PREPARATION AND EVALUATION OF VARIOUS HERBAL HAIR OIL ...

www.wjpr.net Vol 8, Issue 7, 2019.

Madhulatha et al. World Journal of Pharmaceutical Research

1817

PREPARATION AND EVALUATION OF VARIOUS HERBAL HAIR

OIL FORMULATIONS

Boyapati Madhulatha* and Kotresh Yaligar

Assoc. Professor, GBN Institute of Pharmacy- Gbn Institute of Pharmacy Affiliated to

Jawaharlal Nehru Technological University, Hyderabad, Telangana, 501301.

ABSTRACT

The objective of present study involves preparation of various herbal

hair oil formulations usingamla, Hibiscus, Bringraj, Brahmi, Henna,

Termineliabelarica, Flaxseeds, Oats, Calendula, Neem, Tridax.

Evaluating physical parameters and testing the efficacy of oil by using

rabbits. The hair oil formulations were prepared by using two different

methods paste method and cloth pouch method. The different oils

namely PF1,PF2,PF3,DMF1,DMF2,DMF3 were subjected to test of

sensitivity, specific gravity, acid value, saponification value, pH and

biological evaluation. DMF3 followed by DMF2 were found to be more

significant when compared to other formulations. The hair initiation

was started on 10th

day which is comparable to that of minoxidil (Hair

initiation 12th

day). Excellent results of hair growth were seen in

formulations prepared by cloth pouch method. They hold the promise

of potent herbal alternative for minoxidil.

KEYWORDS: Amla, Hibiscus, Bringraj, Brahmi, Henna, Termineliabelarica, Flaxseeds,

Oats, Calendula, Neem, Tridax.

1. INTRODUCTION

In India herbal medicines are the principal form of medicines. In India around 6000

manufacturers for herbal medicines are available. According to WHO around 80% people of

the world uses herbal medicines.[1]

It is common in women and men also. This caused by stress, menopause, birthcontrol,

medication, changing hormones, and the plethora of hair styling products.[2]

Alopecia is a

World Journal of Pharmaceutical Research SJIF Impact Factor 8.074

Volume 8, Issue 7, 1817-1828. Research Article ISSN 2277– 7105

*Corresponding Author

Prof. Boyapati Madhulatha

Assoc. Professor, GBN

Institute of Pharmacy- Gbn

Institute of Pharmacy

Affiliated to Jawaharlal

Nehru Technological

University, Hyderabad,

Telangana, 501301.

Article Received on

11 April 2019,

Revised on 02 May 2019,

Accepted on 22 May 2019

DOI: 10.20959/wjpr20197-15129



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common hair problem in cosmetics and also in Primary Health Care Practice, has been

recognized for more than 2000 years. Approximately 0.2%-2% of the world population has

been affected from alopecia.[3]

As the World depending on the herbal formulations rather thancosmetic preparations for

beautifying their hair we have made attempt to solve the issues related to hair disorders and

make hair thicker and stronger by formulating a herbal preparation using plant ingredients

fresh as well as dried till date there is no evidence of attempt made to prepare such a

formulations using these plant ingredients. [Amla, Hibiscus, Bringaraj, Bramhi, Henna,

Terminalia Belerica, Flaxseeds, Oats, Calendula, Neem, Tridax, And Tulasi.].

MATERIALS AND METHODS

Collection of Plant Parts

Various plant material where collected that is amla, hibiscus, bringaraj, bahmi,

termineliabelarica, flaxseeds, oats, calendula, neem, tridax, tulsi from in and around

korremula region and were properly authenticated in the department of pharmacognosy., Gbn

Institute of Pharmacy, Hyderabad, Telangana.

Formulation of Herbal Hair Oil: The hair oil was prepared by two different methods.

Paste Method[4]

: All the fresh ingredients were accurately weighed and where converted into

a Paste with very little amount of water and kept overnight after this the wetted drug was

mixed in a mixture of coconut oil and castor oil and boiled with continuous stirring at

constant temperature until the water droplets in the oil stop knocking and the drug has

completely extracted in the oil was then filter through muslin cloth. Three formulations were

prepared using paste method. The quantities of the ingredients taken in different formulations

are as follows.

