Prepared by Becky Jimenez NSF REU Civil Infrastructure...
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Prepared by Becky Jimenez NSF REU Civil Infrastructure Engineering, UH, Summer 2009
Transcript of Prepared by Becky Jimenez NSF REU Civil Infrastructure...
Prepared by Becky Jimenez
NSF REU Civil Infrastructure Engineering, UH, Summer 2009
� Background and Theory
� Site Details
� Bacteria
� Lab Processing� Lab Processing
� Analysis
� Conclusion
� Total Coliform and E.coli concentrations higher in
sediment than water
o Decreasing Sunlight Inactivation
o Protection from predationo Protection from predation
o Increased nutrient availability
o Colonizable surface
E. COLI
� Total Coliforms
� Fecal indicator bacteria
(FIB)(FIB)
o E. coli
YSI READINGS UV READINGS
Lab Lab
ProcessingProcessing� Matching samples according to time
�Dilutions (Different for Sediment)
�Adding reagent
�Shaking
�Placed into Quanti-trays and Sealed
24 Hour Incubation at 35 degrees � 24 Hour Incubation at 35 degrees
Celsius
WATER SAMPLES SEDIMENT SAMPLES
Requirements:
• Geometric Mean < 126 MPN/dL
• Individual Samples cannot exceed 394 MPN/dL more than 25% of the time
2000
2500
3000
Co
nce
ntr
ati
on
(M
PN
/dL) Geometric Mean
0
500
1000
1500
2002 2003 2004 2005 2009E.
co
li C
on
cen
tra
tio
n (
MP
N/d
L)
Years
Geometric mean of E. coli concentrations for different years
Cole Creek
Samples
Collected
Samples > 394
MPN/__ % Exceeding Standard
Geometric
Mean
Water
(MPN/dL) 24 15 62.5 441
Sediment
(MPN/kg) 24 24 100 110470
100limit over samplesof number
standardexceeding % ×=
CONCENTRATION IN: TOTAL COLIFORM E. coli
SEDIMENT (MPN/kg)
MINIMUM 238200 7100
MAXIMUM 24196000 1670000
WATER (MPN/dL)
MINIMUM 19177 147
MAXIMUM 61410 2894
Maximum and minimum
E. coli and total coliform
concentrations
100evaluated samplestotal number
limit over samplesof number standardexceeding % ×=
TOTAL COLIFORM
CONCENTRATION IN
WATER CHANGE
WITH TIME
FLUCTUATION OF
TOTAL COLIFORM
CONCENTRATION
IN SEDIMENT
VARIABILITY OF E. COLI
CONCENTRATION IN
WATER
FLUCTUATION OF E. COLI
CONCENTRATION IN
SEDIMENT
y = 1245.3e-2.38x
R² = 0.666
0
200
400
600
800
6:00 AM 9:00 AM 12:00 PM 3:00 PM 6:00 PM
E. c
oli
co
nce
ntr
ati
on
(MP
N/d
L)
Time
Time and E. coli Expon. (Time and E. coli)
Conclusions and Questions
The research study described herein was sponsored by the National
Science Foundation under the Award No. EEC-0649163. The opinions
expressed in this study are those of the authors and do not necessarily
reflect the views of the sponsor.
I would also like to thank Dr. Hanadi Rifai, Anu Desai, Sean Carbonaro,
Norma Moreno, Scott Rauschhuber, Yaa Amoah, Sharon Wells, Nathan Norma Moreno, Scott Rauschhuber, Yaa Amoah, Sharon Wells, Nathan
Howell, Jenni McFarland, Lisa Grecho, Maria Modelska, Stephen Ray,
Zack Van Brunt, Matt Feaga and Divagar Lakshmanan.
