ANDRES DAVID ARANZAZU CEBALLOSANDRES DAVID ARANZAZU CEBALLOS

3rd SEMESTER3rd SEMESTERFACULTAD DE MEDICINAFACULTAD DE MEDICINA

U.P.BU.P.B

JANUARY 28/2013JANUARY 28/2013

Oxidative stress is Oxidative stress is mainly caused by mainly caused by oxygen radicals (ROS) oxygen radicals (ROS) and is closely related and is closely related to diseases such as to diseases such as diabetes mellitus and diabetes mellitus and cancer.cancer.

Red blood cells which are Red blood cells which are essentially susceptible to essentially susceptible to attack by ROS. attack by ROS.

The antioxidant capacity of The antioxidant capacity of the cells in our bodies have a the cells in our bodies have a key role in the defense and key role in the defense and maintenance of cellular maintenance of cellular homeostasis.homeostasis.

 

Through methods such Through methods such as immunofluorescence as immunofluorescence and exposure to different and exposure to different peroxides, will determine peroxides, will determine whether the cells that are whether the cells that are attacked by these attacked by these radicals, have variation in radicals, have variation in electrical potential.electrical potential.  

I think the study is a breakthrough I think the study is a breakthrough for knowledge about this issue for knowledge about this issue that increasingly is more relatedthat increasingly is more relatedto associated diseases, but hopefullyto associated diseases, but hopefully in the future different cells in the future different cells can be analyzed with this method for can be analyzed with this method for measuring peroxidation and its measuring peroxidation and its influenceinfluence on the membrane potential. on the membrane potential.

Lipids are the main Lipids are the main component of the cell component of the cell membrane, the lipid bilayer membrane, the lipid bilayer is basically composed of is basically composed of lipids, but other lipids, but other macromolecules are also macromolecules are also part of this physical barrier part of this physical barrier and this are the proteins.and this are the proteins.

The major lipid bilayer The major lipid bilayer comprising mainly are: comprising mainly are: phosphatidylcholine phosphatidylcholine phosphatidylethanolamiphosphatidylethanolamine, phospholipids and ne, phospholipids and cholesterol.cholesterol.

Interactions between lipids and proteins leading to different functions as solute transport and solvent particles receptors, intercellular interaction, central to the survival of the cell in their midst.

Using methods such as x-ray fluorescence is Using methods such as x-ray fluorescence is determined the structures of membrane determined the structures of membrane proteins and lipids and their interactions with proteins and lipids and their interactions with the environment either intracellular or the environment either intracellular or extracellularextracellular.  

This study is very important because the structure of de cells and the interaction of yours components is essential for the healthy development of an organism . 

Know by methods inmunofluorenscencia if membrane peroxidation causes changes in membrane potential is useful to find other diseases as diabetes mellitus and cancer and others disorders of cell lysis.

Always intoaccount structures Always intoaccount structures of lipids and proteins and of lipids and proteins and their interaction using their interaction using methods such as x-rays methods such as x-rays cristalometria, has a crucial cristalometria, has a crucial improtancia disaggregate the improtancia disaggregate the functions of lipids and functions of lipids and proteins, their interaction with proteins, their interaction with the cell in addition to the the cell in addition to the body.body.

 Jewell SA, Petrov, PG, Winlove CP. The effect of oxidative stress on the membrane dipole potential of human red blood cells. Biochimica et Biophysica Acta. 2013 Jan 1; 10(12): 10-3p.

Martinez Sanchez, Maria Lina. Molecular Biology. 2. ed. Medellín: UPB. Faculty of Medicine, 2012. 300 p.

Barrera NP, Zhou M, Robinson CV. The role of lipids in defining membrane protein interactions: insights from mass spectrometry. Department of Physiology: Pontificia Universidad Catolica of Chile, 2012. 1-8p.