Presentation proposal eel 2003

8/14/2019 Presentation proposal eel 2003

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Faculty of Biotechnology and Life Sciences

Research Proposal

IDENTIFICATION ANDISOLATION OF PROBIOTICMICROORGANISM IN EEL

Name : Farhanna Mohammed

Matrix no : 870131565028

Course : Bachelor of Biotechnology Industry

Supervisor : Miss Suwaibah Mohamed


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INTRODUCTIONTHE EELS FACT


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PROBIOTIC BACTERIAFACT


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OBJECTIVE OF THERESEARCH


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HYPOTHESIS AND EXPECTED

RESULT


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LITERATURE REVIEW


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METHOD

CONVENTIONAL METHOD

MOLECULARMETHOD

Grams

staining

Catalase test

MethylRedtest(MR)

PCR


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Lactic acid producing bacteria (probiotic

bacteria) isolated from eel digestive tract

The eel fish was collected

from fresh water river.

pour plated on

nutrient agar plate

and incubated for 24

h at room

temperature.

After centrifugation the

supernatant was taken

and serially diluted in

sterile distilled water in

the test tubes to 10-2, 10-

3, 10-4, 10-5, 10-6, 10-7 and

10-8

Individual colonies

were taken and

inoculated in

McConkey broth.

Then the

broths were

incubated for

18-24 h at

37C.

The single

colony is

subject to lab

identification

The digestive tracts

were homogenized in

peptone broth for

centrifugation.

Then the animals were

dissected to remove

the digestive tracts by

the sterilization

condition.

The eel fish were

washed with sterile

distilled to remove the

unwanted particles.


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GRAM STAINING


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CATALASE TEST


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PCR IDENTIFICATION


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DATA COLLECTION

Data will be collected from February 2009 until November 2009. Qualitative

data will be collected via journal and internet research or any other media.

The qualitative data is more on focusing into literature review and previous

research on the same study.

For the quantitative data, they will be collected from the lab experiment

which will be conducted during the research. It will include morphological

analysis, biochemical analysis and Polymerase Chain reaction (PCR)

analysis. The data will be collected from at least 20 samples to get the exact

result.


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Item Cost

Live eel RM20.00/kg

Medium (Mc Conkey) RM 250.00

Other necessary chemical and apparatus

which may not available in lab

RM 150.00

Taq buffer RM250.00

MgCl2 RM250.00

dNTPs RM250.00

Forward primer RM250.00

Reverse primer RM250.00

Taq polymerase RM250.00

Grand Total RM1920.00

COSTING AND EXPENSES

Item Cost

Live eel RM20.00/kg

Medium (Mc Conkey) RM 250.00

Other necessary chemical and

apparatus which may notavailable in lab

RM 150.00

Taq buffer RM250.00

MgCl2 RM250.00

dNTPs RM250.00Forward primer RM250.00

Reverse primer RM250.00

Taq polymerase RM250.00

Grand Total RM1920.00


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TIME FRAME

ITEM FEB MAR APR MAY JUNE JULY AUG SEP OCT NOV

LITERATURE

REVIEW

DATA

COLLECTION

LAB WORK

DATA

ANALYSIS

THESIS

WRITING

THESIS

SUBMITTION


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CONCLUSION

This research willreveal particular

probioticmicroorganism in

eel fish and theirbenefits to thespecies will be

highly and clearlyunderstood.

This research alsowill determinewhat kind of

probiotic bacteriathat can inhibit

other pathogenicmicroorganisms in

eel fish.

Presentation proposal eel 2003

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