TEAM TU-EINDHOVEN

MRI AND

BACTERIA AS

DIAGNOSTIC

TOOLS

THE ROAD TO OUR SOLUTION

TUMOR IMAGING

MRI scan of latter stages of brain cancer

Photograph by Zephyr/Science Photo Library

2007 National Geographic Society

CHEMICAL EXCHANGE SATURATION TRANSFER

ORGANIC CONTRAST AGENTS

CHEMICAL EXCHANGE SATURATION TRANSFER

ORGANIC CONTRAST AGENTS

CHEMICAL EXCHANGE SATURATION TRANSFER

ORGANIC CONTRAST AGENTS

CHEMICAL EXCHANGE SATURATION TRANSFER

ORGANIC CONTRAST AGENTS

CHEMICAL EXCHANGE SATURATION TRANSFER

ORGANIC CONTRAST AGENTS

CHEMICAL EXCHANGE SATURATION TRANSFER

ORGANIC CONTRAST AGENTS

THE ROAD TO OUR SOLUTION

BACTERIA BASED CANCER THERAPIES

Our Solution

TEAM TU-EINDHOVEN

TEAM TU-EINDHOVEN

The Sensor

The Chassis

Contrast

Agents

Safety

Sense the hypoxic environment

Generate contrast on an MRI scanner

Integrate the sensor and contrast agents

Ensure patient safety

The Sensor

TEAM TU-EINDHOVEN

Safety The Chassis Contrast

Agents The Sensor

TEAM TU-EINDHOVEN

The Sensor

Safety The Chassis Contrast

Agents The Sensor

Shift to low oxygen concentration

TEAM TU-EINDHOVEN

The Sensor

Safety The Chassis Contrast

Agents The Sensor

INTENSITY

INCREASES OVER

TIME

TEAM TU-EINDHOVEN

ANAEROBIC EXPRESSION

The Sensor

Safety The Chassis Contrast

Agents The Sensor

// High Lysine Ratio

TEAM TU-EINDHOVEN

Contrast Agents

Define

chassis Safety The Chassis

Contrast

Agents The Sensor

1PJN

// Practicality

// Molecular

Dynamics

Simulations

TEAM TU-EINDHOVEN

// 1ETF

// 1G70

// 1PJN

// Human Protamine 1

// Poly Arginine-Glycine

// Poly Arginine-Serine

// Poly Threonine-Lysine

// Poly Lysine-Serine

Define

chassis Safety The Chassis

Contrast

Agents The Sensor

THE SELECTION

Contrast Agents

AEROBIC EXPRESSION

NATIVE PROTEINS ARE CLEARLY EXPRESSED

TEAM TU-EINDHOVEN

Contrast Agents

Define

chassis Safety The Chassis

Contrast

Agents The Sensor

TEAM TU-EINDHOVEN

Contrast Agents

Define

chassis Safety The Chassis

Contrast

Agents The Sensor

Proteins with a high Lysine content

MRI RESULTS

TEAM TU-EINDHOVEN

Contrast Agents

Define

chassis Safety The Chassis

Contrast

Agents The Sensor

MRI RESULTS

THE BIOBRICKS

TEAM TU-EINDHOVEN

The Chassis

19 NEW BIOBRICKS:

- A FUNCTIONAL ANAEROBIC PROMOTER

(BBa_K1123000)

- 9 CEST PRODUCING PROTEINS

(BBa_K1123013 – BBa_K1123021)

- 9 COMPLETE TUMOR VISUALIZATION CONSTRUCTS

(BBa_K1123001 – BBa_K1123009)

Safety The Chassis The Sensor Contrast

Agents

The Safety Locks

TEAM TU-EINDHOVEN

AVOIDING AN IMMUNE

SYSTEM RESPONSE

Define

chassis Safety The Chassis The Sensor

Contrast

Agents

1. J.C. Anderson, E.J. Clarke, A.P. Arkin, and C.A. Voigt, Environmentally Controlled Invasion of Cancer Cells by Engineered Bacteria. Molecular Biology 355, 619-627 (2006)

2. K. Gao, E. coli: The Cure for Cancer?. The Triple Helix , 12-13 (2010)

The Safety Locks

TEAM TU-EINDHOVEN

THE KILL SWITCH

Define

chassis Safety The Chassis The Sensor

Contrast

Agents

TEAM TU-EINDHOVEN

THE KILL SWITCH MODEL

Define

chassis Safety The Chassis The Sensor

Contrast

Agents

The Safety Locks

BACTERIA AS

DIAGNOSTIC TOOLS: WILL SOCIETY ACCEPT

THE IDEA?

// Bringing the world of synthetic biology

into everybody’s home.

// Removing the reservations people

have about synthetic biology by

removing faulty data.

// Collaborating and collecting data

across the globe.

TEAM TU-EINDHOVEN

SYNTHETIC FACTS

Human Practices

TEAM TU-EINDHOVEN

SYNTHETIC FACTS

Human Practices

SYNTHETIC FACTS

21 Initial Facts To Be Checked.

12 Main Topics.

10 Facts Are Officially Checked, Uploaded And Available.

TEAM TU-EINDHOVEN

Human Practices

SyntheticFacts.tue.nl

FINAL

CONCLUSIONS

TEAM TU-EINDHOVEN

We have established

the foundations for a

new bacterial

application.

“Synthetic Facts” will

continue to give

reliable and unbiased

information.

TEAM PICTURE!!! Special Thanks To… // dr. Maarten Merkx

// dr. ir. Tom de Greef

// dr. ir. Natal van Riel

// ir. Stijn Aper

// Sagitta Peters MSc.

// Industrial Designer Mariana Felix

// ir. Stephanie Hectors

// prof.dr. Klaas Nicolaij

TEAM TU-EINDHOVEN

Acknowledgements