Table No. 1: Formulations - Paste Method.

Ingredients F1 F2 F3

Amla 75gm 50gm 25gm

Hibiscus 75gm 50gm 25gm

Bringaraj 75gm 25gm 75gm

Brahmi 50gm 25gm 75gm

Henna 25gm 75gm 50gm

TermineliaBelanica 25gm 75gm 50gm

Flax Seeds 25gm 25gm 25gm

Oats 25gm 25gm 25gm



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Calendala 25gm 25gm 25gm

Neem 10gm 10gm 10gm

Tridax 10gm 10gm 10gm

Tulsi 25gm 25gm 25gm

Castor oil 200ml 200ml 200ml

Coconut Oil 800ml 800ml 800ml

Jasmin Oil q.s q.s q.s

Csmphore q.s q.s q.s

Muslin Cloth Method[4]

All the fresh ingredients which are dried and accurately weighed and are converted intodried

powder then tied in a muslin cloth. This cloth was then hanged in a mixture of coconut oil

and castor oil with continuous boiling stirring and finally the oil was filtered. Three

formulations were prepared using muslin cloth method. The quantities of the ingredients

taken in different formulations and where as follows.

Table No. 2: Formulations - Muslin Cloth Method.

Ingredients F1 F2 F3

Amla 100gm 150gm 75gm

Hibiscus 50gm 75gm 100gm

Bringaraj 75gm 50gm 100gm

Brahmi 100gm 150gm 50gm

Henna 75gm 50gm 100gm

TermineliaBelanica 50gm 75gm 100gm

Flax Seeds 50gm 50gm 50gm

Oats 25gm 25gm 25gm

Calendala 25gm 25gm 25gm

Neem 10gm 10gm 10gm

Tridax 10gm 10gm 10gm

Tulsi 25gm 25gm 25gm

Castor oil 200ml 200ml 200ml

Coconut Oil 800ml 800ml 800ml

Jasmin Oil q.s q.s q.s

Csmphore q.s q.s q.s

Fig. 1: Ingredients-Paste method. Fig. 2: preparation by paste method.



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Fig 3: Ingredients-Cloth pouch. Fig 4: preparation by Cloth pouchmethod Method.

Physical Parameters[5, 6, 7]

Sensitivity Test: The prepared herbal hair oil was applied on 1cm skin of hand and exposed

to sunlight for 4-5 hrs.

PH

: 1ml of the oil was weighed in a test tube. 9ml of water was added. PH of the mixture was

determined with the help of pH

meter.

Specific Gravity Test: The specific gravity of the oils were calculated from the following

relationship Specific gravity at 30ᵒc =a-b /c Where, a=weight of specific gravity bottle with

oil at 300c (g); b=weight of specific gravity bottle at 30

oc (g); c=weight of specific gravity

bottle with water at 30oc (g).

ACID Value: Preparation of 0.1 Molar solution-weighed 0.56 g KOH pellets and dissolved

in 100 ml of distilled water and stirred continuously. The prepared 0.1 molar KOH solution

was filled in the burette. Preparation of sample measured 10ml oil and dissolved in 25ml of

ethanol and 25ml of ether mixture and shacked. Added 1ml phenolphthalein solution and

titrated with 0.1 Molar KOH solution

Acid value=5.16V N/W

Saponification Value

Accurately weighed 1ml of oil into a 250 ml of conical flask and 10ml of ethanol ether

mixture (2:1) Was added. To this flask 25ml of 0.5 N alcoholic KOH was kept the flask for

30 min. Add the flask was cooled. The cooled solution was titrated against 0.5 N HCL using

phenopthaleneindicators. Similarly the blank titration was performed without taking oil.

