VL

OP-5515-VL

OP-5516-VL

OP-5517-VL

Cat.No Fungal Strains Volume Price (`)

Aspergillus niger

Aspergillus �avus

Aspergillus fumigatus

Candida albicans

Mucor

Rhizopus

Penicillium

1Vial

1Vial

1Vial

1Vial

1Vial

1Vial

1Vial

1250.00

1250.00

1250.00

1250.00

1250.00

1250.00

1250.00

OP-5601-VL

OP-5602-VL

OP-5603-VL

OP-5604-VL

OP-5605-VL

OP-5606-VL

OP-5607-VL

Cat.No Immunocomponents Volume Price (`)

Anti rabbit IgG

Anti rabbit HRP conjugate

Bovine IgG

Fetal Bovine Serum (FBS)

Precoated Strips (ELISA)

Rabbit IgG

Coomb’s Reagent

V.0.R*

V.0.R*

V.0.R*

V.0.R*

V.0.R*

V.0.R*

V.0.R*

P.O.R*

P.O.R*

P.O.R*

P.O.R*

P.O.R*

P.O.R*

P.O.R*

OP-5701

OP-5702

OP-5703

OP-5704

OP-5705

OP-5706

OP-5707

IMMUNOCOMPONENTS

*Price on Request*Volume on Request

153

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LAB WARES

Cat.No Lab Wares Quantity/Pack Price (`)

Chromatography Column - 3ml

Chromatography Column - 5ml

Micropipette Stand

Staining Tray

Test tube Rack - 15ml ( 31 Places )

Test tube Rack - 50ml ( 12 Places )

Test tube Rack Boiling Tubes ( 18 Places )

UV shield

25Nos

50Nos

25Nos

50Nos

1No

1No

1No

1No

1No

1No

Enquire

Enquire

Enquire

Enquire

1936.00

370.00

358.00

454.00

340.00

Enquire

CC-3ml

CC-5ml

MP-01

ST-01

TTR-15ml

TTR-50ml

TTR-BT

UVS-01

154

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ECONNECTORS

Cat.No Pack Size Price (`)

10Nos

10Nos

10Nos

10Nos

10Nos

10Nos

10Nos

Size in mm

3.2

4

4.8

6.4

8

9.5

12.7

Size in inch

1/8

5/32

3/16

1/4

5/16

3/8

1/2

900.00

900.00

900.00

900.00

900.00

900.00

900.00

SC-01

SC-02

SC-03

SC-04

SC-05

SC-06

SC-07

10Nos

10Nos

10Nos

10Nos

10Nos

10Nos

10Nos

3.2

4

4.8

6.4

8

9.5

12.7

1/8

5/32

3/16

1/4

5/16

3/8

1/2

900.00

900.00

900.00

900.00

900.00

900.00

900.00

EC-01

EC-02

EC-03

EC-04

EC-05

EC-06

EC-07

10Nos

10Nos

10Nos

10Nos

10Nos

10Nos

10Nos

3.2

4

4.8

6.4

8

9.5

12.7

1/8

5/32

3/16

1/4

5/16

3/8

1/2

900.00

900.00

900.00

900.00

900.00

900.00

900.00

TC-01

TC-02

TC-03

TC-04

TC-05

TC-06

TC-07

10Nos

10Nos

10Nos

10Nos

10Nos

10Nos

10Nos

3.2

4

4.8

6.4

8

9.5

12.7

1/8

5/32

3/16

1/4

5/16

3/8

1/2

1350.00

1350.00

1350.00

1350.00

1350.00

1350.00

1350.00

YC-01

YC-02

YC-03

YC-04

YC-05

YC-06

YC-07

STRAIGHT CONNECTOR

ELBOW CONNECTOR

T SHAPE CONNECTOR

Y SHAPE CONNECTOR

155

ORANGE proudly introducing a quality and skill based Industrial training program and projects for students.

Salient features• Students will gain 100% exposure in handling all instruments.• Both wet and dry lab skills development.• Each module includes basic theoretical and practical classes.• Students can learn application followed by self evaluation of techniques. • Calculations relevant to molecular biology, chemical preparation, hands on practice, observation, interpretation and justi�cation of the results.

