Tissue Engineering of Nasal Septum Using Chitosan- Based Material

by Arunkumar rengaraj

(22141800)

Nasal septum

Nasal septum divides the nasal chamber into two cavities.

The turbinates are made of bone and soft tissue.

Conventional repair of nasal septum involves autologous rib or septum cartilage grafts and prosthetic devices.

Its having limitations like tissue availability, and prosthesis related infection and extrusion.

Major reason leading to the failure

Lack of sufficient control over shape is the lack of appropriate scaffolds

Less biocompatibility for cell seeding

Chitosan

• Chitosan, a linear polysaccharide derived from crustacean shells and from many natural sources.

• It having advantages like polycationic nature, biodegradability, biocompatibility, mucoadhesiveness.

• In addition, it enhances epithelial permeability through the opening of tight junctions between epithelial cells.

Glycosaminoglycans

It is long unbranched polysaccharides.

GAGs in enhancing chondrogenesis in vitro.

One of the chief components of the extracellular matrix .

It contributes significantly to cell proliferation and migration.

Hyaluronan recognizing receptor

• CD44

• Receptor for HA-mediated motility (RHAMM)

• Intercellular adhesion molecule-1 (ICAM-1)

It may also be used postoperatively to induce tissue healing.

Materials and methods

2 % solution of chitosan + 1 M acetic acid

Hybrid polymer

Add Hyaluronic acid/ MeOH 1:1Mix 40 μg type I collagen

Tissues (Human inferior turbinates ) were treated with 0.5% pronase+1:1 mixture (DMEM) +

Ham’s nutrient F12

centrifugation

suspended in DMEM/F12

1.5-mL cell suspension was seeded on collagen coated

Morphological Examination

• Calculate the pore size of the mess

• The DNA content of the cell have to study for calculating the proliferation.

• The ciliary beat was observed under inverted microscope (Leica, DMI 6000, Solms, Germany) with 400 magnification.

• Imaging of cilia movement was recorded at 240 frames per second with a high-speed complementary metal oxide

semiconductor (CMOS) camera (CMC1300; VDS Vossku¨ hler GmbH, Osnabru¨ ck, Germany).

• Histological and immuno-histochemical apperance of the tissue.

• Finally want to perform animal study.

Reference

• Dong-Won Lee, Shawna A Shirley, Richard F Lockey and Shyam Mohapatra. Thiolated chitosan nanoparticles enhance anti-inflammatory effects of intranasally delivered theophylline. Respiratory Research 2006; 7:112.

• Tsung-Wei Huang, Yi-Ho Young, Po-Wen Cheng, Yen-Hui Chan, Tai-Horng Young. Culture of Nasal Epithelial Cells Using Chitosan-Based Membranes. The Laryngoscope 2009; 119:2066-2070.