Poster 095: Multiple Unerupted Teeth Related to the Under-Expression of MMP-3, MMP-12, and the...
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POSTER 094 The Ameloblastic Fibro-Odontoma in the Mandible: A Case Report and Histopathologic Study Yong-Kwan Choi, Korea (Choi YK; Han SJ; Kim CH; Lee JH; Kim KW) Statement of the Problem: This is a case report of a rare mixed odontogenic tumor, an ameloblastic fibro- odontoma, in the posterior of right mandible. A 2-year- old male patient was referred to our department for a large tumorous lesion on his right mandible. The radiol- ogy examination revealed a large radiopaque and radi- olucent lesion with an impacted and unerupted tooth, #44, #45, #46 #85. After surgical enucleation and curet- tage of the mass, the tumor was confirmed to be an ameloblastic fibro-odontoma. The mass was composed of the dental organs and an island of odontogenic epi- thelium embedded in a cell-rich mesenchymal stroma. An immunohistochemical study was carried out. The results showed positive CK7 staining, and weekly posi- tive staining for Bcl-2 and Ki-67 staining. However, there was no CEA, CK8, CK12 and CK16 staining observed. The follow-up studies over 2 years showed no tumor recurrence. References Gardner DG: The mixed odontogenic tumors. J Oral Surg 58: 166, 1984. Regezi JA, Kerr DA, Caurtney RM: Odontogenic tumors: analysis of 706 cases. J Oral Surg 36:771, 1978. POSTER 095 Multiple Unerupted Teeth Related to the Under-Expression of MMP-3, MMP-12, and the Over-Expression of FGF-5 Seong-Gon Kim, DDS, PhD, Dept. of OMFS, Sacred Heart Hospital, Hallym University, #896 Pyungchon- Dong, Dongan-Gu, Anyang, 431-070, Korea (Kim JW; Kim SG) Statement of the Problem: We report two cases of multiple unerupted teeth and examined their probable molecular pathogenesis using micro-array and quantita- tive RT-PCR. Materials and Methods: Fibroblasts were taken from the patients with multiple unerupted teeth and primary cultured. The total RNA was extracted and examined using a microarray assay. Normal dermal fibroblasts and gingival fibroblasts were used as the control. The results of the microarray were confirmed by quantitative RT- PCR. Method of Data Analysis: n/a Results: The microarray data are as follows. The genes showing up-regulation by more than 2-fold were FGF-5 (fold ratio; 3.721), FGF-11 (fold ratio; 3.085), and BMP-4 (fold ratio; 5.345). The genes shown down-regulation by more than 2-fold were BMP type II receptor (fold ratio; 0.227), Caspase 7 (fold ratio; 0.470), FGF-7 (fold ratio; 0.383), MMP-3 (fold ratio; 0.182), MMP-12 (fold ratio; 0.469), RUNX1 (fold ratio; 0.131). The results of quanti- tative RT-PCR were in accordance with the microarray data. Conclusion: These results suggest that FGF-5 mediates the overgrowth of fibroblasts, and that the down-regula- tion of the MMP-312 activity might be important to the non-eruption of teeth. POSTER 096 A Case of Undifferentiated Carcinoma Arising in the Right Side of the Palate Toshiya Sano, DDS, PhD, 9-1 Motomachi, Suita City, Osaka Fu, 564-0031, Japan (Akamatsu H; Shima M) Statement of the Problem: Undifferentiated carcino- mas relatively rarely develop in the head and neck re- gion, but their prognosis is poor, causing distant metas- tasis in the early stage. We encountered a patient with an undifferentiated carcinoma that developed in the right palate region, and markedly responded to radiotherapy and chemotherapy. Materials and Methods: The patient was a 77-year-old male who visited our department for a chief complaint of swelling of the right palate region on December 26, 2005. Method of Data Analysis: As histopathological find- ings, poorly differentiated atypical cells were noted, and EMA was positive on immunostaining. Based on these findings, undifferentiated cancer was diagnosed. No re- gional lymph node and systemic metastasis was noted on CT, MRI, Ga and bone scintigraphy. Results: As preoperative treatment, radiotherapy at 40 Gy and chemotherapy with one course of TS-1 100 mg/body x 14 days CDDP 10 mg/body x 5 days and one course of TS-1 100 mg/body x 14 days were per- formed. Surgery was planned, but not performed be- cause of the strong request of the patient. Additional irradiation at 30 Gy and chemotherapy with one course of TS-1 100 mg/body x 14 days CDDP 10 mg/body x 5 days markedly reduced the tumor, and the patient was discharged. The patient received 5 courses of chemo- therapy with TS-1 100 mg/body x 14 days at the outpa- tient clinic as of December 2006, and no recurrence was noted. The patient is under course observation, and we will closely follow him. Conclusion: Radiotherapy and chemotherapy were markedly effective for undifferentiated cancer. Scientific Poster Session 43.e56 AAOMS • 2007
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Transcript of Poster 095: Multiple Unerupted Teeth Related to the Under-Expression of MMP-3, MMP-12, and the...