Who Need?People working in the �eld of,• Research & Development• Pharmaceutical industry• Immunology• Molecular biology• Forensics• Biotechnology• Recombinant technology• Microbiology

Modules1. Basic, Mid level & Advanced level 1 and 2 in Molecular biology2. DNA Fingerprinting 3. Variant PCR Reactions4. Transcriptomics5. Proteomics6. Basic Genetics7. Blotting techniques8. Chromatography techniques 9. DNA modi�cation system10. DNA mutation 11. Immunodiffusion techniques12. Hemagglutination13. ELISA techniques14. Microbial techniques15. Protein Isolation and puri�cation study16. Cloning17. Advanced cloning & Expression of protein 18. Enzyme Technology

Learn these modules at :ORANGE20/14, Sapthagiri colony,Jafferkhanpet, Ashok NagarChennai -600083, Tamilnadu, India.Ph : 044 - 6545 5597, 2371 3341Mail: [email protected]

ORANGE“An Institute for Industrial Skill Development”

INDUSTRIAL TRAINING PROGRAM FOR LIFE SCIENCE STUDENTS.

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SA ACE Series Maxi Horizontal Immersed Gel Electrophoresis Unit 10ACE Series Midi Horizontal Immersed Gel Electrophoresis Unit 8ACE Series Midi Super Horizontal Immersed Gel Electrophoresis Unit 9ACE Series Mini Horizontal Immersed Gel Electrophoresis Unit 6ACE Series Mini Super Horizontal Immersed Gel Electrophoresis Unit 7

C Chromatography Column 153Colony Counter 51Connectors 154

D Digital Power Supply 300V/100mA 35Digital Power Supply 300V/200mA 36Digital Power Supply 500V/500mA 37Dual Tier Gel Rocker 50

E Ease Advanced Midi SemiDry Trans Blot Apparatus 26Ease Advanced Mini SemiDry Trans Blot Apparatus 25

G Gel Rocker With Electronic Timer 49Gel Rocker Without Timer 49

H High Current Power Supply 39High Voltage Power Supply 39Horizontal Paper Gel Electrophoresis Unit 31

J Junior Horizontal Immersed Gel Electrophoresis Unit 2

M Maxi Broad Vertical Gel Electrophoresis Unit (SDS - PAGE Apparatus) 15Maxi Horizontal Immersed Gel Electrophoresis Unit 5Maxi Horizontal Immersed Gel Electrophoresis with Water Circulation Unit 5Maxi Vertical Gel Electrophoresis Unit (SDS - PAGE Apparatus) 14Micropipette Stand 153Midi 2D PAGE Gel Unit 33Midi Dual Vertical Gel Electrophoresis Unit (SDS - PAGE Apparatus) 13Midi Horizontal Immersed Gel Electrophoresis Unit 4Midi Immuno Gel Electrophoresis Unit 29Midi Southern Blot Apparatus 27Midi Tube Gel Unit 32Midi Vertical Gel Electrophoresis Unit (SDS - PAGE Apparatus) 13Midi Vertical Paper Gel Electrophoresis Unit 30Mini 2D PAGE Gel Unit 32Mini Dual Vertical Gel Electrophoresis Unit (SDS - PAGE Apparatus) 12Mini Horizontal Immersed Gel Electrophoresis Unit 3Mini Immuno Gel Electrophoresis Unit 28Mini Southern Blot Apparatus 27Mini Tube Gel Unit 31Mini Vertical Gel Electrophoresis Unit (SDS - PAGE Apparatus) 11Mini Vertical Paper Gel Electrophoresis Unit 30

P PCR Work Hood 47Power Supply 250V/1000mA 38Power Supply Basic 37Power Supply Basic Dual 38Programmable Power Supply 300V/100mA 34Programmable Power Supply 300V/200mA 34Programmable Power Supply 500V/500mA 35Programmable Power Supply 300V/400mA 36Protwins Midi Dual Wet Blot Apparatus 20Protwins Midi Trio Wet Blot Apparatus 20Protwins Midi Wet Blot Apparatus 19Protwins Mini Dual Wet Blot Apparatus 18Protwins Mini Trio Wet Blot Apparatus 19Protwins Mini Wet Blot Apparatus 18

