Proceedings ofESBES-2 - 2nd European Symposium on

Biochemical Engineering Science

Edited bySEBASTIAO FEYO DE AZEVEDO, EUGE>JIO C FERREIRA ,

KAREL CH.A.M. LUYBEN AND PATRICIA OSSEWEIJER

A four-day symposium organised under the auspices of the European Federation of Biotechnology Section

on Biochemical Engineering Science and held at Seminario de Vilar Conference Centre, Porto, Portugal,

September 16-19,1998.

UB/TIB Hannover 89118948504

General Index

PLENARY LECTURES 1_

Biotechnology for the Improvement of the Quality of Life: Yesterday, Today, Tomorrow 3R. Braun

Ligand Engineering Using Combinatorial Protein Chemistry 6PA. Nygren and M. Uhlen

Bioprocess Scale-up Strategy based on Integration of Fluid Dynamics and MicrobialPhysiology 7S.-O. Enforsetal.

Proposal for a Council Decision: Adopting a Specific Programme for Research,Technological Development and Demonstration on "Quality of Life andManagement of Living Resources" (1998 to 2002) 13T. Ingemansson

Biochemical Production and the Environment - an Example 14Klaus Hansen

Biochemical Engineering education for the future 20John Villadsen

Industry & University: Partners or Parasites 23DrlrJ.C. van Suijdam

ORAL PRESENTATIONS: HEALTH CARE PRODUCTION PROCESSES 25^

Application of Microwave Technology to High Temperature Short Time Heat Treatmentof Biopharmaceutical Proteins 27Joachim K Walter et al.

Engineering Aspects for Production of Therapeutic Proteins by Animal Cell Culture 30J. Lehmann

Expression Strategies for Functional Genomics 32M. Schuster et al.

Prediction of Performance and Optimisation of Membrane Bioseparations - Ab InitioApproaches 37W. Richard Bowen et al.

Affinity Partition Studies with Penicillin Acylase from Escherichia Coli 45J. C. Marcos et al.

ORAL PRESENTATIONS: PRODUCTS FROM ENVIRONMENTAL PROCESSES 51

Biopolymer Production as a By Product of Wastewater Treatment Plant 53MAM. Reisetal.

Microbial Polysaccharide Products 59Ian W. Sutherland

Hydrogen Sulfide Produced from Sulfate by Biological Reduction for use inMetallurgical Operations 63Henk Dijkman et al.

Utilization of Cheese Whey for the Production of Penicillin G Acylase from Bacillusmegaterium ATCC 14945 72CO. Hokkaetal.

Bioprocess Engineering For Xylitol Production From Lignocelulosic Residues 78S.S. Silvaetal.

ORAL PRESENjWriONS^jIOPROCESSES FOR FOOD PRODUCTION M

Biotechnology: Food for Thought 8 5

J. TramperOptimization of the Production of Dextransucrase from Leuconostoc mesenteroides

NRRL B-1299 and its Application to the Synthesis of Non DigestibleGlucooligosaccharides 86Pierre Monsan et al.

Tortuosity in Bioseparations and its Application to Food Processes 93Manuel Mota et al.

Development of new enzymes for food applications by modem biotechnology 99Sven Pedersen et al.

Dependence of Morphology and Protein Production on Agitation Intensity inContinuous and Fed-batch Cultures of Aspergillus oryzae 102A. Amanullah et al.

ORAL PRESENTATIONS: HEALTHY PRODUCTS 113̂

Functional Foods: Scientific Basis and Future Requirements 115J.-R. Neeseretal.

Mammalian Cells for Reliable, High Yield and Fast Production of RecombinantProteins, from Micrograms to Kilograms 129Florian M. Wurm

Computer Simulation of the Competition Between Protein Folding and Aggregation 131Carol K. Hall et al.

Coupling of Immobilised Enzyme Reactions with Electro-transport Processes 138P. Hasal et al.

The Influence of Peptide Adsorption on Interfacial Tension 147A.P.J. Middelbergetal.

Biological Removal of Ammonia from Sludge Digestor Effluents with the CombinedSharon - Anammox Process -j53J. Gijs Kuenen et al.

