Name Surname Institution Expertise

Chiraz Frydman Horiba SPRi

David Pantoja IQFR-CSIC, Madrid NMR

Dmitry Svergun EMBL c/o DESY

(Hamburgo, DE)

Small Angle X-ray Scattering

Erdinc Sezgin

MRC Human Immunology Unit (Oxford University)

Fluorescence Microscopy

Eva Muñoz Affinimeter Analysis and simulation software

Irene Díaz-Moreno IIQ, Universidad de Sevilla-CSIC, cicCartuja

NMR

Marlène Martinho Université Aix Marseille – CNRS

Spin Labeling, EPR

Pedro M. Nieto IIQ, Universidad de Sevilla-CSIC, cicCartuja

NMR

Peter Vikegard Waters Sverige AB Calorimetry

Sergio Gil-Caballero IIQ, Universidad de Sevilla-CSIC, cicCartuja

NMR

Valérie Belle Université Aix Marseille – CNRS

Spin Labeling, EPR

Federation of European Biochemical Societies A charitable organization advancing research in the

molecular life sciences across Europe and beyond.

www.febs.org

Currently, the quest for novel biomolecular interaction

networks relies on multidisciplinary and high-throughput

biochemical, biophysical and computational approaches.

In this practical course, experts within the field will

introduce young researchers to the analysis of protein–

ligand and protein–protein interactions by lectures, open

discussion sessions and hands-on training experiments

using Nuclear Magnetic Resonance, MALDI-TOF, Spin

Labelling, Calorimetry, Dynamic Light Scattering, SPRi

and complementary calculative analyses.

biomolecularinteractions.febsevents.org

FEBS Practical Course on Integrated Approaches to Biomolecular Interactions

May 7-11, 2018 / Sevilla, Spain

FEBS 2018 ADVANCED COURSE

ORGANIZING COMMITTEE

SPEAKERS PROGRAM FEATURES

The course will be focus on the following techniques:

NMR (Nuclear Magnetic Resonance)

Protein-observer experiments to determine the location

of the binding site and estimation of the Kd according to

Chemical Shift Perturbation (CSP), Paramagnetic

Relaxation Enhancements (PRE) and several ligand-

observed experiments as tr-NOESY, STD and Water

LOGSY will be performed and compared to achieve real

understanding of the different methods. Processing and

analysis of the acquired experiments will be also

performed in parallel sessions.

MALDI-TOF (Soft Ionization Mass Spectroscopy)

Analysis of biomolecular standards and measurement of

the stoichiometry of non-covalent protein-protein

interaction will be performed.

Spin Labelling

Standard spin labelled samples will be prepared to

measure Paramagnetic Relaxation Enhancements, PRE,

by NMR.

ITC (Isothermal Titration Calorimetry)

Calorimetric measurements of binding constants of

protein-protein and protein-ligand interactions will be

performed as well as protein stability analyses upon

ligand binding by Differential Scanning Calorimetry, DSC.

DLS (Dynamic Light Scattering)

Sample test by Dynamic Light Scattering, DLS, will be

performed and analyzed.

SPRi (Surface Plasmon Resonance)

Surface Plasmon Resonance,SPRi, will be performed

using standard protein-DNA samples and sensorgram

analysis will be performed.

Complementary techniques as EPR, Electron Microscopy

and SAXS will be also introduced as lectures during the

course.

COURSE ORGANIZERS

Antonio J. Díaz-Quintana IIQ, Universidad de Sevilla-CSIC, cicCartuja

David Pantoja IQFR-CSIC, Madrid

Pedro M. Nieto IIQ, Universidad de Sevilla-CSIC, cicCartuja

Sergio Gil-Caballero IIQ, Universidad de Sevilla-CSIC, cicCartuja

Valérie Belle Université Aix Marseille – CNRS

ADVISORY COMMITTEE

Irene Díaz-Moreno IIQ, Universidad de Sevilla-CSIC, cicCartuja

Miguel A. De la Rosa IIQ, Universidad de Sevilla-CSIC, cicCartuja

FEBS MEMBER IN CHARGE

Stefana Petrescu Institute of Biochemistry. Bucarest.

May 7-11, 2018 / Sevilla, Spain

FEBS 2018 ADVANCED COURSE

PROGRAMME

During an initial general introduction, the schedule will be explained and the students will be assigned to different teams. Given

the background diversity of the attendees, several introductory lectures will aid the participants to focus on their technical

aspects during training. The course will focus on the following techniques: NMR, MALDI-TOF, Spin Labelling, Calorimetry,

Dynamic Light Scattering, SPRi and data processing.

VENUE FOR THE SCIENTIFIC PROGRAMME All the necessary equipment and techniques are available at

the recently established Biomolecular Interaction Platform

(BIP) at the cicCartuja research centre, located near the

historical centre of Seville.

Participants will

be accommodated

in the Ribera de

Triana Hotel

ACKNOWLEDGEMENTS

BURSARIES FEBS YTF AWARDS

A limited number of FEBS Youth Travel Funds (YTF) Awards

are available. These funds will cover costs of registration,

shared accommodation in a double room, travel expenses

and meals.

EBSA GRANTS

In accordance with the aims of European Biophysical

Societies' Association (EBSA) to foster and support training

in Biophysics in Europe, six bursaries will be awarded to

young scientists from EBSA affiliated countries outside of

Spain.

Time May 6, Sunday

May 7, Monday

May 8, Tuesday

May 9, Wednesday

May 10, Thursday

May 11, Friday

May 12, Saturday

9.00-9.30

Arrival

General introduction

SPIN-LABEL

NMR1

MALDI

NMR2 SPIN-LABEL

PROC1

PROC2

MALDI

NMR2

MALDI

NMR3

PROC2

Departure

9.30-10.30 Antonio

Díaz Quintana ES

10.30-11.30 Peter Vikegard

TA Instruments SW

11.30-12.30

Eva Muñoz Affinimeter modelling

ES

12.30-13.00 Break

13.00-13.45 Valérie Belle

FR

Chiraz Frydman

Horiba

FR

Erdinc Sezgin

EN

Pedro Nieto

ES

Irene Díaz-Moreno

ES

13.45-14.30 Sergio Gil

ES

Dmitry Svergun

GE Discussion 1 Discussion 2 Discussion 3

14.30-15.30 Lunch

15.30-19.00

NMR-1

ITC/DSC/DLS

SPIN LABELS

ITC/DSC/DLS

SPRi

NMR1

PROC1

NMR2

SPRi

SPRi

PROC1

NMR3

NMR3 PROC2

ITC/DSC/DLS

20:30 Welcome

Tapas Dinner Course dinner

Social event