R Rapid Midi Dual Wet Blot Apparatus 23Rapid Midi Trio Wet Blot Apparatus 23Rapid Midi Wet Blot Apparatus 22Rapid Mini Dual Wet Blot Apparatus 21Rapid Mini Trio Wet Blot Apparatus 22Rapid Mini Wet Blot Apparatus 21Regular Midi SemiDry Trans Blot Apparatus 25Regular Mini SemiDry Trans Blot Apparatus 24

S Staining Tray 153

T Table Top pH Meter 50Test tube Rack 153TLC Viewer 48

U UV shield 153UV Transilluminator BLB 41UV Transilluminator BLB Basic 41UV Transilluminator BLB Research 41UV Transilluminator Vista 42UV TRIO Transilluminator 44UV Ultra Dual Transilluminator 44UV Ultra Transilluminator 43UV VIVID Transilluminator 43UV-BI Wavelength Transilluminator 43UVFL Portable Lamp With UV Glass Filter 46UVHL Portable Lamp 46UVL Portable Lamp 45UVM Portable Lamp 45UVS Portable Lamp 45UVS Ultra Portable Lamp 46UV-VIZ Basic Transilluminator 42UV-VIZ Ultra Transilluminator 42

W White Illuminator 44

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Genomic DNA Extraction from Plant using CTAB Method Teaching Kit 70Genomic DNA Extraction from Whole Blood Teaching Kit 71Genomic DNA Extraction from Yeast Teaching Kit 71Genomic DNA From Bacillus subtilis 119Genomic DNA From Escherichia coli 119Genomic DNA from Human Blood 119Genomic DNA from Plant 119Genomic DNA from Pseudomonas aeuroginosa 119Genomic DNA from Yeast 119Genomic DNA Pur�cation By Using DNA Binding Membrane Teaching Kit 71GFP Cloning Teaching Kit 71Giemsa Stain 136Glycerol (50%) 136GOD/POD Teaching Kit 104Gram Staining Teaching Kit 114GTE Buffer (50mM) 124Guanidine hydrochloride 148

H Haemagglutination Teaching Kit 97Haemoglobin (Hb) Estimation By Cyanmethaemoglobin Method Teaching Kit 104HBSS buffer 124Hind III 122Histone Isolation From Nuclei Teaching Kit 86Horse raddish Peroxidase 121

I IgG Puri�cation Teaching Kit 100IgY Puri�cation and analysis by SDS-PAGE Teaching Kit 87Immobolized Metal Ion Af�nity Chromatography Teaching Kit 100Immuno Buffer (20X) 128Immunoeletrophoresis Teaching Kit 107Immunoglobulin Puri�cation Teaching Kit 100Immunohistochemistry Teaching Kit 87Immunoprecipitation Teaching Kit 107Invitro Transcription Teaching Kit 93Iodine Solution ( Gram Stain) 136Iodine solution (0.05M) 136Iodine-Potassium iodide solution (For Starch Test) 136Ion Exchange Chromatography (Antibody Puri�cation ) Teaching Kit 100Ion Exchange Chromatography (Lysozyme Puri�cation) Teaching Kit 101IPTG 148Isoelectrofocusing and 2D gel Electrophoresis (Tube gel) Teaching Kit 87Isolation and Characterization of Petite mutant of Yeast Teaching Kit 72Isolation And Identi�cation Of Antibiotic Producing MicroorangismTeaching Kit 110Isolation and Identi�cation of DNA and RNA Teaching Kit 72Isolation And Identi�cation Of Enzyme Producing MicroorganismTeaching Kit 110Isolation And Identi�cation Of Rhizobium From Root Nodules Teaching Kit 110Isolation And Identi�cation Of Soil Bacteria Teaching Kit 111Isolation And Identi�cation Of Yeast From Curd Or Fruit Juice Teaching Kit 111Isolation of Antibiotic Resistant and Auxotrophic Mutant of yeastTeaching Kit 72Isolation of Lymphocytes Teaching Kit 105Isolation Of Molds From Soil Teaching Kit 111Isolation of Monocytes Teaching Kit 105Isolation of Rosette Cells Teaching kit 105

