3D VS 2D

In 3D cell culture, cells attach to one another and form natural cell-to-cell attachments.

These gap junctions are much more prevalent in 3D cell culture than 2D and these junctions enable cells to communicate with each other via exchange of ions, small molecules and even electrical currents.

3D culture have more tissue differentiation, cell adherence property.

Cell grown in 3D culture could not be killed by low doses of chemotherapeutic drug or low doses of radiation.

In 2D culture cell does not attached properly.

Gap junction are not much widespread in 2D culture.

Less tissue differentiation

cell could be easily killed by low doses of drug.

LAB ON CHIP

A lab-on-a-chip is a device that integrates one or several laboratory functions on a single chip that deals with handling particles in hollow microfluidic channels. Advantages

Lowering fluid volume consumption (lower reagents costs, less waste), increasing portability of the devices, increasing process control (due to quicker thermo-chemical reactions) and decreasing fabrication costs.