Bioelimination of Xenobiotic Compounds: Rationally Designed Strain Versus AdaptedMixed Cultures 1 c 7H.-J. Knackmuss

Anaerobic Waste Water Treatment and Reactors 153Alexander Aivasidis

New Technological Trends in Lactic Acid Bacteria Fermentations 169B Moll&tB. Moll&t

Future Technology in the Malting and Brewing IndustryR.JR. Reed 07

Xylanase Production by two Strains of Recombinant Saccharomyces Cerevisiae inBatch, Fed-Batch and Continuous Cultures 183C. F. Wahlbometal.

POSTERS: HEALTH CARE PRODUCTION PROCESSES 191

Multiple-Limited and Energy Sufficient Microbial Growth 193U. von Stockar, N. Schill, I.W. Marison, Ph. Duboc

Whole Cell Biotransformation of Hydrophobic Compounds in a Closed Gas LoopBioreactor 194G.H.O. Steinig, A.G. Livingston and DC. Stuckey

Monitoring of genes that respond to process related stress in large-scale fermentations 195Thomas Schweder, Elke Kruger, Bo Xu, Britta Jurgen, Jorg Mosterz, Sven-Olof Enfors andMichael Hecker

Short Cut of Protein Purification by Integration of Cell-disrupture and Affinity Extraction 196E. Wasserbauer, M. Schuster, C. Ortner, K. Graumann, A. Jungbauer, F. Hammerschmidand G. Werner

The Measurment of the Mechanical Strength of an Actinomycete 197S.M. Stocks andC.R. Thomas

Rheology, Morphology and Growth In Submerged Fermentation with FilamentousMicroorganisms 198Y.Q. Cui, R.G.J.M. van derLans, K.Ch.A.M. Luyben

A Novel Bioreactor for Plant Cells Culture of E. ulmoides Callus for Producing a Factorfor Enhancing Collagen Synthesis of Animal Cells (FECS) 199Hajime Unno, Xin-huiXing, Akinori Ono and Yasunori Tanji

Characterization of the different extracellular lipases and esterases produced byCandida rugosa yeast in a pilot plant fermenter 200A. Sanchez, A. Serrano, C. Lopez, M. Pernas, M.L. Rua, P. Ferrer, F. Valero, C. CasasandC. Sola

Rational selection of cofactor regeneration processes 201Katie C. Thomas and John M. Woodley

Neural Network Control of Continuous Production in a Enzyme Membrane Bioreactor 202Z. Kurtanjek

The use of multi-staining flow cytometry to monitor batch, fed-batch and continuouscultivations of E. coli W3110 203C. J. Hewitt, G. Nebe-Von Caron, A. W. Nienow and C. M. McFarlane

Automated glucose feeding control based on CO2 Evolution Rate in Streptomycesvirginiae cultures 204E. Bugabo, Th. Marneffe, J-M. Coremans, F. Gossele and Ph. Dehottay

Compartment Model Approach: Prediction of local concentration gradients in largescale fermentations 205F.N. vanderSchot, P. Vrabel, R.G.J.M. van der Lans and K.Ch.A.M. Luyben

Recombinant A production of Saccharomyces cerevisiae in pulsed continuous culturesunder control of two different promotors 206Martin Jergensen, Adam Ryszczuk and Claus Emborg

Continous Enzymatic Production of Phenylacetylcarbinol in an Enzyme-Membrane-Reactor 207G. Goetz, P. Iwan, H. Bruhn, K. Mesch, M. Pohl and M.-R. Kula

Improvement of defined medium formulation for CHO cells by cholesterol and vitamins 208C. Altamirano, C. Paredes, J.J. Cairo, F. Godia

Chromatographic Purification of IgG from Plasma 209J.E. Amaral, J.M.S. Cabral and DM.F. Prazeres

On-line Mass Spectrometry for the Development of Reliable Software Sensors in theMonitoring of Bioprocesses 210B.S. Ferreira, F. van Keulen, M.M.R. da Fonseca

Simultaneous measurement of key analytes in a submerged fungal fermentation usingnear-infrared spectroscopy 211S. Vaidyanathan and B. McNeil

Verification of Local Oxygen-Deficient Zones in Aerobic Large-Scale E. coli Processes 212B. Xu, M. Jahic G. Blomsten and S.-O. Enfors

Potential-controlled chromatography with packed-bed electrodes for the separation ofbiomolecules 213M.W. Knizia, K. Vuorilehto, J. SchraderandD. Sell

Preliminary Study on Membrane Separation of a-Agarase 214G. Toussaint, L.H. Ding, M.Y. Jaffrin and M. Nonus