K Kovac's Reagent 137

L Lactobacillus casei 152Lactobacillus plantarum 152Lactophenol Cotton Blue Staining Teaching Kit 114Lambda DNA 119Lambda DNA + ECORI Marker 120Lambda Hind III + ECORI Marker 120Lambda Hind III Marker 120Lambda Phage DNA Isolation Teaching Kit 73Latex Agglutination Teaching Kit 97Ligation Teaching Kit 73Long PCR Teaching Kit 73Lysozyme 121

M Malachite Green Solution 137Mammalian Protein Extraction Kit 60

Metachromatic Granules Staining Teaching Kit 114MgCl2 (25mM) for PCR 143Micrococcus luteus 152Mitochondrial DNA Isolation Teaching Kit 74M-MuLV Reverse Transcriptase 143Monochrome Staining Teaching Kit 115MOPS Buffer (10X) 128mRNA Puri�cation Kit 57MTT Assay Teaching Kit 117Mucor 152Multiplex PCR Teaching Kit 74Mutation Detection and Analysis (Mutagenesis) Teaching Kit 74Mycobacterium smegmatis 152

N NATIVE -PAGE Teaching Kit 88Negative Staining Teaching Kit 115Nested PCR Teaching Kit 75Neutral Red Solution 137Normal Saline 137Northern Blotting Teaching Kit 93Northern Hybridization Teaching Kit 93NP-40 149Nucleosome Mapping by Micrococcal Nulease (Mnase) DigestionTeaching Kit 75

O Oligonucleotide Puri�cation Teaching Kit 75Optimization of time of Inducer Concentration on E.coli Recombinant Protein Expression 76Orange Gel Loading Dye (6X) 137Ouchterlony Double Immuno Diffusion (Pattern) Teaching Kit 108Ouchterlony Double Immuno Diffusion (Titration) Teaching Kit 108

P Paper Chromatography Teaching Kit 101pBR 322 119PCR Ampli�cation of β-Galactosidase Gene Teaching Kit 76PCR Application Teaching Kit 76PCR Buffer 143PCR Cleanup Extraction Kit 58PCR Master Mix with Taq Polymerase 143PCR Products Puri�cation Kit 58PCR Teaching Kit 76PCR water 143Penicillium 152pH Standard 10.0 137pH Standard 4.0 138pH Standard 7.0 138Phage P1 152Phage Titration Teaching Kit 77Phenol 149Phenol : Chloroform : Isoamylalcohol (25:24:1) 138Phenol Tris Saturated 138Phenol water Saturated 138Phenol:Chloroform 139Phosphate Buffer Saline (PBS)10X 125Phosphate buffer saline Tween-20 (PBST)(10X) 125Plant DNA Extraction Kit (For PCR Ampli�cation) 60Plant RNA Isolation Teaching Kit 94Plant Tissue Culture Teaching Kit 116Plasmid Curing Teaching Kit 77Plasmid DNA Isolation Teaching Kit 77Plasmid Puri�cation by DNA Binding Membrane Teaching Kit 78Polyvinylpyrrolidone (PVP) 149Ponceau's Stain (2X) 139Precoated Strips (ELISA) 152Pregnancy Test (Card Method)Teaching Kit 105Pregnancy Test (Slide Method)Teaching Kit 106Preparation of Biotinylated Probes by Nick Translation Teaching Kit 88Preparation Of Chicken Embryo Fibroblast (In CO2 Incubator) Teaching Kit 116Preparation of Poly(A) mRNA From Yeast Teaching Kit 94Protein Agarose Gel Electrophoresis Teaching Kit 88Protein Degradation Teaching Kit 88Protein Estimation Teaching Kit 89Protein Folding Teaching Kit 89Protein marker-Broad Range 120Protein marker-High range 120

