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NAME :

CLASS :

SMK ST XAVIER, PULAU PINANG

PEPERIKSAAN PERTENGAHAN TAHUN 2013

BIOLOGYPaper 3May1 ½ hours One hour and thirty minutes ____________________________________________________________________________ 

For examiner use only 

Question Full marks Marks

1 33

2 17

Total 50

INFORMATION FOR CANDIDATES

1. This question paper consists of twoquestions: Question 1 and Question 2. 

2. Answer  all questions. Write your answer for Question 1 in the spaces provided inthis question paper.

3. Write your answer for Question 2 in thespaces provided at page 7 and 8. You may

use equations, diagrams, tables, graphsand other suitable methods to explain your answer.

4. The diagrams in the questions are notdrawn to scale unless stated.

5. You are advised to spend 45 minutes toanswer Question 1 and 45 minutes for Question 2.

6. You may use a non-programmablescientific calculator.

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1. A group of students carried out an experiment to study the anaerobic respiration in yeast.Diagram 1.1 shows the set-up of the experiment.

The thermometer readings and the observations on the lime water were taken at 0 minute and30 minutes. Diagram 1.2 shows the observations made on the lime water.

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Table 1 shows the result of the experiment at 0 minute and 30 minutes.

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(a) State the thermometer readings in the spaces provided in Table 1. [3 marks]

(b) (i) Based on Table 1, state two observations that can be made in this experiment.

Observation 1 :

 ______________________________________________________________________  ______________________________________________________________________ 

Observation 2 :

 ______________________________________________________________________ 

 ______________________________________________________________________ 

[3 marks]

(ii) State the inferences from the observations in 1(b)(i).

Inference from observation 1:

 ______________________________________________________________________ 

 ______________________________________________________________________ 

Inference from observation 2 :

 ______________________________________________________________________ 

 ______________________________________________________________________ 

[3 marks]

(c) Complete Table 2 based on this experiment.

Variable Method to handle the variable

Manipulated variable

 ____________________________ 

 ____________________________ 

 ___________________________________________ 

 ___________________________________________ 

Responding variable

 ____________________________ 

 ____________________________ 

 ___________________________________________ 

 ___________________________________________ 

Constant variable

 ____________________________ 

 ____________________________ 

 ___________________________________________ 

 ___________________________________________ 

[3 marks]

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(d) State the hypothesis for this experiment.

 _________________________________________________________________________ 

 _________________________________________________________________________ 

 _________________________________________________________________________ 

[3 marks]

(e) (i) Construct a table and record all the data collected in this experiment.Your table should have the following titles:

Set / Presence of yeast

Thermometer reading

Change in lime water 

[3 marks]

(ii) Explain the relationship between the boiled glucose solution and paraffin oil used in theexperiment of the anaerobic respiration process in yeast.

 _______________________________________________________________________ 

 _______________________________________________________________________ 

 _______________________________________________________________________ 

 _______________________________________________________________________ 

[3 marks]

(f) Following are some of the apparatus and materials used in the experiment.

Categorise the apparatus and materials according to their roles in operating the variables inthe experiment.

Yeast suspension, boiled glucose solution, distilled water, thermometers, lime water and

paraffin oil

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[3 marks]

(g) Based on the experiment, define operationally anaerobic operation.

 _________________________________________________________________________ 

 _________________________________________________________________________ 

 _________________________________________________________________________ 

 _________________________________________________________________________ 

[3 marks]

(h) The experiment is repeated by using set C containing 10 cm3 of yeast suspension and10cm

3of boiled glucose solution.

(i) Predict the reading on the thermometer used in the experiment. Explain your answer.

 _______________________________________________________________________ 

 ________________________________________________________________________ 

 ________________________________________________________________________ 

 ________________________________________________________________________ 

[3 marks]

(ii) Explain the time taken for the lime water to change.

 ________________________________________________________________________ 

 ________________________________________________________________________ 

 ________________________________________________________________________ 

 ________________________________________________________________________ 

[3 marks]

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2. Elaine made fruit pickles using unripe mango. During preparation, she placed the mangoslices in water and later placed them in sugar solution. When the mango slices were inwater, it was found that, the slices became turgid and their sizes increased. But when theywere placed in the sugar solution, the slices became soft and shrunken.

Based on the above situation, plan a laboratory experiment to determine the concentrationof sucrose which is isotonic to the cell sap of mango. The planning of your experiment mustinclude the following aspects:

Problem statement

Aim of investigation

Hypothesis

Variables

List of apparatus and materials

Technique used Experimental procedure and method

Presentation of data

Conclusion[17 marks]

 ____________________________________________________________________________ 

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END OF QUESTION PAPER