Power Point - Schekman Lecture 3, Membrane Structure Dynamics
-
Upload
summittliu -
Category
Documents
-
view
40 -
download
0
description
Transcript of Power Point - Schekman Lecture 3, Membrane Structure Dynamics
![Page 1: Power Point - Schekman Lecture 3, Membrane Structure Dynamics](https://reader036.fdocuments.us/reader036/viewer/2022082821/55cf9757550346d03391188e/html5/thumbnails/1.jpg)
Schekman Lecture 3
Membrane Structure: Dynamics
T3
Whitney Smith, Geneth Chin, Summitt Liu
![Page 2: Power Point - Schekman Lecture 3, Membrane Structure Dynamics](https://reader036.fdocuments.us/reader036/viewer/2022082821/55cf9757550346d03391188e/html5/thumbnails/2.jpg)
Two Protein LinkagesAsparagine bonded to Acetylglucosamine Serine bonded to Acetylglucosamine
![Page 3: Power Point - Schekman Lecture 3, Membrane Structure Dynamics](https://reader036.fdocuments.us/reader036/viewer/2022082821/55cf9757550346d03391188e/html5/thumbnails/3.jpg)
Fixed Membrane ProteinsGold particles attached to ConA which attaches to Mannose which binds to proteins.
Membrane
Binding to the gold allows the position of the proteins to be seen using electron microscopy.
http://en.wikiversity.org/wiki/File:Gold_molecule-membrane.jpg
![Page 4: Power Point - Schekman Lecture 3, Membrane Structure Dynamics](https://reader036.fdocuments.us/reader036/viewer/2022082821/55cf9757550346d03391188e/html5/thumbnails/4.jpg)
Protein Placement on Cell Membrane
1) Peripheral Protein2) Peripheral Protein3) Protein Spans across bilayer4) Integral Protein5) Peripheral Protein (to protein 4)
http://en.wikiversity.org/wiki/File:Cell_membrane_protein_diagram.jpg
![Page 5: Power Point - Schekman Lecture 3, Membrane Structure Dynamics](https://reader036.fdocuments.us/reader036/viewer/2022082821/55cf9757550346d03391188e/html5/thumbnails/5.jpg)
SDS Gel Electrophoresis Band of larger proteins
http://en.wikipedia.org/wiki/File:SDS-PAGE.jpg
Bands of smaller proteins
![Page 6: Power Point - Schekman Lecture 3, Membrane Structure Dynamics](https://reader036.fdocuments.us/reader036/viewer/2022082821/55cf9757550346d03391188e/html5/thumbnails/6.jpg)
Immunoblotting (Western Blot Technique)
Proteins transfer onto filter paper at the bottom of the gel
https://ww2.chemistry.gatech.edu/~lw26/bCourse_Information/4581/techniques/gel_elect/gel.jpg
![Page 7: Power Point - Schekman Lecture 3, Membrane Structure Dynamics](https://reader036.fdocuments.us/reader036/viewer/2022082821/55cf9757550346d03391188e/html5/thumbnails/7.jpg)
Nitrocellulose Filter Paper
http://en.wikiversity.org/wiki/File:SDS-PAGE_Coomasie_stained.jpg
![Page 8: Power Point - Schekman Lecture 3, Membrane Structure Dynamics](https://reader036.fdocuments.us/reader036/viewer/2022082821/55cf9757550346d03391188e/html5/thumbnails/8.jpg)
Hydropathy Plot for Glycophorin
http://cc.scu.edu.cn/G2S/eWebEditor/uploadfile/20120810131402735.jpg
Glycophorin
Hydropathy Plot used to gain information about structure of protein
![Page 9: Power Point - Schekman Lecture 3, Membrane Structure Dynamics](https://reader036.fdocuments.us/reader036/viewer/2022082821/55cf9757550346d03391188e/html5/thumbnails/9.jpg)
Bacterial Photoreaction Center Crystal structure for photoreaction center
http://en.wikipedia.org/wiki/File:First_satge_sbep2.svg
http://en.wikipedia.org/wiki/File:Reactprotdatbnk.JPG
Photoreaction Center Diagram