Amount of KOH in mg used was calculated

Saponification value =28.05(B-S)



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Biological Evaluation[8]

Primary Skin Irritation Test

The prepared formulations were assessed for primary skin irritation test. six healthy rats were

selected for the study. Each rat was caged individually food and water given during the test

period 24 hrs. Period to the test. The hair from the back of each rat of 1cm2

was shaved on the

side of the spine to expose sufficiently large test areas which could accommodate three testy

sites were cleaned with surgical spirit. measured quantity (1ml)(5%w/w) of the formulations

ODI,OD2 and OD3 were applied over the respective test sites on one side of the spine and

observed for erythema and edema for 48hrs after application.

Hair Growth Initiation Test

A quantitative model by Uno was used with slight modification for the study of hair growth

initiation. The rabbits were divided in 4 groups 1 rabbit each and 2 cm2

areas were shaved to

remove hairs. Eleven patches were developed on each rabbit. Rabbit of group A was treated

with amla oil of 1-10% concentation on individual patches keeping first patch as control.

Similarly rabbits of other three groups were subjected to brahmi, methiand hibiscus oils

respectively with different concentrations in same pattern. This treatment was continued for

15 days and during the course the hair growth initiation pattern was observed and reported.

Ethical Issues[9]

All the animals were handled in accordance with the internationally accepted principles and

guidelines for the care and use laboratory animals in 2010[26]

and approved by the college

ethical committee.

RESULTS AND DISCUSSION

The formulated herbal hair oils where subjected to physical and biological evaluations.

Physical Evaluation Parameters

Appearance

Paste Method and Muslin Cloth Method

The formulations prepared by paste method PF1, PF2, PF3 were Olive green colour, Light

olive green colour and Dark green colour respectively. And muslin cloth method DMF1,

DMF2, DMF3 were Dark greenish black, Greenish black colour, Light greenish colour.



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Table No. 3: Appearance of Different Formulated Oils.

S. No Formulation Appearance

1 PF1 Olive green colour

2 PF2 Light olive green colour

3 PF3 Dark green colour

4 DMF1 Dark greenish black

5 DMF2 Greenish black colour

6 DMF3 Light greenish colour

Table No: 4 pH OF Different Formulated Oils.

S. No Formulation pH

1 PF1 6.2

2 PF2 6

3 PF3 5.9

4 DMF1 6.2

5 DMF2 6.4

6 DMF3 6.5

Table No. 5: Sensitivity Test of Different Formulated Oils.

S. No Formulation Sensitivity

1 PF1 No Irritation

2 PF2 No Irritation

3 PF3 No Irritation

4 DMF1 No Irritation



Table No. 6: Specific Grvity of Different Formulated Oils.

S. No Formulation Specific Gravity

1 PF1 0.193

2 PF2 0.86

3 PF3 0.96

4 DMF1 0.93

5 DMF2 0.95

6 DMF3 0.940

Table No. 7: Acid Value of Different Formulated Oils.

S. NO Formulation Acid Value

1 PF1 3.35

2 PF2 2.25

3 PF3 2.24

4 DMF1 2

5 DMF2 1.5

6 DMF3 1.5



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Table No 8: Saponification Value of Different Formulated Oils.

S. No Formulation Saponificationvalue

1 PF1 250

2 PF2 248

3 PF3 240

4 DMF1 205

5 DMF2 190

6 DMF3 195

Table No. 9: Evaluation of Hair Oil of Different Formulated Oils.

S. No. Formulation No. of

Rabbits

Time Taken to Intite

Growth (in Days )

Time Taken to Complete

Growt (in Days)

1 Control 1 18 40

2 Standard 1 10 28

3 PF1 1 14 35

4 PF2 1 13 34

5 PF3 1 13 35

6 DMF1 1 12 29

7 DMF2 1 11 28

8 DMF3 1 10 27

Fig. 5: Graphical Representation of pH, Specific Gravity, Acid Value.

Fig. 6: Graphical Representation of Saponification Value.



