Probiotics containing complex vegetable fermented fibers (CVFF) enhanced human intestinal...
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Probiotics containing complex vegetable fermented fibers (CVFF) enhanced human intestinal microflora. Hsin-Ju Chou 12 , Yasuhiro Nakayama 2 , Ya-Yuan Chuang 2 , Hui-Fang Shang 3 ,Hui-Ting Yang 4 , and Shih- Yi Huang 1 1 School of Nutrition and Health Sciences, 2 MORIKAWA (Taiwan) Company Ltd, 3 Department of microbiology, Taipei Medical University, Taipei, Taiwan, I-Shou University, Kaohsiung, Taiwan Abstract Abstract To investigate the effectiveness of combined supplementation of vegetable fermented fibers and L. rh amnosus GG (LGG), a crossover study was designed for evaluating gastroenterological function in healthy subjects. After one week of dietary adjustment, all subjects took 10 tablets of complex vegetable ferm ented fibers everyday for 3 weeks. After two weeks of washout period, subjects took 10 tablets of CVF F (320mg crude fiber per tablet) plus LGG (8 × 10 7 CFU per tablet) for successive three weeks. Numbers of fecal Bifidobacterium spp., L. rhamnosus GG, and Lactobacillus spp. were determined weekly. Total anaerobic bac teria contents (TABC) and Clostridium Perfringens (CP) were assayed as well. A self-conscious questionnaire of gastroenterological condition was used to evaluate palatability and acceptability of subjects. Resu lts showed health volunteers administrated with 10 tablets of CVFF for three weeks indeed increased th e numbers of Bifidobacteria and LGG significantly and may improve intestinal microflora. However, CVFF plu s LGG accelerated the progress and further decreased CP and TABC in the first week. In conclusion, the study demonstrated that combined use of vegetable fermented fibers and probiotics could improve intest inal microflora in human body without any side effect. Table 1 、 Eeffects of CVFF on fecal miroflora ch anges Week1 Week2 Week3 Week4 Week5 Log 10 CFU/g wet weight of feces Tota l 9.50±0.20 a 10.04±0.15 c 9.65±0.21 ab 9.90±0.29 bc 9.81±0.58 bc L 6.82±1.31 a 7.42±1.30 ab 7.79±1.06 b 8.02±1.05 b 8.28±0.87 b B 8.93±0.38 a 9.36±0.39 c 9.02±0.37 ab 9.29±0.30 bc 9.30±0.31 bc LGG 6.18±1.11 a 7.27±1.06 b 7.18±0.87 b 7.19±0.99 b 6.93±1.26 ab Clos. 5.08±1.25 a 4.52±1.03 ab 4.31±1.17 ab 3.88±1.12 b 4.46±1.14 ab ere expressed as MEAN log CFU/gram of wet weight of feces ± SD. n=12. in the same row with different superscript letters were significantly different(p < 0.05). total anaerobic bacteria counts; L: Lactobacillus spp.; B: Bifidobacterium. spp.; C.: Clostridium perfringen ： dietary adjustment 、 Week 2~ Week 4 ： experimental period 、 Week 5 ： empty Figure 1. Effects of CVFF on counts of intes tinal anaerobic microflora Week 1 ： dietary adjustment 、 Week 2~ Week 4 ： exper imental period 、 Week 5 ： empty period (n=12) Figure 2.Change of total counts on intestinal Lactoba ccillus spp. Week 1 ： dietary adjustment 、 Week 2~ Week 4 ： experimen tal period 、 Week 5 ： empty period (n=12) Figure 3. Change of total counts for Bifidobacteri um. spp. Week 1:dietary adjustment 、 Week 2~ Week 4 ： experimental period 、 Week 5 ： empty (n ＝ 12) Figure 4. Effects of fermented fiber tablets on feca l total counts of L. rhamnosus GG. Week 1 ： dietary adjustment 、 Week 2~ Week 4 ： experime ntal period 、 Week 5 ： empty period (n=12) Figure 5. Effects of fermented fiber tablets o n intestinal counts of Clostridium perfringens. Week 1 ： dietary adjustment 、 Week 2~ Week 4 ： experiment al period 、 Week 5 ： empty period (n=12) Results Results L.rham nosus G G 5.60 5.90 6.20 6.50 6.80 7.10 7.40 7.70 8.00 1 2 3 4 5 w eek Log10 C FU /g w et w eightfeces LGG纖 纖 纖 a b b b ab ◆ CVFF Clostridium perfringens 2.50 3.00 3.50 4.00 4.50 5.00 5.50 1 2 3 4 5 w eek Log10 C FU /g w etw eight feces LGG纖 纖 纖 ab b ab ab a ◆ CVFF Clostridium perfringens. Lactobacillusspp. 5.50 6.00 6.50 7.00 7.50 8.00 8.50 9.00 1 2 3 4 5 w eek Log10 C FU /g w et w eightfeces LGG纖 纖 纖 a ab b b b ◆ CVFF Bifidobacterium spp. 7.00 7.50 8.00 8.50 9.00 9.50 10.00 10.50 1 2 3 4 5 w eek Log10 C FU /g w et w eightfeces LGG纖 纖 纖 bc bc ab c a ◆ CVFF Totalanaerobic organism s 8.00 8.50 9.00 9.50 10.00 10.50 11.00 1 2 3 4 5 w eek Log10 C FU /g w etw eight feces LGG纖 纖 纖 a ab c bc bc ◆ CVFF
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Transcript of Probiotics containing complex vegetable fermented fibers (CVFF) enhanced human intestinal...
