Platelet Function Testing Kit for each patient The packet contains everything needed We will now...
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Transcript of Platelet Function Testing Kit for each patient The packet contains everything needed We will now...
Platelet Function Testing
Kit for each patient
The packet contains everything needed
We will now demonstrate how to perform the test
insulated pouchheating padconnecting 2way adaptorsfixing solution
aspirin testclopidogrel testbaseline test
Instruction leafletCitrate blood collection tube
Three platelet stimulant tubes
Activate the heating pad by clicking on the circular valve inside
Warming the tubes
When activated the gel turns white and warms up immediately
Warming the tubes
Place the heated pad inside one pocket of the insulation pouch
Warming the tubes
Place the three stimulant tubes in the other pocket of the insulation pouch
Warming the tubes
The stimulant tubes are now ready to use
Warming the tubes
Venepuncture
Select the green topped citrate monovette
Blood should preferably be collected using one of the following venepuncture procedures
ButterflyBD VacutainerSarstedt Multifly set
Butterfly you will also need the Sarstedt multi-adaptor valve
Venepuncture
BD Vacutainer Safety-Lok you also need the Sarstedt multi-adaptor valve
Venepuncture
the Sarstedt multi-adaptor valve
Venepuncture
Sarstedt MultifySet already supplied with the adaptor for the monovette tube
Venepuncture
Filled citrate anticoagulant tube
Fill to the 10 ml line by pulling gently on the plunger (do not lock off the tube)Invert 3 times to mix the blood
Pull the plunger pulled back (do not lock off the tube)Add the blood from the syringe up to the 10 ml markScrew on the green capInvert 3 times to mix the blood
If you have to use a needle and syringe
If using a needle and syringe the tube will need to be opened by holding the monovette upright and unscrewing the green end taking care not to spill the anticoagulant inside.
10 ml mark
Venepuncture
Filled citrate anticoagulant tube
Place the blood in the insulation pouch with the stimulant tubesuntil ready to perform the test
Mix the blood and attach one of the 2way adaptors
Platelet stimulation
Attach each stimulant tube and add blood in turn
Fill three stimulant tubes
Platelet stimulation
To fill, pull back plunger until top of the plunger aligns with bottom arrow
Blood should flow up to4.5 ml mark
Platelet stimulation
Place the stimulant tubes back in the pouch to keep warm
Check the time and wait 5 minutes!
Platelet stimulation
While waiting for the 5 minutes
Discard the used citrate tube and adaptor valve
While waiting for 5 minutes
Take the second 2way adaptor and attach to the Fixing Solution Tube
When 5 minutes is passed
Attach each stimulant tube in turn to the 2way adaptor Pull back plunger all the way and fill the tubes with the fixing solution (liquid aligns with the 4.5 ml mark)
Completed stimulant tubes
Invert to mix
Snap off the plungers on the stimulant tubes
Write patient details on the labels on each of the three stimulant tubes
Place the completed stimulant tubes in the padding for transportation
Place tubes inside the packageand transfer pack to Nottingham
Platelet functionwill be measured asplatelet P-selectin expression