Mechanical Characterization of Cellulose Nanofiber and Bio-based Epoxy Composite
Preparation and characterization cellulose draft final final
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Transcript of Preparation and characterization cellulose draft final final
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Preparation and Characterization of Cellulose
Fibers for Biochemical Applications
Delaine Zayaz-Bazán1, José A. Cruz-Arzón1, Nelson Granda-Paz1, and Wilfredo Otaño-Rivera3,4
1-Department of Biology, University of Puerto Rico at Cayey, Puerto Rico1-Department of Chemistry, University of Puerto Rico at Mayagüez, Puerto Rico
3- Department of Physics-Mathematics, University of Puerto Rico at Cayey, Puerto Rico
4-Institute for Functional Nanomaterials, University of Puerto Rico, San Juan, Puerto Rico
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Problem•Is it possible to prepare electrospun
cellulose fibers that could be used for separation of proteins producing high flow and mass transfer?
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Objectives• Prepare cellulose acetate fibers by
electrospinning and transform them into cellulose by a deacetylation process
• Characterize fibers by SEM and FTIR among other techniques.
• Evaluate chemical and physical electrospinning process parameters in order to craft porosity of cellulose fibers.
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Basic ConceptsCellulose Acetate Cellulose
• Molar Mass 30,000
• Density 1.3 g/cm3
• Molar Mass 600,000
• Density 1.5 g/cm3
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•Cellulose Acetate for fiber synthesis
•Cellulose for protein separation
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Methodology
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Methodology
+
Cellulose acetate Acetone/DMF/EtOH
Cellulose 21% sol
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Electrospinning
• Typical conditions▫ Distance: 18 cm▫ 20.0 kV▫ 0.5 mL/ hr
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Results
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21% CA fibers
Results
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Fiber Diameter
Average 693 ±306
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Deacetylation
Regenerated Cellulose Membrane
OH-
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500100015002000250030003500400045005000
0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
1
AC.jws.dx
Wavenumbers [1/cm]
Tran
smitt
ance
1745
FTIR Analysis
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1740
FTIR Analysis
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Deacetylation
•Cellulose Acetate
•Cellulose
Esther Functional Group
Hydroxyl Group
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0500100015002000250030003500400045005000
-0.02
0
0.02
0.04
0.06
0.08
0.1
0.12
0.14
control #2 C Vibha Smooth.jws.dx smoothed smoothedm#D CR.jws.dx smoothed smoothed
Wavenumbers [1/cm]
Tra
nsm
itta
nce
1640
FTIR Analysis
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Regenerated vs. Comercial Cellulose
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Porosity Measurements
±9
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Conclusions
•The work is still going on ▫The preliminary results show that cellulose
can be regenerated from the acetate•Future Works
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Acknowledgements
•UPR at Cayey
•RISE Program
•NAMAS Lab
•Our Lab Group
•Professor Vibha Bansal
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Thank You
Questions?