Selective Protein Separations Using Gas-Liquid Techniques 215C.J. Duffy, M.J.C. CrabbeandJ. Varley

Continuous Pseudo-Affinity Recovery and Purification of Penicillin Acylase fromEscherichia coli Crude Extracts: Selection of Adsorbent and HydrophobicAdsorption and Desorption Conditions 216LP. Fonseca, I.M. Patricio and J.M.S. Cabral

Mixing in a Large Aerated Bioreactor Equipped with Either Radial or a Radial-AxialCombination of Multiple Impellers 217P. Vrabel, R. G.J.M. van derLans, K. Ch.A.M. Luyben, L Boon, A. Nienow, S. Hjorth

Modification of Escherichia coli metabolic fluxes by metabolic engineering 218P. Vila, C. Paredes, J.J. Cairo, F. Godia

Hybrid Modelling of Biotechnological Processes Using Neural Networks 219P.Angelov, O. Bernard, G. Bastin, C. Stentelaire and M. Asther

Experimental and Theoretical Study of Cephalosporin C Adsorption in a Fixed BedColumn 220C. A. V. Burkert and F. Maugeri Filho

Modelling for Maximizing Productivity of Continuous Cultivations 221Frede Lei and Sten Bay Jargensen

A Rational Procedure for Scaling - up Bioreators: Use of Computational FluidDynamics (CFD) for the Determinaton of the Shear Stress Field, Used as a Criterion222J.A. Castillo, B. Symoens and V.L Halloin

A Kinetic Model to the Synthesis of Amoxicillin Using Penicillin G Acylase Immobilizedon Agarose 223L.R.B. Goncalves, R. Fernandez-Lafuente, R.L.C. Giordano, J.M. Guisan

Implementation of Data Acquisition System in a Bioreactor Utilizing ProgrammableLogic Controller 224A.J.G. Cruz, M.LG.C Araujo, R.C. Giordano, P.IF. Almeida and CO. Hokka

Effects of electrode dynamics on the estimate of volumetric oxygen mass transfercoefficients (kLa) in the cephalosporin C production process 225A.J.G. Cruz, AS. Silva, M.LG.C Araujo, R.C Giordano, CO. Hokka

Modelling Rejection of Proteins during Membrane Ultrafiltration 226W.R. Bowen, A.O. Sharif, M. Jain

Applications of Atomic Force Microscopy in the Study of Interactions of BiologicalMaterials 227W. Richard Bowen, Nidal Hilal, Robert W. Lovitt and Chris. J. Wright

Influence of Electrokinetic Parameters on Membrane Filtration of Escherichia coli WK-6 Cell Debris Suspensions 228A.C.C. Sousa, J.M.S. Cabral and M. Mateus

Optimal Selection of Multistep Protein Purification Processes using an Expert System:Selection Criteria and Validation 229Maria Elena LJenqueo, J. Cristian Salgado and Juan A. Asenjo

Modelling the Synthesis of a Recombinant Lytic p"-1,3-Glucanase b in Bacillus; use ofa Genetic Programming Algorithm and Optimization Studies. 230A. Olivera, M. Lay-Son, C Shene, B.A. Andrews andJ.A. Asenjo

Polypropylene Glycol Immobilized on Sepharose for the Purification ofChromobacterium viscosum Lipase 231M.M. Diogo, S.S. Ferreira, J.M.S. Cabral and JA. Queiroz

Mixing in Large Scale Bioreactors - A Numerical Study 232J. Revstedt and L Fuchs

Physiological characterization of Streptomyces submerged cultures by image analysisand electrical capacitance 233E. Crespel, S. Altaba, H. Vivier, M.N. Pons, E. Latrille, G. Corrieu, J.M. Engasser

Morphological characterization of filamentous growth 234A. Maazi, M.N. Pons, H. Vivier, E. Latrille, G. Corrieu, T Cosson

Comparison of One Stage and Two Stage Cultures of Stizolobium hassjoo Hairy RootsProducing L-DOPA by Medium Optimization Strategies 235Lin-Shiang Sung and Shih-Yow Huang

Molecular Modelling for the Study of Protein-Ligand Interaction and Ligand Design.Application to the Case of a Biomimetic Bifunctional Ligand for the Purification ofBovine Heart Lactate Dedydrogenase 236N. Labrou, £ Eliopoulos and Y. Clonis

Enhanced Oxygen Transfer on Yeast and Plant Cell Fermentation by Addition ofButyric Acid, as Oxygen-Vector 237Lima Costa, E., Pires, T.