Probiotics containing complex vegetable fermented fibers (CVFF) enhanced human intestinal microflora.
Hsin-Ju Chou12, Yasuhiro Nakayama2, Ya-Yuan Chuang2, Hui-Fang Shang3,Hui-Ting Yang4, and Shih-Yi Huang1
1School of Nutrition and Health Sciences, 2MORIKAWA (Taiwan) Company Ltd, 3Department of microbiology, Taipei Medical University, Taipei, Taiwan, I-Shou University, Kaohsiun
g, Taiwan
AbstractAbstract To investigate the effectiveness of combined supplementation of vegetable fermented fibers and L. rhamnosus GG (LGG), a crossover study was designed for evaluating gastroenterological function in healthy subjects. After one week of dietary adjustment, all subjects took 10 tablets of complex vegetable fermented fibers everyday for 3 weeks. After two weeks of washout period, subjects took 10 tablets of CVFF (320mg crude fiber per tablet) plus LGG (8 × 107 CFU per tablet) for successive three weeks. Numbers of fecal Bifidobacterium spp., L. rhamnosus GG, and Lactobacillus spp. were determined weekly. Total anaerobic bacteria contents (TABC) and Clostridium Perfringens (CP) were assayed as well. A self-conscious questionnaire of gastroenterological condition was used to evaluate palatability and acceptability of subjects. Results showed health volunteers administrated with 10 tablets of CVFF for three weeks indeed increased the numbers of Bifidobacteria and LGG significantly and may improve intestinal microflora. However, CVFF plus LGG accelerated the progress and further decreased CP and TABC in the first week. In conclusion, the study demonstrated that combined use of vegetable fermented fibers and probiotics could improve intestinal microflora in human body without any side effect.
Table 1 、 Eeffects of CVFF on fecal miroflora changes
Week1 Week2 Week3 Week4 Week5
Log 10 CFU/g wet weight of feces
Total 9.50±0.20 a 10.04±0.15 c 9.65±0.21 ab 9.90±0.29 bc 9.81±0.58 bc
L 6.82±1.31 a 7.42±1.30 ab 7.79±1.06 b 8.02±1.05 b 8.28±0.87 b
B 8.93±0.38 a 9.36±0.39 c 9.02±0.37 ab 9.29±0.30 bc 9.30±0.31 bc
LGG 6.18±1.11a 7.27±1.06 b 7.18±0.87 b 7.19±0.99 b 6.93±1.26 ab
Clos. 5.08±1.25 a 4.52±1.03 ab 4.31±1.17 ab 3.88±1.12 b 4.46±1.14 ab
Data were expressed as MEAN log CFU/gram of wet weight of feces ± SD. n=12.Values in the same row with different superscript letters were significantly different(p < 0.05).Total: total anaerobic bacteria counts; L: Lactobacillus spp.; B: Bifidobacterium. spp.; C.: Clostridium perfringensWeek 1 ： dietary adjustment 、 Week 2~ Week 4 ： experimental period 、 Week 5 ： empty
Figure 1. Effects of CVFF on counts of intestinal anaerobic microflora
Week 1 ： dietary adjustment 、 Week 2~ Week 4 ： experimental period 、 Week 5 ： empty period (n=12)
Figure 2.Change of total counts on intestinal Lactobaccillus spp.
Week 1 ： dietary adjustment 、 Week 2~ Week 4 ： experimental period 、 Week 5 ： empty period (n=12)
Figure 3. Change of total counts for Bifidobacterium. spp.
Week 1:dietary adjustment 、 Week 2~ Week 4 ： experimental period 、 Week 5 ： empty (n ＝ 12)
Figure 4. Effects of fermented fiber tablets on fecal total counts of L. rhamnosus GG.
Week 1 ： dietary adjustment 、 Week 2~ Week 4 ： experimental period 、 Week 5 ： empty period (n=12)
Figure 5. Effects of fermented fiber tablets on intestinal counts of Clostridium perfringens.
Week 1 ： dietary adjustment 、 Week 2~ Week 4 ： experimental period 、 Week 5 ： empty period (n=12)
ResultsResults
L. rhamnosus GG
5.605.906.206.506.807.107.407.708.00
1 2 3 4 5week
Log
10 C
FU/g
wet
wei
ght fe
ces
LGG纖維錠
a
b b bab
◆ CVFF
Clostridium perfringens
2.50
3.00
3.50
4.00
4.50
5.00
5.50
1 2 3 4 5week
Log10 C
FU
/g w
et w
eight
fece
s
LGG纖維錠
abb
abab
a
◆ CVFF
Clostridium perfringens.
Lactobacillus spp.
5.50
6.00
6.50
7.00
7.50
8.00
8.50
9.00
1 2 3 4 5week
Log
10 C
FU/g
wet
wei
ght fe
ces
LGG纖維錠
aab
b bb
◆ CVFF
Bifidobacterium spp.
7.00
7.50
8.00
8.50
9.00
9.50
10.00
10.50
1 2 3 4 5week
Log
10 C
FU/g
wet
wei
ght fe
ces
LGG纖維錠
bcbcabc
a
◆ CVFF
Total anaerobic organisms
8.00
8.50
9.00
9.50
10.00
10.50
11.00
1 2 3 4 5week
Log
10 C
FU/g
wet
wei
ght
fece
s
LGG纖維錠
aab
c bc bc
◆ CVFF