A ABO Blood Grouping With Rh-Factor Teaching Kit 96Acid Fast Staining Teaching Kit 113ACK lysis Buffer 124Acrylamide 145Acrylamide / Bis- Acrylamide -30% (28 : 2) 131Acrylamide / Bis- Acrylamide -30% (29 : 1) 131Acrylamide / Bis- Acrylamide -35% (34 : 1) 131Af�nity Chromatography Teaching Kit 99Agarose Gel Electrophoresis Teaching Kit 64Agarose High EEO 145Agarose Low EEO 145Alkaline Phosphatase 121Alu I 122AMES Teaching Kit 64Amidoblock Stain 131Ampicillin(Sodium salt) 145Analysis of blood components Teaching Kit 103Analysis of Protein Structure Teaching Kit 84Andrade's Indicator 131Anti rabbit HRP conjugate 152Anti rabbit IgG 152Antibiotic Sensitivity Test Teaching Kit 111Antibody Biotin Conjugation Teaching Kit 84Antibody Capture ELISA Teaching Kit 101Antibody FITC Conjugation Teaching Kit 84Antibody HRP Conjugation Teaching Kit 85Antigen Capture ELISA Teaching Kit 102ASO Latex Agglutination Teaching Kit 96Aspergillus �avus 152Aspergillus fumigatus 152Aspergillus niger 152Assay buffer (10X) 124

B Bacillus spp 152Bacterial Alkaline Phosphatase Activity Teaching Kit 85Bacterial Cell Lysis Reagent Kit 59Bacterial Conjugation Teaching Kit 64Bacterial Gene Expression Teaching Kit 65Bacterial Genomic DNA Extraction Kit (Quick one Step Method) 59Bacterial Growth Curve Teaching Kit 112Bacterial Protein Extraction Kit 59Baker's Softening Fluid (Softening of Animal Tissue) 132Bam H I 122Barfoeds reagent 132Benedicts Qualitative Solution 132Beta Mercaptoethanol (BME) 146Bisacrylamide 145Blotting Buffer 128Bovine IgG 152Bovine Serum Albumin (BSA) 146Bovine Serum Albumin solution (10%) 132Bradfords reagent 132Bromophenol Blue (BPB) 146

C Candida albicans 152Capsular Staining Teaching Kit 113CBB-G250 Stainer (4X) 133CBB-R250 Stainer (4X) 133Cellulose Acetate Electrophoresis Teaching Kit 85Cetyltrimethyl Ammonium Bromide (C-TAB) 147Chloroplast DNA Isolation Teaching Kit 65Chromosomal DNA Extraction from Bacteria Teaching Kit 65Clone Gene Analysis by SDS PAGE Teaching Kit 86Colloidal Protein staining solution 133Column Chromotography Teaching Kit 99Competent Cell Preparation Teaching Kit 65Competitive ELISA Teaching Kit 102Complement Fixation Test Teaching Kit 96Coomassie Brilliant Blue G-250 (CBB-G250) 146Coomassie Brilliant Blue R-250 (CBB-R250) 147Coomb's reagent 152Counter Current Immunoelectrophoresis Teaching Kit 107C-Reactive Protein (CRP) Latex Teaching Kit 97Crystal violet (Gram Stain) solution 133Crystal Violet solution 133

Culture Of HeLa S3 Cells In Suspension ( without CO2 System)Teaching Kit 116Cyanomethemoglobin (20mg /ml) 134

D dATP Solution 143dCTP Solution 143Denaturing Gel Electrophoresis for DNA Sequencing Teaching Kit 66Denhardt's solution (50X) 134Destainer 134Determination of Tm Value Teaching Kit 66Determination of DNA by Colorimetric method Teaching Kit 66dGTP Solution 143Diethylpyrocarbonate (DEPC) 147Dimethyl Sulphoxide (DMSO 147Diphenylamine Reagent 134DNA Ladder (100bp) 120DNA Estimation Teaching Kit 66DNA Estimation by DPA Method Teaching Kit 67DNA Fingerprinting Teaching Kit (Using AFLP Technique) 67DNA Fingerprinting Teaching Kit (Using RAPD Technique) 67DNA Fingerprinting Teaching Kit (Using RFLP Technique) 67DNA Ladder (1kb) 120DNA Molecular Weight Determination Teaching Kit 68DNase I 121dNTP Mix Solution 143dNTP Mix Solution 143Dot ELISA Teaching Kit 102Drabkin's Reagent 134DTT -Dithiothreitol solution (1M) 134dTTP Solution 143