Industrial Fermentation Processes Development. New Engineering Tools for BetterProcesses Faster. 238Jo§o P. Lopes, Andre A. Neves, Dora A. Pereira, Jose C. Menezes

Purification of Pharmaceutical Protein Using Thermoseparating Aqueous Two-PhaseSystem and Polymer Recycling 239Josefine Persson, Lena Nystro'm, Hans Ageland and Folke Tjerneld

Identification of Critical Process Parameters in The Capture Step of an Insulin AnalogUsing Fractional Factorial Experimental Design 240Ole Elvang Jensen and Jesper Christensen

Microwave Heat Inactivation of Virus in Biopharmaceutical Products 241J. K. Walter, H. Winter and U. StnWer

POSTERS: PRODUCTS FROM ENVIRONMENTAL PROCESSES 243^

Concentration and Fractionation of Cellulose-degrading Enzymes by Two-PhasePartitioning 245A. Brumbauer, G. Johansson and K. RSczey

Dynamic Modelling and Process Control for an Extractive Alcohol Production Plant 246Flavio L. Honorato da Silva, Eduardo C. Dechechi, Maria I. Rodrigues, Rubens Madel-Filho, Francisco Maugeri

Detoxification of Eucalyptus wood hydrolysates with ethyl acetate for xylitol production 247Cruz, J.M., Dominguez, J.M., Dominguez, H., Parajd, J.C

Use of entrapped Debaryomyces hansenii cells for xylitol production from woodhydrolysates 248Dominguez, J.M., Cruz, J.M., Roca, E, Dominguez, H., Parajd, J.C.

Continuous ethanol fermentation of lactose by a recombinant flocculatingSaccharomyces cerevisiae strain 249Lucilia Domingues, Maria M. Dantas, Nelson Lima and Jose A. Teixeira

Energetics of K. fragilis Growth 250L Krzystek and S. Ledakowicz

Cellulase Production of Trichoderma reesei Rut C30 using Steam Pretreated Spruce 251Zs. Szengyel, G. Zacchi and A. Varga, K. Reczey

Characterization of the Extracellular Xylanolytic Complex of a Thermophylic Strain ofBacillus licheniformis 252P. S&-Pereira and J.C Duarte

Application of Ion-Exchange Resins to Upstream Processing of Sugar Cane BagasseHydrolysate for its Utilization In Bioprocesses 253A.I. Napoles, S.S. Silva, I.M. Mancilha, M.G.A. Felipe, E. Acosta, P. Villa, I. Cepero, M.Gonzalez, E.Manganelly

Influence of Phenolic Compounds on the Bioprocess of Xylitol Production by Candidaguilliermondii 254P. Villa, M.G.A. Felipe, R.C.L Rodriguez, M. Vitolo, E. Luis dos Reis, S.S. Silva,A.I. Napoles; I.M. Mancilha

A Preliminary Study on the Fractionation of Cellulases by Ion-Exchange DisplacementChromatography 255S.M. Abrantes andJ.A. Queiroz

Studies on Cellulase Production by Trichoderma reesei Rut C-30 256F.C. Domingues, JA. Queiroz, J.M.S. Cabral and L.P. Fonseca

Influence of furfural on anaerobic glucose metabolism of Saccharomyces cerevisiae 257Eva Palmqvist, Jonas S. Almeida and Barbel Hahn-Hagerdal

Metabolic Re-Engineering of Saccharomyces Cerevisiae for the Conversion of Xyloseto Ethanol 258Aristos Aristidou, Mervi Toivari, Laura Ruohonen, Peter Richard, John Londesborough,Anita Teleman and Merja PenttilS

POSTERS: BIOPROCESSES FOR FOOD PRODUCTIONS 259

Membrane Cell Recycle Fermentations Kinetic Modelling 261A.M.R.B. Xavier, J.P.S.G.Crespo, J.A.P. Coutinho andM.J.T. Carrondo

Effect of Protein Burden on Saccharomyces cerevisiae Strains Overexpressing XyloseMetabolising or Glycolytic Genes 262N. Q. Meinander and B. Hahn-Hagerdal

