Pp 295 - 301. 2. Replication Elongation DNA polymerase – enzyme which does what? In what...
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Transcript of Pp 295 - 301. 2. Replication Elongation DNA polymerase – enzyme which does what? In what...
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DNA Replication Elongationpp 295 - 301
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2. Replication ElongationDNA polymerase – enzyme which does what? In what
direction? What is the main version responsible for replication?in prokaryotes: DNA polymerase I, II, III, IV & Vin eukaryotes: over 15 different types
Nucleotide chains only form in the 5’ 3’ direction.
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DNA Polymerase III Substrates1. DNA template2. deoxyribonucleoside triphosphates (dNTPs)
3. RNA primer
5’3’
5’3’
5’ 3’
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2. Replication Elongation1. Primase:
5’3’
5’ 3’
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A
2. Replication ElongationThree steps involved in the addition of every
nucleotide to the new piece of DNA are:
AT
TTP P P
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2. Replication Elongation
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2. Replication ElongationIn what direction must the DNA polymerase produce
the new strand of DNA?
Which direction does the replication fork open?
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2. Replication ElongationProkaryotic DNA is circular. Two DNA polymerases
replicated in opposing directions until both strands are completed.
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2. Replication ElongationEukaryotic DNA is linear. DNA polymerases in travel
both directions from the ori site.
What direction is DNA synthesized?
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Leading Strand
5’
5’
3’
3’
helicase
SSBPs
gyrase
primase
5’
3’
DNA polymerase III
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Lagging Strand
Okazaki fragment
5’
3’
5’
3’
5’
3’
helicasegyrase
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2. Replication ElongationOkazaki fragments:
DNA polymerase I –
DNA ligase –
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2. Replication ElongationDNA replication occurs:
1. continuously on the _______ strand
2. discontinuously on the _______ strand
Replication Animation
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3. Replication TerminationTelomeres
TTAGGG
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Telomeres
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TelomeraseExplain what happens to the telomere during each
round of replication, why this is important and why some cells have a mechanism to repair this while others do not…
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TelomeraseTelomeres and aging?
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TelomeraseTelomerase:
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Mistakes in DNA ReplicationFewer than 1 error in 107 NTs
Exonuclease –
Roles of DNA polymerase I and II
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ClassworkIntroduce yourself to your neighbor
Everyone get into groups of 2-3
In groups, summarize DNA replication as a series of steps