E E.coli - DH5α 152E.coli -BL 21 152E.coli -KL16 152E.coli -PBR 322 152E.coli -pUC 19 152E.coli -pUC18 152ECOR I 122ECOR V 122EDTA -0.5M 135Effect Of Different pH On Bacterial Growth Teaching Kit 112Effect Of Different Salt Concentration On Bacterial Growth Teaching Kit 112Effect Of Different Temperature On Bacterial Growth Teaching Kit 112Endospore Staining Teaching Kit 113Enumeration Of RBC with Hemocytometer Teaching Kit 103Enumeration Of RBC without Hemocytometer Teaching Kit 103Enumeration Of WBC with Hemocytometer Teaching Kit 104Enumeration Of WBC without Hemocytometer Teaching Kit 104Enzyme Isolation from Bacteria Teaching Kit 86Enzyme Kinetics Teaching Kit 86Eosin Stain 135Estimation of RNA by Orcinol Method Teaching Kit 93Ethidium Bromide 148Ethidium Bromide (10mg / ml) 135Ethylenediaminetetraacetic acid (EDTA) 148

F FACs buffer 124Fehling's A & B Solution 135Fetal Bovine Serum (FBS) 152Flagella Staining Teaching Kit 114Folin's reagent 135Formamide 148

G Gel Extraction Teaching Kit 68Gel Filtration Chromatography Teaching Kit 99Gel Loading Dye (6X) 135Gene Cloning Teaching Kit 68Genomic DNA Extraction from Animal Tissue (High Salt Method)Teaching Kit 69Genomic DNA Extraction from Animal Tissue Teaching Kit 69Genomic DNA Extraction from Bacteria Teaching Kit 69Genomic DNA Extraction from Fungi Teaching Kit 70Genomic DNA Extraction from Plant Teaching Kit 70

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Protein marker-Low Range 120Protein marker-Medium Range 120Protein Standard (BSA 1mg/ml) 139Proteinase K 121Proteus mirabilis 152Proteus vulgaris 152Protoplast Fusion Teaching Kit 78Pseudomonas aeruginosa 152Pst I 122pUC 18 DNA 119PUC 19 DNA 119

Q Quantitative Preciptin Assay Teaching Kit 108

R RA (Rheumatoid Arthritis) Test Teaching Kit 97Rabbit IgG 152Rapid Plasmid Screening Teaching Kit 78RBC Diluting Fluid 139RBC lysis Buffer 125Reporter Gene Assay Teaching Kit 79Restriction Analysis of Plasmid (pBR322 & pUC 18) Teaching Kit 79Restriction Digestion Teaching Kit 79Restriction Mapping Teaching Kit 80Rhizopus 152Ringer's Solution 139RIPA buffer 125RNA later Solution 140RNase A 121RNase Inhibitor 121Rocket Immunoelectrophoresis Teaching Kit 109RPR Test Teaching kit 98RT-PCR Teaching Kit 80

S Sal I 122Salmonella typhi 152Sample Solubilizing Buffer for NATIVE -PAGE (5X) 140Sample Solubilizing Buffer for SDS -PAGE (5X) 140Sandwich ELISA Teaching Kit 102SDS 149SDS- PAGE Teaching Kit 89SDS Solution 10% 140Seliwanoffs reagent 140Separating gel mix 140Serum Protein Electrophoresis Teaching Kit 90SGOT Teaching Kit 106SGPT Teaching Kit 106Silver Staining Teaching Kit 90Simple Staining Method For Bacteria In Milk Teaching Kit 115Simple Staining Teaching Kit 116Single Nucleotide Polymorphisms (SNP) Teaching Kit 80Single Radial Immunodiffusion Teaching Kit 109Single Step Ultra-competetent cell preparation Kit 60Site Directed Mutagenesis by PCR Teaching Kit 80SM Buffer 125Sma I 122Sodium acetate 150Sodium Acetate (5M) 141Sodium Citrate Buffer 126Southern Blotting Teaching Kit 81Southern Hybridization Teaching Kit 81SSC buffer 126SSPE Buffer (20X) 128Stacking Gel Mix (4%) 141Staphylococcus aureus 152Starch Standard(10mg/ml) 141STET Lysis Buffer 126Study Of Apoptosis By Staining Method Teaching Kit 117Study of Calf Intestine Alkaline Phosphatase Activity (Dephosporylation of DNA)Teaching Kit 90Study of Cellular Fractionation Teaching Kit 91Study of DNA Methylation Teaching Kit 81Study of DNA Repair Teaching Kit 82Study of Phyical and Chemical Mutagens on The Survival of E.coli Growth Teaching Kit 82Study of Physical Properties of Protein Teaching Kit 91Study of Protein Fingerprinting Teaching Kit 91