Kinetic and Thermodynamic Comparison of Integrated and Sequential Reaction andSeparation Processes 263Ana L. Paiva, Diman van Rossum and F. Xavier Malcata

Comparison of Final Recovery of Product Using Integrated vs. Sequential Reactionand Separation 264Ana L Paiva and F. Xavier Malcata

Novel Polymer-Polymer Conjugates for the Recovery of Carboxylic Acids by Extractionin Aqueous Two-Phase Systems 265Jordi Planas, Antoni Kozlowski, J. Milton Harris, Folke Tjerneld and Barbel Hahn-Hagerdal

Development of a Continuous Process for Enzymatic Mannitol Production 266Matthias Slatner, Teresa Oliveira, Gudrun Nagl, Dietmar Haltrich, Bernd Nidetzky and KlausD. Kulbe

Defined Media Optimization for Production of Recombinant Mannitol-Dehydrogenasein Escherichia coli RM82 267Gudrun Nagl, Rita Quadrado, Matthias Slatner, Dietmar Haltrich, Bernd Nidetzky and KlausD. Kulbe

The Influence of Oxygen on Growth and Enzyme Production in ContinuousCultivations of Aspergillus oryzae 268Teresa Cristina Zangirolami, Jens Nielsen and Sten Bay Jergensen

The influence of biomass on the fluidization and bed stability in Expanded BedAdsorption of proteins 269H.M. Fernandez-Lahore, M.^R. Kula andJ. Thommes

Neural Network Based Modelling for Predictive Control of Bioprocesses 270A. Meszaros and A. Rusnak

Segregated Modeling of Oscillatory Saccharomyces cerevisiae Cultures 271C. Cannizzaro, I. Marison, U. von Stockar

Flexible control of microbiaf growth processes 272Kariheinz Preuss and Marie-Veronique Le Lann

Advantages of Hybrid Networks for Bioprocess Hybrid Modelling 273R. Oliveira, S. Feyo de Azevedo, R. Simutis, A. Lubbert

Structural Stability of Cutinase Encapsulated in Reversed Micelles 274AMD. Gongalves , C.M.L Caryalho, MR. Aires-Barros and J.M.S. Cabral

An Integrated Model for Enzymatic Reactions in Reversed Micellar systems: nominaland effective substrate concentrations 275C.M.L. Carvalho, MR. Aires-Barros and J.M.S. Cabral

A Membrane Bioreactor for Short Chain Esters Synthesis 276C.M.L. Carvalho, MR. Aires-Barros and J.M.S. Cabral

Metabolic flux at the pyruvate branch} point jn Saccharomyces cerevisiae: a criticalfactor for traditional and modern biotechnological applications 277J. Bauer and Peter Niederberger

Olive Oil Hydrolysis during the Microbial Production of Extracellular Lipase by theYeast Candida cylindracea CBS 6330 278Ivana Sokolovska, Milan Polakovic, Vladimir Stefuca, Vladimir Bales'

Effect of Immobilization of Cynara cardunculus cells extracts on proteolytic activity 279A.L Martins, M.E. Lima-Costa

The role of energy generation variations in the regulation of Brevibacterium flavummetabolism and lysine biosynthesis 280M.Ruklisha, R.lonina

Fusarium solani pisi cutinase partitioning in aqueous two-phase systems composed ofthermopolymers 281M.T. Cunha, J.M.S. Cabral, F. Tjerneld and M.R. Aires-Barros

Elongation of glycogen chains using a recombinant amylosucrase 282G. Potockide Montalk, M. Remaud, R.M. Willemot and P. Monsan

Electronic noses for bioprocess monitoring 283C.-F. Mandenius, Th. Bachinger and H. Liden

Isolation, Purification and Characterization of Cryophilic Proteases of Marine Origin 284J.A. Asenjo, C Barria, M. Jensen, M. Salamanca and BA. Andrews

Control of Intracellular Protein Quality by Process Integration 285F. Bylund and A. Veide

Alternative Bioprocess for Xylitol Production in a Fluidized Bed Reactor 286S.S. Silva; M.G.A. Felipe; M. Vitolo

The effect of medium composition and cell morphology on crossflow filtration ofKluyveromyces marxianus var. marxianus NRRLy2415 287AA. McCarthy, P.K. Walsh andG. Foley