POSTER 094The Ameloblastic Fibro-Odontoma in theMandible: A Case Report andHistopathologic StudyYong-Kwan Choi, Korea (Choi YK; Han SJ; Kim CH;Lee JH; Kim KW)
Statement of the Problem: This is a case report of arare mixed odontogenic tumor, an ameloblastic fibro-odontoma, in the posterior of right mandible. A 2-year-old male patient was referred to our department for alarge tumorous lesion on his right mandible. The radiol-ogy examination revealed a large radiopaque and radi-olucent lesion with an impacted and unerupted tooth,#44, #45, #46 #85. After surgical enucleation and curet-tage of the mass, the tumor was confirmed to be anameloblastic fibro-odontoma. The mass was composedof the dental organs and an island of odontogenic epi-thelium embedded in a cell-rich mesenchymal stroma.An immunohistochemical study was carried out. Theresults showed positive CK7 staining, and weekly posi-tive staining for Bcl-2 and Ki-67 staining. However, therewas no CEA, CK8, CK12 and CK16 staining observed.The follow-up studies over 2 years showed no tumorrecurrence.
References
Gardner DG: The mixed odontogenic tumors. J Oral Surg 58: 166,1984.
Regezi JA, Kerr DA, Caurtney RM: Odontogenic tumors: analysis of706 cases. J Oral Surg 36:771, 1978.
POSTER 095Multiple Unerupted Teeth Related to theUnder-Expression of MMP-3, MMP-12,and the Over-Expression of FGF-5Seong-Gon Kim, DDS, PhD, Dept. of OMFS, SacredHeart Hospital, Hallym University, #896 Pyungchon-Dong, Dongan-Gu, Anyang, 431-070, Korea (Kim JW;Kim SG)
Statement of the Problem: We report two cases ofmultiple unerupted teeth and examined their probablemolecular pathogenesis using micro-array and quantita-tive RT-PCR.
Materials and Methods: Fibroblasts were taken fromthe patients with multiple unerupted teeth and primarycultured. The total RNA was extracted and examinedusing a microarray assay. Normal dermal fibroblasts andgingival fibroblasts were used as the control. The resultsof the microarray were confirmed by quantitative RT-PCR.
Method of Data Analysis: n/aResults: The microarray data are as follows. The genes
showing up-regulation by more than 2-fold were FGF-5(fold ratio; 3.721), FGF-11 (fold ratio; 3.085), and BMP-4(fold ratio; 5.345). The genes shown down-regulation bymore than 2-fold were BMP type II receptor (fold ratio;0.227), Caspase 7 (fold ratio; 0.470), FGF-7 (fold ratio;0.383), MMP-3 (fold ratio; 0.182), MMP-12 (fold ratio;0.469), RUNX1 (fold ratio; 0.131). The results of quanti-tative RT-PCR were in accordance with the microarraydata.
Conclusion: These results suggest that FGF-5 mediatesthe overgrowth of fibroblasts, and that the down-regula-tion of the MMP-3�12 activity might be important to thenon-eruption of teeth.
POSTER 096A Case of Undifferentiated CarcinomaArising in the Right Side of the PalateToshiya Sano, DDS, PhD, 9-1 Motomachi, Suita City,Osaka Fu, 564-0031, Japan (Akamatsu H; Shima M)
Statement of the Problem: Undifferentiated carcino-mas relatively rarely develop in the head and neck re-gion, but their prognosis is poor, causing distant metas-tasis in the early stage. We encountered a patient with anundifferentiated carcinoma that developed in the rightpalate region, and markedly responded to radiotherapyand chemotherapy.
Materials and Methods: The patient was a 77-year-oldmale who visited our department for a chief complaintof swelling of the right palate region on December 26,2005.
Method of Data Analysis: As histopathological find-ings, poorly differentiated atypical cells were noted, andEMA was positive on immunostaining. Based on thesefindings, undifferentiated cancer was diagnosed. No re-gional lymph node and systemic metastasis was noted onCT, MRI, Ga and bone scintigraphy.
Results: As preoperative treatment, radiotherapy at 40Gy and chemotherapy with one course of TS-1 100mg/body x 14 days � CDDP 10 mg/body x 5 days andone course of TS-1 100 mg/body x 14 days were per-formed. Surgery was planned, but not performed be-cause of the strong request of the patient. Additionalirradiation at 30 Gy and chemotherapy with one courseof TS-1 100 mg/body x 14 days � CDDP 10 mg/body x5 days markedly reduced the tumor, and the patient wasdischarged. The patient received 5 courses of chemo-therapy with TS-1 100 mg/body x 14 days at the outpa-tient clinic as of December 2006, and no recurrence wasnoted. The patient is under course observation, and wewill closely follow him.
Conclusion: Radiotherapy and chemotherapy weremarkedly effective for undifferentiated cancer.
Scientific Poster Session
43.e56 AAOMS • 2007
