7/30/2019 Practical Microbiology 1 - Culture for Gonorrhoeae, Syphilis, Chlamydia and Mycoplasma

1/8

MODULE 16 THE GENITAL SYSTEM (MICROBIOLOGY & IMMUNOLOGY)

PRACTICAL MICROBIOLOGY 1: CULTURE FOR GONORRHOEAE, SYPHILIS,

CHLAMYDIA AND MYCOPLASMA

A)Pictures:-1-Neisseria Gonorrhoeae

Gram negative

diplococci

Neisseria smear

gram stain

7/30/2019 Practical Microbiology 1 - Culture for Gonorrhoeae, Syphilis, Chlamydia and Mycoplasma

2/8

MODULE 16 THE GENITAL SYSTEM (MICROBIOLOGY & IMMUNOLOGY)

2-Treponema Pallidum

Spirochetes by dark

ground microscope

RPR

7/30/2019 Practical Microbiology 1 - Culture for Gonorrhoeae, Syphilis, Chlamydia and Mycoplasma

3/8

MODULE 16 THE GENITAL SYSTEM (MICROBIOLOGY & IMMUNOLOGY)

3-Mycoplasma

Fried egg

appearance

Mycoplasma EM

7/30/2019 Practical Microbiology 1 - Culture for Gonorrhoeae, Syphilis, Chlamydia and Mycoplasma

4/8

MODULE 16 THE GENITAL SYSTEM (MICROBIOLOGY & IMMUNOLOGY)

4-Chlamydia

Chlamydia inclusionbodies by Geimsa

Stain

7/30/2019 Practical Microbiology 1 - Culture for Gonorrhoeae, Syphilis, Chlamydia and Mycoplasma

5/8

MODULE 16 THE GENITAL SYSTEM (MICROBIOLOGY & IMMUNOLOGY)

B)Culture:-

Chocolate

agar

Oxidase test

positive

7/30/2019 Practical Microbiology 1 - Culture for Gonorrhoeae, Syphilis, Chlamydia and Mycoplasma

6/8

MODULE 16 THE GENITAL SYSTEM (MICROBIOLOGY & IMMUNOLOGY)

C)Slides:-

Neisseria gonorrhoeae

*Smear with gram stain showing diplococci inside and outside

polymorphs

7/30/2019 Practical Microbiology 1 - Culture for Gonorrhoeae, Syphilis, Chlamydia and Mycoplasma

7/8

MODULE 16 THE GENITAL SYSTEM (MICROBIOLOGY & IMMUNOLOGY)

Neisseria gonorrhoeae

*Smear with methelene blue stain showing diplococci inside and

outside polymorphs.

7/30/2019 Practical Microbiology 1 - Culture for Gonorrhoeae, Syphilis, Chlamydia and Mycoplasma

8/8

MODULE 16 THE GENITAL SYSTEM (MICROBIOLOGY & IMMUNOLOGY)

Neisseria gonorrhoeae from culture

*Gram negative diplococci