Protein structure

Organizational level

• The structure of the protein molecule includes four organizational levels.

• 1) primary

• 2)secondary

• 3)tertiary

• 4)quaternary

Primary structure

• Primary structure of protein is defined as

• the sequence of amino acids .

• Importance:

• 1)detection of genetic diseases

• 2)Improper folding

• 3)loss or impairment of normal function

Peptide bond

• The peptide bonds are amide linkages

• Between two amino acids in which the

• Alpha carboxyl group of one amino acid is attached to the alpha amino group of the other amino acid.

• Example..valine and alanine form dipeptide valylalanine.

Breaking of peptide bond

• Unlike proteins which are denatured by conditions such as :

• 1)Heating• 2)High concentration of urea• Peptides are broken by :prolonged

exposure to :• Strong acid or base at elevated

temperature.This hydrolyzes these bonds non-enzymically.