Preparation of Active Pharmaceutical Ingredients (API) by ... · (API) by Continuous Processing....
Transcript of Preparation of Active Pharmaceutical Ingredients (API) by ... · (API) by Continuous Processing....
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B R I A N M A R Q U A R D TW E S T H O M P S O NA P P L I E D P H Y S I C S L A B O R A T O R YU N I V E R S I T Y O F W A S H I N G T O Nm a r q u a r d t @ a p l . w a s h i n g t o n . e d u
Preparation of Active Pharmaceutical Ingredients
(API) by Continuous Processing
MEPIU.S. Food andDrug Administration
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Why Use Advanced Flow Reactors?
High Throughput Experimentation For…Discovery and screeningProcess developmentProcess optimizationProcess controlProduction?
Eliminate chemical engineering production problems related to scaling up batch systems?Increase production through use of many parallel microreactors to achieve volume?
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Challenges To Using AF Reactors
EDUCATION!!!!!!Interfacing modular unitsSampling and screeningAnalytical characterizationData handlingProcess modeling and feed back control (particularly if they are used for production)
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Example: Esterification of Methanol
Standard Raman spectra
Acetic Acid + Methanol Methyl Acetate + H2OH+
Raman Shift (cm-1)
Inte
nsity
Acetic AcidMethanol Methyl Acetate
0
5000
10000
15000
20000
400 600 800 1000 1200 1400 1600 1800
ROI
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• PCA analysis of the formation of acetate monitoredby Raman spectroscopy – reaction time 1.5 hours
0 30 600
50
100
150
200
250
Rel
ativ
e In
tens
ity
90Reaction Time (min.)
5º
15º25º35º
55º 45º65º
Batch Runs at Different Temperatures
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Continuous Rxn. with Temp. Step
• with residence time module• flow rate: 10.56 ml/min (residence time ~ 2.5 min)
acetic acid
methyl acetate
Temp 40°C
Temp 25°C (25°C – 40°C)
• With control of flow reactor parameters and analytics, fast optimization is possible
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• without residence time module• flow rate: 0.89 ml/min (residence time ~ 5 min)
PCA Analysis on data after mixing:1st PCA scores Increase in reaction yield after each temperature step
Product Yield vs. Temperature
Range = 30-60°C
• 2 weeks of batch data reproduced in less than three hours by continuous flow
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Result: Estimated Response SurfacesD
esira
bilit
y
50% Time - 50% Product• Optimum response
- H2SO4= 0.230 ml- Ratio= 1.35 Ac + 1.65 MeOH- Temp = 45ºC
temp=40.0
0 100 200 300 01
23
Ratio(x1000)
00.20.40.60.8
1
H2SO4
20% Time - 80% Product• Optimum response
-H2SO4= 0.258 ml -Ratio= 1.34 Ac+1.66 MeOH -Temp = 46ºC
temp=40.0
Ratio(X 1000)
0 100 200 300 01
23
00.20.40.60.8
1
H2SO4
80% Time - 20% Product• Optimum response
-H2SO4= 0.141 ml-Ratio= 1.45 Ac + 1.55 MeOH-Temp = 51.4ºC
temp=40.0
H2SO4 Ratio(x1000)
0 100 200 300 01
23
00.20.40.60.8
1
• continuous reactors w/analytics allow for fast optimization of design space
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Analysis of Continuous Reactors
Problems with performing online measurementsGas formation in sample linesTemperature change before reaching analyzerPhase change between reactor and analyzerSensor placement at optimal positionAutomated flow and pressure control
Most PAT problems are due to sampling not measurement deviceNeed better systems to sample processes
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Application of Sampling Systems To Microreactors
EXAMPLE OF BRINGING ANALYTICS TO A PROCESS AND THE CHALLENGES WITH INTEGRATING THEM
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What is NeSSI?• Industry-driven effort to
define and promote a new standardized alternative to sample conditioning systems for analyzers and sensors
Standard fluidic interface for modular surface-mount components
ISA SP76 Standard wiring and communications interfacesStandard platform formicro analytics
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What does NeSSI™ Provide
Simple “Lego-like” assemblyEasy to re-configureNo special tools or skills required
Standardized flow components“Mix-and-match” compatibility between vendorsGrowing list of components
Standardized electrical and communication (Gen II)“Plug-and-play” integration of multiple devicesSimplified interface for programmatic I/O and control
Advanced analytics (Gen III)Micro-analyzersIntegrated analysis or “smart” systems
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NeSSI Raman Sampling Block
• Parker Intraflow NeSSI substrate• Sample conditioning to induce backpressure to
reduce bubble formation and the heated substrate allows analysis at reactor conditions
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Reactor Feed 1
Reactor Feed 2
Product Stream
Real-timeCalibration
waste
prod
AnalyzerSuite
Pump 1
Pump 2
NESSI AF Reactor Sampling/Calibration
• Application of sampling systems and analytics to optimize and control AF reactor
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FDA Collaboration: Demonstrate the Concept of QbD
- Monitoring an advanced flow reactor (AF Reactor) using NeSSi sampling systems and Raman ballprobe sampling interfaces at various reactor points.
