Presented by Rachel Barlow
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Transcript of Presented by Rachel Barlow
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Study to Refine Comal Springs Riffle Beetle Collection Methods and
Establish Riffle Beetle Population Distribution
Presented by Rachel Barlow
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According to the EAA Request for Proposals (RFP) for this study:Comal Springs riffle beetle (CSRB) distribution is currently
unknownCSRB collection methods can be improved
Per the RFP, this study is intended to:Establish a distribution of springs serving as CSRB habitatTest the success rate of at least three collection methodsCollect CSRB for use in other 2014 Applied Research studies
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425 mapped springs in Landa Lake and the Comal System (Norris and Gibson 2013)
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Currently Understood CSRB Distribution within the Comal System
Western shorelineOff northeastern tip of Spring IslandBetween western shoreline and northernmost peninsula
of Pecan IslandSpring runs 1-3
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BIO-WEST 2002
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BIO-WEST 2002
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Previous Collection Methods
Fingers and/or soft forcepsDip netsCotton cloth luresCotton lures inside PVC
tubingDrift net sampling
Cotton cloth lure allowed to “culture” in situ for a period of four weeks
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Study ObjectivesTest the success rate of three lure typesEstablish CSRB distribution within the Comal System
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Methodology
Preliminary trials with surrogate speciesCollect 250 CSRB (maximum)Store in aquaria
Beetle Collection and Housing
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Methodology (con’t)
Four experimental chambers- Three test systems- Control
Treatments (24 hour test cycle x 3)- Cotton cloth lure- Lighted cotton cloth lure - Hemp cloth lure
50 CSRB per chamberExpected results and analysis
- % CSRB captured per test cycle- ANOVA (lure type)
Laboratory Experimental Design
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Methodology (con’t)
Adaptive sampling- Only sample springs with CSRB habitat- CSRB present, move on
Exclude springs with known CSRBUse most effective lure realized in laboratory studySurvey (check traps) up to 3 times Survey no more than twice weekly
Population Distribution Study
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CSRB Habitat Criteria(based on physical parameters measured by Norris and Gibson 2013)
Spring type: upwellingTemperature: 68-75°FSubstrate consisting of organics, sand, course sand,
very small gravel, small gravel, medium gravel, rubble, small cobble, and large cobble
Flow sufficient to maintain clear substrate conditions
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Expected Results
Tested collection methodUpdated map of CSRB distributionAnimals per trap across spring orificesExamination of other contributing parametersNumber of surveys leading to presence/absence
determinationNature of changing spring locations in relation to
presence/absence studies
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