T T4 DNA Ligase 121TAE(10X ) 129TAE(50X ) 129Taq Polymerase 143TE Buffer (10X) 126TEB(10X) 129TEMED (Tetramethylethylenediamine) 150Thin Layer Chromotography Teaching Kit 101Total RNA extraction Kit 59Total RNA Extraction Kit ( By Trizol) 60Total RNA Extraction and RT-PCR Teaching Kit 94Total RNA Extraction from Bacteria Teaching Kit 95Total RNA Extraction From Yeast by Trizol Method Teaching Kit 95Total RNA Extraction From Yeast Teaching Kit 95Transduction Teaching Kit 82Transformation (Blue-White Screening ) Teaching Kit 82Transformation of Yeast Cell Using Lithium Acetate Teaching Kit 83Tris - Glycine Buffer for NATIVE -PAGE (10X) 129Tris - Glycine -SDS Buffer for SDS-PAGE (10X) 129Tris Buffer 150Tris Buffer pH 6.8 127Tris Buffer pH 8.8 127Tris buffer saline (TBS)(10X) 127Tris buffer saline Tween-20 (TBST)(10X) 127Tris Hcl 150Tris-tricine Buffer (10X) 129Triton-X-100 151Trizol 141Trypan Blue solution 0.4% 141

U Ultrapure DNA Extraction from Soil sample 54Ultrapure DNA Gel Extraction Kit 54Ultrapure Genomic DNA Extraction from Bacteria 54Ultrapure Genomic DNA Extraction from Blood 55Ultrapure Genomic DNA Extraction from Bone 55Ultrapure Genomic DNA Extraction from Frozen Tissue 55Ultrapure Genomic DNA Extraction from Hair 56Ultrapure Genomic DNA Extraction from Nail 56Ultrapure Genomic DNA Extraction from Plant 56Ultrapure Genomic DNA Extraction from Plant (CTAB Method) 57Ultrapure Genomic DNA Extraction from Tissue 57Ultrapure Plasmid DNA Extraction Kit 58

V VDRL Test Teaching Kit 98VP Reagent 142

W Water (DEPC Treated) 142WBC Diluting Fluid 142WBC lysis Buffer 127Western Blotting Teaching Kit 92Whole blood DNA lysis buffer 128Whole Blood Genomic DNA Extraction Kit (From Fresh and Frozen Blood) 60Widal Slide Test Teaching Kit 98Widal Tube Test Teaching Kit 98

X Xba I 122X-Gal 151

Y Yeast Plasmid DNA Extraction Teaching Kit 83

160

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ONS

Terms & Conditions

• All the prices are in INR.

• The prices mentioned in catalog are subject to F.O.R Station.

• CST / VAT Insurance will be charged extra as applicable.

• The goods will be supplied through surface cargo or air cargo based on customers need.

• We will try to dispatch goods from our ready stock otherwise delivery schedule will be in a week.

• We ensure you to provide healthy packing but our responsibility ceases once the goods have dispatched our facility.

• We reserve right to modify colour/design/speci�cation any time for advancement of our products.

• Pictures shown in this catalogue are modi�ed for best printing. Actual product may vary from the pictures shown in this catalogue.

• This catalogue includes ORANGE brand product and also associated brands of ORANGE modules.

• All disputes are subject to Chennai jurisdiction only.

• To avoid misconception please specify Page No., Catalogue No., Product description, Pack, Quantity, Mode of Dispatch in Purchase Order.

• We require minimum 3-5 working days to process order from ready stock.

• Please contact ORANGE local dealer from your region or distributor for more information.

161



1 30000.00DPS6003

Speci�cation:

NEW ARRIVALS

Make : ORANGE


Cat.No : DPS6003







Display : LCD







162


1 17550.00VDA0001

Speci�cation:

Make : ORANGE

Model : Advanced- Verical Dual

Cat.No : VDA0001

Number of Samples : 20 (2 Gel)


Gel Size : 10cm x 10cm(WxL) (2 Gel)


Number of Combs : 10-wells 1mm thickness comb (2 Nos.)

10-wells 1.5mm thickness comb (2 Nos.)