Integrated fed-batch strategy dealing with substrate limitation and product inhibition inmicrobial transglutaminase production by Streptoverticillium mobaraense 288Y. Zhu, A. Rinzema, J. Tramper, E. C. de Bruin and J. Bol

Determination of the Diffusion Coefficients of Glucose and Oxygen in Floes ofSaccharomyces cerevisiae 289AA. Vicente, M. Dluhy, E.C Ferreira, M. Mota and J.A. Teixeira

Bioprocess Development and Optimisation Using Elementary Modes Analysis 290Luis C. Duarte, J.H. Woods, DA. Fell, Francisco M. Girio

Study of Oxygen Effects on Thermus sp. RQ-1 291M. Mota, I. Belo, R. Pinheiro

An Integrated Approach For Cost Effective Biosurfactant Production 292D. A. Davis, H. C. Lynch & J. Variey

Scale-up of bubble column bioreactors 293V. Bales, S". Godo

Behaviour of Debaryomyces hansenii in a continuous high dense cell culturesproducing xylitol 294

j K. Melzoch, J. Votruba, J.C.Roseiro, F.M. Girio and M. Rychtera

Investigation of Dynamic Behaviour of Immobilized Enzymes by an Enzyme FlowMicrocalorimetry 295V Stefuca, E. Kohutovicova and M. Polakovic

H-oo Robust Control of Continuous Biotechnological Process 296P.G. Georgieva, M.N. Ignatova

MIMO Adaptive Linearizing Control of Fed-Batch Amino Acids SimultaneousProduction 297M. Ignatova, V. Lubenova and P. Georgieva

Simultaneous Expression of Gluco- and a-Amylase by Aspergillus niger BO-1 duringSubmerged Cultivation 298M. Beyer, Henrik Pedersen and Jens Nielsen

Modelling the morphology of Aspergillus oryzae during Batch Cultivations Using aPopulation Model 299T. Christiansen and J. Nielsen

Expression of heterologous protein in Saccharomyces cerevisiae: production of fungalcutinase 300Philippe Duboc, Raphael Gugerii and Jens Nielsen

POSTERS: HEALTHY PRODUCTS 301_

Choice of Linked NADPH Regeneration Enzyme for Cyclohexanone MonooxygenaseCatalysed Lactone Synthesis 303Matthew C Hogan and John M Woodley

Bioprocess Applications of Molecularly Imprinted Polymers 304A. Stein, J.M. Woodley and G.J. Lye

Application of Hydrophobic Interaction to Refolding of Denatured Protein 305Hideharu Yagi and Tetsusi Sumitani

Bioelectrochemical monitoring of microbial metabolic activity 306A. Lammel, J. Schrader and D. Sell

Determining Cell-Wall Material Properties of Baker's Yeast by Micromanipulation 307Smith, A.E., Zhang, Z., Thomas, C.R., Moxham, K.E. and Middelberg, A.P.J.

Role of ATP in the Control of Proteolysis of a Recombinant Protein During Transitionto Downstream Processing 308A. Rozkov, S.O. Enfors

Direct Product Sequestration: Application to Animal Cell Derived Products 309Ian A. Carmichael, Mohamed Al-Rubeai and Andrew Lyddiatt

Carboxymethylglucan Diafiltration 310V. Sitkey and V. Visacky

Effect of CO2 Concentration on the Production and Composition of the Cell-WallPolysaccharide of Porphyridium sp. 311S. Li, S. (Malis) Arad and Y. Shabtai

Studies on the Adsorption of Plasmid DNA to Anion-Exchange ChromatographicSupports 312G.N.M. Ferreira, J.M.S. Cabral, andD.M.F. Prazeres

Analysis of Endogenous Nucleases Activity in Escherichia coli Lysates During thePrimary Isolation of Plasmids for Gene Therapy Applications 313GA. Monteiro, G.N.M. Ferreira, J.M.S. Cabral, and D.M.F. Prazeres

Development of Immunoadsorbents for the Treatment of Familial AmyloidoticPolyneuropathy (FAP) type I 314S. Riedstra, J.P.M. Ferreira, and P.M.P. Costa

The Effect of Medium Composition on the Cultivation of Streptomyces natalensis 315TA. Me Cormack, M.B. O'Shea and P.M. Kieran

Characterisation of Suspension Cultures of Drosera capensis 316S. O'Shea, D.G. O'Shea, P.M. Kieran and I. Hook