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CPAC/FDA/Corning AF Reactor
Goal: to improve reaction development and optimization through the use of continuous glass flow reactors, NeSSI and analyticsFunded by the FDA to demonstrate the benefits of improved reactor design, effective sampling and online analytics to increase process understanding (QbD)Partners: FDA, Corning, CPAC, Kaiser, ParkerQbD Project began November 2008Process Reactions – June 2009
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Corning Advanced Flow Reactor
Continuous reactions are ideal for product and process optimization/ understandingProvide predictable and reliable reaction performance, easily customizable and transferable to a production facility Application of reactor, sampling and analytics, demonstrates the physical concept of QbD Additional analytics easily coupled to reactor through NeSSI substrates
4 channel, Kaiser Optical Systems Rxn2 probes placed in reactor flow path at different points of the reaction
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Advanced Flow Reactor Chemistry
Organic Acid Chloride Organic Carbonate + dimer
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Raman Analysis of AF Reactor
3 4
1
2
• monitor reaction with 4 channel 785 nm Raman system• NeSSI sampling systems (1-4) equipped with Raman ballprobes• Online GC also used as post quench online analyzer (4)
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For each feed :
PressureMass Flow rateTemperatureDensity
Raman Spectroscopy measurements on Feeds, Pre‐quench product and Post‐quench productDensity and viscosity measurements were done for Chloroformate/Toluene and Butanediol/Pyridine
Process on line measurements
Chloroformate in Toluene
0,6
0,65
0,7
0,75
0,8
0,85
0,9
0,95
1
0 20 40 60 80
mass % 2EHCF
0,5
0,6
0,7
0,8
0,9
1
1,1
DensityViscosity (cp)
Pyridine in Butanediol
0,5
0,7
0,9
1,1
1,3
1,5
0 5 10 15 20mass % pyridine
20
30
40
50
60
70
80
Density
Viscosity (cp)
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Advanced Flow Reactor Images
Raman Probes
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NeSSI Sampling and Raman Probe Images
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NeSSI Ballprobe - Raman/NIR/UV
Matrix Solutions: www.ballprobe.com
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Design of Experiments Information
31 Experiments totalTemperature stepsReaction with no tolueneChanges in butanediol ratioChanges in pyridine ratioPropanediol instead of butanediolSimulated Reactor problems
Pump failureLess heat exchangePoor dilution of chloroformate
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Product Raman Peaks of Interest
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Standard Peaks of Interest (High cm-1)
Chloroformate
Dimer
Carbonate
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Standard Peaks of Interest (Low cm-1)
Chloroformate
TolueneDimerCarbonateToluene
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C F M _ R U N 1J U N E 9 T H , 2 0 0 9
DoE Tests 0 and 3
T° Feed A Feed BMass FlowA+B
Feed C T°Total Mass Flow
Reaction Choloformiate in toluene Pyridine in butanediol Acidic solution Quench
°C g/min wt% g/min wt%
Eq Mol Pyridine
Eq Mol Butaned
iol g/min g/min wt% °C g/min
Half stoechiometry test
Test 0 60 10.0 40 10.1 8.1 0.5 5.0 20.1 20.2 10 10 40.3
TemperatureTest 1 20 8.0 40.0 16.0 8.1 1.0 9.8 24.0 20.2 10.0 10 44.2Test 2 40 8.0 40.0 16.0 8.1 1.0 9.8 24.0 20.2 10.0 10 44.2
Test 3 60 8.0 40.0 16.0 8.1 1.0 9.8 24.0 20.2 10.0 10 44.2
Propanediol instead of butanediol
Test 15 60 8.0 30.0 16.0 8.1 1.3 15.5 24.0 20.2 10.0 10 44.2Test 16 85 8.0 30.0 16.0 8.1 1.3 15.5 24.0 20.2 10.0 10 44.2
Test 17 30 8.0 30.0 16.0 8.1 1.3 15.5 24.0 20.2 10.0 10 44.2
GC Results (%)
R-OH 2EHCF R-Cl Carbonate Dimer2.16 45.84 0.32 51.42 0.591.17 0.00 0.26 96.17 2.40
Abbreviation DefinitionsR-OH 2EHCF R-Cl
Alcohol formed after quench
Residual, unreactedchloroformate
Natural degradation product of chloroformate
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Reaction Easily Followed With Raman
Test 3 Test 0Toluene Flush
Ch. 1
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Peaks of Interest (High cm-1)
Dimer Chloroformate
Ch. 1GC Results (%)
Test R-OH 2EHCF R-Cl Carbonate Dimer0 2.16 45.84 0.32 51.42 0.593 1.17 0.00 0.26 96.17 2.40
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Peaks of Interest (Low cm-1)
Chloroformate
Toluene
Toluene
Ch. 1GC Results (%)
Test R-OH 2EHCF R-Cl Carbonate Dimer0 2.16 45.84 0.32 51.42 0.593 1.17 0.00 0.26 96.17 2.40
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PCA of fingerprint region, PC 1
Toluene peaks removed
Toluene Flush Toluene Flush
Test 0
Test 3
Scores Loadings
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Current Status
Data collected and organized14 days in Toulouse France
Analysis startedEvaluation of modeling protocols
PCA, MCR, ALS
Calibrate to GC results (PLS)Determine better chemistry for Phase 2
More chemical change in reaction spaceImplement more sensorsAcquire reactor at CPAC
Implement Models for Process Feedback Control
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Thanks
U.S. Food and Drug AdministrationMoheb NasrChristine MooreDavid MorleyErik Henrikson
Corning GlassPhilipe CazeCeline GuermerJérémy Jorda
ParkerMike Cost
Kaiser Optical SystemsIan LewisHervé LucasBruno Lenain
CPACUniversity of WashingtonApplied Physics Lab
La Maison Européenne des Procédés Innovants (MEPI )
Annelyse Conté MEPI
U.S. Food andDrug Administration