Gasket : 4 Nos.

Comb Capacity : 35μl

Glass Holder and Screws : 1 Set



Lower Buffer Chamber : 1 No.

Safety Lid : 1 No.

Electrode Unit With Water Circulation Port : 1 No.

Spacer Placer : 1 No.

Blocking Plate : 1 No.

Gel Casting Pouch : 20 Nos.

Gel Caster : 1 No.

Surface Equalizer : By Bubble Level



Advanced Vertical Dual Gel ElectrophoresisWith Water Refresher System ( SDS - PAGE Apparatus)

Advanced Vertical Dual Gel Electrophoresis With Water Refresher System ( SDS - PAGE Apparatus), which comes

with unique design and simpli�es the casting process. This is a newly emerging advanced vertical dual gel electropho-

resis with water refresher which allows the gel to run for longer time without any damage to gel or sample to be ana-

lysed.

161



1 30000.00DPS6003

Speci�cation:

NEW ARRIVALS

Make : ORANGE


Cat.No : DPS6003







Display : LCD







162


1 17550.00VDA0001

Speci�cation:

Make : ORANGE

Model : Advanced- Verical Dual

Cat.No : VDA0001

Number of Samples : 20 (2 Gel)


Gel Size : 10cm x 10cm(WxL) (2 Gel)


Number of Combs : 10-wells 1mm thickness comb (2 Nos.)

10-wells 1.5mm thickness comb (2 Nos.)


Gasket : 4 Nos.

Comb Capacity : 35μl

Glass Holder and Screws : 1 Set



Lower Buffer Chamber : 1 No.

Safety Lid : 1 No.

Electrode Unit With Water Circulation Port : 1 No.

Spacer Placer : 1 No.

Blocking Plate : 1 No.

Gel Casting Pouch : 20 Nos.

Gel Caster : 1 No.

Surface Equalizer : By Bubble Level



Advanced Vertical Dual Gel ElectrophoresisWith Water Refresher System ( SDS - PAGE Apparatus)

Advanced Vertical Dual Gel Electrophoresis With Water Refresher System ( SDS - PAGE Apparatus), which comes

with unique design and simpli�es the casting process. This is a newly emerging advanced vertical dual gel electropho-

resis with water refresher which allows the gel to run for longer time without any damage to gel or sample to be ana-

lysed.

• Easy and Safety Handling (Away from Acrylamide Hazardous to avoid Cancer).

• Casting slab gels with this system combines a clever design and a simple technique.

• Preparation of Maximum Size Gel by utilizing the lower spacer's space.

• Easy arrangements of Spacers with the help of Gel Casting Pouch.

• Preparation of equal size Gel with the help of Bubble Leveller which avoid immproper Run.

• Easy placement of Gel in the Unit with casual screws and clamps.

• Low consumption of Buffer ( Apprx. 250ml) .

• The central cooling core is composed of thermally conductive plates and a unique �uid circulation path.

• Water Circulation Port (Maintain Temperature) helps to Run the gel without any damage.

• 20 Samples can be analyzed in a single Run.

Advanced Vertical Dual Gel Electrophoresis With WaterRefresher System is user friendly because

• Easy and Safety Handling (Away from Acrylamide Hazardous to avoid Cancer).

• Casting slab gels with this system combines a clever design and a simple technique.

• Preparation of Maximum Size Gel by utilizing the lower spacer's space.

• Easy arrangements of Spacers with the help of Gel Casting Pouch.

• Preparation of equal size Gel with the help of Bubble Leveller which avoid immproper Run.

• Easy placement of Gel in the Unit with casual screws and clamps.

• Low consumption of Buffer ( Apprx. 250ml) .

• The central cooling core is composed of thermally conductive plates and a unique �uid circulation path.

• Water Circulation Port (Maintain Temperature) helps to Run the gel without any damage.

• 20 Samples can be analyzed in a single Run.

Advanced Vertical Dual Gel Electrophoresis With WaterRefresher System is user friendly because

www.orange.org.in

No.20/14 Sapthagiri Colony, JafferkhanpetAshok Nagar, Chennai - 600 083. Tamilnadu, India.

Voice : +91 44 6545 5597, 2371 3341Email : [email protected], [email protected]

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Made in India by:

Authorized Dealer

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