Strategies to Control the Pertinent Aggregate Size in Plant Cell Suspension Culturesfor Enhancing Secondary Metabolite Production 317S. Y. Huang, S. Y. Chen and K.W. Chen

Preliminary studies of cell suspension culture of Centaurea calcitrapa in amechanically stirred bioreactor 318S. Raposo and M. E. Lima Costa

Purification of a recombinant protein (sporamin) expressed in tobacco cells andtransgenic plants by Immobilized Metal ion Affinity Chromatography (IMAC) 319Y. Boulis and A. Vijayalakshmi

Optimization of Feeding Profiles for Sterol Biosynthesis by Yeasts Saccharomycescerevisiae 320M. Rychtera*, J. Votruba, J. Cermak, K. Melzoch, J. Fiala, J. Nahlik, Z. Vedlichova, P.Nechvile, P. Patakova, CA. Kent, T. Miskiewicz and M. Al-Rubeai

POSTERS: ENVIRONMENTAL PROCESSES 321̂

On-Line Model Identification in Wastewater Biological Treatment by Using HybridShort-Cut and Adaptive Search Techniques 323Gheorghe Maria and Cristina Maria

The membrane aerated biofilm reactor as a tool for the study of heterogeneous biofilmprocesses 324E. Casey, B. Glennon and G. Hamer

Treatability characterisation of surfactant-containing effluents by respirometrictechniques 325G. Carvalho, E. Paul, J.M. Novais and H.M. Pinheiro

Microbial behaviour during degradation of a waste gas containing alternatingchlorobenzene and 1,2-dichloroethane 326Jorge, R.M.F., and Livingston, A.G.

The Diffusion Limitations of Nitrification Processes with Gel Immobilised Bacteria in aBubble Column Reactor 327R. Polomarkaki, F. Benyahia, J. Bainbridge, and S. Manian

Selecting fungal strains for biotransformation of phenolic compounds: Decoulorisationof Remazol Brilliant Blue R and Poly-R 478 328P.M. Moreira, E. Almeida-Vara and J.C. Duarte

Ethanol from Lignocelluloses 329M. Bollok, K. Stenberg, G. Zacchi and K. Reczey

Flocculation of Escherichia coli Cell Debris with Chitosan for the Improvement ofMembrane Filtration Process 330JA.L Santos, A.C.C. Sousa, M. Mateus and J.M.S. Cabral

Glycerol bioconversion to 1,3-propanediol by Clostridium butyricum in continuouscultures with cell recycle 331M. Gonzalez-Pajuelo, J.C. Andrade and I. Vasconcelos

Biosorption of Heavy Metals in Grape Stalks 332P.. Machado, C. Santos, G. Monteiro, J.N. Correia, J. Carvalho

The Acetone - Butanol- (ABE-) Fermentation Pilot Plant Project in Austria 333JR. Gapes, D. Nimcevic, K.C. Schuster, H. Swoboda, R. Thayer, andH. Baumgartner

Bioreactor Monitoring For Wineries Wastewater Treatment 334M. Lageiro, J. Matos de Sousa, A. Eusebio, A. Ferreira and J. Cardoso Duarte

Biological Degradation of Molinate 335O.C Nunes, B. Nogales, C Nunes, N. Ramos, E.R.B. Moore, D. Pieper, J.P.S.G. Crespo,and MA. Reis

Batch and Fed-batch Cultivation Mode in Yeast Biomass Enrichment Bioprocess byChromium 336M. Batic and P. Raspor

Degradation of Chloroaromatic Compounds in a GAC Biofilm Reactor: Effect ofPerturbations 337Carvalho, F., Caldeira, M., Heald, S.C., Vasconcelos, I., Bull, AT, Castro, P.M.L

Maintenance of Heavy Metal Biosorption Systems 338Teresa Tavares, Jacinta Gongalves and Claudia Torres

Automated characterization of protozoa in activated sludge 339C. Baptiste, A L Amaral, A. Nicolau, M.N. Pons, N. Lima, E.C. Ferreira, M. Mota, H. Vivier

Study of the Microbial Communities Associated to Nitrogen and Organic MatterRemoval in Biological Wastewater Treatment Systems 340AmerE., Balaguer M.D., Poch M., CristinaX.P., FiguerasJ.B., Abella C.A.

