Tumor Necrosis Factor Alpha Production from CD8 T Cells Mediates ...
Pp65-specific all B: CD8 T-cells CD8/pp65 T cells per ml blood A: CD8 T-cells Indicated memory...
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pp65-specific all B: CD8 T-cells CD8/pp65 T cells per ml blood A: CD8 T-cells Indicated memory subset as a proportion of the reference T- cell subset FIG. S1. The size of pp65-specific responses determines the memory T- cell subset distribution within that response but also within the entire T-cell compartment. Scatter plots show the correlation of CD8/pp65 absolute response sizes (cells per ml of blood) versus the memory subset distribution within these responses (A) or in the entire CD8 T-cell compartments (B) in 39 donors. Significant correlations with R S >0.50 are highlighted bold. Counts are shown on a linear scale for better visualization of subtle changes. R S =-.387, p=.015 R S =.378, p=.018 R S =.026, ns R S =.010, ns R S =.318 p=.049 R S =.030, ns R S =-.215, ns R S =-.610, p=.000 T Naive T EMRA T CM T EM
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Transcript of Pp65-specific all B: CD8 T-cells CD8/pp65 T cells per ml blood A: CD8 T-cells Indicated memory...
pp65-specific allB: CD8 T-cells
CD8/pp65 T cells per ml blood
A: CD8 T-cellsIn
dic
ated
mem
ory
su
bse
t a
s a
pro
po
rtio
n o
f th
e re
fere
nce
T-c
ell
sub
set
FIG. S1. The size of pp65-specific responses determines the memory T-cell subset distribution within that response but also within the entire T-cell compartment. Scatter plots show the correlation of CD8/pp65 absolute response sizes (cells per ml of blood) versus the memory subset distribution within these responses (A) or in the entire CD8 T-cell compartments (B) in 39 donors. Significant correlations with RS>0.50
are highlighted bold. Counts are shown on a linear scale for better visualization of subtle changes.
RS =-.387, p=.015
RS =.378, p=.018
RS =.026, ns
RS =.010, ns
RS =.318 p=.049
RS =.030, ns
RS =-.215, ns
RS =-.610, p=.000
TNaive
TEMRA
T CM
TEM
Ind
icat
ed m
emo
ry s
ub
set
as
a p
rop
ort
ion
of
the
refe
ren
ce T
-cel
l su
bse
t
RS =-.302, ns
RS =.440, p=.006
RS =-.142, ns
RS =.304, ns
CD4/pp65 T cells per ml blood
RS =.573 p=.000
RS =-.489, p=.002
RS =-.230, ns
pp65-specific allA: CD4 T-cells B: CD4 T-cells
RS =-.530, p=.001
FIG. S2. The size of pp65-specific responses determines the memory T-cell subset distribution within that response but also within the entire T-cell compartment. Scatter plots show the correlation of CD4/pp65 absolute response sizes (cells per ml of blood) versus the memory subset distribution within these responses (A) or in the entire CD4 T-cell compartments (B) in 38 donors. Significant correlations with RS>0.50
are highlighted bold. Counts are shown on a linear scale for better visualization of subtle changes.
TNaive
TEMRA
T CM
TEM
T cells with 4 activation markers
T cells with 3 activation markers
T cells with 2 activation markers
T cells with 1 activation marker
T cells without activation markers
B
IFN
-g
CD4 T-cells
IFN
-g
A
TNF-a
CD8 T-cells
TNF-a
FIG. S3. Polyfunctional T-cells display larger quantities of each activation marker. Representative dot plots show the expression levels (MFI) of IFN- and TNF- of pp65/CD8 and pp65/CD4 T-cell subsets displaying different numbers of activation markers after stimulation with pp65 for 16 hours. T-cells expressing no (black), one (red), two (blue), three (green) or four (orange) activation markers are shown. Dot plots show log mean fluorescence intensities.
