Pre-Final Draft 3 Phosphate Prouct Sheet
2
334 Hecla Street, Lake Linden, MI 49945 [email protected] | 1.906.296.1130 [email protected] | 1.906.296.1115 www.nitrate.com Specificity: Sensitivity: Analysis Ranges: Sample Types: Storage Conditions: Active Temperature Range: Buffer Specifications: Enzymatic Orthophosphate Test NECi’s Enzymatic Orthophosphate Test is a highly specific, sensitive and selective assay for the quantitative analysis of soluble reactive phosphate (SRP) in many types of sample matrices. Quantitative orthophosphate analysis is useful for evaluating soil health, environmental health, industry process control, wastewater nutrient levels, and much more. This method is compatible with the persulfate digestion for total phosphorus analysis. Total P kits based on the USGS method will be available soon. Utilizes a recombinant enzyme Purine nucleoside phosphorylase (PNP) to catalyze the reaction of phosphate with substrate 2-amino-6-mercapto-7-methylpurine ribonucleoside (MESG). The resulting 2-amino-6-mercapto-7-methylpurine (AMMP) absorbs at 360 nm for accurate photometric analysis of phosphate. Replaces the ascorbic acid method for more precise analysis. Results in phosphorus units are obtainable with a simple conversion factor. Small reaction volume, minimal sample preparation, & non-hazardous materials or waste. Available in test tube, microplate, & on-site formats or build your own assay with enzyme reagent packs. Orthophosphate 15 - 40 °C 200 mM HEPES | 20 mM MgCl 2 pH 7.6 Water Soil Aqueous Extracts Low: 0.5 - 5.0 ppm Standard: 5.0 - 20.0 ppm 0.5 ppm MDL 3+ Years @ -80°C 1+ Year @ -20°C 6 Months @ 4°C
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Transcript of Pre-Final Draft 3 Phosphate Prouct Sheet
334 Hecla Street, Lake Linden, MI [email protected] | [email protected] | 1.906.296.1115www.nitrate.com
Specificity:
Sensitivity:
Analysis Ranges:
Sample Types:
Storage Conditions:
Active Temperature Range:
Buffer Specifications:
Enzymatic Orthophosphate Test
NECi’s Enzymatic Orthophosphate Test is a highly specific, sensitive and selective assay for the quantitative analysis of soluble reactive phosphate (SRP) in many types of sample matrices.
Quantitative orthophosphate analysis is useful for evaluating soil health, environmental health, industry process control, wastewater nutrient levels, and much more.
This method is compatible with the persulfate digestion for total phosphorus analysis. Total P kits based on the USGS method will be available soon.
Utilizes a recombinant enzyme Purine nucleoside phosphorylase (PNP) to catalyze the reaction of phosphate with substrate 2-amino-6-mercapto-7-methylpurine ribonucleoside (MESG). The resulting 2-amino-6-mercapto-7-methylpurine (AMMP) absorbs at 360 nm for accurate photometric analysis of phosphate.
Replaces the ascorbic acid method for more precise analysis.
Results in phosphorus units are obtainable with a simple conversion factor.
Small reaction volume, minimal sample preparation, & non-hazardous materials or waste.
Available in test tube, microplate, & on-site formats or build your own assay with enzyme reagent packs.
Orthophosphate
15 - 40 °C
200 mM HEPES | 20 mM MgCl2pH 7.6
WaterSoilAqueous Extracts
Low: 0.5 - 5.0 ppmStandard: 5.0 - 20.0 ppm
0.5 ppm MDL
3+ Years @ -80°C1+ Year @ -20°C6 Months @ 4°C
334 Hecla Street, Lake Linden, MI [email protected] | [email protected] | 1.906.296.1115
A Closer Look at the Reaction
Overview of the Method
Example Standard Curve
H2PO4-
2-amino-6-mercapto-7-methylpurine ribonucleoside
+
2-amino-6-mercapto-7-methylpurine
Purine Nucleoside Phosphorylase
MESG
Ribose-1-phosphate
+
Wait10 Min
900 µLReaction Mixture
Combine Reaction Mixture + Sample into test tube
100 µLSample
Fill to 20.5 mLwith Bu�er
Prepare Stock Reaction Mixture
1 mL PNP
1 mL MESG
By using a set of phosphate standards of known concentration, a standard curve is used to calibrate the method and quantify phosphate content of unknown samples.
When used in conjunction with NECi’s Handheld Photometer, results can be obtained on any Android mobile device. The app software has a pre-programmed standard curve and displays data in ppm phosphate.
Measure A-360 of each sample in UV compatible cuvettes
Note: ppm Phosphate x 0.3262 = ppm Phosphorus
Order online at nitrate.com/store/index.php/phosphate