Comparison of the Efficacy of Two Biocides: Glutaraldehyde and Carbamate 341M.O. Pereira, M.J. Vieiraand LF. Melo

Elimination of Nitrate in an Inverse Fluidized-Bed Biofilm Rector 342R. Nogueira, P. Mota, J. Ribeiro and L F. Melo

Fate of Phosphorus Concentration in a SBR Designed for Nitrogen Removal 343AC. Rodrigues, A.G. Brito, LF. Melo

Kinetic Parameters and Mass Transfer Coefficients in Pseudomonas fluorescensBiofilms 344F A Lopes, M.J. Vieira, LF. Melo

Enzyme Activities and Compost Maturity 345AC. Queda, M.J. Sebastiao andL.S. Campos

POSTERS: FOOD PRODUCTION 347

Extraction of microbial polysaccharides from fermentation broths by microfiltration onceramic membranes 349C. Harscoat, M. Y. Jaffrin and J. Courtois

Inhibiton Kinetics of 2-Phenylethanol on the Growth of Saccharomyces cerevisiae 350D. Stark, T Munch, B. Sonnleitner, I.W. Marison, U. von Stockar

Monte Carlo simulation of the a-amylolysis of amylopectin potato starch 351L.M. Marchal, R.V. Ulijn, J. Zondervan, J. Bergsma, CD. de Gooijer, J. Tramper

Mass balance modelling of vanillin production in bioreactors by the fungus Pycnoporuscinnabarinus 352O. Bernard, G. Bastin, C. Stentelaire and M. Asther

Metabolic Analysis of Glutamate Production Characteristics of Corynebacteriumglutamicum 353Pierre Gourdon and Nic D. LJndley

Carbohydrate reserves in relation to growth rate in yeast 354V. Guillou, L Plourde, J. Francois and G. Goma

Citric acid production on milled maize 355M. Minarik, V. Sitkey and J.D.van der Merwe

Production of Extracellular Thermostable Glucose Isomerase in E. coli 356S. Sariyar, B. Kirdar, and A. Hortacsu

Production and Adsorption of Lipase from Geotrichum sp using Affinity andHydrophobic Chromatographic Resin 357Kamimura, E. S., Mendieta, O., Maugeri, F.

Optimization of culture conditions for growth and formation of proteolytic enzymes byBrevibacterium linens 9172 ATCC 358W. Buchinger and WA. Hampel

The Influence of Tannins Organic Acids, and Yeast Fermentation Products on theGrowth of Two Heterofermentative Lactobacilli 359P. JR. Donovan and R.W. Lovitt

Enhanced production of Pullulan in an Ultrasound-coupled Bioreactor 360Y. Shabtai, A. Bojanover, V. Faerman, J. Kost

Stoichiometry of Citric Acid Production by A. niger 361L Krzystek and S. Ledakowicz

Cell Associated Lipase from a Cheese Related Brevibacterium linens Strain 362B.F. Adamitsch and W. Hampel

Sucrose metabolism and alternansucrase production by mutants from Leuconostocmesenteroides NRRL B-1355 363Martha A. Arguello Morales, Magali Remaud, R.M. Willemot and Pierre Monsan

Residence Time Distribution Analysis of reactors packed with Calcium alginate gelbeads containing Leuconostoc mesenteroides NRRL B-1299 insolubledextransucrase 364Marguerite Do/s, Magali Remaud-Simeon, Rene Marc Willemot and Pierre F. Monsan

Esterification by Lipase 365Belefi-Bakd, K, Csanyi, £ , Galan Serrano, MA.

Isolation, purification and some properties of an cc-amylase from potato tubers(Solanum tuberogum cv. Bintje) and thermostable bacterial a-amylase 366D Todorova and MA. Vijayalakshmi

SMB Separation of Dextran-Fructose Mixtures Produced by Leuconostocmesenteroides NRRL B512(0 367Mariana Santos, Jose Teixeira and All no Rodrigues

Selectivity of Commercial Culture Media for the Endogenous Microflora of TraditionalSourdough Bread 368JoSo M. Rocha and F. Xavier Malcata

Fed-batch Baker's Yeast Production Influence of Nitrogen Feeding on Product Quality 369K Schmidt and C. Posten

Transient Response of Saccharomyces cerevisiae to change in the rate of substratesupply 370P Duboc and U. von Stockar

AUTHOR INDEX 3 7 1

xxu