Poultry Vaccinology Summit April 28-30, Budapest, Hungary ... · PDF fileND vaccination...

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Vectormune ND

a step towards control of Newcastle Disease

Yannick Gardin1, Vilmos Palya1, John Elattrache1, Marcelo Paniago2, Christophe Cazaban2,

Fernando Lozano2, Pascal Paulet3

1Scientific and Innovation Department, 2Global Veterinary Services Poultry department, 3Poultry Franchise department.

Poultry Vaccinology Summit

April 28-30, Budapest, Hungary

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For the control of Newcastle Disease (ND)

vaccination is a very efficacious tool,

if complementary to:

The parameters of ND vaccination

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For the control of Newcastle Disease (ND)

vaccination is a very efficacious tool,

if complementary to:


Good animal husbandry

Stringent Biosecurity

Effective Sanitary Police

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ND vaccination program

must match the field disease pressure:


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ND vaccination program

must match the field disease pressure:

Low ND is a potential risk

Medium ND is a real risk

High ND is a strong risk


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Reliable vaccination against ND

must take into account:


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Reliable vaccination against ND

must take into account:

Importance of vaccine administration

including flock coverage

Interference with Passive immunity

(Maternally Derived Antibodies or

MDA)


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The critical points regarding

vaccine immunity against ND are:


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The critical points regarding

vaccine immunity against ND are:

Onset of Immunity (OOI)

Level of Immunity (LOI)

Duration of Immunity (DOI)


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As of today, there are 2+1 categories

of vaccines against ND:

ND vaccines

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Live attenuated

Inactivated (killed) adjuvanted

ND vaccines

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Live attenuated

Inactivated (killed) adjuvanted

rHVT-F (Vectormune ND)

ND vaccines

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The classical ND vaccines

Category + -

Live Fast OOI Local (& general Imm.)

Susceptible to MDA Post Vaccination Reactions Short lasting Immunity

Killed Long lasting Immunity Susceptible to MDA

Local reaction General Immunity only

ND vaccines

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Category + -

Live Fast OOI Local (& general Imm.)

Susceptible to MDA Post Vaccination Reactions Short lasting Immunity

Killed Long lasting Immunity Susceptible to MDA

Local reaction General Immunity only

ND vaccines

The classical ND vaccines

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The problem of interference with MDA

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27 34 41

Age (days)

serum

tears

Antibody titre*

(10 log)

<1

1

2

3

4

5

1 13 21

*ImmunoPeroxydase Monolayer Assay

6


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Live attenuated ND vaccines


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% protection / challenge

0

10

20

30

40

50

60

70

80

90

100

0 5 10 15 20 25 30

Chickens

Control

MDA

with without

Age (days)

(after Bennejean G. et al., 1978)

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0

10

20

30

40

50

60

70

80

90

100

0 5 10 15 20 25 30

Chickens

Vaccinated

Control

MDA

with without

Age (days)


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Chickens

Vaccinated

Control

MDA

with without


0

10

20

30

40

50

60

70

80

90

100

0 5 10 15 20 25 30

Age (days)


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Killed (inactivated) ND vaccines


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Killed (inactivated) ND vaccines



0

20

40

60

80

100

120

0 5 10 15 20 30 40 50 60

Age at challenge (days)

% o

f pro

tect

ion

Killed vaccine - No MDA

Killed vaccine - MDA

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rHVT-F (Vectormune ND) vaccine


YG - 150429 (Extract from study SCI 193-2012 – SSIU Ceva Phylaxia)

La Sota (Phylaxia)

antigen

rHVT-F (Vectormune ND) vaccine


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Efficacy of Vectormune ND

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Laboratory trials in Thailand (vvNDV)


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Laboratory trials in Thailand (vvNDV)


VECTORMUNE HVT-NDV

Laboratory challenge trials in Thailand

0

20

40

60

80

100

120

14 21 28

Age at challenge (days)

Per

cent

age

of p

rote

ctio

n

Vectormune HVT-NDV

Cevac Vitapest L

Cevac Vitapest L + Vectormune HVT-NDV

Cevac Vitapest L + Cevac Broiler ND K

Controls

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Laboratory trials at SSIU Ceva Phylaxia

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- Commercial broilers (20 per group)

- Vectormune ND SQ or in-ovo (-3d)

- Challenge :

- 105 EID50 Chimalhuacan NDV strain

- at 3 or 4 or 6 weeks of age

- IN + ON route

- Oropharyngeal and Cloacal swabs taken 3 and 7 days PC

- Virus quantification using RT-PCR


Laboratory trials at SSIU Ceva Phylaxia

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Clinical protection


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* * * * * * * *

Challenge at D28

Reduction

of

shedding


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* * * * * * * *

Reduction

of

shedding


Challenge at D28

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Does it protect

against

various types

of NDV ?

F

insert


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F

insert Yes !

Does it protect

against

various types

of NDV ?


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F

insert Yes !

Yes !

Does it protect

against

various types

of NDV ?


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F

insert Yes !

Yes !

Yes !

Does it protect

against

various types

of NDV ?


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F

insert Yes !

Yes !

Yes !

Yes !

Does it protect

against

various types

of NDV ?


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- Lohman Brown layer pullets with MDA

- Vectormune ND (d1 – SQ)

- Vectormune ND (d1 – SQ) + Cevac Vitapest L (ED)

+ Cevac ND IB EDS K (w15 – IM)

- Challenge :

- 105 EID50 Malaysian 2010 vvNDV viscerotropic

isolate (Genotype VII) - IN route (0,1 ml)

- at 3, 4, 6, 10, 33, and 72 weeks of age


Controlled trials at SSIU Ceva Phylaxia

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Protection of layers against vvNDV challenge

0

20

40

60

80

100

120

3 4 6 10 15 33

Age at challenge (in weeks)

% P

rote

ctio

n

Vectormune HVT-NDV

Vectormune HVT-NDV +

Cevac Vitapest L + KilledControls

33 72


Controlled trials at SSIU Ceva Phylaxia

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Vectormune ND: vaccination programs

Disease pressure

MDA Vectormune ND Live ND Priming

D1

Live ND boost

D14-18

Low O O

Medium O O O

High O O O O

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Conclusions: Vectormune ND

Vaccine « take » is not prevented by MDA

Vaccine take depends only on the quality of

administration

Administration can be done at the hatchery

(in-ovo or SQ) i.e. in the most reliable way

Early immunity can be re-inforced by MDA

and live vaccine(s) if necessary

Duration of immunity is outstanding

Shedding of challenge virus is reduced

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Conclusions: Vectormune ND

A (major) step towards

a better control of

Newcastle Disease

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THANK YOU

FOR YOUR ATTENTION

Poultry Vaccinology Summit April 28-30, Budapest, Hungary ... · PDF fileND vaccination...

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