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The results showed that the formulations indicated no erythema or edema after skin test. This

indicates that the prepared formulations were non-irritant on skin of rabbits. The pH

was

almost near to neutral as given in the table no (4). The specific gravities of the formulations

are given in the table no (6). Acid value is an indication of rancid sate. Lower the acid value

higher the quality of oil as given in the table no (7). Saponification values are highly

significant in the making of soap. It is important that the saponification values is just right too

high and the soap might contain too much alkali even through three is sufficient soapiness

that it would react with skin whilst saponification value too small the fatty acid salts will not

be sufficient enough to remove or saponify the fat or oil and less soapiness values are given

in the table no (8).

A plethora of herbs have been employed for hair treatments. A few of these are amla,

hibiscus, bringraj, brahmi, henna, termineliabelerica flaxseeds, oats, calendula, neem, tridax,

tulsi.[10,11]

Synthetic drug, minoxidil is a potent vasodilator appears safe for term treatment.

After five years use of 2 and 3% topical minoxidil, the improvement has been shown to peak

at one year with a slow decline in regrowth over subsequent years.[12]

Long term treatment

with local side effects may be a problem with continuing used of minoxidillotion.[13]

On the

basis of market survey carried out oil. Hence the presence study was aimed to evaluate the

hair growth activity of herbal formulations which includes oil extract of all mentioned drugs

in various concentrations. In order to justify the traditional now a days multi ingredient hairs

oils reprepared And tested for their hair growth activity.

Amla is rich in vitamin C and minerals such as phosphorus, iron and calcium which provides

nutrition to hair and also causes darkening of hair.[14]

Hibiscus consists of calcium,

phosphorus, iron, vitamin B, riboflavin, niacin, and vitamin C, used to stimulated thicker hair

growth and prevents premature greying of hair.[15]

Brahmi contains alkaloids which enhance

protein kinase activity[16]

methi contains high protein fodderwhich supply required protein

nutrition to hair.[17]

The hair growth initiations were started on 11th

and 10th

day for DMF2,

DMF3 and were comparable to theminoxidil results given in the table no 9. Hence the hair

growth initiation was faster in the formulations DMF2, DMF3 respectively as given in the

table no 9.

Growth completion hair growths was completed on 28th

, 27th

day for DMF2 and DMF3 and

were comparable to theminoxidil results given in the table no 9. On overall basics of physical



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parameters as well as physiological evaluation DMF3 followed by DMF2 formulations were

found more significant and were comparable that of Marketed synthetic drug minoxidil.

Control

After Shavegrowth Initiation Day -18growt Complete Day-40

Standard

After Shavegrowth Initiation Day- 10growth Complete Day-28.

PF1

After Shave Growth Initiation Day- 14 Growth Comlete Day – 35



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PF2

After Shave Growth Initiation Day -13growt Complete Day-34.

PF3

After Shave Growth Initiation Day- 13growt Complete Day-25

DMF1

Afeter Shave Growth Initiation Day-12 Growth Complete Day-29

DMF2

After Shavegrowth Initiation Day - 11growth Coplete Day-28



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DMF3

After Shavegrowth Initiation Day – 10 Growth Complete Day-27.

Fig. 7: Pictorial Representation of Hair Growth After Applying Different Formulated

Oils on Rabbits.

CONCLUSION

This study demonstrated that the various herbal hair oil formulations prepared. Have

promising hair growth activity and provides guideline on the uses of herbal ingredients on the

preparation of herbal hair oil having minimal or no side effects. Thus the formulation was

superior to the marketed brands due to its non-irritancy, safety and complete absence of any

artificial ingredients. It holds the promise of potent herbal alternative for Minoxidil. Also

suggest excellent results of hair growth formula prepared by cloth pouch boiling method.

Further work needs to be carried out in order to establish the Mechanism of Action.

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baldness. Indian drugs, 2002; 39(1): 567-573.

4. Md. Shahinoor Rahaman Dulal, Hasib Sheikh, Mohammad Abu Taher, Mohammad

Sayeed Ur Rahaman, Zakia Rahaman & M.A. Malek. Formulation and finding out the

Efficacy of the Herbal Hair Oil over simple coconut oil (purified) International Journal of

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